Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs

scientific article

Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1016/0092-8674(86)90066-8
P698PubMed publication ID2944598
P5875ResearchGate publication ID20210857

P2093author name stringSharp PA
Konarska MM
P433issue6
P407language of work or nameEnglishQ1860
P1104number of pages11
P304page(s)845-855
P577publication date1986-09-01
P1433published inCellQ655814
P1476titleElectrophoretic separation of complexes involved in the splicing of precursors to mRNAs
P478volume46

Reverse relations

cites work (P2860)
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Q40919235Control of transferrin receptor expression via nitric oxide-mediated modulation of iron-regulatory protein 2.
Q37492260Cytoplasmic protein binding to highly conserved sequences in the 3' untranslated region of mouse protamine 2 mRNA, a translationally regulated transcript of male germ cells
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Q35076313Deletion of MUD2, the yeast homolog of U2AF65, can bypass the requirement for sub2, an essential spliceosomal ATPase
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Q40918044Development of novel aroylhydrazone ligands for iron chelation therapy: 2-pyridylcarboxaldehyde isonicotinoyl hydrazone analogs
Q35930823Differential ASF/SF2 activity in extracts from normal WI38 and transformed WI38VA13 cells
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Q36958383Differential block of U small nuclear ribonucleoprotein particle interactions during in vitro splicing of adenovirus E1A transcripts containing abnormally short introns
Q36841865Discontinuous native protein gel electrophoresis: pros and cons
Q41201011Discrete domains of human U6 snRNA required for the assembly of U4/U6 snRNP and splicing complexes
Q36835030Distinct effects on splicing of two monoclonal antibodies directed against the amino-terminal domain of galectin-3.
Q40535866Domains required for nucleic acid binding activities in chloroplast ribonucleoproteins
Q73485917Dynamics of the U1 Small Nuclear Ribonucleoprotein during Yeast Spliceosome Assembly
Q24627964Early commitment of yeast pre-mRNA to the spliceosome pathway
Q40647569Effect of 5' splice site mutations on splicing of the preceding intron
Q35996295Electron microscopic identification of the yeast spliceosome
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Q41941626Engagement of tumor necrosis factor mRNA by an endotoxin-inducible cytoplasmic protein.
Q37316124Evidence for a base-pairing interaction between U6 small nuclear RNA and 5' splice site during the splicing reaction in yeast
Q41974221Evidence for an RNA chaperone function of polypyrimidine tract-binding protein in picornavirus translation
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Q40420630Evolutionary conserved multiprotein complexes interact with the 3' untranslated region of histone transcripts
Q36713104Exon definition may facilitate splice site selection in RNAs with multiple exons
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Q24528154Extracellular RNA is a natural cofactor for the (auto-)activation of Factor VII-activating protease (FSAP)
Q36777141Factor interactions with the simian virus 40 early pre-mRNA influence branch site selection and alternative splicing
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Q35228128Functional analysis of point mutations in the AAUAAA motif of the SV40 late polyadenylation signal
Q24596551Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry
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Q22254390Functional association of U2 snRNP with the ATP-independent spliceosomal complex E
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Q39108397Genetics and biochemistry remain essential in the structural era of the spliceosome
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Q41094215Heat shock but not other stress inducers leads to the disruption of a sub-set of snRNPs and inhibition of in vitro splicing in HeLa cells.
