An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases.

scientific article published on 27 April 2011

An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases. is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.1016/J.AB.2011.04.034
P698PubMed publication ID21600185
P5875ResearchGate publication ID51153858

P2093author name stringLixin Ma
Anlong Xu
Shangwu Chen
Hongwei Shao
Lichun Zhang
Dongming Lan
Guangrui Huang
P2860cites workUlp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeastQ27625224
SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins.Q30342324
Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysisQ34324172
Purification of recombinant proteins from Escherichia coli at low expression levels by inverse transition cyclingQ35697361
Free energy transduction in polypeptides and proteins based on inverse temperature transitionsQ35924393
Ultra-high expression of a thermally responsive recombinant fusion protein in E. coliQ36925255
Functional expression of a biologically active fragment of soluble gp130 as an ELP-fusion protein in transgenic plants: purification via inverse transition cyclingQ40276991
Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusionQ41832411
Protein purification by fusion with an environmentally responsive elastin-like polypeptide: effect of polypeptide length on the purification of thioredoxin.Q43695737
Thermally triggered purification and immobilization of elastin-OPH fusionsQ44217009
Self-cleavable stimulus responsive tags for protein purification without chromatographyQ46642250
Heterologous expression and optimized one-step separation of levansucrase via elastin-like polypeptides tagging system.Q48017706
Effect of protein fusion on the transition temperature of an environmentally responsive elastin-like polypeptide: a role for surface hydrophobicity?Q48033543
Simultaneous phase transition of ELP tagged molecules and free ELP: an efficient and reversible capture system.Q51135486
Simple bioseparations using self-cleaving elastin-like polypeptide tags.Q51381897
Substrate requirements of human rhinovirus 3C protease for peptide cleavage in vitroQ68461470
Purification of recombinant proteins by fusion with thermally-responsive polypeptidesQ73143445
P433issue2
P407language of work or nameEnglishQ1860
P304page(s)200-202
P577publication date2011-04-27
P1433published inAnalytical BiochemistryQ485215
P1476titleAn improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases.
P478volume415

Reverse relations

cites work (P2860)
Q90382859Application of short hydrophobic elastin-like polypeptides for expression and purification of active proteins
Q38224685Applications of elastin-like polypeptides in drug delivery.
Q42933145Non-chromatographic purification of recombinant elastin-like polypeptides and their fusions with peptides and proteins from Escherichia coli
Q36327788Stable plastid transformation for high-level recombinant protein expression: promises and challenges.
Q38145658Stimuli responsive elastin-like polypeptides and applications in medicine and biotechnology
Q40229359Tobacco etch virus protease mediating cleavage of the cellulose-binding module tagged colored proteins immobilized on the regenerated amorphous cellulose

Search more.