One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution

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One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution is …
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scholarly articleQ13442814

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P819ADS bibcode1989PNAS...86.2172C
P356DOI10.1073/PNAS.86.7.2172
P932PMC publication ID286873
P698PubMed publication ID2648393
P5875ResearchGate publication ID20508614

P2093author name stringMiller RH
Chung CT
Niemela SL
P2860cites workNonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNAQ24562997
Studies on transformation of Escherichia coli with plasmidsQ27860598
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cellsQ34192132
Calcium-dependent bacteriophage DNA infectionQ34223120
A rapid and convenient method for the preparation and storage of competent bacterial cellsQ36001361
Factors affecting the transformation of Escherichia coli strain χ1776 by pBR322 plasmid DNAQ40978542
The genetic and biochemical basis of the transformability of Escherichia coli K12Q69221825
A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeastQ70314278
P433issue7
P407language of work or nameEnglishQ1860
P921main subjectEscherichia coliQ25419
P304page(s)2172-2175
P577publication date1989-04-01
P1433published inProceedings of the National Academy of Sciences of the United States of AmericaQ1146531
P1476titleOne-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution
P478volume86

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cites work (P2860)
Q34013396"NiCo Buster": engineering E. coli for fast and efficient capture of cobalt and nickel
Q109649907A Bacterial Cell-Based Assay To Study SARS-CoV-2 Protein-Protein Interactions
Q46602133A Colletotrichum gloeosporioides-induced esterase gene of nonclimacteric pepper (Capsicum annuum) fruit during ripening plays a role in resistance against fungal infection
Q38991415A Comparison of Two-Hybrid Approaches for Detecting Protein-Protein Interactions
Q33532971A DNA-binding peroxiredoxin of Coxiella burnetii is involved in countering oxidative stress during exponential-phase growth
Q60309733A New Distinct Clade for Iranian Isolates Based on the Polymerase, Nucleocapsid, and Non-structural Genes
Q92074734A New Synthetic Pathway for the Bioproduction of Glycolic Acid From Lignocellulosic Sugars Aimed at Maximal Carbon Conservation
Q33762242A cluster of genes involved in polysaccharide biosynthesis from Enterococcus faecalis OG1RF.
Q39928252A cluster of three genes (dapA, orf2, and dapB) of Brevibacterium lactofermentum encodes dihydrodipicolinate synthase, dihydrodipicolinate reductase, and a third polypeptide of unknown function
Q34384973A comparison and optimization of methods and factors affecting the transformation of Escherichia coli.
Q48074303A family of modular type mannuronan C-5-epimerase genes controls alginate structure in Azotobacter vinelandii.
Q28277443A fully decompressed synthetic bacteriophage øX174 genome assembled and archived in yeast
Q36105971A gene at 59 minutes on the Escherichia coli chromosome encodes a lipoprotein with unusual amino acid repeat sequences
Q36123964A gene encoding arginyl-tRNA synthetase is located in the upstream region of the lysA gene in Brevibacterium lactofermentum: regulation of argS-lysA cluster expression by arginine
Q34870712A genome-wide screen for identifying all regulators of a target gene
Q28268450A human brain L-3-hydroxyacyl-coenzyme A dehydrogenase is identical to an amyloid beta-peptide-binding protein involved in Alzheimer's disease
Q39784064A mutation in the D,D-carboxypeptidase penicillin-binding protein 3 of Streptococcus pneumoniae contributes to cefotaxime resistance of the laboratory mutant C604.
Q39480431A mutation that improves soluble recombinant hemoglobin accumulation in Escherichia coli in heme excess
Q39843061A new Azotobacter vinelandii mannuronan C-5-epimerase gene (algG) is part of an alg gene cluster physically organized in a manner similar to that in Pseudomonas aeruginosa
Q24685880A new gene involved in stationary-phase survival located at 59 minutes on the Escherichia coli chromosome
Q39680415A new modified ortho cleavage pathway of 3-chlorocatechol degradation by Rhodococcus opacus 1CP: genetic and biochemical evidence
Q54314519A new site-specific recombinase-mediated system for targeted multiple genomic deletions employing chimeric loxP and mrpS sites.
Q44796609A newly designed degenerate PCR primer based on pmoA gene for detection of nitrite-dependent anaerobic methane-oxidizing bacteria from different ecological niches
Q41710408A novel Pseudomonas aeruginosa strain with an oprD mutation in relation to a nosocomial respiratory infection outbreak in an intensive care unit
Q52544931A one-step method for genetic transformation of non-piliated Neisseria meningitidis.
Q33291019A portfolio of plasmids for identification and analysis of carotenoid pathway enzymes: Adonis aestivalis as a case study
Q33442093A probable aculeacin A acylase from the Ralstonia solanacearum GMI1000 is N-acyl-homoserine lactone acylase with quorum-quenching activity
Q35883764A rapid and highly efficient method for preparation of competent Escherichia coli cells
Q41898663A scaffold protein connects type IV pili with the Chp chemosensory system to mediate activation of virulence signaling in Pseudomonas aeruginosa
Q37073021A survey of methods for the purification of microbial flavohemoglobins
Q52842438A systematic approach to the amplified expression, functional characterization and purification of inositol transporters from Bacillus subtilis.
Q33336665A transcriptional roadmap to wood formation
Q41667348A two-component, multimeric endolysin encoded by a single gene
Q33787788A unique Coxiella burnetii lipoprotein involved in metal binding (LimB).
Q92886006A universal biomolecular integral feedback controller for robust perfect adaptation
Q34538174A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica.
Q38635176A versatile vector for mycobacterial protein production with a functional minimized acetamidase regulon.
Q37073099Acat1 knockdown gene therapy decreases amyloid-β in a mouse model of Alzheimer's disease
Q37125410Acetylation of the response regulator RcsB controls transcription from a small RNA promoter
Q37593060Active barnase variants with completely random hydrophobic cores
Q51556838Addicting diverse bacteria to a noncanonical amino acid.
Q24669592Adsorption of bacteriophage lambda on the LamB protein of Escherichia coli K-12: point mutations in gene J of lambda responsible for extended host range
Q35451950Advancements in the development of HIF-1α-activated protein switches for use in enzyme prodrug therapy
Q35077336Affinity probe capillary electrophoresis of insulin using a fluorescence-labeled recombinant Fab as an affinity probe
Q77425102Agonist-induced down-regulation of the beta2-adrenoceptor and its mRNA in human mononuclear leukocytes
Q37099990AhR/Arnt:XRE interaction: turning false negatives into true positives in the modified yeast one-hybrid assay
Q35599447AinS and a new family of autoinducer synthesis proteins
Q29618092AlkB-mediated oxidative demethylation reverses DNA damage in Escherichia coli
Q35841602Alteration of the Donor/Acceptor Spectrum of the (S)-Amine Transaminase from Vibrio fluvialis.
Q40607687Altering the holoenzyme composition and substrate specificity of protein phosphatase 2A.
