An optimized culture system for notochordal cell expansion with retention of phenotype

scientific article published on 26 July 2018

An optimized culture system for notochordal cell expansion with retention of phenotype is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1002/JSP2.1028
P932PMC publication ID6686815
P698PubMed publication ID31463448

P50authorStephen M RichardsonQ40727008
Matthew HumphreysQ61105976
Lizzy WardQ67385357
Judith A. HoylandQ67385365
P2860cites workSoft substrates promote homogeneous self-renewal of embryonic stem cells via downregulating cell-matrix tractionsQ21091073
Identification of nucleus pulposus precursor cells and notochordal remnants in the mouse: implications for disk degeneration and chordoma formationQ24649542
Regulation of hypoxia-inducible factor 1alpha is mediated by an O2-dependent degradation domain via the ubiquitin-proteasome pathwayQ24672316
Human disc cells in monolayer vs 3D culture: cell shape, division and matrix formationQ24799188
Tissue cells feel and respond to the stiffness of their substrateQ29547613
Geometric control of cell life and deathQ29615213
Cell locomotion and focal adhesions are regulated by substrate flexibilityQ29615522
Substrate rigidity regulates the formation and maintenance of tissuesQ30476924
Matrix metalloproteinase 28, a novel matrix metalloproteinase, is constitutively expressed in human intervertebral disc tissue and is present in matrix of more degenerated discsQ33517863
Notochordal cell conditioned medium stimulates mesenchymal stem cell differentiation toward a young nucleus pulposus phenotypeQ33999475
Extracellular matrix ligand and stiffness modulate immature nucleus pulposus cell-cell interactionsQ34075531
Regulation of HIF by the von Hippel-Lindau tumour suppressor: implications for cellular oxygen sensing.Q34497627
Activation of intervertebral disc cells by co-culture with notochordal cells, conditioned medium and hypoxiaQ34969332
The role of the physicochemical environment in determining disc cell behaviourQ35003373
Loss of notochordal cell phenotype in 3D-cell cultures: implications for disc physiology and disc repairQ35368899
Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells.Q35557848
Regenerative potential of TGFβ3 + Dex and notochordal cell conditioned media on degenerated human intervertebral disc cellsQ35691009
Notochordal cells protect nucleus pulposus cells from degradation and apoptosis: implications for the mechanisms of intervertebral disc degenerationQ35907855
The role of cytokines in cartilage matrix degeneration in osteoarthritis.Q35916137
Nutrition of the intervertebral discQ35963858
Hypoxia-inducible factor 1: regulation by hypoxic and non-hypoxic activatorsQ35993096
Human mesenchymal stem cells cultured on silk hydrogels with variable stiffness and growth factor differentiate into mature smooth muscle cell phenotypeQ36505550
Matrix synthesis and degradation in human intervertebral disc degeneration.Q36883824
Spatiotemporal analysis of putative notochordal cell markers reveals CD24 and keratins 8, 18, and 19 as notochord-specific markers during early human intervertebral disc developmentQ37252473
Development and regeneration potential of the mammalian intervertebral discQ38042320
Transcriptional regulation by hypoxia inducible factorsQ38149945
Influence of rabbit notochordal cells on symptomatic intervertebral disc degeneration: anti-angiogenic capacity on human endothelial cell proliferation under hypoxiaQ38401597
Notochordal cells influence gene expression of inflammatory mediators of annulus fibrosus cells in proinflammatory cytokines stimulationQ38482555
Swelling pressure of the lumbar intervertebral discs: influence of age, spinal level, composition, and degenerationQ39298783
Cells from different regions of the intervertebral disc: effect of culture system on matrix expression and cell phenotypeQ39602300
Notochordal intervertebral disc cells: sensitivity to nutrient deprivationQ39879588
The regenerative capacity of the notochordal cell: tissue constructs generated in vitro under hypoxic conditionsQ39967728
Modified expression of the ADAMTS enzymes and tissue inhibitor of metalloproteinases 3 during human intervertebral disc degenerationQ40006569
Molecular phenotypes of notochordal cells purified from immature nucleus pulposusQ40341786
How deeply cells feel: methods for thin gels.Q40363506
Tumor necrosis factor alpha and nucleus-pulposus-induced nerve root injuryQ40831742
