Distinct nucleic acid interaction properties of HIV-1 nucleocapsid protein precursor NCp15 explain reduced viral infectivity

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Distinct nucleic acid interaction properties of HIV-1 nucleocapsid protein precursor NCp15 explain reduced viral infectivity is …
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scholarly articleQ13442814

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P356DOI10.1093/NAR/GKU335
P932PMC publication ID4066767
P698PubMed publication ID24813443
P5875ResearchGate publication ID262225279

P50authorRobert J GorelickQ87445459
Karin Musier-ForsythQ39700336
P2093author name stringWei Wang
Ioulia Rouzina
Mark C Williams
Zhengrong Wu
Jialin Li
Mithun Mitra
Nada Naiyer
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Rapid kinetics of protein-nucleic acid interaction is a major component of HIV-1 nucleocapsid protein's nucleic acid chaperone functionQ56893915
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Cleavage of HIV-1 gag polyprotein synthesized in vitro: sequential cleavage by the viral proteaseQ69564852
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Nucleic-acid-chaperone activity of retroviral nucleocapsid proteins: significance for viral replicationQ77362262
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Mapping the phase diagram of single DNA molecule force-induced melting in the presence of ethidiumQ81386671
Formation of immature and mature genomic RNA dimers in wild-type and protease-inactive HIV-1: Differential roles of the Gag polyprotein, nucleocapsid proteins NCp15, NCp9, NCp7, and the dimerization initiation siteQ85006185
Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 buddingQ24291784
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HIV-1 protease and reverse transcriptase control the architecture of their nucleocapsid partnerQ27302871
NMR structure of the HIV-1 nucleocapsid protein bound to stem-loop SL2 of the psi-RNA packaging signal. Implications for genome recognitionQ27625936
Elucidation of New Binding Interactions with the Human Tsg101 Protein Using Modified HIV-1 Gag-p6 Derived Peptide LigandsQ27666254
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Dynamical behavior of the HIV-1 nucleocapsid proteinQ27759385
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NMR View: A computer program for the visualization and analysis of NMR dataQ27860951
AIP1/ALIX Is a Binding Partner for HIV-1 p6 and EIAV p9 Functioning in Virus BuddingQ28204548
Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiencyQ28216587
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Gag mutations strongly contribute to HIV-1 resistance to protease inhibitors in highly drug-experienced patients besides compensating for fitness lossQ28474984
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Domain effects on the DNA-interactive properties of bacteriophage T4 gene 32 protein.Q30327404
Coupled integration of human immunodeficiency virus type 1 cDNA ends by purified integrase in vitro: stimulation by the viral nucleocapsid proteinQ30453495
The role of Pr55(gag) in the annealing of tRNA3Lys to human immunodeficiency virus type 1 genomic RNAQ30453508
The ubiquitous nature of RNA chaperone proteinsQ33184245
C-terminal domain modulates the nucleic acid chaperone activity of human T-cell leukemia virus type 1 nucleocapsid protein via an electrostatic mechanismQ33581258
The human immunodeficiency virus type 1 Gag polyprotein has nucleic acid chaperone activity: possible role in dimerization of genomic RNA and placement of tRNA on the primer binding siteQ33647840
Analysis of the assembly function of the human immunodeficiency virus type 1 gag protein nucleocapsid domain.Q33782263
Isolation of human immunodeficiency virus type 1 cores: retention of Vpr in the absence of p6(gag).Q33807166
Vpr-host interactions during HIV-1 viral life cycleQ33828332
Context-dependent phenotype of a human immunodeficiency virus type 1 nucleocapsid mutationQ33844733
Gag proteins of the highly replicative MN strain of human immunodeficiency virus type 1: posttranslational modifications, proteolytic processings, and complete amino acid sequencesQ33930053
