Increased Sensitivity in PCR Detection of tdh-Positive Vibrio parahaemolyticus in Seafood with Purified Template DNA

scientific article published on 01 September 2003

Increased Sensitivity in PCR Detection of tdh-Positive Vibrio parahaemolyticus in Seafood with Purified Template DNA is …
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scholarly articleQ13442814

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P356DOI10.4315/0362-028X-66.9.1675
P953full work available at URLhttp://apt.allenpress.com/aptonline/?request=get-abstract&issn=0362-028X&volume=066&issue=09&page=1675
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P698PubMed publication ID14503724

P2093author name stringA. Kiuchi
S. Kumagai
T. Kawasumi
Y. Kasuga
T. Horisaka
Y. Hara-Kudo
P2860cites workImproved method for detection of Vibrio parahaemolyticus in seafoodQ33990954
Improved template preparation for PCR-based assays for detection of food-borne bacterial pathogensQ39487996
A PCR-based assay for the detection of Escherichia coli Shiga-like toxin genes in ground beefQ39799605
In Vitro Hemolytic Characteristic of Vibrio parahaemolyticus : Its Close Correlation with Human PathogenicityQ40113000
The direct application of the polymerase chain reaction to DNA extracted from foodsQ41494946
Detection of total and hemolysin-producing Vibrio parahaemolyticus in shellfish using multiplex PCR amplification of tl, tdh and trh.Q43338008
Use of the polymerase chain reaction for direct detection of Listeria monocytogenes in soft cheeseQ44435743
Evaluation of different DNA extraction procedures for the detection of Salmonella from chicken products by polymerase chain reaction.Q50146847
Detection of Shigella spp. in food with a nested PCR method-sensitivity and performance compared with a conventional culture method.Q50517433
Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutionsQ67979852
Detection of the thermostable direct hemolysin gene (tdh) and the thermostable direct hemolysin-related hemolysin gene (trh) of Vibrio parahaemolyticus by polymerase chain reactionQ67984016
Rapid polymerase chain reaction method for detection of Kanagawa positive Vibrio parahaemolyticus in seafoodsQ71704054
Rapid and specific detection of the thermostable direct haemolysin gene in Vibrio parahaemolyticus by the polymerase chain reactionQ72288851
Comparison of Template Preparation Methods from Foods for Amplification of Escherichia coli O157 Shiga-Like Toxins Type I and II DNA by Multiplex Polymerase Chain ReactionQ92347488
P433issue9
P407language of work or nameEnglishQ1860
P921main subjectVibrio parahaemolyticusQ134280
microbiologyQ7193
food scienceQ1637030
P1104number of pages6
P304page(s)1675-1680
P577publication date2003-09-01
P1433published inJournal of Food ProtectionQ15761591
P1476titleIncreased sensitivity in PCR detection of tdh-positive Vibrio parahaemolyticus in seafood with purified template DNA
Increased Sensitivity in PCR Detection of tdh-Positive Vibrio parahaemolyticus in Seafood with Purified Template DNA
P478volume66