Generation of β-globin by sequence-specific proteolysis of a hybrid protein produced in Escherichia coli

scientific article published on July 28, 1984

Generation of β-globin by sequence-specific proteolysis of a hybrid protein produced in Escherichia coli is …
instance of (P31):
scholarly articleQ13442814

External links are
P6179Dimensions Publication ID1035092927
P356DOI10.1038/309810A0
P953full work available at URLhttp://www.nature.com/articles/309810a0
http://www.nature.com/articles/309810a0.pdf
P698PubMed publication ID6330564

P2093author name stringK. Nagai
H. C. Thøgersen
P2860cites workDNA sequencing with chain-terminating inhibitorsQ22066207
Determinant of cistron specificity in bacterial ribosomesQ22122424
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applicationsQ24561689
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Q25938983
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersQ28131744
Amino acid sequence of human prothrombin fragments 1 and 2Q28646320
Construction of plasmids for expression of Rous sarcoma virus transforming protein, p60src, in Escherichia coliQ30450033
Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatinQ34051625
Insertion of synthetic copies of human globin genes into bacterial plasmidsQ36167079
Rapid synthesis of oligodeoxyribonucleotides. VII. Solid phase synthesis of oligodeoxyribonucleotides by a continuous flow phosphotriester method on a kieselguhr-polyamide supportQ40496800
Molecular cloning of the DNA ligase gene from bacteriophage T4. II. Amplification and preparation of the gene productQ41147281
The relationship between function and DNA sequence in an intercistronic regulatory region in phage λQ44424132
Improved plasmid vectors with a thermoinducible expression and temperature-regulated runaway replicationQ44773159
Plasmid vectors for high-efficiency expression controlled by the promoter of coliphage lambdaQ45139427
Bovine factor X1 (Stuart factor). Mechanism of activation by a protein from Russell's viper venomQ48025099
The nucleotide sequence of the human β-globin geneQ48412400
Bovine factors X1and X 2 (Stuart factor). Isolation and characterizationQ53968571
Expression of cloned β-endorphin gene sequences by Escherichia coliQ59090911
P433issue5971
P407language of work or nameEnglishQ1860
P921main subjectEscherichia coliQ25419
proteolysisQ33123
P304page(s)810-812
P577publication date1984-06-01
1984-07-28
P1433published inNatureQ180445
P1476titleGeneration of beta-globin by sequence-specific proteolysis of a hybrid protein produced in Escherichia coli
Generation of β-globin by sequence-specific proteolysis of a hybrid protein produced in Escherichia coli
P478volume309