Q90577327Alternative splice variants of rhomboid proteins: In silico analysis of database entries for select model organisms and validation of functional potential
Q35600395Amplification of a novel gene, sanA, abolishes a vancomycin-sensitive defect in Escherichia coli
Q47867376An Efficient Transformation Method for the Bioplastic-Producing "Knallgas" Bacterium Ralstonia eutropha H16.
Q47438884An acetylatable lysine controls CRP function in E. coli.
Q34410800An elongated spine of buried core residues necessary for in vivo folding of the parallel beta-helix of P22 tailspike adhesin
Q44485770An engineered chorismate mutase with allosteric regulation
Q35965155An intersubunit contact stimulating transcription initiation by E coli RNA polymerase: interaction of the alpha C-terminal domain and sigma region 4.
Q24549039Anaerobic toluene catabolism of Thauera aromatica: the bbs operon codes for enzymes of beta oxidation of the intermediate benzylsuccinate
Q39915375Analysis and expression of the thrC gene of Brevibacterium lactofermentum and characterization of the encoded threonine synthase.
Q28343870Analysis of a gene cluster of Enterococcus faecalis involved in polysaccharide biosynthesis
Q42909470Analysis of the mycosamine biosynthesis and attachment genes in the nystatin biosynthetic gene cluster of Streptomyces noursei ATCC 11455.
Q44621506Analytical strategies for improving the robustness and reproducibility of bioluminescent microbial bioreporters
Q33988236Application of a propionyl coenzyme A synthetase for poly(3-hydroxypropionate-co-3-hydroxybutyrate) accumulation in recombinant Escherichia coli
Q46768752Application of sequential integration for metabolic engineering of 1,2-propanediol production in yeast
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Q30678093Atypical genetic locus associated with constitutive production of enterocin B by Enterococcus faecium BFE 900.
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Q33518010Biochemical evidence for the tyrosine involvement in cationic intermediate stabilization in mouse beta-carotene 15, 15'-monooxygenase
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Q54339905Biosynthesis of iminodiacetic acid from iminodiacetonitrile by immobilized recombinant Escherichia coli harboring nitrilase.
Q91671413CRIMoClo plasmids for modular assembly and orthogonal chromosomal integration of synthetic circuits in Escherichia coli
Q43097385CTAB-mediated, single-step preparation of competent Escherichia coli, Bifidobacterium sp. and Kluyveromyces lactis cells.
Q47617857CW-EPR Spectroscopy and Site-Directed Spin Labeling to Study the Structural Dynamics of Ion Channels
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Q61443077Caspase-4 disaggregates lipopolysaccharide micelles via LPS-CARD interaction
Q33326607Cell death by SecTRAPs: thioredoxin reductase as a prooxidant killer of cells
Q55000827Cell-free prediction of protein expression costs for growing cells.
Q33809149Cell-to-cell transformation in Escherichia coli: a novel type of natural transformation involving cell-derived DNA and a putative promoting pheromone
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Q44103571Characterization and application of a newly synthesized 2-deoxyribose-5-phosphate aldolase
Q45964620Characterization and potential application in mercury bioremediation of highly mercury-resistant marine bacterium Bacillus thuringiensis PW-05.
Q39844458Characterization and regulation of the gene encoding nitrite reductase in Rhodobacter sphaeroides 2.4.3.
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Q39564777Characterization of a protocatechuate catabolic gene cluster from Rhodococcus opacus 1CP: evidence for a merged enzyme with 4-carboxymuconolactone-decarboxylating and 3-oxoadipate enol-lactone-hydrolyzing activity
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Q24681575Choline oxidase, a catabolic enzyme in Arthrobacter pascens, facilitates adaptation to osmotic stress in Escherichia coli
Q56936071Chorismate Mutase-Prephenate Dehydratase fromEscherichia coli
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Q49025389Clonetegration Using OSIP Plasmids: One-Step DNA Assembly and Site-Specific Genomic Integration in Bacteria.
Q35117319Cloning and characterization of a 3-N-aminoglycoside acetyltransferase gene, aac(3)-Ib, from Pseudomonas aeruginosa
Q35628642Cloning and characterization of argR, a gene that participates in regulation of arginine biosynthesis and catabolism in Pseudomonas aeruginosa PAO1
Q43614970Cloning and characterization of rac-like cDNAs from Arabidopsis thaliana.
Q38977507Cloning and expression of Neospora caninum dense-granule 7 in E. coli
Q54605377Cloning and expression of a haloacid dehalogenase fromPseudomonas sp. strain 19 S
Q36750715Cloning and expression of an Azotobacter vinelandii mannuronan C-5-epimerase gene
Q39547101Cloning and expression of three new Aazotobacter vinelandii genes closely related to a previously described gene family encoding mannuronan C-5-epimerases.
Q39916575Cloning and sequencing of a serine proteinase gene from a thermophilic Bacillus species and its expression in Escherichia coli.
Q42685285Cloning and sequencing of an endo-beta-1,4-glucanase gene mcenA from Micromonospora cellulolyticum 86W-16.
Q34540598Cloning of a genetic determinant from Clostridium difficile involved in adherence to tissue culture cells and mucus
Q34029501Cloning of genes encoding extracellular metalloproteases from Erwinia chrysanthemi EC16.
Q34012089Cloning of the gene encoding a novel thermostable alpha-galactosidase from Thermus brockianus ITI360.
Q43150998Cloning of the papaya chromoplast-specific lycopene beta-cyclase, CpCYC-b, controlling fruit flesh color reveals conserved microsynteny and a recombination hot spot
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Q44068913Functional characterization and application of a tightly regulated MekR/P mekA expression system in Escherichia coli and Pseudomonas putida
Q91608377Functional characterization of partial recombinant goat conglutinin: Its role as innate immunity marker and use as antigen in sandwich ELISA
Q54514373Functional complementation of E. coli secD and secG mutants by Helicobacter pylori homologues.
Q40398756Functional difference between the two oppositely oriented priming signals essential for the initiation of the broad host-range plasmid RSF1010 DNA replication
Q37362075Functional division and reconstruction of a plasmid replication origin: molecular dissection of the oriV of the broad-host-range plasmid RSF1010
Q98733622Functionally uncoupled transcription-translation in Bacillus subtilis
Q43595422Fused p47phox and p67phox truncations efficiently reconstitute NADPH oxidase with higher activity and stability than the individual components
Q33506938Gene cloning, protein characterization, and alteration of product selectivity for the trehalulose hydrolase and trehalulose synthase from "Pseudomonas mesoacidophila" MX-45.
Q48874047Generation of a double-fluorescent double-selectable Cre/loxP indicator vector for monitoring of intracellular recombination events.
