Polar localization of PhoN2, a periplasmic virulence-associated factor of Shigella flexneri, is required for proper IcsA exposition at the old bacterial pole

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Polar localization of PhoN2, a periplasmic virulence-associated factor of Shigella flexneri, is required for proper IcsA exposition at the old bacterial pole is …
instance of (P31):
scholarly articleQ13442814

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P819ADS bibcode2014PLoSO...990230S
P356DOI10.1371/JOURNAL.PONE.0090230
P932PMC publication ID3937361
P698PubMed publication ID24587292
P5875ResearchGate publication ID260448479

P50authorLucia NencioniQ56855041
Daniela ScribanoQ86080907
P2093author name stringFabio Polticelli
Cecilia Ambrosi
Mauro Nicoletti
Monica Pompili
Andrea Petrucca
Gianni Prosseda
Mariassunta Casalino
Carlo Zagaglia
Elena Bruni
P2860cites workA novel proline-rich motif present in ActA of Listeria monocytogenes and cytoskeletal proteins is the ligand for the EVH1 domain, a protein module present in the Ena/VASP familyQ24532768
Neural Wiskott-Aldrich syndrome protein is implicated in the actin-based motility of Shigella flexneriQ24533240
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Q25938983
X-ray structures of a novel acid phosphatase from Escherichia blattae and its complex with the transition-state analog molybdateQ27622688
Structure and mutational analysis of the PhoN protein of Salmonella typhimurium provide insight into mechanistic detailsQ27643702
Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoterQ27860697
The type III secretion injectisomeQ29617944
Lipopolysaccharide O antigen chains mask IcsA (VirG) in Shigella flexneriQ73341660
The unipolar Shigella surface protein IcsA is targeted directly to the bacterial old pole: IcsP cleavage of IcsA occurs over the entire bacterial surfaceQ77410714
Analysis of virulence plasmid gene expression defines three classes of effectors in the type III secretion system of Shigella flexneriQ81503204
Apyrases (ATP diphosphohydrolases, EC 3.6.1.5): function and relationship to ATPasesQ40976689
The making of a gradient: IcsA (VirG) polarity in Shigella flexneriQ41475084
Polar localization of the autotransporter family of large bacterial virulence proteinsQ41668645
Apyrase, the product of the virulence plasmid-encoded phoN2 (apy) gene of Shigella flexneri, is necessary for proper unipolar IcsA localization and for efficient intercellular spread.Q42069451
Outer membrane protein A of Escherichia coli forms temperature-sensitive channels in planar lipid bilayersQ42614221
The virulence plasmid pWR100 and the repertoire of proteins secreted by the type III secretion apparatus of Shigella flexneriQ42638971
Molecular evolution of the nicotinic acid requirement within the Shigella/EIEC pathotype.Q44245939
Conserved proline residues near the N-terminus are important for enzymatic activity of class A bacterial acid phosphatasesQ44251458
Ala160 and His116 residues are involved in activity and specificity of apyrase, an ATP-hydrolysing enzyme produced by enteroinvasive Escherichia coli.Q46693690
IcsA, a polarly localized autotransporter with an atypical signal peptide, uses the Sec apparatus for secretion, although the Sec apparatus is circumferentially distributedQ47608547
SopA, the outer membrane protease responsible for polar localization of IcsA in Shigella flexneriQ48053197
Enteroinvasive Escherichia coli virulence-plasmid-carried apyrase (apy) and ospB genes are organized as a bicistronic operon and are subject to differential expressionQ48287842
Shigella apyrase--a novel variant of bacterial acid phosphatases?Q50760235
Decrease by psychotropic drugs and local anaesthetics of membrane fluidity measured by fluorescence anisotropy in Escherichia coliQ51678868
Disruption of IcsP, the major Shigella protease that cleaves IcsA, accelerates actin-based motility.Q54560755
In vitro insertion and assembly of outer membrane protein PhoE of Escherichia coli K-12 into the outer membrane. Role of Triton X-100.Q54588208
The importance of being proline: the interaction of proline-rich motifs in signaling proteins with their cognate domainsQ29618469
Interaction of an autotransporter passenger domain with BamA during its translocation across the bacterial outer membrane.Q30157080
Membrane protein architects: the role of the BAM complex in outer membrane protein assembly.Q30157374