Nucleocapsid protein of human immunodeficiency virus as a model protein with chaperoning functions and as a target for antiviral drugsQ34033167
p6Gag is required for particle production from full-length human immunodeficiency virus type 1 molecular clones expressing proteaseQ34288376
Theory of electrostatically regulated binding of T4 gene 32 protein to single- and double-stranded DNA.Q34351176
Use of terbium as a probe of tRNA tertiary structure and foldingQ34363183
Formation of a human immunodeficiency virus type 1 core of optimal stability is crucial for viral replicationQ34364648
The HIV-1 nucleocapsid zinc finger protein as a target of antiretroviral therapyQ34373424
Cofactors for human immunodeficiency virus type 1 cDNA integration in vitroQ34464329
Human immunodeficiency virus type 1 nucleocapsid zn(2+) fingers are required for efficient reverse transcription, initial integration processes, and protection of newly synthesized viral DNA.Q34464770
DNA condensation by multivalent cationsQ34468471
HIV-1 maturation inhibitor bevirimat stabilizes the immature Gag latticeQ34529834
Matrix domain modulates HIV-1 Gag's nucleic acid chaperone activity via inositol phosphate bindingQ34529887
Molecular basis of RNA recognition by the embryonic polarity determinant MEX-5.Q34607717
Features, processing states, and heterologous protein interactions in the modulation of the retroviral nucleocapsid protein functionQ34775654
Role of HIV-1 nucleocapsid protein in HIV-1 reverse transcriptionQ34775658
Complementary assays reveal a relationship between HIV-1 uncoating and reverse transcriptionQ35049239
The central globular domain of the nucleocapsid protein of human immunodeficiency virus type 1 is critical for virion structure and infectivityQ35834904
The p6gag domain of human immunodeficiency virus type 1 is sufficient for the incorporation of Vpr into heterologous viral particlesQ35838540
A leucine triplet repeat sequence (LXX)4 in p6gag is important for Vpr incorporation into human immunodeficiency virus type 1 particlesQ35850368
Determinants of the human immunodeficiency virus type 1 p15NC-RNA interaction that affect enhanced cleavage by the viral protease.Q35889311
Human immunodeficiency virus type 1 nucleocapsid protein specifically stimulates Mg2+-dependent DNA integration in vitroQ35890609
Force spectroscopy reveals the DNA structural dynamics that govern the slow binding of Actinomycin DQ36008084
Mutational analysis of the C-terminal gag cleavage sites in human immunodeficiency virus type 1Q36098898
Nucleic acid chaperone activity of HIV-1 nucleocapsid protein: critical role in reverse transcription and molecular mechanismQ36258296
Functional redundancy in HIV-1 viral particle assemblyQ36397587
Inhibition of reverse transcriptase activity increases stability of the HIV-1 coreQ36506926
Cleavage of p15 protein in vitro by human immunodeficiency virus type 1 protease is RNA dependentQ36635813
The activity of the protease of human immunodeficiency virus type 1 is initiated at the membrane of infected cells before the release of viral proteins and is required for release to occur with maximum efficiency.Q36636632
Partial inhibition of the human immunodeficiency virus type 1 protease results in aberrant virus assembly and the formation of noninfectious particles.Q36650046
Mapping of functionally important residues of a cysteine-histidine box in the human immunodeficiency virus type 1 nucleocapsid proteinQ36653772
Incorporation of Vpr into human immunodeficiency virus type 1 virions: requirement for the p6 region of gag and mutational analysis.Q36655127
Mutations of RNA and protein sequences involved in human immunodeficiency virus type 1 packaging result in production of noninfectious virusQ36804927
Noninfectious human immunodeficiency virus type 1 mutants deficient in genomic RNA.Q36809360
HIV-1 inactivation by 4-vinylpyridine is enhanced by dissociating Zn(2+) from nucleocapsid proteinQ36910007
Retroviral nucleocapsid proteins display nonequivalent levels of nucleic acid chaperone activityQ36933996
Beyond Tsg101: the role of Alix in 'ESCRTing' HIV-1.Q36992975
