Simple Methods and Rational Design for Enhancing Aptamer Sensitivity and Specificity.

scientific article published on 14 May 2018

Simple Methods and Rational Design for Enhancing Aptamer Sensitivity and Specificity. is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.3389/FMOLB.2018.00041
P932PMC publication ID5966647
P698PubMed publication ID29868605

P2093author name stringWilliam C Cho
John G Bruno
Tarun K Sharma
Priya Kalra
Abhijeet Dhiman
P2860cites workDevelopment of a fluorescent enzyme-linked DNA aptamer-magnetic bead sandwich assay and portable fluorometer for sensitive and rapid listeria detectionQ46798377
Disruption of liver development and coagulation pathway by ochratoxin A in embryonic zebrafishQ49597198
Computational modeling of peptide-aptamer binding.Q50986022
Aptamer-controlled reversible inhibition of gold nanozyme activity for pesticide sensing.Q51040368
Aptamer Cell-Based Selection: Overview and Advances.Q52544489
Sensitivity and selectivity on aptamer-based assay: the determination of tetracycline residue in bovine milk.Q55393941
Polymerase engineering: towards the encoded synthesis of unnatural biopolymersQ56897374
Aptamer Capturing of Enzymes on Magnetic Beads to Enhance Assay Specificity and SensitivityQ62507639
Utilization of the biotin/avidin system to amplify the sensitivity of the enzyme-linked immunosorbent assay (ELISA)Q71689200
Non-SELEX selection of aptamersQ82428995
Aptamer-mediated 'turn-off/turn-on' nanozyme activity of gold nanoparticles for kanamycin detectionQ85833005
Amplification, mutation, and sequencing of a six-letter synthetic genetic systemQ22162463
Automated 3D structure composition for large RNAsQ24622388
Selection and Biosensor Application of Aptamers for Small MoleculesQ26744467
Methods for Improving Aptamer Binding AffinityQ26752642
Unique motifs and hydrophobic interactions shape the binding of modified DNA ligands to protein targetsQ27675037
Structural basis for discriminatory recognition of Plasmodium lactate dehydrogenase by a DNA aptamerQ27679972
Structural basis of ligand discrimination by two related RNA aptamers resolved by NMR spectroscopyQ27732779
Aptamer-Mediated Targeted Delivery of Therapeutics: An UpdateQ28066827
Multivalent Aptamers: Versatile Tools for Diagnostic and Therapeutic ApplicationsQ28073119
Ochratoxin A: An overview on toxicity and carcinogenicity in animals and humansQ28281142
Aptamer-based multiplexed proteomic technology for biomarker discoveryQ28744329
Unlocking biomarker discovery: large scale application of aptamer proteomic technology for early detection of lung cancerQ28744369
In vitro selection using a dual RNA library that allows primerless selectionQ29036552
In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein A.Q30406029
Profiling of active thrombin in human blood by supramolecular complexesQ38335098
Analysis of aptamer sequence activity relationshipsQ38347807
Aptamers as a model for functional evaluation of LNA and 2'-amino LNA.Q38349317
Bivalent ligands with long nanometer-scale flexible linkersQ38357837
Array-based evolution of DNA aptamers allows modelling of an explicit sequence-fitness landscapeQ38358691
Predicting the Uncertain Future of Aptamer-Based Diagnostics and Therapeutics.Q38443249
3D Cell-SELEX: Development of RNA aptamers as molecular probes for PC-3 tumor cell line.Q38798663
Truncation and Mutation of a Transferrin Receptor Aptamer Enhances Binding Affinity.Q38833504
Aptamer Affinity Maturation by Resampling and Microarray Selection.Q39653640
Let's get specific: the relationship between specificity and affinityQ41651463
Evolution of aptamers with a new specificity and new secondary structures from an ATP aptamerQ42061051
Application of DNA Aptamers and Quantum Dots to Lateral Flow Test Strips for Detection of Foodborne Pathogens with Improved Sensitivity versus Colloidal GoldQ43030779
Aptamer-functionalized gold nanoparticles as probes in a dry-reagent strip biosensor for protein analysisQ43484075
