review article | Q7318358 |
scholarly article | Q13442814 |
P819 | ADS bibcode | 1987Sci...236.1252K |
P356 | DOI | 10.1126/SCIENCE.3296192 |
P8608 | Fatcat ID | release_rq3sbdasezgzpgks6k57ajcue4 |
P698 | PubMed publication ID | 3296192 |
P2093 | author name string | J R Knowles | |
P433 | issue | 4806 | |
P407 | language of work or name | English | Q1860 |
P304 | page(s) | 1252-1258 | |
P577 | publication date | 1987-06-01 | |
P1433 | published in | Science | Q192864 |
P1476 | title | Tinkering with enzymes: what are we learning? | |
P478 | volume | 236 |
Q34990932 | A highly conserved arginine residue of the chitosanase from Streptomyces sp. N174 is involved both in catalysis and substrate binding |
Q42004261 | A polyclonal antibody preparation with Michaelian catalytic properties. |
Q30326383 | Active-site variants of Streptomyces griseus protease B with peptide-ligation activity. |
Q27735251 | Additivity of protein-guanine interactions in ribonuclease T1 |
Q41817502 | Albino mutants of Streptomyces glaucescens tyrosinase |
Q35769704 | Amino acid misincorporation during high-level expression of mouse epidermal growth factor inEscherichia coli |
Q35527954 | Applied molecular evolution |
Q39548353 | Biocatalysis made to order |
Q56899203 | Biological chemistry: catalytic competition for cells |
Q46135712 | Calculations of free energy profiles for the staphylococcal nuclease catalyzed reaction |
Q42827159 | Catalytic activity of bovine seminal ribonuclease is essential for its immunosuppressive and other biological activities |
Q35511384 | Catalytic sites of Escherichia coli F1-ATPase |
Q38302768 | Changing the substrate specificity of penicillin G acylase from Kluyvera citrophila through selective pressure |
Q41865481 | Characterization of alanine to valine sequence variants in the Fc region of nivolumab biosimilar produced in Chinese hamster ovary cells |
Q72167570 | Cleavage of factor VIII light chain is required for maximal generation of factor VIIIa activity |
Q72325765 | Comparative studies on S-adenosyl-L-methionine binding sites of protein N-methyltransferases, using 8-azido-S-adenosyl-L-methionine as photoaffinity probe |
Q39559168 | Complementary use of chemical modification and site-directed mutagenesis to probe structure-activity relationships in enzymes |
Q40488310 | Conformational instability of human prion protein upon residue modification: a molecular dynamics simulation study |
Q35213319 | Conformational stability and catalytic activity of PTEN variants linked to cancers and autism spectrum disorders |
Q35112213 | Conservation of the biologically active portions of staphylococcal enterotoxins C1 and C2 |
Q35900161 | Critical hydrogen bonding by serine 235 for cephalosporinase activity of TEM-1 beta-lactamase |
Q51647652 | Crystal structures of peptidic catalysts of the H-dPro-Pro-Xaa type. |
Q42161838 | Cysteine-86 is not needed for the enzymic activity of glutathione S-transferase 3-3 |
Q41907646 | Cytotoxicity of RNase Sa to the acute myeloid leukemia Kasumi-1 cells depends on the net charge |
Q27658994 | Dissecting the paradoxical effects of hydrogen bond mutations in the ketosteroid isomerase oxyanion hole |
Q41107396 | Dissection of functional domains of the yeast proton-pumping ATPase by directed mutagenesis. |
Q31065498 | Diverse interactions between the individual mutations in a double mutant at the active site of staphylococcal nuclease |
Q30333704 | Effects of tryptophan mutation on the deprotonation and reprotonation kinetics of the Schiff base during the photocycle of bacteriorhodopsin |
Q68844981 | Energy minimization and molecular dynamics studies of Asn-102 elastase |
Q34336902 | Enzyme catalysis: removing chemically 'essential' residues by site-directed mutagenesis. |
Q64106148 | Esterification Delivers a Functional Enzyme into a Human Cell |
Q42120197 | Evaluation of catalytic free energies in genetically modified proteins |
Q41963314 | Evidence from a mutant beta-lactamase for the mechanism of beta-lactamase-catalysed depsipeptide aminolysis |
Q24651553 | Evidence that glutamic acid 167 is an active-site residue of Shiga-like toxin I |
Q38259642 | Expression and mutation of Ca2+ ATPases of the sarcoplasmic reticulum |
Q34372184 | Functional analysis of the Shiga toxin and Shiga-like toxin type II variant binding subunits by using site-directed mutagenesis. |
Q35555850 | Functionally-stabilized proteins — A review |
Q42177492 | Glycine to glutamic acid misincorporation observed in a recombinant protein expressed by Escherichia coli cells |
Q37698471 | High-efficiency oligonucleotide-directed plasmid mutagenesis |
Q42025489 | Hydrogen-bonding in enzyme catalysis. Fourier-transform infrared detection of ground-state electronic strain in acyl-chymotrypsins and analysis of the kinetic consequences |
Q33852277 | Identification of amino acid residues essential for the enzymatic activities of pertussis toxin |
Q28357343 | Identification of signalling and non-signalling binding contributions to enzyme reactivity. Alternative combinations of binding interactions provide for change in transition-state geometry in reactions of papain |
Q36129775 | Identification of three amino acid residues in the B subunit of Shiga toxin and Shiga-like toxin type II that are essential for holotoxin activity |
Q35222975 | Internal water molecules and H‐bonding in biological macromolecules: A review of structural features with functional implications |