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Q34311067Hydroxyl radical "footprinting" of RNA: application to pre-mRNA splicing complexes
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Q33651382Identification of a functional mammalian spliceosome containing unspliced pre-mRNA
Q34723405Identification of a novel sequence that governs both polyadenylation and alternative splicing in region E3 of adenovirus
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Q36962386Identification of cis-acting intron and exon regions in influenza virus NS1 mRNA that inhibit splicing and cause the formation of aberrantly sedimenting presplicing complexes
Q37701736Identification of five putative yeast RNA helicase genes
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Q34626768Identification of two proteins that bind to a pyrimidine-rich sequence in the 3'-untranslated region of GAP-43 mRNA
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Q35851124In vitro capping in Trypanosoma cruzi identifies and shows specificity for the spliced leader RNA and U-RNAs
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Q45857699Interactions between viral coat protein and a specific binding region on turnip crinkle virus RNA
Q27938316Interactions of PRP2 protein with pre-mRNA splicing complexes in Saccharomyces cerevisiae
Q35827075Interplay between U2 snRNP and 3' splice factor(s) for branch point selection on human beta-globin pre-mRNA
Q40645992Introduction of functional artificial introns into the naturally intronless ura4 gene of Schizosaccharomyces pombe
Q41469835Intron definition in splicing of small Drosophila introns
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Q38342736Mapping U2 snRNP--pre-mRNA interactions using biotinylated oligonucleotides made of 2'-OMe RNA.
Q33746053Mechanism for cryptic splice site activation during pre-mRNA splicing
Q34963359Mechanistic control of carcinoembryonic antigen-related cell adhesion molecule-1 (CEACAM1) splice isoforms by the heterogeneous nuclear ribonuclear proteins hnRNP L, hnRNP A1, and hnRNP M.
Q47738832Mechanistic insights into precursor messenger RNA splicing by the spliceosome
Q58324056Metal ion catalysis during splicing of premessenger RNA
Q26738664Modulating splicing with small molecular inhibitors of the spliceosome
Q34473874Modulation of 5' splice site choice in pre-messenger RNA by two distinct steps
Q33919386Multiple domains of U1 snRNA, including U1 specific protein binding sites, are required for splicing
Q36833512Multiple interactions between the splicing substrate and small nuclear ribonucleoproteins in spliceosomes
Q22254669Multisite RNA binding and release of polypyrimidine tract binding protein during the regulation of c-src neural-specific splicing
Q27486572Mutagenesis of the 3' nontranslated region of Sindbis virus RNA
Q97681153Mutations in FAM50A suggest that Armfield XLID syndrome is a spliceosomopathy
Q40504202Nucleic acid-binding specificities of tobacco chloroplast ribonucleoproteins
Q38333751Nuleclear extracts of Crithidia fasciculata contain a factor(s) that binds to the 5'-untranslated regions of TOP2 and RPA1 mRNAs containing sequences required for their cell cycle regulation
Q33721453Oxidation-reduction sensitive interaction of a cellular 50-kDa protein with an RNA hairpin in the 5' noncoding region of the poliovirus genome
Q67749570Oxidation-reduction-sensitive binding of lung protein to rat catalase mRNA
Q36714216PRP18, a protein required for the second reaction in pre-mRNA splicing
Q36759976PRP4: a protein of the yeast U4/U6 small nuclear ribonucleoprotein particle
Q24539523PUF60: a novel U2AF65-related splicing activity
Q43594149Parameters that affect in vitro splicing of bovine papillomavirus type 1 late pre-mRNAs
Q38833488Pathogenic variants that alter protein code often disrupt splicing.
Q24528188Peptides from the calcitonin genes: molecular genetics, structure and function
Q40421957Phosphorothioate substitution identifies phosphate groups important for pre-mRNA splicing
Q38076663Pick one, but be quick: 5' splice sites and the problems of too many choices
Q54981229Polyadenylation precedes splicing in vitro
Q36275061Polypyrimidine track-binding protein binding downstream of caspase-2 alternative exon 9 represses its inclusion
Q36481243Position-dependent splicing activation and repression by SR and hnRNP proteins rely on common mechanisms.
Q35071246Post-transcriptional regulation of transferrin receptor mRNA by IFNγ
Q40530952Potential role of poly(A) polymerase in the assembly of polyadenylation-specific RNP complexes
Q40817226Pre-mRNA splicing mutants of Schizosaccharomyces pombe
Q33933536Prespliceosomal assembly on microinjected precursor mRNA takes place in nuclear speckles
Q38290577Probing interactions between the U2 small nuclear ribonucleoprotein and the DEAD-box protein, Prp5.