Q39839360Generation of auxotrophic mutants of Enterococcus faecalis
Q36766774Generation of restriction map of Enterococcus faecalis OG1 and investigation of growth requirements and regions encoding biosynthetic function
Q42928887Genes from Cellvibrio mixtus encoding beta-1,3 endoglucanase
Q30928395Genes involved in anaerobic metabolism of phenol in the bacterium Thauera aromatica
Q33989043Genetic diversity among 3-chloroaniline- and aniline-degrading strains of the Comamonadaceae
Q54735797Genetic engineering of Pseudomonas putida KT2440 for rapid and high-yield production of vanillin from ferulic acid.
Q40286069Genetic relationship between soxRS and mar loci in promoting multiple antibiotic resistance in Escherichia coli
Q39155419Genomic differences in the background of different severity in juvenile-onset respiratory papillomatoses associated with human papillomavirus type 11.
Q53298175Genomic variations between colistin-susceptible and -resistant Pseudomonas aeruginosa clinical isolates and their effects on colistin resistance.
Q34147905Glutamate racemase is an endogenous DNA gyrase inhibitor
Q43597559HHV-8 ELISA based on a one-step affinity capture of biotinylated K8.1 antigen
Q30964833Hemin-binding surface protein from Bartonella quintana
Q73310673Heparin/endothelial cell growth supplement regulates matrix gene expression and prolongs life span of vascular smooth muscle cells through modulation of interleukin-1
Q53724475Heterologous expression and characterization of a 3-ketosteroid-∆1-dehydrogenase from Gordonia neofelifaecis and its utilization in the bioconversion of androst-4,9(11)-dien-3,17-dione.
Q40909354Heterologous expression and purification of active divercin V41, a class IIa bacteriocin encoded by a synthetic gene in Escherichia coli
Q54516264Heterologous gene expression in Lactococcus lactis; expression of the Azotobacter vinelandii algE6 gene product displaying mannuronan C-5 epimerase activity.
Q24548921Hfq is necessary for regulation by the untranslated RNA DsrA
Q73031714High copy number integration into the ribosomal DNA of the yeast Phaffia rhodozyma
Q39715440High efficiency 5 min transformation of Escherichia coli
Q37698471High-efficiency oligonucleotide-directed plasmid mutagenesis
Q36753939High-level resistance to cefotaxime and ceftazidime in Klebsiella pneumoniae isolates from Cleveland, Ohio
Q54443844High-throughput screening of activity and enantioselectivity of esterases.
Q50237169Highly efficient and easy protease-mediated protein purification.
Q34063861His-8 lowers the pKa of the essential Cys-12 residue of the ArsC arsenate reductase of plasmid R773.
Q64965648Homologous alignment cloning: a rapid, flexible and highly efficient general molecular cloning method.
Q58583944Horizontal Plasmid Transfer by Transformation in : Environmental Factors and Possible Mechanisms
Q35631832IF3-mediated suppression of a GUA initiation codon mutation in the recJ gene of Escherichia coli
Q51102923Identification and characterization of a thermostable and cobalt-dependent amidase from Burkholderia phytofirmans ZJB-15079 for efficient synthesis of (R)-3,3,3-trifluoro-2-hydroxy-2-methylpropionic acid
Q91321391Identification and characterization of an M cell marker in nasopharynx- and oropharynx-associated lymphoid tissue of sheep
Q42690852Identification and characterization of functional rat arylamine N-acetyltransferase 3: comparisons with rat arylamine N-acetyltransferases 1 and 2.
Q39838571Identification and characterization of sporulation gene spoVS from Bacillus subtilis
Q50317633Identification and characterization of the vanillin dehydrogenase YfmT in Bacillus subtilis 3NA.
Q39839103Identification and functional analysis of the transfer region of plasmid pMEA300 of the methylotrophic actinomycete Amycolatopsis methanolica.
Q30628326Identification of RNase T as a high-copy suppressor of the UV sensitivity associated with single-strand DNA exonuclease deficiency in Escherichia coli
Q47362079Identification of a Staphylococcus aureus Efflux Pump Regulator Using a DNA-Protein Affinity Technique
Q82979264Identification of a new molecular target of class IIa bacteriocins in Listeria monocytogenes EGDe
Q45218744Identification of a novel DNA element that promotes cell-to-cell transformation in Escherichia coli
Q36710680Identification of a novel prophage-like gene cluster actively expressed in both virulent and avirulent strains of Leptospira interrogans serovar Lai
Q77696832Identification of a potent DNase activity associated with RNase T of Escherichia coli
Q37395502Identification of a protein kinase multigene family of Dictyostelium discoideum: molecular cloning and expression of a cDNA encoding a developmentally regulated protein kinase
Q44638955Identification of a triad of arginine residues in the active site of the ArsC arsenate reductase of plasmid R773.
Q53476433Identification of aldehyde reductase catalyzing the terminal step for conversion of xylose to butanetriol in engineered Escherichia coli.
Q39568776Identification of conserved, RpoS-dependent stationary-phase genes of Escherichia coli.
Q48015362Identification of factors impeding the production of a single-chain antibody fragment in Escherichia coliby comparing in vivo and in vitro expression
Q45776699Identification of human T cell leukemia virus type IIb infection in the Wayu, an aboriginal population of Colombia.
Q41582828Identification of human type D retrovirus as a contaminant in a neuroblastoma cell line
Q39640528Identification of quinoide redox mediators that are formed during the degradation of naphthalene-2-sulfonate by Sphingomonas xenophaga BN6.
Q36678267Identification of rfbA, involved in B-band lipopolysaccharide biosynthesis in Pseudomonas aeruginosa serotype O5
Q34328286Identification of the genes involved in Riemerella anatipestifer biofilm formation by random transposon mutagenesis
Q43867532Identification of the structural genes for N-acetylmuramoyl-L-alanine amidase and its modifier in Bacillus subtilis 168: inactivation of these genes by insertional mutagenesis has no effect on growth or cell separation
Q104064529Identification of two fnr genes and characterisation of their role in the anaerobic switch in Sphingopyxis granuli strain TFA
Q42046990Identification, functional studies, and genomic comparisons of new members of the NnrR regulon in Rhodobacter sphaeroides
Q42219072Impact of heat shock step on bacterial transformation efficiency
Q34813620Improved method for high-efficiency electrotransformation of Escherichia coli with the large BAC plasmids
Q38298296Improvement and characterization of a hyperthermophilic glucose isomerase from Thermoanaerobacter ethanolicus and its application in production of high fructose corn syrup.
Q46937472Improvement of astaxanthin production by a newly isolated Phaffia rhodozyma mutant with low-energy ion beam implantation.
Q54450086Improvement of enterocin P purification process.
Q42575144In vitro characterization of an enzymatic redox cascade composed of an alcohol dehydrogenase, an enoate reductases and a Baeyer-Villiger monooxygenase
Q42090733In vivo and in vitro processing of the Bacillus subtilis transcript coding for glutamyl-tRNA synthetase, serine acetyltransferase, and cysteinyl-tRNA synthetase
Q54253136In vivo phosphorylation of a peptide tag for protein purification.
