| review article | Q7318358 |
| scholarly article | Q13442814 |
| P50 | author | Bon-Kyoung Koo | Q61208762 |
| Hugo J Snippert | Q82270386 | ||
| Yannik Bollen | Q89338161 | ||
| P2093 | author name string | Jasmin Post | |
| P2860 | cites work | Modeling Development and Disease with Organoids | Q24657457 |
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| The molecular targets of resveratrol | Q26866214 | ||
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| Functional repair of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients | Q28303521 | ||
| Differential roles for Sox15 and Sox2 in transcriptional control in mouse embryonic stem cells | Q28505222 | ||
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| Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins. | Q30356050 | ||
| Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo | Q30614255 | ||
| CRISPR-Cpf1 correction of muscular dystrophy mutations in human cardiomyocytes and mice. | Q33558467 | ||
| Biallelic insertion of a transcriptional terminator via the CRISPR/Cas9 system efficiently silences expression of protein-coding and non-coding RNA genes | Q33567619 | ||
| Ten years of gene targeting: targeted mouse mutants, from vector design to phenotype analysis | Q33650414 | ||
| Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins | Q33657682 | ||
| High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs | Q33698508 | ||
| In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni | Q33807299 | ||
| CRISPR/Cas9 - Mediated Precise Targeted Integration In Vivo Using a Double Cut Donor with Short Homology Arms | Q33817794 | ||
| Targeted gene knock-in by homology-directed genome editing using Cas9 ribonucleoprotein and AAV donor delivery | Q33878793 | ||
| A novel system for the production of fully deleted adenovirus vectors that does not require helper adenovirus | Q33952908 | ||
| Mechanisms of DNA double-strand break repair and their potential to induce chromosomal aberrations | Q33962417 | ||
| Regulation of homologous recombination in eukaryotes | Q33966065 | ||
| Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis | Q34037839 | ||
| Genome editing with Cas9 in adult mice corrects a disease mutation and phenotype. | Q34040385 | ||
| In vivo genome editing using Staphylococcus aureus Cas9 | Q34043628 | ||
| Cloning-free CRISPR | Q34045288 | ||
| Rationally engineered Cas9 nucleases with improved specificity | Q34045530 | ||
| Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage | Q34046684 | ||
| Conservative homologous recombination preferentially repairs DNA double-strand breaks in the S phase of the cell cycle in human cells | Q40536064 | ||
| The length of homology required for gene targeting in embryonic stem cells | Q40643090 | ||
| Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. | Q41562658 | ||
| In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting | Q41689807 | ||
| Simple and rapid in vivo generation of chromosomal rearrangements using CRISPR/Cas9 technology | Q41747565 | ||
| A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells | Q41992926 | ||
| Synthetically modified guide RNA and donor DNA are a versatile platform for CRISPR-Cas9 engineering | Q42289899 | ||
| Generating rats with conditional alleles using CRISPR/Cas9. | Q42762266 | ||
| Rescue of high-specificity Cas9 variants using sgRNAs with matched 5' nucleotides | Q46148608 | ||
| CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing | Q46244913 | ||
| Temperature effect on CRISPR-Cas9 mediated genome editing. | Q46382576 | ||
| Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing | Q47106725 | ||
| Mechanisms of precise genome editing using oligonucleotide donors | Q47110165 | ||
| CRISPR/Cas9 delivery with one single adenoviral vector devoid of all viral genes | Q47117286 | ||
| Scarless Genome Editing of Human Pluripotent Stem Cells via Transient Puromycin Selection | Q47196011 | ||
| Precise and efficient nucleotide substitution near genomic nick via noncanonical homology-directed repair | Q47223201 | ||
| Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors. | Q47229150 | ||
| Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaks | Q47305432 | ||
| Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage | Q47328087 | ||
| Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing. | Q47355595 | ||
| In vivo genome editing in animals using AAV-CRISPR system: applications to translational research of human disease | Q47553772 | ||
| A 'new lease of life': FnCpf1 possesses DNA cleavage activity for genome editing in human cells. | Q47601711 | ||
