Leucines 193 and 194 at the N-terminal domain of the XylS protein, the positive transcriptional regulator of the TOL meta-cleavage pathway, are involved in dimerization

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Leucines 193 and 194 at the N-terminal domain of the XylS protein, the positive transcriptional regulator of the TOL meta-cleavage pathway, are involved in dimerization is …
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scholarly articleQ13442814

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P356DOI10.1128/JB.185.10.3036-3041.2003
P932PMC publication ID154087
P698PubMed publication ID12730162

P50authorJuan Luis Ramos MartínQ21168777
Silvia MarquésQ42291217
P2093author name stringRaquel Ruíz
P2860cites workFunctional domains of the AraC proteinQ36367901
Altered effector specificities in regulators of gene expression: TOL plasmid xylS mutants and their use to engineer expansion of the range of aromatics degraded by bacteriaQ37406839
XylS domain interactions can be deduced from intraallelic dominance in double mutants of Pseudomonas putidaQ38324734
Novel group of virulence activators within the AraC family that are not restricted to upstream binding sitesQ39517760
AraC-XylS database: a family of positive transcriptional regulators in bacteriaQ39524366
DNA binding of the transcription activator protein MelR from Escherichia coli and its C-terminal domainQ39615959
Expression of the TOL plasmid xylS gene in Pseudomonas putida occurs from a alpha 70-dependent promoter or from alpha 70- and alpha 54-dependent tandem promoters according to the compound used for growthQ39841051
Transposon mutagenesis analysis of meta-cleavage pathway operon genes of the TOL plasmid of Pseudomonas putida mt-2Q39965774
Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulatorsQ41620651
Overexpression, purification and characterization of the Escherichia coli MelR transcription activator proteinQ41761485
Residues 137 and 153 at the N terminus of the XylS protein influence the effector profile of this transcriptional regulator and the sigma factor used by RNA polymerase to stimulate transcription from its cognate promoterQ43829905
XylS activator and RNA polymerase binding sites at the Pm promoter overlapQ44002052
Identification of critical amino-terminal regions of XylS. The positive regulator encoded by the TOL plasmidQ54257962
A general system to integrate lacZ fusions into the chromosomes of gram-negative eubacteria: regulation of the Pm promoter of the TOL plasmid studied with all controlling elements in monocopy.Q54271765
Design of a solubilization pathway for recombinant polypeptides in vivo through processing of a bi-protein with a viral protease.Q54564518
Chromosomal insertion of the entire Escherichia coli lactose operon, into two strains of Pseudomonas, using a modified mini-Tn5 delivery system.Q54570429
Mutational analysis of the highly conserved C-terminal residues of the XylS protein, a member of the AraC family of transcriptional regulatorsQ57340422
Critical Nucleotides in the Upstream Region of the XylS-dependent TOLmeta-Cleavage Pathway Operon Promoter as Deduced from Analysis of MutantsQ57340435
The XylS-dependent Pm promoter is transcribed in vivo by RNA polymerase with sigma32 or sigma38 depending on the growth phaseQ57340438
Signal-regulator interactions, genetic analysis of the effector binding site of xyls, the benzoate-activated positive regulator of Pseudomonas TOL plasmid meta-cleavage pathway operonQ57340481
A genetic system to study the in vivo role of transcriptional regulators in Escherichia coliQ68203618
The C-terminal end of AraC tightly binds to the rest of its domainQ73265509
Crystal structure of the Escherichia coli Rob transcription factor in complex with DNAQ27622490
Crystallographic evidence of a large ligand-induced hinge-twist motion between the two domains of the maltodextrin binding protein involved in active transport and chemotaxisQ27642154
Structural basis for ligand-regulated oligomerization of AraCQ27735817
A novel DNA-binding motif in MarA: the first structure for an AraC family transcriptional activatorQ27765261
Site-directed mutagenesis by overlap extension using the polymerase chain reactionQ27860503
Studies on transformation of Escherichia coli with plasmidsQ27860598
Identification of a central regulator of stationary-phase gene expression inEscherichia coliQ27976519
Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fusedQ28142404
The 2.3-A resolution structure of the maltose- or maltodextrin-binding protein, a primary receptor of bacterial active transport and chemotaxisQ28268122
The gdhB gene of Pseudomonas aeruginosa encodes an arginine-inducible NAD(+)-dependent glutamate dehydrogenase which is subject to allosteric regulationQ28492804
Arac/XylS family of transcriptional regulatorsQ28776772
Characterization of the oligomeric states of wild type and mutant AraC.Q30168745
Strengthened arm-dimerization domain interactions in AraC.Q31538428
Interactions of the XylS regulators with the C-terminal domain of the RNA polymerase alpha subunit influence the expression level from the cognate Pm promoterQ32148321
Catabolite gene activator protein mutations affecting activity of the araBAD promoter.Q33722326
Regulation of the L-arabinose operon of Escherichia coliQ33927032
Identification of the domains of UreR, an AraC-like transcriptional regulator of the urease gene cluster in Proteus mirabilisQ33996633
The AraC transcriptional activatorsQ34204972
Collaborative regulation of Escherichia coli glutamate-dependent acid resistance by two AraC-like regulators, GadX and GadW (YhiW)Q34321388
Growing repertoire of AraC/XylS activatorsQ34436978
P433issue10
P407language of work or nameEnglishQ1860
P304page(s)3036-3041
P577publication date2003-05-01
P1433published inJournal of BacteriologyQ478419
P1476titleLeucines 193 and 194 at the N-terminal domain of the XylS protein, the positive transcriptional regulator of the TOL meta-cleavage pathway, are involved in dimerization
P478volume185

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cites work (P2860)
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Q30155885Directed evolution of the transcription factor XylS for development of improved expression systems
Q37843663Exploitation of prokaryotic expression systems based on the salicylate-dependent control circuit encompassing nahR/P(sal)::xylS2 for biotechnological applications.
Q35710852Functional Characterization of the Mannitol Promoter of Pseudomonas fluorescens DSM 50106 and Its Application for a Mannitol-Inducible Expression System for Pseudomonas putida KT2440
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Q51122851Mycobacterium tuberculosis Rv1474c is a TetR-like transcriptional repressor that regulates aconitase, an essential enzyme and RNA-binding protein, in an iron-responsive manner.
Q92320401Physical decoupling of XylS/Pm regulatory elements and conditional proteolysis enable precise control of gene expression in Pseudomonas putida
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Q42991149Regulation of the expression level of transcription factor XylS reveals new functional insight into its induction mechanism at the Pm promoter
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Q41893759Roles of effectors in XylS-dependent transcription activation: intramolecular domain derepression and DNA binding
Q34297881Self-association is required for occupation of adjacent binding sites in Pseudomonas aeruginosa type III secretion system promoters.
Q40752789Sequential XylS-CTD binding to the Pm promoter induces DNA bending prior to activation
Q34650292The TodS-TodT two-component regulatory system recognizes a wide range of effectors and works with DNA-bending proteins
Q39170128The XylS/Pm regulator/promoter system and its use in fundamental studies of bacterial gene expression, recombinant protein production and metabolic engineering
Q36483692The chaperone IpgC copurifies with the virulence regulator MxiE

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