Q33607645Inactivation of the streptococcal erythrogenic toxin B gene (speB) in Streptococcus pyogenes
Q50184951Induction and cleavage of Salmonella typhimurium UmuD protein
Q34582287Ingestion of bacterially expressed double-stranded RNA inhibits gene expression in planarians.
Q28364291Inhibition of Escherichia coli CTP synthase by glutamate gamma-semialdehyde and the role of the allosteric effector GTP in glutamine hydrolysis
Q36234801Inhibition of acetyl phosphate-dependent transcription by an acetylatable lysine on RNA polymerase
Q50528595Inhibition of gene expression in Escherichia coli under hypergravity
Q35603200Inner core biosynthesis of lipooligosaccharide (LOS) in Neisseria meningitidis serogroup B: identification and role in LOS assembly of the alpha1,2 N-acetylglucosamine transferase (RfaK).
Q36709442Insights into the Function of a Second, Nonclassical Ahp Peroxidase, AhpA, in Oxidative Stress Resistance in Bacillus subtilis
Q34810448Interaction of the initiator protein of an IncB plasmid with its origin of DNA replication
Q39654505Involvement of PhoP-PhoS homologs in Enterococcus faecalis virulence
Q36155271Isolation and characterization of a new class of cytochrome d terminal oxidase mutants of Escherichia coli
Q48277054Isolation and characterization of a novel Bacillus strain from coffee phyllosphere showing antifungal activity
Q48029472Isolation and characterization of pyrene and benzo[a]pyrene-degrading Klebsiella pneumonia PL1 and its potential use in bioremediation
Q35625657Isolation and characterization of rcsB mutations that affect colanic acid capsule synthesis in Escherichia coli K-12
Q70673862Isolation of temperature-sensitive aminoacyl-tRNA synthetase mutants from an Escherichia coli strain harboring the pemK plasmid
Q35611849Isolation, characterization, and expression of the Corynebacterium glutamicum betP gene, encoding the transport system for the compatible solute glycine betaine
Q39858574Isolation, characterization, and sequence analysis of cryptic plasmids from Acinetobacter calcoaceticus and their use in the construction of Escherichia coli shuttle plasmids
Q42291208Ketoacylsynthase Domains of a Polyunsaturated Fatty Acid Synthase in Thraustochytrium sp. Strain ATCC 26185 Can Effectively Function as Stand-Alone Enzymes in Escherichia coli
Q50586384Lactobacillus futsaii CS3, a New GABA-Producing Strain Isolated from Thai Fermented Shrimp (Kung-Som).
Q36222809Large-scale reduction of the Bacillus subtilis genome: consequences for the transcriptional network, resource allocation, and metabolism
Q47423883Large-scale synthesis of tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate by a stereoselective carbonyl reductase with high substrate concentration and product yield
Q67729416Leftward ribosome frameshifting at a hungry codon
Q37597387Life history implications of rRNA gene copy number in Escherichia coli
Q34435846Ligand interactions of the ArsC arsenate reductase
Q38297492Ligand-mediated changes in the tryptophan synthase indole tunnel probed by nile red fluorescence with wild type, mutant, and chemically modified enzymes
Q58799783Localization and functional characterization of the pathogenesis-related proteins Rbe1p and Rbt4p in Candida albicans
Q38762268Measuring Caenorhabditis elegans life span in 96 well microtiter plates
Q34192636Mechanism of positive regulation by DsrA and RprA small noncoding RNAs: pairing increases translation and protects rpoS mRNA from degradation
Q35530116Membrane stresses induced by overproduction of free fatty acids in Escherichia coli
Q24650701Mental retardation linked to mutations in the HSD17B10 gene interfering with neurosteroid and isoleucine metabolism
Q93183439Metal Reduction and Protein Secretion Genes Required for Iodate Reduction by Shewanella oneidensis
Q41838123Metal- and DNA-binding properties and mutational analysis of the transcription activating factor, B, of coliphage 186: a prokaryotic C4 zinc-finger protein
Q71641190Methods and reagents. preparing ultra-competent Escherichia coli
Q36223848Microbial Community Composition Impacts Pathogen Iron Availability during Polymicrobial Infection
Q38264064Modern and simple construction of plasmid: saving time and cost
Q41965008Molecular Characterization of Phylogenetically Distinct Isolates of Grapevine fanleaf virus from Iran Based on 2A(HP) Gene
Q41674085Molecular Cloning, Expression and Peroxidase Conjugation of Staphylococcus aureus Protein A.
Q31123704Molecular Determinants of Mutant Phenotypes, Inferred from Saturation Mutagenesis Data
Q33744187Molecular assessment of microbiota structure and dynamics along mixed olive oil and winery wastewaters biotreatment.
Q29346828Molecular characterization and regulation of an operon encoding a system for transport of arginine and ornithine and the ArgR regulatory protein in Pseudomonas aeruginosa
Q30735020Molecular cloning and analysis of a yeast protein phosphatase with an unusual amino-terminal region
Q31010966Molecular cloning and characterization of a cDNA encoding soybean nodule IMP dehydrogenase
Q36636839Molecular cloning and characterization of an immunosuppressive and weakly oncogenic variant of Friend murine leukemia virus, FIS-2
Q42663204Molecular cloning and characterization of beta-defensin cDNA expressed in distal ileum of buffalo (Bubalus bubalis).
Q39934954Molecular cloning and physical mapping of the otsBA genes, which encode the osmoregulatory trehalose pathway of Escherichia coli: evidence that transcription is activated by katF (AppR)
Q48075744Molecular cloning of the hom-thrC-thrB cluster from Bacillus sp. ULM1: expression of the thrC gene in Escherichia coli and corynebacteria, and evolutionary relationships of the threonine genes
Q42732972Molecular cloning, expression and enzymatic assay of pteridine reductase 1 from Iranian lizard Leishmania.
Q48037109Molecular cloning, expression in Escherichia coli, and characterization of a novel L-3-hydroxyacyl coenzyme A dehydrogenase from pig liver
Q28575493Molecular cloning, modeling, and localization of rat type 10 17beta-hydroxysteroid dehydrogenase
Q35470601Monitoring methionine sulfoxide with stereospecific mechanism-based fluorescent sensors
Q36945984Monoclonal antibodies define genus-specific, species-specific, and cross-reactive epitopes of the chlamydial 60-kilodalton heat shock protein (hsp60): specific immunodetection and purification of chlamydial hsp60
Q33918072Monoclonal antibodies to CNA, a collagen-binding microbial surface component recognizing adhesive matrix molecules, detach Staphylococcus aureus from a collagen substrate
Q54315902Monounsaturated fatty acid aerobic synthesis in Bradyrhizobium TAL1000 peanut-nodulating is affected by temperature.
Q34628970More refined diversity of anammox bacteria recovered and distribution in different ecosystems.
Q40689252More than a hole: the holin lethal function may be required to fully sensitize bacteria to the lytic action of canonical endolysins.
