Abstract is: Nature Protocols, published by the Nature Publishing Group, is an on-line scientific journal publishing methods in a recipe-style format. The journal was launched in June 2006 and the content includes both classical methods and cutting-edge techniques relevant to the study of biological problems. The content on this site is divided into "Nature Protocols" and the "Protocol Exchange". Initially, all "Nature Protocols" were commissioned by editors, but it is now possible for authors to upload pre-submission enquiries. All Nature Protocols are peer reviewed, fully edited and styled prior to publication. New protocols are added to the site on a weekly basis.
| scientific journal | Q5633421 |
| P6981 | ACNP journal ID | 2401508 |
| P8375 | Crossref journal ID | 57048 |
| P1250 | Danish Bibliometric Research Indicator (BFI) SNO/CNO | 13380 |
| P1058 | ERA Journal ID | 39747 |
| P646 | Freebase ID | /m/0280g9j |
| P8903 | HAL journal ID | 17325 |
| P1160 | ISO 4 abbreviation | Nat. Protoc. |
| P236 | ISSN | 1750-2799 |
| 1754-2189 | ||
| P7363 | ISSN-L | 1750-2799 |
| P1277 | JUFO ID | 63777 |
| P1055 | NLM Unique ID | 101284307 |
| P856 | official website | http://www.nature.com/nprot/index.html |
| P10283 | OpenAlex ID | V109387254 |
| P3181 | OpenCitations bibliographic resource ID | 6698 |
| P7662 | Scilit journal ID | 1003497 |
| P1156 | Scopus source ID | 5800207366 |
| P4616 | UniProt journal ID | 3533 |
| P495 | country of origin | United Kingdom | Q145 |
| P1240 | Danish Bibliometric Research Indicator level | 1 | |
| 2 | |||
| P571 | inception | 2006-01-01 | |
| P8875 | indexed in bibliographic review | Scopus | Q371467 |
| Science Citation Index Expanded | Q104047209 | ||
| P407 | language of work or name | English | Q1860 |
| P123 | publisher | Nature Portfolio | Q180419 |
| P1476 | title | Nature Protocols |
| Q47339925 | 'Calling Cards' method for high-throughput identification of targets of yeast DNA-binding proteins. |
| Q45991488 | (13)C-based metabolic flux analysis. |
| Q47073979 | 13C-isotope-based protocol for prenyl lipid metabolic analysis in zebrafish embryos. |
| Q51955565 | 1H NMR metabolite fingerprinting and metabolomic analysis of perchloric acid extracts from plant tissues. |
| Q46809746 | 2-Nitroglycals: versatile intermediates for efficient and highly stereoselective base-catalyzed glycoside bond formations |
| Q48544510 | 220-plex microRNA expression profile of a single cell. |
| Q103836840 | 3D active stabilization for single-molecule imaging |
| Q30891498 | 3D computational reconstruction of tissues with hollow spherical morphologies using single-cell gene expression data |
| Q35145334 | 3D live fluorescence imaging of cellular dynamics using Bessel beam plane illumination microscopy |
| Q85016525 | 3D maps of RNA interhelical junctions |
| Q47269918 | 3D molecular cartography using LC-MS facilitated by Optimus and 'ili software. |
| Q48312602 | 3D mouse embryonic stem cell culture for generating inner ear organoids. |
| Q40366839 | 3D printing of versatile reactionware for chemical synthesis |
| Q46720230 | 3D spheroid model of mIMCD3 cells for studying ciliopathies and renal epithelial disorders |
| Q41979786 | 3DNA: a versatile, integrated software system for the analysis, rebuilding and visualization of three-dimensional nucleic-acid structures |
| Q56659004 | 3′ End cDNA amplification using classic RACE |
| Q80093315 | 5' end cDNA amplification using classic RACE |
| Q33889403 | 5' end-centered expression profiling using cap-analysis gene expression and next-generation sequencing |
| Q41579524 | A 12-step user guide for analyzing voxel-wise gray matter asymmetries in statistical parametric mapping (SPM). |
| Q35842312 | A 3D human neural cell culture system for modeling Alzheimer's disease |
| Q33645381 | A 96-well-plate-based optical method for the quantitative and qualitative evaluation of Pseudomonas aeruginosa biofilm formation and its application to susceptibility testing |
| Q31142763 | A Bayesian cluster analysis method for single-molecule localization microscopy data |
| Q83265675 | A C. elegans-based, whole animal, in vivo screen for the identification of antifungal compounds |
| Q36700567 | A LC-MS-based workflow for measurement of branched fatty acid esters of hydroxy fatty acids |
| Q40050360 | A PCR-based high-throughput screen with multiround sample pooling: application to somatic cell gene targeting |
| Q38416902 | A bacterial export system for generating extracellular amyloid aggregates |
| Q39673677 | A bimolecular affinity purification method under denaturing conditions for rapid isolation of a ubiquitinated protein for mass spectrometry analysis. |
| Q48234574 | A cell-free organelle-based in vitro system for studying the peroxisomal protein import machinery. |
| Q33571545 | A cell-free system for functional centromere and kinetochore assembly |
| Q48032985 | A cerebellar window for intravital imaging of normal and disease states in mice |
| Q90929577 | A cerebellopontine angle mouse model for the investigation of tumor biology, hearing, and neurological function in NF2-related vestibular schwannoma |
| Q115590279 | A checklist for assessing the methodological quality of concurrent tES-fMRI studies (ContES checklist): a consensus study and statement |
| Q118033397 | A chemo-mechanical cochleostomy preserves hearing for the in vivo functional imaging of cochlear cells |
| Q39845644 | A coculture assay to visualize and monitor interactions between migrating glioma cells and nerve fibers |
| Q42872336 | A coelenterazine-based luminescence assay to quantify high-molecular-weight superoxide anion scavenger activities |
| Q48181489 | A combined ex/in vivo assay to detect effects of exogenously added factors in neural stem cells |
| Q42155930 | A comparative cross-linking strategy to probe conformational changes in protein complexes |
| Q98784945 | A complete and flexible workflow for metaproteomics data analysis based on MetaProteomeAnalyzer and Prophane |
| Q97424712 | A complete pupillometry toolbox for real-time monitoring of locus coeruleus activity in rodents |
| Q38721732 | A complete workflow for high-resolution spectral-stitching nanoelectrospray direct-infusion mass-spectrometry-based metabolomics and lipidomics |
| Q34353170 | A complete workflow for the analysis of full-size ChIP-seq (and similar) data sets using peak-motifs. |
| Q40530891 | A composite critical-size rabbit mandibular defect for evaluation of craniofacial tissue regeneration |
| Q92503893 | A comprehensive guide to dynamic analysis of microbial gene expression using the 3D-printed PFIbox and a fluorescent reporter library |
| Q99207751 | A comprehensive guide to studying inflammasome activation and cell death |
| Q90288697 | A comprehensive pipeline for translational top-down proteomics from a single blood draw |
| Q58052580 | A computational framework to integrate high-throughput ‘-omics’ datasets for the identification of potential mechanistic links |
| Q51906053 | A computational method of prediction of the end-diastolic pressure-volume relationship by single beat. |
| Q51543538 | A computational pipeline for comparative ChIP-seq analyses. |
| Q42787013 | A concise and scalable route to L-azidohomoalanine |
| Q80285964 | A continuous fluorescence resonance energy transfer angiotensin I-converting enzyme assay |
| Q54443829 | A continuous method for the large-scale extraction of plasmid DNA by modified boiling lysis. |
| Q45875138 | A continuous process to extract plasmid DNA based on alkaline lysis. |
| Q33280771 | A convenient, high-throughput assay for measuring the relative cell permeability of synthetic compounds |
| Q46333561 | A convenient, high-throughput method for enzyme-luminescence detection of dopamine released from PC12 cells |
| Q58090255 | A cross-linking/mass spectrometry workflow based on MS-cleavable cross-linkers and the MeroX software for studying protein structures and protein-protein interactions |
| Q30855191 | A cryoinjury model in neonatal mice for cardiac translational and regeneration research |
| Q38943524 | A decade of progress in tissue engineering. |
| Q42007896 | A detergent-based assay for the detection of promiscuous inhibitors |
| Q33836710 | A differential proteome screening system for post-translational modification-dependent transcription factor interactions. |
| Q33280932 | A direct approach to quantification of the cellular uptake of cell-penetrating peptides using MALDI-TOF mass spectrometry |
| Q40150084 | A double-reporter splicing assay for determining splicing efficiency in mammalian cells |
| Q38267277 | A flow cytometry-based method to simplify the analysis and quantification of protein association to chromatin in mammalian cells |
| Q80805142 | A fluorescence-based double retrograde tracer strategy for charting central neuronal connections |
| Q50531166 | A fluorescence-based imaging method to measure in vitro and in vivo mitophagy using mt-Keima. |
| Q43030501 | A fluorescence-based in vitro assay for investigating early endosome dynamics |
| Q58477624 | A fluorescence-based protocol for quantifying angiotensin-converting enzyme activity |
| Q30741216 | A framework for parameter estimation and model selection from experimental data in systems biology using approximate Bayesian computation |
| Q92154269 | A gas chromatography-mass spectrometry-based whole-cell screening assay for target identification in distal cholesterol biosynthesis |
| Q33422847 | A general pipeline for quality and statistical assessment of protein interaction data using R and Bioconductor |
| Q41503587 | A general protocol for the crystallization of membrane proteins for X-ray structural investigation |
| Q34979957 | A general protocol for the generation of Nanobodies for structural biology |
| Q51984233 | A general purpose method for extracting RNA from Dictyostelium cells. |
| Q51918372 | A general user interface for prediction servers of proteins' post-translational modification sites. |
| Q90724242 | A general, electrocatalytic approach to the synthesis of vicinal diamines |
| Q100416125 | A generalized workflow for conducting electric field-optimized, fMRI-guided, transcranial magnetic stimulation |
| Q54404165 | A generic protocol for the expression and purification of recombinant RNA in Escherichia coli using a tRNA scaffold. |
| Q51032326 | A generic protocol for the expression and purification of recombinant proteins in Escherichia coli using a combinatorial His6-maltose binding protein fusion tag. |
| Q53683719 | A generic protocol for the purification and characterization of water-soluble complexes of affinity-tagged proteins and lipids. |
| Q50861893 | A gold nanoparticle-based chronocoulometric DNA sensor for amplified detection of DNA. |
| Q51556724 | A graphical and computational modeling platform for biological pathways. |
| Q97692974 | A graphical user interface to design high-throughput optogenetic experiments with the optoPlate-96 |
| Q35099191 | A guide to small-molecule structure assignment through computation of (¹H and ¹³C) NMR chemical shifts |
| Q46491859 | A guinea pig model of acute and chronic asthma using permanently instrumented and unrestrained animals |
| Q43199676 | A hearing and vestibular phenotyping pipeline to identify mouse mutants with hearing impairment |
| Q43030507 | A high-throughput assay of yeast cell lysis for drug discovery and genetic analysis. |
| Q33322719 | A high-throughput fluorescence-based glycosyltransferase screen and its application in directed evolution |
| Q46442514 | A high-throughput fluorimetric assay for angiotensin I-converting enzyme |
| Q39220822 | A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish |
| Q36726391 | A high-throughput in vivo micronucleus assay for genome instability screening in mice |
| Q48376771 | A high-throughput in vivo screening method in the mouse for identifying regulators of metastatic colonization. |
| Q42872346 | A high-throughput method for the quantitative analysis of auxins. |
| Q39873127 | A high-throughput splinkerette-PCR method for the isolation and sequencing of retroviral insertion sites |
| Q38750217 | A high-yield double-purification proteomics strategy for the identification of SUMO sites. |
| Q39575886 | A highly multiplexed and sensitive RNA-seq protocol for simultaneous analysis of host and pathogen transcriptomes |
| Q46321069 | A highly sensitive single-enzyme homocysteine assay |
| Q48273711 | A human intestinal M-cell-like model for investigating particle, antigen and microorganism translocation. |
| Q54964754 | A lectin microarray approach for the rapid analysis of bacterial glycans |
| Q33294342 | A light microscope-based double retrograde tracer strategy to chart central neuronal connections. |
| Q80422702 | A liquid chromatography-mass spectrometry method for the quantitative analysis of urinary endogenous estrogen metabolites |
| Q57804870 | A magnetic resonance tuning sensor for the MRI detection of biological targets |
| Q33305819 | A manual method for the purification of fluorescently labeled neurons from the mammalian brain |
| Q92669022 | A marker-independent lineage-tracing system to quantify clonal dynamics and stem cell functionality in cancer tissue |
| Q91106390 | A mass spectrometry workflow for measuring protein turnover rates in vivo |
| Q34056402 | A membrane capture assay for lipid kinase activity |
| Q33280936 | A method for C-terminal sequence analysis in the proteomic era (proteins cleaved with cyanogen bromide). |
| Q42872343 | A method for concurrent diazomethane synthesis and substrate methylation in a 96-sample format. |
| Q48214895 | A method for detergent-free isolation of membrane proteins in their local lipid environment. |
| Q40143374 | A method for genetic modification of human embryonic stem cells using electroporation |
| Q84071758 | A method for growing a biofilm under low shear at the air-liquid interface using the drip flow biofilm reactor |
| Q51989988 | A method for parental RNA interference in the wasp Nasonia vitripennis. |
| Q45966615 | A method for quantifying aggression in male Drosophila melanogaster. |
| Q80093271 | A method for the covalent capture and screening of diverse small molecules in a microarray format |
| Q80078291 | A method to enable the investigation of murine bronchial immune cells, their cytokines and mediators |
| Q51288044 | A method to resolve the composition of heterogeneous affinity-purified protein complexes assembled around a common protein by chemical cross-linking, gel electrophoresis and mass spectrometry. |
| Q43096004 | A methodology for the combined in situ analyses of the precursor and mature forms of microRNAs and correlation with their putative targets |
| Q80092912 | A microarray-based protocol for monitoring the growth of yeast overexpression strains |
| Q51110273 | A microfluidics-based method for measuring neuronal activity in Drosophila chemosensory neurons. |
| Q46896514 | A model of MPTP-induced Parkinson's disease in the goldfish |
| Q44870000 | A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide. |
| Q52680619 | A modified universal fast walking method for single-tube transposon mapping. |
| Q38717121 | A modular approach to the design, fabrication, and characterization of muscle-powered biological machines. |
| Q34735662 | A mouse model of intracerebral hemorrhage using autologous blood infusion |
| Q58320918 | A multispectral optical illumination system with precise spatiotemporal control for the manipulation of optogenetic reagents |
| Q34236010 | A murine model of urinary tract infection |
| Q42327908 | A mutagenesis and screening strategy to generate optimally thermostabilized membrane proteins for structural studies |
| Q36061347 | A nanoparticle formula for delivering siRNA or miRNAs to tumor cells in cell culture and in vivo. |
| Q92126192 | A nanostructure platform for live-cell manipulation of membrane curvature |
| Q92512568 | A non-enzymatic method for dissection of mouse bladder urothelial tissue |
| Q50699987 | A peroxidase-coupled continuous absorbance plate-reader assay for flavin monoamine oxidases, copper-containing amine oxidases and related enzymes. |
| Q37047996 | A pipeline for the generation of shRNA transgenic mice |
| Q86983159 | A plate-based electrochromic approach for the high-throughput detection of electrochemically active bacteria |
| Q36937203 | A positive/negative ion-switching, targeted mass spectrometry-based metabolomics platform for bodily fluids, cells, and fresh and fixed tissue |
| Q40548513 | A post-assembly genome-improvement toolkit (PAGIT) to obtain annotated genomes from contigs |
| Q58115622 | A practical guide to adaptive light-sheet microscopy |
| Q91666614 | A practical guide to intelligent image-activated cell sorting |
| Q91691721 | A practical guide to optimization in X10 expansion microscopy |
| Q38510457 | A practical guide to the MaxQuant computational platform for SILAC-based quantitative proteomics |
| Q33836704 | A practical guide to the synthesis and use of membrane-permeant acetoxymethyl esters of caged inositol polyphosphates. |
| Q41868144 | A practical guide to the synthesis of dinitroindolinyl-caged neurotransmitters |
| Q28296386 | A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC) |
| Q89723599 | A preclinical large-animal model for the assessment of critical-size load-bearing bone defect reconstruction |
| Q43880974 | A preclinical xenograft model of prostate cancer using human tumors |
| Q35229849 | A primary CD4(+) T cell model of HIV-1 latency established after activation through the T cell receptor and subsequent return to quiescence |
| Q51033611 | A primary culture technique of adult retina for regeneration studies on adult CNS neurons. |
| Q91229522 | A procedure for identifying possible products in the assembly-disassembly-organization-reassembly (ADOR) synthesis of zeolites |
| Q36779433 | A protocol describing pharynx counts and a review of other assays of apoptotic cell death in the nematode worm Caenorhabditis elegans |
| Q45857560 | A protocol describing the genetic correction of somatic human cells and subsequent generation of iPS cells. |
| Q43608114 | A protocol describing the principles of cis-regulatory analysis in the sea urchin |
| Q47735198 | A protocol describing the use of a recombinant protein-based, animal product-free medium (APEL) for human embryonic stem cell differentiation as spin embryoid bodies |
| Q80093367 | A protocol for Agrobacterium-mediated transformation in rice |
| Q28240928 | A protocol for Agrobacterium-mediated transformation of Brachypodium distachyon community standard line Bd21 |
| Q34615396 | A protocol for PAIR: PNA-assisted identification of RNA binding proteins in living cells |
| Q80093295 | A protocol for TILLING and Ecotilling in plants and animals |
| Q37177236 | A protocol for a lung neovascularization model in rodents |
| Q48195137 | A protocol for conducting 7-day daily renewal tests with Lemna gibba |
| Q50646033 | A protocol for constructing gene targeting vectors: generating knockout mice for the cadherin family and beyond. |
| Q48775303 | A protocol for culturing Drosophila melanogaster stage 9 egg chambers for live imaging. |
| Q81246968 | A protocol for designing siRNAs with high functionality and specificity |
| Q81201365 | A protocol for determination of anticardiolipin antibodies by ELISA |
| Q39792316 | A protocol for differential display of mRNA expression using either fluorescent or radioactive labeling |
| Q46580137 | A protocol for dissecting Drosophila melanogaster brains for live imaging or immunostaining |
| Q28240938 | A protocol for efficiently retrieving and characterizing flanking sequence tags (FSTs) in Brachypodium distachyon T-DNA insertional mutants |
| Q47794492 | A protocol for expression of foreign genes in chloroplasts |
| Q24603373 | A protocol for generating a high-quality genome-scale metabolic reconstruction |
| Q50665641 | A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice. |
| Q84817750 | A protocol for islet isolation from mouse pancreas |
| Q46791100 | A protocol for isolating Xenopus oocyte nuclear envelope for visualization and characterization by scanning electron microscopy (SEM) or transmission electron microscopy (TEM). |
| Q61638945 | A protocol for isolation and culture of human umbilical vein endothelial cells. |
| Q46170497 | A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow |
| Q83131551 | A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone |
| Q80805164 | A protocol for isolation and visualization of yeast nuclei by scanning electron microscopy (SEM) |
| Q34615443 | A protocol for mosaic analysis with a repressible cell marker (MARCM) in Drosophila |
| Q37177279 | A protocol for phenotypic detection and characterization of vascular cells of different origins in a lung neovascularization model in rodents |
| Q37201827 | A protocol for phenotypic detection and enumeration of circulating endothelial cells and circulating progenitor cells in human blood |
| Q33484159 | A protocol for preparing GFP-labeled neurons previously imaged in vivo and in slice preparations for light and electron microscopic analysis |
| Q33280973 | A protocol for preparing, characterizing and using three RNA-specific, live cell imaging probes: E36, E144 and F22. |
| Q40124490 | A protocol for rapid generation of recombinant adenoviruses using the AdEasy system |
| Q81997787 | A protocol for removal of antibiotic resistance cassettes from human embryonic stem cells genetically modified by homologous recombination or transgenesis |
| Q46140408 | A protocol for studying the kinetics of RNA within cultured cells: application to ribosomal RNA. |
| Q37208127 | A protocol for the culture and differentiation of highly polarized human retinal pigment epithelial cells |
| Q79845425 | A protocol for the in vitro selection of specific oligonucleotide probes for high-resolution DNA typing |
| Q30858585 | A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning |
| Q38773049 | A protocol for the systematic and quantitative measurement of protein-lipid interactions using the liposome-microarray-based assay |
| Q46443793 | A protocol for unraveling gene regulatory networks |
| Q34256839 | A quantitative high-throughput 96-well plate fluorescence assay for mechanism-based inactivators of cytochromes P450 exemplified using CYP2B6. |
| Q97636495 | A quantitative thiol reactivity profiling platform to analyze redox and electrophile reactive cysteine proteomes |
| Q46001943 | A radioactive uptake assay to measure ion transport across ion channel-containing liposomes. |
| Q46072595 | A rapid HPLC-MS/MS method for the simultaneous quantification of cyclosporine A, tacrolimus, sirolimus and everolimus in human blood samples |
| Q48876438 | A rapid Percoll gradient procedure for preparation of synaptosomes. |
| Q42844160 | A rapid and efficient route to benzazole heterocycles |
| Q37537077 | A rapid and reproducible assay for modeling myelination by oligodendrocytes using engineered nanofibers |
| Q50690799 | A rapid method for chloroplast isolation from the green alga Chlamydomonas reinhardtii. |
| Q34785155 | A rapid micro chromatin immunoprecipitation assay (microChIP). |
| Q39859960 | A rapid protocol for construction and production of high-capacity adenoviral vectors |
| Q42177676 | A rapid, comprehensive system for assaying DNA repair activity and cytotoxic effects of DNA-damaging reagents |
| Q48218870 | A rat model for neural circuitry abnormalities in schizophrenia |
| Q38758184 | A real-time fluorometric method for the simultaneous detection of cell death type and rate |
| Q34615464 | A real-time method of imaging glucose uptake in single, living mammalian cells. |
| Q51686329 | A reconstituted cell-free assay for the evaluation of the intrinsic activity of purified human ribosomes. |
| Q57002729 | A reliable general purpose method for extracting genomic DNA from Dictyostelium cells |
| Q84817754 | A reporter gene imaging system for monitoring microRNA biogenesis |
| Q47564825 | A reproducible and well-tolerated method for 2/3 partial hepatectomy in mice |
| Q87353467 | A ring barrier-based migration assay to assess cell migration in vitro |
| Q43677442 | A robust chromatin immunoprecipitation protocol for studying transcription factor-DNA interactions and histone modifications in wood-forming tissue |
| Q83379458 | A robust plant RNA isolation method suitable for Affymetrix GeneChip analysis and quantitative real-time RT-PCR |
| Q112080900 | A robust unsupervised machine-learning method to quantify the morphological heterogeneity of cells and nuclei |
| Q46295022 | A rodent model of myocardial infarction for testing the efficacy of cells and polymers for myocardial reconstruction |
| Q96610222 | A scalable SCENIC workflow for single-cell gene regulatory network analysis |
| Q57384141 | A scalable synthesis of 5,5′-dibromo-2,2′-bipyridine and its stepwise functionalization via Stille couplings |
| Q37292765 | A screening-based platform for the assessment of cellular respiration in Caenorhabditis elegans |
| Q33706960 | A semiautomated targeted sampling method to assess optic nerve axonal loss in a rat model of glaucoma |
| Q46236141 | A sensitive mass spectrometric method for hypothesis-driven detection of peptide post-translational modifications: multiple reaction monitoring-initiated detection and sequencing (MIDAS). |
| Q46140411 | A sensitive recombinant cell-based bioluminescent assay for detection of androgen-like compounds |
| Q39361963 | A simple and rapid nonviral approach to efficiently transfect primary tissue-derived cells using polyethylenimine |
| Q114866830 | A simple and reproducible 96-well plate-based method for the formation of fungal biofilms and its application to antifungal susceptibility testing |
| Q37340616 | A simple and reproducible 96-well plate-based method for the formation of fungal biofilms and its application to antifungal susceptibility testing. |
| Q36148286 | A simple approach for measuring FRET in fluorescent biosensors using two-photon microscopy |
| Q44875091 | A simple improved-throughput xylem protoplast system for studying wood formation. |
| Q30813018 | A simple method for imaging axonal transport in aging neurons using the adult Drosophila wing |
| Q80093394 | A simple method for quantitative determination of polysaccharides in fungal cell walls |
| Q31114516 | A simple one-pot multicomponent synthesis of an octahedral nanocontainer molecule |
| Q34104752 | A simple photoactivation and image analysis module for visualizing and analyzing axonal transport with high temporal resolution |
| Q46913494 | A simple slot blot for the detection of virtually all subtypes of the influenza A viral hemagglutinins using universal antibodies targeting the fusion peptide |
| Q97587574 | A simple sperm-sexing method that activates TLR7/8 on X sperm for the efficient production of sexed mouse or cattle embryos |
| Q34578351 | A simple trogocytosis-based method to detect, quantify, characterize and purify antigen-specific live lymphocytes by flow cytometry, via their capture of membrane fragments from antigen-presenting cells |
| Q39792323 | A simple, robust and semi-automated parasite egg isolation protocol |
| Q84261542 | A simple, versatile and efficient method to genetically modify human monocyte-derived dendritic cells with HIV-1-derived lentiviral vectors |
| Q44216453 | A single Drosophila embryo extract for the study of mitosis ex vivo |
| Q30643815 | A single vesicle-vesicle fusion assay for in vitro studies of SNAREs and accessory proteins |
| Q35561938 | A standardized protocol for repeated social defeat stress in mice. |
| Q99207743 | A standardized social preference protocol for measuring social deficits in mouse models of autism |
| Q46509993 | A strategy for co-translational folding studies of ribosome-bound nascent chain complexes using NMR spectroscopy |
| Q112288046 | A streamlined solution for processing, elucidating and quality control of cyclobutane pyrimidine dimer sequencing data |
| Q64082818 | A surgical orthotopic approach for studying the invasive progression of human bladder cancer |
| Q47200970 | A surgical orthotopic organoid transplantation approach in mice to visualize and study colorectal cancer progression |
| Q81803868 | A three-component Fischer indole synthesis |
| Q91322452 | A three-dimensional engineered heterogeneous tumor model for assessing cellular environment and response |
| Q35025905 | A three-stage biophysical screening cascade for fragment-based drug discovery. |
| Q39531429 | A time-of-drug addition approach to target identification of antiviral compounds |
| Q92503915 | A toolbox for the longitudinal assessment of healthspan in aging mice |
| Q34244995 | A transcription activator-like effector toolbox for genome engineering |
| Q36032518 | A transient parabiosis skin transplantation model in mice |
| Q48747424 | A two-component 'double-click' approach to peptide stapling. |
| Q98665199 | A universal approach for the synthesis of mesoporous gold, palladium and platinum films for applications in electrocatalysis |
| Q81577774 | A universal real-time PCR assay for the quantification of group-M HIV-1 proviral load |
| Q98952129 | A versatile reporter system for multiplexed screening of effective epigenome editors |
| Q89792530 | A whole-tissue RNA-seq toolkit for organism-wide studies of gene expression with PME-seq |
| Q92164086 | A workflow for generating multi-strain genome-scale metabolic models of prokaryotes |
| Q37085601 | AAV-mediated gene targeting methods for human cells |
| Q47911697 | ADP-ribose-specific chromatin-affinity purification for investigating genome-wide or locus-specific chromatin ADP-ribosylation |
| Q49052186 | AFLP technology for DNA fingerprinting. |
| Q49052158 | AFLP-based transcript profiling (cDNA-AFLP) for genome-wide expression analysis. |
| Q41496032 | ARQiv-HTS, a versatile whole-organism screening platform enabling in vivo drug discovery at high-throughput rates. |
| Q37078446 | Absolute quantification of protein and post-translational modification abundance with stable isotope-labeled synthetic peptides |
| Q37261547 | Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach |
| Q49092660 | Accelerated gas-liquid visible light photoredox catalysis with continuous-flow photochemical microreactors. |
| Q91322433 | Accessing crystal-crystal interaction forces with oriented nanocrystal atomic force microscopy probes |
| Q31108026 | Acquisition and voxelwise analysis of multi-subject diffusion data with tract-based spatial statistics |
| Q92611061 | Activation and expansion of human T cells using artificial antigen-presenting cell scaffolds |
| Q37069641 | Activation and measurement of free whisking in the lightly anesthetized rodent |
| Q48175463 | Active induction of experimental allergic encephalomyelitis |
| Q91705437 | Acute and rapid degradation of endogenous proteins by Trim-Away |
| Q34519522 | Adapting human pluripotent stem cells to high-throughput and high-content screening |
| Q92712494 | Addendum: A guide to small-molecule structure assignment through computation of (¹H and ¹³C) NMR chemical shifts |
| Q87978630 | Addendum: A reproducible and well-tolerated method for 2/3 partial hepatectomy in mice |
| Q92548327 | Addendum: Vibrational spectroscopic image analysis of biological material using multivariate curve resolution-alternating least squares (MCR-ALS) |
| Q35215169 | Addition of exogenous α-synuclein preformed fibrils to primary neuronal cultures to seed recruitment of endogenous α-synuclein to Lewy body and Lewy neurite-like aggregates. |
| Q57190003 | Adipose angiogenesis: quantitative methods to study microvessel growth, regression and remodeling in vivo |
| Q64357778 | Administration and interpretation of the Trail Making Test |
| Q50905189 | Adult mouse hematopoietic stem cells: purification and single-cell assays. |
| Q33896353 | Advanced CLARITY for rapid and high-resolution imaging of intact tissues |
| Q40453404 | Advanced CUBIC protocols for whole-brain and whole-body clearing and imaging. |
| Q91362897 | Advanced CUBIC tissue clearing for whole-organ cell profiling |
| Q36240152 | Advances in the field of single-particle cryo-electron microscopy over the last decade |
| Q43346151 | Advances in water treatment by adsorption technology |
| Q46290305 | Affinity purification of plasmid DNA by temperature-triggered precipitation |
| Q35096324 | Affinity purification-mass spectrometry and network analysis to understand protein-protein interactions |
| Q34750112 | Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances |
| Q51021816 | Agrobacterium rhizogenes-mediated transformation of soybean to study root biology. |
| Q34003305 | Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method |
| Q47913339 | Agrobacterium-mediated transformation of Brassica napus and Brassica oleracea. |
| Q59303686 | Agrobacterium-mediated transformation of friable embryogenic calli and regeneration of transgenic cassava |
| Q50973108 | Agrobacterium-mediated transformation of maize. |
| Q81201355 | Agrobacterium-mediated transformation of rice using immature embryos or calli induced from mature seed |
| Q47314494 | Agrobacterium-mediated transformation of the filamentous fungus Aspergillus awamori. |
| Q51032466 | Alignment and calibration of a focal neurotransmitter uncaging system. |
| Q43142484 | Alkyne-azide click reaction catalyzed by metallic copper under ultrasound. |
| Q29398642 | Ambient molecular imaging by desorption electrospray ionization mass spectrometry |
| Q80093408 | Amplification of 5' end cDNA with 'new RACE' |
| Q42033412 | An ELISA method to measure inhibition of the COX enzymes |
| Q46715836 | An ESI-MS method for characterization of native and modified oligonucleotides used for RNA interference and other biological applications |
| Q112742603 | An LC-MS/MS assay and complementary web-based tool to quantify and predict compound accumulation in E. coli |
| Q33305817 | An adapter ligation-mediated PCR method for high-throughput mapping of T-DNA inserts in the Arabidopsis genome. |
| Q114109148 | An analytical workflow for dynamic characterization and quantification of metal-bearing nanomaterials in biological matrices |
| Q82715944 | An antigen microarray immunoassay for multiplex screening of mouse monoclonal antibodies |
| Q56396191 | An approach to monitoring home-cage behavior in mice that facilitates data sharing |
| Q36090074 | An assay to probe Plasmodium falciparum growth, transmission stage formation and early gametocyte development |
| Q47294185 | An autonomous fueled machine that replicates catalytic nucleic acid templates for the amplified optical analysis of DNA. |
| Q33283986 | An efficient Mitsunobu protocol for the one-pot synthesis of S-glycosyl amino-acid building blocks and their use in combinatorial spot synthesis of glycopeptide libraries |
| Q39894986 | An efficient and easy-to-use cryopreservation protocol for human ES and iPS cells. |
| Q51572923 | An efficient chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in Arabidopsis plants. |
| Q57397526 | An efficient factor-depleted mammalian in vitro translation system |
| Q37715148 | An efficient method of directly cloning chimpanzee adenovirus as a vaccine vector |
| Q83146449 | An efficient petiole-feeding bioassay for introducing aqueous solutions into dicotyledonous plants |
| Q39859955 | An efficient platform for screening expression and crystallization of glycoproteins produced in human cells |
| Q42931689 | An environmental enrichment model for mice. |
| Q40150041 | An ex vivo method for rapid generation of monoclonal antibodies (ADLib system). |
| Q125199628 | An expanded CRISPR–Cas9-assisted recombineering toolkit for engineering genetically intractable Pseudomonas aeruginosa isolates |
| Q46896543 | An expedient, facile and simple one-pot synthesis of 2-methylenealkanoates and alkanenitriles from the Baylis-Hillman bromides in aqueous media |
| Q91458728 | An experimental murine model to study periodontitis |
| Q49985537 | An experimental toolbox for characterization of mammalian collagen type I in biological specimens. |
| Q80188331 | An improved synthesis of NHS-MAG3 for conjugation and radiolabeling of biomolecules with (99m)Tc at room temperature |
| Q48243869 | An improved toolbox to unravel the plant cellular machinery by tandem affinity purification of Arabidopsis protein complexes |
| Q34664017 | An inducible mouse model of colon carcinogenesis for the analysis of sporadic and inflammation-driven tumor progression |
| Q50622854 | An integrated pipeline for the multidimensional analysis of branching morphogenesis. |
| Q36032524 | An isolated tumor perfusion model in mice |
| Q53561783 | An isothermal primer extension method for whole genome amplification of fresh and degraded DNA: applications in comparative genomic hybridization, genotyping and mutation screening. |
| Q90591810 | An operant social self-administration and choice model in rats |
| Q102075535 | An optimized comet-based in vitro DNA repair assay to assess base and nucleotide excision repair activity |
| Q46502653 | An optimized method for measurement of gamma-H2AX in blood mononuclear and cultured cells |
| Q81577763 | An optimized procedure for whole-mount in situ hybridization on mouse embryos and embryoid bodies |
| Q40150009 | An optimized protocol for protein purification in cultured mammalian cells using a tandem affinity purification approach |
| Q30300853 | An optogenetics- and imaging-assisted simultaneous multiple patch-clamp recording system for decoding complex neural circuits |
| Q27310334 | An orthotopic mouse model of hepatocellular carcinoma with underlying liver cirrhosis |
| Q92483949 | An ultrasensitive photoelectrochemical platform for quantifying photoinduced electron-transfer properties of a single entity |
| Q48218851 | An ultrastructural readout of fluorescence recovery after photobleaching using correlative light and electron microscopy |
| Q48218694 | Analysis and interpretation of quadratic models of receptive fields |
| Q54190835 | Analysis of 3D gene expression patterns in plants using whole-mount RNA in situ hybridization. |
| Q34362782 | Analysis of GSH and GSSG after derivatization with N-ethylmaleimide |
| Q51839430 | Analysis of RhoA and Rho GEF activity in whole cells and the cell nucleus. |
| Q34615421 | Analysis of apoptosis by propidium iodide staining and flow cytometry |
| Q48702052 | Analysis of axonal growth and cell migration in 3D hydrogel cultures of embryonic mouse CNS tissue. |
| Q48138683 | Analysis of bacterial-surface-specific antibodies in body fluids using bacterial flow cytometry |
| Q112277268 | Analysis of combinatorial CRISPR screens with the Orthrus scoring pipeline |
| Q51574817 | Analysis of epigenetic modifications of chromatin at specific gene loci by native chromatin immunoprecipitation of nucleosomes isolated using hydroxyapatite chromatography. |
| Q53625004 | Analysis of glycosaminoglycan-derived, precolumn, 2-aminoacridone-labeled disaccharides with LC-fluorescence and LC-MS detection. |
| Q47630325 | Analysis of human cerebrospinal fluid monoamines and their cofactors by HPLC. |
| Q63953744 | Analysis of iron–sulfur protein maturation in eukaryotes |
| Q52678543 | Analysis of locomotor activity rhythms in Drosophila. |
| Q31097348 | Analysis of longitudinal data from animals with missing values using SPSS |
| Q46117450 | Analysis of methanotroph community composition using a pmoA-based microbial diagnostic microarray |
| Q87202128 | Analysis of microglial migration by a micropipette assay |
| Q33341058 | Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells |
| Q80093319 | Analysis of mitochondrial function using phosphorescent oxygen-sensitive probes |
| Q41814922 | Analysis of mutational spectra by denaturing capillary electrophoresis |
| Q84432755 | Analysis of neurotransmitter release mechanisms by photolysis of caged Ca²⁺ in an autaptic neuron culture system |
| Q34610638 | Analysis of nuclear RNA interference in human cells by subcellular fractionation and Argonaute loading |
| Q43105428 | Analysis of protein S-acylation by gas chromatography-coupled mass spectrometry using purified proteins |
| Q33288503 | Analysis of protein glycosylation by mass spectrometry |
| Q54277046 | Analysis of protein mixtures from whole-cell extracts by single-run nanoLC-MS/MS using ultralong gradients. |
| Q35177806 | Analysis of protein-ligand interactions by fluorescence polarization |
| Q91785780 | Analysis of redox landscapes and dynamics in living cells and in vivo using genetically encoded fluorescent sensors |
| Q41140286 | Analysis of resistance and tolerance to virus infection in Drosophila |
| Q52679615 | Analysis of synaptic transmission in Caenorhabditis elegans using an aldicarb-sensitivity assay. |
| Q34327699 | Analysis of synaptic vesicle endocytosis in synaptosomes by high-content screening. |