Q33847099Protein composition of mammalian spliceosomes assembled in vitro
Q28251112Protein-protein interactions and 5'-splice-site recognition in mammalian mRNA precursors
Q40506244Proteolysis of splicing factors during rat and monkey cell fractionation
Q35891571Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors
Q26865050Pseudouridines in spliceosomal snRNAs
Q24296676Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis
Q43415010Purification and visualization of native spliceosomes
Q46194447Purification of the spliceosome A-complex and its visualization by electron microscopy
Q35234756Purified U5 small nuclear ribonucleoprotein can relieve the inhibition of spliceosome assembly and splicing by snRNP-free nuclear proteins
Q37996957RNA biology in a test tube--an overview of in vitro systems/assays
Q43182216RNA-protein interactions in the nuclei of Xenopus oocytes: complex formation and processing activity on the regulatory intron of ribosomal protein gene L1.
Q36790107RNA11 protein is associated with the yeast spliceosome and is localized in the periphery of the cell nucleus
Q50911751Radical probing of spliceosome assembly.
Q34362294Reassembly and protection of small nuclear ribonucleoprotein particles by heat shock proteins in yeast cells
Q40678411Reconstitution of functional mammalian U4 small nuclear ribonucleoprotein: Sm protein binding is not essential for splicing in vitro
Q42146150Reduced fidelity of branch point recognition and alternative splicing induced by the anti-tumor drug spliceostatin A
Q26827257Regulation of brain iron and copper homeostasis by brain barrier systems: implication in neurodegenerative diseases
Q41480583Regulation of transferrin receptor mRNA expression. Distinct regulatory features in erythroid cells
Q41904897Repression of prespliceosome complex formation at two distinct steps by Fox-1/Fox-2 proteins
Q40509548Requirement of protein factors and ATP for the disassembly of the splicesome after mRNA splicing reaction
Q35925510Requirements for U2 snRNP addition to yeast pre-mRNA.
Q34362249Resolution of the mammalian E complex and the ATP-dependent spliceosomal complexes on native agarose mini-gels
Q39698187Reversible inhibition of the second step of splicing suggests a possible role of zinc in the second step of splicing
Q36218578Ribonucleoprotein particles in cellular processes
Q37143270Role of adenine functional groups in the recognition of the 3'-splice-site AG during the second step of pre-mRNA splicing
Q36763488Role of poly(A) polymerase in the cleavage and polyadenylation of mRNA precursor
Q35864651Role of the branch site/3'-splice site region in adenovirus-2 E1A pre-mRNA alternative splicing: evidence for 5'- and 3'-splice site co-operation
Q41533943Roles of PRP8 protein in the assembly of splicing complexes
Q41514780Roles of U4 and U6 snRNAs in the assembly of splicing complexes.
Q35902840SC35-mediated reconstitution of splicing in U2AF-depleted nuclear extract
Q24298242SNEV is an evolutionarily conserved splicing factor whose oligomerization is necessary for spliceosome assembly
Q33944960SPF30 is an essential human splicing factor required for assembly of the U4/U5/U6 tri-small nuclear ribonucleoprotein into the spliceosome
Q36780246Sedimentation analysis of polyadenylation-specific complexes
Q36725629Sequence requirements in different steps of the pre-mRNA splicing reaction: analysis by the RNA modification-exclusion technique
Q38678938Sequences upstream of the branch site are required to form helix II between U2 and U6 snRNA in a trans-splicing reaction
Q34693458Sequential recognition of the pre-mRNA branch point by U2AF65 and a novel spliceosome-associated 28-kDa protein
Q37042668Single molecule analysis reveals reversible and irreversible steps during spliceosome activation
Q24324201Sip1, a novel RS domain-containing protein essential for pre-mRNA splicing
Q41357772Specific accessory sequences in Saccharomyces cerevisiae introns control assembly of pre-mRNAs into spliceosomes
Q28345098Specific cleavage of hyper-edited dsRNAs
Q28262042Specific commitment of different pre-mRNAs to splicing by single SR proteins
Q41360882Specific pre-cleavage and post-cleavage complexes involved in the formation of SV40 late mRNA 3' termini in vitro
Q36738496Splice site choice in a complex transcription unit containing multiple inefficient polyadenylation signals
Q34132148Spliceosomal snRNA modifications and their function
Q28646807Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein
Q37099916Spliceosome assembly pathways for different types of alternative splicing converge during commitment to splice site pairing in the A complex.