Q56894122MreB polymers and curvature localization are enhanced by RodZ and predict E. coli's cylindrical uniformity
Q38669680Multilevel engineering of the upstream module of aromatic amino acid biosynthesis in Saccharomyces cerevisiae for high production of polymer and drug precursors.
Q48065198Multiple copies of MATE elements support autonomous plasmid replication in Aspergillus nidulans
Q40526728Mutagenesis of selC, the gene for the selenocysteine-inserting tRNA-species in E. coli: effects on in vivo function.
Q35946379Mutagenic potency of exocyclic DNA adducts: marked differences between Escherichia coli and simian kidney cells
Q39393704Mutagenicity testing in Salmonella typhimurium strains possessing both the His reversion and Ara forward mutation systems and different levels of classical nitroreductase or O-acetyltransferase activities
Q73305513Mutation of Thr445 and Ile500 of initiation factor 2 G-domain affects Escherichia coli growth rate at low temperature
Q36280989Mutational analysis of the active site of indoleglycerol phosphate synthase from Escherichia coli
Q36124476Mutational analysis of the bacteriophage P2 Ogr protein: truncation of the carboxy terminus
Q36784218Mutational analysis of the specific priming signal essential for DNA replication of the broad host-range plasmid RSF1010.
Q33701326Mutations in Bartonella bacilliformis gyrB confer resistance to coumermycin A1.
Q98771860Mutations in the TolC Periplasmic Domain Affect Substrate Specificity of the AcrAB-TolC Pump
Q70759379Mutations in the mutY gene of Escherichia coli enhance the frequency of targeted G:C-->T:a transversions induced by a single 8-oxoguanine residue in single-stranded DNA
Q36153905Mutations that affect activity of the Rhizobium meliloti trpE(G) promoter in Rhizobium meliloti and Escherichia coli
Q89965304NarAB Is an ABC-Type Transporter That Confers Resistance to the Polyether Ionophores Narasin, Salinomycin, and Maduramicin, but Not Monensin
Q39943924Natural transformation in Campylobacter species
Q36939039New biosynthetic step in the melanin pathway of Wangiella (Exophiala) dermatitidis: evidence for 2-acetyl-1,3,6,8-Tetrahydroxynaphthalene as a novel precursor
Q42048183New donor vector for generation of histidine-tagged fusion proteins using the Gateway Cloning System
Q42095420New insights into the QuikChange™ process guide the use of Phusion DNA polymerase for site-directed mutagenesis
Q36731181Ni(II) and Co(II) sensing by Escherichia coli RcnR.
Q43163267Nickel promotes biofilm formation by Escherichia coli K-12 strains that produce curli
Q39842767Nitric oxide, nitrite, and Fnr regulation of hmp (flavohemoglobin) gene expression in Escherichia coli K-12.
Q34549493Novel "superspreader" bacteriophages promote horizontal gene transfer by transformation
Q91933400Novel Bacteria-Immobilized Cellulose Acetate/Poly(ethylene oxide) Nanofibrous Membrane for Wastewater Treatment
Q33996611Novel genes of the sox gene cluster, mutagenesis of the flavoprotein SoxF, and evidence for a general sulfur-oxidizing system in Paracoccus pantotrophus GB17.
Q31008729Novel human testis-specific cDNA: molecular cloning, expression and immunobiological effects of the recombinant protein
Q29346843NtrC-dependent regulatory network for nitrogen assimilation in Pseudomonas putida
Q35776843Nucleotide sequence of a Porphyromonas gingivalis gene encoding a surface-associated glutamate dehydrogenase and construction of a glutamate dehydrogenase-deficient isogenic mutant
Q36754758Nucleotide sequence of the beta-lactamase gene from Enterococcus faecalis HH22 and its similarity to staphylococcal beta-lactamase genes
Q38327719Nucleotide sequences of genes encoding penicillin-binding proteins from Streptococcus pneumoniae and Streptococcus oralis with high homology to Escherichia coli penicillin-binding proteins 1a and 1b
Q33243022O-Acetylation of the terminal N-acetylglucosamine of the lipooligosaccharide inner core in Neisseria meningitidis. Influence on inner core structure and assembly.
Q33735963Oligohistidine tag mutagenesis of the lambda holin gene
Q33314657Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations
Q51529801Osmo-sensing by N- and C-terminal extensions of the glycine betaine uptake system BetP of Corynebacterium glutamicum.
Q90178807Osmolytes ameliorate the effects of stress in the absence of the heat shock protein Hsp104 in Saccharomyces cerevisiae
Q41810965Osteopontin is a tumor autoantigen in prostate cancer patients.
Q50060158Overexpression of Recombinant Selenoproteins in E. coli
Q36782635Overexpression of the MarA positive regulator is sufficient to confer multiple antibiotic resistance in Escherichia coli
Q41825705Overproduction of the pyruvate dehydrogenase multienzyme complex of Escherichia coli and site-directed substitutions in the E1p and E2p subunits
Q34532804PFunkel: efficient, expansive, user-defined mutagenesis.
Q64065120ParAlleL: A Novel Population-Based Approach to Biological Logic Gates
Q43499309Parallel origins of duplications and the formation of pseudogenes in mitochondrial DNA from parthenogenetic lizards (Heteronotia binoei; Gekkonidae).
Q39845672Paraquat regulation of hmp (flavohemoglobin) gene expression in Escherichia coli K-12 is SoxRS independent but modulated by sigma S.
Q37440287Partitioning of RNA polymerase activity in live Escherichia coli from analysis of single-molecule diffusive trajectories.
Q34325596Patterns of evolutionary conservation of essential genes correlate with their compensability
Q39764909Phage SPP1 reversible adsorption to Bacillus subtilis cell wall teichoic acids accelerates virus recognition of membrane receptor YueB.
Q54677071PhoA gene fusions in Legionella pneumophila generated in vivo using a new transposon, MudphoA
Q37328331Phosducin is a ubiquitous G-protein regulator.
Q46487770Phospholipids as determinants of membrane protein topology. Phosphatidylethanolamine is required for the proper topological organization of the gamma-aminobutyric acid permease (GabP) of Escherichia coli
Q39843636Phosphorylation-independent bacterial chemoresponses correlate with changes in the cytoplasmic level of fumarate
Q39662539Physiological roles for two periplasmic nitrate reductases in Rhodobacter sphaeroides 2.4.3 (ATCC 17025)
Q100415732Piperacillin/tazobactam resistance in a clinical isolate of Escherichia coli due to IS26-mediated amplification of blaTEM-1B
Q34462254Polar localization of Escherichia coli chemoreceptors requires an intact Tol-Pal complex
Q48076410Polymorphism of the thymidylate synthase gene of Pneumocystis carinii from different host species.
Q39476019Population genetic analysis of grapevine fanleaf virus
Q38877547Practical guide for dynamic monitoring of protein oxidation using genetically encoded ratiometric fluorescent biosensors of methionine sulfoxide.
