Development of bimolecular fluorescence complementation using Dronpa for visualization of protein-protein interactions in cells

scientific article published on October 2010

Development of bimolecular fluorescence complementation using Dronpa for visualization of protein-protein interactions in cells is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.1007/S11307-010-0312-2
P698PubMed publication ID20373040

P50authorIck Chan KwonQ67123705
P2093author name stringKi-Hyun Nam
Kwang Yeon Hwang
Lin-Woo Kang
Ji Hyung Chung
Ye Sun Han
Jong-Hwa Park
Soo-Hyun Hahm
You Ri Lee
P2860cites workFluorescence-based methods in the study of protein-protein interactions in living cellsQ37207440
The human Rad9-Rad1-Hus1 checkpoint complex stimulates flap endonuclease 1.Q37695856
Physical and functional interactions between MutY glycosylase homologue (MYH) and checkpoint proteins Rad9-Rad1-Hus1.Q39086313
Visualizing neurons one-by-one in vivo: optical dissection and reconstruction of neural networks with reversible fluorescent proteinsQ40335095
Disruption of the Rad9/Rad1/Hus1 (9-1-1) complex leads to checkpoint signaling and replication defectsQ40547899
Human hRad1 but not hRad9 protects hHus1 from ubiquitin-proteasomal degradationQ40560300
Monitoring Protein−Protein Interactions Using Split Synthetic Renilla Luciferase Protein-Fragment-Assisted ComplementationQ40653800
Complementary methods for studies of protein interactions in living cellsQ42380047
Subcellular dynamics of protein kinase A activity visualized by FRET-based reportersQ42499376
Interaction of checkpoint proteins Hus1/Rad1/Rad9 with DNA base excision repair enzyme MutY homolog in fission yeast, Schizosaccharomyces pombeQ45144500
The Rad9-Hus1-Rad1 checkpoint clamp regulates interaction of TopBP1 with ATR.Q45936344
An improved mRFP1 adds red to bimolecular fluorescence complementation.Q50477387
Simultaneous visualization of two protein complexes in a single plant cell using multicolor fluorescence complementation analysis.Q50602744
Use of bimolecular fluorescence complementation in yeast Saccharomyces cerevisiae.Q50610007
The two DNA clamps Rad9/Rad1/Hus1 complex and proliferating cell nuclear antigen differentially regulate flap endonuclease 1 activity.Q53654201
8-hydroxyguanine (7,8-dihydro-8-oxoguanine) DNA glycosylase and AP lyase activities of hOGG1 protein and their substrate specificityQ73886290
Human MutY homolog, a DNA glycosylase involved in base excision repair, physically and functionally interacts with mismatch repair proteins human MutS homolog 2/human MutS homolog 6Q24292187
The human checkpoint sensor and alternative DNA clamp Rad9-Rad1-Hus1 modulates the activity of DNA ligase I, a component of the long-patch base excision repair machineryQ24301431
Crystal structure of the human rad9-hus1-rad1 clampQ24313157
Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesisQ24318724
Loading of the human 9-1-1 checkpoint complex onto DNA by the checkpoint clamp loader hRad17-replication factor C complex in vitroQ24550745
Kinetics of regulated protein-protein interactions revealed with firefly luciferase complementation imaging in cells and living animalsQ24563431
Regulation of ATR substrate selection by Rad17-dependent loading of Rad9 complexes onto chromatinQ24672721
Crystallography & NMR System: A New Software Suite for Macromolecular Structure DeterminationQ26778405
Structural characterization of the photoswitchable fluorescent protein Dronpa-C62SQ27643734
Light-dependent regulation of structural flexibility in a photochromic fluorescent proteinQ27650928
Crystal structure of the rad9-rad1-hus1 DNA damage checkpoint complex--implications for clamp loading and regulationQ27655485
Cloning and sequencing a human homolog (hMYH) of the Escherichia coli mutY gene whose function is required for the repair of oxidative DNA damageQ28114912
A monomeric red fluorescent proteinQ28131782
Human homolog of the MutY repair protein (hMYH) physically interacts with proteins involved in long patch DNA base excision repairQ28138546
Identification of human MutY homolog (hMYH) as a repair enzyme for 2-hydroxyadenine in DNA and detection of multiple forms of hMYH located in nuclei and mitochondriaQ28610376
The fluorescent toolbox for assessing protein location and functionQ29615075
Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementationQ29616037
Clamp and clamp loader structures of the human checkpoint protein complexes, Rad9-1-1 and Rad17-RFC.Q32184097
Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cellsQ33280958
Structure-based predictions of Rad1, Rad9, Hus1 and Rad17 participation in sliding clamp and clamp-loading complexesQ33617643
Fluorescent proteins as a toolkit for in vivo imagingQ33991259
Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlightingQ34369228
Phosphorylation of Xenopus Rad1 and Hus1 defines a readout for ATR activation that is independent of Claspin and the Rad9 carboxy terminusQ34483565
Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysisQ34648424
Protein fragment complementation strategies for biochemical network mappingQ35603040
The human Rad9/Rad1/Hus1 damage sensor clamp interacts with DNA polymerase beta and increases its DNA substrate utilisation efficiency: implications for DNA repairQ35618148
The checkpoint clamp, Rad9-Rad1-Hus1 complex, preferentially stimulates the activity of apurinic/apyrimidinic endonuclease 1 and DNA polymerase beta in long patch base excision repairQ35829262
The human checkpoint sensor Rad9-Rad1-Hus1 interacts with and stimulates NEIL1 glycosylaseQ35829284
The human checkpoint sensor Rad9-Rad1-Hus1 interacts with and stimulates DNA repair enzyme TDG glycosylaseQ36168692
Innovation: Photoactivatable fluorescent proteins.Q36259091
Bimolecular fluorescence complementation (BiFC) analysis of protein interactions in Caenorhabditis elegansQ36943412
P433issue5
P921main subjectvisualizationQ451553
protein-protein interactionQ896177
P304page(s)468-478
P577publication date2010-10-01
P1433published inMolecular Imaging and BiologyQ6895951
P1476titleDevelopment of bimolecular fluorescence complementation using Dronpa for visualization of protein-protein interactions in cells
P478volume12

Reverse relations

cites work (P2860)
Q37133219A near-infrared BiFC reporter for in vivo imaging of protein-protein interactions
Q82523832An Improved Bimolecular Fluorescence Complementation Assay with a High Signal-To-Noise Ratio
Q35562951Bimolecular Fluorescence Complementation (BiFC) Analysis: Advances and Recent Applications for Genome-Wide Interaction Studies
Q33824525Development of bimolecular fluorescence complementation using rsEGFP2 for detection and super-resolution imaging of protein-protein interactions in live cells
Q34280594Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system
Q27322523Photoactivated localization microscopy with bimolecular fluorescence complementation (BiFC-PALM) for nanoscale imaging of protein-protein interactions in cells
Q33816050Protein fragment bimolecular fluorescence complementation analyses for the in vivo study of protein-protein interactions and cellular protein complex localizations.
Q38805265RefSOFI for Mapping Nanoscale Organization of Protein-Protein Interactions in Living Cells.
Q64288780Split Green Fluorescent Proteins: Scope, Limitations, and Outlook
Q30584809Super-resolution imaging and tracking of protein-protein interactions in sub-diffraction cellular space
Q50046568Surveying the landscape of optogenetic methods for detection of protein-protein interactions.
Q30498214Visualization of protein interactions in living Drosophila embryos by the bimolecular fluorescence complementation assay
Q34116957Visualization of the physical and functional interaction between hMYH and hRad9 by Dronpa bimolecular fluorescence complementation

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