Q24295147Splicing factor hSlu7 contains a unique functional domain required to retain the protein within the nucleus
Q41288678Splicing of precursors to mRNA in higher plants: mechanism, regulation and sub-nuclear organisation of the spliceosomal machinery
Q33598477Splicing reporter mice revealed the evolutionally conserved switching mechanism of tissue-specific alternative exon selection
Q31121644Symmetrical dimethylarginine methylation is required for the localization of SMN in Cajal bodies and pre-mRNA splicing.
Q36923594TFIIIA binds to different domains of 5S RNA and the Xenopus borealis 5S RNA gene
Q39716206The 5' and 3' splice sites come together via a three dimensional diffusion mechanism
Q38336469The 5′ end domain of U2 snRNA is required to establish the interaction of U2 snRNP with U2 auxiliary factor(s) during mammalian spliceosome assembly
Q28359568The ATP requirement for U2 snRNP addition is linked to the pre-mRNA region 5' to the branch site
Q40645782The RNA polymerase C-terminal domain: a new role in spliceosome assembly
Q33887645The RNA splicing factor hSlu7 is required for correct 3' splice-site choice
Q37578111The Rex regulatory protein of human T-cell lymphotropic virus type I binds specifically to its target site within the viral RNA
Q33886376The SR splicing factors ASF/SF2 and SC35 have antagonistic effects on intronic enhancer-dependent splicing of the beta-tropomyosin alternative exon 6A
Q36430501The Saccharomyces cerevisiae PRP21 gene product is an integral component of the prespliceosome
Q36797057The antigen of hepatitis delta virus: examination of in vitro RNA-binding specificity
Q40022468The branchpoint residue is recognized during commitment complex formation before being bulged out of the U2 snRNA-pre-mRNA duplex
Q37563624The calcitonin gene peptides: biology and clinical relevance
Q40442143The carboxyl terminus of vertebrate poly(A) polymerase interacts with U2AF 65 to couple 3'-end processing and splicing
Q39648201The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells
Q38322227The effect of intracellular iron concentration and nitrogen monoxide on Nramp2 expression and non-transferrin-bound iron uptake
Q33778676The exon splicing silencer in human immunodeficiency virus type 1 Tat exon 3 is bipartite and acts early in spliceosome assembly
Q28610566The first pre-rRNA-processing event occurs in a large complex: analysis by gel retardation, sedimentation, and UV cross-linking
Q35073405The formation of mitochondrial ribonucleoprotein complexes involving guide RNA molecules in Trypanosoma brucei
Q39645951The hnRNP A1 protein regulates HIV-1 tat splicing via a novel intron silencer element.
Q36679792The integrity of the stem structure of human immunodeficiency virus type 1 Tat-responsive sequence of RNA is required for interaction with the interferon-induced 68,000-Mr protein kinase
Q24556544The molecular characterization of PRP6 and PRP9 yeast genes reveals a new cysteine/histidine motif common to several splicing factors
Q36724069The multiple RNA-binding domains of the mRNA poly(A)-binding protein have different RNA-binding activities
Q36573834The newt ribozyme is part of a riboprotein complex
Q39576977The pre-mRNA 5' cap determines whether U6 small nuclear RNA succeeds U1 small nuclear ribonucleoprotein particle at 5' splice sites
Q40693549The protein kinase C beta II exon confers mRNA instability in the presence of high glucose concentrations
Q36798600The ribosome-in-pieces: binding of elongation factor EF-G to oligoribonucleotides that mimic the sarcin/ricin and thiostrepton domains of 23S ribosomal RNA.