Dose- and time-dependent effect of high glucose concentration on viability of notochordal cells and expression of matrix degrading and fibrotic enzymesQ41046146
The structure and degradation of aggrecan in human intervertebral discQ41854830
Matrix elasticity, cytoskeletal forces and physics of the nucleus: how deeply do cells 'feel' outside and in?Q41872279
Nucleus pulposus cell-matrix interactions with laminins.Q41957108
Identifying molecular phenotype of nucleus pulposus cells in human intervertebral disc with aging and degenerationQ42056281
Rat Notochordal Cells Undergo Premature Stress-Induced Senescence by High GlucoseQ42146019
p38 MAPK inhibition modulates rabbit nucleus pulposus cell response to IL-1.Q42525115
High glucose-induced oxidative stress promotes autophagy through mitochondrial damage in rat notochordal cellsQ42861656
Integrin-mediated interactions with extracellular matrix proteins for nucleus pulposus cells of the human intervertebral discQ42909500
The Notochord, Notochordal cell and CTGF/CCN-2: ongoing activity from development through maturationQ43173820
Intervertebral disc cells exhibit differences in gene expression in alginate and monolayer cultureQ43700372
Role of cell shape in growth controlQ44152787
Integrin expression in cells of the intervertebral discQ44937854
Distinction between the extracellular matrix of the nucleus pulposus and hyaline cartilage: a requisite for tissue engineering of intervertebral disc.Q45189368
Environmental regulation of notochordal gene expression in nucleus pulposus cells.Q45995067
Notochordal cells stimulate migration of cartilage end plate chondrocytes of the intervertebral disc in in vitro cell migration assaysQ46488671
The species-specific regenerative effects of notochordal cell-conditioned medium on chondrocyte-like cells derived from degenerated human intervertebral discs.Q46666807
Nucleus pulposus cells express HIF-1 alpha under normoxic culture conditions: a metabolic adaptation to the intervertebral disc microenvironmentQ46891204
Potential application of notochordal cells for intervertebral disc regeneration: an in vitro assessmentQ46958774
The Stimulatory Effect of Notochordal Cell-Conditioned Medium in a Nucleus Pulposus Explant CultureQ50243640
Relative increase of biglycan and decorin and altered chondroitin sulfate epitopes in the degenerating human intervertebral disc.Q50525024
Functional integrin subunits regulating cell-matrix interactions in the intervertebral disc.Q51062139
Nucleus pulposus notochord cells secrete connective tissue growth factor and up-regulate proteoglycan expression by intervertebral disc chondrocytes.Q51099346
Osmoregulatory function of large vacuoles found in notochordal cells of the intervertebral disc running title: an osmoregulatory vacuole.Q51789515
Positive feedback loop of interleukin-1beta upregulating production of inflammatory mediators in human intervertebral disc cells in vitro.Q53620494
Localization of degradative enzymes and their inhibitors in the degenerate human intervertebral discQ61699199
Ultrastructure of the human intervertebral disc: II. Cells of the nucleus pulposusQ70623627
Herniated lumbar intervertebral discs spontaneously produce matrix metalloproteinases, nitric oxide, interleukin-6, and prostaglandin E2Q71350636
Induction of matrix metalloproteinase-2 and -3 activity in ovine nucleus pulposus cells grown in three-dimensional agarose gel culture by interleukin-1beta: a potential pathway of disc degenerationQ73015644
Viscoelastic properties of intervertebral disc cells. Identification of two biomechanically distinct cell populationsQ73335571
Immunolocalization of major interstitial collagen types in human lumbar intervertebral discs of various agesQ74175553
Normoxic stabilization of HIF-1alpha drives glycolytic metabolism and regulates aggrecan gene expression in nucleus pulposus cells of the rat intervertebral diskQ80177979
Cytomorphology of notochordal and chondrocytic cells from the nucleus pulposus: a species comparisonQ81087892
Prolonged upright posture induces degenerative changes in intervertebral discs of rat cervical spineQ85198768
Increased osmolarity and cell clustering preserve canine notochordal cell phenotype in cultureQ86889057
P433issue3
P304page(s)e1028
P577publication date2018-07-26
P1433published inJOR SpineQ73541371
P1476titleAn optimized culture system for notochordal cell expansion with retention of phenotype
P478volume1

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