Rapid and direct quantitative detection of viable bifidobacteria in probiotic yogurt by combination of ethidium monoazide and real-time PCR using a molecular beacon approach

scientific article published on September 8, 2010

Rapid and direct quantitative detection of viable bifidobacteria in probiotic yogurt by combination of ethidium monoazide and real-time PCR using a molecular beacon approach is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.1017/S0022029910000658
P953full work available at URLhttps://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0022029910000658
P698PubMed publication ID20822571

P2093author name stringC. Wang
R. Pang
X. C. Meng
L. Q. Wang
P2860cites workDevelopment and application of new nucleic acid-based technologies for microbial community analyses in foodsQ30847078
Use of ethidium monoazide and PCR in combination for quantification of viable and dead cells in complex samplesQ33788896
Ethidium Monoazide for DNA-Based Differentiation of Viable and Dead Bacteria by 5′-Nuclease PCRQ34191545
Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cellsQ34534512
Identification and quantification of Bifidobacterium species isolated from food with genus-specific 16S rRNA-targeted probes by colony hybridization and PCRQ35199207
Quantitative PCR with 16S rRNA-gene-targeted species-specific primers for analysis of human intestinal bifidobacteria.Q35539776
From bacterial genome to functionality; case bifidobacteriaQ36880921
Spectroscopic properties of ethidium monoazide: a fluorescent photoaffinity label for nucleic acidsQ40576840
New real-time quantitative PCR procedure for quantification of bifidobacteria in human fecal samplesQ41031523
Selective removal of DNA from dead cells of mixed bacterial communities by use of ethidium monoazideQ41772275
Molecular-beacon multiplex real-time PCR assay for detection of Vibrio choleraeQ41826511
Enumeration of viable Listeria monocytogenes cells by real-time PCR with propidium monoazide and ethidium monoazide in the presence of dead cellsQ42909274
Quantitative detection of probiotic Bifidobacterium strains in bacterial mixtures by using real-time PCR.Q44515740
A PCR method for detection of bifidobacteria in raw milk and raw milk cheese: comparison with culture-based methodsQ45241807
Detection of viable and dead Listeria monocytogenes on gouda-like cheeses by real-time PCR.Q45308011
Detection of viable Escherichia coli O157:H7 by ethidium monoazide real-time PCR.Q46003893
Selective PCR detection of viable Enterobacter sakazakii cells utilizing propidium monoazide or ethidium bromide monoazideQ46485629
Insufficient differentiation of live and dead Campylobacter jejuni and Listeria monocytogenes cells by ethidium monoazide (EMA) compromises EMA/real-time PCR.Q46593336
Evaluation of real-time PCR targeting the 16S rRNA and recA genes for the enumeration of bifidobacteria in probiotic productsQ49069963
A PCR-based method for identification of bifidobacteria from the human alimentary tract at the species level.Q54765024
Quantitative assessment of faecal bifidobacterial populations by real-time PCR using lanthanide probesQ57717172
Bacterial composition of commercial probiotic products as evaluated by PCR-DGGE analysisQ59418454
A selective enumeration medium for bifidobacteria in fermented dairy productsQ70993887
An enzymatic-colorimetric assay for the quantification of BifidobacteriumQ73547264
Note: evaluation of selective media for the enumeration of Bifidobacterium sp. in milkQ74577809
P433issue4
P407language of work or nameEnglishQ1860
P921main subjectyogurtQ13317
probioticsQ1816730
P304page(s)498-504
P577publication date2010-09-08
P1433published inJournal of Dairy ResearchQ2049609
P1476titleRapid and direct quantitative detection of viable bifidobacteria in probiotic yogurt by combination of ethidium monoazide and real-time PCR using a molecular beacon approach
P478volume77

Reverse relations

cites work (P2860)
Q35840710Contemporary nucleic acid-based molecular techniques for detection, identification, and characterization of Bifidobacterium
Q37564221Evolution of microbiological analytical methods for dairy industry needs
Q39764883Quantitative detection of viable Bifidobacterium bifidum BF-1 cells in human feces by using propidium monoazide and strain-specific primers
Q38151969Recent developments in the use of viability dyes and quantitative PCR in the food microbiology field

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