Q102075275Predictive design of sigma factor-specific promoters
Q28492603Prevalence of gca, a gene involved in synthesis of A-band common antigen polysaccharide in Pseudomonas aeruginosa
Q74536378Primary structure of cold-adapted alkaline phosphatase from a Vibrio sp. as deduced from the nucleotide gene sequence
Q40483902Probing the function of the two holin-like proteins of bacteriophage SPP1.
Q43071817Production of 3-nitrosoindole derivatives by Escherichia coli during anaerobic growth
Q90240592Production of Human β-Actin Using a Bacterial Expression System with a Cold Shock Vector
Q37312843Production of Polyclonal Antibody against Grapevine fanleaf virus Movement Protein Expressed in Escherichia coli
Q39492521Production of recombinant alpha-galactosidases in Thermus thermophilus
Q39140344Prokaryotic expression of ovis aries conglutinin encoding neck and carbohydrate recognition domain and its functional characterization
Q36112988Propagation of pSC101 plasmids defective in binding of integration host factor
Q42684891Protein acetylation dynamics in response to carbon overflow in Escherichia coli
Q114371271Protein disulfide isomerase and heat shock cognate protein 70 interactions with rotavirus structural proteins using their purified recombinant versions
Q34586128Protein engineering with biosynthesized libraries from Bordetella bronchiseptica bacteriophage
Q24309231Protocadherins: a large family of cadherin-related molecules in central nervous system
Q33730410Purification and biochemical characterization of the lambda holin.
Q44697196Purification and characterization of 2'aminobiphenyl-2,3-diol 1,2-dioxygenase from Pseudomonas sp. LD2.
Q28492637Purification and characterization of an arginine regulatory protein, ArgR, from Pseudomonas aeruginosa and its interactions with the control regions for the car, argF, and aru operons
Q42690954Purification and characterization of an isoaspartyl dipeptidase from Escherichia coli
Q45855695Purification and characterization of recombinant mouse and herpes simplex virus ribonucleotide reductase R2 subunit
Q35131579Quinolone resistance locus nfxD of Escherichia coli is a mutant allele of the parE gene encoding a subunit of topoisomerase IV.
Q33914206RNase P cleaves transient structures in some riboswitches
Q37417013Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly
Q37173130Rational elicitation of cold-sensitive phenotypes.
Q33883304Reactivity of glutaredoxins 1, 2, and 3 from Escherichia coli shows that glutaredoxin 2 is the primary hydrogen donor to ArsC-catalyzed arsenate reduction.
Q37074672Receptor polymorphism restricts contact-dependent growth inhibition to members of the same species
Q21195735Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007
Q54625080Recombination between repeats in Escherichia coli by a recA-independent, proximity-sensitive mechanism.
Q70823251Recombinational stability of replicating plasmids in Aspergillus nidulans during transformation, vegetative growth and sexual reproduction
Q42689738Regulation of R100 conjugation requires traM in cis to traJ.
Q33286507Regulation of carbon and nitrogen utilization by CbrAB and NtrBC two-component systems in Pseudomonas aeruginosa
Q24644864Regulation of clpQ⁺Y⁺ (hslV⁺U⁺) gene expression in Escherichia coli
Q35590911Regulation of coronafacoyl phytotoxin production by the PAS-LuxR family regulator CfaR in the common scab pathogen Streptomyces scabies
Q36122133Regulation of the Salmonella typhimurium metF gene by the MetR protein
Q36123722Regulation of the gltBDF operon of Escherichia coli: how is a leucine-insensitive operon regulated by the leucine-responsive regulatory protein?
Q34386450Regulation of the operon responsible for broad-spectrum mercury resistance in Streptomyces lividans 1326.
Q42648356Regulation, unique gene organization, and unusual primary structure of carbon fixation genes from a marine phycoerythrin-containing cyanobacterium
Q47315852Relationship between plasmid size and shock wave-mediated bacterial transformation
Q41908200Repression of flagellar genes in exponential phase by CsgD and CpxR, two crucial modulators of Escherichia coli biofilm formation
Q36828221Requirements for perpendicular helix pairing
Q38348152Residues in the alpha helix 7 of the bacterial maltose binding protein which are important in interactions with the Mal FGK2 complex
Q57295247Restoration of biofuel production levels and increased tolerance under ionic liquid stress is enabled by a mutation in the essential Escherichia coli gene cydC
Q92525147Reverse Genetic Systems for Pseudomonas aeruginosa Leviphages
Q54142634Revised Mechanism and Improved Efficiency of the QuikChange Site-Directed Mutagenesis Method.
Q38631070Ribozyme stability, exon skipping, and a potential role for RNA helicase in group I intron splicing by Coxiella burnetii
Q54493170Role of MetR and PurR in the activation of glyA by CsgD in Escherichia coli K-12.
Q34351778Role of RepA and DnaA proteins in the opening of the origin of DNA replication of an IncB plasmid
Q36116203Role of conserved aspartates in the ArsA ATPase
Q37171752Role of signature lysines in the deviant walker a motifs of the ArsA ATPase.
Q34202571Role of tRNA orthogonality in an expanded genetic code
Q54447033Role of the capsid protein VP4 in the plasma-dependent enhancement of the Coxsackievirus B4E2-infection of human peripheral blood cells.
Q47981458Roles of Transcriptional and Translational Control Mechanisms in Regulation of Ribosomal Protein Synthesis in Escherichia coli
Q33966292S elements: a family of Tc1-like transposons in the genome of Drosophila melanogaster
Q54660904SSV1-encoded site-specific recombination system in Sulfolobus shibatae.
Q52481330Saccharomyces cerevisiaegene SIT4 is involved in the control of glycogen metabolism
Q34796668Screening and Improving the Recombinant Nitrilases and Application in Biotransformation of Iminodiacetonitrile to Iminodiacetic Acid.
Q36015187Screening of an Escherichia coli promoter library for a phenylalanine biosensor
Q84890883Screening, cultivation, and biocatalytic performance of Rhodococcus boritolerans FW815 with strong 2,2-dimethylcyclopropanecarbonitrile hydratase activity
Q31918061Screening, nucleotide sequence, and biochemical characterization of an esterase from Pseudomonas fluorescens with high activity towards lactones
Q30330507Secretory production of recombinant human C-reactive protein in Escherichia coli, capable of binding with phosphorylcholine, and its characterization.
Q38628467Self-inducible Bacillus subtilis expression system for reliable and inexpensive protein production by high-cell-density fermentation
Q33213686Sensitive assay for laboratory evolution of hydroxylases toward aromatic and heterocyclic compounds.
Q40695874Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution
Q41855735Sequence-based appraisal of the genes encoding neck and carbohydrate recognition domain of conglutinin in blackbuck (Antilope cervicapra) and goat (Capra hircus).
Q34439737Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases.
