Generation of eGFP expressing recombinant Zaire ebolavirus for analysis of early pathogenesis events and high-throughput antiviral drug screening

scientific article published on February 2005

Generation of eGFP expressing recombinant Zaire ebolavirus for analysis of early pathogenesis events and high-throughput antiviral drug screening is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1016/J.VIROL.2004.10.048
P698PubMed publication ID15661137
P5875ResearchGate publication ID8070592

P50authorStuart T. NicholQ60415973
Jonathan S TownerQ87983083
John W. HugginsQ113070223
P2093author name stringCynthia S Goldsmith
Jason Paragas
Manisha Gupta
Jason E Dover
P2860cites workObservation of measles virus cell-to-cell spread in astrocytoma cells by using a green fluorescent protein-expressing recombinant virus.Q33823630
3-deazaneplanocin A induces massively increased interferon-alpha production in Ebola virus-infected miceQ33959778
Treatment of Ebola virus infection with a recombinant inhibitor of factor VIIa/tissue factor: a study in rhesus monkeysQ33974706
Antiviral drug therapy of filovirus infections: S-adenosylhomocysteine hydrolase inhibitors inhibit Ebola virus in vitro and in a lethal mouse modelQ34068520
Mannosyl glycodendritic structure inhibits DC-SIGN-mediated Ebola virus infection in cis and in transQ34230933
Reverse genetics demonstrates that proteolytic processing of the Ebola virus glycoprotein is not essential for replication in cell cultureQ34326549
Rapid diagnosis of Ebola hemorrhagic fever by reverse transcription-PCR in an outbreak setting and assessment of patient viral load as a predictor of outcomeQ37093682
Cyanovirin-N binds to the viral surface glycoprotein, GP1,2 and inhibits infectivity of Ebola virusQ38355197
Infection and activation of monocytes by Marburg and Ebola virusesQ39605104
A simple assay for determining antiviral activity against Crimean-Congo hemorrhagic fever virusQ40538104
Protection from lethal infection is determined by innate immune responses in a mouse model of Ebola virus infectionQ40552244
Monocyte-derived human macrophages and peripheral blood mononuclear cells infected with ebola virus secrete MIP-1alpha and TNF-alpha and inhibit poly-IC-induced IFN-alpha in vitro.Q40602946
Ebola virus defective interfering particles and persistent infectionQ40632676
Pathogenesis of experimental Ebola virus infection in guinea pigsQ44089764
A mouse model for evaluation of prophylaxis and therapy of Ebola hemorrhagic feverQ44089779
Elucidation of Nipah virus morphogenesis and replication using ultrastructural and molecular approachesQ45728992
P433issue1
P407language of work or nameEnglishQ1860
P921main subjectZaire ebolavirusQ8064876
drug screeningQ10505555
urban ecologyQ1430301
P1104number of pages8
P304page(s)20-27
P577publication date2005-02-01
P1433published inVirologyQ7934867
P1476titleGeneration of eGFP expressing recombinant Zaire ebolavirus for analysis of early pathogenesis events and high-throughput antiviral drug screening
P478volume332

