Comparison of LC and LC/MS methods for quantifying N-glycosylation in recombinant IgGs

scientific article published on 16 July 2008

Comparison of LC and LC/MS methods for quantifying N-glycosylation in recombinant IgGs is …
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scholarly articleQ13442814

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P6179Dimensions Publication ID1020303650
P356DOI10.1016/J.JASMS.2008.07.004
P698PubMed publication ID18707900

P2093author name stringElizabeth M Topp
Pavel V Bondarenko
Himanshu S Gadgil
Michael J Treuheit
Gary Pipes
Sandipan Sinha
P2860cites workStructural analysis of human IgG-Fc glycoforms reveals a correlation between glycosylation and structural integrityQ27640312
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The effect on IgG glycosylation of altering beta1, 4-galactosyltransferase-1 activity in B cellsQ40987105
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The products from papain and pepsin hydrolyses of guinea-pig immunoglobulins gamma 1G and gamma 2 G.Q42922054
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Kinetics of whole serum and prepurified IgG digestion by pepsin for F(ab')2 manufactureQ44533374
Improving mass accuracy of high performance liquid chromatography/electrospray ionization time-of-flight mass spectrometry of intact antibodiesQ46091062
Reversed-phase liquid chromatography-mass spectrometry of site-specific chemical modifications in intact immunoglobulin molecules and their fragmentsQ46838930
Reversed-phase liquid chromatography of immunoglobulin G molecules and their fragments with the diphenyl columnQ46916515
The covalent structure of an entire gamma G immunoglobulin molecule. 1969.Q48646207
Contrasting glycosylation profiles between Fab and Fc of a human IgG protein studied by electrospray ionization mass spectrometry.Q53532844
Identification of cysteinylation of a free cysteine in the Fab region of a recombinant monoclonal IgG1 antibody using Lys-C limited proteolysis coupled with LC/MS analysis.Q53612735
Validation of a peptide mapping method for a therapeutic monoclonal antibody: what could we possibly learn about a method we have run 100 times?Q73524714
The LC/MS analysis of glycation of IgG molecules in sucrose containing formulationsQ80591216
P433issue11
P304page(s)1643-1654
P577publication date2008-07-16
P1433published inJournal of the American Society for Mass SpectrometryQ3020250
P1476titleComparison of LC and LC/MS methods for quantifying N-glycosylation in recombinant IgGs
P478volume19