Reverse relations

cites work (P2860)
Q6780575217 Catalytic antibodies
Q45786803A Sensitive Serodiagnosis of Hepatitis C Virus (HCV) Infection with Two Non‐Fused Peptides: Comparison of Antibody Responses Detected with a Newly Developed Assay and a Commercial Second‐Generation Test
Q68203612A fusion plasmid for the synthesis of lipopeptide-antigen chimeras in Escherichia coli
Q35090655A gene encoding a DnaK/hsp70 homolog in Escherichia coli
Q74025947A method for determining the in vivo topology of yeast polytopic membrane proteins demonstrates that Gap1p fully integrates into the membrane independently of Shr3p
Q40525942A novel protein (suef) programmed by the leftwards strand of a chimeric cDNA stops the expression of several proteins in E. coli
Q31153511A phosphorylation tag for uranyl mediated protein purification and photo assisted tag removal
Q48172851A single step purification for recombinant proteins Characterization of a microtubule associated protein (MAP 2) fragment which associates with the type II cAMP‐dependent protein kinase
Q54549989A unique approach for high level expression and production of a recombinant cobra neurotoxin in Escherichia coli.
Q40218662Addition of missing loops and domains to protein models by x-ray solution scattering
Q45164552An improved system for expressing pancreatic ribonuclease in Escherichia coli
Q37608972Analysis of Azotobacter vinelandii strains containing defined deletions in the nifD and nifK genes
Q31033116Applications of novel affinity cassette methods: use of peptide fusion handles for the purification of recombinant proteins
Q54570851Assembly of human hemoglobin. Studies with Escherichia coli-expressed alpha-globin.
Q38722477Bacterial expression of eukaryotic contractile proteins
Q34170060Beta spectrin kissimmee: a spectrin variant associated with autosomal dominant hereditary spherocytosis and defective binding to protein 4.1.
Q41536682Binding of the IS903 transposase to its inverted repeat in vitro
Q41769394Carbohydrate-recognition domains as tools for rapid purification of recombinant eukaryotic proteins
Q54697967Chimaeric myosin regulatory light chains: Sub-domain switching experiments to analyse the function of the N-terminal EF hand
Q54645017Chimeric hemoglobin subunits: functional properties of a recombinant beta/alpha hemoglobin.
Q36563985Cleavage of RNA hairpins mediated by a developmentally regulated CCCH zinc finger protein
Q53224878Cleavage oxygenases for the biosynthesis of trisporoids and other apocarotenoids in Phycomyces.
Q33257246Cloning and characterization of major antigenic determinants of human cytomegalovirus Ad169 seen by the human immune system
Q36489316Cloning, expression in Escherichia coli, and reconstitution of human myoglobin
Q30983390Cloning, sequencing, and overexpression of a [2Fe-2S] ferredoxin gene from Escherichia coli
Q37301645Coexpression of human alpha- and circularly permuted beta-globins yields a hemoglobin with normal R state but modified T state properties
Q54635384Collagen-binding recombinant fibronectin fragments containing type II domains.
Q44253367Conformational changes in hemoglobin S (betaE6V) imposed by mutation of the beta Glu7-beta Lys132 salt bridge and detected by UV resonance Raman spectroscopy.
Q45389702Covalent immobilization of tobacco-etch-virus NIa protease: a useful tool for cleavage of the histidine tag of recombinant proteins
Q22008058Crystal structure of deoxy-human hemoglobin beta6 Glu --> Trp. Implications for the structure and formation of the sickle cell fiber
Q74593454Cyanelle ribonuclease P: isolation and structure-function studies of an organellar ribonucleoprotein enzyme
Q39719865DNA binding and DNA bending by the MelR transcription activator protein from Escherichia coli
Q40405712Defining a minimal estrogen receptor DNA binding domain
Q46492201Deletion analysis of a multifunctional yeast tRNA ligase polypeptide. Identification of essential and dispensable functional domains
Q38352353Determination of the transmembrane topology of yeast Sec61p, an essential component of the endoplasmic reticulum translocation complex
Q27008891Development of recombinant hemoglobin-based oxygen carriers
Q34322852Developmental and hormonal regulation of the Xenopus liver-type arginase gene
Q33757233Different strategies for preparation of non-tagged rV270 protein and its efficacy against Yersinia pestis challenge
Q30404403Distal residues in the oxygen binding site of haemoglobin studied by protein engineering
Q33893034Downregulation of cardiac guanosine 5'-triphosphate-binding proteins in right atrium and left ventricle in pacing-induced congestive heart failure
Q35565633EcoK selection vectors for shotgun cloning into M13 and deletion mutagenesis.
Q70521477Effects of beta 6 amino acid hydrophobicity on stability and solubility of hemoglobin tetramers
Q36135940Efficient secretion and purification of human insulin-like growth factor I with a gene fusion vector in Staphylococci.
Q37710968Enhanced polymerization of recombinant human deoxyhemoglobin beta 6 Glu----Ile
Q27621801Enhanced visibility of hydrogen atoms by neutron crystallography on fully deuterated myoglobin
Q37152862Enzyme-linked immunosorbent assay for Escherichia coli heat-stable enterotoxin type II.
Q34011746Erv14p directs a transmembrane secretory protein into COPII-coated transport vesicles
Q33916286Expression and purification of recombinant hemoglobin in Escherichia coli
Q38310240Expression and purification of recombinant proteins by fusion to maltose-binding protein