Q42083492Sequencing and heterologous expression of an epimerase and two lyases from iminodisuccinate-degrading bacteria
Q39931888Sequencing, chromosomal inactivation, and functional expression in Escherichia coli of ppsR, a gene which represses carotenoid and bacteriochlorophyll synthesis in Rhodobacter sphaeroides
Q33281445SigmaE regulates and is regulated by a small RNA in Escherichia coli
Q28200768Signal transduction cascade for regulation of RpoS: temperature regulation of DsrA
Q30501142Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjD
Q41777210Single-strand DNA-specific exonucleases in Escherichia coli. Roles in repair and mutation avoidance.
Q55051342Site-directed Mutagenesis of Key Residues Unveiled a Novel Allosteric Site on Human Adenosine Kinase for Pyrrolobenzoxa(thia)zepinone Non-Nucleoside Inhibitors.
Q44320741Site-specific mutagenesis and domain substitutions in the loading module of the nystatin polyketide synthase, and their effects on nystatin biosynthesis in Streptomyces noursei.
Q39493764Slipped misalignment mechanisms of deletion formation: in vivo susceptibility to nucleases
Q36709429Small RNA Regulation of TolC, the Outer Membrane Component of Bacterial Multidrug Transporters
Q39840217Soft metal thiol chemistry is not involved in the transport of arsenite by the Ars pump
Q67524016Spatial proximity of Cys113, Cys172, and Cys422 in the metalloactivation domain of the ArsA ATPase
Q39566897Species-dependent phenotypes of replication-temperature-sensitive trfA mutants of plasmid RK2: a codon-neutral base substitution stimulates temperature sensitivity by leading to reduced levels of trfA expression.
Q27309068Specific RNA Interference in Caenorhabditis elegans by Ingested dsRNA Expressed in Bacillus subtilis
Q42330324Spot 42 sRNA regulates arabinose-inducible araBAD promoter activity by repressing synthesis of the high-affinity low-capacity arabinose transporter
Q37489429Stabilization of diverged tandem repeats by mismatch repair: evidence for deletion formation via a misaligned replication intermediate.
Q34329432Stabilization of perfect and imperfect tandem repeats by single-strand DNA exonucleases
Q36646366Strand-biased cytosine deamination at the replication fork causes cytosine to thymine mutations in Escherichia coli.
Q90212175Structural Changes in Stx1 Engineering Monoclonal Antibody Improves Its Functionality as Diagnostic Tool for a Rapid Latex Agglutination Test
Q40547739Structural and functional features of cis-acting sequences in the basic replicon of plasmid ColIb-P9
Q50113178Structural and mechanistic analysis of a β-glycoside phosphorylase identified by screening a metagenomic library
Q54453326Structure and function of the 3-carboxy-cis,cis-muconate lactonizing enzyme from the protocatechuate degradative pathway of Agrobacterium radiobacter S2.
Q42672286Structure and function of the genes involved in mannitol, arabitol and glucitol utilization from Pseudomonas fluorescens DSM50106.
Q40180958Structure and properties of chimeric small heat shock proteins containing yellow fluorescent protein attached to their C-terminal ends
Q36114525Structure and regulation of the Yersinia pestis yscBCDEF operon
Q31150306Structure to function prediction of hypothetical protein KPN_00953 (Ycbk) from Klebsiella pneumoniae MGH 78578 highlights possible role in cell wall metabolism
Q43862144Structure-function analysis of the ArsA ATPase: contribution of histidine residues.
Q37346935Substrate ambiguous enzymes within the Escherichia coli proteome offer different evolutionary solutions to the same problem
Q33719711Substrate specificity of and product formation by muconate cycloisomerases: an analysis of wild-type enzymes and engineered variants
Q38345201Subunit interactions in ABC transporters: aconserved sequence in hydrophobic membrane proteins of periplasmic permeases defines an important site of interaction with the ATPase subunits
Q48119949Successful completion of a semi-automated enzyme-free cloning method.
Q33965069Suppression of recJ exonuclease mutants of Escherichia coli by alterations in DNA helicases II (uvrD) and IV (helD)
Q36122603Suppression of recJ mutations of Escherichia coli by mutations in translation initiation factor IF3
Q39951286Suppression of the lethal effect of acidic-phospholipid deficiency by defective formation of the major outer membrane lipoprotein in Escherichia coli
Q43027905Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein.
Q41910052Synthesis, cloning and expression of a novel pre-miniproinsulin analogue gene in Escherichia coli
Q54443867Tagging recombinant proteins with a Sel-tag for purification, labeling with electrophilic compounds or radiolabeling with 11C.
Q40104894Targeted DNA methylation in human cells using engineered dCas9-methyltransferases.
Q39536285Targeted disruption of the kstD gene encoding a 3-ketosteroid delta(1)-dehydrogenase isoenzyme of Rhodococcus erythropolis strain SQ1.
Q28181441Temperature sensing by the dsrA promoter
Q35126802Tethering of spherical DOTAP liposome gold nanoparticles on cysteamine monolayer for sensitive label free electrochemical detection of DNA and transfection
Q74010157The 'wildtype' conformation of p53: epitope mapping using hybrid proteins
Q39495700The A modules of the Azotobacter vinelandii mannuronan-C-5-epimerase AlgE1 are sufficient for both epimerization and binding of Ca2+.
Q29346801The ArgR regulatory protein, a helper to the anaerobic regulator ANR during transcriptional activation of the arcD promoter in Pseudomonas aeruginosa
Q77545066The Azotobacter vinelandii mannuronan C-5-epimerase AlgE1 consists of two separate catalytic domains
Q54439907The Bacillus subtilis mannose regulator, ManR, a DNA-binding protein regulated by HPr and its cognate PTS transporter ManP.
Q41676477The Biofilm Inhibitor Carolacton Enters Gram-Negative Cells: Studies Using a TolC-Deficient Strain of Escherichia coli
Q46430695The DNA target determines the dimerization partner selected by bHLHZ-like hybrid proteins AhRJun and ArntFos.
Q37124857The Escherichia coli CydX protein is a member of the CydAB cytochrome bd oxidase complex and is required for cytochrome bd oxidase activity.
Q47708947The Ile(L229) → Met mutation impairs the quinone binding to the QB-pocket in reaction centers of Rhodobacter sphaeroides
Q37545684The MiaA tRNA modification enzyme is necessary for robust RpoS expression in Escherichia coli
Q38289389The N terminus of the Escherichia coli transcription activator MalT is the domain of interaction with MalY.
Q34316444The N-acetyltransferase RimJ responds to environmental stimuli to repress pap fimbrial transcription in Escherichia coli
Q39757026The Pseudomonas fluorescens AlgG protein, but not its mannuronan C-5-epimerase activity, is needed for alginate polymer formation.
