Single molecule analysis reveals reversible and irreversible steps during spliceosome activation

scientific article published on 31 May 2016

Single molecule analysis reveals reversible and irreversible steps during spliceosome activation is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.7554/ELIFE.14166
P932PMC publication ID4922858
P698PubMed publication ID27244240

P50authorLarry J FriedmanQ42408756
Margaret L RodgersQ55149600
Jeff GellesQ57654942
Melissa J. MooreQ85954688
Aaron A HoskinsQ88825082
P2860cites workCwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centreQ24301865
Early commitment of yeast pre-mRNA to the spliceosome pathwayQ24627964
Visualizing cellular machines with colocalization single molecule microscopyQ26866213
Structure of a yeast spliceosome at 3.6-angstrom resolutionQ27701867
RNA unwinding in U4/U6 snRNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2Q27930438
A spliceosomal recycling factor that reanneals U4 and U6 small nuclear ribonucleoprotein particlesQ27931035
Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domainQ27932833
The DExD/H-box ATPase Prp2p destabilizes and proofreads the catalytic RNA core of the spliceosomeQ27933083
Splicing proofreading at 5' splice sites by ATPase Prp28pQ27936858
Arrested yeast splicing complexes indicate stepwise snRNP recruitment during in vivo spliceosome assemblyQ27937218
Yeast precursor mRNA processing protein PRP19 associates with the spliceosome concomitant with or just after dissociation of U4 small nuclear RNA.Q27937247
The Prp19p-associated complex in spliceosome activationQ27938109
The spliceosome: design principles of a dynamic RNP machineQ28131809
Spliceosome assembly in yeastQ28285661
Single-molecule enzymatic dynamicsQ28290271
Allosteric cascade of spliceosome activationQ29615086
Conformational dynamics of single pre-mRNA molecules during in vitro splicingQ30053690
Biased Brownian ratcheting leads to pre-mRNA remodeling and capture prior to first-step splicingQ30559916
Viewing dynamic assembly of molecular complexes by multi-wavelength single-molecule fluorescenceQ33243370
Visualizing the splicing of single pre-mRNA molecules in whole cell extractQ33306330
Do-it-yourself guide: how to use the modern single-molecule toolkitQ34782759
Single-molecule analysis of protein-free U2-U6 snRNAsQ35010846
The evolutionarily conserved core design of the catalytic activation step of the yeast spliceosomeQ35014709
Single molecule approaches for studying spliceosome assembly and catalysisQ35096990
Rapid isolation and single-molecule analysis of ribonucleoproteins from cell lysate by SNAP-SiMPullQ35534924
Spliceosome assembly in the absence of stable U4/U6 RNA pairingQ35534989
Kinetic schemes for post-synchronized single molecule dynamicsQ35843500
The spliceosome: a flexible, reversible macromolecular machineQ36429942
The interaction of Prp2 with a defined region of the intron is required for the first splicing reactionQ36435201
Single-molecule colocalization FRET evidence that spliceosome activation precedes stable approach of 5' splice site and branch siteQ36799092
The DEAH box ATPases Prp16 and Prp43 cooperate to proofread 5' splice site cleavage during pre-mRNA splicingQ37043127
Exon ligation is proofread by the DExD/H-box ATPase Prp22pQ37059609
The EF-G-like GTPase Snu114p regulates spliceosome dynamics mediated by Brr2p, a DExD/H box ATPaseQ37183473
A role for ubiquitin in the spliceosome assembly pathwayQ37332858
Sad1 counteracts Brr2-mediated dissociation of U4/U6.U5 in tri-snRNP homeostasisQ37546093
The function of the NineTeen Complex (NTC) in regulating spliceosome conformations and fidelity during pre-mRNA splicingQ37775679
Stable tri-snRNP integration is accompanied by a major structural rearrangement of the spliceosome that is dependent on Prp8 interaction with the 5' splice siteQ38296438
Development of SNAP-tag fluorogenic probes for wash-free fluorescence imaging.Q39028238
SLU7 and a novel activity, SSF1, act during the PRP16-dependent step of yeast pre-mRNA splicingQ40789125
Assembly and dynamics of the U4/U6 di-snRNP by single-molecule FRET.Q41184400
hnRNP L and hnRNP A1 induce extended U1 snRNA interactions with an exon to repress spliceosome assemblyQ41226829
An RNA switch at the 5' splice site requires ATP and the DEAD box protein Prp28pQ41607972
The NineTeen Complex (NTC) and NTC-associated proteins as targets for spliceosomal ATPase action during pre-mRNA splicingQ41878070
Alternative spliceosome assembly pathways revealed by single-molecule fluorescence microscopyQ41961878
Post-transcriptional modifications modulate conformational dynamics in human U2-U6 snRNA complexQ42243790
Ordered and dynamic assembly of single spliceosomesQ42726090
Stoichiometry of a regulatory splicing complex revealed by single-molecule analysesQ42730959
Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicingQ42914749
Electrophoretic separation of complexes involved in the splicing of precursors to mRNAsQ43517287
An early hierarchic role of U1 small nuclear ribonucleoprotein in spliceosome assembly.Q45985841
A mechanism to enhance mRNA splicing fidelity: the RNA-dependent ATPase Prp16 governs usage of a discard pathway for aberrant lariat intermediatesQ46565000
In vitro assembly of yeast U6 snRNP: a functional assayQ46978210
Both catalytic steps of nuclear pre-mRNA splicing are reversibleQ81534595
P4510describes a project that usesImageQuantQ112270642
P407language of work or nameEnglishQ1860
P921main subjectspliceosomeQ915868
P577publication date2016-05-31
P1433published ineLifeQ2000008
P1476titleSingle molecule analysis reveals reversible and irreversible steps during spliceosome activation
P478volume5

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cites work (P2860)
Q41510811A new role for FBP21 as regulator of Brr2 helicase activity
Q38646470Dual RNA Processing Roles of Pat1b via Cytoplasmic Lsm1-7 and Nuclear Lsm2-8 Complexes
Q38614549Dynamics and consequences of spliceosome E complex formation.
Q38993788Functions and regulation of the Brr2 RNA helicase during splicing
Q39108397Genetics and biochemistry remain essential in the structural era of the spliceosome
Q93047547H3K36 Methylation and the Chromodomain Protein Eaf3 Are Required for Proper Cotranscriptional Spliceosome Assembly
Q30360791Identification of a small molecule inhibitor that stalls splicing at an early step of spliceosome activation.
Q48178037Life under the Microscope: Single-Molecule Fluorescence Highlights the RNA World
Q38993908Quantifying the Assembly of Multicomponent Molecular Machines by Single-Molecule Total Internal Reflection Fluorescence Microscopy
Q50911751Radical probing of spliceosome assembly.
Q38647449Single-Molecule Analysis of Pre-mRNA Splicing with Colocalization Single-Molecule Spectroscopy (CoSMoS).
Q92602462Smu1 and RED are required for activation of spliceosomal B complexes assembled on short introns
Q55647209Splicing heterogeneity: separating signal from noise.
Q41597888Structure of a pre-catalytic spliceosome
Q48172115The life of U6 small nuclear RNA, from cradle to grave

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