Site-specific mutagenesis and domain substitutions in the loading module of the nystatin polyketide synthase, and their effects on nystatin biosynthesis in Streptomyces noursei.

scientific article published on 19 February 2003

Site-specific mutagenesis and domain substitutions in the loading module of the nystatin polyketide synthase, and their effects on nystatin biosynthesis in Streptomyces noursei. is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.1074/JBC.M212611200
P953full work available at URLhttp://www.jbc.org/article/S0021925819302893/pdf
P698PubMed publication ID12594224
P5875ResearchGate publication ID10894118

P7228access restriction statusopen accessQ232932
P50authorSergey B. ZotchevQ48131830
P2093author name stringTrond E Ellingsen
Trygve Brautaset
Sven E F Borgos
Havard Sletta
P2860cites workDissection of malonyl-coenzyme A decarboxylation from polyketide formation in the reaction mechanism of a plant polyketide synthaseQ27621124
Mechanistic analysis of a type II polyketide synthase. Role of conserved residues in the beta-ketoacyl synthase-chain length factor heterodimerQ28145279
Alteration of the substrate specificity of a modular polyketide synthase acyltransferase domain through site-specific mutations.Q30329267
Identification of a gene cluster for antibacterial polyketide-derived antibiotic biosynthesis in the nystatin producer Streptomyces noursei ATCC 11455.Q30854131
A complex multienzyme system encoded by five polyketide synthase genes is involved in the biosynthesis of the 26-membered polyene macrolide pimaricin in Streptomyces natalensisQ30970107
One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solutionQ33846270
Amphotericin biosynthesis in Streptomyces nodosus: deductions from analysis of polyketide synthase and late genesQ33953578
Polyketide biosynthesis: a millennium reviewQ34362914
Quantitative analysis of loading and extender acyltransferases of modular polyketide synthasesQ40611944
Conversion of a beta-ketoacyl synthase to a malonyl decarboxylase by replacement of the active-site cysteine with glutamineQ41694678
Modular organization of genes required for complex polyketide biosynthesisQ42627871
4-[2-(2-amino-2-oxoethyl)-2,7-dihydroxy-4-oxochroman-5-yl]-3-hydroxybut-2-enoic acidQ43394722
Chain initiation on the soraphen-producing modular polyketide synthase from Sorangium cellulosumQ43832831
Engineering specificity of starter unit selection by the erythromycin-producing polyketide synthaseQ43936779
Analysis of Streptomyces avermitilis genes required for avermectin biosynthesis utilizing a novel integration vectorQ44047474
Spontaneous priming of a downstream module in 6-deoxyerythronolide B synthase leads to polyketide biosynthesisQ46190763
Biosynthesis of the polyene antifungal antibiotic nystatin in Streptomyces noursei ATCC 11455: analysis of the gene cluster and deduction of the biosynthetic pathway.Q47849112
Molecular cloning and analysis of a pleiotropic regulatory gene locus from the nystatin producer Streptomyces noursei ATCC11455.Q47931505
Hexaene derivatives of nystatin produced as a result of an induced rearrangement within the nysC polyketide synthase gene in S. noursei ATCC 11455.Q48311043
Engineering broader specificity into an antibiotic-producing polyketide synthaseQ48567903
A chain initiation factor common to both modular and aromatic polyketide synthasesQ60301879
Divergent sequence motifs correlated with the substrate specificity of (methyl)malonyl-CoA:acyl carrier protein transacylase domains in modular polyketide synthasesQ60301917
Glutamate-aspartate transaminase from microorganismsQ70123886
The loading domain of the erythromycin polyketide synthase is not essential for erythromycin biosynthesis in Saccharopolyspora erythraeaQ74285687
P433issue17
P407language of work or nameEnglishQ1860
P921main subjectnystatinQ251005
Streptomyces nourseiQ7623391
nystatin A1Q27292191
P6954online access statusopen accessQ232932
P304page(s)14913-14919
P577publication date2003-02-19
P1433published inJournal of Biological ChemistryQ867727
P1476titleSite-specific mutagenesis and domain substitutions in the loading module of the nystatin polyketide synthase, and their effects on nystatin biosynthesis in Streptomyces noursei
P478volume278

Reverse relations

cites work (P2860)
Q38091646Biosynthesis and pathway engineering of antifungal polyene macrolides in actinomycetes
Q30798676Butenyl-spinosyns, a natural example of genetic engineering of antibiotic biosynthetic genes
Q42153893Characterization of the P450 monooxygenase NysL, responsible for C-10 hydroxylation during biosynthesis of the polyene macrolide antibiotic nystatin in Streptomyces noursei.
Q40289243Chemical diversity of polyene macrolides produced by Streptomyces noursei ATCC 11455 and recombinant strain ERD44 with genetically altered polyketide synthase NysC.
Q34425098Crucial factor for increasing the conjugation frequency in Streptomyces netropsis SD-07 and other strains
Q47215208Engineered polyketides: Synergy between protein and host level engineering.
Q46052989Enzymology of the polyenes pimaricin and candicidin biosynthesis.
Q33280753Identification of novel genes involved in long-chain n-alkane degradation by Acinetobacter sp. strain DSM 17874.
Q40584907In vivo analysis of the regulatory genes in the nystatin biosynthetic gene cluster of Streptomyces noursei ATCC 11455 reveals their differential control over antibiotic biosynthesis.
Q38628127Initiation of polyene macrolide biosynthesis: interplay between polyketide synthase domains and modules as revealed via domain swapping, mutagenesis, and heterologous complementation
Q57970490New Alpiniamides From sp. IB2014/011-12 Assembled by an Unusual Hybrid Non-ribosomal Peptide Synthetase -AT Polyketide Synthase Enzyme
Q38628520New nystatin-related antifungal polyene macrolides with altered polyol region generated via biosynthetic engineering of Streptomyces noursei
Q39610313Nystatin biosynthesis and transport: nysH and nysG genes encoding a putative ABC transporter system in Streptomyces noursei ATCC 11455 are required for efficient conversion of 10-deoxynystatin to nystatin.
Q97425379Site directed mutagenesis as a precision tool to enable synthetic biology with engineered modular polyketide synthases

Search more.