| Q96231001 | Analysis of task-based functional MRI data preprocessed with fMRIPrep |
| Q42037369 | Analysis of the kinetics of folding of proteins and peptides using circular dichroism |
| Q34222114 | Analysis of the proteolysis of bioactive peptides using a peptidomics approach. |
| Q40150031 | Analysis of translation initiation using a translation control reporter system |
| Q92453920 | Analysis pipelines for cancer genome sequencing in mice |
| Q33706971 | Analytical platform for metabolome analysis of microbial cells using methyl chloroformate derivatization followed by gas chromatography-mass spectrometry. |
| Q27887656 | Analyzing and interpreting genome data at the network level with ConsensusPathDB |
| Q91497031 | Analyzing bacterial extracellular vesicles in human body fluids by orthogonal biophysical separation and biochemical characterization |
| Q29999340 | Analyzing grooming microstructure in neurobehavioral experiments |
| Q27310381 | Analyzing membrane remodeling and fission using supported bilayers with excess membrane reservoir |
| Q33370529 | Analyzing multiple data sets by interconnecting RSAT programs via SOAP Web services: an example with ChIP-chip data |
| Q28131831 | Analyzing real-time PCR data by the comparative C(T) method |
| Q40267036 | Analyzing trapped protein complexes by Virotrap and SFINX. |
| Q47768748 | Ancient DNA extraction from bones and teeth |
| Q45005293 | Anesthetized- and awake-patched whole-cell recordings in freely moving rats using UV-cured collar-based electrode stabilization |
| Q80078265 | Anti-primer quenching-based real-time PCR for simplex or multiplex DNA quantification and single-nucleotide polymorphism genotyping |
| Q42213231 | Antibiotic resistance-mediated isolation of scaffold-specific natural product producers |
| Q47571305 | Antibody modeling using the prediction of immunoglobulin structure (PIGS) web server [corrected]. |
| Q54301393 | Antibody-based detection of protein phosphorylation status to track the efficacy of novel therapies using nanogram protein quantities from stem cells and cell lines. |
| Q38814625 | Antibody-coupled siRNA as an efficient method for in vivo mRNA knockdown |
| Q40262172 | Antifungal drug testing by combining minimal inhibitory concentration testing with target identification by gas chromatography-mass spectrometry. |
| Q103029572 | Antigen retrieval and clearing for whole-organ immunofluorescence by FLASH |
| Q42003336 | Antigen-specific activation and cytokine-facilitated expansion of naive, human CD8+ T cells |
| Q48406882 | Appetitive position discrimination in the T-maze. |
| Q64388834 | Application and optimization of CRISPR-Cas9-mediated genome engineering in axolotl (Ambystoma mexicanum) |
| Q45331582 | Application of a robustness screen for the evaluation of synthetic organic methodology |
| Q90526998 | Application of anisotropic NMR parameters to the confirmation of molecular structure |
| Q46350135 | Application of cationic conjugated polymers in microarrays using label-free DNA targets |
| Q33286528 | Application of direct current electric fields to cells and tissues in vitro and modulation of wound electric field in vivo |
| Q33280979 | Application of highly sensitive fluorescent dyes (CyDye DIGE Fluor saturation dyes) to laser microdissection and two-dimensional difference gel electrophoresis (2D-DIGE) for cancer proteomics |
| Q50688423 | Application of isothermal titration calorimetry and column chromatography for identification of biomolecular targets. |
| Q112331098 | Applications of and considerations for using CRISPR–Cas9-mediated gene conversion systems in rodents |
| Q51513968 | Applying label-free dynamic mass redistribution technology to frame signaling of G protein-coupled receptors noninvasively in living cells. |
| Q36240148 | Applying the INTACT method to purify endosperm nuclei and to generate parental-specific epigenome profiles |
| Q33954865 | Applying thiouracil tagging to mouse transcriptome analysis |
| Q80513396 | Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis |
| Q33515240 | Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays |
| Q58050184 | Array-MAPH: a methodology for the detection of locus copy-number changes in complex genomes |
| Q46182692 | Arrayed cellular microenvironments for identifying culture and differentiation conditions for stem, primary and rare cell populations |
| Q46664262 | Artificial restriction DNA cutter for site-selective scission of double-stranded DNA with tunable scission site and specificity |
| Q46850584 | Assay for apoptosis using the mitochondrial probes, Rhodamine123 and 10-N-nonyl acridine orange |
| Q80093433 | Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method |
| Q80092991 | Assay of protein kinases using radiolabeled ATP: a protocol |
| Q38303082 | Assaying RNA chaperone activity in vivo in bacteria using a ribozyme folding trap |
| Q46170501 | Assaying dynamic cell-cell junctional communication using noninvasive and quantitative fluorescence imaging techniques: LAMP and infrared-LAMP. |
| Q57385066 | Assaying proliferation and differentiation capacity of stem cells using disaggregated adult mouse epidermis |
| Q83366743 | Assaying stem cell mechanobiology on microfabricated elastomeric substrates with geometrically modulated rigidity |
| Q39792310 | Assays for in vitro monitoring of human airway smooth muscle (ASM) and human pulmonary arterial vascular smooth muscle (VSM) cell migration |
| Q80093387 | Assays for in vitro monitoring of proliferation of human airway smooth muscle (ASM) and human pulmonary arterial vascular smooth muscle (VSM) cells |
| Q36057839 | Assembling global maps of cellular function through integrative analysis of physical and genetic networks |
| Q94671869 | Assembly and operation of an open-source, computer numerical controlled (CNC) robot for performing cranial microsurgical procedures |
| Q27310304 | Assembly and operation of the autopatcher for automated intracellular neural recording in vivo. |
| Q51131813 | Assembly of multienzyme complexes on DNA nanostructures. |
| Q47174556 | Assembly of phospholipid nanodiscs of controlled size for structural studies of membrane proteins by NMR. |
| Q33733380 | Assessing corollary discharge in humans using noninvasive neurophysiological methods. |
| Q36245072 | Assessing fear and anxiety in humans using the threat of predictable and unpredictable aversive events (the NPU-threat test) |
| Q48218722 | Assessing hoarding in mice |
| Q95321769 | Assessing motor-related phenotypes of Caenorhabditis elegans with the wide field-of-view nematode tracking platform |
| Q48218833 | Assessing nest building in mice |
| Q52666970 | Assessing protein-ligand interaction scoring functions with the CASF-2013 benchmark. |
| Q47791885 | Assessing recent and remote associative olfactory memory in rats using the social transmission of food preference paradigm |
| Q90428274 | Assessing spatial pattern separation in rodents using the object pattern separation task |
| Q40182984 | Assessing the delivery efficacy and internalization route of cell-penetrating peptides |
| Q50882322 | Assessment of colon sensitivity by luminal distension in mice. |
| Q38817085 | Assessment of engineered cells using CellNet and RNA-seq. |
| Q52625967 | Assessment of mitochondrial respiratory chain enzymatic activities on tissues and cultured cells. |
| Q51573574 | Assessment of protein dynamics and DNA repair following generation of DNA double-strand breaks at defined genomic sites. |
| Q43247466 | Assessment of pyrogenic contaminations with validated human whole-blood assay |
| Q47314293 | Assessment of social transmission of threats in humans using observational fear conditioning |
| Q46941935 | Asymmetric aldol reaction using boron enolates |
| Q44221913 | Asymmetric synthesis of amines using tert-butanesulfinamide |
| Q92128771 | Asymmetric-flow field-flow fractionation technology for exomere and small extracellular vesicle separation and characterization |
| Q45774342 | Atomic force microscopy and chemical force microscopy of microbial cells |
| Q57825436 | Atomic force microscopy and spectroscopy of native membrane proteins |
| Q46333556 | Atomic force microscopy of supported lipid bilayers. |
| Q48444485 | Attachment of stem cells to scaffold particles for intra-cerebral transplantation. |
| Q90782122 | Author Correction: A practical guide to intelligent image-activated cell sorting |
| Q90591838 | Author Correction: Decellularization and antibody staining of mouse tissues to map native extracellular matrix structures in 3D |
| Q93042349 | Author Correction: Design, execution, and analysis of CRISPR-Cas9-based deletions and genetic interaction networks in the fungal pathogen Candida albicans |
| Q92432953 | Author Correction: Differentiation of cardiomyocytes and generation of human engineered heart tissue |
| Q90288689 | Author Correction: Efficient genetic engineering of human intestinal organoids using electroporation |
| Q89723603 | Author Correction: GRID-seq for comprehensive analysis of global RNA-chromatin interactions |
| Q57804873 | Author Correction: Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening |
| Q92095878 | Author Correction: Guidance for quantitative confocal microscopy |
| Q58105908 | Author Correction: Isolation and cultivation of naive-like human pluripotent stem cells based on HERVH expression |
| Q89475593 | Author Correction: SHERLOCK: nucleic acid detection with CRISPR nucleases |
| Q89242855 | Author Correction: Structural prediction of protein models using distance restraints derived from cross-linking mass spectrometry data |
| Q93042357 | Author Correction: Using the tube test to measure social hierarchy in mice |
| Q91199417 | Author Correction: V2a interneuron differentiation from mouse and human pluripotent stem cells |
| Q101133425 | Author Correction: lentiMPRA and MPRAflow for high-throughput functional characterization of gene regulatory elements |
| Q33291321 | Automated generation of a dihydropyrimidine compound library using microwave-assisted processing |
| Q46721300 | Automated identification of mouse visual areas with intrinsic signal imaging |
| Q27860936 | Automated macromolecular model building for X-ray crystallography using ARP/wARP version 7 |
| Q33732615 | Automated microfluidic protein immunoblotting |
| Q35960349 | Automated screening for small organic ligands using DNA-encoded chemical libraries. |
| Q30657369 | Automated selected reaction monitoring data analysis workflow for large-scale targeted proteomic studies |
| Q33280949 | Automated serum peptide profiling |
| Q82433541 | Automated solid-phase microextraction and thin-film microextraction for high-throughput analysis of biological fluids and ligand-receptor binding studies |
| Q51900357 | Automated structure determination of proteins with the SAIL-FLYA NMR method. |
| Q80092789 | Azido ruthenium: a new photoreactive probe for calcium-binding proteins |
| Q80078284 | BAC 'landing' on chromosomes of Brachypodium distachyon for comparative genome alignment |
| Q30399696 | Backbone assignment of perdeuterated proteins by solid-state NMR using proton detection and ultrafast magic-angle spinning |
| Q98281084 | Bacterial mock communities as standards for reproducible cytometric microbiome analysis |
| Q82125146 | Bar-coded hydrogel microparticles for protein detection: synthesis, assay and scanning |
| Q57798908 | Barcoded solid-phase RNA capture for Spatial Transcriptomics profiling in mammalian tissue sections |
| Q33886781 | Base-pair-resolution genome-wide mapping of active RNA polymerases using precision nuclear run-on (PRO-seq). |
| Q28818604 | Base-resolution profiling of active DNA demethylation using MAB-seq and caMAB-seq |
| Q47693396 | Base-resolution stratification of cancer mutations using functional variomics. |
| Q24618859 | Basic statistical analysis in genetic case-control studies |
| Q35172789 | Bayesian deconvolution and quantification of metabolites in complex 1D NMR spectra using BATMAN. |
| Q48175447 | Behavioral testing and preliminary analysis of the hamster visual system. |
| Q46016560 | Bicelle samples for solid-state NMR of membrane proteins. |
| Q48318628 | Bilateral multielectrode neurophysiological recordings coupled to local pharmacology in awake songbirds. |
| Q90836397 | Bilateral murine tumor models for characterizing the response to immune checkpoint blockade |
| Q92432958 | Bio-additive-based screening: toward evaluation of the biocompatibility of chemical reactions |
| Q48578686 | Bio-orthogonal labeling as a tool to visualize and identify newly synthesized proteins in Caenorhabditis elegans. |
| Q80093364 | Biochemical assays for multiple activation states of protein kinase C |
| Q50989035 | Bioconjugated quantum dots for multiplexed and quantitative immunohistochemistry. |
| Q40150089 | Biolistic transfection of neuronal cultures using a hand-held gene gun. |
| Q40171373 | Biological and chemical strategies for exploring inter- and intra-kingdom communication mediated via bacterial volatile signals. |
| Q39986163 | Bioluminescence imaging of hollow fibers in living animals: its application in monitoring molecular pathways |
| Q80092854 | Bioluminescence resonance energy transfer (BRET) for the real-time detection of protein-protein interactions |
| Q92615461 | Bioluminescence resonance energy transfer-based imaging of protein-protein interactions in living cells |
| Q92564936 | Biomimetic smoking robot for in vitro inhalation exposure compatible with microfluidic organ chips |
| Q57016985 | Biomolecular screening of formylpeptide receptor ligands with a sensitive, quantitative, high-throughput flow cytometry platform |
| Q50679965 | Biosensor-based assays for PQS, HHQ and related 2-alkyl-4-quinolone quorum sensing signal molecules. |
| Q89376243 | Bis-triazolyl diguanosine derivatives as synthetic transmembrane ion channels |
| Q33280942 | Blind patch clamp recordings in embryonic and adult mammalian brain slices |
| Q58090261 | Blood-brain-barrier organoids for investigating the permeability of CNS therapeutics |
| Q28296330 | Blue native PAGE |
| Q53561791 | BrdU assay for neurogenesis in rodents. |
| Q46011125 | Buccal micronucleus cytome assay. |
| Q37003461 | Building a morbidostat: an automated continuous-culture device for studying bacterial drug resistance under dynamically sustained drug inhibition. |
| Q30891496 | Building high-quality assay libraries for targeted analysis of SWATH MS data |
| Q51798510 | Burrowing in rodents: a sensitive method for detecting behavioral dysfunction. |
| Q51156538 | Butelase-mediated cyclization and ligation of peptides and proteins. |
| Q33280935 | COBRA: combined binary ratio labeling of nucleic-acid probes for multi-color fluorescence in situ hybridization karyotyping |
| Q112712280 | CODEX multiplexed tissue imaging with DNA-conjugated antibodies |
| Q96577267 | COVIDep: a web-based platform for real-time reporting of vaccine target recommendations for SARS-CoV-2 |
| Q34378409 | CRISPR interference (CRISPRi) for sequence-specific control of gene expression |
| Q92095869 | CRISPR off-target detection with DISCOVER-seq |
| Q90596404 | CRISPR-Cas9 genome engineering of primary CD4+ T cells for the interrogation of HIV-host factor interactions |
| Q97531625 | CRISPR-Cas9, CRISPRi and CRISPR-BEST-mediated genetic manipulation in streptomycetes |
| Q48261599 | CRISPR-Cas9-based genome-wide screening of Toxoplasma gondii. |
| Q58617578 | CRISPR-Cas9-mediated genome editing in apple and grapevine |
| Q99595130 | CRISPR-based engineering of gene knockout cells by homology-directed insertion in polyploid Drosophila S2R+ cells |
| Q52423173 | CRISPR/Cas-mediated genome editing in the rat via direct injection of one-cell embryos. |
| Q48166206 | CRISPR/Cas9 genome editing in human hematopoietic stem cells |
| Q35418660 | CRISPR/Cas9-mediated conversion of eGFP- into Gal4-transgenic lines in zebrafish. |
| Q33366972 | Calculating absolute and relative protein abundance from mass spectrometry-based protein expression data |
| Q33381531 | Calibrating excitation light fluxes for quantitative light microscopy in cell biology. |
| Q47162415 | Cancer imaging using surface-enhanced resonance Raman scattering nanoparticles |
| Q51032416 | Capping of CdSe-ZnS quantum dots with DHLA and subsequent conjugation with proteins. |
| Q36224514 | Capture and sequencing of NAD-capped RNA sequences with NAD captureSeq |
| Q48756076 | Capture of activity-induced ultrastructural changes at synapses by high-pressure freezing of brain tissue. |
| Q114554460 | Capture of the newly transcribed RNA interactome using click chemistry |
| Q93012484 | Capturing 5' and 3' native ends of mRNAs concurrently with Akron sequencing |
| Q46932119 | Capturing and cultivating single bacterial cells in gel microdroplets to obtain near-complete genomes |
| Q50893418 | Capturing suboptical dynamic structures in lipid bilayer patches formed from free-standing giant unilamellar vesicles. |
| Q80093199 | Carbon fiber technique for the investigation of single-cell mechanics in intact cardiac myocytes |
| Q90168557 | Carbon nanotube-mediated DNA delivery without transgene integration in intact plants |
| Q47363769 | Cardiac commitment of primate embryonic stem cells |
| Q48146853 | Cardiac differentiation of human pluripotent stem cells in scalable suspension culture |
| Q37391209 | Cardiac tissue engineering using perfusion bioreactor systems |
| Q35826313 | Catalytic C-H bond silylation of aromatic heterocycles |
| Q46333554 | Catalytic alkynone generation by Sonogashira reaction and its application in three-component pyrimidine synthesis. |
| Q36112726 | Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes. |
| Q39465667 | Cell analysis using a multiple internal reflection photonic lab-on-a-chip |
| Q40150021 | Cell culture and infection system for hepatitis C virus |
| Q38915258 | Cell cycle staging of individual cells by fluorescence microscopy. |
| Q39995043 | Cell cycle synchronization of animal cells and nuclei by centrifugal elutriation |
| Q40150014 | Cell cycle synchronization of tobacco BY-2 cells |
| Q42844157 | Cell separation using cryogel-based affinity chromatography. |
| Q33280951 | Cell tracking using a photoconvertible fluorescent protein |
| Q34249012 | Cell type-specific chromatin immunoprecipitation from multicellular complex samples using BiTS-ChIP. |
| Q33915619 | Cell type-specific mRNA purification by translating ribosome affinity purification (TRAP). |
| Q28268738 | Cell-PLoc: a package of Web servers for predicting subcellular localization of proteins in various organisms |
| Q47630351 | Cell-derived matrices for studying cell proliferation and directional migration in a complex 3D microenvironment |
| Q90894098 | Cell-type-specific metabolic labeling, detection and identification of nascent proteomes in vivo |
| Q36094990 | Cell-type-specific profiling of protein-DNA interactions without cell isolation using targeted DamID with next-generation sequencing |
| Q90894075 | Cell-type-specific quantification of protein synthesis in vivo |
| Q89901100 | CellPhoneDB: inferring cell-cell communication from combined expression of multi-subunit ligand-receptor complexes |
| Q89687029 | CellTagging: combinatorial indexing to simultaneously map lineage and identity at single-cell resolution |
| Q93271193 | Cellular thermal shift assay for the identification of drug-target interactions in the Plasmodium falciparum proteome |
| Q53121518 | Cellular, subcellular and functional in vivo labeling of the spinal cord using vital dyes. |
| Q80093153 | Cerasome as an infusible and cell-friendly gene carrier: synthesis of cerasome-forming lipids and transfection using cerasome |
| Q80092977 | Cervical duct cannulation in sheep for collection of afferent lymph dendritic cells from head tissues |
| Q51580771 | ChIP-on-chip protocol for genome-wide analysis of transcription factor binding in Drosophila melanogaster embryos. |
| Q40081554 | Changing medium and passaging cell lines |
| Q35145330 | Characterization of ancient and modern genomes by SNP detection and phylogenomic and metagenomic analysis using PALEOMIX. |
| Q33884375 | Characterization of bacterial spore germination using phase-contrast and fluorescence microscopy, Raman spectroscopy and optical tweezers |
| Q48125124 | Characterization of homodimer interfaces with cross-linking mass spectrometry and isotopically labeled proteins. |
| Q45345135 | Characterization of pluripotent stem cells |
| Q39753090 | Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells |
| Q33787941 | Characterization of the prime and non-prime active site specificities of proteases by proteome-derived peptide libraries and tandem mass spectrometry. |
| Q53056707 | Characterizing metal-binding sites in proteins with X-ray crystallography. |
| Q33294345 | Characterizing peptides in individual mammalian cells using mass spectrometry |
| Q36447330 | Characterizing the relative orientation and dynamics of RNA A-form helices using NMR residual dipolar couplings |
| Q28602619 | Characterizing the spatiotemporal expression of RNAs and proteins in the starlet sea anemone, Nematostella vectensis |
| Q87373925 | Chemical ablation of tumor-initiating human pluripotent stem cells |
| Q53561770 | Chemical cleavage of mismatch (CCM) to locate base mismatches in heteroduplex DNA. |
| Q30370270 | Chemical derivatization of histones for facilitated analysis by mass spectrometry. |
| Q33501994 | Chemical genetic screening in the zebrafish embryo |
| Q47347813 | Chemical synthesis of membrane proteins by the removable backbone modification method |
| Q53671255 | Chemical synthesis of proteins using peptide hydrazides as thioester surrogates. |
| Q42741470 | Chemical toxicity testing in vitro using cytochrome P450-expressing cell lines, such as human CYP1B1. |
| Q92669570 | Chemically defined and xenogeneic-free culture method for human epidermal keratinocytes on laminin-based matrices |
| Q50966061 | Chemically induced mouse models of acute and chronic intestinal inflammation. |
| Q42510602 | Chemically induced mouse models of intestinal inflammation |
| Q47874093 | Chemoenzymatic synthesis of glycoengineered IgG antibodies and glycosite-specific antibody-drug conjugates. |
| Q51686334 | Chemoenzymatic synthesis of the bacterial polysaccharide repeating unit undecaprenyl pyrophosphate and its analogs. |
| Q42126044 | Chiral sulfinamidourea and strong Brønsted acid-cocatalyzed enantioselective Povarov reaction to access tetrahydroquinolines |
| Q42008905 | Chromatin enrichment for proteomics |
| Q37189473 | Chromatin immunoprecipitation (ChIP) coupled to detection by quantitative real-time PCR to study transcription factor binding to DNA in Caenorhabditis elegans. |
| Q38346030 | Chromatin immunoprecipitation (ChIP) of plant transcription factors followed by sequencing (ChIP-SEQ) or hybridization to whole genome arrays (ChIP-CHIP). |
| Q34074262 | Chromatin immunoprecipitation and high-throughput sequencing from paraffin-embedded pathology tissue |
| Q34003310 | Chromatin immunoprecipitation and microarray-based analysis of protein location |
| Q98513736 | Chromatin integration labeling for mapping DNA-binding proteins and modifications with low input |
| Q33760102 | Chromatin tandem affinity purification sequencing. |
| Q112703050 | Chromatin tracing and multiplexed imaging of nucleome architectures (MINA) and RNAs in single mammalian cells and tissue |
| Q46563937 | Chromatin-state discovery and genome annotation with ChromHMM. |
| Q41855885 | Chromatographic assays of drug oxidation by human cytochrome P450 3A4 |
| Q50690849 | Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed. |
| Q34123747 | Chromosome orientation fluorescence in situ hybridization to study sister chromatid segregation in vivo |
| Q33600971 | Chromosome transfer in mature oocytes |
| Q112689166 | Chronic, cortex-wide imaging of specific cell populations during behavior |
| Q80198694 | Circular dichroism to determine binding mode and affinity of ligand-DNA interactions |
| Q33811695 | Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries |
| Q38301160 | Cis-regulatory hairpin-shaped mRNA encoding a reporter protein: catalytic sensing of nucleic acid sequence at single nucleotide resolution. |
| Q36126306 | Citrobacter rodentium mouse model of bacterial infection |
| Q58376389 | Click chemistry for targeted protein ubiquitylation and ubiquitin chain formation |
| Q34615388 | Clinical and empirical applications of the Rey-Osterrieth Complex Figure Test |
| Q90053840 | Clonal tracking using embedded viral barcoding and high-throughput sequencing |
| Q90836371 | Cloning of ground-state intestinal stem cells from endoscopic biopsy samples |
| Q54443848 | Cloning strategy, production and purification of proteins with exopeptidase-cleavable His-tags. |
| Q34615431 | Clonogenic assay of cells in vitro. |
| Q30576206 | Closed-loop optogenetic intervention in mice |
| Q50275781 | Cobalt-based nanocatalysts for green oxidation and hydrogenation processes. |
| Q91971532 | Cobaltaelectro-catalyzed C-H activation for resource-economical molecular syntheses |
| Q114872340 | Cognitive analysis of metabolomics data for systems biology |
| Q83554534 | Cold-induced activation of brown adipose tissue and adipose angiogenesis in mice |
| Q80093305 | Collagen antibody-induced arthritis |
| Q80422777 | Collagen-induced arthritis |
| Q86582412 | Collecting, rearing, spawning and inducing regeneration of the starlet sea anemone, Nematostella vectensis |
| Q53574020 | Collection of lymph-borne dendritic cells in the rat. |
| Q61832136 | Collection, pre-processing and on-the-fly analysis of data for high-resolution, single-particle cryo-electron microscopy |
| Q50639755 | Colocalization of fluorescent markers in confocal microscope images of plant cells. |
| Q47558200 | Colonoscopy-based colorectal cancer modeling in mice with CRISPR-Cas9 genome editing and organoid transplantation. |
| Q33280933 | Colony filtration blotting for screening soluble expression in Escherichia coli |
| Q46832828 | Color-coded fluorescence imaging of tumor-host interactions |
| Q33404043 | Combinatorial libraries of peptide dendrimers: design, synthesis, on-bead high-throughput screening, bead decoding and characterization |
| Q90724254 | Combined expansion microscopy with structured illumination microscopy for analyzing protein complexes |
| Q98303596 | Combined proximity labeling and affinity purification-mass spectrometry workflow for mapping and visualizing protein interaction networks |
| Q99248837 | Combined whole-mount fluorescence in situ hybridization and antibody staining in zebrafish embryos and larvae |
| Q51800437 | Combining competition assays with genetic complementation strategies to dissect mouse embryonic stem cell self-renewal and pluripotency. |
| Q41923775 | Combining confocal and atomic force microscopy to quantify single-virus binding to mammalian cell surfaces. |
| Q59198349 | Combining fluorescence imaging with Hi-C to study 3D genome architecture of the same single cell |
| Q84387320 | Combining genetic diversity, informatics and metabolomics to facilitate annotation of plant gene function |
| Q41761900 | Combining patch-clamping of cells in brain slices with immunocytochemical labeling to define cell type and developmental stage. |
| Q50273269 | Comparative analysis of S-fatty acylation of gel-separated proteins by stable isotope-coded fatty acid transmethylation and mass spectrometry. |
| Q47421780 | Compartmentalized partnered replication for the directed evolution of genetic parts and circuits |
| Q50912906 | Competitive PCR for precise nucleic acid quantification. |
| Q56996517 | Competitive PCR-ELISA protocols for the quantitative and the standardized detection of viral genomes |
| Q30278896 | Complementary IMAC enrichment methods for HLA-associated phosphopeptide identification by mass spectrometry |
| Q35172786 | Complementary X-ray tomography techniques for histology-validated 3D imaging of soft and hard tissues using plaque-containing blood vessels as examples. |
| Q97636489 | Complex-centric proteome profiling by SEC-SWATH-MS for the parallel detection of hundreds of protein complexes |
| Q53097951 | Comprehensive analysis of mitochondrial permeability transition pore activity in living cells using fluorescence-imaging-based techniques. |
| Q38804979 | Comprehensive analysis of mouse retinal mononuclear phagocytes. |
| Q44615124 | Comprehensive cluster analysis with Transitivity Clustering |
| Q33776986 | Comprehensive measurement of respiratory activity in permeabilized cells using extracellular flux analysis |
| Q40220427 | Comprehensive multilevel in vivo and in vitro analysis of heart rate fluctuations in mice by ECG telemetry and electrophysiology. |
| Q26269840 | Comprehensive qPCR profiling of gene expression in single neuronal cells |
| Q97587575 | Comprehensive structural glycomic characterization of the glycocalyxes of cells and tissues |
| Q88488759 | Computational 3D genome modeling using Chrom3D |
| Q35989427 | Computational protein-ligand docking and virtual drug screening with the AutoDock suite |
| Q33470200 | Computational reconstruction of cell and tissue surfaces for modeling and data analysis |
| Q30490786 | Conditional mouse lung cancer models using adenoviral or lentiviral delivery of Cre recombinase |
| Q48308780 | Conditional reprogramming and long-term expansion of normal and tumor cells from human biospecimens. |
| Q28831144 | Conditionally controlling nuclear trafficking in yeast by chemical-induced protein dimerization |
| Q46136549 | Conditioned place preference behavior in zebrafish |
| Q60118794 | Configuration of electrical spinal cord stimulation through real-time processing of gait kinematics |
| Q34008451 | Confocal laser endomicroscopy and narrow-band imaging-aided endoscopy for in vivo imaging of colitis and colon cancer in mice. |
| Q43111522 | Conjugation and radiolabeling of monoclonal antibodies with zirconium-89 for PET imaging using the bifunctional chelate p-isothiocyanatobenzyl-desferrioxamine |
| Q80092995 | Conjugation of DOTA-like chelating agents to peptides and radiolabeling with trivalent metallic isotopes |
| Q80092844 | Conjugation of chelating agents to proteins and radiolabeling with trivalent metallic isotopes |
| Q47310367 | Constructing cellular niche properties by localized presentation of Wnt proteins on synthetic surfaces. |
| Q51022622 | Constructing droplet interface bilayers from the contact of aqueous droplets in oil. |
| Q33310281 | Construction and calibration of an optical trap on a fluorescence optical microscope. |
| Q33455423 | Construction and flow cytometric screening of targeted enzyme libraries |
| Q33291318 | Construction and screening of BAC libraries made from Brachypodium genomic DNA. |
| Q82793602 | Construction and testing of engineered zinc-finger proteins for sequence-specific modification of mtDNA |
| Q58925486 | Construction and use of a microfluidic dissection platform for long-term imaging of cellular processes in budding yeast |
| Q33280988 | Construction and use of spotted large-insert clone DNA microarrays for the detection of genomic copy number changes |
| Q34161455 | Construction of BIBAC and BAC libraries from a variety of organisms for advanced genomics research |
| Q33413039 | Construction of Parallel Analysis of RNA Ends (PARE) libraries for the study of cleaved miRNA targets and the RNA degradome |
| Q33536984 | Construction of a femtosecond laser microsurgery system |
| Q37170443 | Construction of a liposome dialyzer for the preparation of high-value, small-volume liposome formulations |
| Q38610341 | Construction of an aerolysin nanopore in a lipid bilayer for single-oligonucleotide analysis |
| Q42287029 | Construction of conditional analog-sensitive kinase alleles in the fission yeast Schizosaccharomyces pombe |
| Q40150114 | Construction of diverse adeno-associated viral libraries for directed evolution of enhanced gene delivery vehicles |
| Q82893029 | Construction of implantable optical fibers for long-term optogenetic manipulation of neural circuits |
| Q51032459 | Construction of marker-free transplastomic tobacco using the Cre-loxP site-specific recombination system. |
| Q33280929 | Construction of semi-randomized gene libraries with weighted oligonucleotide synthesis and PCR. |
| Q33286535 | Construction of soil environmental DNA cosmid libraries and screening for clones that produce biologically active small molecules |
| Q33961029 | Continuous-flow bioseparation using microfabricated anisotropic nanofluidic sieving structures. |
| Q84616312 | Continuous-flow reactor-based synthesis of carbohydrate and dihydrolipoic acid-capped quantum dots |
| Q80093399 | Contour-clamped homogeneous electric field electrophoresis of Staphylococcus aureus |
| Q50478846 | Control of gene expression using a red- and far-red light-responsive bi-stable toggle switch. |
| Q48266324 | Controlled Fab-arm exchange for the generation of stable bispecific IgG1. |
| Q83993751 | Controlling gene loss of function in newts with emphasis on lens regeneration |
| Q84432746 | Convergent synthesis of aminomethylene peptidomimetics |
| Q50554910 | Conversion of adult mouse unipotent germline stem cells into pluripotent stem cells. |
| Q51115269 | Coordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells. |
| Q41199468 | Copper(I)/TEMPO-catalyzed aerobic oxidation of primary alcohols to aldehydes with ambient air. |
| Q44668565 | Copper-catalyzed asymmetric conjugate addition of alkylzirconium reagents to cyclic enones to form quaternary centers |
| Q56687895 | Copy-number analysis and inference of subclonal populations in cancer genomes using Sclust |
| Q40159192 | Correlated confocal and super-resolution imaging by VividSTORM. |
| Q28223741 | Correlated fluorescence microscopy and cryo-electron tomography of virus-infected or transfected mammalian cells |
| Q50236242 | Corrigendum: Cobalt-based nanocatalysts for green oxidation and hydrogenation processes. |
| Q87366009 | Corrigendum: Genome-wide copy number analysis of single cells |
| Q54261601 | Corrigendum: Measuring mRNA copy number in individual Escherichia coli cells using single-molecule fluorescent in situ hybridization. |
| Q86016488 | Corrigendum: Primary culture of ovarian surface epithelial cells and ascites-derived ovarian cancer cells from patients |
| Q85232150 | Corrigendum: RNAi-based biosynthetic pathway screens to identify in vivo functions of non-nucleic acid-based metabolites such as lipids |
| Q42921757 | Corrigendum: Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen |
| Q53231430 | Corrigendum: Site-specific NMR mapping and time-resolved monitoring of serine and threonine phosphorylation in reconstituted kinase reactions and mammalian cell extracts. |
| Q87366011 | Corrigendum: The PURE system for the cell-free synthesis of membrane proteins |
| Q86994656 | Corrigendum: The isolation and culture of endothelial colony-forming cells from human and rat lungs |
| Q50943770 | Corrigendum: a standardized protocol for repeated social defeat stress in mice. |
| Q86998146 | Corrigendum: functional genomics platform for pooled screening and generation of mammalian genetic interaction maps |
| Q53565684 | Corrigendum: solid-phase proximity ligation assays for individual or parallel protein analyses with readout via real-time PCR or sequencing. |
| Q86998142 | Corrigendum: using TRIP for genome-wide position effect analysis in cultured cells |
| Q52346330 | Cost-effective high-throughput single-haplotype iterative mapping and sequencing for complex genomic structures. |
| Q29577114 | Count-based differential expression analysis of RNA sequencing data using R and Bioconductor |
| Q48564988 | Coupling surface plasmon resonance to mass spectrometry to discover novel protein-protein interactions. |
| Q98513740 | Creating CRISPR-responsive smart materials for diagnostics and programmable cargo release |
| Q115732224 | Creating custom synthetic genomes in Escherichia coli with REXER and GENESIS |
| Q91842328 | Creating functional chromosome fusions in yeast with CRISPR-Cas9 |
| Q34181064 | Creating interactive, web-based and data-enriched maps with the Systems Biology Graphical Notation. |
| Q33811685 | Creating localized DNA double-strand breaks with microirradiation |
| Q45972997 | Creating self-illuminating quantum dot conjugates. |
| Q46799801 | Creating transgenic Drosophila by microinjecting the site-specific phiC31 integrase mRNA and a transgene-containing donor plasmid |
| Q91763183 | Creation and analysis of biochemical constraint-based models using the COBRA Toolbox v.3.0. |
| Q92154254 | Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD |
| Q46474062 | Creation of recombinant antigen-binding molecules derived from hybridomas secreting specific antibodies. |
| Q38463117 | Cross-species chromosome painting |
| Q35023140 | Cryo-EM of macromolecular assemblies at near-atomic resolution |
| Q48174115 | Cryoinjury as a myocardial infarction model for the study of cardiac regeneration in the zebrafish |
| Q39889546 | Cryopreservation and banking of mammalian cell lines |
| Q30835225 | Cryosectioning and immunolabeling |
| Q56674874 | Crystallization of soluble proteins in vapor diffusion for x-ray crystallography |
| Q29616431 | Crystallizing membrane proteins using lipidic mesophases |
| Q46941945 | Cu-catalyzed Goldberg and Ullmann reactions of aryl halides using chelating N- and O-based ligands |
| Q47988126 | Cu-catalyzed enantioselective allylic alkylation with organolithium reagents |
| Q47391226 | Cultivating previously uncultured soil bacteria using a soil substrate membrane system |
| Q47398290 | Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation |
| Q39995047 | Culture of Drosophila S2 cells and their use for RNAi-mediated loss-of-function studies and immunofluorescence microscopy |
| Q52699218 | Culture of Drosophila primary cells dissociated from gastrula embryos and their use in RNAi screening. |
| Q46553999 | Culture of embryonic-like stem cells from human umbilical cord blood and onward differentiation to neural cells in vitro |
| Q57273126 | Culture of human mast cells from peripheral blood progenitors |
| Q29615174 | Culturing hippocampal neurons |
| Q46753990 | Culturing pyramidal neurons from the early postnatal mouse hippocampus and cortex. |
| Q80093390 | Cyanogen bromide-activated coupling: DNA catalytic chromatography purification of EcoRI endonuclease |
| Q30360843 | Cysteine engineering of polyproteins for single-molecule force spectroscopy. |
| Q40150005 | Cytogenetic harvesting of commonly used tumor cell lines |
| Q53548886 | Cytokinesis-block micronucleus cytome assay. |
| Q34536289 | Cytometric fingerprinting for analyzing microbial intracommunity structure variation and identifying subcommunity function |
| Q110698450 | Cytosine and adenosine base editing in human pluripotent stem cells using transient reporters for editing enrichment |
| Q24657868 | DMS footprinting of structured RNAs and RNA-protein complexes |
| Q37369501 | DNA base mismatch detection with bulky rhodium intercalators: synthesis and applications |
| Q46312378 | DNA cleavage assay for the identification of topoisomerase I inhibitors |
| Q89687022 | DNA copy-number measurement of genome replication dynamics by high-throughput sequencing: the sort-seq, sync-seq and MFA-seq family |
| Q81247006 | DNA methylation analysis by pyrosequencing |
| Q61283947 | DNA methylation: Bisulphite modification and analysis |
| Q38316861 | DNA nanotubes for NMR structure determination of membrane proteins |
| Q35169621 | DNA origami-based standards for quantitative fluorescence microscopy. |
| Q38774619 | DNA sequencing technologies: 2006-2016. |
| Q51710181 | DNA stable-isotope probing. |
| Q31108031 | Data analysis of assorted serum peptidome profiles |
| Q52370989 | Data processing, multi-omic pathway mapping, and metabolite activity analysis using XCMS Online. |
| Q33749328 | Data quality control in genetic case-control association studies. |
| Q34582542 | De novo production of antigen-specific suppressor cells in vivo. |
| Q29615950 | De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis |
| Q91199412 | Decellularization and antibody staining of mouse tissues to map native extracellular matrix structures in 3D |
| Q47321614 | Decerebrate mouse model for studies of the spinal cord circuits. |
| Q87202121 | Deducing the mechanism of action of compounds identified in phenotypic screens by integrating their multiparametric profiles with a reference genetic screen |
| Q33909339 | Deep brain optical measurements of cell type-specific neural activity in behaving mice |
| Q33728879 | Deep interactome profiling of membrane proteins by co-interacting protein identification technology |
