Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli

scientific article published in September 1988

Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1016/0378-1119(88)90440-4
P698PubMed publication ID3069586

P2093author name stringE Amann
K J Abel
B Ochs
P433issue2
P407language of work or nameEnglishQ1860
P921main subjectEscherichia coliQ25419
P304page(s)301-315
P577publication date1988-09-01
P1433published inGeneQ5531065
P1476titleTightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli
P478volume69

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Q44443420Characterisation of mutations in GroES that allow GroEL to function as a single ring
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Q36112921Characterization of Saccharopolyspora erythraea cytochrome P-450 genes and enzymes, including 6-deoxyerythronolide B hydroxylase
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Q35626192Characterization of a second lysine decarboxylase isolated from Escherichia coli
Q39797754Characterization of biphenyl catabolic genes of gram-positive polychlorinated biphenyl degrader Rhodococcus sp. strain RHA1.
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Q41598673Characterization of the reverse transcriptase of 1731, a Drosophila melanogaster retrotransposon
Q42578933Characterization of three new Azotobacter vinelandii alginate lyases, one of which is involved in cyst germination
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Q39798605Characterizing the hexose-6-phosphate transport system of Vibrio cholerae, a utilization system for carbon and phosphate sources
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Q24303996Cloning and characterization of the cDNA encoding human adenylosuccinate synthetase
Q28492354Cloning and characterization of uronate dehydrogenases from two pseudomonads and Agrobacterium tumefaciens strain C58
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Q74618502Cloning and nucleotide sequencing of the secA gene from coryneform bacteria
Q34412293Cloning and sequencing of a gene coding for a novel dextransucrase from Leuconostoc mesenteroides NRRL B-1299 synthesizing only alpha (1-6) and alpha (1-3) linkages
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Q38841844Determinants of Proteolysis and Cell-Binding for the Shigella flexneri Cytotoxin, SigA.
Q54459232Determinants of chemoreceptor cluster formation in Escherichia coli.
Q36890483Determination of the physiological dimer interface of the PhoQ sensor domain
Q50528263Developing an extended genomic engineering approach based on recombineering to knock-in heterologous genes to Escherichia coli genome.
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Q41669232Development of a novel uric-acid-responsive regulatory system in Escherichia coli
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Q39696482Domain organization of the MscS mechanosensitive channel of Escherichia coli
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Q37372414EGFR-targeted Chimeras of Pseudomonas ToxA released into the extracellular milieu by attenuated Salmonella selectively kill tumor cells
Q38337986Effect of Leuconostoc mesenteroides NRRL B-512F dextransucrase carboxy-terminal deletions on dextran and oligosaccharide synthesis
Q41037493Effect of overexpressing nhaA and nhaR on sodium tolerance and lactate production in Escherichia coli
Q45071360Effect of plasmid copy number and lac operator sequence on antibiotic-free plasmid selection by operator-repressor titration in Escherichia coli
Q59797231Effect of β-lactam antibiotic resistance gene expression on the radio-resistance profile of O157:H7
Q41871881Effects of Friedreich's ataxia (GAA)n*(TTC)n repeats on RNA synthesis and stability
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Q35549414Effects of antibodies against cell surface protein antigen PAc-glucosyltransferase fusion proteins on glucan synthesis and cell adhesion of Streptococcus mutans
Q34042025Effects of receptor modification and temperature on dynamics of sensory complexes in Escherichia coli chemotaxis
Q36627949Effects of tRNA modification on translational accuracy depend on intrinsic codon-anticodon strength
Q28604061Engineering Escherichia coli for Microbial Production of Butanone
Q37338370Engineering Escherichia coli for production of C₁₂-C₁₄ polyhydroxyalkanoate from glucose.
Q88647920Engineering a short, aldolase-based pathway for (R)-1,3-butanediol production in Escherichia coli
Q39487425Engineering hydrogen sulfide production and cadmium removal by expression of the thiosulfate reductase gene (phsABC) from Salmonella enterica serovar typhimurium in Escherichia coli
Q28828554Engineering of a microbial coculture of Escherichia coli strains for the biosynthesis of resveratrol
Q54326929Engineering of an N-acetylneuraminic acid synthetic pathway in Escherichia coli.
Q37274915Engineering of recombinant Escherichia coli cells co-expressing poly-γ-glutamic acid (γ-PGA) synthetase and glutamate racemase for differential yielding of γ-PGA
Q48051532Enhanced expression in tobacco of the gene encoding green fluorescent protein by modification of its codon usage
Q46713487Enhancement of succinate production by metabolically engineered Escherichia coli with co-expression of nicotinic acid phosphoribosyltransferase and pyruvate carboxylase
Q34795539Environmental dependence of genetic constraint
Q50136458Enzymatic characterization of FliI. An ATPase involved in flagellar assembly in Salmonella typhimurium
Q54596157Enzymatic properties of chorismate synthase isozymes of tomato (Lycopersicon esculentum Mill.).
Q39848143Escherichia coli cells expressing a mutant glyV (glycine tRNA) gene have a UVM-constitutive phenotype: implications for mechanisms underlying the mutA or mutC mutator effect
Q34541189Essential role of YopD in inhibition of the respiratory burst of macrophages by Yersinia enterocolitica
Q64359297Evaluation of Crimean-Congo Hemorrhagic Fever Orthonairovirus AviTagged Nucleoprotein for Potential Application in Diagnosis
Q28365504Evidence for a novel GTPase priming step in the SRP protein targeting pathway
Q34430675Evolutionary expansion of a regulatory network by counter-silencing
Q42495371Excretion of the anti-sigma factor through a flagellar substructure couples flagellar gene expression with flagellar assembly in Salmonella typhimurium
Q35905616ExoMeg1: a new exonuclease from metagenomic library.
Q36228535Exploring the DNA mimicry of the Ocr protein of phage T7.
Q38337981Exploring the role of integral membrane proteins in ATP-binding cassette transporters: analysis of a collection of MalG insertion mutants.