Concentration, detection and discrimination of Bacillus anthracis spores in orange juice using aptamer based surface enhanced Raman spectroscopyQ44667223
Differentiating Siblings: The Case of Dopamine and NorepinephrineQ46087477
Determination of ochratoxin a with a DNA aptamerQ46265506
Recurrent RNA motifs as scaffolds for genetically encodable small-molecule biosensorsQ36250556
ABCs of DNA aptamer and related assay developmentQ36256140
An analysis of the association between prostate cancer risk loci, PSA levels, disease aggressiveness and disease-specific mortalityQ36286694
Generation of Aptamers from A Primer-Free Randomized ssDNA Library Using Magnetic-Assisted Rapid Aptamer SelectionQ36331455
Selection is more intelligent than design: improving the affinity of a bivalent ligand through directed evolutionQ36478067
1020Fold aptamer library amplification without gel purificationQ36697437
A general strategy for expanding polymerase function by droplet microfluidicsQ36768809
Direct and site-specific quantification of RNA 2'-O-methylation by PCR with an engineered DNA polymeraseQ36870545
Micromagnetic selection of aptamers in microfluidic channelsQ37087131
Evoking picomolar binding in RNA by a single phosphorodithioate linkage.Q37294897
Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureusQ37298613
Laboratory evolution of artificially expanded DNA gives redesignable aptamers that target the toxic form of anthrax protective antigenQ37523025
Proteomics analysis of cancer exosomes using a novel modified aptamer-based array (SOMAscan™) platform.Q37687417
Aptamer-based sandwich-type biosensorsQ37695007
Selection of DNA aptamers with two modified basesQ37711632
Preliminary Development of a DNA Aptamer-Magnetic Bead Capture Electrochemiluminescence Sandwich Assay for Brain Natriuretic PeptideQ37714328
High-content affinity-based proteomics: unlocking protein biomarker discoveryQ37809868
Sensitivity and specificity: twin goals of proteomics assays. Can they be combined?Q38097843
Aptamer-based biosensors for biomedical diagnosticsQ38204726
Towards XNA nanotechnology: new materials from synthetic genetic polymersQ38205549
Aptamers as targeted therapeutics: current potential and challengesQ38290290
Computational approach to analyze isolated ssDNA aptamers against angiotensin II.Q38292477
β-Conglutin dual aptamers binding distinct aptatopes.Q38295201
Aptamer Binding Studies Using MicroScale ThermophoresisQ38295400
Studying small molecule-aptamer interactions using MicroScale Thermophoresis (MST).Q38296668
Computational docking simulations of a DNA-aptamer for argininamide and related ligands.Q38299764
RNA aptamers as genetic control devices: the potential of riboswitches as synthetic elements for regulating gene expressionQ38301085
Magnetic-assisted rapid aptamer selection (MARAS) for generating high-affinity DNA aptamer using rotating magnetic fieldsQ38307210
Efficient isolation and elution of cellular proteins using aptamer-mediated protein precipitation assay.Q38307525
Beyond antibodies: new affinity reagents to unlock the proteomeQ38309923
Aptamer cocktails: enhancement of sensing signals compared to single use of aptamers for detection of bacteriaQ38310848
Affinity improvement of a VEGF aptamer by in silico maturation for a sensitive VEGF-detection system.Q38319303
Monitoring of plasma levels of activated protein C using a clinically applicable oligonucleotide-based enzyme capture assayQ38328800
DNA aptamer beacon assay for C-telopeptide and handheld fluorometer to monitor bone resorptionQ38334600
Graphene and other nanomaterial-based electrochemical aptasensorsQ30420976
Oligonucleotide aptamers that recognize small moleculesQ30710618
Short bioactive Spiegelmers to migraine-associated calcitonin gene-related peptide rapidly identified by a novel approach: tailored-SELEXQ30823550
In vivo selection of tumor-targeting RNA motifsQ30927258
Exploring the sequence space of a DNA aptamer using microarraysQ33304953
Minimal primer and primer-free SELEX protocols for selection of aptamers from random DNA librariesQ33325242