| Q111149752 | Deepometry, a framework for applying supervised and weakly supervised deep learning to imaging cytometry |
| Q57810989 | Defining CRISPR-Cas9 genome-wide nuclease activities with CIRCLE-seq |
| Q57829628 | Defining distance restraints in HADDOCK |
| Q36166032 | Defining human dendritic cell progenitors by multiparametric flow cytometry |
| Q57798898 | Defining informative priors for ensemble modeling in systems biology |
| Q38431799 | Defining transcribed regions using RNA-seq |
| Q80093396 | Dehydroalanine-containing peptides: preparation from phenylselenocysteine and utility in convergent ligation strategies |
| Q50690835 | Dendrimer-like DNA-based fluorescence nanobarcodes. |
| Q33280956 | Dendritic patch-clamp recording |
| Q50520993 | Depletion of hemoglobin and carbonic anhydrase from erythrocyte cytosolic samples by preparative clear native electrophoresis. |
| Q38724802 | Depletion of mitochondria in mammalian cells through enforced mitophagy. |
| Q51759823 | Depsipeptide substrates for sortase-mediated N-terminal protein ligation. |
| Q39673680 | Derivation and characterization of haploid embryonic stem cell cultures in medaka fish |
| Q33954806 | Derivation and characterization of mouse embryonic stem cells from permissive and nonpermissive strains |
| Q62086278 | Derivation and maintenance of human embryonic stem cells from poor-quality in vitro fertilization embryos |
| Q92503307 | Derivation and maintenance of mouse haploid embryonic stem cells |
| Q34313297 | Derivation and propagation of human embryonic stem cell lines from frozen embryos in an animal product-free environment. |
| Q92615456 | Derivation of enteric neuron lineages from human pluripotent stem cells |
| Q37590887 | Derivation of extraembryonic endoderm stem (XEN) cells from mouse embryos and embryonic stem cells. |
| Q39480684 | Derivation of genetically modified human pluripotent stem cells with integrated transgenes at unique mapped genomic sites |
| Q50669505 | Derivation of human embryonic stem cells from single blastomeres. |
| Q49140126 | Derivation of human embryonic stem cells using a post-inner cell mass intermediate. |
| Q63407375 | Derivation of mouse embryonic stem cells |
| Q51911549 | Derivation of neural crest cells from human pluripotent stem cells. |
| Q91303038 | Derivation of self-renewing lung alveolar epithelial type II cells from human pluripotent stem cells |
| Q46251265 | Deriving genotypes from RAD-seq short-read data using Stacks |
| Q52431346 | Design and assessment of engineered CRISPR-Cpf1 and its use for genome editing. |
| Q46412641 | Design and cloning of lentiviral vectors expressing small interfering RNAs |
| Q35238423 | Design and construction of a multiwavelength, micromirror total internal reflectance fluorescence microscope |
| Q38979281 | Design and construction of an in-plant activation cassette for transgene expression and recombinant protein production in plants. |
| Q80093493 | Design and construction of targeted AAVP vectors for mammalian cell transduction |
| Q36920080 | Design and delivery of antisense oligonucleotides to block microRNA function in cultured Drosophila and human cells. |
| Q33641074 | Design and generation of recombinant rabies virus vectors |
| Q33280958 | Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells |
| Q33298809 | Design and implementation of high-throughput RNAi screens in cultured Drosophila cells |
| Q39909447 | Design and implementation of in vivo imaging of neural injury responses in the adult Drosophila wing |
| Q91705444 | Design and operation of reconfigurable two-dimensional DNA molecular arrays |
| Q36401727 | Design and self-assembly of siRNA-functionalized RNA nanoparticles for use in automated nanomedicine |
| Q38313543 | Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli |
| Q37585784 | Design of a functional cyclic HSV1-TK reporter and its application to PET imaging of apoptosis |
| Q57798902 | Design of capillary microfluidics for spinning cell-laden microfibers |
| Q90069528 | Design, assembly, characterization, and operation of double-stranded interlocked DNA nanostructures |
| Q50445683 | Design, calibration and application of broad-range optical nanosensors for determining intracellular pH. |
| Q51032409 | Design, construction and in vitro testing of zinc finger nucleases. |
| Q91430086 | Design, execution, and analysis of CRISPR-Cas9-based deletions and genetic interaction networks in the fungal pathogen Candida albicans |
| Q97568985 | Design, fabrication and applications of tetrahedral DNA nanostructure-based multifunctional complexes in drug delivery and biomedical treatment |
| Q38783493 | Design, synthesis and evaluation of molecularly targeted hypoxia-activated prodrugs. |
| Q114109146 | Designing and executing prime editing experiments in mammalian cells |
| Q45733353 | Designing and optimizing comparative anchor primers for comparative gene mapping and phylogenetic inference |
| Q84942985 | Designing and using RNA scaffolds to assemble proteins in vivo |
| Q34018945 | Designing and using synthetic RNA thermometers for temperature-controlled gene expression in bacteria |
| Q34267117 | Designing candidate gene and genome-wide case-control association studies. |
| Q57666042 | Detailed mass analysis of structural heterogeneity in monoclonal antibodies using native mass spectrometry |
| Q35975147 | Detecting DNA double-stranded breaks in mammalian genomes by linear amplification-mediated high-throughput genome-wide translocation sequencing |
| Q43517595 | Detecting antigens by quantitative immuno-PCR. |
| Q35151839 | Detecting apoptosis of leukocytes in mouse lymph nodes |
| Q112730120 | Detecting chromosomal interactions in Capture Hi-C data with CHiCAGO and companion tools |
| Q33623329 | Detecting interactions with membrane proteins using a membrane two-hybrid assay in yeast |
| Q47794526 | Detecting ligands and dissecting nuclear receptor-signaling pathways using recombinant strains of the yeast Saccharomyces cerevisiae |
| Q38957867 | Detecting natural adaptation of the Streptococcus thermophilus CRISPR-Cas systems in research and classroom settings. |
| Q46850561 | Detecting protein-protein interactions by Far western blotting |
| Q30413211 | Detecting ultralow-frequency mutations by Duplex Sequencing |
| Q90284354 | Detection and quantification of RNA decay intermediates using XRN1-resistant reporter transcripts |
| Q33404046 | Detection and quantification of viable airborne bacteria and fungi using solid-phase cytometry |
| Q40025505 | Detection of 2-hydroxyethidium in cellular systems: a unique marker product of superoxide and hydroethidine. |
| Q54430200 | Detection of Mycoplasma in cell cultures. |
| Q112811820 | Detection of aberrant gene expression events in RNA sequencing data |
| Q52679613 | Detection of apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling and acridine orange in Drosophila embryos and adult male gonads. |
| Q28304911 | Detection of in vivo protein-DNA interactions using DamID in mammalian cells |
| Q46941943 | Detection of microRNAs in frozen tissue sections by fluorescence in situ hybridization using locked nucleic acid probes and tyramide signal amplification |
| Q35105300 | Detection of nascent RNA, single-copy DNA and protein localization by immunoFISH in mouse germ cells and preimplantation embryos |
| Q33280774 | Detection of non-covalent protein interactions by 'intensity fading' MALDI-TOF mass spectrometry: applications to proteases and protease inhibitors |
| Q38610348 | Detection of nucleic acid-protein interactions in plant leaves using fluorescence lifetime imaging microscopy |
| Q60304832 | Detection of protein SUMOylation in vivo |
| Q51794756 | Detection of protein-protein interactions using a simple survival protein-fragment complementation assay based on the enzyme dihydrofolate reductase. |
| Q50468322 | Detection of proteins using a colorimetric bio-barcode assay. |
| Q46421349 | Detection of two mRNA species at single-cell resolution by double-fluorescence in situ hybridization |
| Q57369673 | Detergent resistance as a tool in membrane research |
| Q40081558 | Determination of drug permeability and prediction of drug absorption in Caco-2 monolayers |
| Q37428132 | Determination of gene expression patterns using high-throughput RNA in situ hybridization to whole-mount Drosophila embryos |
| Q43221610 | Determination of gene expression patterns using in situ hybridization to Drosophila testes. |
| Q92483943 | Determination of hydroxyl groups in biorefinery resources via quantitative 31P NMR spectroscopy |
| Q51032384 | Determination of plant resistance to carbamate herbicidal compounds inhibiting cell division and early growth by seed and plantlets bioassays. |
| Q47650180 | Determination of site-specific glycan heterogeneity on glycoproteins |
| Q38829494 | Determination of the CYP1A-inducing potential of single substances, mixtures and extracts of samples in the micro-EROD assay with H4IIE cells |
| Q42067026 | Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism |
| Q46022821 | Determination of viral production in aquatic sediments using the dilution-based approach. |
| Q53067642 | Determining cell division symmetry through the dissection of dividing cells using single-cell expression analysis. |
| Q33280938 | Determining protein kinase substrate specificity by parallel solution-phase assay of large numbers of peptide substrates |
| Q34414349 | Determining proteome-wide expression levels using reverse protein arrays in fission yeast |
| Q33921230 | Determining the macropinocytic index of cells through a quantitative image-based assay |
| Q33999448 | Determining the number of specific proteins in cellular compartments by quantitative microscopy |
| Q60163693 | Determining the polysaccharide composition of plant cell walls |
| Q44703734 | Determining the role of specific signaling molecules during lymphocyte development in vivo: instant transgenesis |
| Q80093527 | Determining the stoichiometry and interactions of macromolecular assemblies from mass spectrometry |
| Q41015604 | Deubiquitinase-based analysis of ubiquitin chain architecture using Ubiquitin Chain Restriction (UbiCRest). |
| Q47797614 | Developing multiplexed SNP assays with special reference to degraded DNA templates. |
| Q33286532 | Development and optimization of high-throughput in vitro protein phosphatase screening assays |
| Q33600982 | Development of DNA aptamers using Cell-SELEX. |
| Q37235654 | Development of a highly reproducible three-dimensional organotypic model of the oral mucosa |
| Q96683715 | Development of a plasma pseudotargeted metabolomics method based on ultra-high-performance liquid chromatography-mass spectrometry |
| Q47670797 | Development of fertile mouse oocytes from mitotic germ cells in vitro |
| Q92503294 | Development, application and computational analysis of high-dimensional fluorescent antibody panels for single-cell flow cytometry |
| Q33361565 | Development, validation and implementation of immobilized metal affinity for phosphochemicals (IMAP)-based high-throughput screening assays for low-molecular-weight compound libraries |
| Q83162103 | Dictyostelium discoideum: a model host to measure bacterial virulence |
| Q52782412 | Differential centrifugation-based biochemical fractionation of the Drosophila adult CNS. |
| Q24633890 | Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks |
| Q90288706 | Differential viral accessibility (DIVA) identifies alterations in chromatin architecture through large-scale mapping of lentiviral integration sites |
| Q48061816 | Differentiation of cardiomyocytes and generation of human engineered heart tissue |
| Q37460841 | Differentiation of human oligodendrocytes from pluripotent stem cells |
| Q80093469 | Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines |
| Q40150131 | Differentiation of mouse embryonic stem cells to insulin-producing cells. |
| Q42993869 | Differentiation of rat multipotent adult progenitor cells to functional hepatocyte-like cells by mimicking embryonic liver development |
| Q50953421 | Differentiation of skeletal muscle and integration of myotubes with silicon microstructures using serum-free medium and a synthetic silane substrate. |
| Q37460845 | Differentiation of spinal motor neurons from pluripotent human stem cells |
| Q48156177 | Diffusion tensor imaging of the mouse brainstem and cervical spinal cord |
| Q53574041 | Digging and marble burying in mice: simple methods for in vivo identification of biological impacts. |
| Q38791131 | Digital PCR to assess gene-editing frequencies (GEF-dPCR) mediated by designer nucleases. |
| Q33294343 | Digital karyotyping. |
| Q40150036 | Diolistic labeling of neuronal cultures and intact tissue using a hand-held gene gun. |
| Q98303593 | Direct analysis of brain phenotypes via neural blastocyst complementation |
| Q92861036 | Direct and simultaneous observation of transcription and chromosome architecture in single cells with Hi-M |
| Q92669577 | Direct characterization of overproduced proteins by native mass spectrometry |
| Q36938504 | Direct conversion of human amniotic cells into endothelial cells without transitioning through a pluripotent state |
| Q48325835 | Direct conversion of mouse fibroblasts into induced neural stem cells. |
| Q122231183 | Direct delivery and fast-treated Agrobacterium co-culture (Fast-TrACC) plant transformation methods for Nicotiana benthamiana |
| Q57080195 | Direct detection of N−H⋯N hydrogen bonds in biomolecules by NMR spectroscopy |
| Q57080196 | Direct detection of N−H⋯O=C hydrogen bonds in biomolecules by NMR spectroscopy |
| Q80700346 | Direct detection of small RNAs using splinted ligation |
| Q50953439 | Direct ester condensation catalyzed by bulky diarylammonium pentafluorobenzenesulfonates. |
| Q91705461 | Direct fluorine-18 labeling of heat-sensitive biomolecules for positron emission tomography imaging using the Al18F-RESCA method |
| Q30492893 | Direct labeling and visualization of blood vessels with lipophilic carbocyanine dye DiI. |
| Q51820870 | Direct live monitoring of heterotypic axon-axon interactions in vitro. |
| Q35757379 | Direct metabolomics for plant cells by live single-cell mass spectrometry. |
| Q33947877 | Direct stochastic optical reconstruction microscopy with standard fluorescent probes |
| Q48395041 | Direct, long-term intrathecal application of therapeutics to the rodent CNS. |
| Q39464663 | Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions. |
| Q47784641 | Directed differentiation and long-term maintenance of epicardial cells derived from human pluripotent stem cells under fully defined conditions |
| Q50674541 | Directed differentiation of embryonic origin-specific vascular smooth muscle subtypes from human pluripotent stem cells. |
| Q33999627 | Directed differentiation of forebrain GABA interneurons from human pluripotent stem cells |
| Q35414493 | Directed differentiation of functional astroglial subtypes from human pluripotent stem cells |
| Q33787914 | Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells |
| Q89567134 | Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip |
| Q85839043 | Directed differentiation of human pluripotent cells to neural crest stem cells |
| Q39279039 | Directed differentiation of human pluripotent stem cells to cerebral cortex neurons and neural networks |
| Q40507104 | Directed evolution of artificial enzymes (XNAzymes) from diverse repertoires of synthetic genetic polymers |
| Q33536988 | Directed molecular evolution of DREADDs: a generic approach to creating next-generation RASSLs |
| Q47913320 | Directional conjugation of antibodies to nanoparticles for synthesis of multiplexed optical contrast agents with both delivery and targeting moieties. |
| Q42787021 | Disaccharide compositional analysis of heparan sulfate and heparin polysaccharides using UV or high-sensitivity fluorescence (BODIPY) detection. |
| Q57464842 | Discovery of potential causative mutations in human coding and noncoding genome with the interactive software BasePlayer |
| Q35066562 | Discovery of protein interactions using parallel analysis of translated ORFs (PLATO). |
| Q92949054 | Diselenide-selenoester ligation for chemical protein synthesis |
| Q38292056 | Displacement of protein-bound aptamers with small molecules screened by fluorescence polarization |
| Q39171014 | Disposable polymeric cryogel bioreactor matrix for therapeutic protein production |
| Q24610481 | Dissecting RNA folding by nucleotide analog interference mapping (NAIM) |
| Q35594443 | Dissecting protein reaction dynamics in living cells by fluorescence recovery after photobleaching. |
| Q36310356 | Dissection of mechanical force in living cells by super-resolved traction force microscopy |
| Q58414882 | Distinguishing cell types or populations based on the computational analysis of their infrared spectra |
| Q30252708 | Diverse protocols for correlative super-resolution fluorescence imaging and electron microscopy of chemically fixed samples |
| Q103029563 | Doubling the resolution of a confocal spinning-disk microscope using image scanning microscopy |
| Q44922640 | Drosophila melanogaster as a model host for studying Pseudomonas aeruginosa infection |
| Q48401978 | Drug-induced conditioned place preference and aversion in mice. |
| Q36766979 | Dual fluorescence detection of protein and RNA in Drosophila tissues. |
| Q33404041 | Dual fluorescence labeling of surface-exposed and internal proteins in erythrocytes infected with the malaria parasite Plasmodium falciparum |
| Q30636102 | Dual-view plane illumination microscopy for rapid and spatially isotropic imaging |
| Q35281804 | Dynamic clamp with StdpC software. |
| Q98394830 | Dynamic single-cell phenotyping of immune cells using the microfluidic platform DropMap |
| Q83993734 | Dynamic suspension culture for scalable expansion of undifferentiated human pluripotent stem cells |
| Q33379067 | E-clonal antibodies: selection of full-length IgG antibodies using bacterial periplasmic display |
| Q50574415 | EOS lentiviral vector selection system for human induced pluripotent stem cells. |
| Q47229150 | Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors. |
| Q35256020 | Ecto-Fc MS identifies ligand-receptor interactions through extracellular domain Fc fusion protein baits and shotgun proteomic analysis |
| Q59576133 | Efficient and irreversible antibody–cysteine bioconjugation using carbonylacrylic reagents |
| Q63407256 | Efficient and robust proteome-wide approaches for cross-linking mass spectrometry |
| Q40403124 | Efficient delivery of nuclease proteins for genome editing in human stem cells and primary cells |
| Q45876520 | Efficient derivation and inducible differentiation of expandable skeletal myogenic cells from human ES and patient-specific iPS cells. |
| Q41943614 | Efficient derivation of NPCs, spinal motor neurons and midbrain dopaminergic neurons from hESCs at 3% oxygen. |
| Q46246977 | Efficient derivation of functional dopaminergic neurons from human embryonic stem cells on a large scale |
| Q34083404 | Efficient expression of tyrosine-sulfated proteins in E. coli using an expanded genetic code |
| Q38976671 | Efficient gene delivery into cell lines and stem cells using baculovirus |
| Q50602652 | Efficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells. |
| Q38838399 | Efficient genetic engineering of human intestinal organoids using electroporation |
| Q45985116 | Efficient genome engineering approaches for the short-lived African turquoise killifish. |
| Q48218815 | Efficient imaging of amyloid deposits in Drosophila models of human amyloidoses |
| Q34273072 | Efficient introduction of specific TP53 mutations into mouse embryonic fibroblasts and embryonic stem cells |
| Q99350094 | Efficient low-cost chromatin profiling with CUT&Tag |
| Q88634349 | Efficient mouse genome engineering by CRISPR-EZ technology |
| Q31000174 | Efficient processing and analysis of large-scale light-sheet microscopy data |
| Q97531615 | Efficient recovery of the RNA-bound proteome and protein-bound transcriptome using phase separation (OOPS) |
| Q84387301 | Efficient siRNA-mediated prolonged gene silencing in human amniotic fluid stem cells |
| Q57253302 | Efficient transfection of DNA or shRNA vectors into neurons using magnetofection |
| Q39363166 | Efficient ΦC31 integrase-mediated site-specific germline transformation of Anopheles gambiae |
| Q38727158 | Efficient, footprint-free human iPSC genome editing by consolidation of Cas9/CRISPR and piggyBac technologies |
| Q30491543 | Electrical stimulation systems for cardiac tissue engineering. |
| Q53078889 | Electroactive poly(vinylidene fluoride)-based structures for advanced applications. |
| Q38764136 | Electrochemical detection of nucleic acids, proteins, small molecules and cells using a DNA-nanostructure-based universal biosensing platform. |
| Q41612675 | Electron cryotomography sample preparation using the Vitrobot |
| Q46196698 | Electron microscopy using the genetically encoded APEX2 tag in cultured mammalian cells |
| Q41902384 | Electrophoretic mobility shift assay (EMSA) for detecting protein-nucleic acid interactions |
| Q48129270 | Electrophysiological evaluation of engrafted stem cell-derived neurons |
| Q57959809 | Elucidating membrane structure and protein behavior using giant plasma membrane vesicles |
| Q42184793 | Embedding, serial sectioning and staining of zebrafish embryos using JB-4 resin |
| Q57691951 | Embryonic and induced pluripotent stem cell staining and sorting with the live-cell fluorescence imaging probe CDy1 |
| Q96641292 | Encapsulation and release of living tumor cells using hydrogels with the hybridization chain reaction |
| Q98513738 | Engineering DNA nanostructures for siRNA delivery in plants |
| Q112758358 | Engineering SARS-CoV-2 using a reverse genetic system |
| Q46410428 | Engineering a humanized bone organ model in mice to study bone metastases |
| Q35126438 | Engineering and characterizing monomeric fluorescent proteins for live-cell imaging applications. |
| Q35060703 | Engineering cells with intracellular agent-loaded microparticles to control cell phenotype |
| Q46776849 | Engineering complex biological systems in bacteria through recombinase-assisted genome engineering |
| Q46170507 | Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology |
| Q50849632 | Engineering genetic circuits that compute and remember. |
| Q93162829 | Engineering of human cardiac muscle electromechanically matured to an adult-like phenotype |
| Q89901103 | Engineering primary T cells with chimeric antigen receptors for rewired responses to soluble ligands |
| Q91931558 | Engineering proteins for allosteric control by light or ligands |
| Q91362890 | Engineering, delivery, and biological validation of artificial microRNA clusters for gene therapy applications |
| Q41943264 | Enhanced delivery of cell-penetrating peptide-peptide nucleic acid conjugates by endosomal disruption |
| Q27310315 | Enhancer scanning to locate regulatory regions in genomic loci |
| Q34142610 | Enriching libraries of high-aspect-ratio micro- or nanostructures by rapid, low-cost, benchtop nanofabrication |
| Q96131965 | Ensuring accurate resource identification |
| Q46299597 | Enzymatic assay for total plasma Cys. |
| Q84106669 | Enzymatic incorporation of an azide-modified UTP analog into oligoribonucleotides for post-transcriptional chemical functionalization |
| Q46140645 | Enzymatic ligation assisted by nucleases: simultaneous ligation and digestion promote the ordered assembly of DNA. |
| Q34041461 | Enzymatic route to preparative-scale synthesis of UDP-GlcNAc/GalNAc, their analogues and GDP-fucose |
| Q46421365 | Enzyme assay and activity fingerprinting of hydrolases with the red-chromogenic adrenaline test |
| Q53107806 | Enzyme-linked immunospot assays for direct ex vivo measurement of vaccine-induced human humoral immune responses in blood. |
| Q34398300 | Epitope-tagged protein-based artificial miRNA screens for optimized gene silencing in plants |
| Q58027784 | Erratum: Corrigendum: Bio-orthogonal labeling as a tool to visualize and identify newly synthesized proteins in Caenorhabditis elegans |
| Q59204075 | Erratum: Corrigendum: Efficient derivation and inducible differentiation of expandable skeletal myogenic cells from human ES and patient-specific iPS cells |
| Q63945799 | Erratum: Corrigendum: Gas chromatography mass spectrometry–based metabolite profiling in plants |
| Q87366006 | Erratum: Mapping Argonaute and conventional RNA-binding protein interactions with RNA at single-nucleotide resolution using HITS-CLIP and CIMS analysis |
| Q48667674 | Erratum: RGB marking with lentiviral vectors for multicolor clonal cell tracking. |
| Q47570567 | Erratum: antibody modeling using the Prediction of ImmunoGlobulin Structure (PIGS) web server |
| Q33280972 | Error-prone rolling circle amplification: the simplest random mutagenesis protocol |
| Q35041892 | Establishing a human renal cell carcinoma tumorgraft platform for preclinical drug testing |
| Q88721979 | Establishing anaerobic hydrocarbon-degrading enrichment cultures of microorganisms under strictly anoxic conditions |
| Q99248835 | Establishment and differentiation of long-term trophoblast organoid cultures from the human placenta |
| Q63681833 | Establishment of 3D organotypic cultures using human neonatal epidermal cells |
| Q103029566 | Establishment of human fetal hepatocyte organoids and CRISPR-Cas9-based gene knockin and knockout in organoid cultures from human liver |
| Q34003315 | Establishment of human tumor xenografts in immunodeficient mice |
| Q84862145 | Establishment of outgrowth endothelial cells from peripheral blood |
| Q99418586 | Establishment of patient-derived cancer organoids for drug-screening applications |
| Q35029148 | Establishment of primary cultures of human brain microvascular endothelial cells to provide an in vitro cellular model of the blood-brain barrier |
| Q46849426 | Estimation of baculovirus titer based on viable cell size |
| Q57014941 | Estimation of nuclear DNA content in plants using flow cytometry |
| Q80188305 | Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent |
| Q39943005 | Evaluating cell-surface expression and measuring activation of mammalian odorant receptors in heterologous cells |
| Q80093166 | Evaluating the biological relevance of putative enhancers using Tol2 transposon-mediated transgenesis in zebrafish |
| Q36441985 | Evaluation of bone regeneration using the rat critical size calvarial defect |
| Q33515241 | Evaluation of postnatal arteriogenesis and angiogenesis in a mouse model of hind-limb ischemia |
| Q38372293 | Evaluation of telomere length in human cardiac tissues using cardiac quantitative FISH. |
| Q30571358 | Evaluation of the pathogenesis and treatment of Mycobacterium marinum infection in zebrafish. |
| Q89486187 | Evoking and tracking zebrafish eye movement in multiple larvae with ZebEyeTrack |
| Q40150046 | Evolving proteins in mammalian cells using somatic hypermutation |
| Q92095841 | Ex situ determination of freely dissolved concentrations of hydrophobic organic chemicals in sediments and soils: basis for interpreting toxicity and assessing bioavailability, risks and remediation necessity |
| Q61365513 | Ex vivo T cell–based HIV suppression assay to evaluate HIV-specific CD8+ T-cell responses |
| Q91229510 | Ex vivo and in vivo stable isotope labelling of central carbon metabolism and related pathways with analysis by LC-MS/MS |
| Q36973746 | Ex vivo imaging of motor axon dynamics in murine triangularis sterni explants |
| Q37714085 | Ex vivo perfusion of mid-to-late-gestation mouse placenta for maternal-fetal interaction studies during pregnancy |
| Q53194386 | Ex vivo time-lapse confocal imaging of the mouse embryo aorta. |
| Q39814215 | Ex vivo tools for the clonal analysis of zebrafish hematopoiesis |
| Q34312041 | Exosome-mediated delivery of siRNA in vitro and in vivo |
| Q80093150 | Expanded-bed adsorption immobilized-metal affinity chromatography |
| Q34097128 | Expander: from expression microarrays to networks and functions |
| Q47273146 | Expansion of patient-derived circulating tumor cells from liquid biopsies using a CTC microfluidic culture device. |
| Q43172282 | Experimental acute biliary pancreatitis induced by retrograde infusion of bile acids into the mouse pancreatic duct |
| Q87933762 | Experimental determination of invasive fitness in Caenorhabditis elegans |
| Q44840828 | Explanatory signal interpretation and metabolite identification strategies for nominal mass FIE-MS metabolite fingerprints |
| Q30753580 | Explant culture of adult zebrafish hearts for epicardial regeneration studies |
| Q35085900 | Exploring protein-DNA interactions in 3D using in situ construction, manipulation and visualization of individual DNA dumbbells with optical traps, microfluidics and fluorescence microscopy |
| Q59765643 | Exponential scaling of single-cell RNA-seq in the past decade |
| Q83993755 | Expressing exogenous genes in newts by transgenesis |
| Q33404037 | Expression cloning and radiotracer uptakes in Xenopus laevis oocytes |
| Q33848897 | Expression microdissection adapted to commercial laser dissection instruments. |
| Q54342993 | Expression of high-affinity human antibody fragments in bacteria. |
| Q43281026 | Expression, purification and use of recombinant annexin V for the detection of apoptotic cells |
| Q42635737 | Extensible visualization and analysis for multidimensional images using Vaa3D. |
| Q27310390 | Extinction of conditioned opiate withdrawal in rats in a two-chambered place conditioning apparatus |
| Q36817556 | Extracellular protease digestion to evaluate membrane protein cell surface localization. |
| Q80093282 | Extraction and analysis of soluble inositol polyphosphates from yeast |
| Q57464844 | Extraction of highly degraded DNA from ancient bones, teeth and sediments for high-throughput sequencing |
| Q80422717 | Extraction, purification and analysis of histones |
| Q88132127 | FACS isolation of endothelial cells and pericytes from mouse brain microregions |
| Q34713970 | FACS purification of Drosophila larval neuroblasts for next-generation sequencing |
| Q41307733 | FISH-Flow, a protocol for the concurrent detection of mRNA and protein in single cells using fluorescence in situ hybridization and flow cytometry. |
| Q124852129 | FLEP-seq: simultaneous detection of RNA polymerase II position, splicing status, polyadenylation site and poly(A) tail length at genome-wide scale by single-molecule nascent RNA sequencing |
| Q83131540 | FLOTAC: new multivalent techniques for qualitative and quantitative copromicroscopic diagnosis of parasites in animals and humans |
| Q92611075 | FLOW-MAP: a graph-based, force-directed layout algorithm for trajectory mapping in single-cell time course datasets |
| Q44159967 | FLP/FRT-mediated conditional mutagenesis in pre-erythrocytic stages of Plasmodium berghei. |
| Q28307493 | FRET measurements of intracellular cAMP concentrations and cAMP analog permeability in intact cells |
| Q30577343 | Fabrication and application of flexible, multimodal light-emitting devices for wireless optogenetics |
| Q30541307 | Fabrication and operation of GRIN probes for in vivo fluorescence cellular imaging of internal organs in small animals |
| Q92565044 | Fabrication and practical applications of molybdenum disulfide nanopores |
| Q48304182 | Fabrication and utility of a transparent graphene neural electrode array for electrophysiology, in vivo imaging, and optogenetics. |
| Q46896535 | Fabrication of carbohydrate chips and their use to probe protein-carbohydrate interactions |
| Q51032349 | Fabrication of cell-containing gel modules to assemble modular tissue-engineered constructs [corrected]. |
| Q83823485 | Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro |
| Q48125745 | Fabrication of nanopores with ultrashort single-walled carbon nanotubes inserted in a lipid bilayer. |
| Q37442480 | Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells |
| Q51009454 | Fabrication of silicon nanowire devices for ultrasensitive, label-free, real-time detection of biological and chemical species. |
| Q39733521 | Fabrication of synthetic polymer coatings and their use in feeder-free culture of human embryonic stem cells. |
| Q93066031 | Facile 18F labeling of non-activated arenes via a spirocyclic iodonium(III) ylide method and its application in the synthesis of the mGluR5 PET radiopharmaceutical [18F]FPEB |
| Q62721634 | Facile synthesis of Ag nanocubes and Au nanocages |
| Q47577361 | Facile synthesis of gold nanomaterials with unusual crystal structures. |
| Q46664255 | Facile synthesis of mono-6-amino-6-deoxy-alpha-, beta-, gamma-cyclodextrin hydrochlorides for molecular recognition, chiral separation and drug delivery |
| Q67228145 | Facile synthesis to tune size, textural properties and fiber density of dendritic fibrous nanosilica for applications in catalysis and CO2 capture |
| Q93040369 | Facilitating chemical and biochemical experiments with electronic microcontrollers and single-board computers |
| Q50905819 | Fast and easy phosphopeptide fractionation by combinatorial ERLIC-SCX solid-phase extraction for in-depth phosphoproteome analysis. |
| Q93184608 | Fast and high-resolution mapping of elastic properties of biomolecules and polymers with bimodal AFM |
| Q41728621 | Fast sampling method for mammalian cell metabolic analyses using liquid chromatography-mass spectrometry |
| Q80643415 | Fast, efficient and reproducible genetic transformation of Phaseolus spp. by Agrobacterium rhizogenes |
| Q39531434 | Faster generation of hiPSCs by coupling high-titer lentivirus and column-based positive selection |
| Q34952899 | Feeder-dependent and feeder-independent iPS cell derivation from human and mouse adipose stem cells |
| Q47363760 | Feeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification |
| Q34748374 | Femtosecond optical transfection of individual mammalian cells. |
| Q96766165 | FiTAc-seq: fixed-tissue ChIP-seq for H3K27ac profiling and super-enhancer analysis of FFPE tissues |
| Q88220506 | Fiber-optic implant for simultaneous fluorescence-based calcium recordings and BOLD fMRI in mice |
| Q28364523 | FibrilTool, an ImageJ plug-in to quantify fibrillar structures in raw microscopy images |
| Q57165488 | Fit-free analysis of fluorescence lifetime imaging data using the phasor approach |
| Q34313305 | Fitness analyses of all possible point mutations for regions of genes in yeast |
| Q84261526 | Flexizymes for genetic code reprogramming |
| Q80092973 | Flow cytometry after bromodeoxyuridine labeling to measure S and G2+M phase durations plus doubling times in vitro and in vivo |
| Q34615436 | Flow cytometry and FISH to measure the average length of telomeres (flow FISH). |
| Q37120375 | Fluid-percussion–induced traumatic brain injury model in rats |
| Q38427746 | Fluorescence and chromogenic in situ hybridization to detect genetic aberrations in formalin-fixed paraffin embedded material, including tissue microarrays |
| Q33298810 | Fluorescence detection of protein clusters in individual cells and tissue sections by using toponome imaging system: sample preparation and measuring procedures |
| Q80643422 | Fluorescence in situ hybridization on vibratome sections of plant tissues |
| Q37131524 | Fluorescence resonance energy transfer microscopy as demonstrated by measuring the activation of the serine/threonine kinase Akt. |
| Q33762078 | Fluorescence-activated cell sorting for aptamer SELEX with cell mixtures |
| Q31142669 | Fluorescent Arabidopsis tetrads: a visual assay for quickly developing large crossover and crossover interference data sets |
| Q39685953 | Fluorescent conjugated polymer-based FRET technique for detection of DNA methylation of cancer cells |
| Q39986166 | Fluorescent high-throughput screening of chemical inducers of neuronal differentiation in skeletal muscle cells. |
| Q30620424 | Fluorescent in situ sequencing (FISSEQ) of RNA for gene expression profiling in intact cells and tissues. |
| Q33848893 | Fluorescent in situ visualization of sterols in Arabidopsis roots. |
| Q44090414 | Fluorescent labeling of nano-sized vesicles released by cells and subsequent quantitative and qualitative analysis by high-resolution flow cytometry |
| Q34913250 | Fluorescent labeling of tetracysteine-tagged proteins in intact cells |
| Q50227859 | Fluorescent mRNA labeling through cytoplasmic FISH. |
| Q51009430 | Fluorescent microsphere technique to measure cerebral blood flow in the rat. |
| Q52926715 | Fluorometric measurement of nitrite/nitrate by 2,3-diaminonaphthalene. |
| Q59723685 | Flux profiling of photosynthetic carbon metabolism in intact plants |
| Q36150661 | Focal embolic cerebral ischemia in the rat. |
| Q81437864 | Footprinting protein-DNA complexes using the hydroxyl radical |
| Q27310375 | Force dependency of biochemical reactions measured by single-molecule force-clamp spectroscopy. |
| Q89637913 | Formaldehyde-assisted isolation of regulatory DNA elements from Arabidopsis leaves |
| Q89687025 | Formation and growth of sub-3-nm aerosol particles in experimental chambers |
| Q45102722 | Formation of microvascular networks in vitro |
| Q91691731 | Fractionation of lignocellulosic biomass to produce uncondensed aldehyde-stabilized lignin |
| Q33280986 | Freeze-fracture electron microscopy |
| Q80805155 | Frontal affinity chromatography-mass spectrometry |
| Q38297217 | Frontal affinity chromatography: sugar-protein interactions |
| Q80078234 | Frozen competent yeast cells that can be transformed with high efficiency using the LiAc/SS carrier DNA/PEG method |
| Q34395363 | Full-length RNA-seq from single cells using Smart-seq2. |
| Q91556285 | Full-length sequencing of circular DNA viruses and extrachromosomal circular DNA using CIDER-Seq |
| Q31172555 | Functional genomics platform for pooled screening and generation of mammalian genetic interaction maps |
| Q44519982 | Functional transient genetic transformation of Arabidopsis leaves by biolistic bombardment. |
| Q51439316 | Functionalization, preparation and use of cell-laden gelatin methacryloyl-based hydrogels as modular tissue culture platforms. |
| Q62658531 | Fusion PCR and gene targeting in Aspergillus nidulans |
| Q41773314 | Fusion of single proteoliposomes with planar, cushioned bilayers in microfluidic flow cells |
| Q37347696 | GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae |
| Q98471385 | GOTI, a method to identify genome-wide off-target effects of genome editing in mouse embryos |
| Q92596498 | GRID-seq for comprehensive analysis of global RNA-chromatin interactions |
| Q56981373 | Gas chromatography mass spectrometry–based metabolite profiling in plants |
| Q37217394 | Gaussia luciferase reporter assay for monitoring biological processes in culture and in vivo |
| Q79763798 | Gel chromatography and analytical ultracentrifugation to determine the extent of detergent binding and aggregation, and Stokes radius of membrane proteins using sarcoplasmic reticulum Ca2+-ATPase as an example |
| Q43286145 | Gel-based mass spectrometric analysis of a strongly hydrophobic GABAA-receptor subunit containing four transmembrane domains |
| Q33600977 | Gene function analysis in complex data sets using ErmineJ. |
| Q40025495 | Gene knockdown by ecdysone-based inducible RNAi in stable mammalian cell lines |
| Q29615338 | Gene splicing and mutagenesis by PCR-driven overlap extension |
| Q37373512 | Generating gene knockout rats by homologous recombination in embryonic stem cells |
| Q46861803 | Generating genetically modified mice using CRISPR/Cas-mediated genome engineering |
| Q33551154 | Generating heparan sulfate saccharide libraries for glycomics applications. |
| Q38728131 | Generating high-purity cardiac and endothelial derivatives from patterned mesoderm using human pluripotent stem cells |
| Q37495400 | Generating human intestinal tissue from pluripotent stem cells in vitro |
| Q39058950 | Generating suspended cell monolayers for mechanobiological studies |
| Q37845594 | Generating whole bacterial genome sequences of low-abundance species from complex samples with IMS-MDA. |
| Q58451614 | Generation and assembly of human brain region-specific three-dimensional cultures. |
| Q101476557 | Generation and biobanking of patient-derived glioblastoma organoids and their application in CAR T cell testing |
| Q113391072 | Generation and characterization of hair-bearing skin organoids from human pluripotent stem cells |
| Q52603774 | Generation and customization of biosynthetic excitable tissues for electrophysiological studies and cell-based therapies. |
| Q82098975 | Generation and differentiation of microtissues from multipotent precursor cells for use in tissue engineering |
| Q48163616 | Generation and isolation of oligodendrocyte progenitor cells from human pluripotent stem cells |
| Q30358248 | Generation and purification of highly specific antibodies for detecting post-translationally modified proteins in vivo. |