Q74292210Expression of Trigonopsis variabilis D-amino acid oxidase gene in Escherichia coli and characterization of its inactive mutants
Q39661868Expression of an anaplerotic enzyme, pyruvate carboxylase, improves recombinant protein production in Escherichia coli
Q47918087Expression of constitutive and tissue-specific acyl carrier protein isoforms in Arabidopsis
Q39494805Expression of the Methanobacterium thermoautotrophicum hpt gene, encoding hypoxanthine (Guanine) phosphoribosyltransferase, in Escherichia coli.
Q44311934Expression of the human blood coagulation protein factor XIIIa in Saccharomyces cerevisiae: dependence of the expression levels from host-vector systems and medium conditions
Q43805029Expression of the measles virus nucleoprotein gene in Escherichia coli and assembly of nucleocapsid-like structures
Q44010655Expression, purification and biochemical characterisation of the human immunodeficiency virus 1 nef gene product.
Q27655819Extensive DNA mimicry by the ArdA anti-restriction protein and its role in the spread of antibiotic resistance
Q39587612Fine-tuning of chemotactic response in E. coli determined by high-throughput capillary assay
Q90629826Flagellum-Mediated Mechanosensing and RflP Control Motility State of Pathogenic Escherichia coli
Q44639518Flavodoxin overexpression confers tolerance to oxidative stress in beneficial soil bacteria and improves survival in the presence of the herbicides paraquat and atrazine.
Q38503225FliT acts as an anti-FlhD2C2 factor in the transcriptional control of the flagellar regulon in Salmonella enterica serovar typhimurium
Q43182335FljA-mediated posttranscriptional control of phase 1 flagellin expression in flagellar phase variation of Salmonella enterica serovar Typhimurium
Q35131867Fluorescence-based monitoring of ribosome assembly landscapes
Q47442864Folding of a bacterial integral outer membrane protein is initiated in the periplasm
Q43162053Formate hydrogen lyase mediates stationary-phase deacidification and increases survival during sugar fermentation in acetoin-producing enterobacteria.
Q54695513Frameshifting by eukaryotic ribosomes during expression of Escherichia coli release factor 2
Q39323774Functional analysis of the group 4 late embryogenesis abundant proteins reveals their relevance in the adaptive response during water deficit in Arabidopsis
Q37451542Functional and biochemical analysis of the Chlamydia trachomatis ligase MurE.
Q40901972Functional dissection of Escherichia coli trigger factor: unraveling the function of individual domains
Q34827120Functional dissection of the transmitter module of the histidine kinase NtrB in Escherichia coli
Q38348949Functional domains of Agrobacterium tumefaciens single-stranded DNA-binding protein VirE2.
Q36367901Functional domains of the AraC protein
Q48042261Functional role of the type 1 pilus rod structure in mediating host-pathogen interactions.
Q41951429Furfural inhibits growth by limiting sulfur assimilation in ethanologenic Escherichia coli strain LY180.
Q50465502Gene fliA encodes an alternative sigma factor specific for flagellar operons in Salmonella typhimurium.
Q33254725General mutagenesis of F plasmid TraI reveals its role in conjugative regulation
Q40793356Genetic and molecular analyses of the interaction between the flagellum-specific sigma and anti-sigma factors in Salmonella typhimurium
Q47294854Genetic drift and selection in many-allele range expansions
Q39932051Genetic evidence that the XylS regulator of the Pseudomonas TOL meta operon controls the Pm promoter through weak DNA-protein interactions
Q42575874GlcNAc-6P levels modulate the expression of Curli fibers by Escherichia coli
Q46590391Globin-coupled sensors and protoglobins share a common signaling mechanism
Q38770967Glu-311 in External Loop 4 of the Sodium/Proline Transporter PutP Is Crucial for External Gate Closure
Q39836003Gratuitous overexpression of genes in Escherichia coli leads to growth inhibition and ribosome destruction
Q41770558Growth Inhibition by External Potassium of Escherichia coli Lacking PtsN (EIIANtr) Is Caused by Potassium Limitation Mediated by YcgO.
Q74191649Growth phase dependent stop codon readthrough and shift of translation reading frame in Escherichia coli
Q45002013Growth-rate recovery of Escherichia coli cultures carrying a multicopy plasmid, by engineering of the pentose-phosphate pathway
Q41908196HetR-dependent and -independent expression of heterocyst-related genes in an Anabaena strain overproducing the NtcA transcription factor.
Q42258994High pressure activation of the Mrr restriction endonuclease in Escherichia coli involves tetramer dissociation
Q33743392High-efficiency scarless genetic modification in Escherichia coli by using lambda red recombination and I-SceI cleavage
Q33758023Highly conserved type 1 pili promote enterotoxigenic E. coli pathogen-host interactions
Q40382562Host restriction of Salmonella enterica serotype Typhi is not caused by functional alteration of SipA, SopB, or SopD.
Q27317194How Obstacles Perturb Population Fronts and Alter Their Genetic Structure
Q34405466Identification and analysis of the putative pentose sugar efflux transporters in Escherichia coli
Q39841503Identification and characterization of BpH2, a novel histone H1 homolog in Bordetella pertussis.
Q34633732Identification and functional characterization of an α-amylase with broad temperature and pH stability from Paenibacillus sp.
Q37127916Identification and isolation of the polyferredoxin from Methanobacterium thermoautotrophicum strain delta H
Q41564912Identification of Genes Required for Growth of Escherichia coli MG1655 at Moderately Low pH.
Q38998949Identification of Unannotated Small Genes in Salmonella
Q24594435Identification of a chitin-induced small RNA that regulates translation of the tfoX gene, encoding a positive regulator of natural competence in Vibrio cholerae
Q36759623Identification of a major soluble protein in mitochondria from nonphotosynthetic tissues as NAD-dependent formate dehydrogenase
Q43026749Identification of a suppressor gene for the arginine-auxotrophic argJ mutation in Corynebacterium glutamicum
Q35352832Identification of inhibitors of a bacterial sigma factor using a new high-throughput screening assay
Q33293921Identification of isopentenol biosynthetic genes from Bacillus subtilis by a screening method based on isoprenoid precursor toxicity
Q37204550Identification of migR, a regulatory element of the Francisella tularensis live vaccine strain iglABCD virulence operon required for normal replication and trafficking in macrophages.
