Nanobody Technology: A Versatile Toolkit for Microscopic Imaging, Protein-Protein Interaction Analysis, and Protein Function Exploration.

scientific article

Nanobody Technology: A Versatile Toolkit for Microscopic Imaging, Protein-Protein Interaction Analysis, and Protein Function Exploration. is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.3389/FIMMU.2017.00771
P932PMC publication ID5495861
P698PubMed publication ID28725224

P50authorEls BegheinQ59553989
P2093author name stringJan Gettemans
P2860cites workProteomic analysis of rat liver peroxisome: presence of peroxisome-specific isozyme of Lon proteaseQ24297876
Adaptor protein self-assembly drives the control of a cullin-RING ubiquitin ligaseQ24336527
Structure of a nanobody-stabilized active state of the β(2) adrenoceptorQ24597139
Visualization and targeted disruption of protein interactions in living cellsQ24599716
An affinity-directed protein missile system for targeted proteolysis.Q41643543
Site-specific labeling of cysteine-tagged camelid single-domain antibody-fragments for use in molecular imagingQ43432020
Reversible site-selective labeling of membrane proteins in live cellsQ44807774
TEV protease-mediated cleavage in Drosophila as a tool to analyze protein functions in living organismsQ47710950
Receptor-mediated sorting of soluble vacuolar proteins ends at the trans-Golgi network/early endosomeQ48042401
Engineered Upconversion Nanoparticles for Resolving Protein Interactions inside Living Cells.Q48055032
Single-molecule microscopy of molecules tagged with GFP or RFP derivatives in mammalian cells using nanobody binders.Q48663381
Singlet Oxygen-Induced Furan Oxidation for Site-Specific and Chemoselective Peptide LigationQ49054101
Fluorescent fusion protein knockout mediated by anti-GFP nanobodyQ50506348
Development by Genetic Immunization of Monovalent Antibodies (Nanobodies) Behaving as Antagonists of the Human ChemR23 ReceptorQ51529766
Highly specific and potently activating Vγ9Vδ2-T cell specific nanobodies for diagnostic and therapeutic applications.Q51666357
Isomerization and Oxidation in the Complementarity-Determining Regions of a Monoclonal Antibody: A Study of the Modification-Structure-Function Correlations by Hydrogen-Deuterium Exchange Mass SpectrometryQ51739313
Pex5p Imports Folded Tetrameric Catalase by Interaction with Pex13pQ51758002
Eci1p uses a PTS1 to enter peroxisomes: either its own or that of a partner, Dci1pQ52542796
Super-resolution fluorescence microscopyQ24630861
Multicolor super-resolution imaging with photo-switchable fluorescent probesQ24642794
Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopyQ24642820
Yeast two-hybrid: State of the artQ24796037
Nanobodies as Versatile Tools to Understand, Diagnose, Visualize and Treat CancerQ26739702
Monitoring interactions and dynamics of endogenous beta-catenin with intracellular nanobodies in living cellsQ27311697
Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidinQ27330372
A llama-derived gelsolin single-domain antibody blocks gelsolin-G-actin interactionQ27659637
Structure and properties of a complex of α-synuclein and a single-domain camelid antibodyQ27663303
Defining a protective epitope on factor H binding protein, a key meningococcal virulence factor and vaccine antigenQ27676318
Crystal structure of a camel single-domain VH antibody fragment in complex with lysozymeQ27733338
Selection and identification of single domain antibody fragments from camel heavy-chain antibodiesQ28250517
A simple, versatile method for GFP-based super-resolution microscopy via nanobodiesQ28265564
Naturally occurring antibodies devoid of light chainsQ28269589
Targeting and tracing antigens in live cells with fluorescent nanobodiesQ28270182
Nanobodies: Natural Single-Domain AntibodiesQ28287268
Hitchhiking of Cu/Zn superoxide dismutase to peroxisomes--evidence for a natural piggyback import mechanism in mammalsQ28571100
Proteomics characterization of mouse kidney peroxisomes by tandem mass spectrometry and protein correlation profilingQ28591793
Inhibitory cortactin nanobodies delineate the role of NTA- and SH3-domain-specific functions during invadopodium formation and cancer cell invasionQ30008770
Fascin actin bundling controls podosome turnover and disassembly while cortactin is involved in podosome assembly by its SH3 domain in THP-1 macrophages and dendritic cellsQ30009246
Stratifying fascin and cortactin function in invadopodium formation using inhibitory nanobodies and targeted subcellular delocalizationQ30009497
Hydrogen-deuterium exchange mass spectrometry for determining protein structural changes in drug discovery.Q30372448
Live imaging of endogenous protein dynamics in zebrafish using chromobodiesQ30651427
Real-time analysis of epithelial-mesenchymal transition using fluorescent single-domain antibodiesQ30661925