| The Position of the GFP Tag on Actin Affects the Filament Formation in Mammalian Cells | Q47737706 | ||
| Partial DNA-guided Cas9 enables genome editing with reduced off-target activity. | Q48152553 | ||
| Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. | Q48230762 | ||
| Seamless genome editing in human pluripotent stem cells using custom endonuclease-based gene targeting and the piggyBac transposon | Q48925635 | ||
| 'Cold shock' increases the frequency of homology directed repair gene editing in induced pluripotent stem cells | Q49163431 | ||
| Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells | Q50024744 | ||
| Improving CRISPR-Cas specificity with chemical modifications in single-guide RNAs | Q50027400 | ||
| Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA. | Q51100477 | ||
| Embryonic development is disrupted by modest increases in vascular endothelial growth factor gene expression. | Q52165853 | ||
| Dissecting the role of N-myc in development using a single targeting vector to generate a series of alleles. | Q52186654 | ||
| Targeted deletion of 5'HS2 of the murine beta-globin LCR reveals that it is not essential for proper regulation of the beta-globin locus. | Q52205977 | ||
| Small molecules enhance CRISPR/Cas9-mediated homology-directed genome editing in primary cells | Q38615359 | ||
| Homology-mediated end joining-based targeted integration using CRISPR/Cas9. | Q38702939 | ||
| In vivo genome editing and organoid transplantation models of colorectal cancer and metastasis | Q38705367 | ||
| Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA. | Q38801430 | ||
| MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems. | Q38811005 | ||
| Efficient genetic engineering of human intestinal organoids using electroporation | Q38838399 | ||
| Visualization and targeting of LGR5+ human colon cancer stem cells. | Q38869171 | ||
| Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. | Q38895804 | ||
| Obligate ligation-gated recombination (ObLiGaRe): custom-designed nuclease-mediated targeted integration through nonhomologous end joining | Q39244770 | ||
| Efficient dual sgRNA-directed large gene deletion in rabbit with CRISPR/Cas9 system | Q40053525 | ||
| Quantification of Protein Levels in Single Living Cells | Q40189898 | ||
| Cell-Penetrating Peptide-Mediated Delivery of Cas9 Protein and Guide RNA for Genome Editing. | Q40480698 | ||
| Genome engineering using CRISPR-Cas9 system. | Q52423246 | ||
| Modeling colorectal cancer using CRISPR-Cas9-mediated engineering of human intestinal organoids. | Q52423411 | ||
| Sequential cancer mutations in cultured human intestinal stem cells. | Q52423597 | ||
| Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection. | Q52423691 | ||
| CRISPR/Cas9-Mediated Genome Editing of Mouse Small Intestinal Organoids. | Q52425380 | ||
| Ligase IV inhibitor SCR7 enhances gene editing directed by CRISPR-Cas9 and ssODN in human cancer cells. | Q52430423 | ||
| Microhomology-assisted scarless genome editing in human iPSCs. | Q52430597 | ||
| Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. | Q52430644 | ||
| Easy quantification of template-directed CRISPR/Cas9 editing. | Q52430807 | ||
| Efficient In Vivo Liver-Directed Gene Editing Using CRISPR/Cas9 | Q52431143 | ||
| Small molecules promote CRISPR-Cpf1-mediated genome editing in human pluripotent stem cells. | Q52431255 | ||
| Design and assessment of engineered CRISPR-Cpf1 and its use for genome editing. | Q52431346 | ||
| Engineering CRISPR/Cpf1 with tRNA promotes genome editing capability in mammalian systems. | Q52431448 | ||
| An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein. | Q52431454 | ||
| Review of CRISPR/Cas9 sgRNA Design Tools. | Q52431487 | ||
| Chemically Modified Cpf1-CRISPR RNAs Mediate Efficient Genome Editing in Mammalian Cells. | Q52431512 | ||
| Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity. | Q52431554 | ||
| Short DNA sequences inserted for gene targeting can accidentally interfere with off-target gene expression. | Q52598355 | ||
| In vitro synthesis of gene-length single-stranded DNA. | Q55080492 | ||
| CLICK: one-step generation of conditional knockout mice. | Q55295965 | ||
| Factors affecting the efficiency of introducing precise genetic changes in ES cells by homologous recombination: tag-and-exchange versus the Cre-loxp system | Q60287328 | ||
| Efficient intracellular delivery of native proteins | Q87181880 | ||
| Chimeric Guides Probe and Enhance Cas9 Biochemical Activity | Q88626084 | ||
| CRISPR-mediated direct mutation of cancer genes in the mouse liver | Q34353586 | ||