| Q46361829 | Generation and use of a humanized bone-marrow-ossicle niche for hematopoietic xenotransplantation into mice |
| Q57981069 | Generation and validation of homozygous fluorescent knock-in cells using CRISPR–Cas9 genome editing |
| Q39099728 | Generation of T cell receptor-retrogenic mice: improved retroviral-mediated stem cell gene transfer |
| Q44690850 | Generation of T-cell receptor retrogenic mice |
| Q58047136 | Generation of a defined and uniform population of CNS progenitors and neurons from mouse embryonic stem cells |
| Q48874047 | Generation of a double-fluorescent double-selectable Cre/loxP indicator vector for monitoring of intracellular recombination events. |
| Q33287827 | Generation of a fluorescently labeled endogenous protein library in living human cells |
| Q37691536 | Generation of a humanized mouse model with both human immune system and liver cells to model hepatitis C virus infection and liver immunopathogenesis. |
| Q34113422 | Generation of a transgenic ORFeome library in Drosophila. |
| Q50693981 | Generation of a vascularized and functional human liver from an iPSC-derived organ bud transplant. |
| Q36957135 | Generation of acetyllysine antibodies and affinity enrichment of acetylated peptides |
| Q40813551 | Generation of adult human induced pluripotent stem cells using nonviral minicircle DNA vectors |
| Q90288972 | Generation of blood vessel organoids from human pluripotent stem cells |
| Q38953390 | Generation of cell-based systems to visualize chromosome damage and translocations in living cells |
| Q51751259 | Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro-formed nuclei for Western blotting and scanning electron microscopy (SEM). |
| Q40150102 | Generation of cells expressing improved doxycycline-regulated reverse transcriptional transactivator rtTA2S-M2. |
| Q28973621 | Generation of cerebral organoids from human pluripotent stem cells |
| Q53251361 | Generation of chemically engineered ribosomes for atomic mutagenesis studies on protein biosynthesis. |
| Q39732178 | Generation of clonal zebrafish lines and transplantable hepatic tumors |
| Q43268255 | Generation of cortical neurons from mouse embryonic stem cells |
| Q48621531 | Generation of eggs from mouse embryonic stem cells and induced pluripotent stem cells. |
| Q110698455 | Generation of heart-forming organoids from human pluripotent stem cells |
| Q47734532 | Generation of high-purity human ventral midbrain dopaminergic progenitors for in vitro maturation and intracerebral transplantation |
| Q93336621 | Generation of human antral and fundic gastric organoids from pluripotent stem cells |
| Q50422331 | Generation of human brain region-specific organoids using a miniaturized spinning bioreactor. |
| Q34310664 | Generation of human induced pluripotent stem cells from urine samples. |
| Q38748704 | Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro |
| Q90776942 | Generation of human oogonia from induced pluripotent stem cells in culture |
| Q34792230 | Generation of human-induced pluripotent stem cells |
| Q39640194 | Generation of induced pluripotent stem cells from a small amount of human peripheral blood using a combination of activated T cells and Sendai virus |
| Q60221837 | Generation of induced pluripotent stem cells from human cord blood cells with only two factors: Oct4 and Sox2 |
| Q48444472 | Generation of induced pluripotent stem cells from neural stem cells. |
| Q37131138 | Generation of integration-free human induced pluripotent stem cells from postnatal blood mononuclear cells by plasmid vector expression |
| Q37420600 | Generation of kidney organoids from human pluripotent stem cells |
| Q86434301 | Generation of living cell arrays for atomic force microscopy studies |
| Q64277444 | Generation of lung organoids from human pluripotent stem cells in vitro |
| Q43419330 | Generation of magnetic nonviral gene transfer agents and magnetofection in vitro |
| Q48548204 | Generation of meiomaps of genome-wide recombination and chromosome segregation in human oocytes. |
| Q53746234 | Generation of microglial cells from mouse embryonic stem cells. |
| Q45013661 | Generation of mouse-induced pluripotent stem cells with plasmid vectors |
| Q43900005 | Generation of multipotent induced cardiac progenitor cells from mouse fibroblasts and potency testing in ex vivo mouse embryos. |
| Q37613263 | Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells |
| Q100958926 | Generation of oligodendrocytes and establishment of an all-human myelinating platform from human pluripotent stem cells |
| Q34372342 | Generation of orthotopic and heterotopic human pancreatic cancer xenografts in immunodeficient mice |
| Q60634999 | Generation of peptide–MHC class I complexes through UV-mediated ligand exchange |
| Q84261535 | Generation of phospholipid vesicle-nanotube networks and transport of molecules therein |
| Q52654029 | Generation of spatial-patterned early-developing cardiac organoids using human pluripotent stem cells. |
| Q83366745 | Generation of subtype-specific neurons from postnatal astroglia of the mouse cerebral cortex |
| Q91740204 | Generation of synthetic nanobodies against delicate proteins |
| Q38311520 | Generation of targeted mouse mutants by embryo microinjection of TALEN mRNA. |
| Q53088533 | Generation of transgene-free human induced pluripotent stem cells with an excisable single polycistronic vector. |
| Q51976447 | Generation of transgenic birds with replication-deficient lentiviruses. |
| Q34836605 | Generation of transgenic mice with megabase-sized human yeast artificial chromosomes by yeast spheroplast-embryonic stem cell fusion |
| Q39184690 | Generation, expansion and functional analysis of endothelial cells and pericytes derived from human pluripotent stem cells |
| Q110597945 | Generation, functional analysis and applications of isogenic three-dimensional self-aggregating cardiac microtissues from human pluripotent stem cells |
| Q111696016 | Generic acquisition protocol for quantitative MRI of the spinal cord |
| Q40704653 | Genetic engineering of untransformable coagulase-negative staphylococcal pathogens |
| Q40050373 | Genetic knockouts and knockins in human somatic cells |
| Q57071383 | Genetic lineage tracing of resident stem cells by DeaLT |
| Q51021823 | Genetic manipulation of Aspergillus nidulans: heterokaryons and diploids for dominance, complementation and haploidization analyses. |
| Q51021829 | Genetic manipulation of Aspergillus nidulans: meiotic progeny for genetic analysis and strain construction. |
| Q57235154 | Genetic transformation of tobacco NT1 cells with Agrobacterium tumefaciens |
| Q47652236 | Genetically encoded releasable photo-cross-linking strategies for studying protein-protein interactions in living cells |
| Q101237628 | Genetically modified mouse models to help fight COVID-19 |
| Q38918318 | Genome editing comes of age. |
| Q50633917 | Genome editing in rice and wheat using the CRISPR/Cas system. |
| Q48125113 | Genome editing of bread wheat using biolistic delivery of CRISPR/Cas9 in vitro transcripts or ribonucleoproteins. |
| Q38716194 | Genome editing using FACS enrichment of nuclease-expressing cells and indel detection by amplicon analysis |
| Q22122027 | Genome engineering using the CRISPR-Cas9 system |
| Q34553929 | Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening |
| Q35516152 | Genome-scale RNAi screens for high-throughput phenotyping in bloodstream-form African trypanosomes |
| Q35037090 | Genome-scale analysis of replication timing: from bench to bioinformatics. |
| Q45981330 | Genome-wide analysis of barcoded Saccharomyces cerevisiae gene-deletion mutants in pooled cultures. |
| Q51415384 | Genome-wide analysis of replication timing by next-generation sequencing with E/L Repli-seq. |
| Q36275051 | Genome-wide base-resolution mapping of DNA methylation in single cells using single-cell bisulfite sequencing (scBS-seq). |
| Q54598378 | Genome-wide chromatin analysis in mature mouse and human spermatozoa. |
| Q30372139 | Genome-wide copy number analysis of single cells. |
| Q101166878 | Genome-wide detection of DNA double-strand breaks by in-suspension BLISS |
| Q45862487 | Genome-wide high-throughput integrome analyses by nrLAM-PCR and next-generation sequencing |
| Q87411621 | Genome-wide identification and quantification of protein synthesis in cultured cells and whole tissues by puromycin-associated nascent chain proteomics (PUNCH-P) |
| Q33950714 | Genome-wide identification of transcript start and end sites by transcript isoform sequencing |
| Q42796095 | Genome-wide mapping of DNase I hypersensitive sites in rare cell populations using single-cell DNase sequencing |
| Q34655867 | Genome-wide mapping of RNA structure using nuclease digestion and high-throughput sequencing |
| Q41819956 | Genome-wide mapping of embedded ribonucleotides and other noncanonical nucleotides using emRiboSeq and EndoSeq. |
| Q51419224 | Genome-wide mapping of endogenous G-quadruplex DNA structures by chromatin immunoprecipitation and high-throughput sequencing. |
| Q90526979 | Genome-wide mapping of nucleotide excision repair with XR-seq |
| Q44006423 | Genome-wide measurement of protein-DNA binding dynamics using competition ChIP. |
| Q97587581 | Genome-wide piggyBac transposon-based mutagenesis and quantitative insertion-site analysis in haploid Candida species |
| Q35969015 | Genome-wide profiling of RNA polymerase transcription at nucleotide resolution in human cells with native elongating transcript sequencing. |
| Q35667041 | Genome-wide profiling of in vivo RNA structure at single-nucleotide resolution using structure-seq. |
| Q91974077 | Genome-wide profiling of nucleosome position and chromatin accessibility in single cells using scMNase-seq |
| Q35907181 | Genome-wide quantification of 5'-phosphorylated mRNA degradation intermediates for analysis of ribosome dynamics |
| Q36247197 | Genome-wide transposon screening and quantitative insertion site sequencing for cancer gene discovery in mice. |
| Q33280964 | Genomic systematic evolution of ligands by exponential enrichment (Genomic SELEX) for the identification of protein-binding RNAs independent of their expression levels |
| Q36479375 | Genomic variant annotation and prioritization with ANNOVAR and wANNOVAR. |
| Q97424693 | Geometric morphometrics of microscopic animals as exemplified by model nematodes |
| Q42231746 | Germline transgenesis in pigs by cytoplasmic microinjection of Sleeping Beauty transposons. |
| Q42231749 | Germline transgenesis in rabbits by pronuclear microinjection of Sleeping Beauty transposons. |
| Q42231754 | Germline transgenesis in rodents by pronuclear microinjection of Sleeping Beauty transposons. |
| Q102152485 | Global analysis of RNA-binding protein dynamics by comparative and enhanced RNA interactome capture |
| Q100958929 | Global analysis of repetitive DNA from unassembled sequence reads using RepeatExplorer2 |
| Q42212790 | Global identification of protein kinase substrates by protein microarray analysis |
| Q46762192 | Global metabolic profiling of animal and human tissues via UPLC-MS. |
| Q58045640 | Global metabolic profiling procedures for urine using UPLC–MS |
| Q34615456 | Global single-cell cDNA amplification to provide a template for representative high-density oligonucleotide microarray analysis |
| Q53068939 | Global site-specific analysis of glycoprotein N-glycan processing. |
| Q58045595 | Global urinary metabolic profiling procedures using gas chromatography–mass spectrometry |
| Q36169705 | Global, in situ, site-specific analysis of protein S-sulfenylation |
| Q49057061 | Glycome mapping on DNA sequencing equipment. |
| Q90814940 | Guidance for quantitative confocal microscopy |
| Q54508514 | Guide to video recording of structure dynamics and dynamic processes of proteins by high-speed atomic force microscopy. |
| Q49020806 | Guidelines for the automated evaluation of Elispot assays. |
| Q93012478 | Guidelines for using sigQC for systematic evaluation of gene signatures |
| Q62779852 | Guiding biomedical clustering with ClustEval |
| Q48722962 | Head-anchored whole-cell recordings in freely moving rats. |
| Q48166193 | Heat-induced radiolabeling and fluorescence labeling of Feraheme nanoparticles for PET/SPECT imaging and flow cytometry |
| Q34732161 | Hematopoietic differentiation and production of mature myeloid cells from human pluripotent stem cells |
| Q38303176 | Heteroduplex analysis by capillary array electrophoresis for rapid mutation detection in large multiexon genes. |
| Q80093481 | Heterotopic vascularized murine cardiac transplantation to study graft arteriopathy |
| Q109931231 | Hierarchical imaging and computational analysis of three-dimensional vascular network architecture in the entire postnatal and adult mouse brain |
| Q48218879 | High Ca2+-phosphate transfection efficiency in low-density neuronal cultures |
| Q44172021 | High efficiency germ-line transformation of mosquitoes |
| Q80093384 | High resolution colonoscopy in live mice |
| Q91272687 | High spatial resolution imaging of biological tissues using nanospray desorption electrospray ionization mass spectrometry |
| Q36981939 | High-contrast en bloc staining of neuronal tissue for field emission scanning electron microscopy |
| Q91557881 | High-dimensional cell-level analysis of tissues with Ce3D multiplex volume imaging |
| Q28296322 | High-efficiency transfection and drug selection of genetically transformed blood stages of the rodent malaria parasite Plasmodium berghei |
| Q48099994 | High-efficiency transfection of mammalian neurons via nucleofection |
| Q46715832 | High-efficiency transformation by biolistics of soybean, common bean and cotton transgenic plants |
| Q29615751 | High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method |
| Q91081335 | High-fidelity probing of the structure and heterogeneity of extracellular vesicles by resonance-enhanced atomic force microscopy infrared spectroscopy |
| Q36077253 | High-mass-resolution MALDI mass spectrometry imaging of metabolites from formalin-fixed paraffin-embedded tissue |
| Q35984139 | High-performance liquid chromatography-mass spectrometry for mapping and sequencing glycosaminoglycan-derived oligosaccharides |
| Q36094995 | High-quality full-length immunoglobulin profiling with unique molecular barcoding. |
| Q93023775 | High-resolution 13C metabolic flux analysis |
| Q40563361 | High-resolution 3D analysis of mouse small-intestinal stroma |
| Q92565031 | High-resolution 3D imaging and analysis of axon regeneration in unsectioned spinal cord with or without tissue clearing |
| Q91752377 | High-resolution 3D imaging of fixed and cleared organoids |
| Q51575247 | High-resolution circular chromosome conformation capture assay. |
| Q33366978 | High-resolution human papillomavirus genotyping by MALDI-TOF mass spectrometry |
| Q119529106 | High-resolution imaging of bacterial spatial organization with vertical cell imaging by nanostructured immobilization (VerCINI) |
| Q29617529 | High-resolution in situ hybridization to whole-mount zebrafish embryos |
| Q44330989 | High-resolution magic-angle-spinning NMR spectroscopy for metabolic profiling of intact tissues |
| Q43972485 | High-resolution membrane capacitance measurements for the study of exocytosis and endocytosis. |
| Q34320668 | High-resolution optical control of spatiotemporal neuronal activity patterns in zebrafish using a digital micromirror device. |
| Q91752343 | High-resolution, strand-specific R-loop mapping via S9.6-based DNA-RNA immunoprecipitation and high-throughput sequencing |
| Q40348241 | High-sensitivity infrared attenuated total reflectance sensors for in situ multicomponent detection of volatile organic compounds in water |
| Q93112689 | High-speed imaging of glutamate release with genetically encoded sensors |
| Q104472045 | High-speed super-resolution imaging of rotationally symmetric structures using SPEED microscopy and 2D-to-3D transformation |
| Q39709060 | High-stringency tandem affinity purification of proteins conjugated to ubiquitin-like moieties |
| Q53087211 | High-throughput analysis of meiotic crossover frequency and interference via flow cytometry of fluorescent pollen in Arabidopsis thaliana. |
| Q91322455 | High-throughput and high-sensitivity phosphoproteomics with the EasyPhos platform |
| Q42619744 | High-throughput and sensitive assay to measure yeast cell growth: a bench protocol for testing genotoxic agents |
| Q96431808 | High-throughput assay for determining enantiomeric excess of chiral diols, amino alcohols, and amines and for direct asymmetric reaction screening |
| Q38303102 | High-throughput assay for determining specificity and affinity of protein-DNA binding interactions |
| Q35096003 | High-throughput ballistic injection nanorheology to measure cell mechanics |
| Q34594554 | High-throughput chromatin immunoprecipitation for genome-wide mapping of in vivo protein-DNA interactions and epigenomic states |
| Q90288984 | High-throughput detection and tracking of cells and intracellular spots in mother machine experiments |
| Q42082468 | High-throughput fluorescence assay of cytochrome P450 3A4. |
| Q28817960 | High-throughput fluorescence-based isolation of live C. elegans larvae |
| Q40597822 | High-throughput haplotype determination over long distances by haplotype fusion PCR and ligation haplotyping |
| Q35731284 | High-throughput identification of proteins with AMPylation using self-assembled human protein (NAPPA) microarrays |
| Q89435099 | High-throughput imaging flow cytometry by optofluidic time-stretch microscopy |
| Q35994611 | High-throughput imaging of adult fluorescent zebrafish with an LED fluorescence macroscope |
| Q47210490 | High-throughput in situ X-ray screening of and data collection from protein crystals at room temperature and under cryogenic conditions. |
| Q42408296 | High-throughput knockout screen in fission yeast |
| Q101166887 | High-throughput matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry-based deubiquitylating enzyme assay for drug discovery |
| Q38981063 | High-throughput microfluidic single-cell analysis pipeline for studies of signaling dynamics |
| Q27310343 | High-throughput microfluidics to control and measure signaling dynamics in single yeast cells |
| Q42844147 | High-throughput microplate assay for the determination of drug partition coefficients |
| Q56379600 | High-throughput microsphiltration to assess red blood cell deformability and screen for malaria transmission-blocking drugs |
| Q93001365 | High-throughput multicolor optogenetics in microwell plates |
| Q91362876 | High-throughput phenotyping with electronic medical record data using a common semi-supervised approach (PheCAP) |
| Q33280945 | High-throughput plasmid cDNA library screening |
| Q54443844 | High-throughput screening of activity and enantioselectivity of esterases. |
| Q33283982 | High-throughput screening of small molecules for bioactivity and target identification in Caenorhabditis elegans |
| Q33645375 | High-throughput screening of tissue-nonspecific alkaline phosphatase for identification of effectors with diverse modes of action |
| Q30443589 | High-throughput transgenesis in Xenopus using I-SceI meganuclease |
| Q36593944 | High-throughput, detailed, cell-specific neuroanatomy of dendritic spines using microinjection and confocal microscopy |
| Q80829957 | High-throughput, nontargeted metabolite fingerprinting using nominal mass flow injection electrospray mass spectrometry |
| Q27310369 | High-yield and high-purity isolation of hepatic stellate cells from normal and fibrotic mouse livers |
| Q53486783 | High-yield cell-free protein production from P-gel. |
| Q57910561 | High-yield in vitro recordings from neurons functionally characterized in vivo |
| Q122150020 | High-yield, green and scalable methods for producing MOF-303 for water harvesting from desert air |
| Q39486563 | Highly efficient subcloning of rodent malaria parasites by injection of single merosomes or detached cells |
| Q90197793 | Highly enantioselective rhodium-catalyzed cross-coupling of boronic acids and racemic allyl halides |
| Q47936785 | Highly multiplexed and strand-specific single-cell RNA 5' end sequencing |
| Q30364660 | Highly multiplexed targeted DNA sequencing from single nuclei |
| Q80093329 | Highly purified selective isolation of eosinophils from human peripheral blood by negative immunomagnetic selection |
| Q57279159 | Highly selective enrichment of phosphorylated peptides using titanium dioxide |
| Q91908228 | Holographic two-photon activation for synthetic optogenetics |
| Q46445881 | Homogenization of sample absorption for the imaging of large and dense fossils with synchrotron microtomography. |
| Q30497861 | Housing conditions and stimulus females: a robust social discrimination task for studying male rodent social recognition |
| Q50276957 | Housing, husbandry and handling of rodents for behavioral experiments. |
| Q44076546 | Human and rat adult Schwann cell cultures: fast and efficient enrichment and highly effective non-viral transfection protocol |
| Q34938752 | Human cardiomyocyte progenitor cells differentiate into functional mature cardiomyocytes: an in vitro model for studying human cardiac physiology and pathophysiology. |
| Q36399672 | Human norovirus culture in B cells |
| Q46139897 | Hybrid recreation by reverse breeding in Arabidopsis thaliana |
| Q30478597 | Hybrid selection of discrete genomic intervals on custom-designed microarrays for massively parallel sequencing |
| Q36631756 | Hydrodynamic size-based separation and characterization of protein aggregates from total cell lysates |
| Q86872292 | Hydrogenation using iron oxide-based nanocatalysts for the synthesis of amines |
| Q39624502 | Hypoxia-induced metastasis model in embryonic zebrafish |
| Q50532574 | Hypoxia-induced retinopathy model in adult zebrafish. |
| Q24605680 | I-TASSER: a unified platform for automated protein structure and function prediction |
| Q92461268 | INFOGEST static in vitro simulation of gastrointestinal food digestion |
| Q80093298 | IRAP and REMAP for retrotransposon-based genotyping and fingerprinting |
| Q41619122 | ISH-IEM: a sensitive method to detect endogenous mRNAs at the ultrastructural level |
| Q87483507 | Identification and analysis of endogenous SUMO1 and SUMO2/3 targets in mammalian cells and tissues using monoclonal antibodies |
| Q61513255 | Identification and analysis of essential Aspergillus nidulans genes using the heterokaryon rescue technique |
| Q88488766 | Identification and characterization of nucleobase-modified aptamers by click-SELEX |
| Q33280947 | Identification and detection of RNA-RNA interactions using the yeast RNA hybrid system |
| Q80422745 | Identification and insertion of 3-carbon bridges in protein disulfide bonds: a computational approach |
| Q50483075 | Identification and propagation of haploid human pluripotent stem cells. |
| Q44973552 | Identification and quantification of aminophospholipid molecular species on the surface of apoptotic and activated cells |
| Q41831149 | Identification of N-terminally arginylated proteins and peptides by mass spectrometry |
| Q47449956 | Identification of RNA-binding domains of RNA-binding proteins in cultured cells on a system-wide scale with RBDmap. |
| Q94229433 | Identification of RNA-binding protein targets with HyperTRIBE |
| Q110698454 | Identification of cancer-related mutations in human pluripotent stem cells using RNA-seq analysis |
| Q47630337 | Identification of cross talk between SUMOylation and ubiquitylation using a sequential peptide immunopurification approach |
| Q33303175 | Identification of enzymes and activity from two-dimensional gel electrophoresis |
| Q33280971 | Identification of eukaryotic secreted and cell surface proteins using the yeast secretion trap screen |
| Q50699994 | Identification of fungal cell wall mutants using susceptibility assays based on Calcofluor white and Congo red. |
| Q50072446 | Identification of host-induced pathogen genes by differential fluorescence induction reporter systems. |
| Q80093361 | Identification of membrane proteins from mammalian cell/tissue using methanol-facilitated solubilization and tryptic digestion coupled with 2D-LC-MS/MS |
| Q38299143 | Identification of target mRNAs of regulatory RNA-binding proteins using mRNP immunopurification and microarrays |
| Q42283389 | Identification, isolation and in vitro expansion of human and nonhuman primate T stem cell memory cells. |
| Q89867107 | Identification, quantification and bioinformatic analysis of RNA-dependent proteins by RNase treatment and density gradient ultracentrifugation using R-DeeP |
| Q42665464 | Identifying ChIP-seq enrichment using MACS. |
| Q33280931 | Identifying DNA sequences recognized by a transcription factor using a bacterial one-hybrid system |
| Q34220314 | Identifying and quantifying proteolytic events and the natural N terminome by terminal amine isotopic labeling of substrates |
| Q52560090 | Identifying key membrane protein lipid interactions using mass spectrometry. |
| Q30511949 | Identifying microbial fitness determinants by insertion sequencing using genome-wide transposon mutant libraries |
| Q33286527 | Identifying specificity profiles for peptide recognition modules from phage-displayed peptide libraries |
| Q92933006 | Identifying statistically significant chromatin contacts from Hi-C data with FitHiC2 |
| Q97587578 | Identifying unknown metabolites using NMR-based metabolic profiling techniques |
| Q35636148 | Illumina human exome genotyping array clustering and quality control |
| Q33341061 | Image processing for electron microscopy single-particle analysis using XMIPP. |
| Q46850593 | Imaging activation of adult-generated granule cells in spatial memory |
| Q33408754 | Imaging biological structures with fluorescence photoactivation localization microscopy |
| Q98513739 | Imaging endocytic vesicle formation at high spatial and temporal resolutions with the pulsed-pH protocol |
| Q35833685 | Imaging fluorescence (cross-) correlation spectroscopy in live cells and organisms. |
| Q35539946 | Imaging liver biology in vivo using conventional confocal microscopy. |
| Q33291319 | Imaging malaria sporozoites in the dermis of the mammalian host |
| Q33286534 | Imaging mass spectrometry at cellular length scales |
| Q91303043 | Imaging neuromodulators with high spatiotemporal resolution using genetically encoded indicators |
| Q50490679 | Imaging of neutral lipids by oil red O for analyzing the metabolic status in health and disease. |
| Q47228001 | Imaging of plant cell walls by confocal Raman microscopy |
| Q34066570 | Imaging of rat optic nerve axons in vivo |
| Q87113988 | Imaging pericytes and capillary diameter in brain slices and isolated retinae |
| Q51504985 | Imaging protein activity in live embryos using fluorescence resonance energy transfer biosensors. |
| Q37164001 | Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin |
| Q33280978 | Imaging synaptic vesicle exocytosis and endocytosis with FM dyes |
| Q30843992 | Imaging the beating heart in the mouse using intravital microscopy techniques. |
| Q33280974 | Imaging the lateral diffusion of membrane molecules with quantum dots |
| Q33920734 | Imaging three-dimensional tissue architectures by focused ion beam scanning electron microscopy. |
| Q50989029 | Immobilization of enzymes on heterofunctional epoxy supports. |
| Q84387294 | Immobilization of growth factors on solid supports for the modulation of stem cell fate |
| Q40399099 | Immuno-engineered organoids for regulating the kinetics of B-cell development and antibody production. |
| Q37264945 | Immuno-spin trapping analyses of DNA radicals |
| Q80092785 | Immunocytochemical technique for protein localization in sections of plant tissues |
| Q80092782 | Immunocytochemical techniques for whole-mount in situ protein localization in plants |
| Q46891143 | Immunocytochemistry and quantification of protein colocalization in cultured neurons |
| Q37368111 | Immunodesign of experimental sepsis by cecal ligation and puncture |
| Q33283980 | Immunofluorescence in brain sections: simultaneous detection of presynaptic and postsynaptic proteins in identified neurons. |
| Q48913311 | Immunofluorescence microscopy and immunogold EM for investigating fungal infection of plants. |
| Q39139897 | Immunofluorescent antibody staining of intact Drosophila larvae |
| Q52678555 | Immunofluorescent staining and imaging of the pupal and adult Drosophila visual system. |
| Q46809743 | Immunogold quantification of amino acids and proteins in complex subcellular compartments |
| Q33280976 | Immunohistochemical staining of post-mortem adult human brain sections. |
| Q48218889 | Immunoprecipitation of mRNA-protein complexes |
| Q111442045 | Implementation of a 4Pi-SMS super-resolution microscope |
| Q48678364 | Improved biocytin labeling and neuronal 3D reconstruction. |
| Q104073805 | Improved high-molecular-weight DNA extraction, nanopore sequencing and metagenomic assembly from the human gut microbiome |
| Q46553997 | Improved northern blot method for enhanced detection of small RNA. |
| Q38804973 | Improving your four-dimensional image: traveling through a decade of light-sheet-based fluorescence microscopy research |
| Q46812961 | In situ and real-time monitoring of mechanochemical milling reactions using synchrotron X-ray diffraction |
| Q51417500 | In situ cultivation of previously uncultivable microorganisms using the ichip. |
| Q54454420 | In situ detection of individual mRNA molecules and protein complexes or post-translational modifications using padlock probes combined with the in situ proximity ligation assay. |
| Q60023998 | In situ hybridization for mRNA detection in Arabidopsis tissue sections |
| Q80285948 | In situ hybridization technique for mRNA detection in whole mount Arabidopsis samples |
| Q83506128 | In situ localization of small RNAs in plants by using LNA probes |
| Q91666620 | In situ observation of conformational dynamics and protein ligand-substrate interactions in outer-membrane proteins with DEER/PELDOR spectroscopy |
| Q53492928 | In situ simultaneous monitoring of ATP and GTP using a graphene oxide nanosheet-based sensing platform in living cells. |
| Q33283979 | In vitro 'sexual' evolution through the PCR-based staggered extension process (StEP). |
| Q28264802 | In vitro and in vivo generation and characterization of Pseudomonas aeruginosa biofilm-dispersed cells via c-di-GMP manipulation |
| Q83181157 | In vitro angiogenesis: endothelial cell tube formation on gelled basement membrane extract |
| Q33280982 | In vitro assay for the quantitation of apoptosis-induced alterations of nuclear envelope permeability |
| Q53195425 | In vitro assay to determine SUMOylation sites on protein substrates. |
| Q36901305 | In vitro bioengineered model of cortical brain tissue |
| Q93081321 | In vitro conversion of adult murine endothelial cells to hematopoietic stem cells |
| Q84618855 | In vitro culture and expansion of human limbal epithelial cells |
| Q30532002 | In vitro culture of epicardial cells from adult zebrafish heart on a fibrin matrix |
| Q67596543 | In vitro culture of mouse blastocysts beyond the implantation stages |
| Q90929589 | In vitro establishment of expanded-potential stem cells from mouse pre-implantation embryos or embryonic stem cells |
| Q37672580 | In vitro expansion and genetic modification of gastrointestinal stem cells in spheroid culture. |
| Q36790489 | In vitro functional imaging in brain slices using fast voltage-sensitive dye imaging combined with whole-cell patch recording. |
| Q80643410 | In vitro generation of CD4+ CD25+ regulatory cells from murine naive T cells |
| Q80285996 | In vitro generation of germ cells from murine embryonic stem cells |
| Q89486205 | In vitro generation of mouse polarized embryo-like structures from embryonic and trophoblast stem cells |
| Q33404034 | In vitro growth and analysis of Candida biofilms |
| Q43689879 | In vitro imaging of primary neural cell culture from Drosophila. |
| Q27310402 | In vitro imaging using laser photostimulation with flavoprotein autofluorescence. |
| Q33280937 | In vitro measurement of cell death with the annexin A5 affinity assay |
| Q80422726 | In vitro methods to prepare astrocyte and motoneuron cultures for the investigation of potential in vivo interactions |
| Q38805907 | In vitro reconstitution of B cell receptor-antigen interactions to evaluate potential vaccine candidates. |
| Q29617927 | In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro |
| Q36176672 | In vitro screening for molecules that affect virus capsid assembly (and other protein association reactions) |
| Q82793585 | In vitro screens for quorum sensing inhibitors and in vivo confirmation of their effect |
| Q39088044 | In vitro sperm production from mouse spermatogonial stem cell lines using an organ culture method |
| Q85845947 | In vivo MRI virtual colonography in a mouse model of colon cancer |
| Q42212040 | In vivo antigen-driven plasmablast enrichment in combination with antigen-specific cell sorting to facilitate the isolation of rare monoclonal antibodies from human B cells |
| Q97587577 | In vivo compression and imaging in mouse brain to measure the effects of solid stress |
| Q43268253 | In vivo dendritic calcium imaging with a fiberoptic periscope system |
| Q48218778 | In vivo electroporation in the embryonic mouse central nervous system |
| Q46069567 | In vivo evaluation of human hematopoiesis through xenotransplantation of purified hematopoietic stem cells from umbilical cord blood |
| Q59784798 | In vivo imaging and analysis of cerebrovascular hemodynamic responses and tissue oxygenation in the mouse brain |
| Q33291322 | In vivo imaging of malaria parasites in the murine liver |
| Q50690780 | In vivo imaging of single axons in the mouse spinal cord. |
| Q51021833 | In vivo imaging of subcutaneous structures using functional photoacoustic microscopy. |
| Q42119768 | In vivo imaging of transplanted hematopoietic stem and progenitor cells in mouse calvarium bone marrow |
| Q48909607 | In vivo imaging of vesicle motion and release at the Drosophila neuromuscular junction. |
| Q42222478 | In vivo introduction of transgenes into mouse sciatic nerve cells in situ using viral vectors |
| Q46951405 | In vivo models of proliferative vitreoretinopathy |
| Q38760712 | In vivo monitoring of cellular energy metabolism using SoNar, a highly responsive sensor for NAD(+)/NADH redox state |
| Q60192352 | In vivo multimodal imaging of transplanted pancreatic islets |
| Q30423888 | In vivo optical molecular imaging and analysis in mice using dorsal window chamber models applied to hypoxia, vasculature and fluorescent reporters. |
| Q57912098 | In vivo protein biotinylation and sample preparation for the proteomic identification of organ- and disease-specific antigens accessible from the vasculature |
| Q91785772 | In vivo pulse labeling of isochronic cohorts of cells in the central nervous system using FlashTag |
| Q41618743 | In vivo rapid gene delivery into postmitotic neocortical neurons using iontoporation |
| Q35439315 | In vivo shRNA screens in solid tumors. |
| Q48218808 | In vivo single-cell electroporation for transfer of DNA and macromolecules. |
| Q48243193 | In vivo single-cell excitability probing of neuronal ensembles in the intact and awake developing Xenopus brain |
| Q84261586 | In vivo solid-phase microextraction for monitoring intravenous concentrations of drugs and metabolites |
| Q50529146 | In vivo two-photon imaging of sensory-evoked dendritic calcium signals in cortical neurons. |
| Q54443874 | In-cell NMR for protein-protein interactions (STINT-NMR). |
| Q88562686 | In-cell RNA structure probing with SHAPE-MaP |
| Q48285708 | In-cell ¹³C NMR spectroscopy for the study of intrinsically disordered proteins. |
| Q29547583 | In-gel digestion for mass spectrometric characterization of proteins and proteomes |
| Q57176233 | In-gel stable isotope labeling for relative quantification using mass spectrometry |
| Q42787771 | Incorporation of a viral DNA-packaging motor channel in lipid bilayers for real-time, single-molecule sensing of chemicals and double-stranded DNA. |
| Q88346536 | Incorporation of isotopic, fluorescent, and heavy-atom-modified nucleotides into RNAs by position-selective labeling of RNA |
| Q39346450 | Increased initiation and growth of tumor cell lines, cancer stem cells and biopsy material in mice using basement membrane matrix protein (Cultrex or Matrigel) co-injection. |
| Q31142768 | Indel variant analysis of short-read sequencing data with Scalpel. |
| Q42931691 | Inducible gene targeting in the neonatal vasculature and analysis of retinal angiogenesis in mice |
| Q104073812 | Inducing different severities of traumatic brain injury in Drosophila using a piezoelectric actuator |
| Q41871966 | Inducing nonsense suppression by targeted pseudouridylation |
| Q39781744 | Induction of asymmetrical cell division to analyze spindle-dependent organelle partitioning using correlative microscopy techniques |
| Q92126199 | Induction of neoantigen-reactive T cells from healthy donors |
| Q48770547 | Induction of pluripotent stem cells from fibroblast cultures. |
| Q51039837 | Inert coupling of IRDye800CW and zirconium-89 to monoclonal antibodies for single- or dual-mode fluorescence and PET imaging. |
| Q51009444 | Inexpensive low-oxygen incubators. |
| Q30299952 | Inexpensive, rapid prototyping of microfluidic devices using overhead transparencies and a laser print, cut and laminate fabrication method |
| Q30367880 | Influenza A virus isolation, culture and identification |
| Q33298808 | Inhibitor fingerprinting of metalloproteases using microplate and microarray platforms: an enabling technology in Catalomics |
| Q57057923 | Initiation, growth and cryopreservation of plant cell suspension cultures |
| Q50861891 | Injection-mediated transposon transgenesis in Xenopus tropicalis and the identification of integration sites by modified extension primer tag selection (EPTS) linker-mediated PCR. |
| Q50191558 | Integrated analysis and reconstruction of microbial transcriptional gene regulatory networks using CoryneRegNet. |
| Q57520343 | Integrated analysis of anatomical and electrophysiological human intracranial data |
| Q31031694 | Integrated analysis of shotgun proteomic data with PatternLab for proteomics 4.0. |
| Q87434200 | Integrated culture and purification of rat Schwann cells from freshly isolated adult tissue |
| Q52431517 | Integrated design, execution, and analysis of arrayed and pooled CRISPR genome-editing experiments. |
| Q36368396 | Integrated mass spectrometry-based analysis of plasma glycoproteins and their glycan modifications |
| Q90663965 | Integrating hydrogen-deuterium exchange mass spectrometry with molecular dynamics simulations to probe lipid-modulated conformational changes in membrane proteins |
| Q36388988 | Integrating macromolecular X-ray diffraction data with the graphical user interface iMosflm. |
| Q31071302 | Integration and global analysis of isothermal titration calorimetry data for studying macromolecular interactions |
| Q28681323 | Integration of biological networks and gene expression data using Cytoscape |
| Q64117207 | Integrative analysis of pooled CRISPR genetic screens using MAGeCKFlute |
| Q47138029 | Interfacing 3D magnetic twisting cytometry with confocal fluorescence microscopy to image force responses in living cells |
| Q35944296 | Interferometric scattering microscopy and its combination with single-molecule fluorescence imaging |
| Q80093212 | Intracellular cytokine optimization and standard operating procedure |
| Q40087876 | Intracellular directed evolution of proteins from combinatorial libraries based on conditional phage replication |
| Q100526352 | Intracellular neuronal recording in awake nonhuman primates |
| Q28257589 | Intracranial self-stimulation (ICSS) in rodents to study the neurobiology of motivation |
| Q35596407 | Intravascular staining for discrimination of vascular and tissue leukocytes. |
| Q30503845 | Intravital imaging of embryonic and tumor neovasculature using viral nanoparticles |
| Q27310352 | Intravital imaging of hair follicle regeneration in the mouse |
| Q58225665 | Intravital multiphoton imaging of immune responses in the mouse ear skin |
| Q93184613 | Introducing our Tutorials |
| Q92886064 | Introducing the new Protocol Exchange site |
| Q47625436 | Intubation-based anesthesia for long-term time-lapse imaging of adult zebrafish |
| Q114554461 | Inverse electron demand Diels–Alder click chemistry for pretargeted PET imaging and radioimmunotherapy |
| Q50280462 | Investigating ADAMTS-mediated aggrecanolysis in mouse cartilage. |
| Q93040388 | Investigating RNA editing in deep transcriptome datasets with REDItools and REDIportal |