Fascin Rigidity and L-plastin Flexibility Cooperate in Cancer Cell Invadopodia and FilopodiaQ30755124
Single domain camel antibodies: current status.Q31005004
Breaking the diffraction barrier: super-resolution imaging of cellsQ33774961
Direct injection of functional single-domain antibodies from E. coli into human cellsQ33775617
A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cellsQ34260840
A LOV2 domain-based optogenetic tool to control protein degradation and cellular function.Q34340388
Cell penetrable humanized-VH/V(H)H that inhibit RNA dependent RNA polymerase (NS5B) of HCV.Q34474314
Molecular basis for the preferential cleft recognition by dromedary heavy-chain antibodiesQ34574853
Investigating the interaction between the neonatal Fc receptor and monoclonal antibody variants by hydrogen/deuterium exchange mass spectrometryQ34884133
Functional inhibition of β-catenin-mediated Wnt signaling by intracellular VHH antibodiesQ35530296
The peroxisomal protein import machinery displays a preference for monomeric substrates.Q35575737
Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish.Q35806361
Saccharomyces cerevisiae peroxisomal thiolase is imported as a dimer.Q35861320
Peptides in headlock--a novel high-affinity and versatile peptide-binding nanobody for proteomics and microscopyQ35899535
Nanobody-targeted E3-ubiquitin ligase complex degrades nuclear proteinsQ36064452
Proteomics of organelles and large cellular structuresQ36289104
Inducible, reversible system for the rapid and complete degradation of proteins in mammalian cellsQ36471246
Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation.Q36585908
A strategy for dissecting the architectures of native macromolecular assembliesQ36716406
Resolving bundled microtubules using anti-tubulin nanobodies.Q37031835
Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodiesQ37042620
A Camelid-derived Antibody Fragment Targeting the Active Site of a Serine Protease Balances between Inhibitor and Substrate BehaviorQ37098934
Chromatibody, a novel non-invasive molecular tool to explore and manipulate chromatin in living cellsQ37120393
Allosteric nanobodies reveal the dynamic range and diverse mechanisms of G-protein-coupled receptor activationQ37127495
A new survivin tracer tracks, delocalizes and captures endogenous survivin at different subcellular locations and in distinct organellesQ37169099
Stepwise engineering of heterodimeric single domain camelid VHH antibodies that passively protect mice from ricin toxin.Q37405037
Regulation of β2-adrenergic receptor function by conformationally selective single-domain intrabodies.Q37603933
Recognizing and avoiding siRNA off-target effects for target identification and therapeutic applicationQ37665303
A nanobody targeting the F-actin capping protein CapG restrains breast cancer metastasisQ37690960
Precisely and accurately localizing single emitters in fluorescence microscopyQ38191782
Characterizing Protein-Protein Interactions Using Mass Spectrometry: Challenges and OpportunitiesQ38782296
Nanobodies as therapeutics: big opportunities for small antibodiesQ38808711
A nanobody modulates the p53 transcriptional program without perturbing its functional architectureQ38946984
Nuclear pore scaffold structure analyzed by super-resolution microscopy and particle averaging.Q39127202
Mapping cytoskeletal protein function in cells by means of nanobodies.Q39131836
Sortase A-mediated site-specific labeling of camelid single-domain antibody-fragments: a versatile strategy for multiple molecular imaging modalities.Q39793253
An alpaca single-domain antibody blocks filopodia formation by obstructing L-plastin-mediated F-actin bundlingQ39804301
Backbone NMR reveals allosteric signal transduction networks in the β1-adrenergic receptorQ40028443
Sortagging: a versatile method for protein labelingQ40077031
Structural Analysis of Single Domain Antibodies Bound to a Second Neutralizing Hot Spot on Ricin Toxin's Enzymatic SubunitQ40428974
Chaperone nanobodies protect gelsolin against MT1-MMP degradation and alleviate amyloid burden in the gelsolin amyloidosis mouse modelQ40569183
Structural analysis of nested neutralizing and non-neutralizing B cell epitopes on ricin toxin's enzymatic subunit.Q40682149
Trapping mammalian protein complexes in viral particles.Q40778539
An oligomeric protein is imported into peroxisomes in vivoQ41418161
P407language of work or nameEnglishQ1860
P921main subjectprotein-protein interactionQ896177
P304page(s)771
P577publication date2017-07-04
P1433published inFrontiers in ImmunologyQ27723748
P1476titleNanobody Technology: A Versatile Toolkit for Microscopic Imaging, Protein-Protein Interaction Analysis, and Protein Function Exploration
P478volume8