Targeting the viral nucleocapsid protein in anti-HIV-1 therapy.Q37066805
Strand transfer events during HIV-1 reverse transcriptionQ37086417
Aromatic residue mutations reveal direct correlation between HIV-1 nucleocapsid protein's nucleic acid chaperone activity and retroviral replicationQ37099404
RNA chaperones, RNA annealers and RNA helicasesQ37112437
Optical tweezers experiments resolve distinct modes of DNA-protein binding.Q37378660
HIV-1 nucleocapsid protein induces "maturation" of dimeric retroviral RNA in vitroQ37427179
HIV-1 Gag processing intermediates trans-dominantly interfere with HIV-1 infectivity.Q37447762
Human immunodeficiency virus type 1 Vpr: functions and molecular interactionsQ37490614
Properties and functions of the nucleocapsid protein in virus assemblyQ37820816
Targeting protein-protein and protein-nucleic acid interactions for anti-HIV therapyQ37923526
HIV-1 nucleocapsid protein activates transient melting of least stable parts of the secondary structure of TAR and its complementary sequenceQ38290651
Mechanistic insights into the kinetics of HIV-1 nucleocapsid protein-facilitated tRNA annealing to the primer binding siteQ38343425
On the thermodynamics and kinetics of the cooperative binding of bacteriophage T4-coded gene 32 (helix destabilizing) protein to nucleic acid latticesQ38356714
Sequential steps in human immunodeficiency virus particle maturation revealed by alterations of individual Gag polyprotein cleavage sites.Q39579012
Biochemical and structural analysis of isolated mature cores of human immunodeficiency virus type 1.Q39588178
The relationship between HIV-1 genome RNA dimerization, virion maturation and infectivityQ39615571
The p2 domain of human immunodeficiency virus type 1 Gag regulates sequential proteolytic processing and is required to produce fully infectious virions.Q40042979
Critical role of helix 4 of HIV-1 capsid C-terminal domain in interactions with human lysyl-tRNA synthetaseQ40179797
Conformational behaviour of the active and inactive forms of the nucleocapsid NCp7 of HIV-1 studied by 1H NMR.Q41495032
Modulation of T4 gene 32 protein DNA binding activity by the recombination mediator protein UvsY.Q42119976
HIV-1 p6 - a structured to flexible multifunctional membrane-interacting protein.Q42285526
Distinct binding interactions of HIV-1 Gag to Psi and non-Psi RNAs: implications for viral genomic RNA packaging.Q42288399
A strongly transdominant mutation in the human immunodeficiency virus type 1 gag gene defines an Achilles heel in the virus life cycleQ42546287
Role of distal zinc finger of nucleocapsid protein in genomic RNA dimerization of human immunodeficiency virus type 1; no role for the palindrome crowning the R-U5 hairpinQ42643768
Molecular mimicry of human tRNALys anti-codon domain by HIV-1 RNA genome facilitates tRNA primer annealingQ42703988
HIV-1 nucleocapsid protein zinc finger structures induce tRNA(Lys,3) structural changes but are not critical for primer/template annealingQ44253743
Ordered aggregation of ribonucleic acids by the human immunodeficiency virus type 1 nucleocapsid protein.Q44821597
Salt dependent binding of T4 gene 32 protein to single and double-stranded DNA: single molecule force spectroscopy measurementsQ44823457
Solution structure of the human immunodeficiency virus type 1 p6 proteinQ44981187
Properties and growth mechanism of the ordered aggregation of a model RNA by the HIV-1 nucleocapsid protein: an electron microscopy investigationQ44987232
P275copyright licenseCreative Commons Attribution 3.0 UnportedQ14947546
P6216copyright statuscopyrightedQ50423863
P433issue11
P407language of work or nameEnglishQ1860
P921main subjectinfectivityQ1662346
viral nucleocapsidQ4384107
P304page(s)7145-7159
P577publication date2014-05-09
P1433published inNucleic Acids ResearchQ135122
P1476titleDistinct nucleic acid interaction properties of HIV-1 nucleocapsid protein precursor NCp15 explain reduced viral infectivity
P478volume42

Reverse relations

cites work (P2860)
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Q36286511The thermodynamics of Pr55Gag-RNA interaction regulate the assembly of HIV.

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