Q35879244Identification of the L,D-transpeptidases responsible for attachment of the Braun lipoprotein to Escherichia coli peptidoglycan
Q48071441Identification of the Magnesium-binding Domain of the High-affinity ATP-binding Site of the Bacillus subtilis and Escherichia coli SecA Protein
Q64910270Identification of the Thioredoxin Partner of Vitamin K Epoxide Reductase in Mycobacterial Disulfide Bond Formation.
Q71070048Identification of the repressor subdomain within the signal reception module of the prokaryotic enhancer-binding protein XylR of Pseudomonas putida
Q28854144Identification of two prpDBC gene clusters in Corynebacterium glutamicum and their involvement in propionate degradation via the 2-methylcitrate cycle
Q48668624Implementing an OR-NOT (ORN) logic gate with components of the SOS regulatory network of Escherichia coli
Q44075286Improved partitioning in aqueous two-phase system of tyrosine-tagged recombinant lactate dehydrogenase
Q78029859Improved production of poly(4-hydroxybutyrate) by Comamonas acidovorans and its freeze-fracture morphology
Q39527268Improvement in K+-limited growth rate associated with expression of the N-terminal fragment of one subunit (KdpA) of the multisubunit Kdp transporter in Escherichia coli
Q44772310Improving poly-3-hydroxybutyrate production in Escherichia coli by combining the increase in the NADPH pool and acetyl-CoA availability
Q43685884In vivo and in vitro lipidation of recombinant immunogens for direct iscom incorporation
Q35588041In vivo induction kinetics of the arabinose promoters in Escherichia coli
Q33290865In vivo oligomerization of the F conjugative coupling protein TraD.
Q42339316In vivo plug-and-play: a modular multi-enzyme single-cell catalyst for the asymmetric amination of ketoacids and ketones
Q49790370In vivo repressed genes of Vibrio cholerae reveal inverse requirements of an H+/Cl- transporter along the gastrointestinal passage
Q34362511In vivo selection of lethal mutations reveals two functional domains in arginyl-tRNA synthetase
Q41874086Increased furfural tolerance due to overexpression of NADH-dependent oxidoreductase FucO in Escherichia coli strains engineered for the production of ethanol and lactate
Q54356282Increased production of succinic acid in Escherichia coli by overexpression of malate dehydrogenase.
Q43030486Increased tolerance to thermal inactivation of oxygen evolution in spinach Photosystem II membranes by substitution of the extrinsic 33-kDa protein by its homologue from a thermophilic cyanobacterium
Q38860249Increasing the production of (R)-3-hydroxybutyrate in recombinant Escherichia coli by improved cofactor supply
Q44009176Influences on translation initiation and early elongation by the messenger RNA region flanking the initiation codon at the 3' side
Q90577503Inhibition of Pseudomonas aeruginosa and Mycobacterium tuberculosis disulfide bond forming enzymes
Q33591624Inhibition of bacterial disulfide bond formation by the anticoagulant warfarin
Q33991270Inhibition of homologous recombination by the plasmid MucA'B complex
Q30649858Inhibition of translation and cell growth by minigene expression
Q35837534Inorganic polyphosphate kinase and adenylate kinase participate in the polyphosphate:AMP phosphotransferase activity of Escherichia coli
Q39845995Insertion mutagenesis of the lac repressor and its implications for structure-function analysis
Q35832258Insertional gene synthesis, a novel method of assembling consecutive DNA sequences within specific sites in plasmids. Construction of the HIV-1 tat gene
Q33481550Integrase-directed recovery of functional genes from genomic libraries
Q42780084Integrated regulation of acetoin fermentation by quorum sensing and pH in Serratia plymuthica RVH1
Q34042512Integration host factor suppresses promiscuous activation of the sigma 54-dependent promoter Pu of Pseudomonas putida
Q41948795Interaction of Streptococcus mutans YidC1 and YidC2 with translating and nontranslating ribosomes.
Q40278902Interresidual distance determination by four-pulse double electron-electron resonance in an integral membrane protein: the Na+/proline transporter PutP of Escherichia coli
Q42157317Intersubunit interaction and catalytic activity of catechol 2,3-dioxygenases.
Q33454924Involvement of RNA-binding protein Hfq in the osmotic-response regulation of invE gene expression in Shigella sonnei
Q57936199Involvement of the FtsH (HflB) protease in the activity of sigma54 promoters
Q24613961Islet cell autoantigen 69 kD (ICA69). Molecular cloning and characterization of a novel diabetes-associated autoantigen
Q41783807Isolation and overexpression in Escherichia coli of the flavodoxin gene from Anabaena PCC 7119
Q33727049Isolation and purification of two novel streptomycete RNase inhibitors, SaI14 and SaI20, and cloning, sequencing, and expression in Escherichia coli of the gene coding for SaI14.
Q39842573Isolation, purification, and in vitro characterization of recessive-lethal-mutant RNA polymerases from Escherichia coli
Q24533325L-arginine recognition by yeast arginyl-tRNA synthetase
Q44134061L23 protein functions as a chaperone docking site on the ribosome
Q90134195Learning the pattern of epistasis linking genotype and phenotype in a protein
Q30513626Loss of FlhE in the flagellar Type III secretion system allows proton influx into Salmonella and Escherichia coli
Q35620140MalFGK complex assembly and transport and regulatory characteristics of MalK insertion mutants
Q38291936MalY of Escherichia coli is an enzyme with the activity of a beta C-S lyase (cystathionase)
Q41845448Malonic semialdehyde reductase, succinic semialdehyde reductase, and succinyl-coenzyme A reductase from Metallosphaera sedula: enzymes of the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle in Sulfolobales
Q39936764Maltose and maltotriose can be formed endogenously in Escherichia coli from glucose and glucose-1-phosphate independently of enzymes of the maltose system
Q43814794Measuring editing activity and identifying cytidine-to-uridine mRNA editing factors in cells and biochemical isolates
Q40890748Mechanisms of transcription-replication collisions in bacteria
Q49791482Membrane curvature and the Tol-Pal complex determine polar localization of the chemoreceptor Tar in E. coli.