Q67726902 | Investigation of the mechanisms of irreversible thermoinactivation of Bacillus stearothermophilus alpha-amylase |
Q36394814 | Is more better? A comparison of tri- and tetrapeptidic catalysts. |
Q44754423 | Learning from directed evolution: theoretical investigations into cooperative mutations in lipase enantioselectivity |
Q33178906 | Mass spectrometric identification of a stable catalytic cysteinesulfinic acid residue in an enzymatically active chemically modified glucoamylase mutant |
Q57013883 | Methodological aspects and potential of computer‐aided protein engineering |
Q37143315 | Molecular Genetics and Drug Discovery |
Q40667232 | Multi-stage proofreading in DNA replication |
Q39606736 | Multiplicity of beta-1,4-xylanase in microorganisms: functions and applications |
Q37372924 | Mutation of the active site carboxy-lysine (K70) of OXA-1 beta-lactamase results in a deacylation-deficient enzyme |
Q36165342 | Mutational analysis of the Shiga toxin and Shiga-like toxin II enzymatic subunits |
Q35800059 | Mutational studies of protein structures and their stabilities |
Q39838747 | Onconase cytotoxicity relies on the distribution of its positive charge |
Q35285491 | Organocatalysts of oxidative protein folding inspired by protein disulfide isomerase |
Q28610143 | Ornithine delta-aminotransferase mutations in gyrate atrophy. Allelic heterogeneity and functional consequences |
Q31160273 | Permissible amino acid substitutions within the putative nucleoside binding site of herpes simplex virus type 1 encoded thymidine kinase established by random sequence mutagenesis [corrected] |
Q46110843 | Phage display of proteases and macromolecular inhibitors |
Q43057995 | Polyarginine as a multifunctional fusion tag. |
Q41834608 | Probing the origin of the compromised catalysis of E. coli alkaline phosphatase in its promiscuous sulfatase reaction |
Q35014053 | Protein engineering 20 years on. |
Q39677448 | Protein engineering. The design, synthesis and characterization of factitious proteins |
Q41835930 | Protein prosthesis: 1,5-disubstituted[1,2,3]triazoles as cis-peptide bond surrogates |
Q41220419 | Protein prosthesis: β-peptides as reverse-turn surrogates |
Q35767603 | Rational design of nascent metalloenzymes |
Q30821032 | Reactivity of intein thioesters: appending a functional group to a protein |
Q33267646 | Receptor and antibody interactions of human interleukin-3 characterized by mutational analysis |
Q41240571 | Recombinant human protein C derivatives: altered response to calcium resulting in enhanced activation by thrombin |
Q41506231 | Replacing a single atom accelerates the folding of a protein and increases its thermostability |
Q24559878 | Searching sequence space by definably random mutagenesis: improving the catalytic potency of an enzyme |
Q39921731 | Selection of amidases with novel substrate specificities from penicillin amidase of Escherichia coli |
Q52451866 | Simulation analysis of the stability mutant R96H of T4 lysozyme. |
Q38297775 | Single amino acids changes in the signal receptor domain of XylR resulted in mutants that stimulate transcription in the absence of effectors. |
Q28318679 | Site-directed mutagenesis and chemical modification of cysteine residues of rat glutathione S-transferase 3-3 |
Q38334994 | Site-directed mutagenesis of dicarboxylic acids near the active site of Bacillus cereus 5/B/6 beta-lactamase II. |
Q33828274 | Site-directed mutagenesis of the catalytic residues Asp-52 and Glu-35 of chicken egg white lysozyme |
Q41647221 | Structural studies of natural product biosynthetic proteins |
Q38238531 | Study of contractile and cytoskeletal proteins using Drosophila genetics |
Q24557444 | Substrate specificity of trypsin investigated by using a genetic selection |
Q28361241 | Supracrystallographic resolution of interactions contributing to enzyme catalysis by use of natural structural variants and reactivity-probe kinetics |
Q55427345 | Synthetic Biology, Tinkering Biology, and Artificial Biology. What are We Learning? |
Q33988336 | Synthetic biology |
Q36223085 | T7 RNA polymerase mutants with altered promoter specificities |
Q36053941 | The design of synthetic genes |
Q36511734 | The energetics of intramolecular reactions and enzyme catalysis |
Q36734675 | The in vitro biological effect of nerve growth factor is inhibited by synthetic peptides |
Q36276424 | Thymidine kinase mutants obtained by random sequence selection |
Q30682990 | Uncovering the determinants of a highly perturbed tyrosine pKa in the active site of ketosteroid isomerase |
Q33580946 | Use of site-directed mutagenesis to elucidate the role of arginine-166 in the catalytic mechanism of alkaline phosphatase |
Q42792741 | Variation in the P2-S2 stereochemical selectivity towards the enantiomeric N-acetylphenylalanylglycine 4-nitroanilides among the cysteine proteinases papain, ficin and actinidin |
Q36632309 | Varicella-zoster virus open reading frame 4 encodes a transcriptional activator that is functionally distinct from that of herpes simplex virus homology ICP27. |
Q57840172 | Wege zu neuen Enzymen: Protein Engineering und Katalytische Antikörper |
Q50781033 | X-Neu5Ac: a novel substrate for chromogenic assay of neuraminidase activity in bacterial expression systems. |
Q38348221 | beta-lactamase I from Bacillus cereus. Structure and site-directed mutagenesis |
Search more.