Q70146292Expression in Escherichia coli of the genes coding for reaction center subunits from Rhodobacter sphaeroides: wild-type proteins and fusion proteins containing one or four truncated domains from Staphylococcus aureus protein A at the carboxy-terminu
Q33834209Expression of human ferredoxin and assembly of the [2Fe-2S] center in Escherichia coli
Q41584348Expression of human plasma gelsolin in Escherichia coli and dissection of actin binding sites by segmental deletion mutagenesis
Q40566586Expression of porcine pancreatic phospholipase A2. Generation of active enzyme by sequence-specific cleavage of a hybrid protein from Escherichia coli
Q67472150Expression of recombinant human hemoglobin
Q54578683Expression of soluble human beta-globin chains in bacteria and assembly in vitro with alpha-globin chains.
Q36063883Expression of wild-type and mutant bovine pancreatic ribonuclease A in Escherichia coli
Q38312015Expression, purification and characterization of the recombinant kringle 2 and kringle 3 domains of human plasminogen and analysis of their binding affinity for omega-aminocarboxylic acids
Q52441849Extracellular production system of heterologous peptide driven by a secretory protease inhibitor of Streptomyces
Q54714779Factor Xa cleavage of fusion proteins. Elimination of non-specific cleavage by reversible acylation.
Q71144001Functional studies and polymerization of recombinant hemoglobin Glu-alpha2beta26(A3) --> Val/Glu-7(A4) --> Ala
Q43586191Gastrodianin-like mannose-binding proteins: a novel class of plant proteins with antifungal properties
Q40425251Gene fusion expression systems in Escherichia coli
Q37401724Gene synthesis, expression, and mutagenesis of the blue copper proteins azurin and plastocyanin
Q71497683Generation of the cytosolic domain of microsomal P450 52A3 after high-level expression in Saccharomyces cerevisiae
Q30334357Genetic approaches to protein structure and function: point mutations as modifiers of protein function
Q44311691Haemoglobin alpha 2 beta 2 23Val----Ile produced in Escherichia coli facilitates Hb S polymerization
Q38159786Heterologous gene expression in Saccharomyces cerevisiae
Q34377722High-level expression of sperm whale myoglobin in Escherichia coli
Q42071342High-level production of human alpha- and beta-globins in insect cells
Q33323288Human Complement Factor H: Molecular Cloning and cDNA Expression Reveals Variability in the Factor H-Related mRNA Species of 1.4 kb
Q40227114Human androgen receptor expressed in HeLa cells activates transcription in vitro
Q53784490Hybrid myosin light chains containing a calcium-specific site from troponin C.
Q35963082Hyperexpression and purification of Escherichia coli adenylate cyclase using a vector designed for expression of lethal gene products
Q54599255Hyperproduction of a recombinant fusion protein of Staphylococcus aureus V8 protease in Escherichia coli and its processing by OmpT protease to release an active V8 protease derivative.
Q40230497Identification of a novel HIV-1 TAR RNA bulge binding protein
Q28305592Identification of a novel protein kinase A anchoring protein that binds both type I and type II regulatory subunits
Q35858034Improvement of retroviral retargeting by using amino acid spacers between an additional binding domain and the N terminus of Moloney murine leukemia virus SU.
Q50545034Indefinite noncooperative self-association of chicken deoxy hemoglobin D.
Q73413472Influence of the A helix structure on the polymerization of hemoglobin S
Q36000940Interactions of purified transcription factors: binding of yeast MAT alpha 1 and PRTF to cell type-specific, upstream activating sequences
Q35592631Isotope-detected 1H NMR studies of proteins: a general strategy for editing interproton nuclear Overhauser effects by heteronuclear decoupling, with application to phage lambda repressor
Q38297496Limited and defined truncation at the C terminus enhances receptor binding and degranulation activity of the neutrophil-activating peptide 2 (NAP-2). Comparison of native and recombinant NAP-2 variants
Q53732396Loss of calcium sensitivity of plasma gelsolin is associated with the presence of calcium ions during preparation.
Q39428755Low-scale expression and purification of an active putative iduronate 2-sulfate sulfatase-Like enzyme from Escherichia coli K12.
Q32051578Major antigenic proteins of the agent of human granulocytic ehrlichiosis are encoded by members of a multigene family.
Q40806982McrB: a prokaryotic protein specifically recognizing DNA containing modified cytosine residues.
Q40649868Modeling of active transport systems
Q73797166Modification of alpha-chain or beta-chain heme pocket polarity by Val(E11) --> thr substitution has different effects on the steric, dynamic, and functional properties of human recombinant hemoglobin. Deoxy derivatives
Q28580909Molecular cloning and nucleotide sequence of cDNA encoding rat kidney long-chain L-2-hydroxy acid oxidase. Expression of the catalytically active recombinant protein as a chimaera
Q36453511Molecular cloning and sequencing of the chicken smooth muscle myosin regulatory light chain
Q67472155Molecular engineering of hemoglobin for transfusion
Q28572877Mutations in the second cytoplasmic loop of the rat parathyroid hormone (PTH)/PTH-related protein receptor result in selective loss of PTH-stimulated phospholipase C activity
Q36235831NAC covers ribosome-associated nascent chains thereby forming a protective environment for regions of nascent chains just emerging from the peptidyl transferase center
Q47858897Nuclear magnetic resonance spectroscopy in the study of hemoglobin cooperativity
Q37536158Oxygen binding properties of human mutant hemoglobins synthesized in Escherichia coli
Q54634582Phage display of enzymes and in vitro selection for catalytic activity.