Q35530116Membrane stresses induced by overproduction of free fatty acids in Escherichia coli
Q39536097Metabolic analysis of Escherichia coli in the presence and absence of the carboxylating enzymes phosphoenolpyruvate carboxylase and pyruvate carboxylase
Q39358944Metabolic and transcriptional response of Escherichia coli with a NADP(+)-dependent glyceraldehyde 3-phosphate dehydrogenase from Streptococcus mutans.
Q45002015Metabolic engineering and protein directed evolution increase the yield of L-phenylalanine synthesized from glucose in Escherichia coli
Q35693233Metabolic engineering for the high-yield production of isoprenoid-based C₅ alcohols in E. coli.
Q38607784Metabolic engineering of Escherichia coli for L-tyrosine production by expression of genes coding for the chorismate mutase domain of the native chorismate mutase-prephenate dehydratase and a cyclohexadienyl dehydrogenase from Zymomonas mobilis.
Q83932151Metabolic engineering of Escherichia coli for improving L-3,4-dihydroxyphenylalanine (L-DOPA) synthesis from glucose
Q54353209Metabolic engineering of Escherichia coli for α-farnesene production.
Q42862833Metabolic engineering of Escherichia coli to optimize melanin synthesis from glucose
Q36732678Metal selectivity by the virulence-associated yersiniabactin metallophore system
Q90634836Mevalonate production from ethanol by direct conversion through acetyl-CoA using recombinant Pseudomonas putida, a novel biocatalyst for terpenoid production
Q34646096Mirror image alternative interaction patterns of the same tRNA with either class I arginyl-tRNA synthetase or class II aspartyl-tRNA synthetase
Q33647888Mobility of cytoplasmic, membrane, and DNA-binding proteins in Escherichia coli
Q34562524Modulating membrane composition alters free fatty acid tolerance in Escherichia coli
Q41885642Modulation of DNA damage tolerance in Escherichia coli recG and ruv strains by mutations affecting PriB, the ribosome and RNA polymerase
Q39265512Modulation of Rho-dependent transcription termination in Escherichia coli by the H-NS family of proteins
Q36107718Molecular analysis of the Trichosporon cutaneum DSM 70698 argA gene and its use for DNA-mediated transformations
Q39868463Molecular and proteome analyses highlight the importance of the Cpx envelope stress system for acid stress and cell wall stability in Escherichia coli.
Q28214985Molecular characterization of long direct repeat (LDR) sequences expressing a stable mRNA encoding for a 35-amino-acid cell-killing peptide and a cis-encoded small antisense RNA in Escherichia coli
Q39899611Molecular characterization of the Salmonella typhimurium flhB operon and its protein products
Q35984111Molecular cloning and expression of an isomalto-dextranase gene from Arthrobacter globiformis T6.
Q37883424Molecular cloning, sequence analysis and functional characterization of the gene kdsA, encoding 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase of Chlamydia psittaci 6BC.
Q50141336Molecular dissection of the flagellum-specific anti-sigma factor, FlgM, of Salmonella typhimurium
Q40792278Molecular mechanism for the operation of nitrogen control in cyanobacteria
Q43560899Molecular mechanism of magnet formation in bacteria
Q40716008Molecular mechanisms of xylose utilization by Pseudomonas fluorescens: overlapping genetic responses to xylose, xylulose, ribose and mannitol.
Q35280194Molecular ruler determines needle length for the Salmonella Spi-1 injectisome.
Q34479236Monitoring bacterial chemotaxis by using bioluminescence resonance energy transfer: absence of feedback from the flagellar motors
Q56894122MreB polymers and curvature localization are enhanced by RodZ and predict E. coli's cylindrical uniformity
Q35172182Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system
Q40690983Multiple effects of S13 in modulating the strength of intersubunit interactions in the ribosome during translation
Q39538569Multiple factors independently regulate hilA and invasion gene expression in Salmonella enterica serovar typhimurium
Q100395110Mutational analysis of Escherichia coli GreA protein reveals new functional activity independent of antipause and lethal when overexpressed
Q44145338Mutational analysis of bacteriophage T4 AsiA: involvement of N- and C-terminal regions in binding to sigma(70) of Escherichia coli in vivo
Q40654619Mutational and kinetic analyses of the GTPase-activating protein (GAP)-p21 interaction: the C-terminal domain of GAP is not sufficient for full activity
Q36970526Mutations in the Escherichia coli ribosomal protein L22 selectively suppress the expression of a secreted bacterial virulence factor.
Q40214494Mutations in the Yersinia pseudotuberculosis type III secretion system needle protein, YscF, that specifically abrogate effector translocation into host cells
Q33937947Mutations of Ha-ras p21 that define important regions for the molecular mechanism of the SDC25 C-domain, a guanine nucleotide dissociation stimulator
Q41900153Mutations of the domain forming the dimeric interface of the ArdA protein affect dimerization and antimodification activity but not antirestriction activity
Q34498164Mycobacteriophage D29 holin C-terminal region functionally assists in holin aggregation and bacterial cell death
Q55450202N-Acetyl-glucosamine influences the biofilm formation of Escherichia coli.
Q33215307NAD+-dependent DNA Ligase (Rv3014c) from Mycobacterium tuberculosis. Crystal structure of the adenylation domain and identification of novel inhibitors
Q38339751Negative transcriptional regulation of a positive regulator: the expression of malT, encoding the transcriptional activator of the maltose regulon of Escherichia coli, is negatively controlled by Mlc.
