Directed evolution of APEX2 for electron microscopy and proximity labeling.

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Directed evolution of APEX2 for electron microscopy and proximity labeling. is …
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scholarly articleQ13442814

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P6179Dimensions Publication ID1033631778
P356DOI10.1038/NMETH.3179
P932PMC publication ID4296904
P698PubMed publication ID25419960
P5875ResearchGate publication ID268787696

P50authorVamsi MoothaQ7913059
Kimberli J KamerQ41679004
P2093author name stringMark H Ellisman
Jeffrey D Martell
Alice Y Ting
Thomas J Deerinck
Stephanie S Lam
P2860cites workKinetic study of the inactivation of ascorbate peroxidase by hydrogen peroxide.Q41975804
MICU1 controls both the threshold and cooperative activation of the mitochondrial Ca²⁺ uniporterQ41976396
Imaging trans-cellular neurexin-neuroligin interactions by enzymatic probe ligationQ42129736
Substrate binding and catalytic mechanism in ascorbate peroxidase: evidence for two ascorbate binding sitesQ44212912
Optimisation of expression and immobilized metal ion affinity chromatographic purification of recombinant (His)6-tagged cytochrome P450 hydroperoxide lyase in Escherichia coliQ44371689
Crystal structure of the ascorbate peroxidase-salicylhydroxamic acid complexQ44965677
Role of electrostatics and salt bridges in stabilizing the compound I radical in ascorbate peroxidaseQ46597983
Correlative microscopy and electron tomography of GFP through photooxidationQ46797423
Engineering antibody affinity by yeast surface display.Q51636979
Delta-aminolevulinate increases heme saturation and yield of human cystathionine beta-synthase expressed in Escherichia coli.Q54618916
Inactivation of peroxidase by hydrogen peroxide and its protection by a reductant agentQ68736592
Simultaneous determination of hemes a, b, and c from pyridine hemochrome spectraQ69010759
Which of the commonly used marker enzymes gives the best results in colorimetric and fluorimetric enzyme immunoassays: horseradish peroxidase, alkaline phosphatase or beta-galactosidase?Q69946829
MICU1 encodes a mitochondrial EF hand protein required for Ca(2+) uptakeQ24294670
MICU1 is an essential gatekeeper for MCU-mediated mitochondrial Ca(2+) uptake that regulates cell survivalQ24301822
Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acidsQ24306330
Integrative genomics identifies MCU as an essential component of the mitochondrial calcium uniporterQ24306825
A forty-kilodalton protein of the inner membrane is the mitochondrial calcium uniporterQ24306850
A mitochondrial protein compendium elucidates complex I disease biologyQ24655079
Photooxidation-guided ultrastructural identification and analysis of cells in neuronal tissue labeled with green fluorescent proteinQ27318307
A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organismsQ27321551
Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3Q27861127
MICU2, a paralog of MICU1, resides within the mitochondrial uniporter complex to regulate calcium handlingQ28486060
Identification of a signal for rapid export of proteins from the nucleusQ29547742
Isolating and engineering human antibodies using yeast surface displayQ33280948
Chimeric molecules employing horseradish peroxidase as reporter enzyme for protein localization in the electron microscope.Q34068025
Multicolor and electron microscopic imaging of connexin traffickingQ34124663
A transcription activator-like effector toolbox for genome engineeringQ34244995
Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopyQ34307392
Outer mitochondrial membrane permeability can regulate coupled respiration and cell survivalQ35690379
The role of quaternary interactions on the stability and activity of ascorbate peroxidaseQ36280660
Proteomic mapping of the human mitochondrial intermembrane space in live cells via ratiometric APEX tagging.Q36550699
Mobility of cytochrome P450 in the endoplasmic reticulum membraneQ36754894
MICU1 motifs define mitochondrial calcium uniporter binding and activity.Q37574309
MICU1 and MICU2 play nonredundant roles in the regulation of the mitochondrial calcium uniporterQ37707538
Membrane targeted horseradish peroxidase as a marker for correlative fluorescence and electron microscopy studies.Q39384781
Succinylacetone, a potent inhibitor of heme biosynthesis: Effect on cell growth, heme content and δ-aminolevulinic acid dehydratase activity of malignant murine erythroleukemia cellsQ40608672
Characterization of neuronal nitric oxide synthase and a C415H mutant, purified from a baculovirus overexpression systemQ41411645
Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging.Q41927329
P433issue1
P921main subjectdirected evolutionQ440681
microscopyQ1074953
P304page(s)51-54
P577publication date2014-11-24
P1433published inNature MethodsQ680640
P1476titleDirected evolution of APEX2 for electron microscopy and proximity labeling
P478volume12

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