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cites work (P2860)
Q40070709A Sensitive in Vitro High-Throughput Screen To Identify Pan-filoviral Replication Inhibitors Targeting the VP35-NP Interface.
Q34665879A chemotype that inhibits three unrelated pathogenic targets: the botulinum neurotoxin serotype A light chain, P. falciparum malaria, and the Ebola filovirus.
Q34999859A fusion-inhibiting peptide against Rift Valley fever virus inhibits multiple, diverse viruses
Q37209733A novel Ebola virus expressing luciferase allows for rapid and quantitative testing of antivirals
Q34042685A novel L-ficolin/mannose-binding lectin chimeric molecule with enhanced activity against Ebola virus.
Q30588041A novel porcine reproductive and respiratory syndrome virus vector system that stably expresses enhanced green fluorescent protein as a separate transcription unit
Q43106551A recombinant, infectious human parainfluenza virus type 3 expressing the enhanced green fluorescent protein for use in high-throughput antiviral assays
Q46247452A review on the antagonist Ebola: A prophylactic approach.
Q30279239A screen of approved drugs and molecular probes identifies therapeutics with anti-Ebola virus activity
Q36040533Aerosolized Ebola vaccine protects primates and elicits lung-resident T cell responses
Q37252982An Ebola Virus-Like Particle-Based Reporter System Enables Evaluation of Antiviral Drugs In Vivo under Non-Biosafety Level 4 Conditions
Q60193415An emergent Ebola virus nucleoprotein variant influences virion budding, oligomerization, transcription and replication
Q89548894Analysis of a Therapeutic Antibody Cocktail Reveals Determinants for Cooperative and Broad Ebolavirus Neutralization
Q58779740Antibody Repertoires to the Same Ebola Vaccine Antigen Are Differentially Affected by Vaccine Vectors
Q37728345Antibody-dependent-cellular-cytotoxicity-inducing antibodies significantly affect the post-exposure treatment of Ebola virus infection
Q33538454Antiviral activity of a small-molecule inhibitor of filovirus infection
Q26750838Applications of Replicating-Competent Reporter-Expressing Viruses in Diagnostic and Molecular Virology
Q54218743Broadly neutralizing antibodies from human survivors target a conserved site in the Ebola virus glycoprotein HR2-MPER region.
Q37190830Chemical modifications of antisense morpholino oligomers enhance their efficacy against Ebola virus infection
Q36736403Chimeric Filoviruses for Identification and Characterization of Monoclonal Antibodies
Q40295821Conserved receptor-binding domains of Lake Victoria marburgvirus and Zaire ebolavirus bind a common receptor
Q40243605Crimean-Congo hemorrhagic fever virus utilizes a clathrin- and early endosome-dependent entry pathway
Q36522002Cross-Reactive and Potent Neutralizing Antibody Responses in Human Survivors of Natural Ebolavirus Infection
Q64106080Cross-reactive neutralizing human survivor monoclonal antibody BDBV223 targets the ebolavirus stalk
Q37690397Development and application of reporter-expressing mononegaviruses: current challenges and perspectives
Q40149516Development of a reverse genetics system to generate a recombinant Ebola virus Makona expressing a green fluorescent protein
Q42266175Development of a reverse genetics system to generate recombinant Marburg virus derived from a bat isolate
Q40353545Development of high-content imaging assays for lethal viral pathogens.
Q36379341Development of treatment strategies to combat Ebola and Marburg viruses
Q40105589Discovery of a Broad-Spectrum Antiviral Compound That Inhibits Pyrimidine Biosynthesis and Establishes a Type 1 Interferon-Independent Antiviral State
Q57028377Disruption of Phosphatidylserine Synthesis or Trafficking Reduces Infectivity of Ebola Virus
Q59352107Drug Repurposing for Ebola Virus Disease: Principles of Consideration and the Animal Rule
Q34274569Drug discovery technologies and strategies for Machupo virus and other New World arenaviruses
Q61805800Ebola Virus Bayesian Machine Learning Models Enable New in Vitro Leads
Q41676229Ebola Virus Binding to Tim-1 on T Lymphocytes Induces a Cytokine Storm.
Q59355029Ebola Virus Shed Glycoprotein Triggers Differentiation, Infection, and Death of Monocytes Through Toll-Like Receptor 4 Activation
Q57059911Ebola viral dynamics in nonhuman primates provides insights into virus immuno-pathogenesis and antiviral strategies
Q36827365Ebola virus does not block apoptotic signaling pathways.
Q33758034Ebola virus glycoprotein directly triggers T lymphocyte death despite of the lack of infection
Q40454256Ebola virus infection of human PBMCs causes massive death of macrophages, CD4 and CD8 T cell sub-populations in vitro.
Q24301618Ebola virus uses clathrin-mediated endocytosis as an entry pathway
Q40287482Ebolavirus requires acid sphingomyelinase activity and plasma membrane sphingomyelin for infection
Q35601783Evaluation of perceived threat differences posed by filovirus variants
Q28554599Evaluation of the Activity of Lamivudine and Zidovudine against Ebola Virus
Q35038226Evaluation of the potential impact of Ebola virus genomic drift on the efficacy of sequence-based candidate therapeutics
Q24568225FDA-approved selective estrogen receptor modulators inhibit Ebola virus infection.
Q27490384FGI-104: a broad-spectrum small molecule inhibitor of viral infection
Q34375243Filovirus replication and transcription
Q40066603GS-5734 and its parent nucleoside analog inhibit Filo-, Pneumo-, and Paramyxoviruses
Q40054473Generation of Recombinant Ebola Viruses Using Reverse Genetics
Q60031608High Throughput and Computational Repurposing for Neglected Diseases
Q47550612High degree of correlation between Ebola virus BSL-4 neutralization assays and pseudotyped VSV BSL-2 fluorescence reduction neutralization test
Q35748556High-Throughput Minigenome System for Identifying Small-Molecule Inhibitors of Ebola Virus Replication
Q38287391Homo-oligomerization facilitates the interferon-antagonist activity of the ebolavirus VP35 protein.