Q28365921New insights into the activation of o-xylene biodegradation in Pseudomonas stutzeri OX1 by pathway substrates
Q44357465New insights on transcriptional responses of genes involved in carbon central metabolism, respiration and fermentation to low ATP levels in Escherichia coli
Q43977334Novel gene products associated with NdhD3/D4-containing NDH-1 complexes are involved in photosynthetic CO2 hydration in the cyanobacterium, Synechococcus sp. PCC7942.
Q33744080Novel organization of the genes for phthalate degradation from Burkholderia cepacia DBO1.
Q38931137Novel production of terpenoids in Escherichia coli and activities against breast cancer cell lines
Q28343699Novel topology of BfpE, a cytoplasmic membrane protein required for type IV fimbrial biogenesis in enteropathogenic Escherichia coli
Q70683771Nucleotide sequence of attP and cos sites of phage CTX and expression of cytotoxin in Pseudomonas aeruginosa PA158
Q36102196Nucleotide sequences and heterologous expression of tcmG and tcmP, biosynthetic genes for tetracenomycin C in Streptomyces glaucescens
Q35799354Optimizing Production of Antigens and Fabs in the Context of Generating Recombinant Antibodies to Human Proteins
Q51119129Optimum melanin production using recombinant Escherichia coli.
Q41466529Oral immunisation with live aroA attenuated Salmonella enterica serovar Typhimurium expressing the Yersinia pestis V antigen protects mice against plague
Q30874371Origins of minigene-dependent growth inhibition in bacterial cells
Q34270957Osmotic stress mechanically perturbs chemoreceptors in Escherichia coli
Q50535124Overexpression in E. coli of the complete petH gene product from Anabaena: purification and properties of a 49 kDa ferredoxin-NADP+ reductase.
Q39840207Overexpression, purification, and characterization of UDP-N-acetylmuramyl:L-alanine ligase from Escherichia coli
Q39800640Overproduction of recombinant ribulose 1,5-bisphosphate carboxylase/oxygenase from Synechococcus sp. strain PCC6301 in glucose-controlled high-cell-density fermentations by Escherichia coli K-12
Q71845700Over‐production, Purification and Properties of the Uridine‐diphosphate‐N ‐Acetylmuramate: l‐alanine Ligase from Escherichia coli
Q37574012PAS domain of the Aer redox sensor requires C-terminal residues for native-fold formation and flavin adenine dinucleotide binding
Q32062093PCR isolation of catechol 2,3-dioxygenase gene fragments from environmental samples and their assembly into functional genes.
Q47318459PHA synthase activity controls the molecular weight and polydispersity of polyhydroxybutyrate in vivo
Q35166061PapD-like chaperones provide the missing information for folding of pilin proteins
Q24549568Peptidoglycan-hydrolyzing activity of the FlgJ protein, essential for flagellar rod formation in Salmonella typhimurium
Q91701461Perforin-2 Breaches the Envelope of Phagocytosed Bacteria Allowing Antimicrobial Effectors Access to Intracellular Targets
Q35806137Perturbation of the oxidizing environment of the periplasm stimulates the PhoQ/PhoP system in Escherichia coli
Q40634273Phospho-N-acetyl-muramyl-pentapeptide translocase from Escherichia coli: catalytic role of conserved aspartic acid residues
Q24563337Phosphorylated BvgA is sufficient for transcriptional activation of virulence-regulated genes in Bordetella pertussis
Q34279829Phosphotransferase-mediated transport of the osmolyte 2-O-alpha-mannosyl-D-glycerate in Escherichia coli occurs by the product of the mngA (hrsA) gene and is regulated by the mngR (farR) gene product acting as repressor
Q34440901Pilicide ec240 disrupts virulence circuits in uropathogenic Escherichia coli
Q47866126Pilot scale recovery of recombinant annexin V from unclarified escherichia coli homogenate using expanded bed adsorption
Q35620234Plasmid virulence gene expression induced by short-chain fatty acids in Salmonella dublin: identification of rpoS-dependent and rpo-S-independent mechanisms
Q55432305Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance.
Q39373164Precise targeted integration by a chimaeric transposase zinc-finger fusion protein.
Q36883939Prochlorococcus can use the Pro1404 transporter to take up glucose at nanomolar concentrations in the Atlantic Ocean.
Q35008660Production of cinnamic and p-hydroxycinnamic acid from sugar mixtures with engineered Escherichia coli
Q44038146Production of functional single-chain Fv antibodies in the cytoplasm of Escherichia coli
Q28303073Production of glucaric acid from a synthetic pathway in recombinant Escherichia coli
Q24628546Production of glycoprotein vaccines in Escherichia coli
Q43733020Production of jet fuel precursor monoterpenoids from engineered Escherichia coli.
Q28661058Production of medium chain length fatty alcohols from glucose in Escherichia coli
Q48568320Production of recombinant buffalo (Bubalus bubalis) and goat (Capra hircus) growth hormones from genetically modified E. coli strains
Q57298358Production of succinate by recombinant using acetate as the sole carbon source
Q47726315Promiscuous Defluorinating Enoyl-CoA Hydratases/Hydrolases Allow for Complete Anaerobic Degradation of 2-Fluorobenzoate
Q40146914Protection of Aotus monkeys from malaria infection by immunization with recombinant hybrid proteins
Q35410420Protection of immunocompromised mice against lethal infection with Pseudomonas aeruginosa by active or passive immunization with recombinant P. aeruginosa outer membrane protein F and outer membrane protein I fusion proteins
Q58049566Protein aggregates encode epigenetic memory of stressful encounters in individual Escherichia coli cells
Q36609149Protein exchange dynamics at chemoreceptor clusters in Escherichia coli
Q54547515Protein nucleation and crystallization by homologous protein thin film template.