| Q47699802 | Investigating conversion of mechanical force into biochemical signaling in three-dimensional chondrocyte cultures |
| Q57126036 | Investigating electron-transfer processes using a biomimetic hybrid bilayer membrane system |
| Q50574419 | Investigating endothelial invasion and sprouting behavior in three-dimensional collagen matrices. |
| Q48796099 | Investigating macromolecules inside cultured and injected cells by in-cell NMR spectroscopy. |
| Q46397170 | Investigating mitotic spindle assembly and function in vitro using Xenopus laevis egg extracts |
| Q30428161 | Investigating protein-protein interactions in living cells using fluorescence lifetime imaging microscopy |
| Q84135021 | Investigation of bacterial chemotaxis in flow-based microfluidic devices |
| Q52666972 | Investigation of higher-order RNA G-quadruplex structures in vitro and in living cells by 19F NMR spectroscopy. |
| Q50579137 | Investigation of mRNA quadruplex formation in Escherichia coli. |
| Q50117371 | Investigation of the spatial structure and interactions of the genome at sub-kilobase-pair resolution using T2C. |
| Q61996163 | Ion mobility–mass spectrometry analysis of large protein complexes |
| Q33280948 | Isolating and engineering human antibodies using yeast surface display |
| Q44260625 | Isolating lipid droplets from multiple species. |
| Q92179639 | Isolating the human cochlea to generate bone powder for ancient DNA analysis |
| Q52947072 | Isolation and analysis of group 2 innate lymphoid cells in mice. |
| Q34125957 | Isolation and characterization of mouse and human esophageal epithelial cells in 3D organotypic culture |
| Q54343615 | Isolation and characterization of resident endogenous c-Kit+ cardiac stem cells from the adult mouse and rat heart. |
| Q57962393 | Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells |
| Q40958518 | Isolation and cultivation of naive-like human pluripotent stem cells based on HERVH expression |
| Q50604691 | Isolation and cultivation of stem cells from adult mouse testes. |
| Q48147846 | Isolation and culture of adult neurons and neurospheres |
| Q37088047 | Isolation and culture of endothelial cells, pericytes and perivascular resident macrophage-like melanocytes from the young mouse ear. |
| Q51984231 | Isolation and culture of rat and mouse oligodendrocyte precursor cells. |
| Q60215185 | Isolation and enrichment of embryonic mouse motoneurons from the lumbar spinal cord of individual mouse embryos |
| Q46777341 | Isolation and expansion of human and mouse brain microvascular endothelial cells |
| Q59754895 | Isolation and functional assessment of mouse skeletal stem cell lineage |
| Q43432170 | Isolation and in vitro culture of primary cardiomyocytes from adult zebrafish hearts |
| Q80829934 | Isolation and propagation of HIV-1 on peripheral blood mononuclear cells |
| Q92173300 | Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue |
| Q44365690 | Isolation and purification of afferent lymph dendritic cells that drain the skin of cattle |
| Q81201348 | Isolation and short-term culture of primary keratinocytes, hair follicle populations and dermal cells from newborn mice and keratinocytes from adult mice for in vitro analysis and for grafting to immunodeficient mice |
| Q50463855 | Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue. |
| Q80093312 | Isolation of Arabidopsis nuclei and measurement of gene transcription rates using nuclear run-on assays |
| Q80093379 | Isolation of CD4+ T cells from murine lungs: a method to analyze ongoing immune responses in the lung |
| Q33280965 | Isolation of Caenorhabditis elegans gene knockouts by PCR screening of chemically mutagenized libraries |
| Q52578162 | Isolation of DNA from bacterial samples of the human gastrointestinal tract. |
| Q38700461 | Isolation of DNA from small amounts of elephant ivory |
| Q44296880 | Isolation of DNA, RNA and protein from the starlet sea anemone Nematostella vectensis |
| Q52578164 | Isolation of RNA from bacterial samples of the human gastrointestinal tract. |
| Q81932797 | Isolation of a slowly adhering cell fraction containing stem cells from murine skeletal muscle by the preplate technique |
| Q39205866 | Isolation of adipose-derived stem cells and their induction to a chondrogenic phenotype. |
| Q34049012 | Isolation of carbohydrate-specific CD4(+) T cell clones from mice after stimulation by two model glycoconjugate vaccines |
| Q83146439 | Isolation of circular yeast artificial chromosomes for synthetic biology and functional genomics studies |
| Q51954306 | Isolation of endothelial cells from fresh tissues. |
| Q36833296 | Isolation of human monoclonal antibodies from peripheral blood B cells |
| Q81246997 | Isolation of intact RNA from cytometrically sorted Saccharomyces cerevisiae for the analysis of intrapopulation diversity of gene expression |
| Q46229127 | Isolation of intact vacuoles from Arabidopsis rosette leaf-derived protoplasts |
| Q49122646 | Isolation of mitochondria from rat brain using Percoll density gradient centrifugation. |
| Q60543070 | Isolation of mitochondria-associated membranes and mitochondria from animal tissues and cells |
| Q50500441 | Isolation of mouse mesenchymal stem cells on the basis of expression of Sca-1 and PDGFR-α. |
| Q30539757 | Isolation of multipotent neural stem or progenitor cells from both the dentate gyrus and subventricular zone of a single adult mouse. |
| Q33283985 | Isolation of murine microglial cells for RNA analysis or flow cytometry |
| Q60542901 | Isolation of plasma membrane–associated membranes from rat liver |
| Q80422705 | Isolation of renal proximal tubular brush-border membranes |
| Q92825814 | Isolation of satellite cells and transplantation into mice for lineage tracing in muscle |
| Q27310327 | Isolation of skeletal muscle stem cells by fluorescence-activated cell sorting |
| Q80093371 | Isolation of skin-derived precursors (SKPs) and differentiation and enrichment of their Schwann cell progeny |
| Q38303093 | Isolation of specific RNA-binding proteins using the streptomycin-binding RNA aptamer |
| Q38791134 | Isolation of the protein and RNA content of active sites of transcription from mammalian cells |
| Q89475574 | Isolation of tissue-resident vascular endothelial stem cells from mouse liver |
| Q87078298 | Isolation of ubiquitinated substrates by tandem affinity purification of E3 ligase-polyubiquitin-binding domain fusions (ligase traps) |
| Q41273631 | Isolation, characterization and propagation of mitotically active germ cells from adult mouse and human ovaries |
| Q34145070 | Isolation, cultivation and characterization of adult murine prostate stem cells |
| Q53781951 | Isolation, culture and characterization of primary mouse RPE cells. |
| Q34037254 | Isolation, culture and evaluation of multilineage-differentiating stress-enduring (Muse) cells |
| Q34001106 | Isolation, culture and genetic manipulation of mouse pancreatic ductal cells |
| Q33760945 | Isolation, differentiation and characterization of vascular cells derived from human embryonic stem cells |
| Q51032511 | Isothermal titration calorimetry to determine association constants for high-affinity ligands. |
| Q30157066 | Isotope labeling methods for studies of excited protein states by relaxation dispersion NMR spectroscopy |
| Q30360846 | Isotope labeling strategies for the study of high-molecular-weight proteins by solution NMR spectroscopy. |
| Q60428613 | Isotope-dilution mass spectrometry for exact quantification of noncanonical DNA nucleosides |
| Q28298658 | Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes |
| Q100526347 | Jointly defining cell types from multiple single-cell datasets using LIGER |
| Q48549696 | Juxtacellular recording and morphological identification of single neurons in freely moving rats. |
| Q42235425 | Kinetic flux profiling for quantitation of cellular metabolic fluxes |
| Q80286009 | Kinetic resolution of 4-chloro-2-(1-hydroxyalkyl)pyridines using Pseudomonas cepacia lipase |
| Q36588007 | Knockout and pullout recombineering for naturally transformable Burkholderia thailandensis and Burkholderia pseudomallei |
| Q33331328 | L-Measure: a web-accessible tool for the analysis, comparison and search of digital reconstructions of neuronal morphologies |
| Q51033616 | L-proline-catalyzed enantioselective one-pot cross-Mannich reaction of aldehydes. |
| Q34414344 | LOTOS-based two-photon calcium imaging of dendritic spines in vivo |
| Q47676759 | Lab-scale production of anhydrous diazomethane using membrane separation technology. |
| Q101237627 | Label-free capacitive assaying of biomarkers for molecular diagnostics |
| Q31062688 | Label-free quantification in ion mobility-enhanced data-independent acquisition proteomics. |
| Q36181091 | Labeling cellular structures in vivo using confined primed conversion of photoconvertible fluorescent proteins |
| Q40159187 | Labeling of active proteases in fresh-frozen tissues by topical application of quenched activity-based probes |
| Q80092963 | Labeling of aliphatic carboxylic acids using [11C]carbon monoxide |
| Q46090244 | Labeling of biotin antibodies with horseradish peroxidase using cyanuric chloride |
| Q48913327 | Labeling of immune cells for in vivo imaging using magnetofluorescent nanoparticles. |
| Q48196693 | Labeling proteins on live mammalian cells using click chemistry |
| Q44448398 | Labeling, detection and identification of newly synthesized proteomes with bioorthogonal non-canonical amino-acid tagging |
| Q39623747 | Laboratory breeding of the short-lived annual killifish Nothobranchius furzeri |
| Q34965860 | Laboratory procedures to generate viral metagenomes |
| Q47347820 | Langmuir-Blodgett nanotemplates for protein crystallography |
| Q86822779 | Large-area molecular patterning with polymer pen lithography |
| Q86386086 | Large-scale and chromatography-free synthesis of an octameric quinoline-based aromatic amide helical foldamer |
| Q45945840 | Large-scale engineered synthesis of BaTiO₃ nanoparticles using low-temperature bioinspired principles. |
| Q34358449 | Large-scale gene function analysis with the PANTHER classification system |
| Q33280773 | Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method |
| Q36496727 | Large-scale identification of ubiquitination sites by mass spectrometry |
| Q40467892 | Large-scale production and isolation of Candida biofilm extracellular matrix. |
| Q47957141 | Large-scale production of Plasmodium falciparum gametocytes for malaria drug discovery |
| Q57798900 | Large-scale reconstruction of cell lineages using single-cell readout of transcriptomes and CRISPR-Cas9 barcodes by scGESTALT |
| Q97587579 | Large-scale site-specific mapping of the O-GalNAc glycoproteome |
| Q36181086 | Laser microdissection of tomato fruit cell and tissue types for transcriptome profiling. |
| Q60673340 | Laser-capture microdissection |
| Q35020040 | Laser-induced choroidal neovascularization model to study age-related macular degeneration in mice. |
| Q43030514 | Leishmania parasite detection and quantification using PCR-ELISA. |
| Q63362880 | Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins |
| Q35975143 | Library design and screening protocol for artificial metalloenzymes based on the biotin-streptavidin technology |
| Q35195373 | Library preparation for highly accurate population sequencing of RNA viruses |
| Q90894118 | Life cycle maintenance and drug-sensitivity assays for early drug discovery in Schistosoma mansoni |
| Q98568595 | Ligand binding free-energy calculations with funnel metadynamics |
| Q46034933 | Ligand-based receptor identification on living cells and tissues using TRICEPS. |
| Q100491034 | Light-induced synthesis of protein conjugates and its application in photoradiosynthesis of 89Zr-radiolabeled monoclonal antibodies |
| Q112723912 | Lineage barcoding in mice with homing CRISPR |
| Q33947883 | Linear amplification for deep sequencing |
| Q33936190 | Lipid bilayer preparations of membrane proteins for oriented and magic-angle spinning solid-state NMR samples |
| Q35182231 | Liposome display for in vitro selection and evolution of membrane proteins. |
| Q80285988 | Liposome polymerase chain reaction assay for the sub-attomolar detection of cholera toxin and botulinum neurotoxin type A |
| Q28686521 | Liquid chromatography quadrupole time-of-flight mass spectrometry characterization of metabolites guided by the METLIN database |
| Q35094077 | Live imaging and quantitative analysis of gastrulation in mouse embryos using light-sheet microscopy and 3D tracking tools |
| Q35763111 | Live imaging of Tribolium castaneum embryonic development using light-sheet-based fluorescence microscopy |
| Q48284157 | Live imaging of axonal transport in Drosophila pupal brain explants. |
| Q50239529 | Live imaging of cellular dynamics during Caenorhabditis elegans postembryonic development. |
| Q51969779 | Live imaging of epidermal morphogenesis during the development of the adult abdominal epidermis of Drosophila. |
| Q57804871 | Live imaging of stem cells in the germarium of the Drosophila ovary using a reusable gas-permeable imaging chamber |
| Q46850558 | Live imaging of synapse development and measuring protein dynamics using two-color fluorescence recovery after photo-bleaching at Drosophila synapses |
| Q36942740 | Live visualization of protein synthesis in axonal growth cones by microinjection of photoconvertible Kaede into Xenopus embryos |
| Q33280770 | Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D. |
| Q62577372 | Live-cell assay to detect antigen-specific CD4+ T-cell responses by CD154 expression |
| Q47705920 | Live-cell confocal microscopy and quantitative 4D image analysis of anchor-cell invasion through the basement membrane in Caenorhabditis elegans |
| Q44407514 | Live-cell imaging of receptors around postsynaptic membranes |
| Q47266410 | Live-cell measurements of kinase activity in single cells using translocation reporters |
| Q33280941 | Localized transfection with magnetic beads coated with PCR products and other nucleic acids |
| Q58054176 | Locating potential enhancer elements by comparative genomics using the EEL software |
| Q29547300 | Locating proteins in the cell using TargetP, SignalP and related tools |
| Q80482511 | Locked nucleic acid-based in situ detection of microRNAs in mouse tissue sections |
| Q33280983 | Long PCR Product Sequencing (LoPPS): a shotgun-based approach to sequence long PCR products |
| Q56986042 | Long PCR amplification of the entire mitochondrial genome from individual helminths for direct sequencing |
| Q36931684 | Long term non-invasive imaging of embryonic stem cells using reporter genes |
| Q36327941 | Long-read ChIA-PET for base-pair-resolution mapping of haplotype-specific chromatin interactions |
| Q88562679 | Long-read sequencing data analysis for yeasts |
| Q112757037 | Long-term culture, genetic manipulation and xenotransplantation of human normal and breast cancer organoids |
| Q92503905 | Long-term ex vivo expansion of mouse hematopoietic stem cells |
| Q89637910 | Long-term in vivo imaging of Drosophila larvae |
| Q48218820 | Long-term live imaging of neuronal circuits in organotypic hippocampal slice cultures |
| Q35370665 | Long-term microfluidic cultures of myotube microarrays for high-throughput focal stimulation |
| Q80093352 | Long-term primary culture of secretory cells of Bothrops jararaca venom gland for venom production in vitro |
| Q33484154 | Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window |
| Q38834610 | Looking to the future following 10 years of induced pluripotent stem cell technologies |
| Q28278632 | Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products |
| Q53090290 | Low-bias, strand-specific transcriptome Illumina sequencing by on-flowcell reverse transcription (FRT-seq). |
| Q45873650 | Lung-directed gene therapy in mice using the nonviral Sleeping Beauty transposon system |
| Q42634194 | Lysine-specific chemical cross-linking of protein complexes and identification of cross-linking sites using LC-MS/MS and the xQuest/xProphet software pipeline |
| Q64108792 | MARS-seq2.0: an experimental and analytical pipeline for indexed sorting combined with single-cell RNA sequencing |
| Q38811005 | MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems. |
| Q91013991 | MOWChIP-seq for low-input and multiplexed profiling of genome-wide histone modifications |
| Q81017324 | MRI of postmortem specimens of endangered species for comparative brain anatomy |
| Q47416980 | MRI-guided stereotaxic brain surgery in the infant and adult common marmoset |
| Q92611083 | Machine learning workflows to estimate class probabilities for precision cancer diagnostics on DNA methylation microarray data |
| Q96610228 | Macrophage phagocytosis assay with reconstituted target particles |
| Q35888947 | Magnetic-based purification of untouched mouse germinal center B cells for ex vivo manipulation and biochemical analysis |
| Q90813200 | Magnetically guided virus stamping for the targeted infection of single cells or groups of cells |
| Q38796653 | Mammalian NET-seq analysis defines nascent RNA profiles and associated RNA processing genome-wide |
| Q92503885 | Manipulating gene translation in plants by CRISPR-Cas9-mediated genome editing of upstream open reading frames |
| Q92503897 | Manipulating the hydrophobicity of DNA as a universal strategy for visual biosensing |
| Q89948395 | Manual and automated Cu-mediated radiosynthesis of the PARP inhibitor [18F]olaparib |
| Q96952599 | Manual and automated preparation of single-stranded DNA libraries for the sequencing of DNA from ancient biological remains and other sources of highly degraded DNA |
| Q36148298 | Mapping 3D genome architecture through in situ DNase Hi-C |
| Q28305549 | Mapping Argonaute and conventional RNA-binding protein interactions with RNA at single-nucleotide resolution using HITS-CLIP and CIMS analysis |
| Q90754880 | Mapping RNA-chromatin interactions by sequencing with iMARGI |
| Q46558029 | Mapping genome-wide transcription-factor binding sites using DAP-seq. |
| Q57129445 | Mapping heterogeneity of cellular mechanics by multi-harmonic atomic force microscopy |
| Q35174434 | Mapping identifiers for the integration of genomic datasets with the R/Bioconductor package biomaRt |
| Q61456035 | Mapping in vivo target interaction profiles of covalent inhibitors using chemical proteomics with label-free quantification |
| Q112720600 | Mapping mechanical properties of biological materials via an add-on Brillouin module to confocal microscopes |
| Q34578862 | Mapping networks of physical interactions between genomic elements using 5C technology |
| Q102369570 | Mapping replication timing domains genome wide in single mammalian cells with single-cell DNA replication sequencing |
| Q54238731 | Mapping ribonucleotides in genomic DNA and exploring replication dynamics by polymerase usage sequencing (Pu-seq). |
| Q35104194 | Mapping the miRNA interactome by cross-linking ligation and sequencing of hybrids (CLASH) |
| Q47320143 | Mapping the small RNA interactome in bacteria using RIL-seq |
| Q42132254 | Marker selection for genetic case-control association studies |
| Q47839893 | Mass cytometry analysis of immune cells in the brain |
| Q51032344 | Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site-specific stable isotope tagging. |
| Q40740510 | Mass spectrometry of intact membrane protein complexes |
| Q83877174 | Mass spectrometry-based detection and quantification of plasma glycoproteins using selective reaction monitoring |
| Q92052807 | Mass spectrometry-based identification of MHC-bound peptides for immunopeptidomics |
| Q38610344 | Massively parallel and multiparameter titration of biochemical assays with droplet microfluidics |
| Q34220322 | Materials fabrication from Bombyx mori silk fibroin |
| Q51824096 | Measurement of activity of collagenolytic MMP and inhibitors of MMPs using radiolabeled collagen substrate. |
| Q51771312 | Measurement of auxin transport in Arabidopsis thaliana. |
| Q24648818 | Measurement of cardiac function using pressure-volume conductance catheter technique in mice and rats |
| Q46020962 | Measurement of cell proliferation by heavy water labeling. |
| Q81246971 | Measurement of chronic oxidative and inflammatory stress by quantification of isoketal/levuglandin gamma-ketoaldehyde protein adducts using liquid chromatography tandem mass spectrometry |
| Q41840629 | Measurement of cytochrome P450 and NADPH-cytochrome P450 reductase |
| Q89687006 | Measurement of differential chromatin interactions with absolute quantification of architecture (AQuA-HiChIP) |
| Q47949142 | Measurement of drug-target engagement in live cells by two-photon fluorescence anisotropy imaging |
| Q38972136 | Measurement of methylglyoxal by stable isotopic dilution analysis LC-MS/MS with corroborative prediction in physiological samples |
| Q64963161 | Measurement of phosphoinositides in the zebrafish Danio rerio. |
| Q43288464 | Measurement of proliferation and disappearance of rapid turnover cell populations in human studies using deuterium-labeled glucose. |
| Q33669995 | Measurement of protein farnesylation and geranylgeranylation in vitro, in cultured cells and in biopsies, and the effects of prenyl transferase inhibitors |
| Q80188302 | Measurement of reduced, oxidized and total ascorbate content in plants |
| Q39358745 | Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue |
| Q82491708 | Measurement of telomere length by the Southern blot analysis of terminal restriction fragment lengths |
| Q83977296 | Measurement of the mechanical behavior of yeast membrane sensors using single-molecule atomic force microscopy |
| Q47254146 | Measurement of the nucleation of atmospheric aerosol particles |
| Q46735087 | Measurement of very low rates of cell proliferation by heavy water labeling of DNA and gas chromatography/pyrolysis/isotope ratio-mass spectrometric analysis |
| Q34074267 | Measuring and interpreting point spread functions to determine confocal microscope resolution and ensure quality control |
| Q38819892 | Measuring antigen presentation in mouse brain endothelial cells ex vivo and in vitro |
| Q42844153 | Measuring behavioral and endocrine responses to novelty stress in adult zebrafish. |
| Q84857373 | Measuring bone blood supply in mice using fluorescent microspheres |
| Q33280954 | Measuring calcium signaling using genetically targetable fluorescent indicators |
| Q80643404 | Measuring distances in proteins by saturation-recovery EPR |
| Q46554003 | Measuring elastase, proteinase 3 and cathepsin G activities at the surface of human neutrophils with fluorescence resonance energy transfer substrates |
| Q37284264 | Measuring energy metabolism in cultured cells, including human pluripotent stem cells and differentiated cells. |
| Q48270648 | Measuring genetic interactions in human cells by RNAi and imaging |
| Q45404291 | Measuring herpes simplex virus thymidine kinase reporter gene expression in vitro |
| Q28248454 | Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data |
| Q33644552 | Measuring mRNA copy number in individual Escherichia coli cells using single-molecule fluorescent in situ hybridization |
| Q47351515 | Measuring mutation accumulation in single human adult stem cells by whole-genome sequencing of organoid cultures |
| Q36724988 | Measuring nanometer distances in nucleic acids using a sequence-independent nitroxide probe |
| Q63408536 | Measuring nanoscale diffusion dynamics in cellular membranes with super-resolution STED–FCS |
| Q47601095 | Measuring protein structural changes on a proteome-wide scale using limited proteolysis-coupled mass spectrometry |
| Q34166016 | Measuring single-cell gene expression dynamics in bacteria using fluorescence time-lapse microscopy. |
| Q84731692 | Measuring substrate binding and affinity of purified membrane transport proteins using the scintillation proximity assay |
| Q48286456 | Measuring surface potential changes on leaves. |
| Q35234062 | Measuring the activity of protein variants on a large scale using deep mutational scanning |
| Q52578170 | Measuring the optokinetic response of zebrafish larvae. |
| Q49218366 | Mechanical fixation techniques for processing and orienting delicate samples, such as the root of Arabidopsis thaliana, for light or electron microscopy. |
| Q46326458 | Mechanical isolation, and measurement of force and myoplasmic free [Ca2+] in fully intact single skeletal muscle fibers |
| Q47784656 | Meganuclease-assisted generation of stable transgenics in the sea anemone Nematostella vectensis |
| Q36619224 | MeltMADGE for mutation scanning of specific genes in population studies |
| Q46754390 | Membrane reconstitution of ABC transporters and assays of translocator function. |
| Q52784076 | Merging allylic C-H bond activation and C-C bond cleavage en route to the formation of a quaternary carbon stereocenter in acyclic systems. |
| Q43416808 | Merging high-quality biochemical fractionation with a refined flow cytometry approach to monitor nucleocytoplasmic protein expression throughout the unperturbed mammalian cell cycle |
| Q30496172 | Mesoscopic hydrogel molding to control the 3D geometry of bioartificial muscle tissues |
| Q34161462 | Meta-analysis of untargeted metabolomic data from multiple profiling experiments |
| Q57638693 | MetaNetwork: a computational protocol for the genetic study of metabolic networks |
| Q51009449 | Metabolic expression of thiol-derivatized sialic acids on the cell surface and their quantitative estimation by flow cytometry. |
| Q43576814 | Metabolic labeling of glycans with azido sugars and subsequent glycan-profiling and visualization via Staudinger ligation |
| Q34714204 | Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts |
| Q33974831 | Metabolite extraction from suspension-cultured mammalian cells for global metabolite profiling |
| Q38903248 | Metabolomics and lipidomics using traveling-wave ion mobility mass spectrometry. |
| Q125345322 | Metagenome analysis using the Kraken software suite |
| Q55096031 | Metal- and additive-free photoinduced borylation of haloarenes. |
| Q51033603 | Metal-chelate affinity precipitation of proteins using responsive polymers. |
| Q89687018 | Metal-free photocatalysts for the oxidation of non-activated alcohols and the oxygenation of tertiary amines performed in air or oxygen |
| Q91785787 | Metal-isotope-tagged monoclonal antibodies for high-dimensional mass cytometry |
| Q80093206 | Methods for MAG3 conjugation and 99mTc radiolabeling of biomolecules |
| Q30436361 | Methods for analyzing peptides and proteins on a chromatographic timescale by electron-transfer dissociation mass spectrometry |
| Q80487867 | Methods for detection, isolation and culture of mouse and human invariant NKT cells |
| Q37001313 | Methods for generating and colonizing gnotobiotic zebrafish |
| Q51635981 | Methods for isolation of marine-derived endophytic fungi and their bioactive secondary products. |
| Q46260757 | Methods for isolation, purification and structural elucidation of bioactive secondary metabolites from marine invertebrates |
| Q52700343 | Methods for quantifying simple gravity sensing in Drosophila melanogaster. |
| Q39973206 | Methods for simultaneous measurement of apoptosis and cell surface phenotype of epithelial cells in effusions by flow cytometry |
| Q33305814 | Methods of using click chemistry in the discovery of enzyme inhibitors |
| Q46814636 | Methods, setup and safe handling for anhydrous hydrogen fluoride cleavage in Boc solid-phase peptide synthesis |
| Q35563521 | MethylC-seq library preparation for base-resolution whole-genome bisulfite sequencing |
| Q36047142 | Methylation-assisted bisulfite sequencing to simultaneously map 5fC and 5caC on a genome-wide scale for DNA demethylation analysis |
| Q57390838 | Methylation-sensitive high-resolution melting |
| Q80805160 | Methylation-sensitive single-nucleotide primer extension (Ms-SNuPE) for quantitative measurement of DNA methylation |
| Q28296362 | Methylation-specific digital karyotyping |
| Q34189000 | Methylome analysis using MeDIP-seq with low DNA concentrations |
| Q38414412 | Micro- and nanopatterning by lithographically controlled wetting |
| Q44890469 | MicroRNA detection by northern blotting using locked nucleic acid probes |
| Q34767925 | MicroRNA expression profiling using microarrays |
| Q50560887 | MicroRNA-based conversion of human fibroblasts into striatal medium spiny neurons. |
| Q39409455 | Microarray analysis of copy number variation in single cells. |
| Q45248719 | Microcomputed tomography-based structural analysis of various bone tissue regeneration models. |
| Q80093174 | Microcontact printing of axon guidance molecules for generation of graded patterns |
| Q92887110 | Microfabricated blood vessels for modeling the vascular transport barrier |
| Q90724261 | Microfabrication of AngioChip, a biodegradable polymer scaffold with microfluidic vasculature |
| Q45924666 | Microfabrication of human organs-on-chips. |
| Q33669086 | Microfluidic assay for simultaneous culture of multiple cell types on surfaces or within hydrogels |
| Q46771334 | Microfluidic culture platform for neuroscience research |
| Q36969448 | Microfluidic differential immunocapture biochip for specific leukocyte counting |
| Q33762072 | Microfluidic immobilization of physiologically active Caenorhabditis elegans |
| Q44589111 | Microfluidic mixer designed for performing single-molecule kinetics with confocal detection on timescales from milliseconds to minutes |
| Q91945569 | Microfluidic reprogramming to pluripotency of human somatic cells |
| Q36853922 | Microfluidic single-cell real-time PCR for comparative analysis of gene expression patterns |
| Q44005166 | Microfluidic trap array for massively parallel imaging of Drosophila embryos |
| Q34263808 | Microfluidic, marker-free isolation of circulating tumor cells from blood samples |
| Q37388573 | Microfluidic-assisted analysis of replicating DNA molecules |
| Q34665874 | Microinjection of mRNA or morpholinos for reverse genetic analysis in the starlet sea anemone, Nematostella vectensis |
| Q35156816 | Microinjection of membrane-impermeable molecules into single neural stem cells in brain tissue |
| Q33980347 | Microneedle-based analysis of the micromechanics of the metaphase spindle assembled in Xenopus laevis egg extracts |
| Q51032403 | Micropatterned cell cultures on elastic membranes as an in vitro model of myocardium. |
| Q38818466 | Micropatterned coculture of primary human hepatocytes and supportive cells for the study of hepatotropic pathogens. |
| Q33280966 | Micropatterning of living cells by laser-guided direct writing: application to fabrication of hepatic-endothelial sinusoid-like structures |
| Q33470204 | Micropicking of nannofossils in preparation for analysis by secondary ion mass spectrometry |
| Q91272612 | Micropipette force sensors for in vivo force measurements on single cells and multicellular microorganisms |
| Q80078238 | Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method |
| Q52601813 | Microtiter susceptibility testing of microbes growing on peg lids: a miniaturized biofilm model for high-throughput screening. |
| Q57540648 | Microwave-assisted preparation of the quorum-sensing molecule 2-heptyl-3-hydroxy-4(1H)-quinolone and structurally related analogs |
| Q43199678 | Microwave-assisted synthesis of triple-helical, collagen-mimetic lipopeptides |
| Q83131498 | Miniaturized parallel screens to identify chromatographic steps required for recombinant protein purification |
| Q101237625 | Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results |
| Q93184599 | Mitigating head motion artifact in functional connectivity MRI |
| Q80093517 | Mixed-culture assays for analyzing neuronal synapse formation |
| Q60185086 | Mixed-species RNA-seq for elucidation of non-cell-autonomous control of gene transcription |
| Q48860089 | Modeling Pseudomonas aeruginosa pathogenesis in plant hosts. |
| Q45858962 | Modeling and correction of structural variations in patient-derived iPSCs using CRISPR/Cas9. |
| Q46622702 | Modeling and docking of antibody structures with Rosetta. |
| Q47794968 | Modeling human listeriosis in natural and genetically engineered animals |
| Q35629661 | Models of oral and vaginal candidiasis based on in vitro reconstituted human epithelia |
| Q60106617 | Modular approach for resolving and mapping complex neural and other cellular structures and their associated deformation fields in three dimensions |
| Q36351749 | Modular low-light microscope for imaging cellular bioluminescence and radioluminescence. |
| Q33548644 | Modular system for the construction of zinc-finger libraries and proteins |
| Q40149997 | Molecular PET imaging of HSV1-tk reporter gene expression using [18F]FEAU. |
| Q35077743 | Molecular beacon-enabled purification of living cells by targeting cell type-specific mRNAs. |
| Q35738569 | Molecular characterization of neuronal cell types based on patterns of projection with Retro-TRAP. |
| Q40133096 | Molecular mechanisms of protein aggregation from global fitting of kinetic models. |
| Q35121508 | Monitoring apoptosis and neuronal degeneration by real-time detection of phosphatidylserine externalization using a polarity-sensitive indicator of viability and apoptosis. |
| Q120691729 | Monitoring cell membrane recycling dynamics of proteins using whole-cell fluorescence recovery after photobleaching of pH-sensitive genetic tags |
| Q47994215 | Monitoring detergent-mediated solubilization and reconstitution of lipid membranes by isothermal titration calorimetry |
| Q93382511 | Monitoring early S-phase origin firing and replication fork movement by sequencing nascent DNA from synchronized cells |
| Q112709146 | Monitoring genome-wide replication fork directionality by Okazaki fragment sequencing in mammalian cells |
| Q53057031 | Monitoring in vivo reversible cysteine oxidation in proteins using ICAT and mass spectrometry. |
| Q50146016 | Monitoring intracellular nanomolar calcium using fluorescence lifetime imaging. |
| Q36641339 | Monitoring local synaptic activity with astrocytic patch pipettes. |
| Q28248444 | Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester |
| Q112713111 | Monitoring mammalian mitochondrial translation with MitoRiboSeq |
| Q50660744 | Monitoring of cell therapy and assessment of cardiac function using magnetic resonance imaging in a mouse model of myocardial infarction. |
| Q33484162 | Monitoring peptidase activities in complex proteomes by MALDI-TOF mass spectrometry |
| Q43195607 | Monitoring protein conformational changes and dynamics using stable-isotope labeling and mass spectrometry |
| Q52894941 | Monitoring protein expression in whole-cell extracts by targeted label- and standard-free LC-MS/MS. |
| Q46754388 | Monitoring structural changes in nucleic acids with single residue spatial and millisecond time resolution by quantitative hydroxyl radical footprinting |
| Q38789272 | Monitoring the progression of cell death and the disassembly of dying cells by flow cytometry. |
| Q41490747 | Monitoring the spatiotemporal dynamics of proteins at replication forks and in assembled chromatin using isolation of proteins on nascent DNA. |
| Q53021044 | Monolayer culturing and cloning of human pluripotent stem cells on laminin-521-based matrices under xeno-free and chemically defined conditions. |
| Q50660750 | Monoplex/multiplex linear-after-the-exponential-PCR assays combined with PrimeSafe and Dilute-'N'-Go sequencing. |
| Q28296351 | Morris water maze: procedures for assessing spatial and related forms of learning and memory |
| Q38301154 | Mos1-mediated insertional mutagenesis in Caenorhabditis elegans |
| Q46941950 | Mosher ester analysis for the determination of absolute configuration of stereogenic (chiral) carbinol carbons |
| Q30826166 | Motif-based analysis of large nucleotide data sets using MEME-ChIP. |
| Q47174587 | Mouse closed head injury model induced by a weight-drop device |
| Q84261553 | Mouse corneal lymphangiogenesis model |
| Q48769089 | Mouse embryonic stem cell culture for generation of three-dimensional retinal and cortical tissues. |
| Q34342112 | Mouse intragastric infusion (iG) model. |
| Q37210744 | Mouse model of chronic and binge ethanol feeding (the NIAAA model) |
| Q36900988 | Mouse model of oropharyngeal candidiasis |
| Q92611070 | Multi-contact 4C: long-molecule sequencing of complex proximity ligation products to uncover local cooperative and competitive chromatin topologies |
| Q35542147 | Multi-stage chemical carcinogenesis in mouse skin: fundamentals and applications |
| Q50606681 | Multichannel wholemount fluorescent and fluorescent/chromogenic in situ hybridization in Xenopus embryos. |
| Q33940052 | Multicolor multicycle molecular profiling with quantum dots for single-cell analysis |
| Q93040380 | Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS |
| Q46098216 | Multifunctional ligands based on dihydrolipoic acid and polyethylene glycol to promote biocompatibility of quantum dots |
| Q58231187 | Multifunctional protein labeling via enzymatic N-terminal tagging and elaboration by click chemistry |
| Q114647743 | Multigenerational laboratory culture of pelagic ctenophores and CRISPR–Cas9 genome editing in the lobate Mnemiopsis leidyi |
| Q112725851 | Multimodal detection of protein isoforms and nucleic acids from mouse pre-implantation embryos |
| Q47299611 | Multimodal profiling of single-cell morphology, electrophysiology, and gene expression using Patch-seq. |
| Q92669575 | Multiparameter screening method for developing optimized red-fluorescent proteins |
| Q33305815 | Multiparametric analysis of cells with different mitochondrial membrane potential during apoptosis by polychromatic flow cytometry |
| Q40054042 | Multiparametric characterization of rare HIV-infected cells using an RNA-flow FISH technique |
| Q48315684 | Multiparametric high-resolution imaging of native proteins by force-distance curve-based AFM. |
| Q48515447 | Multiple neuroanatomical tract-tracing using fluorescent Alexa Fluor conjugates of cholera toxin subunit B in rats. |
| Q30145570 | Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples. |
| Q80093335 | Multiplex PCR-based reverse line blot hybridization assay (mPCR/RLB)--a practical epidemiological and diagnostic tool |
| Q47797623 | Multiplex amplification of ancient DNA. |
| Q51758332 | Multiplex peptide stable isotope dimethyl labeling for quantitative proteomics. |
| Q38463111 | Multiplex-fluorescence in situ hybridization for chromosome karyotyping |
| Q57273493 | Multiplex-terminal restriction fragment length polymorphism |
| Q58023456 | Multiplexed absolute quantification for proteomics using concatenated signature peptides encoded by QconCAT genes |
| Q34066566 | Multiplexed array-based and in-solution genomic enrichment for flexible and cost-effective targeted next-generation sequencing |
| Q34587822 | Multiplexed chromosome conformation capture sequencing for rapid genome-scale high-resolution detection of long-range chromatin interactions |
| Q36112717 | Multiplexed high-content analysis of mitochondrial morphofunction using live-cell microscopy |
| Q33730670 | Multiplexed immunophenotyping of human antigen-presenting cells in whole blood by polychromatic flow cytometry |
| Q45952339 | Multiplexed locus-specific analysis of DNA methylation in single cells. |
| Q30956067 | Multiplexed peptide analysis using data-independent acquisition and Skyline |
| Q91387291 | Multiplexed profiling of RNA and protein expression signatures in individual cells using flow or mass cytometry |
| Q50091611 | Multiplexed proteome analysis with neutron-encoded stable isotope labeling in cells and mice. |
| Q112303538 | Multiplexed single-cell proteomics using SCoPE2 |
| Q44399629 | Multiscale analysis of genome-wide replication timing profiles using a wavelet-based signal-processing algorithm. |
| Q33848889 | Multisubstrate-compatible ELISA procedures for rapid and high-sensitivity immunoassays |
| Q47841405 | Murine chronic lymph node window for longitudinal intravital lymph node imaging |
| Q49218396 | Mussel micronucleus cytome assay. |
| Q80093419 | Mutation screening using fluorescence multiplex denaturing gradient gel electrophoresis (FMD): detecting mutations in the BRCA1 gene |
| Q36507255 | Myelination of rodent hippocampal neurons in culture |
| Q37606101 | Myocardial infarction and intramyocardial injection models in swine |
| Q50930923 | N-formylation and N-methylation of amines using metal-free N-heterocyclic carbene catalysts and CO2 as carbon source. |
| Q98196901 | NAD tagSeq for transcriptome-wide identification and characterization of NAD+-capped RNAs |
| Q30390240 | NMR protein structure determination in living E. coli cells using nonlinear sampling |
| Q57057960 | NMR-based metabolomic analysis of plants |
| Q48590571 | NMR: prediction of molecular alignment from structure using the PALES software. |
| Q34615376 | NMR: prediction of protein flexibility |
