Use of a dominant rpsL allele conferring streptomycin dependence for positive and negative selection in Thermus thermophilus

scientific article published on 29 June 2007

Use of a dominant rpsL allele conferring streptomycin dependence for positive and negative selection in Thermus thermophilus is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1128/AEM.00751-07
P932PMC publication ID1950985
P698PubMed publication ID17601820

P50authorJesús MendietaQ42238581
P2093author name stringFelipe Cava
José Berenguer
Eduardo López-Viñas
Emilio Blas-Galindo
P2860cites workThe genome sequence of the extreme thermophile Thermus thermophilusQ22122105
SWISS-MODEL: An automated protein homology-modeling serverQ24672647
Structure of the 30S ribosomal subunitQ27627261
Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibioticsQ27627265
Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 A resolutionQ27638951
Selection of tRNA by the ribosome requires a transition from an open to a closed formQ27640089
Studies on transformation of Escherichia coli with plasmidsQ27860598
Structure of the 70S ribosome complexed with mRNA and tRNAQ27860602
SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modelingQ27860614
WHAT IF: a molecular modeling and drug design programQ27860734
Errors in protein structuresQ27860776
Hyperthermophilic enzymes: sources, uses, and molecular mechanisms for thermostabilityQ28203641
ProMod and Swiss-Model: Internet-based tools for automated comparative protein modellingQ29617090
A high-transformation-efficiency cloning vector for Thermus thermophilusQ30805031
Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8.Q33789177
Molecular analyses of the natural transformation machinery and identification of pilus structures in the extremely thermophilic bacterium Thermus thermophilus strain HB27.Q34100377
Genetic transformation of the extreme thermophile Thermus thermophilus and of other Thermus spp.Q34196779
pH-dependent conformational switch activates the inhibitor of transcription elongation.Q34619759
Development of plasmid cloning vectors for Thermus thermophilus HB8: expression of a heterologous, plasmid-borne kanamycin nucleotidyltransferase geneQ35950568
Anaerobic growth, a property horizontally transferred by an Hfr-like mechanism among extreme thermophiles.Q39569283
Development of Thermus-Escherichia shuttle vectors and their use for expression of the Clostridium thermocellum celA gene in Thermus thermophilusQ39940421
Cloning of alpha- and beta-galactosidase genes from an extreme thermophile, Thermus strain T2, and their expression in Thermus thermophilus HB27.Q42030940
In silico activation of Src tyrosine kinase reveals the molecular basis for intramolecular autophosphorylationQ42635710
A thermophilic nitrate reductase is responsible for the strain specific anaerobic growth of Thermus thermophilus HB8.Q42675632
Membrane-associated maturation of the heterotetrameric nitrate reductase of Thermus thermophilusQ42863217
Insertional mutagenesis in the extreme thermophilic eubacteria Thermus thermophilus HB8.Q43016848
Biochemical and regulatory properties of a respiratory island encoded by a conjugative plasmid in the extreme thermophile Thermus thermophilusQ43019350
Streptomycin-resistant and streptomycin-dependent mutants of the extreme thermophile Thermus thermophilusQ43028784
The periplasmic space in Thermus thermophilus: evidence from a regulation-defective S-layer mutant overexpressing an alkaline phosphataseQ43030667
Development of a gene expression vector for Thermus thermophilus based on the promoter of the respiratory nitrate reductaseQ43032463
Identifying protein construct variants with increased crystallization propensity--a case studyQ43043526
A cytochrome c encoded by the nar operon is required for the synthesis of active respiratory nitrate reductase in Thermus thermophilusQ44068367
Binding to pyruvylated compounds as an ancestral mechanism to anchor the outer envelope in primitive bacteriaQ44854356
Characterization of a plasmid replicative origin from an extreme thermophileQ48029788
Double, independent mutational events in the rpsL gene of Escherichia coli: an example of hypermutability?Q54654746
An efficient gene replacement and deletion system for an extreme thermophile, Thermus thermophilusQ77396895
P433issue16
P407language of work or nameEnglishQ1860
P921main subjectThermus thermophilusQ139470
streptomycinQ192717
P304page(s)5138-5145
P577publication date2007-06-29
P1433published inApplied and Environmental MicrobiologyQ4781593
P1476titleUse of a dominant rpsL allele conferring streptomycin dependence for positive and negative selection in Thermus thermophilus
P478volume73

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cites work (P2860)
Q48177430Cre/lox-based multiple markerless gene disruption in the genome of the extreme thermophile Thermus thermophilus.
Q38680095Curing the Megaplasmid pTT27 from Thermus thermophilus HB27 and Maintaining Exogenous Plasmids in the Plasmid-Free Strain
Q92632543Development of a markerless gene deletion strategy using rpsL as a counterselectable marker and characterization of the function of RA0C_1534 in Riemerella anatipestifer ATCC11845 using this strategy
Q35107273Engineering the genome of Thermus thermophilus using a counterselectable marker
Q43015159Homogeneous incorporation of secondary cell wall polysaccharides to the cell wall of Thermus thermophilus HB27.
Q38387024Markerless Gene Deletion with Cytosine Deaminase in Thermus thermophilus Strain HB27.
Q92833461Selection-free markerless genome manipulations in the polyploid bacterium Thermus thermophilus
Q30374445Thermus thermophilus as biological model.

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