Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusion

scientific article published on December 2004

Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusion is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1110/PS.04931604
P932PMC publication ID2287301
P698PubMed publication ID15557268
P5875ResearchGate publication ID8170588

P50authorBumjoon KimQ65589292
P2093author name stringLi Liu
Ashutosh Chilkoti
Kimberly Trabbic-Carlson
P2860cites workSingle-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferaseQ27860571
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Photoresponsive polymer-enzyme switchesQ34429158
Engineering proteins for purificationQ35285338
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Entropic elastic processes in protein mechanisms. I. Elastic structure due to an inverse temperature transition and elasticity due to internal chain dynamicsQ36429801
Gene fusions for purpose of expression: an introductionQ37945531
Affinity fusion strategies for detection, purification, and immobilization of recombinant proteinsQ41608082
Isolation, purification and cell-free synthesis of calmodulin from the pig anterior pituitary glandQ41971143
Protein purification by fusion with an environmentally responsive elastin-like polypeptide: effect of polypeptide length on the purification of thioredoxin.Q43695737
Characterization of a genetically engineered elastin-like polypeptide for cartilaginous tissue repairQ44128798
The molecular basis of the temperature- and pH-induced conformational transitions in elastin-based peptidesQ44310450
Swelling and Mechanical Behaviors of Chemically Cross-Linked Hydrogels of Elastin-like PolypeptidesQ44437353
Temperature-induced switching of enzyme activity with smart polymer-enzyme conjugatesQ44447813
The molecular basis for the inverse temperature transition of elastinQ47619678
Effect of protein fusion on the transition temperature of an environmentally responsive elastin-like polypeptide: a role for surface hydrophobicity?Q48033543
A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm.Q54663005
An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding proteinQ70261275
Purification of recombinant proteins by fusion with thermally-responsive polypeptidesQ73143445
Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instabilityQ74386329
Phase-structure transitions of the elastin polypentapeptide-water system within the framework of composition-temperature studiesQ93682482
P433issue12
P921main subjectEscherichia coliQ25419
P304page(s)3274-3284
P577publication date2004-12-01
P1433published inProtein ScienceQ7251445
P1476titleExpression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusion
P478volume13

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cites work (P2860)
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