Q28818720Proteins adopt functionally active conformations after type III secretion
Q57108353Proteolysis of ToxR is controlled by cysteine-thiol redox state and bile salts in Vibrio cholerae
Q71874388Pseudomonas aeruginosa cytotoxin: the Asp197-Gly-Asp-Tyr-His-Tyr-His-Tyr202 containing loop is critical for plasma membrane binding
Q39477683Pseudomonas aeruginosa reveals high intrinsic resistance to penem antibiotics: penem resistance mechanisms and their interplay
Q28486593Purification and characterization of recombinant catalase-peroxidase, which confers isoniazid sensitivity in Mycobacterium tuberculosis
Q44985543Purification of E. coli-expressed HIS-tagged hepatitis B core antigen by Ni2+ -chelate affinity chromatography
Q46985897Quantitative colorimetric measurement of cellulose degradation under microbial culture conditions
Q34532728Quantitative, non-disruptive monitoring of transcription in single cells with a broad-host range GFP-luxCDABE dual reporter system
Q90043873Quorum Sensing and Metabolic State of the Host Control Lysogeny-Lysis Switch of Bacteriophage T1
Q44635551Qβ-Phage Resistance by Deletion of the Coiled-coil Motif in Elongation Factor Ts
Q40285768Rapid customised operon assembly by yeast recombinational cloning
Q41997423Rational stabilization of complex proteins: a divide and combine approach
Q42126946Recognition of DNA by the helix-turn-helix global regulatory protein Lrp is modulated by the amino terminus
Q36107477Recognition specificity of the duplicated segments present in Clostridium thermocellum endoglucanase CelD and in the cellulosome-integrating protein CipA.
Q39442166Recombinant Escherichia coli with sulfide:quinone oxidoreductase and persulfide dioxygenase rapidly oxidises sulfide to sulfite and thiosulfate via a new pathway
Q46344496Reconstitution of mature plastocyanin from precursor apo-plastocyanin expressed in Escherichia coli
Q40926431Regulation of the Min Cell Division Inhibition Complex by the Rcs Phosphorelay in Proteus mirabilis
Q38293420Regulation of the fimB promoter: a case of differential regulation by ppGpp and DksA in vivo.
Q54289013Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants.
Q54697323Relationship of primary structure to functional properties of the cytotoxin protein from Pseudomonas aeruginosa.
Q36461216Replacement of Y730 and Y731 in the alpha2 subunit of Escherichia coli ribonucleotide reductase with 3-aminotyrosine using an evolved suppressor tRNA/tRNA-synthetase pair
Q41909283Response of extraintestinal pathogenic Escherichia coli to human serum reveals a protective role for Rcs-regulated exopolysaccharide colanic acid.
Q37124929Role for Skp in LptD assembly in Escherichia coli
Q35634033Role of NifS in maturation of glutamine phosphoribosylpyrophosphate amidotransferase
Q46835311Role of Ser-340 and Thr-341 in transmembrane domain IX of the Na+/proline transporter PutP of Escherichia coli in ligand binding and transport.
Q54580890Role of conserved Asn-Tyr-Asp-Tyr sequence in bacterial copper/2,4, 5-trihydroxyphenylalanyl quinone-containing histamine oxidase.
Q42572198Role of forefinger and thumb loops in production of Ψ54 and Ψ55 in tRNAs by archaeal Pus10.
Q39932319Role of the FliA-FlgM regulatory system on the transcriptional control of the flagellar regulon and flagellar formation in Salmonella typhimurium
Q33494496Role of translational coupling in robustness of bacterial chemotaxis pathway
Q34845515Salmonella chemoreceptors McpB and McpC mediate a repellent response to L-cystine: a potential mechanism to avoid oxidative conditions.
Q34119481Salmonella vaccine vectors displaying delayed antigen synthesis in vivo to enhance immunogenicity
Q33406647Secretion of flagellin by the LEE-encoded type III secretion system of enteropathogenic Escherichia coli
Q41061782Secretion of the Haemophilus influenzae HMW1 and HMW2 adhesins involves a periplasmic intermediate and requires the HMWB and HMWC proteins
Q42638850Selective Proteomic Analysis of Antibiotic-Tolerant Cellular Subpopulations in Pseudomonas aeruginosa Biofilms
Q42598040Sequence and transcript analysis of the Nco2.5 Ogura-specific fragment correlated with cytoplasmic male sterility in Brassica cybrids
Q33855829Sequestration of specific tRNA species cognate to the last sense codon of an overproduced gratuitous protein
Q40792929Serotype O:8 isolates in the Yersinia pseudotuberculosis complex have different O-antigen gene clusters and produce various forms of rough LPS.
Q31104230Single amino acid changes in AspRS reveal alternative routes for expanding its tRNA repertoire in vivo
Q34317599Site-specific recombination system encoded by toluene catabolic transposon Tn4651
Q43833690Sites important for Na+ and substrate binding in the Na+/proline transporter of Escherichia coli, a member of the Na+/solute symporter family
Q60447928Spin state transitions in Langmuir–Blodgett films of recombinant cytochrome P450scc and adrenodoxin
Q51096812Strain engineering and process optimization for enhancing the production of a thermostable steryl glucosidase in Escherichia coli.
Q54255341Strangers in the archaeal world: osmostress-responsive biosynthesis of ectoine and hydroxyectoine by the marine thaumarchaeon Nitrosopumilus maritimus.
Q42549588Structural and functional characterization of the R-modules in alginate C-5 epimerases AlgE4 and AlgE6 from Azotobacter vinelandii
Q27732831Structure and function of the glutamine phosphoribosylpyrophosphate amidotransferase glutamine site and communication with the phosphoribosylpyrophosphate site
Q72099981Structure-Function Analysis of the Ion Channel Selectivity Filter in Human Annexin V
Q47661220Study and reengineering of the binding sites and allosteric regulation of biosynthetic threonine deaminase by isoleucine and valine in Escherichia coli
Q90668008Study of measles virus recombinant proteins and their immunobiological properties
Q73009414Substrate-binding lipoprotein of the cyanobacterium Synechococcus sp. strain PCC 7942 involved in the transport of nitrate and nitrite
Q39547495Succinate dehydrogenase (Sdh) from Bradyrhizobium japonicum is closely related to mitochondrial Sdh.