Q39481136The role of small nuclear ribonucleoprotein particles in pre-mRNA splicing
Q24601223The spliceosome assembly pathway in mammalian extracts
Q44983925The thermodynamic patterns of eukaryotic genes suggest a mechanism for intron-exon recognition.
Q42264162The transition in spliceosome assembly from complex E to complex A purges surplus U1 snRNPs from alternative splice sites
Q78026434The upstream stem-loop domain of the 3' untranslated region of apolipoprotein II mRNA binds the estrogen-regulated mRNA stabilizing factor
Q36676911The yeast PRP19 protein is not tightly associated with small nuclear RNAs, but appears to associate with the spliceosome after binding of U2 to the pre-mRNA and prior to formation of the functional spliceosome
Q41080746The yeast branchpoint sequence is not required for the formation of a stable U1 snRNA-pre-mRNA complex and is recognized in the absence of U2 snRNA.
Q40048449Three different cellular proteins bind to complementary sites on the 5'-end-positive and 3'-end-negative strands of mouse hepatitis virus RNA.
Q41081059Three protein factors (SF1, SF3 and U2AF) function in pre-splicing complex formation in addition to snRNPs
Q35846398Three recognition events at the branch-site adenine.
Q28584078Thymidylate synthase protein and p53 mRNA form an in vivo ribonucleoprotein complex
Q33993090Transcriptional activation of the chlorocatechol degradative genes of Ralstonia eutropha NH9.
Q27932331Transient interaction of BBP/ScSF1 and Mud2 with the splicing machinery affects the kinetics of spliceosome assembly
Q38310107Translation of 15-lipoxygenase mRNA is inhibited by a protein that binds to a repeated sequence in the 3' untranslated region
Q41552377Translational Regulation of Human Beta Interferon mRNA: Association of the 3' AU-rich Sequence with the Poly(A) Tail Reduces Translation Efficiency in Vitro
Q44333035Triiodothyronine-mediated myosin heavy chain gene transcription in the heart
Q36707298Two cellular proteins bind specifically to a purine-rich sequence necessary for the destabilization function of a c-fos protein-coding region determinant of mRNA instability
Q41342217Two spliceosomes can form simultaneously and independently on synthetic double-intron messenger RNA precursors
Q36818542U-rich sequence-binding proteins (URBPs) interacting with a 20-nucleotide U-rich sequence in the 3' untranslated region of c-fos mRNA may be involved in the first step of c-fos mRNA degradation
Q34761085U1 SnRNP association with HnRNP involves an initial non-specific splice-site independent interaction of U1 SnRNP protein with HnRNA.
Q36919767U1 small nuclear ribonucleoproteins are required early during spliceosome assembly
Q67901761U1 snRNP can influence 3'-splice site selection as well as 5'-splice site selection
Q40395456U1 snRNP-ASF/SF2 interaction and 5' splice site recognition: characterization of required elements.
Q33850531UACUAAC is the preferred branch site for mammalian mRNA splicing
Q36684488Uncoupling Two Functions of the U1 Small Nuclear Ribonucleoprotein Particle During in Vitro Splicing
Q35646639Understanding the biochemical activities of galectin-1 and galectin-3 in the nucleus
Q37693741Upregulation of DMT1 expression in choroidal epithelia of the blood-CSF barrier following manganese exposure in vitro
Q35655392Use of gel retardation to analyze protein-nucleic acid interactions
Q33539202Utilization of enzyme-substrate interactions in analytical chemistry
Q37912004Where splicing joins chromatin.
Q34694763Yeast pre-mRNA splicing: methods, mechanisms, and machinery
Q36878254cis-acting intron mutations that affect the efficiency of avian retroviral RNA splicing: implication for mechanisms of control.
Q36706333cis-acting sequences involved in exon selection in the chicken beta-tropomyosin gene
Q47316387hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B.

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