| Q92615467 | Nanoscale chemical imaging using tip-enhanced Raman spectroscopy |
| Q58115625 | Nanoscale fiber-optic force sensors for mechanical probing at the molecular and cellular level |
| Q90836388 | Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology |
| Q33303181 | Nanostructure analysis using spatially modulated illumination microscopy |
| Q33361562 | Nanostructure-initiator mass spectrometry: a protocol for preparing and applying NIMS surfaces for high-sensitivity mass analysis |
| Q91272689 | Native internally calibrated chromatin immunoprecipitation for quantitative studies of histone post-translational modifications |
| Q38787353 | Near-IR photoactivation using mesoporous silica-coated NaYF4:Yb,Er/Tm upconversion nanoparticles |
| Q52560087 | Near-infrared light-controlled systems for gene transcription regulation, protein targeting and spectral multiplexing. |
| Q51032399 | Negative detection of biomolecules separated in polyacrylamide electrophoresis gels. |
| Q51868865 | Network Analysis Tools: from biological networks to clusters and pathways. |
| Q33508084 | Network visualization and analysis of gene expression data using BioLayout Express(3D). |
| Q30945958 | NetworkAnalyst for statistical, visual and network-based meta-analysis of gene expression data |
| Q81464910 | Neurologic assessment of somatosensory dysfunction following an experimental rodent model of cerebral ischemia |
| Q45296763 | Neutral desorption sampling of biological surfaces for rapid chemical characterization by extractive electrospray ionization mass spectrometry |
| Q30047632 | Neutral red uptake assay for the estimation of cell viability/cytotoxicity |
| Q33787807 | Next-generation diagnostics and disease-gene discovery with the Exomiser |
| Q56773901 | Nicking enzyme–based internal labeling of DNA at multiple loci |
| Q42872955 | Nitroreductase-mediated cell/tissue ablation in zebrafish: a spatially and temporally controlled ablation method with applications in developmental and regeneration studies. |
| Q33280960 | Non-SELEX: selection of aptamers without intermediate amplification of candidate oligonucleotides |
| Q51032317 | Non-invasive measurement of bioelectric currents with a vibrating probe. |
| Q36982931 | Noncatalytic reaction of isonitriles and carboxylic acids en route to amide-type linkages |
| Q103029551 | Nondestructive production of exosomes loaded with ultrathin palladium nanosheets for targeted bio-orthogonal catalysis |
| Q93066024 | Nongenetic optical neuromodulation with silicon-based materials |
| Q90724248 | Noninvasive 40-Hz light flicker to recruit microglia and reduce amyloid beta load |
| Q33361559 | Noninvasive high-resolution in vivo imaging of cell biology in the anterior chamber of the mouse eye |
| Q33529609 | Nonlinear least-squares data fitting in Excel spreadsheets. |
| Q80093221 | Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol |
| Q46260761 | Nonradioactive enzymatic assay for plasma and serum vitamin B(6). |
| Q38721727 | Nonradioactive quantification of autophagic protein degradation with L-azidohomoalanine labeling. |
| Q38657588 | Nontargeted virus sequence discovery pipeline and virus clustering for metagenomic data |
| Q91908217 | Nonviral ultrasound-mediated gene delivery in small and large animal models |
| Q80092873 | Normal human keratinocyte assay to predict the human irritancy response |
| Q34918147 | Northern blot analysis for detection and quantification of RNA in pancreatic cancer cells and tissues |
| Q112586526 | Nuclei isolation of multiple brain cell types for omics interrogation |
| Q90754874 | Nucleotide resolution profiling of m7G tRNA modification by TRAC-Seq |
| Q47903507 | O2-controllable hydrogels for studying cellular responses to hypoxic gradients in three dimensions in vitro and in vivo |
| Q112584907 | OCTAD: an open workspace for virtually screening therapeutics targeting precise cancer patient groups using gene expression features |
| Q45974781 | OLIG gene targeting in human pluripotent stem cells for motor neuron and oligodendrocyte differentiation. |
| Q48406875 | Object recognition in rats and mice: a one-trial non-matching-to-sample learning task to study 'recognition memory'. |
| Q44216716 | Object recognition test in mice. |
| Q35145325 | Obtaining genomes from uncultivated environmental microorganisms using FACS-based single-cell genomics |
| Q41474648 | Obtaining information about protein secondary structures in aqueous solution using Fourier transform IR spectroscopy |
| Q46309294 | Off-wafer fabrication and surface modification of asymmetric 3D SU-8 microparticles |
| Q42787017 | Olefin cross-metathesis-based approaches to furans: procedures for the preparation of di- and trisubstituted variants |
| Q33508083 | Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays |
| Q92611056 | On-chip 3D neuromuscular model for drug screening and precision medicine in neuromuscular disease |
| Q33902543 | On-chip human microvasculature assay for visualization and quantification of tumor cell extravasation dynamics |
| Q51415377 | On-chip microfluidic production of cell-sized liposomes. |
| Q47721283 | On-demand synthesis of organozinc halides under continuous flow conditions. |
| Q37569958 | On-wire lithography: synthesis, encoding and biological applications |
| Q50991243 | One-hour proteome analysis in yeast. |
| Q41579532 | One-pot chemical synthesis of small ubiquitin-like modifier protein-peptide conjugates using bis(2-sulfanylethyl)amido peptide latent thioester surrogates |
| Q24655069 | One-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities |
| Q28817698 | One-step (18)F labeling of biomolecules using organotrifluoroborates |
| Q87411143 | One-step (18)F-labeling of peptides for positron emission tomography imaging using the SiFA methodology |
| Q40150148 | One-step analysis of protein complexes in microliters of cell lysate using indirect immunolabeling & fluorescence cross-correlation spectroscopy |
| Q90663973 | One-step site-specific antibody fragment auto-conjugation using SNAP-tag technology |
| Q53629089 | One-step targeted gene deletion in Candida albicans haploids. |
| Q42398799 | One-way PCR-based mapping of a replication initiation point (RIP). |
| Q57217248 | Optical measurements to determine the thickness of calcite crystals and the mass of thin carbonate particles such as coccoliths |
| Q34061369 | Optical sensors for measuring dynamic changes of cytosolic metabolite levels in yeast. |
| Q34228523 | Optical sensors for monitoring dynamic changes of intracellular metabolite levels in mammalian cells |
| Q40109230 | Optimization of drug combinations using Feedback System Control |
| Q33552925 | Optimization of immunoprecipitation-western blot analysis in detecting GW182-associated components of GW/P bodies |
| Q87476406 | Optimization of nucleophilic ¹⁸F radiofluorinations using a microfluidic reaction approach |
| Q35612782 | Optimized DNA extraction and metagenomic sequencing of airborne microbial communities |
| Q30586225 | Optimized RNA ISH, RNA FISH and protein-RNA double labeling (IF/FISH) in Drosophila ovaries |
| Q43615088 | Optimized axolotl (Ambystoma mexicanum) husbandry, breeding, metamorphosis, transgenesis and tamoxifen-mediated recombination |
| Q48145689 | Optimized labeling of membrane proteins for applications to super-resolution imaging in confined cellular environments using monomeric streptavidin. |
| Q47657766 | Optimized mouse ES cell culture system by suspension growth in a fully defined medium |
| Q84513055 | Optimized multiparametric immunophenotyping of umbilical cord blood cells by flow cytometry |
| Q40063172 | Optimized retroviral transduction of mouse T cells for in vivo assessment of gene function |
| Q35662039 | Optimized sample preparation for single-molecule localization-based superresolution microscopy in yeast |
| Q34102272 | Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures |
| Q30543434 | Optogenetic manipulation of neural circuits and behavior in Drosophila larvae |
| Q60056610 | Organelle isolation: functional mitochondria from mouse liver, muscle and cultured filroblasts |
| Q39035547 | Organoid culture systems for prostate epithelial and cancer tissue. |
| Q97636492 | Organotypic culture assays for murine and human primary and metastatic-site tumors |
| Q53090038 | Orthotopic mouse liver transplantation to study liver biology and allograft tolerance. |
| Q37359724 | Orthotopic mouse lung transplantation as experimental methodology to study transplant and tumor biology |
| Q39470722 | Overexpressing human membrane proteins in stably transfected and clonal human embryonic kidney 293S cells |
| Q37678426 | Oxidative aliphatic C-H fluorination with manganese catalysts and fluoride ion |
| Q41938716 | Oxidative bisulfite sequencing of 5-methylcytosine and 5-hydroxymethylcytosine |
| Q34197585 | Oxime-based linker libraries as a general approach for the rapid generation and screening of multidentate inhibitors |
| Q46421359 | PCR-amplification of GC-rich regions: 'slowdown PCR'. |
| Q80092960 | PCR-based accurate synthesis of long DNA sequences |
| Q46388232 | PCR-based gene targeting in Candida albicans. |
| Q60015369 | PEG-4MAL hydrogels for human organoid generation, culture, and in vivo delivery |
| Q80093243 | PEGylation of native disulfide bonds in proteins |
| Q45405582 | PET imaging of herpes simplex virus type 1 thymidine kinase (HSV1-tk) or mutant HSV1-sr39tk reporter gene expression in mice and humans using [18F]FHBG. |
| Q48920325 | Paired-recordings from synaptically coupled cortical and hippocampal neurons in acute and cultured brain slices. |
| Q35141765 | Palladium-based mass tag cell barcoding with a doublet-filtering scheme and single-cell deconvolution algorithm |
| Q48129281 | Palmitoylated proteins: purification and identification |
| Q36526004 | Pancreatic cell tracing, lineage tagging and targeted genetic manipulations in multiple cell types using pancreatic ductal infusion of adeno-associated viral vectors and/or cell-tagging dyes |
| Q60634938 | Parallel detection of antigen-specific T cell responses by combinatorial encoding of MHC multimers |
| Q33280990 | Parallel synthesis of peptide libraries using microwave irradiation |
| Q33319840 | Parallel tagged sequencing on the 454 platform |
| Q48245916 | Particle generation, functionalization and sortase A-mediated modification with targeting of single-chain antibodies for diagnostic and therapeutic use. |
| Q36607574 | Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions |
| Q80285957 | Passive induction of experimental allergic encephalomyelitis |
| Q83146433 | Patch clamp recording from enteric neurons in situ |
| Q48175426 | Patch-clamp recording from mossy fiber terminals in hippocampal slices. |
| Q38654537 | Patch-clamp technique to characterize ion channels in enlarged individual endolysosomes |
| Q50142963 | Patent searches as a complement to literature searches in the life sciences--a 'how-to' tutorial. |
| Q63484960 | Pathway enrichment analysis and visualization of omics data using g:Profiler, GSEA, Cytoscape and EnrichmentMap |
| Q34725582 | Pd-catalyzed Suzuki-Miyaura reactions of aryl halides using bulky biarylmonophosphine ligands |
| Q34714214 | Pd-catalyzed amination reactions of aryl halides using bulky biarylmonophosphine ligands. |
| Q114109152 | Peat-based gnotobiotic plant growth systems for Arabidopsis microbiome research |
| Q33286526 | Peptide arrays on cellulose support: SPOT synthesis, a time and cost efficient method for synthesis of large numbers of peptides in a parallel and addressable fashion |
| Q40050946 | Peptide microarrays for high-throughput studies of Ser/Thr phosphatases |
| Q123558300 | Perfusion and endothelialization of engineered tissues with patterned vascular networks |
| Q53539328 | Perfusion decellularization of whole organs. |
| Q62168753 | Peterson olefination from α-silyl aldehydes |
| Q56888614 | PhIP-Seq characterization of serum antibodies using oligonucleotide-encoded peptidomes |
| Q102075536 | Phage-assisted continuous and non-continuous evolution |
| Q50780661 | Pharmacological manipulation of blood and lymphatic vascularization in ex vivo-cultured mouse embryos. |
| Q33847390 | Phenotyping sensory nerve endings in vitro in the mouse |
| Q50219401 | Photo-induced iodination of aryl halides under very mild conditions. |
| Q80078279 | Photochemical determination of different DNA structures |
| Q33280940 | Photoconductive stimulation of neurons cultured on silicon wafers |
| Q45964578 | Photocrosslinking of glycoconjugates using metabolically incorporated diazirine-containing sugars. |
| Q33434373 | Phylogenetic classification and identification of bacteria by mass spectrometry |
| Q51032492 | Physiological 3D tissue model of the airway wall and mucosa. |
| Q48796064 | Piezo-actuated mouse intracytoplasmic sperm injection (ICSI). |
| Q90724236 | Pig lung transplant survival model |
| Q91283780 | Pipelines for cross-species and genome-wide prediction of long noncoding RNA binding |
| Q47705371 | Plasmonic ELISA for the detection of analytes at ultralow concentrations with the naked eye. |
| Q46098219 | Polyethylene glycol-based bidentate ligands to enhance quantum dot and gold nanoparticle stability in biological media |
| Q46027323 | Polymer-assisted deposition of homogeneous metal oxide films to produce nuclear targets. |
| Q125341639 | Pooled genetic perturbation screens with image-based phenotypes |
| Q41530610 | Population-specific genotype imputations using minimac or IMPUTE2. |
| Q88065767 | Porous nucleating agents for protein crystallization |
| Q52694903 | Positional cloning by fast-track SNP-mapping in Drosophila melanogaster. |
| Q58023457 | Positional proteomics: preparation of amino-terminal peptides as a strategy for proteome simplification and characterization |
| Q88037095 | Post-translational mutagenesis for installation of natural and unnatural amino acid side chains into recombinant proteins |
| Q52682286 | Practical Proline-catalyzed asymmetric Mannich reaction of aldehydes with N-Boc-imines. |
| Q51021820 | Practical and convenient synthesis of coumarins from phenols and propiolic acid esters. |
| Q43172288 | Practical cell-free protein synthesis system using purified wheat embryos |
| Q57369677 | Practical guidelines for dual-color fluorescence cross-correlation spectroscopy |
| Q34379924 | Pre-embedding immunogold labeling to optimize protein localization at subcellular compartments and membrane microdomains of leukocytes |
| Q38720592 | Precise and efficient scarless genome editing in stem cells using CORRECT. |
| Q41500111 | Precise manipulation of bacterial chromosomes by conjugative assembly genome engineering |
| Q123751123 | Precise motor mapping with transcranial magnetic stimulation |
| Q123558318 | Precise surface functionalization of PLGA particles for human T cell modulation |
| Q36886493 | Precision-engineering the Pseudomonas aeruginosa genome with two-step allelic exchange |
| Q34008437 | Predicting protein-protein interactions on a proteome scale by matching evolutionary and structural similarities at interfaces using PRISM. |
| Q43042116 | Predicting the age of mosquitoes using transcriptional profiles |
| Q29547194 | Predicting the effects of coding non-synonymous variants on protein function using the SIFT algorithm |
| Q24646028 | Preferential delivery of the Sleeping Beauty transposon system to livers of mice by hydrodynamic injection |
| Q37052877 | Preparation and biomedical applications of programmable and multifunctional DNA nanoflowers. |
| Q51061738 | Preparation and characterization of SNARE-containing nanodiscs and direct study of cargo release through fusion pores. |
| Q48169675 | Preparation and characterization of toxic Abeta aggregates for structural and functional studies in Alzheimer's disease research |
| Q37425491 | Preparation and functionalization of graphene nanocomposites for biomedical applications |
| Q53641650 | Preparation and guest-uptake protocol for a porous complex useful for 'crystal-free' crystallography. |
| Q48050521 | Preparation and implementation of optofluidic neural probes for in vivo wireless pharmacology and optogenetics |
| Q42931686 | Preparation and incubation of precision-cut liver and intestinal slices for application in drug metabolism and toxicity studies. |
| Q42510599 | Preparation and maintenance of single-cell micro-island cultures of basal forebrain neurons |
| Q33966046 | Preparation and purification of zinc sulfinate reagents for drug discovery |
| Q80093342 | Preparation and square wave electroporation of retinal explant cultures |
| Q40150097 | Preparation and use of Coppersensor-1, a synthetic fluorophore for live-cell copper imaging |
| Q46615555 | Preparation and use of Leadfluor-1, a synthetic fluorophore for live-cell lead imaging |
| Q33895555 | Preparation and use of MitoPY1 for imaging hydrogen peroxide in mitochondria of live cells |
| Q56990011 | Preparation and use of metal surface-immobilized DNA hairpins for the detection of oligonucleotides |
| Q46961556 | Preparation of 18F-labeled peptides using the copper(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition |
| Q80092946 | Preparation of N-succinimidyl 3-[*I]iodobenzoate: an agent for the indirect radioiodination of proteins |
| Q88414886 | Preparation of a Nile Red-Pd-based fluorescent CO probe and its imaging applications in vitro and in vivo |
| Q40136454 | Preparation of a Trp-BODIPY fluorogenic amino acid to label peptides for enhanced live-cell fluorescence imaging |
| Q80093474 | Preparation of a copper-based fluorescent probe for nitric oxide and its use in mammalian cultured cells |
| Q86539063 | Preparation of a cyanine-based fluorescent probe for highly selective detection of glutathione and its use in living cells and tissues of mice |
| Q35581405 | Preparation of abiotic polymer nanoparticles for sequestration and neutralization of a target peptide toxin. |
| Q38303100 | Preparation of aptamer-linked gold nanoparticle purple aggregates for colorimetric sensing of analytes |
| Q46896520 | Preparation of arylzinc reagents and their use in the rhodium-catalyzed asymmetric 1,4-addition for the synthesis of 2-aryl-4-piperidones |
| Q91322438 | Preparation of asymmetric phospholipid vesicles for use as cell membrane models |
| Q45986778 | Preparation of biogenic gas vesicle nanostructures for use as contrast agents for ultrasound and MRI. |
| Q48012836 | Preparation of biomimetic hierarchically helical fiber actuators from carbon nanotubes |
| Q52974425 | Preparation of biomimetic photoresponsive polymer springs. |
| Q39989123 | Preparation of carbon nanotube bioconjugates for biomedical applications |
| Q45720506 | Preparation of carboxylate derivatives of terpyridine via the furan pathway |
| Q39719756 | Preparation of cells for assessing ultrastructural localization of nanoparticles with transmission electron microscopy. |
| Q93023786 | Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNA |
| Q46896531 | Preparation of chiral derivatives of beta-Ala containing the alpha-phenylethyl group: useful starting materials for the asymmetric synthesis of beta-amino acids |
| Q48218987 | Preparation of chiral quantum dots |
| Q52370988 | Preparation of chiral-at-metal catalysts and their use in asymmetric photoredox chemistry. |
| Q48669738 | Preparation of compact biocompatible quantum dots using multicoordinating molecular-scale ligands based on a zwitterionic hydrophilic motif and lipoic acid anchors. |
| Q48301200 | Preparation of core-shell NaGdF4 nanoparticles doped with luminescent lanthanide ions to be used as upconversion-based probes. |
| Q53558299 | Preparation of cultured myofibers from larval salamander limbs for cellular plasticity studies. |
| Q84483057 | Preparation of cyclodextrin chiral stationary phases by organic soluble catalytic 'click' chemistry |
| Q84942978 | Preparation of dicationic palladium catalysts for asymmetric catalytic reactions |
| Q38296368 | Preparation of electrode-immobilized, redox-modified oligonucleotides for electrochemical DNA and aptamer-based sensing |
| Q47601082 | Preparation of glycoconjugates from unprotected carbohydrates for protein-binding studies |
| Q79845485 | Preparation of intact yeast artificial chromosome DNA for transgenesis of mice |
| Q46003839 | Preparation of integrin alpha(v)beta3-targeting Ab 38C2 constructs. |
| Q50882335 | Preparation of iron oxide-based calcium sensors for MRI. |
| Q81246984 | Preparation of large RNA oligonucleotides with complementary isotope-labeled segments for NMR structural studies |
| Q33310283 | Preparation of macromolecular complexes for cryo-electron microscopy |
| Q46715839 | Preparation of magnetic resonance contrast agents activated by beta-galactosidase |
| Q52734316 | Preparation of megabase-sized DNA from a variety of organisms using the nuclei method for advanced genomics research. |
| Q30365684 | Preparation of microcrystals in lipidic cubic phase for serial femtosecond crystallography. |
| Q48311587 | Preparation of molecularly imprinted polymers specific to glycoproteins, glycans and monosaccharides via boronate affinity controllable-oriented surface imprinting. |
| Q61632604 | Preparation of multifunctional nanoparticles and their assemblies |
| Q44313131 | Preparation of organotypic hippocampal slice cultures for long-term live imaging |
| Q48218802 | Preparation of organotypic hippocampal slice cultures: interface method |
| Q91595649 | Preparation of paper-based devices for reagentless electrochemical (bio)sensor strips |
| Q40025490 | Preparation of peptide-conjugated quantum dots for tumor vasculature-targeted imaging |
| Q46058273 | Preparation of phenols by phthaloyl peroxide-mediated oxidation of arenes |
| Q84862150 | Preparation of plant cells for transmission electron microscopy to optimize immunogold labeling of carbohydrate and protein epitopes |
| Q57798905 | Preparation of plant tissue to enable Spatial Transcriptomics profiling using barcoded microarrays |
| Q35707975 | Preparation of plants for developmental and cellular imaging by light-sheet microscopy |
| Q37376718 | Preparation of plasmonic vesicles from amphiphilic gold nanocrystals grafted with polymer brushes. |
| Q80093337 | Preparation of primary cultured mesenteric artery smooth muscle cells for fluorescent imaging and physiological studies |
| Q51571874 | Preparation of pyrenyl-based multifunctional nanocomposites for biomedical applications. |
| Q51032356 | Preparation of ready-to-use, storable and reconstituted type I collagen from rat tail tendon for tissue engineering applications. |
| Q33848902 | Preparation of reduced representation bisulfite sequencing libraries for genome-scale DNA methylation profiling |
| Q42872333 | Preparation of retinal explant cultures to study ex vivo tip endothelial cell responses |
| Q99616930 | Preparation of robust fluorescent probes for tracking endogenous formaldehyde in living cells and mouse tissue slices |
| Q46941932 | Preparation of site-specifically labeled fluorinated proteins for 19F-NMR structural characterization |
| Q63887479 | Preparation of substituted anilines from nitro compounds by using supported gold catalysts |
| Q36112722 | Preparation of supramolecular hydrogel-enzyme hybrids exhibiting biomolecule-responsive gel degradation. |
| Q43142486 | Preparation of symmetric and asymmetric aromatic azo compounds from aromatic amines or nitro compounds using supported gold catalysts |
| Q34063866 | Preparation of the caspase-3/7 substrate Ac-DEVD-pNA by solution-phase peptide synthesis |
| Q46944574 | Preparation of the functionalizable methionine surrogate azidohomoalanine via copper-catalyzed diazo transfer |
| Q24617371 | Preparation of the membrane-permeant biarsenicals FlAsH-EDT2 and ReAsH-EDT2 for fluorescent labeling of tetracysteine-tagged proteins |
| Q33898380 | Preparation of the planarian Schmidtea mediterranea for high-resolution histology and transmission electron microscopy |
| Q49491883 | Preparation of viable adult ventricular myocardial slices from large and small mammals. |
| Q48284677 | Preparation of visible-light-activated metal complexes and their use in photoredox/nickel dual catalysis. |
| Q38460051 | Preparation, application and testing of permanent antibacterial and antiviral coatings |
| Q38720595 | Preparation, characterization, and in vitro dosimetry of dispersed, engineered nanomaterials |
| Q90168554 | Preparation, functionalization and characterization of engineered carbon nanodots |
| Q46715819 | Preparative scale Baeyer-Villiger biooxidation at high concentration using recombinant Escherichia coli and in situ substrate feeding and product removal process |
| Q87843212 | Preparative scale and convenient synthesis of a water-soluble, deep cavitand |
| Q54431061 | Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems. |
| Q30846824 | Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments. |
| Q94671871 | Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography |
| Q31148892 | Preprocessing, classification modeling and feature selection using flow injection electrospray mass spectrometry metabolite fingerprint data |
| Q39287947 | Primary culture and immortalization of human fallopian tube secretory epithelial cells |
| Q47391234 | Primary culture and phenotyping of murine chondrocytes. |
| Q44237988 | Primary culture of Caenorhabditis elegans developing embryo cells for electrophysiological, cell biological and molecular studies |
| Q40124485 | Primary culture of bovine chromaffin cells |
| Q39465676 | Primary culture of chick, mouse or human neural crest cells |
| Q38433226 | Primary culture of ovarian surface epithelial cells and ascites-derived ovarian cancer cells from patients |
| Q58028272 | Primary support cultures of hippocampal and substantia nigra neurons |
| Q114109144 | Principles of reproducible metabolite profiling of enriched lymphocytes in tumors and ascites from human ovarian cancer |
| Q48076464 | Probe selection for DNA microarrays using OligoWiz. |
| Q80286038 | Probing 2-dimensional protein-protein interactions on model membranes |
| Q36406531 | Probing exchange kinetics and atomic resolution dynamics in high-molecular-weight complexes using dark-state exchange saturation transfer NMR spectroscopy. |
| Q83977304 | Probing localized neural mechanotransduction through surface-modified elastomeric matrices and electrophysiology |
| Q56974325 | Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry |
| Q52885230 | Procedures for the reconstruction, primary culture and experimental use of rainbow trout gill epithelia. |
| Q46941953 | Processing tissue and cells for transmission electron microscopy in diagnostic pathology and research |
| Q52603776 | Producing genome structure populations with the dynamic and automated PGS software. |
| Q41674068 | Production and analysis of immunomodulatory excretory-secretory products from the mouse gastrointestinal nematode Heligmosomoides polygyrus bakeri |
| Q36779416 | Production and characterization of adeno-associated viral vectors |
| Q46172190 | Production and purification of lentiviral vectors |
| Q46706919 | Production and quantification of sesquiterpenes in Saccharomyces cerevisiae, including extraction, detection and quantification of terpene products and key related metabolites. |
| Q53521087 | Production of a site- and phosphorylation state-specific antibody. |
| Q46767342 | Production of an acetone-butanol-ethanol mixture from Clostridium acetobutylicum and its conversion to high-value biofuels |
| Q84227282 | Production of avian retroviruses and tissue-specific somatic retroviral gene transfer in vivo using the RCAS/TVA system |
| Q30494830 | Production of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos |
| Q40227402 | Production of cloned mice by somatic cell nuclear transfer |
| Q82244988 | Production of compartmented cultures of rat sympathetic neurons |
| Q60681167 | Production of dissociated sensory neuron cultures and considerations for their use in studying neuronal function and plasticity |
| Q39732172 | Production of glycoprotein-deleted rabies viruses for monosynaptic tracing and high-level gene expression in neurons |
| Q36904417 | Production of hepatocyte-like cells from human pluripotent stem cells. |
| Q39068178 | Production of highly potent recombinant siRNAs in Escherichia coli |
| Q47299621 | Production of knock-in mice in a single generation from embryonic stem cells |
| Q39709056 | Production of mice using iPS cells and tetraploid complementation |
| Q81415175 | Production of neurons, astrocytes and oligodendrocytes from mammalian CNS stem cells |
| Q49985549 | Production of ready-to-use few-layer graphene in aqueous suspensions. |
| Q51032452 | Production of recombinant protein in Pichia pastoris by fermentation. |
| Q40063164 | Production of tunable nanomaterials using hierarchically assembled bacteriophages |
| Q38610131 | Production of unnaturally linked chimeric proteins using a combination of sortase-catalyzed transpeptidation and click chemistry |
| Q39870613 | Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors |
| Q34470014 | Profiling DNA methylome landscapes of mammalian cells with single-cell reduced-representation bisulfite sequencing |
| Q51986573 | Profiling of DNA replication timing in unsynchronized cell populations. |
| Q80198684 | Profiling of microRNA expression by mRAP |
| Q50690867 | Profluorescent protein fragments for fast bimolecular fluorescence complementation in vitro. |
| Q46754377 | Programmable reactivity-based one-pot oligosaccharide synthesis |
| Q89486200 | Programming DNA origami assembly for shape-resolved nanomechanical imaging labels |
| Q48219588 | Promoting the accumulation of tumor-specific T cells in tumor tissues by dendritic cell vaccines and chemokine-modulating agents. |
| Q34633979 | Pronuclear injection for the production of transgenic mice |
| Q50507537 | Prospective isolation of adult neural stem cells from the mouse subependymal zone. |
| Q48221591 | Protein chemical synthesis by α-ketoacid-hydroxylamine ligation. |
| Q101476554 | Protein higher-order-structure determination by fast photochemical oxidation of proteins and mass spectrometry analysis |
| Q53138602 | Protein labeling with the labeling precursor [(18)F]SiFA-SH for positron emission tomography. |
| Q33725480 | Protein misfolding cyclic amplification of infectious prions |
| Q33280961 | Protein profiling by the combination of two independent mass spectrometry techniques. |
| Q48238520 | Protein reconstitution into freestanding planar lipid membranes for electrophysiological characterization |
| Q30374281 | Protein structure homology modeling using SWISS-MODEL workspace. |
| Q27860664 | Protein structure prediction on the Web: a case study using the Phyre server |
| Q59385234 | Protein-based stable isotope probing |
| Q51642295 | Protein-observed (19)F-NMR for fragment screening, affinity quantification and druggability assessment. |
| Q36294652 | Proteome-wide enrichment of proteins modified by lysine methylation. |
| Q40623371 | Proteome-wide identification of SUMO modification sites by mass spectrometry |
| Q49985530 | Proteome-wide identification of ubiquitin interactions using UbIA-MS. |
| Q30460049 | Proteomic analysis of formalin-fixed paraffin-embedded tissue by MALDI imaging mass spectrometry |
| Q46048841 | Proteomics-based identification of low-abundance signaling and regulatory protein complexes in native plant tissues |
| Q91908223 | Protocol Update for large-scale genome and gene function analysis with the PANTHER classification system (v.14.0) |
| Q29614831 | Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips |
| Q53855190 | Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. |
| Q48379027 | Protocol for quantifying sound-sensing ability of Drosophila melanogaster. |
| Q82433535 | Protocol for solid-phase microextraction method development |
| Q34614573 | Protocol for the MPTP mouse model of Parkinson's disease |
| Q80285840 | Protocol for the analysis of n-alkanes and other plant-wax compounds and for their use as markers for quantifying the nutrient supply of large mammalian herbivores |
| Q82433547 | Protocol for the development of automated high-throughput SPME-GC methods for the analysis of volatile and semivolatile constituents in wine samples |
| Q50953427 | Protocol for the fabrication of enzymatically crosslinked gelatin microchannels for microfluidic cell culture. |
| Q34615353 | Protocol for the fast chromatin immunoprecipitation (ChIP) method |
| Q53486924 | Protocol for the induction of arthritis in C57BL/6 mice. |
| Q48765037 | Protocol for the production of viable bimaternal mouse embryos. |
| Q80188287 | Protocol for the purification of proteins from biological extracts for identification by mass spectrometry |
| Q83265692 | Protocol for the quantitative assessment of DNA concentration and damage (fragmentation and nicks) |
| Q33522558 | Protocol for the selection of single-domain antibody fragments by third generation intracellular antibody capture |
| Q57002741 | Protocols for growth and development of Dictyostelium discoideum |
| Q33305816 | Protocols for the in situ PCR-amplification and detection of mRNA and DNA sequences |
| Q43221614 | Protocols to detect senescence-associated beta-galactosidase (SA-betagal) activity, a biomarker of senescent cells in culture and in vivo |
| Q101166881 | Proximity labeling in mammalian cells with TurboID and split-TurboID |
| Q90036565 | Publisher Correction: Acute and rapid degradation of endogenous proteins by Trim-Away |
| Q91272526 | Publisher Correction: Application and optimization of CRISPR-Cas9-mediated genome engineering in axolotl (Ambystoma mexicanum) |
| Q57285529 | Publisher Correction: Assessing spatial pattern separation in rodents using the object pattern separation task |
| Q95596597 | Publisher Correction: Evoking and tracking zebrafish eye movement in multiple larvae with ZebEyeTrack |
| Q91637223 | Publisher Correction: Live imaging of stem cells in the germarium of the Drosophila ovary using a reusable gas-permeable imaging chamber |
| Q57793598 | Publisher Correction: PEG-4MAL hydrogels for human organoid generation, culture, and in vivo delivery |
| Q57804875 | Publisher Correction: Patch clamp recording from enteric neurons in situ |
| Q58561226 | Publisher Correction: PhIP-Seq characterization of serum antibodies using oligonucleotide-encoded peptidomes |
| Q91810345 | Publisher Correction: Sequencing cell-type-specific transcriptomes with SLAM-ITseq |
| Q90826029 | Publisher Correction: Surface-wetting characterization using contact-angle measurements |
| Q91937768 | Publisher Correction: Systemic AAV vectors for widespread and targeted gene delivery in rodents |
| Q103836842 | Publisher Correction: Tutorial: avoiding and correcting sample-induced spherical aberration artifacts in 3D fluorescence microscopy |
| Q54343517 | Pull-down of 5-hydroxymethylcytosine DNA using JBP1-coated magnetic beads. |
| Q48406868 | Pulsed-field gel electrophoresis. |
| Q46002482 | Purification and characterization of transcribed RNAs using gel filtration chromatography. |
| Q42523576 | Purification and culture of nerve growth factor receptor (p75)-expressing basal forebrain cholinergic neurons |
| Q34974257 | Purification and direct transformation of epithelial progenitor cells from primary human prostate |
| Q50953434 | Purification of antibodies using the synthetic affinity ligand absorbent MAbsorbent A2P. |
| Q46715823 | Purification of human plasma fibronectin using immobilized gelatin and Arg affinity chromatography |
| Q50629332 | Purification of matrix metalloproteinases by column chromatography. |
| Q31108033 | Purification of mitochondrial and plastid DNA. |
| Q80078317 | Purification of naturally occurring peptides by reversed-phase HPLC |
| Q47174725 | Purification of recombinant high molecular weight two-partner secretion proteins from Escherichia coli |
| Q52578111 | Purification of recombinant membrane proteins tagged with calmodulin-binding domains by affinity chromatography on calmodulin-agarose: example of nicotinamide nucleotide transhydrogenase. |
| Q93163072 | Purification of tubulin with controlled post-translational modifications by polymerization-depolymerization cycles |
| Q80643394 | Pyrosequencing protocol using a universal biotinylated primer for mutation detection and SNP genotyping |
| Q80643419 | Pyruvate aldolases in chiral carbon-carbon bond formation |
| Q54377523 | Quadratic isothermal amplification for the detection of microRNA. |
| Q90168559 | Qualifying antibodies for image-based immune profiling and multiplexed tissue imaging |
| Q56996522 | Qualitative PCR-ELISA protocol for the detection and typing of viral genomes |
| Q30539849 | Qualitative and quantitative metabolomic investigation of single neurons by capillary electrophoresis electrospray ionization mass spectrometry |
| Q62577374 | Quality assurance for polychromatic flow cytometry |
| Q34471362 | Quality assurance for polychromatic flow cytometry using a suite of calibration beads |
| Q28656219 | Quality control and conduct of genome-wide association meta-analyses |
| Q33706263 | Quantification of DNA damage products resulting from deamination, oxidation and reaction with products of lipid peroxidation by liquid chromatography isotope dilution tandem mass spectrometry |
| Q51580788 | Quantification of DNA methylation in electrofluidics chips (Bio-COBRA). |
| Q59173846 | Quantification of F2-isoprostanes as a biomarker of oxidative stress |
| Q99728288 | Quantification of SARS-CoV-2 neutralizing antibody by a pseudotyped virus-based assay |
| Q84489156 | Quantification of adenosine-to-inosine editing of microRNAs using a conventional method |
| Q82244059 | Quantification of beta-human papillomavirus DNA by real-time PCR |
| Q38776380 | Quantification of cancer cell extravasation in vivo. |
| Q48218772 | Quantification of cerebral amyloid angiopathy and parenchymal amyloid plaques with Congo red histochemical stain |
| Q30354112 | Quantification of free cysteines in membrane and soluble proteins using a fluorescent dye and thermal unfolding. |
| Q30422016 | Quantification of larval zebrafish motor function in multiwell plates using open-source MATLAB applications. |
| Q89792535 | Quantification of mRNA translation in live cells using single-molecule imaging |
| Q28296375 | Quantification of mRNA using real-time RT-PCR |
| Q38852230 | Quantification of nascent transcription by bromouridine immunocapture nuclear run-on RT-qPCR. |
| Q30542079 | Quantification of oxygen-induced retinopathy in the mouse: a model of vessel loss, vessel regrowth and pathological angiogenesis |
| Q58036615 | Quantification of starch in plant tissues |
| Q45807486 | Quantifying DNA double-strand breaks induced by site-specific endonucleases in living cells by ligation-mediated purification. |
| Q57825385 | Quantifying cellular adhesion to extracellular matrix components by single-cell force spectroscopy |
| Q33495521 | Quantifying cellular interaction dynamics in 3D fluorescence microscopy data |
| Q39199394 | Quantifying macromolecular interactions in living cells using FRET two-hybrid assays |
| Q34895651 | Quantifying protein-protein interactions in high throughput using protein domain microarrays |
| Q52576387 | Quantifying solid stress and elastic energy from excised or in situ tumors. |
| Q53089446 | Quantifying spatial correlations of fluorescent markers using enhanced background reduction with protein proximity index and correlation coefficient estimations. |
| Q37586266 | Quantifying spatial organization in point-localization superresolution images using pair correlation analysis |
| Q87658351 | Quantifying the forces guiding microbial cell adhesion using single-cell force spectroscopy |
| Q30537588 | Quantifying the transcriptional output of single alleles in single living mammalian cells |
| Q38689372 | Quantifying transcription factor-DNA binding in single cells in vivo with photoactivatable fluorescence correlation spectroscopy. |
| Q82715952 | Quantitation of human mammary epithelial stem cells with in vivo regenerative properties using a subrenal capsule xenotransplantation assay |
| Q38838633 | Quantitative 3D structured illumination microscopy of nuclear structures. |
| Q51887279 | Quantitative DNase footprint titration: a tool for analyzing the energetics of protein-DNA interactions. |
| Q33455428 | Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples |
| Q34581232 | Quantitative analysis of chromosome conformation capture assays (3C-qPCR). |
| Q62070306 | Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography–mass spectrometry |
| Q90428279 | Quantitative analysis of newly synthesized proteins |
| Q28543179 | Quantitative analysis of ribonucleoside modifications in tRNA by HPLC-coupled mass spectrometry |