A synthetic molecular system capable of mirror-image genetic replication and transcriptionQ33363386
The shorter the better: reducing fixed primer regions of oligonucleotide libraries for aptamer selectionQ33433379
Aptamer-based 'point-of-care testing'.Q33447229
Generation of highly specific aptamers via micromagnetic selectionQ33456077
Monitoring genomic sequences during SELEX using high-throughput sequencing: neutral SELEX.Q33531798
A new measure for in vivo thrombin activity in comparison with in vitro thrombin generation potential in patients with hyper- and hypocoagulabilityQ33599937
Quantitative selection of DNA aptamers through microfluidic selection and high-throughput sequencingQ33655937
Bioimaging of nucleolin aptamer-containing 5-(N-benzylcarboxyamide)-2'-deoxyuridine more capable of specific binding to targets in cancer cellsQ33693099
Laboratory Measurement of Thrombin Activity--What Every Clinician Scientist Needs to KnowQ33801932
Probing high affinity sequences of DNA aptamer against VEGF165Q34168165
In vitro selection with artificial expanded genetic information systemsQ34394957
Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2'-modified nucleotidesQ34397679
X-aptamers: a bead-based selection method for random incorporation of druglike moieties onto next-generation aptamers for enhanced bindingQ34443595
Methods for measuring aptamer-protein equilibria: a reviewQ34518680
In vivo SELEX for Identification of Brain-penetrating Aptamers.Q34539526
Discrimination of recombinant from natural human growth hormone using DNA aptamers.Q34688648
Capillary electrophoresis-systematic evolution of ligands by exponential enrichment selection of base- and sugar-modified DNA aptamers: target binding dominated by 2'-O,4'-C-methylene-bridged/locked nucleic acid primerQ34714314
Identification of a novel prostate cancer susceptibility variant in the KLK3 gene transcriptQ34975918
A controllable aptamer-based self-assembled DNA dendrimer for high affinity targeting, bioimaging and drug deliveryQ35605916
Isolation of Foreign Material-Free Endothelial Progenitor Cells Using CD31 Aptamer and Therapeutic Application for Ischemic InjuryQ35684128
The Effects of SELEX Conditions on the Resultant Aptamer Pools in the Selection of Aptamers Binding to Bacterial Cells.Q35832906
DNA Aptamer Generation by Genetic Alphabet Expansion SELEX (ExSELEX) Using an Unnatural Base Pair SystemQ35836538
An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenes.Q35871867
The isolation of an RNA aptamer targeting to p53 protein with single amino acid mutation.Q35961417
Synthetic peptides derived from the Listeria monocytogenes p60 protein as antigens for the generation of polyclonal antibodies specific for secreted cell-free L. monocytogenes p60 proteinsQ36058071
Balancing between affinity and speed in target DNA search by zinc-finger proteins via modulation of dynamic conformational ensembleQ36079071
In vitro evolution of chemically-modified nucleic acid aptamers: Pros and cons, and comprehensive selection strategiesQ36156320
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P304page(s)41
P577publication date2018-05-14
P1433published inFrontiers in molecular biosciencesQ27726420
P1476titleSimple Methods and Rational Design for Enhancing Aptamer Sensitivity and Specificity.
P478volume5

Reverse relations

cites work (P2860)
Q92427810Differential binding cell-SELEX method to identify cell-specific aptamers using high-throughput sequencing
Q58717959G-Quadruplex-Forming DNA Aptamers Inhibit the DNA-Binding Function of HupB and Mycobacteriumtuberculosis Entry into Host Cells
Q99544596Paper-based fluorogenic RNA aptamer sensors for label-free detection of small molecules
Q60045589Rational truncation of aptamer for cross-species application to detect krait envenomation
Q64119627Structural switching electrochemical DNA aptasensor for the rapid diagnosis of tuberculous meningitis
Q92811652The Role of CYP450 Drug Metabolism in Precision Cardio-Oncology

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