Q36111845Sugar Influx Sensing by the Phosphotransferase System of Escherichia coli
Q37421160Sulfur Metabolism Pathways in Sulfobacillus acidophilus TPY, A Gram-Positive Moderate Thermoacidophile from a Hydrothermal Vent.
Q37234957Super DksAs: substitutions in DksA enhancing its effects on transcription initiation
Q39743212Synthesis of GDP-mannose and mannosylglycerate from labeled mannose by genetically engineered Escherichia coli without loss of specific isotopic enrichment
Q37589897Synthesis of arborane triterpenols by a bacterial oxidosqualene cyclase
Q87895054Synthesis of polyhydroxyalkanoates from glucose that contain medium-chain-length monomers via the reversed fatty acid β-oxidation cycle in Escherichia coli
Q36325040Systems analysis of methylerythritol-phosphate pathway flux in E. coli: insights into the role of oxidative stress and the validity of lycopene as an isoprenoid reporter metabolite.
Q54558578Tethering of the large subunits of Escherichia coli RNA polymerase.
Q39495700The A modules of the Azotobacter vinelandii mannuronan-C-5-epimerase AlgE1 are sufficient for both epimerization and binding of Ca2+.
Q36588351The Aer protein and the serine chemoreceptor Tsr independently sense intracellular energy levels and transduce oxygen, redox, and energy signals for Escherichia coli behavior
Q46676422The Azotobacter vinelandii AlgE mannuronan C-5-epimerase family is essential for the in vivo control of alginate monomer composition and for functional cyst formation.
Q77545066The Azotobacter vinelandii mannuronan C-5-epimerase AlgE1 consists of two separate catalytic domains
Q42378997The Bacteroidales produce an N-acylated derivative of glycine with both cholesterol-solubilising and hemolytic activity.
Q42793079The CRISPR/Cas immune system is an operon regulated by LeuO, H-NS, and leucine-responsive regulatory protein in Salmonella enterica serovar Typhi.
Q36434146The CreC Regulator of Escherichia coli, a New Target for Metabolic Manipulations.
Q41902961The Cyanobacterial Ribosomal-Associated Protein LrtA Is Involved in Post-Stress Survival in Synechocystis sp. PCC 6803.
Q30160163The Escherichia coli CpxA-CpxR envelope stress response system regulates expression of the porins ompF and ompC.
Q64063967The Evolution of SlyA/RovA Transcription Factors from Repressors to Countersilencers in
Q42624904The Gag polypeptides of the Drosophila 1731 retrotransposon are associated to virus-like particles and to nuclei
Q39719072The HsdR subunit of R.EcoR124II: cloning and over-expression of the gene and unexpected properties of the subunit
Q28854136The MurC ligase essential for peptidoglycan biosynthesis is regulated by the serine/threonine protein kinase PknA in Corynebacterium glutamicum
Q35539754The N-acetyl-D-glucosamine repressor NagC of Vibrio fischeri facilitates colonization of Euprymna scolopes.
Q33367782The PagN protein of Salmonella enterica serovar Typhimurium is an adhesin and invasin.
Q59807740The Phosphohistidine Phosphatase SixA Targets a Phosphotransferase System
Q46210806The Promiscuous sumA Missense Suppressor from Salmonella enterica Has an Intriguing Mechanism of Action
Q44881231The Pseudomonas syringae genome encodes a combined mannuronan C-5-epimerase and O-acetylhydrolase, which strongly enhances the predicted gel-forming properties of alginates.
Q42137312The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes
Q39934481The Streptomyces glaucescens TcmR protein represses transcription of the divergently oriented tcmR and tcmA genes by binding to an intergenic operator region
Q27655136The Structure of the Bacterial Oxidoreductase Enzyme DsbA in Complex with a Peptide Reveals a Basis for Substrate Specificity in the Catalytic Cycle of DsbA Enzymes
Q42369901The Topology of the l-Arginine Exporter ArgO Conforms to an Nin-Cout Configuration in Escherichia coli: Requirement for the Cytoplasmic N-Terminal Domain, Functional Helical Interactions, and an Aspartate Pair for ArgO Function
Q36888554The Yersiniabactin-Associated ATP Binding Cassette Proteins YbtP and YbtQ Enhance Escherichia coli Fitness during High-Titer Cystitis
Q33904519The amino-terminal domain of the prokaryotic enhancer-binding protein XylR is a specific intramolecular repressor
Q38323582The chbG gene of the chitobiose (chb) operon of Escherichia coli encodes a chitooligosaccharide deacetylase
Q54720967The cytotoxin of Pseudomonas aeruginosa: cytotoxicity requires proteolytic activation.
Q59201868The expression of recombinant genes from bacteriophage lambda strong promoters triggers the SOS response inEscherichia coli
Q35230528The frequency of translational misreading errors in E. coli is largely determined by tRNA competition.
Q73728631The frozen solution structure of p21 ras determined by ESEEM spectroscopy reveals weak coordination of Thr35 to the active site metal ion
Q34085725The hemK gene in Escherichia coli encodes the N(5)-glutamine methyltransferase that modifies peptide release factors
Q28211459The in vivo synthesis of plant sesquiterpenes by Escherichia coli
Q35603496The large ribosomal subunit protein L9 enables the growth of EF-P deficient cells and enhances small subunit maturation
Q39494600The level of expression of the minor pilin subunit, CooD, determines the number of CS1 pili assembled on the cell surface of Escherichia coli.
