A sensitive assay using a native protein substrate for screening HIV-1 maturation inhibitors targeting the protease cleavage site between the matrix and capsid

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A sensitive assay using a native protein substrate for screening HIV-1 maturation inhibitors targeting the protease cleavage site between the matrix and capsid is …
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scholarly articleQ13442814

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P356DOI10.1021/BI4005232
P932PMC publication ID3855639
P698PubMed publication ID23763575
P5875ResearchGate publication ID239945145

P50authorRonald SwanstromQ62641484
P2093author name stringCelia A Schiffer
Sook-Kyung Lee
Nancy Cheng
William Janzen
Marc Potempa
Emily Hull-Ryde
P2860cites workThe prototype HIV-1 maturation inhibitor, bevirimat, binds to the CA-SP1 cleavage site in immature Gag particlesQ21245046
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Q25938983
Statistical methods for assessing agreement between two methods of clinical measurementQ26778461
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X-ray crystallographic structure of ABT-378 (lopinavir) bound to HIV-1 proteaseQ27639157
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Novel binding mode of highly potent HIV-proteinase inhibitors incorporating the (R)-hydroxyethylamine isostereQ27656274
HIV Capsid is a Tractable Target for Small Molecule Therapeutic InterventionQ27666355
Distinct Effects of Two HIV-1 Capsid Assembly Inhibitor Families That Bind the Same Site within the N-Terminal Domain of the Viral CA ProteinQ27678471
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The Mechanism of HIV-1 Core Assembly: Insights from Three-Dimensional Reconstructions of Authentic VirionsQ27702100
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Mutations in multiple domains of Gag drive the emergence of in vitro resistance to the phosphonate-containing HIV-1 protease inhibitor GS-8374.Q36506829
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PA-457: A potent HIV inhibitor that disrupts core condensation by targeting a late step in Gag processingQ37089562
Resilience to resistance of HIV-1 protease inhibitors: profile of darunavir.Q37235740
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HIV-1 Gag processing intermediates trans-dominantly interfere with HIV-1 infectivity.Q37447762
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HIV-1 assembly, budding, and maturationQ38023864
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Three residues in HIV-1 matrix contribute to protease inhibitor susceptibility and replication capacityQ39621109
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Transmission fitness of drug-resistant human immunodeficiency virus and the prevalence of resistance in the antiretroviral-treated populationQ39642550
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Functional Surfaces of the Human Immunodeficiency Virus Type 1 Capsid ProteinQ39748566
The p2 domain of human immunodeficiency virus type 1 Gag regulates sequential proteolytic processing and is required to produce fully infectious virions.Q40042979
Mutant murine leukemia virus Gag proteins lacking proline at the N-terminus of the capsid domain block infectivity in virions containing wild-type Gag.Q40326740
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Proteolytic refolding of the HIV-1 capsid protein amino-terminus facilitates viral core assemblyQ41047831
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Anti-AIDS agents, 11. Betulinic acid and platanic acid as anti-HIV principles from Syzigium claviflorum, and the anti-HIV activity of structurally related triterpenoidsQ42285617
3-O-(3',3'-dimethysuccinyl) betulinic acid inhibits maturation of the human immunodeficiency virus type 1 Gag precursor assembled in vitroQ42424208
A strongly transdominant mutation in the human immunodeficiency virus type 1 gag gene defines an Achilles heel in the virus life cycleQ42546287
Betulinic acid and dihydrobetulinic acid derivatives as potent anti-HIV agentsQ42555749
A synthetic peptide from HIV-1 gp41 is a potent inhibitor of virus-mediated cell-cell fusionQ42560226
A cleavage enzyme-cytometric bead array provides biochemical profiling of resistance mutations in HIV-1 Gag and proteaseQ42872702
High prevalence of natural polymorphisms in Gag (CA-SP1) associated with reduced response to Bevirimat, an HIV-1 maturation inhibitorQ43227082
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Antiviral inhibition of the HIV-1 capsid proteinQ44380725
The HIV-1 capsid protein C-terminal domain in complex with a virus assembly inhibitorQ45432280
Fluorescence anisotropy assay for proteolysis of specifically labeled fusion proteins.Q54494902
Measurement of specific protease activity utilizing fluorescence polarizationQ73286573
Implications for viral capsid assembly from crystal structures of HIV-1 Gag(1-278) and CA(N)(133-278)Q80280623
P4510describes a project that usesImageQuantQ112270642
P433issue29
P407language of work or nameEnglishQ1860
P304page(s)4929-4940
P577publication date2013-07-11
P1433published inBiochemistryQ764876
P1476titleA sensitive assay using a native protein substrate for screening HIV-1 maturation inhibitors targeting the protease cleavage site between the matrix and capsid
P478volume52

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cites work (P2860)
Q34435796Development of a Novel Screening Strategy Designed to Discover a New Class of HIV Drugs
Q41467248Fluorescence polarization assays in high-throughput screening and drug discovery: a review
Q58597952HIV-1 Protease Uses Bi-Specific S2/S2' Subsites to Optimize Cleavage of Two Classes of Target Sites
Q92233282Methods to analyze STUbL activity
Q39294730Recent developments in protease activity assays and sensors.
Q38818907Stapled peptides: providing the best of both worlds in drug development

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