Apparent versus true gene expression changes of three hypoxia-related genes in autopsy derived tissue and the importance of normalisation

scientific article published on 30 October 2012

Apparent versus true gene expression changes of three hypoxia-related genes in autopsy derived tissue and the importance of normalisation is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1007/S00414-012-0787-2
P698PubMed publication ID23108458

P50authorMarielle VennemannQ48316924
P2093author name stringSabine Lutz-Bonengel
Tony Fracasso
Benedikt Vennemann
Antje Huth
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Housekeeping genes as internal standards: use and limitsQ29302686
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Quantitative cytokine gene expression in human tonsils at excision and during histoculture assessed by standardized and calibrated real-time PCR and novel data processingQ30885658
The effect of bisphosphonates on gene expression: GAPDH as a housekeeping or a new target gene?Q33235424
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Ribosomal 18S RNA prevails over glyceraldehyde-3-phosphate dehydrogenase and beta-actin genes as internal standard for quantitative comparison of mRNA levels in invasive and noninvasive human melanoma cell subpopulationsQ43690072
Validation of endogenous controls for gene expression studies in human adipocytes and preadipocytesQ43770853
Quantitative analysis of beta-actin, beta-2-microglobulin and porphobilinogen deaminase mRNA and their comparison as control transcripts for RT-PCR.Q43989560
Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies.Q44202854
Utility of the housekeeping genes 18S rRNA, beta-actin and glyceraldehyde-3-phosphate-dehydrogenase for normalization in real-time quantitative reverse transcriptase-polymerase chain reaction analysis of gene expression in human T lymphocytesQ44925185
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Tissue-specific differences in mRNA quantification of glucose transporter 1 and vascular endothelial growth factor with special regard to death investigations of fatal injuriesQ46775902
Quantitative RT-PCR assays of hypoxia-inducible factor-1alpha, erythropoietin and vascular endothelial growth factor mRNA transcripts in the kidneys with regard to the cause of death in medicolegal autopsyQ47848609
Direct comparison of GAPDH, beta-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxiaQ48189660
Stabilization of vascular endothelial growth factor mRNA by hypoxia-inducible factor 1.Q51726118
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RNA integrity in post-mortem samples: influencing parameters and implications on RT-qPCR assays.Q54372687
Real-time RT-PCR quantitative assays and postmortem degradation profiles of erythropoietin, vascular endothelial growth factor and hypoxia-inducible factor 1 alpha mRNA transcripts in forensic autopsy materials.Q54627588
Validation of housekeeping genes for normalizing RNA expression in real-time PCR.Q54708147
Hypoxia-inducible factor 1 levels vary exponentially over a physiologically relevant range of O2 tensionQ71738016
Forensic pathological investigation of myocardial hypoxia-inducible factor-1 alpha, erythropoietin and vascular endothelial growth factor in cardiac deathQ80795303
Effects of aging, dietary restriction and glucocorticoid treatment on housekeeping gene expression in rat cortex and hippocampus-evaluation by real time RT-PCRQ95823946
P433issue2
P921main subjectautopsyQ41482
hypoxiaQ105688
P304page(s)335-344
P577publication date2012-10-30
P1433published inInternational Journal of Legal MedicineQ1666665
P1476titleApparent versus true gene expression changes of three hypoxia-related genes in autopsy derived tissue and the importance of normalisation
P478volume127

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cites work (P2860)
Q39609515Decreased mRNA levels of cardiac Cx43 and ZO1 in sudden cardiac death related to coronary atherosclerosis: a pilot study
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