Reverse relations

cites work (P2860)
Q48509449A cDNA Immunization Strategy to Generate Nanobodies against Membrane Proteins in Native Conformation.
Q64078400A nanobody-based molecular toolkit provides new mechanistic insight into clathrin-coat initiation
Q48256320A small protein probe for correlated microscopy of endogenous proteins.
Q83227810A toolbox of nanobodies developed and validated for use as intrabodies and nanoscale immunolabels in mammalian brain neurons
Q90753227BRAIN Initiative: Cutting-Edge Tools and Resources for the Community
Q50116259Bridging the gap: Super-resolution microscopy of epithelial cell junctions
Q47135878Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook
Q59807398Cellular Complexity in MAPK Signaling in Plants: Questions and Emerging Tools to Answer Them
Q57799929Cortactin and fascin-1 regulate extracellular vesicle release by controlling endosomal trafficking or invadopodia formation and function
Q90211754Current Approaches and Future Perspectives for Nanobodies in Stroke Diagnostic and Therapy
Q57153658DARPins recognizing mTFP1 as novel reagents for and protein manipulations
Q52576113Development by Genetic Immunization of Monovalent Antibodies Against Human Vasoactive Intestinal Peptide Receptor 1 (VPAC1), New Innovative, and Versatile Tools to Study VPAC1 Receptor Function
Q91912743Domain swapping of complementarity-determining region in nanobodies produced by Pichia pastoris
Q99636619Exploring cellular biochemistry with nanobodies
Q64228410From Desert to Medicine: A Review of Camel Genomics and Therapeutic Products
Q64112048Generation, expression and utilization of single-domain antibodies for in vivo protein localization and manipulation in sea urchin embryos
Q90494776Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks
Q91557881High-dimensional cell-level analysis of tissues with Ce3D multiplex volume imaging
Q55021480Identification of Functional mimotopes of human Vasorin Ectodomain by Biopanning.
Q57175582Intranucleus Single-Molecule Imaging in Living Cells
Q90212392Isolation and Characterization of Nanobodies against a Zinc-Transporting P-Type ATPase
Q90298981Molecularly Engineered Nanobodies for Tunable Pharmacokinetics and Drug Delivery
Q47099672Nanobodies and Nanobody-Based Human Heavy Chain Antibodies As Antitumor Therapeutics.
Q55025877Nanobody-Based Biologics for Modulating Purinergic Signaling in Inflammation and Immunity.
Q96647703Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium
Q93086703Nb-induced stabilisation of p53 in HPV-infected cells
Q47975495Organellar Omics-A Reviving Strategy to Untangle the Biomolecular Complexity of the Cell
Q91843130Preparation and characterization of a single-domain antibody specific for the porcine epidemic diarrhea virus spike protein
Q50026240Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical reagents.
Q91610417Single-Domain Antibodies Represent Novel Alternatives to Monoclonal Antibodies as Targeting Agents against the Human Papillomavirus 16 E6 Protein
Q56556960Single-domain antibodies as promising experimental tools in imaging and isolation of porcine epidemic diarrhea virus
Q90999189Structural and thermodynamic basis for the recognition of the substrate-binding cleft on hen egg lysozyme by a single-domain antibody
Q90211845Using Nanobodies to Study Protein Function in Developing Organisms
Q91801116Visualization of Bacterial Protein Complexes Labeled with Fluorescent Proteins and Nanobody Binders for STED Microscopy

Search more.