Q42021625Human parainfluenza virus type 3 (HPIV-3): construction and rescue of an infectious, recombinant virus expressing the enhanced green fluorescent protein (EGFP).
Q39177100Identification of a broad-spectrum inhibitor of viral RNA synthesis: validation of a prototype virus-based approach
Q33712786Identification of novel cellular targets for therapeutic intervention against Ebola virus infection by siRNA screening
Q40256077Identification of two amino acid residues on Ebola virus glycoprotein 1 critical for cell entry
Q27681581In Silico Derived Small Molecules Bind the Filovirus VP35 Protein and Inhibit Its Polymerase Cofactor Activity
Q34717930Infection of naive target cells with virus-like particles: implications for the function of ebola virus VP24.
Q24294470Infectious Lassa virus, but not filoviruses, is restricted by BST-2/tetherin
Q36484103Inhibition of IRF-3 activation by VP35 is critical for the high level of virulence of ebola virus
Q28281834Inhibition of heat-shock protein 90 reduces Ebola virus replication
Q38719410Innate Immune Responses of Bat and Human Cells to Filoviruses: Commonalities and Distinctions
Q28550929Interferon-γ Inhibits Ebola Virus Infection
Q34519919In vitro antiviral activity of adenosine analog NITD008 against tick-borne flaviviruses
Q21090770Lectin-dependent enhancement of Ebola virus infection via soluble and transmembrane C-type lectin receptors
Q40054463Luciferase-Expressing Ebolaviruses as Tools for Screening of Antivirals
Q35132756Mechanism of human antibody-mediated neutralization of Marburg virus
Q36650409Minigenomes, transcription and replication competent virus-like particles and beyond: reverse genetics systems for filoviruses and other negative stranded hemorrhagic fever viruses
Q90554819Modeling favipiravir antiviral efficacy against emerging viruses: from animal studies to clinical trials
Q24696012Monitoring of Ebola Virus Makona Evolution through Establishment of Advanced Genomic Capability in Liberia
Q56897586Multifunctional Pan-ebolavirus Antibody Recognizes a Site of Broad Vulnerability on the Ebolavirus Glycoprotein
Q28486226Multiple cationic amphiphiles induce a Niemann-Pick C phenotype and inhibit Ebola virus entry and infection
Q40471665Permeabilization of the plasma membrane by Ebola virus GP2.
Q37168685Polyamines and Hypusination Are Required for Ebolavirus Gene Expression and Replication
Q35121478Probing the virus host interaction in high containment: an approach using pooled short hairpin RNA
Q30230272Progress towards the treatment of Ebola haemorrhagic fever
Q57167222Protein Phosphatase 1-Targeting Small-Molecule C31 Inhibits Ebola Virus Replication
Q24562099Pyridinyl imidazole inhibitors of p38 MAP kinase impair viral entry and reduce cytokine induction by Zaire ebolavirus in human dendritic cells
Q35557172Recombinant Marburg virus expressing EGFP allows rapid screening of virus growth and real-time visualization of virus spread
Q28387786Replication-competent fluorescent-expressing influenza B virus
Q92734169Reporter Assays for Ebola Virus Nucleoprotein Oligomerization, Virion-Like Particle Budding, and Minigenome Activity Reveal the Importance of Nucleoprotein Amino Acid Position 111
Q34301750Rescue of recombinant Marburg virus from cDNA is dependent on nucleocapsid protein VP30
Q34342378Retinazone inhibits certain blood-borne human viruses including Ebola virus Zaire.
Q58588686Reverse Genetic Approaches for the Generation of Recombinant Zika Virus
Q40056806Reverse Genetics Systems for Filoviruses
Q41936032Reverse Genetics of Filoviruses
Q34717075Reverse genetic generation of recombinant Zaire Ebola viruses containing disrupted IRF-3 inhibitory domains results in attenuated virus growth in vitro and higher levels of IRF-3 activation without inhibiting viral transcription or replication
Q38244444Reverse genetics systems as tools for the development of novel therapies against filoviruses
Q35641426Role of EXT1 and Glycosaminoglycans in the Early Stage of Filovirus Entry
Q56982857Role of Transmembrane Protein 16F in the Incorporation of Phosphatidylserine Into Budding Ebola Virus Virions
Q34044597Role of protein phosphatase 1 in dephosphorylation of Ebola virus VP30 protein and its targeting for the inhibition of viral transcription
Q37036794Role of the phosphatidylserine receptor TIM-1 in enveloped-virus entry.
Q24329155Small molecule inhibitors reveal Niemann-Pick C1 is essential for Ebola virus infection
Q57291140Staufen1 Interacts with Multiple Components of the Ebola Virus Ribonucleoprotein and Enhances Viral RNA Synthesis
Q51782676Susceptibility of paramyxoviruses and filoviruses to inhibition by 2'-monofluoro- and 2'-difluoro-4'-azidocytidine analogs.
Q34971349Tackling Ebola: new insights into prophylactic and therapeutic intervention strategies
Q90401053Targeting the Non-catalytic RVxF Site of Protein Phosphatase-1 With Small Molecules for Ebola Virus Inhibition
Q52724948Testing therapeutics in cell-based assays: Factors that influence the apparent potency of drugs.
Q36216308The Ebola Interferon Inhibiting Domains Attenuate and Dysregulate Cell-Mediated Immune Responses.
Q34042182The cyanobacterial lectin scytovirin displays potent in vitro and in vivo activity against Zaire Ebola virus
Q36085668The evolving field of biodefence: therapeutic developments and diagnostics
Q36978855The lack of maturation of Ebola virus-infected dendritic cells results from the cooperative effect of at least two viral domains
Q34516555Therapeutic efficacy of the small molecule GS-5734 against Ebola virus in rhesus monkeys
Q92154130Therapeutic strategies to target the Ebola virus life cycle
Q88978790Topoisomerase III-beta is required for efficient replication of positive-sense RNA viruses
Q33568288Virus nomenclature below the species level: a standardized nomenclature for filovirus strains and variants rescued from cDNA
Q38610343Whole-genome expression profiling reveals that inhibition of host innate immune response pathways by Ebola virus can be reversed by a single amino acid change in the VP35 protein

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