Mass spectrometry-based relative quantification of proteins in precatalytic and catalytically active spliceosomes by metabolic labeling (SILAC), chemical labeling (iTRAQ), and label-free spectral count

scientific article published on 21 January 2014

Mass spectrometry-based relative quantification of proteins in precatalytic and catalytically active spliceosomes by metabolic labeling (SILAC), chemical labeling (iTRAQ), and label-free spectral count is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.1261/RNA.041244.113
P932PMC publication ID3923134
P698PubMed publication ID24448447
P5875ResearchGate publication ID259846658

P50authorReinhard Georg LührmannQ1317944
Henning UrlaubQ28037184
Carla SchmidtQ43095063
P2093author name stringMads Grønborg
Jochen Deckert
Sergey Bessonov
Thomas Conrad
P2860cites workA subset of human 35S U5 proteins, including Prp19, function prior to catalytic step 1 of splicingQ24294927
Purification and characterization of native spliceosomes suitable for three-dimensional structural analysisQ24296676
Composition and three-dimensional EM structure of double affinity-purified, human prespliceosomal A complexesQ24298731
The cellular EJC interactome reveals higher-order mRNP structure and an EJC-SR protein nexusQ24301338
Structure of the Y14-Magoh core of the exon junction complexQ24303672
Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicingQ24317157
The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctionsQ24595096
Functional analysis of the human CDC5L complex and identification of its components by mass spectrometryQ24596551
Large-scale proteomic analysis of the human spliceosomeQ24671785
Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide GelsQ27860531
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nucleiQ27860728
The Prp19p-associated complex in spliceosome activationQ27938109
Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomicsQ28131742
The spliceosome: design principles of a dynamic RNP machineQ28131809
Mass spectrometry-based proteomicsQ28182890
Comprehensive proteomic analysis of the human spliceosomeQ28201545
Quantitative mass spectrometry in proteomics: a critical reviewQ28238800
Proteomics by mass spectrometry: approaches, advances, and applicationsQ28243072
Quantitative mass spectrometry-based proteomics: an overviewQ28268087
Mass spectrometry-based proteomics turns quantitativeQ28291379
Pre-mRNA splicing: awash in a sea of proteinsQ29547272
A model for random sampling and estimation of relative protein abundance in shotgun proteomicsQ29547316
Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagentsQ29547450
Parts per million mass accuracy on an Orbitrap mass spectrometer via lock mass injection into a C-trapQ29615742
Mass spectrometry and protein analysisQ33239890
Conservation of the protein composition and electron microscopy structure of Drosophila melanogaster and human spliceosomal complexesQ33382329
Characterization of purified human Bact spliceosomal complexes reveals compositional and morphological changes during spliceosome activation and first step catalysisQ34146146
Semiquantitative proteomic analysis of the human spliceosome via a novel two-dimensional gel electrophoresis methodQ34181884
The ABC's (and XYZ's) of peptide sequencingQ34344848
The evolutionarily conserved core design of the catalytic activation step of the yeast spliceosomeQ35014709
"Nought may endure but mutability": spliceosome dynamics and the regulation of splicingQ37197189
Quantitative mass spectrometry in proteomics: critical review update from 2007 to the presentQ38024637
Protein composition and electron microscopy structure of affinity-purified human spliceosomal B complexes isolated under physiological conditionsQ38406412
Chromatin affinity purification and quantitative mass spectrometry defining the interactome of histone modification patterns.Q39451519
Direct comparison of stable isotope labeling by amino acids in cell culture and spectral counting for quantitative proteomics.Q39655280
The second catalytic step of pre-mRNA splicingQ40939314
Protein composition of human mRNPs spliced in vitro and differential requirements for mRNP protein recruitmentQ41500093
Dissection of the factor requirements for spliceosome disassembly and the elucidation of its dissociation products using a purified splicing systemQ41553559
Comparison of spectral counting and metabolic stable isotope labeling for use with quantitative microbial proteomicsQ42043971
Small nuclear ribonucleoprotein remodeling during catalytic activation of the spliceosome.Q50336089
Isolation of an active step I spliceosome and composition of its RNP core.Q50336090
Systematic comparison of label-free, metabolic labeling, and isobaric chemical labeling for quantitative proteomics on LTQ Orbitrap Velos.Q51467338
An automated in-gel digestion/iTRAQ-labeling workflow for robust quantification of gel-separated proteinsQ57590475
iTRAQ-Labeling of In-Gel Digested Proteins for Relative QuantificationQ57590482
P433issue3
P407language of work or nameEnglishQ1860
P921main subjectspliceosomeQ915868
P304page(s)406-420
P577publication date2014-01-21
P1433published inRNAQ7277164
P1476titleMass spectrometry-based relative quantification of proteins in precatalytic and catalytically active spliceosomes by metabolic labeling (SILAC), chemical labeling (iTRAQ), and label-free spectral count
P478volume20

Reverse relations

cites work (P2860)
Q41510811A new role for FBP21 as regulator of Brr2 helicase activity
Q35961918CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex
Q36514597Differential dynamics of the mammalian mRNA and protein expression response to misfolding stress.
Q47161845Dynamic changes of urine proteome in a Walker 256 tumor-bearing rat model
Q90262135Early urine proteome changes in the Walker-256 tail-vein injection rat model
Q33727141Effects of arginine vasopressin on the urine proteome in rats
Q52668696Identifying RNA splicing factors using IFT genes in Chlamydomonas reinhardtii.
Q37401085Identifying proteins that bind to specific RNAs - focus on simple repeat expansion diseases
Q97681153Mutations in FAM50A suggest that Armfield XLID syndrome is a spliceosomopathy
Q91929009Overview of Extracellular Vesicles, Their Origin, Composition, Purpose, and Methods for Exosome Isolation and Analysis
Q38955489Proteomic Analysis of Postsynaptic Protein Complexes Underlying Neuronal Plasticity.
Q37642133Proteomics progresses in microbial physiology and clinical antimicrobial therapy
Q40525343Quantitative Proteomics Reveals the Defense Response of Wheat against Puccinia striiformis f. sp. tritici
Q93355206RNA Splicing and Disease: Animal Models to Therapies
Q34072259Supraspliceosomes at defined functional states portray the pre-assembled nature of the pre-mRNA processing machine in the cell nucleus
Q52865368The GYF domain protein CD2BP2 is critical for embryogenesis and podocyte function.
Q89882194The complex genetic landscape of familial MDS and AML reveals pathogenic germline variants
Q58608310The neurodegenerative diseases ALS and SMA are linked at the molecular level via the ASC-1 complex
Q26741296The nuts and bolts of the endogenous spliceosome
Q42929753Uncovering the stoichiometry of Pyrococcus furiosus RNase P, a multi-subunit catalytic ribonucleoprotein complex, by surface-induced dissociation and ion mobility mass spectrometry
Q99406301Urinary biomarker discovery in gliomas using mass spectrometry-based clinical proteomics
Q35197926Urinary candidate biomarker discovery in a rat unilateral ureteral obstruction model.
Q90428158Urine proteome changes in rats subcutaneously inoculated with approximately ten tumor cells

Search more.