Q42980633Phosphorylation of recombinant human phenylalanine hydroxylase: effect on catalytic activity, substrate activation and protection against non-specific cleavage of the fusion protein by restriction protease
Q38296289Poliovirus protein 2C contains two regions involved in RNA binding activity
Q44011073Polymerization and solubility of recombinant hemoglobins alpha 2 beta 2 (6Val) (Hb S) and alpha 2 beta 2(6Leu) (Hb Leu).
Q47854748Production of recombinant bovine enterokinase catalytic subunit in Escherichia coli using the novel secretory fusion partner DsbA.
Q54423628Production of recombinant proteins in Escherichia coli.
Q36510636Production of unmodified human adult hemoglobin in Escherichia coli
Q42642293Proliferative T-cell response to glycoprotein B of the human herpes viruses: the influence of MHC and sequence of infection on the pattern of cross-reactivity
Q35533714Proton nuclear magnetic resonance studies on hemoglobin: cooperative interactions and partially ligated intermediates
Q32071807Purification and properties of BiP.
Q40508786Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies
Q59068600Recombinant antibodies possessing novel effector functions
Q44454698Recombinant expression of Mus musculus myoglobin
Q39929694Recombinant expression of the pufQ gene of Rhodobacter capsulatus
Q67870996Recombinant human hemoglobin: expression and refolding of beta-globin from Escherichia coli
Q36081432Recombinant human sickle hemoglobin expressed in yeast
Q30414571Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications
Q35553783Reduced beta-adrenergic receptor activation decreases G-protein expression and beta-adrenergic receptor kinase activity in porcine heart
Q33909629Regional myocardial downregulation of the inhibitory guanosine triphosphate-binding protein (Gi alpha 2) and beta-adrenergic receptors in a porcine model of chronic episodic myocardial ischemia
Q38560672Response of digestive cysteine proteinases from the Colorado potato beetle (Leptinotarsa decemlineata) and the black vine weevil (Otiorynchus sulcatus) to a recombinant form of human stefin A.
Q41089232Secondary structure and Ca2+-induced conformational change of calexcitin, a learning-associated protein
Q38566357Selective inhibition of Colorado potato beetle cathepsin H by oryzacystatins I and II.
Q67528886Sequence-specific cleavage of protein fusions using a recombinant Neisseria type 2 IgA protease
Q24308996Simian virus 40 large T antigen affects the Saccharomyces cerevisiae cell cycle and interacts with p34CDC28
Q54778038Site-directed mutagenesis of the regulatory light-chain Ca2+/Mg2+ binding site and its role in hybrid myosins.
Q31020943Site-directed mutation makes rabbit calcyclin dimer
Q36277365Steric and hydrophobic determinants of the solubilities of recombinant sickle cell hemoglobins
Q36671977Strategies for achieving high-level expression of genes in Escherichia coli
Q37785042Strategies to Optimize Protein Expression in E. coli
Q74392857Structural and functional effects of pseudo-module substitution in hemoglobin subunits. New structural and functional units in globin structure
Q73889930Structural and functional roles of modules in hemoglobin. Substitution of module M4 in hemoglobin subunits
Q35027925Structural biology and function of solute transporters: implications for identifying and designing substrates
Q38722458Structure-function analysis of thin filament proteins expressed in Escherichia coli
Q73704018Substitution of the heme binding module in hemoglobin alpha- and beta-subunits. Implication for different regulation mechanisms of the heme proximal structure between hemoglobin and myoglobin
Q97597136Synthesis of Recombinant Human Hemoglobin With NH2 -Terminal Acetylation in Escherichia coli
Q37411267Synthesis, bacterial expression, and mutagenesis of the gene coding for mammalian cytochrome b5.
Q36280864Tetramer-dimer equilibrium of oxyhemoglobin mutants determined from auto-oxidation rates
Q54600214Tetrameric hemoglobin expressed in Escherichia coli. Evidence of heterogeneous subunit assembly.
Q44190110The A-kinase anchoring domain of type IIalpha cAMP-dependent protein kinase is highly helical
Q28203819The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins
Q67426632The leucine zipper domain controls the orientation of AP-1 in the NFAT.AP-1.DNA complex
Q27931338The mitochondrial message-specific mRNA protectors Cbp1 and Pet309 are associated in a high-molecular weight complex.
Q54738832The role of the distal histidine in myoglobin and haemoglobin.
Q74303832The role of the membrane-spanning domain of type I signal peptidases in substrate cleavage site selection
Q38315694Transcriptional control of the invasion regulatory gene virB of Shigella flexneri: activation by virF and repression by H-NS
Q24628944Translation of a synthetic two-cistron mRNA in Escherichia coli
Q41350511Truncation of the carboxyl-terminal region of the rat parathyroid hormone (PTH)/PTH-related peptide receptor enhances PTH stimulation of adenylyl cyclase but not phospholipase C.
Q48043739TrwD, a protein encoded by the IncW plasmid R388, displays an ATP hydrolase activity essential for bacterial conjugation
Q42844945Two mutations in recombinant Hb beta F41(C7)Y, K82 (EF6)D show additive effects in decreasing oxygen affinity
Q51198062Upregulation of an Artificial Zymogen by Proteolysis.
Q35103241Urinary excretion of fatty acid-binding protein reflects stress overload on the proximal tubules
Q40468019Utilization of Escherichia coli outer-membrane endoprotease OmpT variants as processing enzymes for production of peptides from designer fusion proteins.

Search more.