Q34491677The SMC-like protein complex SbcCD enhances DNA polymerase IV-dependent spontaneous mutation in Escherichia coli
Q35620892The Salmonella typhimurium mar locus: molecular and genetic analyses and assessment of its role in virulence
Q28480433The YfiBNR signal transduction mechanism reveals novel targets for the evolution of persistent Pseudomonas aeruginosa in cystic fibrosis airways
Q24674895The ars operon of Escherichia coli confers arsenical and antimonial resistance
Q39573884The bHLH transcription factor CgbHLH001 is a potential interaction partner of CDPK in halophyte Chenopodium glaucum
Q36138188The carboxy-terminal 14 amino acids of phage lambda N protein are dispensable for transcription antitermination
Q43628882The catalytic activities of the bifunctional Azotobacter vinelandii mannuronan C-5-epimerase and alginate lyase AlgE7 probably originate from the same active site in the enzyme.
Q34893085The cost of antibiotic resistance depends on evolutionary history in Escherichia coli.
Q39485715The function of cytoplasmic flavin reductases in the reduction of azo dyes by bacteria
Q68004224The gene DIS2S1 is essential in Saccharomyces cerevisiae and is involved in glycogen phosphorylase activation
Q27934586The gene PPG encodes a novel yeast protein phosphatase involved in glycogen accumulation
Q48083067The gene of the N-acetylglucosaminidase, a Bacillus subtilis 168 cell wall hydrolase not involved in vegetative cell autolysis
Q35617430The glutamic acid residue at amino acid 261 of the alpha subunit is a determinant of the intrinsic efficiency of RNA polymerase at the metE core promoter in Escherichia coli
Q36812721The i6A37 tRNA modification is essential for proper decoding of UUX-Leucine codons during rpoS and iraP translation
Q28776848The ilvIH operon of Escherichia coli is positively regulated
Q52969912The interaction domains of the DnaA and DnaB replication proteins of Escherichia coli.
Q50524501The lambda holin accumulates beyond the lethal triggering concentration under hyperexpression conditions.
Q35576418The leucine-responsive regulatory protein of Escherichia coli negatively regulates transcription of ompC and micF and positively regulates translation of ompF.
Q32053918The mannitol utilization genes of Pseudomonas fluorescens are regulated by an activator: cloning, nucleotide sequence and expression of the mtlR gene
Q54080003The native virulence plasmid combination affects the segregational stability of a theta-replicating shuttle vector in Bacillus anthracis var. New Hampshire.
Q48068054The nucleotide sequence of HLA-B*2704 reveals a new amino acid substitution in exon 4 which is also present in HLA-B*2706.
Q36233266The pas8 mutant of Pichia pastoris exhibits the peroxisomal protein import deficiencies of Zellweger syndrome cells--the PAS8 protein binds to the COOH-terminal tripeptide peroxisomal targeting signal, and is a member of the TPR protein family
Q47686824The periplasmic transaminase PtaA of Pseudomonas fluorescens converts the glutamic acid residue at the pyoverdine fluorophore to α-ketoglutaric acid
Q39939554The phenotypes of temperature-sensitive mini-RK2 replicons carrying mutations in the replication control gene trfA are suppressed nonspecifically by intragenic cop mutations
Q34000839The rgg gene of Streptococcus pyogenes NZ131 positively influences extracellular SPE B production.
Q34378080The role of arsenic-thiol interactions in metalloregulation of the ars operon
Q54204804The role of temperature and bivalent ions in preparing competent Escherichia coli.
Q33902492The role of the eae gene of enterohemorrhagic Escherichia coli in intimate attachment in vitro and in a porcine model
Q24562141The small RNA, DsrA, is essential for the low temperature expression of RpoS during exponential growth in Escherichia coli
Q60301358The spermidine acetyltransferase SpeG regulates transcription of the small RNA rprA
Q28595522The synthetic xylulose-1 phosphate pathway increases production of glycolic acid from xylose-rich sugar mixtures
Q45849978The tyrosine recombinase MrpA and its target sequence: a mutational analysis of the recombination site mrpS resulting in a new left element/right element (LE/RE) deletion system
Q39679418Thermoadaptation of alpha-galactosidase AgaB1 in Thermus thermophilus
Q39536248Thermostabilization of proteins by diglycerol phosphate, a new compatible solute from the hyperthermophile Archaeoglobus fulgidus
Q36168750Tissue expression and genomic sequences of rat N-acetyltransferases rNat1, rNat2, rNat3, and Functional characterization of a novel rNat3*2 genetic variant
Q42957774Titration and conditional knockdown of the prfB gene in Escherichia coli: effects on growth and overproduction of the recombinant mammalian selenoprotein thioredoxin reductase.
Q34612485Toxicity and tolerance mechanisms for azidothymidine, a replication gap-promoting agent, in Escherichia coli
Q35407636Toxin-antitoxin systems in the mobile genome of Acidithiobacillus ferrooxidans
Q43953896Transcriptional analysis of the genetic elements involved in the lysogeny/lysis switch in the temperate lactococcal bacteriophage phiLC3, and identification of the Cro-like protein ORF76.
Q39847649Transcriptional regulation of the cydDC operon, encoding a heterodimeric ABC transporter required for assembly of cytochromes c and bd in Escherichia coli K-12: regulation by oxygen and alternative electron acceptors
Q41670055Transposon mutations in the 5' end of glnD, the gene for a nitrogen regulatory sensor, that suppress the osmosensitive phenotype caused by otsBA lesions in Escherichia coli
Q32060744Tumor protease-activated, pore-forming toxins from a combinatorial library.
Q36112479Two-stage induction of the soxRS (superoxide response) regulon of Escherichia coli
Q53789966Upscale production of a recombinant cyclodextrin glycosyltransferase from Paenibacillus macerans in Escherichia coli.
Q41714295Upscale production of ethyl (S)-4-chloro-3-hydroxybutanoate by using carbonyl reductase coupled with glucose dehydrogenase in aqueous-organic solvent system
Q35401490Urease-specific monoclonal antibodies prevent Helicobacter felis infection in mice
Q35687200Use of Polymerase Chain Reaction To Detect the Take-All Fungus, Gaeumannomyces graminis, in Infected Wheat Plants
Q92001963Use of a scaffold peptide in the biosynthesis of amino acid-derived natural products
Q77702919Using chromosomal lacIQ1 to control expression of genes on high-copy-number plasmids in Escherichia coli
Q42051670Using student-generated UV-induced Escherichia coli mutants in a directed inquiry undergraduate genetics laboratory
Q39717662Zn2+-sensing by the cyanobacterial metallothionein repressor SmtB: different motifs mediate metal-induced protein-DNA dissociation
Q31161300dcd (dCTP deaminase) gene of Escherichia coli: mapping, cloning, sequencing, and identification as a locus of suppressors of lethal dut (dUTPase) mutations
Q36105121holE, the gene coding for the theta subunit of DNA polymerase III of Escherichia coli: characterization of a holE mutant and comparison with a dnaQ (epsilon-subunit) mutant
Q35618784nfi, the gene for endonuclease V in Escherichia coli K-12.
Q37210961sRNA roles in regulating transcriptional regulators: Lrp and SoxS regulation by sRNAs

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