| Chromosomal site-specific double-strand breaks are efficiently targeted for repair by oligonucleotides in yeast | Q34389334 | ||
| Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. | Q34436638 | ||
| GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases | Q34454104 | ||
| Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining | Q34468164 | ||
| Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells | Q34482784 | ||
| High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. | Q34507554 | ||
| Structure and Engineering of Francisella novicida Cas9. | Q34514052 | ||
| An enhanced computational platform for investigating the roles of regulatory RNA and for identifying functional RNA motifs | Q34569900 | ||
| Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9 | Q34677703 | ||
| Easy quantitative assessment of genome editing by sequence trace decomposition | Q34712139 | ||
| Rapid modelling of cooperating genetic events in cancer through somatic genome editing | Q34938584 | ||
| Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells | Q35088938 | ||
| Efficient mutagenesis of the rhodopsin gene in rod photoreceptor neurons in mice | Q35153494 | ||
| Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery | Q35247829 | ||
| Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. | Q35896845 | ||
| Selectable one-step PCR-mediated integration of a degron for rapid depletion of endogenous human proteins | Q35913831 | ||
| CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer single-stranded DNA | Q35917382 | ||
| Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA | Q35999041 | ||
| Targeted gene knockout by direct delivery of zinc-finger nuclease proteins | Q36179350 | ||
| In vivo high-throughput profiling of CRISPR-Cpf1 activity | Q36227944 | ||
| Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition | Q36398694 | ||
| Baculoviral delivery of CRISPR/Cas9 facilitates efficient genome editing in human cells | Q36412556 | ||
| Delivery of Cas9 Protein into Mouse Zygotes through a Series of Electroporation Dramatically Increases the Efficiency of Model Creation | Q36968247 | ||
| Co-incident insertion enables high efficiency genome engineering in mouse embryonic stem cells. | Q37264419 | ||
| CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity | Q37271186 | ||
| Biasing genome-editing events toward precise length deletions with an RNA-guided TevCas9 dual nuclease. | Q37549944 | ||
| Method for Dual Viral Vector Mediated CRISPR-Cas9 Gene Disruption in Primary Human Endothelial Cells | Q37642712 | ||
| Efficient precise knockin with a double cut HDR donor after CRISPR/Cas9-mediated double-stranded DNA cleavage | Q37655265 | ||
| In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration | Q37673657 | ||
| Intracellular DNA recognition | Q37680669 | ||
| Enrichment of G2/M cell cycle phase in human pluripotent stem cells enhances HDR-mediated gene repair with customizable endonucleases | Q38542079 | ||
| Inhibition of nonhomologous end joining to increase the specificity of CRISPR/Cas9 genome editing | Q38569880 | ||
| P275 | copyright license | Creative Commons Attribution-NonCommercial 4.0 International | Q34179348 |
| P433 | issue | 13 | |
| P407 | language of work or name | English | Q1860 |
| P921 | main subject | regulation of gene expression | Q411391 |
| CRISPR | Q412563 | ||
| gene editing | Q65363531 | ||
| biomedical investigative technique | Q66648976 | ||
| CRISPR-Cas systems | Q71149179 | ||
| P1104 | number of pages | 20 | |
| P304 | page(s) | 6435-6454 | |
| P577 | publication date | 2018-07-01 | |
| 2018-07-27 | |||
| P1433 | published in | Nucleic Acids Research | Q135122 |
| P1476 | title | How to create state-of-the-art genetic model systems: strategies for optimal CRISPR-mediated genome editing | |
| P478 | volume | 46 |
| Q90071246 | A Broad Application of CRISPR Cas9 in Infectious Diseases of Central Nervous System |
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| Q89727321 | Colorectal Cancer Modeling with Organoids: Discriminating between Oncogenic RAS and BRAF Variants |
| Q98463447 | Engineering designer beta cells with a CRISPR-Cas9 conjugation platform |
| Q64080122 | Gene-edited babies: What went wrong and what could go wrong |
| Q99721471 | Precise Correction of Heterozygous SHOX2 Mutations in hiPSCs Derived from Patients with Atrial Fibrillation via Genome Editing and Sib Selection |
| Q91225440 | Rapid Tagging of Human Proteins with Fluorescent Reporters by Genome Engineering using Double-Stranded DNA Donors |
| Q91858731 | Understanding off-target effects through hybridization kinetics and thermodynamics |
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