| Q35204646 | Quantitative analysis of triple-mutant genetic interactions |
| Q38974632 | Quantitative and unbiased analysis of directional persistence in cell migration |
| Q41890546 | Quantitative assays for esterified oxylipins generated by immune cells |
| Q36466966 | Quantitative assessment of RNA-protein interactions with high-throughput sequencing-RNA affinity profiling. |
| Q35516131 | Quantitative assessment of angiogenesis, perfused blood vessels and endothelial tip cells in the postnatal mouse brain. |
| Q47794539 | Quantitative bioluminescence imaging of tumor-targeting bacteria in living animals |
| Q90596410 | Quantitative cross-linking/mass spectrometry to elucidate structural changes in proteins and their complexes |
| Q104111451 | Quantitative determination of phosphatidylethanol in dried blood spots for monitoring alcohol abstinence |
| Q83977291 | Quantitative electron microscopy for the nanoscale analysis of membrane lipid distribution |
| Q80285983 | Quantitative gas chromatography mass spectrometric analysis of 2'-deoxyinosine in tissue DNA |
| Q30494075 | Quantitative imaging of collective cell migration during Drosophila gastrulation: multiphoton microscopy and computational analysis |
| Q82893043 | Quantitative imaging of membrane lipid order in cells and organisms |
| Q92887106 | Quantitative imaging of sleep behavior in Caenorhabditis elegans and larval Drosophila melanogaster |
| Q55519780 | Quantitative mapping of fluorescently tagged cellular proteins using FCS-calibrated four-dimensional imaging |
| Q36440706 | Quantitative measurement of transcriptional inhibition and mutagenesis induced by site-specifically incorporated DNA lesions in vitro and in vivo |
| Q30560325 | Quantitative monitoring of mouse lung tumors by magnetic resonance imaging |
| Q44427834 | Quantitative multiplex degenerate PCR for human endogenous retrovirus expression profiling |
| Q112700669 | Quantitative nucleotide resolution profiling of RNA cytidine acetylation by ac4C-seq |
| Q29615372 | Quantitative prediction of cellular metabolism with constraint-based models: the COBRA Toolbox |
| Q28246991 | Quantitative prediction of cellular metabolism with constraint-based models: the COBRA Toolbox v2.0 |
| Q53281395 | Quantitative prediction of charge mobilities of π-stacked systems by first-principles simulation. |
| Q48285696 | Quantitative profiling of the protein coronas that form around nanoparticles. |
| Q80092795 | Quantitative proteome analysis using isotope-coded affinity tags and mass spectrometry |
| Q80285868 | Quantitative proteomic approach to study subcellular localization of membrane proteins |
| Q28271745 | Quantitative proteomics using stable isotope labeling with amino acids in cell culture |
| Q38676266 | Quantitative proteomics: challenges and opportunities in basic and applied research |
| Q48021398 | Quantitative, multiplexed workflow for deep analysis of human blood plasma and biomarker discovery by mass spectrometry. |
| Q92883243 | Quantum diamond spectrometer for nanoscale NMR and ESR spectroscopy |
| Q46102167 | Quartz crystal microbalance with dissipation monitoring of supported lipid bilayers on various substrates |
| Q80078253 | Quick and easy yeast transformation using the LiAc/SS carrier DNA/PEG method |
| Q93163065 | R-ChIP for genome-wide mapping of R-loops by using catalytically inactive RNASEH1 |
| Q38497690 | RGB marking with lentiviral vectors for multicolor clonal cell tracking |
| Q34003299 | RIP-Chip: the isolation and identification of mRNAs, microRNAs and protein components of ribonucleoprotein complexes from cell extracts. |
| Q92512571 | RNA sequencing and swarm intelligence-enhanced classification algorithm development for blood-based disease diagnostics using spliced blood platelet RNA |
| Q51710184 | RNA stable-isotope probing. |
| Q33536986 | RNA-Seq analysis to capture the transcriptome landscape of a single cell |
| Q55554519 | RNAi screens in Caenorhabditis elegans in a 96-well liquid format and their application to the systematic identification of genetic interactions |
| Q41147236 | RNAi-based biosynthetic pathway screens to identify in vivo functions of non-nucleic acid-based metabolites such as lipids |
| Q92955683 | RNase A treatment and reconstitution with DNA damage response RNA in living cells as a tool to study the role of non-coding RNA in the formation of DNA damage response foci |
| Q92154263 | RNase H-dependent PCR-enabled T-cell receptor sequencing for highly specific and efficient targeted sequencing of T-cell receptor mRNA for single-cell and repertoire analysis |
| Q30314932 | Radiolabeling of DOTA-like conjugated peptides with generator-produced (68)Ga and using NaCl-based cationic elution method. |
| Q46193111 | Radiolabeling of HYNIC-annexin V with technetium-99m for in vivo imaging of apoptosis |
| Q80093411 | Radiolabeling of TETA- and CB-TE2A-conjugated peptides with copper-64 |
| Q53150856 | Radiometric and spectrophotometric in vitro assays of glycosyltransferases involved in plant cell wall carbohydrate biosynthesis. |
| Q102369571 | Radiosynthesis of [18F]SiFAlin-TATE for clinical neuroendocrine tumor positron emission tomography |
| Q46024080 | Raft-like membranes from the trans-Golgi network and endosomal compartments. |
| Q34614032 | Raman and SERS microscopy for molecular imaging of live cells |
| Q100416124 | Rapid (30-second), equipment-free purification of nucleic acids using easy-to-make dipsticks |
| Q84261518 | Rapid DNA detection by beacon-assisted detection amplification |
| Q46521680 | Rapid and simple isolation of vascular, epidermal and mesophyll cells from plant leaf tissue. |
| Q33706964 | Rapid bacterial artificial chromosome modification for large-scale mouse transgenesis |
| Q46484483 | Rapid characterization of complex viscous samples at molecular levels by neutral desorption extractive electrospray ionization mass spectrometry |
| Q47687623 | Rapid curation of gene disruption collections using Knockout Sudoku. |
| Q35238418 | Rapid editing and evolution of bacterial genomes using libraries of synthetic DNA. |
| Q40150139 | Rapid functional assays of intracellular Ca2+ channels. |
| Q34953172 | Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen |
| Q46497192 | Rapid generation of knockdown transgenic mice by silencing lentiviral vectors |
| Q46465136 | Rapid identification of causal mutations in tomato EMS populations via mapping-by-sequencing. |
| Q89723601 | Rapid identification of human mast cell degranulation regulators using functional genomics coupled to high-resolution confocal microscopy |
| Q80286000 | Rapid identification of unknown heteroplasmic mutations across the entire human mitochondrial genome with mismatch-specific Surveyor Nuclease |
| Q48256635 | Rapid immunoprecipitation mass spectrometry of endogenous proteins (RIME) for analysis of chromatin complexes. |
| Q88037099 | Rapid immunopurification of mitochondria for metabolite profiling and absolute quantification of matrix metabolites |
| Q43030518 | Rapid in situ codetection of noncoding RNAs and proteins in cells and formalin-fixed paraffin-embedded tissue sections without protease treatment. |
| Q112331100 | Rapid in situ identification of biological specimens via DNA amplicon sequencing using miniaturized laboratory equipment |
| Q51549279 | Rapid isolation of antigen-specific antibody-secreting cells using a chip-based immunospot array. |
| Q35148885 | Rapid isolation of single malaria parasite-infected red blood cells by cell sorting |
| Q80188296 | Rapid measurement of total antioxidant capacity in plants |
| Q89475588 | Rapid online buffer exchange for screening of proteins, protein complexes and cell lysates by native mass spectrometry |
| Q112720003 | Rapid ordering of barcoded transposon insertion libraries of anaerobic bacteria |
| Q80093460 | Rapid preparation of the mitotic kinesin Eg5 inhibitor monastrol using controlled microwave-assisted synthesis |
| Q50489884 | Rapid separation of Arabidopsis male gametophyte developmental stages using a Percoll gradient. |
| Q81247000 | Rapid solid-phase synthesis of a calmodulin-binding peptide using controlled microwave irradiation |
| Q48911126 | Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants. |
| Q33732610 | Raster image correlation spectroscopy in live cells |
| Q40150124 | Rational design and in vitro and in vivo delivery of Dicer substrate siRNA. |
| Q29028421 | Rayburst sampling, an algorithm for automated three-dimensional shape analysis from laser scanning microscopy images |
| Q91560301 | Reading and writing digital data in DNA |
| Q33283987 | Real-time assessment of three-dimensional cell aggregation in rotating wall vessel bioreactors in vitro |
| Q36513925 | Real-time detection of acetylcholine release from the human endocrine pancreas |
| Q33925519 | Real-time fluorescence assays to monitor duplex unwinding and ATPase activities of helicases |
| Q91974086 | Real-time imaging of multivesicular body-plasma membrane fusion to quantify exosome release from single cells |
| Q47829228 | Real-time in vivo imaging of transgenic bioluminescent blood stages of rodent malaria parasites in mice. |
| Q64448222 | Real-time microscopy and physical perturbation of bacterial pili using maleimide-conjugated molecules |
| Q92564940 | Real-time physiological measurements of oxygen using a non-invasive self-referencing optical fiber microsensor |
| Q35000412 | Real-time single-molecule coimmunoprecipitation of weak protein-protein interactions |
| Q90288980 | Real-time visualization of mutations and their fitness effects in single bacteria |
| Q40648473 | RecET direct cloning and Redαβ recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression |
| Q50690790 | Recombinant protein purification by self-cleaving aggregation tag. |
| Q28307909 | Recombineering: a homologous recombination-based method of genetic engineering |
| Q57975247 | Reconstituting actomyosin-dependent mechanosensitive protein complexes in vitro |
| Q96766167 | Reconstitution and real-time quantification of membrane remodeling by single proteins and protein complexes |
| Q47810159 | Reconstitution of mouse oogenesis in a dish from pluripotent stem cells |
| Q35764449 | Reconstitution of the cell cycle-regulated Golgi disassembly and reassembly in a cell-free system |
| Q80092812 | Reconstruction of human mammary tissues in a mouse model |
| Q50433436 | Recording of mechanosensitive currents using piezoelectrically driven mechanostimulator. |
| Q92564929 | Recording transcriptional histories using Record-seq |
| Q33331330 | Reduction of dynamic protein concentration range of biological extracts for the discovery of low-abundance proteins by means of hexapeptide ligand library |
| Q90591822 | Reductive amination using cobalt-based nanoparticles for synthesis of amines |
| Q99728283 | Reference-free deconvolution, visualization and interpretation of complex DNA methylation data using DecompPipeline, MeDeCom and FactorViz |
| Q48318611 | Regenerating cortical connections in a dish: the entorhino-hippocampal organotypic slice co-culture as tool for pharmacological screening of molecules promoting axon regeneration. |
| Q92955693 | Regeneration of a bioengineered 3D integumentary organ system from iPS cells |
| Q34407494 | Regioselective monodeprotection of peracetylated carbohydrates. |
| Q46809750 | Regioselective one-pot protection of glucose |
| Q45752298 | Relative and absolute determination of fluorescence quantum yields of transparent samples |
| Q44796302 | Relative quantification of proteasome activity by activity-based protein profiling and LC-MS/MS. |
| Q42806910 | Remodeling somatic nuclei via exogenous expression of protamine 1 to create spermatid-like structures for somatic nuclear transfer |
| Q35642243 | Removable cranial windows for long-term imaging in awake mice |
| Q80285885 | Repeated measures of shoaling tendency in zebrafish (Danio rerio) and other small teleost fishes |
| Q47716515 | Repeated positive fighting experience in male inbred mice |
| Q89242862 | Reply to 'Defining distance restraints in HADDOCK' |
| Q94671866 | Reproducible molecular networking of untargeted mass spectrometry data using GNPS |
| Q56557176 | Reproducible subcutaneous transplantation of cell sheets into recipient mice |
| Q89486219 | Reproducible workflow for multiplexed deep-scale proteome and phosphoproteome analysis of tumor tissues by liquid chromatography-mass spectrometry |
| Q46114696 | Reprogramming fibroblasts toward cardiomyocytes, neural stem cells and hepatocytes by cell activation and signaling-directed lineage conversion |
| Q39463637 | Reprogramming human fibroblasts to pluripotency using modified mRNA. |
| Q50949635 | Reprogramming of mouse fibroblasts into cardiomyocyte-like cells in vitro. |
| Q41146288 | Reprogramming of mouse retinal neurons and standardized quantification of their differentiation in 3D retinal cultures |
| Q33303180 | Reprogramming the amino-acid substrate specificity of orthogonal aminoacyl-tRNA synthetases to expand the genetic code of eukaryotic cells |
| Q33684459 | Resin-assisted enrichment of thiols as a general strategy for proteomic profiling of cysteine-based reversible modifications |
| Q90439831 | Resolution of the DNA methylation state of single CpG dyads using in silico strand annealing and WGBS data |
| Q33965294 | Resolving genomic disorder-associated breakpoints within segmental DNA duplications using massively parallel sequencing |
| Q31133761 | Resolving macromolecular structures from electron cryo-tomography data using subtomogram averaging in RELION |
| Q80093356 | Restriction landmark genomic scanning |
| Q87366003 | Retraction: Reproducible subcutaneous transplantation of cell sheets into recipient mice |
| Q50861897 | Retrovirally mediated telomerase immortalization of neural progenitor cells. |
| Q46347673 | Retrovirus-mediated single-cell gene knockout technique in adult newborn neurons in vivo |
| Q111149750 | Revealing nascent RNA processing dynamics with nano-COP |
| Q88910889 | Revealing the architecture of protein complexes by an orthogonal approach combining HDXMS, CXMS, and disulfide trapping |
| Q49057103 | Reverse MAPPIT detects disruptors of protein-protein interactions in human cells. |
| Q38303089 | Reverse engineering cellular networks. |
| Q53561729 | Reverse transfection on cell arrays for high content screening microscopy. |
| Q33381529 | Reverse-phase protein lysate microarrays for cell signaling analysis |
| Q46974311 | Reversible DNA encapsulation in silica to produce ROS-resistant and heat-resistant synthetic DNA 'fossils'. |
| Q57362379 | Rice blast infection of Brachypodium distachyon as a model system to study dynamic host/pathogen interactions |
| Q43178413 | Robotic multiwell planar patch-clamp for native and primary mammalian cells |
| Q33288504 | Robust analysis of 5'-transcript ends: a high-throughput protocol for characterization of sequence diversity of transcription start sites |
| Q34580603 | Robust phosphoproteome enrichment using monodisperse microsphere-based immobilized titanium (IV) ion affinity chromatography |
| Q40563355 | Routine in vitro culture of P. falciparum gametocytes to evaluate novel transmission-blocking interventions. |
| Q24644270 | SCWRL and MolIDE: computer programs for side-chain conformation prediction and homology modeling |
| Q90242502 | SHERLOCK: nucleic acid detection with CRISPR nucleases |
| Q48094713 | SIFT missense predictions for genomes |
| Q37242476 | SILEC: a protocol for generating and using isotopically labeled coenzyme A mass spectrometry standards |
| Q42545602 | SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs |
| Q80092956 | Sample extraction techniques for enhanced proteomic analysis of plant tissues |
| Q48112280 | Sample preparation for high-resolution 3D confocal imaging of mouse skeletal tissue |
| Q87746574 | Scalable and chromatography-free synthesis of 2-(2-formylalkyl)arenecarboxylic acid derivatives through the supramolecularly controlled hydroformylation of vinylarene-2-carboxylic acids |
| Q112723582 | Scalable characterization of the PAM requirements of CRISPR-Cas enzymes using HT-PAMDA |
| Q83993769 | Scalable expansion of human pluripotent stem cells in suspension culture |
| Q48279030 | Scalable synthesis and post-modification of a mesoporous metal-organic framework called NU-1000. |
| Q125341857 | Scaling up reproducible research for single-cell transcriptomics using MetaNeighbor |
| Q48230748 | Scototaxis as anxiety-like behavior in fish. |
| Q41993913 | Screening and large-scale expression of membrane proteins in mammalian cells for structural studies |
| Q33667656 | Screening individual hybridomas by microengraving to discover monoclonal antibodies |
| Q48925635 | Seamless genome editing in human pluripotent stem cells using custom endonuclease-based gene targeting and the piggyBac transposon. |
| Q34189006 | Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure |
| Q54389645 | Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro. |
| Q35094080 | Segmentation and quantification of subcellular structures in fluorescence microscopy images using Squassh. |
| Q52926243 | Selecting effective siRNAs based on guide RNA structure. |
| Q49052034 | Selecting protein N-terminal peptides by combined fractional diagonal chromatography. |
| Q33280955 | Selection and characterization of large collections of peptide aptamers through optimized yeast two-hybrid procedures |
| Q47829242 | Selection by flow-sorting of genetically transformed, GFP-expressing blood stages of the rodent malaria parasite, Plasmodium berghei. |
| Q33859354 | Selection of antibodies against a single rare cell present in a heterogeneous population using phage display. |
| Q33305820 | Selection of human antibody fragments by phage display |
| Q31025344 | Selection of proteins with desired properties from natural proteome libraries using mRNA display |
| Q29617336 | Selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE): quantitative RNA structure analysis at single nucleotide resolution |
| Q35795056 | Selective 2'-hydroxyl acylation analyzed by primer extension and mutational profiling (SHAPE-MaP) for direct, versatile and accurate RNA structure analysis |
| Q37082884 | Selective 2'-hydroxyl acylation analyzed by protection from exoribonuclease (RNase-detected SHAPE) for direct analysis of covalent adducts and of nucleotide flexibility in RNA |
| Q36856260 | Selective amplification and sequencing of cyclic phosphate-containing RNAs by the cP-RNA-seq method |
| Q36126099 | Selective chemoprecipitation to enrich nitropeptides from complex proteomes for mass-spectrometric analysis |
| Q42787023 | Selective enrichment of sialic acid-containing glycopeptides using titanium dioxide chromatography with analysis by HILIC and mass spectrometry |
| Q80829937 | Selective isolation of genomic loci from complex genomes by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae |
| Q89475567 | Selective isolation of large segments from individual microbial genomes and environmental DNA samples using transformation-associated recombination cloning in yeast |
| Q50014466 | Selective monitoring of ubiquitin signals with genetically encoded ubiquitin chain-specific sensors. |
| Q80422766 | Selective proliferation of rat hepatocyte progenitor cells in serum-free culture |
| Q35020044 | Selective ribosome profiling as a tool for studying the interaction of chaperones and targeting factors with nascent polypeptide chains and ribosomes |
| Q92093782 | Selective ribosome profiling to study interactions of translating ribosomes in yeast |
| Q84006700 | Selective synthesis of 3-hydroxy acids from Meldrum's acids using SmI2-H2O |
| Q80093511 | Selenium modification of nucleic acids: preparation of oligonucleotides with incorporated 2'-SeMe-uridine for crystallographic phasing of nucleic acid structures |
| Q53742113 | Selenium modification of nucleic acids: preparation of phosphoroselenoate derivatives for crystallographic phasing of nucleic acid structures. |
| Q92669029 | Self-assembly and characterization of 2D plasmene nanosheets |
| Q38741388 | Self-organization of human embryonic stem cells on micropatterns |
| Q41761743 | Semiautomated and rapid quantification of nucleic acid footprinting and structure mapping experiments |
| Q35169616 | Semiautomated correlative 3D electron microscopy of in vivo-imaged axons and dendrites |
| Q51539171 | Sensitive detection of chromatin coassociations using enhanced chromosome conformation capture on chip. |
| Q43268254 | Separation and detection of large phosphoproteins using Phos-tag SDS-PAGE. |
| Q36148292 | Separation and parallel sequencing of the genomes and transcriptomes of single cells using G&T-seq |
| Q83131531 | Separation of isomeric octadecenoic fatty acids in partially hydrogenated vegetable oils as p-methoxyphenacyl esters using a single-column silver ion high-performance liquid chromatography (Ag-HPLC) |
| Q45239845 | Separation of sedimentary micron-sized particles for palaeoceanography and calcareous nannoplankton biogeochemistry. |
| Q80093348 | Sequence-specific amplification polymorphisms (SSAPs): a multi-locus approach for analyzing transposon insertions |
| Q50623862 | Sequencing and cloning of antigen-specific antibodies from mouse memory B cells. |
| Q67199182 | Sequencing cell-type-specific transcriptomes with SLAM-ITseq |
| Q33280989 | Sequencing complete mitochondrial and plastid genomes |
| Q92164090 | Sequential phosphoproteomics and N-glycoproteomics of plasma-derived extracellular vesicles |
| Q33280962 | Serial analysis of binding elements for human transcription factors |
| Q33283977 | Serial analysis of gene expression |
| Q40227986 | Serial sectioning and electron microscopy of large tissue volumes for 3D analysis and reconstruction: a case study of the calyx of Held |
| Q36154852 | Serial sequencing of isolength RAD tags for cost-efficient genome-wide profiling of genetic and epigenetic variations |
| Q30578549 | Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging |
| Q53794325 | Shear-thinning and self-healing hydrogels as injectable therapeutics and for 3D-printing. |
| Q82767630 | Short and scalable synthesis of an anhydride precursor of the environment-sensitive fluorophore 6-dimethylaminonaphthalimide |
| Q80092871 | Silencing gene expression by targeting chromosomal DNA with antigene peptide nucleic acids and duplex RNAs |
| Q46850564 | Silencing of mammalian genes by tetracycline-inducible shRNA expression |
| Q53557576 | Silver staining DNA in polyacrylamide gels. |
| Q42384963 | Silver staining of proteins in polyacrylamide gels |
| Q28647954 | Similarity-based modeling in large-scale prediction of drug-drug interactions |
| Q36295144 | Simple multiplexed PCR-based barcoding of DNA for ultrasensitive mutation detection by next-generation sequencing. |
| Q46754395 | Simple protocols for NMR analysis of the enantiomeric purity of chiral diols |
| Q46754400 | Simple protocols for NMR analysis of the enantiomeric purity of chiral primary amines |
| Q33518424 | Simultaneous analysis of reactive oxygen species and reduced glutathione content in living cells by polychromatic flow cytometry |
| Q58423536 | Simultaneous analysis of relative protein expression levels across multiple samples using iTRAQ isobaric tags with 2D nano LC–MS/MS |
| Q36427009 | Simultaneous detection of apoptosis and mitochondrial superoxide production in live cells by flow cytometry and confocal microscopy |
| Q50695605 | Simultaneous imaging of morphological plasticity and calcium dynamics in dendrites. |
| Q40473386 | Simultaneous in vitro molecular screening of protein-peptide interactions by flow cytometry, using six Bcl-2 family proteins as examples. |
| Q34414411 | Simultaneous isolation of high-quality DNA, RNA, miRNA and proteins from tissues for genomic applications |
| Q37366057 | Simultaneous knockdown of the expression of two genes using multiple shRNAs and subsequent knock-in of their expression |
| Q47877296 | Simultaneous lipid and content mixing assays for in vitro reconstitution studies of synaptic vesicle fusion |
| Q41155600 | Simultaneous measurement of genome-wide transcription elongation speeds and rates of RNA polymerase II transition into active elongation with 4sUDRB-seq. |
| Q47675102 | Simultaneous measurement of sleep and feeding in individual Drosophila. |
| Q80078271 | Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis |
| Q92095849 | Simultaneous noninvasive recording of electrocardiogram and skin sympathetic nerve activity (neuECG) |
| Q38778123 | Simultaneous quantification of N- and O-glycans using a solid-phase method |
| Q94461916 | Simultaneous quantification of protein-DNA interactions and transcriptomes in single cells with scDam&T-seq |
| Q52431349 | Simultaneous reprogramming and gene editing of human fibroblasts. |
| Q112709971 | Simultaneous ribosome profiling of hundreds of microbes from the human microbiome |
| Q33906901 | Single cell-resolution western blotting. |
| Q63502605 | Single nucleotide polymorphism (SNP) allele frequency estimation in DNA pools using Pyrosequencing™ |
| Q46896525 | Single nucleotide polymorphism detection by polymerase chain reaction-restriction fragment length polymorphism. |
| Q35652405 | Single prokaryotic cell isolation and total transcript amplification protocol for transcriptomic analysis |
| Q54437706 | Single protein production (SPP) system in Escherichia coli. |
| Q50690831 | Single- and dual-parameter FRET kinase probes based on pleckstrin. |
| Q34045326 | Single-cell Hi-C for genome-wide detection of chromatin interactions that occur simultaneously in a single cell. |
| Q125432767 | Single-cell RNA-seq of maize meiocytes and pollen grains |
| Q30433182 | Single-cell analysis and sorting using droplet-based microfluidics |
| Q48320117 | Single-cell axotomy of cultured hippocampal neurons integrated in neuronal circuits. |
| Q36215738 | Single-cell barcoding and sequencing using droplet microfluidics |
| Q98623950 | Single-cell imaging of human cancer xenografts using adult immunodeficient zebrafish |
| Q46595050 | Single-cell in situ imaging of palmitoylation in fatty-acylated proteins |
| Q39642934 | Single-cell in vivo imaging of adult neural stem cells in the zebrafish telencephalon |
| Q33310279 | Single-cell isolation from cell suspensions and whole genome amplification from single cells to provide templates for CGH analysis |
| Q38694712 | Single-cell mechanogenetics using monovalent magnetoplasmonic nanoparticles. |
| Q47266397 | Single-cell microscopy of suspension cultures using a microfluidics-assisted cell screening platform |
| Q36368576 | Single-cell template strand sequencing by Strand-seq enables the characterization of individual homologs. |
| Q40050365 | Single-channel recording of ligand-gated ion channels |
| Q33957080 | Single-mRNA counting using fluorescent in situ hybridization in budding yeast |
| Q59785243 | Single-mRNA detection in living S. cerevisiae using a re-engineered MS2 system |
| Q48101927 | Single-molecule fluorescence imaging to quantify membrane protein dynamics and oligomerization in living plant cells. |
| Q47770514 | Single-molecule mRNA detection and counting in mammalian tissue. |
| Q30539801 | Single-molecule pull-down for studying protein interactions. |
| Q39480677 | Single-molecule recognition force spectroscopy of transmembrane transporters on living cells |
| Q45981879 | Single-nucleotide polymorphism (SNP) genotyping using cationic conjugated polymers in homogeneous solution. |
| Q33283978 | Single-nucleotide polymorphisms: analysis by mass spectrometry |
| Q93337908 | Single-pot, solid-phase-enhanced sample preparation for proteomics experiments |
| Q56958769 | Single-strand conformation polymorphism (SSCP) for the analysis of genetic variation |
| Q57807209 | Single-strand conformation polymorphism analysis using capillary array electrophoresis for large-scale mutation detection |
| Q34620490 | Single-stranded DNA library preparation for the sequencing of ancient or damaged DNA. |
| Q51542428 | Single-tube linear DNA amplification for genome-wide studies using a few thousand cells. |
| Q80093327 | Single-tube reaction using peptide nucleic acid as both PCR clamp and sensor probe for the detection of rare mutations |
| Q114109150 | Sister chromatid–sensitive Hi-C to map the conformation of replicated genomes |
| Q37417814 | Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins |
| Q48634054 | Site-directed spin-labeling of nucleotides and the use of in-cell EPR to determine long-range distances in a biologically relevant environment. |
| Q46850578 | Site-selective glycosylation of proteins: creating synthetic glycoproteins |
| Q41877585 | Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions |
| Q41812690 | Site-specific N-terminal labeling of proteins using sortase-mediated reactions |
| Q45738787 | Site-specific NMR mapping and time-resolved monitoring of serine and threonine phosphorylation in reconstituted kinase reactions and mammalian cell extracts. |
| Q44829143 | Site-specific chemical modification of antibody fragments using traceless cleavable linkers |
| Q36399831 | Site-specific chemical protein conjugation using genetically encoded aldehyde tags |
| Q42993873 | Site-specific fluorescent probing of RNA molecules by unnatural base-pair transcription for local structural conformation analysis |
| Q50989020 | Site-specific incorporation of fluorotyrosines into the R2 subunit of E. coli ribonucleotide reductase by expressed protein ligation. |
| Q57235857 | Site-specific incorporation of nitroxide spin-labels into 2′-positions of nucleic acids |
| Q57894888 | Site-specific incorporation of non-natural amino acids into proteins in mammalian cells with an expanded genetic code |
| Q80092828 | Site-specific protein labeling by Sfp phosphopantetheinyl transferase |
| Q28829219 | Site-specific protein labeling using PRIME and chelation-assisted click chemistry |
| Q38541930 | Site-specific protein modification using immobilized sortase in batch and continuous-flow systems |
| Q39814223 | Six alternative proteases for mass spectrometry-based proteomics beyond trypsin. |
| Q89603320 | Skilled reaching tasks for head-fixed mice using a robotic manipulandum |
| Q34035385 | Sleeping Beauty transposon mutagenesis in rat spermatogonial stem cells |
| Q84616316 | Slide preparation for single-cell-resolution imaging of fluorescent proteins in their three-dimensional near-native environment |
| Q38112629 | Small-molecule ligand docking into comparative models with Rosetta |
| Q91560309 | Small-scale approach for precrystallization screening in GPCR X-ray crystallography |
| Q44391903 | Small-scale isolation of synaptic vesicles from mammalian brain |
| Q57235341 | Small-seq for single-cell small-RNA sequencing |
| Q107215771 | Snapshot photoacoustic topography through an ergodic relay of optical absorption in vivo |
| Q28275021 | Soft lithography for micro- and nanoscale patterning |
| Q50473036 | Solid-phase asymmetric synthesis using a polymer-supported chiral Evans'-type oxazolidin-2-one. |
| Q34271181 | Solid-phase extraction of N-linked glycopeptides |
| Q51672419 | Solid-phase microextraction to determine micropollutant-macromolecule partition coefficients. |
| Q52921616 | Solid-phase peptide synthesis: from standard procedures to the synthesis of difficult sequences. |
| Q34748392 | Solid-phase proximity ligation assays for individual or parallel protein analyses with readout via real-time PCR or sequencing |
| Q87270884 | Solid-phase synthesis of molecularly imprinted nanoparticles |
| Q46664266 | Solid-phase synthesis of pseudo-complementary peptide nucleic acids |
| Q36074865 | Solid-phase synthesis of short α-helices stabilized by the hydrogen bond surrogate approach |
| Q36379292 | Solid-phase synthesis, cyclization, and site-specific functionalization of aziridine-containing tetrapeptides |
| Q99616931 | Solid-state 31P NMR mapping of active centers and relevant spatial correlations in solid acid catalysts |
| Q91974099 | Solid-state nanopore fabrication by automated controlled breakdown |
| Q91595670 | Solidifying framework nucleic acids with silica |
| Q92596504 | Solvent-assisted preparation of supported lipid bilayers |
| Q80092901 | Southern blotting |
| Q38294566 | Southwestern blotting in investigating transcriptional regulation |
| Q36282263 | Spatial transcriptomic analysis of cryosectioned tissue samples with Geo-seq |
| Q46715828 | Spatially and temporally controlled electroporation of early chick embryos |
| Q98184353 | Spatially resolved analysis of FFPE tissue proteomes by quantitative mass spectrometry |
| Q36891427 | Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2. |
| Q36634489 | Spectral karyotyping analysis of human and mouse chromosomes |
| Q88721981 | Spectrofluorimetric quantification of antibiotic drug concentration in bacterial cells for the characterization of translocation across bacterial membranes |
| Q93184588 | Speed breeding in growth chambers and glasshouses for crop breeding and model plant research |
| Q30052394 | Spheroid-based drug screen: considerations and practical approach |
| Q51929209 | Spheroid-based human endothelial cell microvessel formation in vivo. |
| Q59457249 | Spin labeling of oligonucleotides with the nitroxide TPA and use of PELDOR, a pulse EPR method, to measure intramolecular distances |
| Q39012665 | Spinal cord regeneration in Xenopus laevis |
| Q48175408 | Split single-cell RT-PCR analysis of Purkinje cells |
| Q33286530 | Split-intein mediated circular ligation used in the synthesis of cyclic peptide libraries in E. coli. |
| Q90084119 | Stable isotope labeling of metal/metal oxide nanomaterials for environmental and biological tracing |
| Q114873432 | Stable isotope tracing to assess tumor metabolism in vivo |
| Q48218745 | Staining protocol for organotypic hippocampal slice cultures |
| Q92887098 | Standardization of complex biologically derived spectrochemical datasets |
| Q56534897 | Standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes |
| Q97692976 | Standardized bacteriophage purification for personalized phage therapy |
| Q48170795 | Standardized in vivo model for studying novel regenerative approaches for multitissue bone-ligament interfaces |
| Q33280963 | Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly. |
| Q80092968 | State-of-the-art two-dimensional gel electrophoresis: a key tool of proteomics research |
| Q27310361 | Statistical analysis of cell migration in 3D using the anisotropic persistent random walk model |
| Q34607711 | Stepwise differentiation of pluripotent stem cells into retinal cells |
| Q86449049 | Stereodefined trisubstituted enolates as a unique entry to all-carbon quaternary stereogenic centers in acyclic systems |
| Q48218703 | Stereotaxic gene delivery in the rodent brain |
| Q37354717 | Stiffness-controlled three-dimensional extracellular matrices for high-resolution imaging of cell behavior |
| Q30415094 | Stochastic profiling of transcriptional regulatory heterogeneities in tissues, tumors and cultured cells |
| Q36260798 | Strain Library Imaging Protocol for high-throughput, automated single-cell microscopy of large bacterial collections arrayed on multiwell plates |
| Q38838629 | Strategic and practical guidelines for successful structured illumination microscopy |
| Q53561762 | Strategies for site-specific protein biotinylation using in vitro, in vivo and cell-free systems: toward functional protein arrays. |
| Q45715896 | Stretching polysaccharides on live cells using single molecule force spectroscopy |
| Q51984227 | Stripe assay to examine axonal guidance and cell migration. |
| Q40150055 | Stromal cell-dependent growth of B-1 B cell progenitors in the absence of direct contact. |
| Q47650166 | Structural analysis of N- and O-glycans released from glycoproteins. |
| Q45385611 | Structural and functional analysis of single neurons to correlate synaptic connectivity with grooming behavior |
| Q57810986 | Structural interpretation of DNA-protein hydroxyl-radical footprinting experiments with high resolution using HYDROID |
| Q50107547 | Structural prediction of protein models using distance restraints derived from cross-linking mass spectrometry data. |
| Q91595678 | Structure determination protocol for transmembrane domain oligomers |
| Q33314548 | Study and selection of in vivo protein interactions by coupling bimolecular fluorescence complementation and flow cytometry. |
| Q40786144 | Study of in vivo catheter biofilm infections using pediatric central venous catheter implanted in rat. |
| Q30534784 | Studying calcium-triggered vesicle fusion in a single vesicle-vesicle content and lipid-mixing system |
| Q82502158 | Studying cell behavior in whole zebrafish embryos by confocal live imaging: application to hematopoietic stem cells |
| Q86892039 | Studying extracellular vesicle transfer by a Cre-loxP method |
| Q54471240 | Studying physical chromatin interactions in plants using Chromosome Conformation Capture (3C). |
| Q36049145 | Studying primary tumor-associated fibroblast involvement in cancer metastasis in mice |
| Q57612069 | Studying properties of neurotransmitter receptors by non-stationary noise analysis of spontaneous postsynaptic currents and agonist-evoked responses in outside-out patches |
| Q30541526 | Studying synapses in human brain with array tomography and electron microscopy |
| Q56772713 | Studying task-related activity of individual neurons in the human brain |
| Q39805297 | Studying the biodistribution of positron emission tomography reporter probes in mice |
| Q59662712 | Studying the uptake of cell-penetrating peptides |
| Q52818460 | Studying tumor growth in Drosophila using the tissue allograft method. |
| Q33280980 | Studying vesicle cycling in presynaptic terminals using the genetically encoded probe synaptopHluorin |
| Q30542861 | Sub-millisecond ligand probing of cell receptors with multiple solution exchange |
| Q45832104 | Subcellular calcium measurements in mammalian cells using jellyfish photoprotein aequorin-based probes. |
| Q40150108 | Subcellular imaging in the live mouse |
| Q33280985 | Substrate activity screening (SAS): a general procedure for the preparation and screening of a fragment-based non-peptidic protease substrate library for inhibitor discovery |
| Q33348945 | Substrate-induced gene expression (SIGEX) screening of metagenome libraries |
| Q33310276 | Subtractive single-chain antibody (scFv) phage-display: tailoring phage-display for high specificity against function-specific conformations of cell membrane molecules |
| Q95321757 | Subzero non-frozen preservation of human livers in the supercooled state |
| Q89486192 | Sucrose preference test for measurement of stress-induced anhedonia in mice |
| Q51032435 | Sulforhodamine B colorimetric assay for cytotoxicity screening. |
| Q38288107 | Super-resolution microscopy with DNA-PAINT. |
| Q35827783 | Supercooling preservation and transplantation of the rat liver |
| Q46322571 | Superoxide radical detection in cells, tissues, organisms (animals, plants, insects, microorganisms) and soils. |
| Q89687011 | Superresolution imaging of chromatin fibers to visualize epigenetic information on replicative DNA |
| Q35707982 | Superresolution live imaging of plant cells using structured illumination microscopy |
| Q85650450 | Surface analysis using shell-isolated nanoparticle-enhanced Raman spectroscopy |
| Q34382256 | Surface functionalization of barium titanate SHG nanoprobes for in vivo imaging in zebrafish |
| Q89486225 | Surface-wetting characterization using contact-angle measurements |
| Q37371721 | Surgical extraction of human dorsal root ganglia from organ donors and preparation of primary sensory neuron cultures |
| Q62065674 | Surgical implantation of an abdominal imaging window for intravital microscopy |
| Q37688522 | Surgical models for cardiac regeneration in neonatal mice |
| Q30372129 | Surgical models of Roux-en-Y gastric bypass surgery and sleeve gastrectomy in rats and mice. |
| Q33980608 | Synchronized fission yeast meiosis using an ATP analog-sensitive Pat1 protein kinase. |
| Q28267706 | Synchronous culture of Plasmodium falciparum at high parasitemia levels |
| Q37687859 | Synchronous infection of SIV and HIV in vitro for virology, immunology and vaccine-related studies |
| Q30654818 | Synchrotron-based small-angle X-ray scattering of proteins in solution |
| Q80078307 | Synthesis and application of an azobenzene amino acid as a light-switchable turn element in polypeptides |
| Q47598643 | Synthesis and characterization of well-defined hydrogel matrices and their application to intestinal stem cell and organoid culture. |
| Q46921645 | Synthesis and enzymatic incorporation of a fluorescent pyrimidine ribonucleotide |
| Q51032321 | Synthesis and oligonucleotide incorporation of fluorescent cytosine analogue tC: a promising nucleic acid probe. |
