Producing defucosylated antibodies with enhanced in vitro antibody-dependent cellular cytotoxicity via FUT8 knockout CHO-S cells

scientific article published on 18 April 2017

Producing defucosylated antibodies with enhanced in vitro antibody-dependent cellular cytotoxicity via FUT8 knockout CHO-S cells is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1002/ELSC.201600255
P932PMC publication ID6999357
P698PubMed publication ID32624826

P50authorHuifang ZongQ97094408
Jianrong XuQ97094409
P2093author name stringHua Jiang
Kai Ding
Tao Sun
Jianwei Zhu
Lei Han
Cedric Cagliero
Baohong Zhang
Yueqing Xie
Xinyu Zhang
Jiaxian Wang
P2860cites workCrystal structure of mammalian alpha1,6-fucosyltransferase, FUT8Q24337064
Inhibitory Fc receptors modulate in vivo cytotoxicity against tumor targetsQ28140238
Improving the efficacy of antibody-based cancer therapiesQ28208361
Lack of fucose on human IgG1 N-linked oligosaccharide improves binding to human Fcgamma RIII and antibody-dependent cellular toxicityQ30835165
Characterization of genomic deletion efficiency mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 nuclease system in mammalian cells.Q33985245
Human Epidermal Growth Factor Receptor 2 (HER2) in Cancers: Overexpression and Therapeutic ImplicationsQ34223494
Establishment of FUT8 knockout Chinese hamster ovary cells: an ideal host cell line for producing completely defucosylated antibodies with enhanced antibody-dependent cellular cytotoxicityQ34346238
Variable contribution of monoclonal antibodies to ADCC in patients with chronic lymphocytic leukemiaQ34989602
Phase 1/2 study of mogamulizumab, a defucosylated anti-CCR4 antibody, in previously treated patients with cutaneous T-cell lymphomaQ35222112
Morphological and molecular characteristics of HER2 amplified urothelial bladder cancerQ35698114
Role of antibody-dependent cell-mediated cytotoxicity in the efficacy of therapeutic anti-cancer monoclonal antibodies.Q36366519
Protein A chromatography for antibody purificationQ36618496
Glycosylation as a strategy to improve antibody-based therapeuticsQ37402407
Cell engineering and cultivation of chinese hamster ovary (CHO) cells.Q37704492
Mammalian cell protein expression for biopharmaceutical productionQ37941925
Therapeutic antibodies: market considerations, disease targets and bioprocessingQ37974006
Targeting the human epidermal growth factor receptor 2 in esophageal cancerQ38097675
Expanding the genetic editing tool kit: ZFNs, TALENs, and CRISPR-Cas9.Q38255828
Enhanced natural killer cell binding and activation by low-fucose IgG1 antibody results in potent antibody-dependent cellular cytotoxicity induction at lower antigen densityQ38328896
CRISPR/Cas9-mediated genome engineering of CHO cell factories: Application and perspectivesQ38522356
Production process reproducibility and product quality consistency of transient gene expression in HEK293 cells with anti-PD1 antibody as the model proteinQ38730765
CRISPR-Cas targeted plasmid integration into mammalian cells via non-homologous end joiningQ38878854
Trastuzumab induces antibody-dependent cell-mediated cytotoxicity (ADCC) in HER-2-non-amplified breast cancer cell linesQ39447473
Highly efficient deletion of FUT8 in CHO cell lines using zinc-finger nucleases yields cells that produce completely nonfucosylated antibodies.Q39690283
Trastuzumab causes antibody-dependent cellular cytotoxicity-mediated growth inhibition of submacroscopic JIMT-1 breast cancer xenografts despite intrinsic drug resistanceQ40109415
Trastuzumab-mediated antibody-dependent cellular cytotoxicity against esophageal squamous cell carcinomaQ40401124
The absence of fucose but not the presence of galactose or bisecting N-acetylglucosamine of human IgG1 complex-type oligosaccharides shows the critical role of enhancing antibody-dependent cellular cytotoxicityQ40690773
Core fucosylation of IgG B cell receptor is required for antigen recognition and antibody productionQ41395654
Marketing approval of mogamulizumab: a triumph for glyco-engineeringQ42333453
Accelerating genome editing in CHO cells using CRISPR Cas9 and CRISPy, a web-based target finding toolQ43125635
The bioanalysis of the monoclonal antibody trastuzumab by high-performance liquid chromatography with fluorescence detection after immuno-affinity purification from human serum.Q45987354
Biacore surface matrix effects on the binding kinetics and affinity of an antigen/antibody complex.Q51538929
Kinetic analysis of monoclonal antibody-antigen interactions with a new biosensor based analytical systemQ58547271
Donor substrate binding and enzymatic mechanism of human core α1,6-fucosyltransferase (FUT8)Q84976737
P433issue7
P304page(s)801-808
P577publication date2017-04-18
P1433published inEngineering in Life SciencesQ15752832
P1476titleProducing defucosylated antibodies with enhanced in vitro antibody-dependent cellular cytotoxicity via FUT8 knockout CHO-S cells
P478volume17

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cites work (P2860)
Q64945488A cross-species whole genome siRNA screen in suspension-cultured Chinese hamster ovary cells identifies novel engineering targets.
Q92173365Small-molecule control of antibody N-glycosylation in engineered mammalian cells

Q93452631CHO-S FUT8-/-described by sourceP1343

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