Contribution of the periplasmic chaperone Skp to efficient presentation of the autotransporter IcsA on the surface of Shigella flexneriQ30157451
Kinetics of folding of Escherichia coli OmpA from narrow to large pore conformation in a planar bilayerQ30160259
Secondary structure of the outer membrane proteins OmpA of Escherichia coli and OprF of Pseudomonas aeruginosaQ30176815
Membrane Assembly of Circularly Permuted Variants of theE. coliOuter Membrane Protein OmpAQ30193246
Type V protein secretion pathway: the autotransporter storyQ33559645
Identification of icsA, a plasmid locus of Shigella flexneri that governs bacterial intra- and intercellular spread through interaction with F-actinQ33859430
Polar targeting of Shigella virulence factor IcsA in Enterobacteriacae and VibrioQ33934139
Epitope tagging of chromosomal genes in SalmonellaQ33953109
Molecular basis of the intracellular spreading of ShigellaQ34090958
Actin-based motility of pathogens: the Arp2/3 complex is a central playerQ34156259
Genome sequence of Shigella flexneri serotype 5a strain M90T SmQ34267669
Bacterial invasion: the paradigms of enteroinvasive pathogensQ34312229
Outer membrane protein A (OmpA): a new player in shigella flexneri protrusion formation and inter-cellular spreadingQ34482609
A molecular Swiss army knife: OmpA structure, function and expressionQ34636047
E. coli transports aggregated proteins to the poles by a specific and energy-dependent processQ34992028
Lack of cleavage of IcsA in Shigella flexneri causes aberrant movement and allows demonstration of a cross-reactive eukaryotic proteinQ35468115
SurA assists the folding of Escherichia coli outer membrane proteinsQ35604222
Outer membrane protein A and OprF: versatile roles in Gram-negative bacterial infectionsQ35918591
IcsA surface presentation in Shigella flexneri requires the periplasmic chaperones DegP, Skp, and SurA.Q35949250
Molecular pathogenesis of Shigella spp.: controlling host cell signaling, invasion, and death by type III secretion.Q36422508
Mutagenesis of the Shigella flexneri autotransporter IcsA reveals novel functional regions involved in IcsA biogenesis and recruitment of host neural Wiscott-Aldrich syndrome proteinQ36747291
OmpA: gating and dynamics via molecular dynamics simulationsQ36865154
Decay of mRNA in Escherichia coli: investigation of the fate of specific segments of transcriptsQ37602567
A multifactor regulatory circuit involving H-NS, VirF and an antisense RNA modulates transcription of the virulence gene icsA of Shigella flexneri.Q38333799
Bacterial nonspecific acid phosphohydrolases: physiology, evolution and use as tools in microbial biotechnology.Q38549832
Isolation, cloning, and expression of an acid phosphatase containing phosphotyrosyl phosphatase activity from Prevotella intermedia.Q39498038
Expression of the virulence plasmid-carried apyrase gene (apy) of enteroinvasive Escherichia coli and Shigella flexneri is under the control of H-NS and the VirF and VirB regulatory cascade.Q39573310
Secretion of Ipa proteins by Shigella flexneri: inducer molecules and kinetics of activation.Q39831093
Unipolar localization and ATPase activity of IcsA, a Shigella flexneri protein involved in intracellular movementQ39927930
Regulation of surface presentation of IcsA, a Shigella protein essential to intracellular movement and spread, is growth phase dependent.Q40376661
Genetic modulation of Shigella flexneri 2a lipopolysaccharide O antigen modal chain length reveals that it has been optimized for virulence.Q40657135
The secretion of the Shigella flexneri Ipa invasins is activated by epithelial cells and controlled by IpaB and IpaD.Q40793797
Major Proteins of the Escherichia coli Outer Cell Envelope Membrane. Interaction of Protein II* with LipopolysaccharideQ40846831
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P433issue2
P407language of work or nameEnglishQ1860
P921main subjectShigella flexneriQ1644417
P304page(s)e90230
P577publication date2014-02-27
P1433published inPLOS OneQ564954
P1476titlePolar localization of PhoN2, a periplasmic virulence-associated factor of Shigella flexneri, is required for proper IcsA exposition at the old bacterial pole
P478volume9

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Q41682716The Shigella flexneri OmpA amino acid residues 188EVQ190 are essential for the interaction with the virulence factor PhoN2.

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