Directed evolution of a yeast-displayed HIV-1 SOSIP gp140 spike protein toward improved expression and affinity for conformational antibodies

scientific article

Directed evolution of a yeast-displayed HIV-1 SOSIP gp140 spike protein toward improved expression and affinity for conformational antibodies is …
instance of (P31):
scholarly articleQ13442814

External links are
P819ADS bibcode2015PLoSO..1017227G
P356DOI10.1371/JOURNAL.PONE.0117227
P932PMC publication ID4331506
P698PubMed publication ID25688555
P5875ResearchGate publication ID272511951

P50authorMargaret E. AckermanQ57012155
Michael B BattlesQ47115798
P2093author name stringSebastian K Grimm
P2860cites workFew and far between: how HIV may be evading antibody avidityQ21090507
Gene Designer: a synthetic biology tool for constructing artificial DNA segmentsQ21284253
The HIV-1 Envelope Glycoproteins: Fusogens, Antigens, and ImmunogensQ22242262
Comprehensive cross-clade neutralization analysis of a panel of anti-human immunodeficiency virus type 1 monoclonal antibodiesQ24561905
DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolutionQ24562755
Access of antibody molecules to the conserved coreceptor binding site on glycoprotein gp120 is sterically restricted on primary human immunodeficiency virus type 1Q24645530
Antibody Recognition and Neutralization Determinants on Domains I and II of West Nile Virus Envelope ProteinQ27477718
A fusion-intermediate state of HIV-1 gp41 targeted by broadly neutralizing antibodiesQ27485578
Co-evolution of a broadly neutralizing HIV-1 antibody and founder virusQ27644501
Developmental pathway for potent V1V2-directed HIV-neutralizing antibodiesQ27644515
Structural Basis of Immune Evasion at the Site of CD4 Attachment on HIV-1 gp120Q27658449
Computational design of epitope-scaffolds allows induction of antibodies specific for a poorly immunogenic HIV vaccine epitopeQ27664365
Elicitation of structure-specific antibodies by epitope scaffoldsQ27664711
Sequence and Structural Convergence of Broad and Potent HIV Antibodies That Mimic CD4 BindingQ27670829
Focused Evolution of HIV-1 Neutralizing Antibodies Revealed by Structures and Deep SequencingQ27671696
Computation-guided backbone grafting of a discontinuous motif onto a protein scaffoldQ27675190
Directed evolution of the epidermal growth factor receptor extracellular domain for expression in yeast.Q51304377
Yeast surface display of a noncovalent MHC class II heterodimer complexed with antigenic peptide.Q51356858
Yeast polypeptide fusion surface display levels predict thermal stability and soluble secretion efficiencyQ56902099
Direct random mutagenesis of gene-sized DNA fragments using polymerase chain reactionQ72141004
Secretion-and-capture cell-surface display for selection of target-binding proteinsQ83608801
Rational HIV Immunogen Design to Target Specific Germline B Cell ReceptorsQ27677104
Crystal Structure of a Soluble Cleaved HIV-1 Envelope TrimerQ27680496
Cryo-EM Structure of a Fully Glycosylated Soluble Cleaved HIV-1 Envelope TrimerQ27680497
Structural delineation of a quaternary, cleavage-dependent epitope at the gp41-gp120 interface on intact HIV-1 Env trimersQ27683553
Fine mapping of the interaction of neutralizing and nonneutralizing monoclonal antibodies with the CD4 binding site of human immunodeficiency virus type 1 gp120.Q28217208
DNA shuffling of a family of genes from diverse species accelerates directed evolutionQ28259708
A next-generation cleaved, soluble HIV-1 Env trimer, BG505 SOSIP.664 gp140, expresses multiple epitopes for broadly neutralizing but not non-neutralizing antibodiesQ28299280
Antibody neutralization and escape by HIV-1Q29547345
Broad and potent neutralizing antibodies from an African donor reveal a new HIV-1 vaccine targetQ29547347
Broad neutralization coverage of HIV by multiple highly potent antibodiesQ29615361
Efficient neutralization of primary isolates of HIV-1 by a recombinant human monoclonal antibodyQ29618366
Rapid evolution of the neutralizing antibody response to HIV type 1 infectionQ29618603
Rational design of envelope identifies broadly neutralizing human monoclonal antibodies to HIV-1Q29619511
Human monoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1Q29619512
Fine epitope mapping of monoclonal antibodies against hemagglutinin of a highly pathogenic H5N1 influenza virus using yeast surface displayQ30402648
A flow cytometric assay for screening improved heterologous protein secretion in yeastQ30820875
Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinityQ30923970
Production of complex human glycoproteins in yeastQ31155283
Yeast surface display for directed evolution of protein expression, affinity, and stabilityQ31826513
An episomal expression vector for screening mutant gene libraries in Pichia pastorisQ33215505
Isolating and engineering human antibodies using yeast surface displayQ33280948
Picomolar affinity fibronectin domains engineered utilizing loop length diversity, recursive mutagenesis, and loop shufflingQ33349284
Antibody binding site mapping of SARS-CoV spike protein receptor-binding domain by a combination of yeast surface display and phage peptide library screeningQ33516972
Analysis of memory B cell responses and isolation of novel monoclonal antibodies with neutralizing breadth from HIV-1-infected individualsQ33526340
