Improved detection of BRAF V600E using allele-specific PCR coupled with external and internal controllers

scientific article published on 23 October 2017

Improved detection of BRAF V600E using allele-specific PCR coupled with external and internal controllers is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1038/S41598-017-14140-2
P932PMC publication ID5653796
P698PubMed publication ID29061997

P2093author name stringQing Huang
Zhao Yang
Ning Su
Han-Qing Xu
Wei-Ling Fu
Dong Chen
Na Zhao
Kun Wei
Jun-Fu Huang
Guang-Jie Duan
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Oncogenic activation of the RAS/RAF signaling pathway impairs the response of metastatic colorectal cancers to anti-epidermal growth factor receptor antibody therapies.Q55043614
Allele specific Taqman-based real-time PCR assay to quantify circulating BRAFV600E mutated DNA in plasma of melanoma patientsQ57975214
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COLD-PCR-enhanced high-resolution melting enables rapid and selective identification of low-level unknown mutationsQ33689438
The Taguchi methodology as a statistical tool for biotechnological applications: a critical appraisalQ34758630
Blocking primers to enhance PCR amplification of rare sequences in mixed samples - a case study on prey DNA in Antarctic krill stomachsQ34797930
KRAS mutations detected by the amplification refractory mutation system-Scorpion assays strongly correlate with therapeutic effect of cetuximabQ35212649
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An improved allele-specific PCR primer design method for SNP marker analysis and its applicationQ36391841
Clinical relevance of KRAS mutation detection in metastatic colorectal cancer treated by Cetuximab plus chemotherapyQ36610301
KRAS and BRAF mutation analysis in colorectal adenocarcinoma specimens with a low percentage of tumor cells.Q36872978
Simple allele-discriminating PCR for cost-effective and rapid genotyping and mappingQ37111992
Biomarkers predicting clinical outcome of epidermal growth factor receptor-targeted therapy in metastatic colorectal cancerQ37378581
High-sensitivity PCR method for detecting BRAF V600E mutations in metastatic colorectal cancer using LNA/DNA chimeras to block wild-type allelesQ39026752
ARMS-PCR for detection of BRAF V600E hotspot mutation in comparison with Real-Time PCR-based techniquesQ39185287
Benign nodal nevi frequently harbor the activating V600E BRAF mutationQ40025064
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Multiplex picodroplet digital PCR to detect KRAS mutations in circulating DNA from the plasma of colorectal cancer patients.Q40130589
Sensitive detection of BRAF V600E mutation by Amplification Refractory Mutation System (ARMS)-PCR.Q42176497
Effect of low-frequency KRAS mutations on the response to anti-EGFR therapy in metastatic colorectal cancer.Q45376093
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P433issue1
P407language of work or nameEnglishQ1860
P304page(s)13817
P577publication date2017-10-23
P1433published inScientific ReportsQ2261792
P1476titleImproved detection of BRAF V600E using allele-specific PCR coupled with external and internal controllers
P478volume7

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cites work (P2860)
Q94481866Evaluation of the expression levels of BRAFV600E mRNA in primary tumors of thyroid cancer using an ultrasensitive mutation assay
Q64948273Sensitive detection of low-abundance in-frame deletions in EGFR exon 19 using novel wild-type blockers in real-time PCR.

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