Q36150540The malX malY operon of Escherichia coli encodes a novel enzyme II of the phosphotransferase system recognizing glucose and maltose and an enzyme abolishing the endogenous induction of the maltose system
Q49121813The microinjected Drosophila melanogaster 1731 retrotransposon is activated after the midblastula stage of the amphibian Pleurodeles waltl development
Q42637285The mutT defect does not elevate chromosomal fragmentation in Escherichia coli because of the surprisingly low levels of MutM/MutY-recognized DNA modifications
Q34768162The non-pathogenic Escherichia coli strain W secretes SslE via the virulence-associated type II secretion system beta
Q28485969The novel sigma factor-like regulator RpoQ controls luminescence, chitinase activity, and motility in Vibrio fischeri
Q41672597The ornibactin biosynthesis and transport genes of Burkholderia cenocepacia are regulated by an extracytoplasmic function sigma factor which is a part of the Fur regulon
Q27653979The phage   major tail protein structure reveals a common evolution for long-tailed phages and the type VI bacterial secretion system
Q40799380The phosphocarrier protein HPr of Neisseria meningitidis interacts with the transcription regulator CrgA and its deletion affects capsule production, cell adhesion, and virulence
Q39963665The precursor tRNA 3'-CCA interaction with Escherichia coli RNase P RNA is essential for catalysis by RNase P in vivo
Q47244921The putative Arabidopsis homolog of yeast vps52p is required for pollen tube elongation, localizes to Golgi, and might be involved in vesicle trafficking
Q34172330The role of oxidoreductases in determining the function of the neisserial lipid A phosphoethanolamine transferase required for resistance to polymyxin.
Q54204804The role of temperature and bivalent ions in preparing competent Escherichia coli.
Q38323966The role of the trehalose system in regulating the maltose regulon of Escherichia coli
Q35073138The sodium/proline transporter PutP of Helicobacter pylori.
Q34851439The stress-responsive dgk gene from Streptococcus mutans encodes a putative undecaprenol kinase activity
Q24309493The thiol-specific antioxidant protein from human brain: gene cloning and analysis of conserved cysteine regions
Q35530976The transcript from the σ(28)-dependent promoter is translationally inert in the expression of the σ(28)-encoding gene fliA in the fliAZ operon of Salmonella enterica serovar Typhimurium
Q91529273The type VI secretion system protein AsaA in Acinetobacter baumannii is a periplasmic protein physically interacting with TssM and required for T6SS assembly
Q42443568Theophylline-dependent riboswitch as a novel genetic tool for strict regulation of protein expression in Cyanobacterium Synechococcus elongatus PCC 7942.
Q63359891Thermodynamics of nucleotide binding to NBS-I of the Bacillus subtilis preprotein translocase subunit SecA
Q44188679Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue
Q42457004Tight transcriptional control mechanism ensures stable high-level expression from T7 promoter-based expression plasmids
Q30300568TleA, a Tsh-like autotransporter identified in a human enterotoxigenic Escherichia coli strain
Q33901821Tn5AraOut mutagenesis for the identification of Yersinia pestis genes involved in resistance towards cationic antimicrobial peptides
Q39498918Towards single-copy gene expression systems making gene cloning physiologically relevant: lambda InCh, a simple Escherichia coli plasmid-chromosome shuttle system
Q38708051TraR directly regulates transcription initiation by mimicking the combined effects of the global regulators DksA and ppGpp
Q34624448Transcription regulatory elements are punctuation marks for DNA replication
Q33889419Transcription through a simple DNA repeat blocks replication elongation
Q38336788Transcriptional regulation of Alcaligenes eutrophus hydrogenase genes.
Q40535603Transcriptionally driven cruciform formation in vivo
Q43021494Transferring redox regulation properties from sorghum NADP-malate dehydrogenase to Thermus NAD-malate dehydrogenase
Q41534338Translation and fates of the gag protein of 1731, a Drosophila melanogaster retrotransposon
Q30159816Translocator proteins in the two-partner secretion family have multiple domains.
Q44552121Transmembrane domain II of the Na+/proline transporter PutP of Escherichia coli forms part of a conformationally flexible, cytoplasmic exposed aqueous cavity within the membrane
Q47273271Transmembrane region of bacterial chemoreceptor is capable of promoting protein clustering
Q34318538Transposition of cyanobacterium insertion element ISY100 in Escherichia coli
Q44732908Trigger factor peptidyl-prolyl cis/trans isomerase activity is not essential for the folding of cytosolic proteins in Escherichia coli
Q73839003Trinucleotide repeats affect DNA replication in vivo
Q37875026Tuning different expression parameters to achieve soluble recombinant proteins in E. coli: advantages of high-throughput screening.
Q27655760Two Structurally Independent Domains of E. coli NusG Create Regulatory Plasticity via Distinct Interactions with RNA Polymerase and Regulators
Q28829010Ubiquity of log-normal distributions in intra-cellular reaction dynamics
Q47824926Unravelling the complexity and redundancy of carbon catabolic repression in Pseudomonas fluorescens SBW25.
Q34013420Use of periplasmic target protein capture for phage display engineering of tight-binding protein-protein interactions
Q77702919Using chromosomal lacIQ1 to control expression of genes on high-copy-number plasmids in Escherichia coli
Q35162297Validation of a fluorescence-based screening concept to identify ribosome assembly defects in Escherichia coli
Q40872003Variable opacity (Opa) outer membrane proteins account for the cell tropisms displayed by Neisseria gonorrhoeae for human leukocytes and epithelial cells
Q40669088Vector promoters used in Klebsiella pneumoniae
Q42632087Widespread use of the glu-tRNAGln transamidation pathway among bacteria. A member of the alpha purple bacteria lacks glutaminyl-trna synthetase
Q89058875Wzx flippases exhibiting complex O-unit preferences require a new model for Wzx-substrate interactions
Q27665474X-Ray Structure and Site-Directed Mutagenesis Analysis of the Escherichia coli Colicin M Immunity Protein
Q40549367aroA-Deficient Salmonella enterica Serovar Typhimurium Is More Than a Metabolically Attenuated Mutant.
Q39567131cis-acting sequences required for NtcB-dependent, nitrite-responsive positive regulation of the nitrate assimilation operon in the cyanobacterium Synechococcus sp. strain PCC 7942
Q33460439lac repressor is an antivirulence factor of Salmonella enterica: its role in the evolution of virulence in Salmonella
Q39937611purU, a source of formate for purT-dependent phosphoribosyl-N-formylglycinamide synthesis

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