Characteristics of the earliest cross-neutralizing antibody response to HIV-1.Q33798076
Rational antibody-based HIV-1 vaccine design: current approaches and future directionsQ33937285
Medicine. The need for a global HIV vaccine enterpriseQ34209402
B-cell-lineage immunogen design in vaccine development with HIV-1 as a case studyQ34273373
Stabilization of the soluble, cleaved, trimeric form of the envelope glycoprotein complex of human immunodeficiency virus type 1.Q34361680
Rapid conformational epitope mapping of anti-gp120 antibodies with a designed mutant panel displayed on yeastQ34480254
Induction of immunity to human immunodeficiency virus type-1 by vaccinationQ34540645
Humanization of yeast to produce complex terminally sialylated glycoproteinsQ34564538
Increased functional stability and homogeneity of viral envelope spikes through directed evolutionQ34611144
Protein folding and quality control in the endoplasmic reticulum: Recent lessons from yeast and mammalian cell systemsQ35160159
Structure of a high-affinity "mimotope" peptide bound to HIV-1-neutralizing antibody b12 explains its inability to elicit gp120 cross-reactive antibodiesQ36016347
HIV-1 envelope trimer elicits more potent neutralizing antibody responses than monomeric gp120.Q36132728
Evolution of an HIV glycan-dependent broadly neutralizing antibody epitope through immune escapeQ36388754
Engineering HIV envelope protein to activate germline B cell receptors of broadly neutralizing anti-CD4 binding site antibodiesQ36750045
Yeast surface display for screening combinatorial polypeptide librariesQ36865126
Highly L and D enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection methodQ36976667
Polyreactivity increases the apparent affinity of anti-HIV antibodies by heteroligationQ36977531
Human immunodeficiency virus type 1 env trimer immunization of macaques and impact of priming with viral vector or stabilized core protein.Q37033291
An engineered mutant of HIV-1 gp120 formulated with adjuvant Quil A promotes elicitation of antibody responses overlapping the CD4-binding siteQ37069430
Cleavage strongly influences whether soluble HIV-1 envelope glycoprotein trimers adopt a native-like conformationQ37318042
Toward an antibody-based HIV-1 vaccineQ37613695
Challenges for structure-based HIV vaccine designQ37667763
Correlates of protection induced by vaccinationQ37750121
Engineering of glycosylation in yeast and other fungi: current state and perspectivesQ37768357
Limitations to the structure-based design of HIV-1 vaccine immunogensQ37910841
Emerging concepts on the role of innate immunity in the prevention and control of HIV infectionQ37955261
Vaccine design: emerging concepts and renewed optimismQ38087785
Neutralizing monoclonal antibodies against hepatitis C virus E2 protein bind discontinuous epitopes and inhibit infection at a postattachment stepQ38335402
Mapping the journey to an HIV vaccineQ38565206
Broadly neutralizing HIV antibodies define a glycan-dependent epitope on the prefusion conformation of gp41 on cleaved envelope trimersQ39001126
Comparative evaluation of trimeric envelope glycoproteins derived from subtype C and B HIV-1 R5 isolatesQ39484948
A recombinant human immunodeficiency virus type 1 envelope glycoprotein complex stabilized by an intermolecular disulfide bond between the gp120 and gp41 subunits is an antigenic mimic of the trimeric virion-associated structureQ39588870
Genotype-specific neutralization and protection by antibodies against dengue virus type 3.Q39668466
Oligomeric and conformational properties of a proteolytically mature, disulfide-stabilized human immunodeficiency virus type 1 gp140 envelope glycoproteinQ39684388
Role of complex carbohydrates in human immunodeficiency virus type 1 infection and resistance to antibody neutralizationQ39723388
Cardiolipin polyspecific autoreactivity in two broadly neutralizing HIV-1 antibodies.Q40429324
A potent cross-clade neutralizing human monoclonal antibody against a novel epitope on gp41 of human immunodeficiency virus type 1.Q40757568
Improved design of an antigen with enhanced specificity for the broadly HIV-neutralizing antibody b12.Q45149860
Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technologyQ46170507
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P433issue2
P407language of work or nameEnglishQ1860
P921main subjectdirected evolutionQ440681
protein evolutionQ59870539
P304page(s)e0117227
P577publication date2015-02-17
P1433published inPLOS OneQ564954
P1476titleDirected evolution of a yeast-displayed HIV-1 SOSIP gp140 spike protein toward improved expression and affinity for conformational antibodies
P478volume10

Reverse relations

cites work (P2860)
Q47436657Development of unique cytotoxic chimeric antigen receptors based on human scFv targeting B7H6.
Q47765958Directed Evolution of Glycopeptides Using mRNA Display
Q58601813Display of the HIV envelope protein at the yeast cell surface for immunogen development
Q90024145Identification of variant HIV envelope proteins with enhanced affinities for precursors to anti-gp41 broadly neutralizing antibodies
Q55518743Towards conformational fidelity of a quaternary HIV-1 epitope: computational design and directed evolution of a minimal V1V2 antigen.

Search more.