| Q87388353 | Synthesis and resolution of the biaryl-like diphosphine (S)-Me2-CATPHOS, preparation of a derived rhodium precatalyst and applications in asymmetric hydrogenation |
| Q44208965 | Synthesis and site-specific incorporation of a simple fluorescent pyrimidine |
| Q54484231 | Synthesis and use of QCy7-derived modular probes for the detection and imaging of biologically relevant analytes. |
| Q104073810 | Synthesis and use of an amphiphilic dendrimer for siRNA delivery into primary immune cells |
| Q53643259 | Synthesis and use of an asymmetric transfer hydrogenation catalyst based on iron(II) for the synthesis of enantioenriched alcohols and amines. |
| Q46789524 | Synthesis and use of an in-solution ratiometric fluorescent viscosity sensor |
| Q80422713 | Synthesis and utilization of reversible and irreversible light-activated nanovalves derived from the channel protein MscL |
| Q53490401 | Synthesis of 3,3'-bis(sulfonato)-4,4'-bis(chloroacetamido)azobenzene and cysteine cross-linking for photo-control of protein conformation and activity. |
| Q46845218 | Synthesis of 4-acetamido-2,2,6,6-tetramethylpiperidine-1-oxoammonium tetrafluoroborate and 4-acetamido-(2,2,6,6-tetramethyl-piperidin-1-yl)oxyl and their use in oxidative reactions |
| Q85913721 | Synthesis of 9-amino(9-deoxy)epi cinchona alkaloids, general chiral organocatalysts for the stereoselective functionalization of carbonyl compounds |
| Q57976151 | Synthesis of Dynole 34-2, Dynole 2-24 and Dyngo 4a for investigating dynamin GTPase |
| Q59666370 | Synthesis of N-heterocyclic carbene ligands and derived ruthenium olefin metathesis catalysts |
| Q83417203 | Synthesis of N-methylated cyclic peptides |
| Q80285824 | Synthesis of N-succinimidyl 4-[18F]fluorobenzoate, an agent for labeling proteins and peptides with 18F |
| Q80093450 | Synthesis of N-succinimidyl 4-guanidinomethyl-3-[*I]iodobenzoate: a radio-iodination agent for labeling internalizing proteins and peptides |
| Q80285913 | Synthesis of S-adenosyl-L-methionine analogs and their use for sequence-specific transalkylation of DNA by methyltransferases |
| Q80078259 | Synthesis of TOAC spin-labeled proteins and reconstitution in lipid membranes |
| Q48349166 | Synthesis of [(18)F]SiFB: a prosthetic group for direct protein radiolabeling for application in positron emission tomography. |
| Q41933022 | Synthesis of a HyCoSuL peptide substrate library to dissect protease substrate specificity |
| Q33280977 | Synthesis of a biocleavable polyrotaxane-plasmid DNA (pDNA) polyplex and its use for the rapid nonviral delivery of pDNA to cell nuclei |
| Q46896539 | Synthesis of a fluorescent analogue of geranylgeranyl pyrophosphate and its use in a high-throughput fluorometric assay for Rab geranylgeranyltransferase |
| Q46535136 | Synthesis of a highly HOCl-selective fluorescent probe and its use for imaging HOCl in cells and organisms |
| Q38718390 | Synthesis of a highly Mg2+-selective fluorescent probe and its application to quantifying and imaging total intracellular magnesium |
| Q87787982 | Synthesis of a highly Zn(2+)-selective cyanine-based probe and its use for tracing endogenous zinc ions in cells and organisms |
| Q50953444 | Synthesis of a highly metal-selective rhodamine-based probe and its use for the in vivo monitoring of mercury. |
| Q80286043 | Synthesis of a multivalent chelator lipid for stably tethering histidine-tagged proteins onto membranes |
| Q37407980 | Synthesis of a probe for monitoring HSV1-tk reporter gene expression using chemical exchange saturation transfer MRI. |
| Q43079085 | Synthesis of alkyl- and aryl-amino-substituted anthraquinone derivatives by microwave-assisted copper(0)-catalyzed Ullmann coupling reactions |
| Q84261511 | Synthesis of all-hydrocarbon stapled α-helical peptides by ring-closing olefin metathesis |
| Q87388350 | Synthesis of an electron-rich KITPHOS monophosphine, preparation of derived metal complexes and applications in catalysis |
| Q91705472 | Synthesis of an ultrasensitive BODIPY-derived fluorescent probe for detecting HOCl in live cells |
| Q46850566 | Synthesis of anhydride precursors of the environment-sensitive fluorophores 4-DMAP and 6-DMN. |
| Q51033607 | Synthesis of azobenzene-tethered DNA for reversible photo-regulation of DNA functions: hybridization and transcription. |
| Q39735679 | Synthesis of bicyclo[5.3.0]azulene derivatives |
| Q46260753 | Synthesis of biotin-AMP conjugate for 5' biotin labeling of RNA through one-step in vitro transcription |
| Q51009460 | Synthesis of cationic conjugated polymers for use in label-free DNA microarrays. |
| Q46850575 | Synthesis of cationic single-isomer cyclodextrins for the chiral separation of amino acids and anionic pharmaceuticals |
| Q37492590 | Synthesis of complex benzenoids via the intermediate generation of o-benzynes through the hexadehydro-Diels-Alder reaction. |
| Q52847711 | Synthesis of complex head-to-side-chain cyclodepsipeptides. |
| Q46809744 | Synthesis of enantiomerically enriched (R)-5-tert-butylazepan-2-one using a hydroxyalkyl azide mediated ring-expansion reaction |
| Q34989218 | Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ |
| Q57362923 | Synthesis of functionalized amphiphilic polymers for coating quantum dots |
| Q91740209 | Synthesis of high-molar-activity [18F]6-fluoro-L-DOPA suitable for human use via Cu-mediated fluorination of a BPin precursor |
| Q46941959 | Synthesis of lactones using substituted benzoic anhydride as a coupling reagent |
| Q80093403 | Synthesis of light-activated antisense oligodeoxynucleotide |
| Q38702089 | Synthesis of magnetic resonance-, X-ray- and ultrasound-visible alginate microcapsules for immunoisolation and noninvasive imaging of cellular therapeutics |
| Q51032374 | Synthesis of non-natural ManNAc analogs for the expression of thiols on cell-surface sialic acids. |
| Q36443834 | Synthesis of oligo(poly(ethylene glycol) fumarate). |
| Q33286533 | Synthesis of oligo-RNAs with photocaged adenosine 2'-hydroxyls |
| Q45195236 | Synthesis of orthogonally protected bacterial, rare-sugar and D-glycosamine building blocks |
| Q42844150 | Synthesis of peptide macrocycles using unprotected amino aldehydes |
| Q33762066 | Synthesis of photodegradable hydrogels as dynamically tunable cell culture platforms |
| Q46896527 | Synthesis of poly(L-lactide) and polyglycolide by ring-opening polymerization |
| Q34792676 | Synthesis of poly(propylene fumarate). |
| Q64447366 | Synthesis of polystyrene microspheres and functionalization with Pd0 nanoparticles to perform bioorthogonal organometallic chemistry in living cells |
| Q80805173 | Synthesis of pyrimidines by direct condensation of amides and nitriles |
| Q46850582 | Synthesis of the 3'-thio-nucleosides and subsequent automated synthesis of oligodeoxynucleotides containing a 3'-S-phosphorothiolate linkage. |
| Q56973126 | Synthesis of the Pitstop family of clathrin inhibitors |
| Q46202125 | Synthesis of the functionalizable methionine surrogate azidohomoalanine using Boc-homoserine as precursor |
| Q45947770 | Synthesis of α-allylated α,β-unsaturated carbonyl compounds using vanadium/palladium contemporaneous dual catalysis. |
| Q46941957 | Synthesis, encapsulation, purification and coupling of single quantum dots in phospholipid micelles for their use in cellular and in vivo imaging. |
| Q35151845 | Synthesis, labeling and bioanalytical applications of a tris(2,2'-bipyridyl)ruthenium(II)-based electrochemiluminescence probe. |
| Q52847708 | Synthesis, lipid membrane incorporation, and ion permeability testing of carbon nanotube porins. |
| Q80188275 | Synthesis, purification and sample experiment for fluorescent pteridine-containing DNA: tools for studying DNA interactive systems |
| Q92565020 | Synthetic chemistry in water: applications to peptide synthesis and nitro-group reductions |
| Q44998388 | System-wide identification of RNA-binding proteins by interactome capture. |
| Q27860739 | Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources |
| Q48100003 | Systematic assessment of training-induced changes in corticospinal output to hand using frameless stereotaxic transcranial magnetic stimulation |
| Q90101533 | Systematic prediction of functionally linked genes in bacterial and archaeal genomes |
| Q36846036 | Systematic prediction of gene function in Arabidopsis thaliana using a probabilistic functional gene network |
| Q90971001 | Systemic AAV vectors for widespread and targeted gene delivery in rodents |
| Q91595637 | Systems structural biology measurements by in vivo cross-linking with mass spectrometry |
| Q92548346 | Systems-level analysis of isotopic labeling in untargeted metabolomic data by X13CMS |
| Q29247919 | T-REX on-demand redox targeting in live cells. |
| Q48218933 | T-maze alternation in the rodent |
| Q35826318 | TARDIS, a targeted RNA directional sequencing method for rare RNA discovery |
| Q54443867 | Tagging recombinant proteins with a Sel-tag for purification, labeling with electrophilic compounds or radiolabeling with 11C. |
| Q35000402 | Tagmentation-based whole-genome bisulfite sequencing |
| Q41888062 | Tandem affinity purification of functional TAP-tagged proteins from human cells |
| Q46746488 | Tandem orthogonal proteolysis-activity-based protein profiling (TOP-ABPP)--a general method for mapping sites of probe modification in proteomes |
| Q30699563 | Target analysis by integration of transcriptome and ChIP-seq data with BETA. |
| Q58883601 | Target-enrichment sequencing for detailed characterization of small RNAs |
| Q48486925 | Targeted axon-attached recording with fluorescent patch-clamp pipettes in brain slices. |
| Q48218905 | Targeted bulk-loading of fluorescent indicators for two-photon brain imaging in vivo |
| Q52719687 | Targeted in situ genome-wide profiling with high efficiency for low cell numbers. |
| Q48246545 | Targeted in vivo genetic manipulation of the mouse or rat brain by in utero electroporation with a triple-electrode probe. |
| Q90894134 | Targeted intraspinal injections to assess therapies in rodent models of neurological disorders |
| Q40704645 | Targeted isolation and cloning of 100-kb microbial genomic sequences by Cas9-assisted targeting of chromosome segments |
| Q42926067 | Targeted mutagenesis in zebrafish using customized zinc-finger nucleases |
| Q34097122 | Targeted optogenetic stimulation and recording of neurons in vivo using cell-type-specific expression of Channelrhodopsin-2. |
| Q48320125 | Targeted sequencing for gene discovery and quantification using RNA CaptureSeq. |
| Q48614885 | Targeted single-cell electroporation of mammalian neurons in vivo. |
| Q43122068 | Targeting mammalian organelles with internalizing phage (iPhage) libraries |
| Q92565014 | Temperature imaging using a cationic linear fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy |
| Q46123430 | Temperature-dependent FRET spectroscopy for the high-throughput analysis of self-assembled DNA nanostructures in real time |
| Q30419445 | Template-based protein structure modeling using the RaptorX web server. |
| Q87787099 | Ten years of Nature Protocols |
| Q50998179 | Testing declarative memory in laboratory rats and mice using the nonconditioned social discrimination procedure. |
| Q34034581 | Tet-assisted bisulfite sequencing of 5-hydroxymethylcytosine. |
| Q38304840 | Tethered particle analysis of supercoiled circular DNA using peptide nucleic acid handles |
| Q40005181 | Tetrazolium-based colorimetric assay for the detection of HIV replication inhibitors: revisited 20 years later |
| Q80092878 | The 'reverse capture' autoantibody microarray: a native antigen-based platform for autoantibody profiling |
| Q61774264 | The 32P-postlabeling assay for DNA adducts |
| Q33310277 | The Bead blot: a method for selecting small molecule ligands for protein capture and purification |
| Q39024817 | The ClusPro web server for protein-protein docking |
| Q50313919 | The Dimensional Change Card Sort (DCCS): a method of assessing executive function in children. |
| Q36604792 | The FTMap family of web servers for determining and characterizing ligand-binding hot spots of proteins |
| Q30448765 | The GLIB technique for genome-wide mapping of 5-hydroxymethylcytosine |
| Q29617755 | The HADDOCK web server for data-driven biomolecular docking |
| Q91740215 | The HDOCK server for integrated protein-protein docking |
| Q51551911 | The INTACT method for cell type-specific gene expression and chromatin profiling in Arabidopsis thaliana. |
| Q80092940 | The JAM Test and its daughter P-JAM: simple tests of DNA fragmentation to measure cell death and stasis |
| Q39220830 | The MaxQuant computational platform for mass spectrometry-based shotgun proteomics |
| Q38956347 | The Mini-FLOTAC technique for the diagnosis of helminth and protozoan infections in humans and animals |
| Q48218913 | The Multi-Source Interference Task: an fMRI task that reliably activates the cingulo-frontal-parietal cognitive/attention network |
| Q85896311 | The PURE system for the cell-free synthesis of membrane proteins |
| Q29616136 | The Phyre2 web portal for protein modeling, prediction and analysis |
| Q42122474 | The SCX/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry |
| Q34004711 | The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins |
| Q48444463 | The adhesive removal test: a sensitive method to assess sensorimotor deficits in mice. |
| Q30448762 | The anti-CMS technique for genome-wide mapping of 5-hydroxymethylcytosine |
| Q64218040 | The application of the 5-choice serial reaction time task for the assessment of visual attentional processes and impulse control in rats |
| Q50089005 | The assembly and use of continuous flow systems for chemical synthesis. |
| Q80092850 | The bio-barcode assay for the detection of protein and nucleic acid targets using DTT-induced ligand exchange |
| Q34432745 | The cellular thermal shift assay for evaluating drug target interactions in cells |
| Q58905313 | The chemoinvasion assay: a method to assess tumor and endothelial cell invasion and its modulation |
| Q35989421 | The collection of MicroED data for macromolecular crystallography |
| Q64388028 | The comet assay: a method to measure DNA damage in individual cells |
| Q38398231 | The counting Stroop: a cognitive interference task |
| Q45071864 | The cubicon method for concentrating membrane proteins in the cubic mesophase |
| Q80805170 | The detection and quantification of growth cone collapsing activities |
| Q30544122 | The embryonic mouse hindbrain as a qualitative and quantitative model for studying the molecular and cellular mechanisms of angiogenesis |
| Q48218920 | The emotional counting Stroop: a task for assessing emotional interference during brain imaging |
| Q48138480 | The fetal mouse metatarsal bone explant as a model of angiogenesis. |
| Q50690888 | The fluorescence protease protection (FPP) assay to determine protein localization and membrane topology. |
| Q39949051 | The fluorometric microculture cytotoxicity assay |
| Q51032516 | The gelatin sponge-chorioallantoic membrane assay. |
| Q30318744 | The generation of hepatocytes from mesenchymal stem cells and engraftment into murine liver |
| Q45168680 | The generation of kidney organoids by differentiation of human pluripotent cells to ureteric bud progenitor-like cells |
| Q41877949 | The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells. |
| Q48122459 | The isolation and culture of endothelial colony-forming cells from human and rat lungs |
| Q90497532 | The isolation and molecular characterization of cerebral microvessels |
| Q33568518 | The learned safety paradigm as a mouse model for neuropsychiatric research |
| Q50690805 | The lineaging of fluorescently-labeled Caenorhabditis elegans embryos with StarryNite and AceTree. |
| Q114873109 | The maternal serum metabolome by multisegment injection-capillary electrophoresis-mass spectrometry: a high-throughput platform and standardized data workflow for large-scale epidemiological studies |
| Q51032367 | The mode-of-action by network identification (MNI) algorithm: a network biology approach for molecular target identification. |
| Q59123131 | The morphological and molecular features of the epithelial-to-mesenchymal transition |
| Q46941937 | The mouse cornea micropocket angiogenesis assay |
| Q44804631 | The mouse excisional wound splinting model, including applications for stem cell transplantation |
| Q80285942 | The multiple-choice formats (forms F and G) of the Benton Visual Retention Test as a tool to detect age-related memory changes in population-based studies and clinical settings |
| Q59338493 | The parallel rod floor test: a measure of ataxia in mice |
| Q80805149 | The partial reduction of electron-deficient pyrroles: procedures describing both Birch (Li/NH3) and ammonia-free (Li/DBB) conditions |
| Q58746933 | The past, present and future of microbiome analyses |
| Q38825473 | The power of multiplexed functional analysis of genetic variants. |
| Q36160925 | The preparation and development of cellular membrane affinity chromatography columns |
| Q46388227 | The preparation of site-specifically modified riboswitch domains as an example for enzymatic ligation of chemically synthesized RNA fragments. |
| Q57083634 | The prion organotypic slice culture assay—POSCA |
| Q40508335 | The real-time quaking-induced conversion assay for detection of human prion disease and study of other protein misfolding diseases |
| Q50875905 | The regular and light-dark Suok tests of anxiety and sensorimotor integration: utility for behavioral characterization in laboratory rodents. |
| Q24634193 | The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments |
| Q37413964 | The role of IFN-gamma Elispot assay in HIV vaccine research |
| Q46554007 | The selective detection of mitochondrial superoxide by live cell imaging |
| Q28296342 | The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on |
| Q88220517 | The successes and future prospects of the linear antisense RNA amplification methodology |
| Q45092393 | The synthesis of tetra-modified RNA for the multidimensional control of gene expression via light-activated RNA interference. |
| Q80078311 | The tagged microarray marker (TAM) method for high-throughput detection of single nucleotide and indel polymorphisms |
| Q34040442 | The touchscreen operant platform for assessing executive function in rats and mice |
| Q30570928 | The touchscreen operant platform for testing learning and memory in rats and mice |
| Q37695412 | The touchscreen operant platform for testing working memory and pattern separation in rats and mice. |
| Q29547634 | The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability |
| Q43544530 | The use of mesenchymal stem cells in collagen-based scaffolds for tissue-engineered repair of tendons |
| Q43090031 | The use of phage display to generate conformation-sensor recombinant antibodies |
| Q90836379 | The use of sonicated lipid vesicles for mass spectrometry of membrane protein complexes |
| Q80093506 | The use of the Ugi four-component condensation |
| Q36759180 | The use of the elevated plus maze as an assay of anxiety-related behavior in rodents |
| Q42808743 | The validated embryonic stem cell test to predict embryotoxicity in vitro |
| Q46896517 | The zebrafish/tumor xenograft angiogenesis assay. |
| Q80093415 | Thermal and high pressure intramolecular Diels-Alder reaction of vinylsulfonamides |
| Q35778631 | Thermal proteome profiling for unbiased identification of direct and indirect drug targets using multiplexed quantitative mass spectrometry. |
| Q37030421 | Thermodynamic analysis of protein-ligand binding interactions in complex biological mixtures using the stability of proteins from rates of oxidation |
| Q27310442 | Thinned-skull cranial window technique for long-term imaging of the cortex in live mice |
| Q43071305 | Thiol-based, site-specific and covalent immobilization of biomolecules for single-molecule experiments |
| Q85752448 | Three-dimensional cell culturing by magnetic levitation |
| Q34444100 | Three-dimensional imaging of solvent-cleared organs using 3DISCO. |
| Q34060757 | Three-dimensional lithographically defined organotypic tissue arrays for quantitative analysis of morphogenesis and neoplastic progression |
| Q44031885 | Time-lapse X-ray phase-contrast microtomography for in vivo imaging and analysis of morphogenesis. |
| Q33280970 | Time-lapse imaging of dendritic spines in vitro |
| Q37108809 | Time-resolved RNA SHAPE chemistry: quantitative RNA structure analysis in one-second snapshots and at single-nucleotide resolution |
| Q46520207 | Time-resolved fluorescence ligand binding for G protein-coupled receptors |
| Q80285905 | Tissue subcellular fractionation and protein extraction for use in mass-spectrometry-based proteomics |
| Q48225480 | Tissue-engineered 3D human lymphatic microvascular network for in vitro studies of lymphangiogenesis. |
| Q80093210 | Tissue-specific and cell type-specific RNA interference in vivo |
| Q57887340 | Titanium dioxide as a chemo-affinity solid phase in offline phosphopeptide chromatography prior to HPLC-MS/MS analysis |
| Q34008445 | Titration-free 454 sequencing using Y adapters |
| Q42214294 | Tobacco rattle virus-based virus-induced gene silencing in Nicotiana benthamiana. |
| Q104111445 | Tools for experimental and computational analyses of off-target editing by programmable nucleases |
| Q46850569 | Tools for investigating peptide-protein interactions: peptide incorporation of environment-sensitive fluorophores through SPPS-based 'building block' approach. |
| Q46850572 | Tools for investigating peptide-protein interactions: peptide incorporation of environment-sensitive fluorophores via on-resin derivatization. |
| Q30414346 | Topological analysis and interactive visualization of biological networks and protein structures |
| Q47693387 | Total chemical synthesis of histones and their analogs, assisted by native chemical ligation and palladium complexes |
| Q42511427 | Total internal reflection with fluorescence correlation spectroscopy |
| Q42872339 | Total low-molecular-weight antioxidants as a summary parameter, quantified in biological samples by a chemiluminescence inhibition assay |
| Q43811300 | Total synthesis of multi-kilobase DNA sequences from oligonucleotides |
| Q33366975 | Touchdown PCR for increased specificity and sensitivity in PCR amplification |
| Q90591817 | Toward a unified framework for interpreting machine-learning models in neuroimaging |
| Q48280933 | Tracheal tissue engineering in rats. |
| Q112714872 | Tracing DNA paths and RNA profiles in cultured cells and tissues with ORCA |
| Q104111446 | Tracking endocytosis and intracellular distribution of spherical nucleic acids with correlative single-cell imaging |
| Q36454999 | Tracking epitope-specific T cells. |
| Q34664023 | Tracking intracellular protein movements using photoswitchable fluorescent proteins PS-CFP2 and Dendra2. |
| Q47333860 | Tracking mechanics and volume of globular cells with atomic force microscopy using a constant-height clamp |
| Q42272002 | Tracking recent adaptive evolution in microbial species using TimeZone |
| Q34263160 | Tracking the sources of blood meals of parasitic arthropods using shotgun proteomics and unidentified tandem mass spectral libraries |
| Q37275276 | Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown |
| Q34066561 | Transcription factor binding predictions using TRAP for the analysis of ChIP-seq data and regulatory SNPs |
| Q112721373 | Transcription factor chromatin profiling genome-wide using uliCUT&RUN in single cells and individual blastocysts |
| Q35598003 | Transcriptome-wide identification of adenosine-to-inosine editing using the ICE-seq method. |
| Q28828753 | Transcriptome-wide interrogation of RNA secondary structure in living cells with icSHAPE |
| Q51534536 | Transcriptome-wide mapping of N(6)-methyladenosine by m(6)A-seq based on immunocapturing and massively parallel sequencing. |
| Q87113993 | Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP |
| Q52706811 | Transcuticular optical imaging of stimulus-evoked neural activities in the Drosophila peripheral nervous system. |
| Q51766318 | Transfection of cells using flow-through electroporation based on constant voltage. |
| Q40135666 | Transfection of mammalian cells with connexins and measurement of voltage sensitivity of their gap junctions. |
| Q84255216 | Transfection of rat or mouse neurons by biolistics or electroporation |
| Q46566932 | Transferring whole genomes from bacteria to yeast spheroplasts using entire bacterial cells to reduce DNA shearing |
| Q52579554 | Transformation of Dictyostelium discoideum with plasmid DNA. |
| Q37670085 | Transformation of the rodent malaria parasite Plasmodium chabaudi |
| Q92503300 | Transgenesis in Hydra to characterize gene function and visualize cell behavior |
| Q50690822 | Transgenesis in fish: efficient selection of transgenic fish by co-injection with a fluorescent reporter construct. |
| Q50524860 | Transient expression and analysis of fluorescent reporter proteins in plant pollen tubes. |
| Q50882347 | Transient expression of fluorescent fusion proteins in protoplasts of suspension cultured cells. |
| Q47249953 | Transient expression of human antibodies in mammalian cells. |
| Q33513818 | Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies |
| Q36295149 | Translation complex profile sequencing to study the in vivo dynamics of mRNA-ribosome interactions during translation initiation, elongation and termination |
| Q51839435 | Transposon-mediated BAC transgenesis in zebrafish. |
| Q34615345 | Tricine-SDS-PAGE. |
| Q80093289 | Triflimide (HNTf2)-catalyzed aldehyde cross-aldol reaction using "super silyl" enol ethers |
| Q41900596 | Triggering cell detachment from patterned electrode arrays by programmed subcellular release. |
| Q92097395 | Tumor organoid-T-cell coculture systems |
| Q46131292 | Tunable pharmacokinetics: modifying the in vivo half-life of antibodies by directed mutagenesis of the Fc fragment |
| Q38984575 | Turning single-molecule localization microscopy into a quantitative bioanalytical tool |
| Q100316472 | Tutorial: a computational framework for the design and optimization of peripheral neural interfaces |
| Q97692972 | Tutorial: a guide to performing polygenic risk score analyses |
| Q98184354 | Tutorial: avoiding and correcting sample-induced spherical aberration artifacts in 3D fluorescence microscopy |
| Q100958934 | Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays |
| Q90814952 | Tutorial: guidance for quantitative confocal microscopy |
| Q107158224 | Tutorial: guidelines for annotating single-cell transcriptomic maps using automated and manual methods |
| Q100737231 | Tutorial: guidelines for standardized performance tests for electrodes intended for neural interfaces and bioelectronics |
| Q104111447 | Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data |
| Q62780607 | Tutorial: guidelines for the experimental design of single-cell RNA sequencing studies |
| Q96577265 | Tutorial: multivariate classification for vibrational spectroscopy in biological samples |
| Q98952123 | Tutorial: structural characterization of isolated metal atoms and subnanometric metal clusters in zeolites |
| Q48401972 | Two-day radial-arm water maze learning and memory task; robust resolution of amyloid-related memory deficits in transgenic mice. |
| Q39792303 | Two-dimensional difference gel electrophoresis |
| Q41137577 | Two-dimensional fluorescence difference gel electrophoresis for comparative proteomics profiling |
| Q33294340 | Two-dimensional gel electrophoresis for identifying proteins that bind DNA or RNA. |
| Q80093398 | Two-dimensional strandness-dependent electrophoresis |
| Q33280946 | Two-photon targeted patching (TPTP) in vivo |
| Q33623334 | Two-photon targeted recording of GFP-expressing neurons for light responses and live-cell imaging in the mouse retina |
| Q53561789 | Two-step metal affinity purification of double-tagged (NusA-His6) fusion proteins. |
| Q33987948 | Two-way communication with neural networks in vivo using focused light |
| Q38811002 | Ultra-fast, label-free isolation of circulating tumor cells from blood using spiral microfluidics |
| Q40024977 | Ultra-high-throughput sequencing of the immune receptor repertoire from millions of lymphocytes |
| Q48921761 | Ultrasonic neuromodulation by brain stimulation with transcranial ultrasound. |
| Q62033967 | Ultrastructural readout of functional synaptic vesicle pools in hippocampal slices based on FM dye labeling and photoconversion |
| Q80643388 | Unbiased identification of cysteine S-nitrosylation sites on proteins |
| Q53283432 | Unbiased quantification of immunoglobulin diversity at the DNA level with VDJ-seq. |
| Q125943864 | Uncovering structural ensembles from single-particle cryo-EM data using cryoDRGN |
| Q92564931 | Understanding chemical reactivity using the activation strain model |
| Q35220901 | Unique nucleotide sequence-guided assembly of repetitive DNA parts for synthetic biology applications |
| Q38356146 | Universal protein-binding microarrays for the comprehensive characterization of the DNA-binding specificities of transcription factors |
| Q51477274 | Universal sample preparation method integrating trichloroacetic acid/acetone precipitation with phenol extraction for crop proteomic analysis. |
| Q92503882 | Unraveling human adult hippocampal neurogenesis |
| Q46083073 | Unrestrictive identification of post-translational modifications through peptide mass spectrometry |
| Q58889396 | Untargeted large-scale plant metabolomics using liquid chromatography coupled to mass spectrometry |
| Q35000405 | Upconversion luminescence imaging of cells and small animals |
| Q80093523 | Uptake and release protocol for assessing membrane binding and permeation by way of isothermal titration calorimetry |
| Q46809760 | Use of Raman spectroscopy as a tool for in situ monitoring of microwave-promoted reactions |
| Q47294183 | Use of TAI-FISH to visualize neural ensembles activated by multiple stimuli |
| Q42060671 | Use of a commercially available reagent for the selective detection of homocysteine in plasma |
| Q45174579 | Use of a lentivirus/VSV pseudotype virus for highly efficient genetic redirection of human peripheral blood lymphocytes |
| Q47705950 | Use of a neonatal rat system as a bioincubator to generate adult-like mature cardiomyocytes from human and mouse pluripotent stem cells. |
| Q48310539 | Use of a three-layer gradient system of cells for rat testicular organoid generation. |
| Q38644638 | Use of fluorescence-detected sedimentation velocity to study high-affinity protein interactions |
| Q81437855 | Use of high-performance anion exchange chromatography with pulsed amperometric detection for O-glycan determination in yeast |
| Q58568612 | Use of human induced pluripotent stem cell-derived cardiomyocytes to assess drug cardiotoxicity |
| Q100958923 | Use of human peripheral blood mononuclear cells to define immunological properties of nucleic acid nanoparticles |
| Q36189818 | Use of human tissue explants to study human infectious agents |
| Q39867620 | Use of in vivo biotinylation to study protein-protein and protein-DNA interactions in mouse embryonic stem cells |
| Q38697943 | Use of luciferase probes to measure ATP in living cells and animals |
| Q43168876 | Use of luminescent bacteria for rapid screening and characterization of short cationic antimicrobial peptides synthesized on cellulose using peptide array technology |
| Q83366737 | Use of stable isotope labeling by amino acids in cell culture as a spike-in standard in quantitative proteomics |
| Q112720538 | Use of stable isotope-tagged thymidine and multi-isotope imaging mass spectrometry (MIMS) for quantification of human cardiomyocyte division |
| Q92886057 | Use of synthetic DNA spike-in controls (sequins) for human genome sequencing |
| Q37189999 | Use of target protector morpholinos to analyze the physiological roles of specific miRNA-mRNA pairs in vivo |
| Q91705481 | Use of the iNo score to discriminate normal from altered nucleolar morphology, with applications in basic cell biology and potential in human disease diagnostics |
| Q58313759 | Use of the mouse aortic ring assay to study angiogenesis |
| Q50930928 | Use of the supported membrane tube assay system for real-time analysis of membrane fission reactions. |
| Q44462472 | Using 5-deoxy-5-[18F]fluororibose to glycosylate peptides for positron emission tomography |
| Q91011163 | Using BEAN-counter to quantify genetic interactions from multiplexed barcode sequencing experiments |
| Q91451039 | Using CAPTURE to detect spacer acquisition in native CRISPR arrays |
| Q92173285 | Using Cre-recombinase-driven Polylox barcoding for in vivo fate mapping in mice |
| Q92949062 | Using DeepLabCut for 3D markerless pose estimation across species and behaviors |
| Q30655657 | Using Fourier transform IR spectroscopy to analyze biological materials |
| Q114873073 | Using MetaboAnalyst 5.0 for LC–HRMS spectra processing, multi-omics integration and covariate adjustment of global metabolomics data |
| Q92669580 | Using MicrobiomeAnalyst for comprehensive statistical, functional, and meta-analysis of microbiome data |
| Q28286406 | Using ProtMAX to create high-mass-accuracy precursor alignments from label-free quantitative mass spectrometry data generated in shotgun proteomics experiments |
| Q38360727 | Using RSAT oligo-analysis and dyad-analysis tools to discover regulatory signals in nucleic sequences |
| Q38360732 | Using RSAT to scan genome sequences for transcription factor binding sites and cis-regulatory modules |
| Q39931839 | Using Raman spectroscopy to characterize biological materials |
| Q33637419 | Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria. |
| Q38997139 | Using TRIP for genome-wide position effect analysis in cultured cells |
| Q92503909 | Using TTchem-seq for profiling nascent transcription and measuring transcript elongation |
| Q51560425 | Using Trawler_standalone to discover overrepresented motifs in DNA and RNA sequences derived from various experiments including chromatin immunoprecipitation. |
| Q50670273 | Using an adherent cell culture of the mouse subependymal zone to study the behavior of adult neural stem cells on a single-cell level. |
| Q34213469 | Using bleach-chase to measure protein half-lives in living cells. |
| Q42566843 | Using circular dichroism collected as a function of temperature to determine the thermodynamics of protein unfolding and binding interactions |
| Q24650518 | Using circular dichroism spectra to estimate protein secondary structure |
| Q38969994 | Using connectome-based predictive modeling to predict individual behavior from brain connectivity |
| Q48608961 | Using drug-discrimination techniques to study the abuse-related effects of psychoactive drugs in rats. |
| Q63359409 | Using enhanced number and brightness to measure protein oligomerization dynamics in live cells |
| Q34030307 | Using formaldehyde-assisted isolation of regulatory elements (FAIRE) to isolate active regulatory DNA. |
| Q36288222 | Using hiCLIP to identify RNA duplexes that interact with a specific RNA-binding protein. |
| Q124516097 | Using high-resolution microscopy data to generate realistic structures for electromagnetic FDTD simulations from complex biological models |
| Q38370982 | Using hyperLOPIT to perform high-resolution mapping of the spatial proteome. |
| Q30601270 | Using injectoporation to deliver genes to mechanosensory hair cells |
| Q42186917 | Using mammalian GFP reconstitution across synaptic partners (mGRASP) to map synaptic connectivity in the mouse brain. |
| Q54289153 | Using multiplex single-base extension typing to screen for mutants defective in RNA editing. |
| Q48253337 | Using pancreas tissue slices for in situ studies of islet of Langerhans and acinar cell biology |
| Q30438988 | Using patterned supported lipid membranes to investigate the role of receptor organization in intercellular signaling |
| Q51032424 | Using phiC31 integrase to make transgenic Xenopus laevis embryos. |
| Q34255194 | Using probabilistic estimation of expression residuals (PEER) to obtain increased power and interpretability of gene expression analyses |
| Q30277319 | Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons |
| Q80093188 | Using tRNA-linked molecular beacons to image cytoplasmic mRNAs in live cells |
| Q30584300 | Using the Drosophila melanogaster D17-c3 cell culture system to study cell motility |
| Q50921125 | Using the GEMM-ESC strategy to study gene function in mouse models. |
| Q37695738 | Using the Q system in Drosophila melanogaster |
| Q42619198 | Using the T-Coffee package to build multiple sequence alignments of protein, RNA, DNA sequences and 3D structures |
| Q28248466 | Using the beta-lactamase protein-fragment complementation assay to probe dynamic protein-protein interactions |
| Q41128017 | Using the chicken embryo to assess virulence of Listeria monocytogenes and to model other microbial infections |
| Q52736521 | Using the mitochondria-targeted ratiometric mass spectrometry probe MitoB to measure H2O2 in living Drosophila. |
| Q57513608 | Using the rat forced swim test to assess antidepressant-like activity in rodents |
| Q91637218 | Using the tube test to measure social hierarchy in mice |
| Q42250783 | Using transmission electron microscopy and 3View to determine collagen fibril size and three-dimensional organization. |
| Q114109147 | Utilizing Skyline to analyze lipidomics data containing liquid chromatography, ion mobility spectrometry and mass spectrometry dimensions |
| Q90813196 | V2a interneuron differentiation from mouse and human pluripotent stem cells |
| Q30357072 | Validation of metal-binding sites in macromolecular structures with the CheckMyMetal web server. |
| Q35133307 | Variable gelation time and stiffness of low-molecular-weight hydrogels through catalytic control over self-assembly |
| Q33640631 | Verification of automated peptide identifications from proteomic tandem mass spectra |
| Q80422708 | Versatile synthesis of oligodeoxyribonucleotide-oligospermine conjugates |
| Q27860583 | Version 1.2 of the Crystallography and NMR system |
| Q30512199 | Vertebrate neural stem cell segmentation, tracking and lineaging with validation and editing |
| Q41609059 | Vibrational spectroscopic image analysis of biological material using multivariate curve resolution-alternating least squares (MCR-ALS). |
| Q47845049 | Video tracking and analysis of sleep in Drosophila melanogaster |
| Q103836841 | Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes |
| Q86660202 | Virtual karyotyping of pluripotent stem cells on the basis of their global gene expression profiles |
| Q45405579 | Virus and prokaryote enumeration from planktonic aquatic environments by epifluorescence microscopy with SYBR Green I. |
| Q31108504 | Visualization and cellular hierarchy inference of single-cell data using SPADE. |
| Q50543108 | Visualization and genetic analysis of alternative splicing regulation in vivo using fluorescence reporters in transgenic Caenorhabditis elegans |
| Q42829548 | Visualization of F-actin and G-actin equilibrium using fluorescence resonance energy transfer (FRET) in cultured cells and neurons in slices. |
| Q37588185 | Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lenses. |
| Q84106665 | Visualization of gene expression in whole mouse retina by in situ hybridization |
| Q33319841 | Visualization of macromolecular complexes using cryo-electron microscopy with FEI Tecnai transmission electron microscopes |
| Q46499589 | Visualization of protein interactions in living Caenorhabditis elegans using bimolecular fluorescence complementation analysis |
| Q39786892 | Visualization of small GTPase activity with fluorescence resonance energy transfer-based biosensors |
| Q34657354 | Visualization of ternary complexes in living cells by using a BiFC-based FRET assay |
| Q40124495 | Visualization of the nucleus and nuclear envelope in situ by SEM in tissue culture cells. |
| Q47299598 | Visualizing endocytic recycling and trafficking in live neurons by subdiffractional tracking of internalized molecules |
| Q95832005 | Visualizing the functional 3D shape and topography of long noncoding RNAs by single-particle atomic force microscopy and in-solution hydrodynamic techniques |
| Q84255206 | Voltage-clamp and current-clamp recordings from mammalian DRG neurons |
| Q51550182 | Volumetric real-time multispectral optoacoustic tomography of biomarkers. |
| Q90617738 | Water-assisted laser desorption/ionization mass spectrometry for minimally invasive in vivo and real-time surface analysis using SpiderMass |
| Q28746102 | Web-based inference of biological patterns, functions and pathways from metabolomic data using MetaboAnalyst |
| Q45816501 | Whole plant cell wall characterization using solution-state 2D NMR. |
| Q46929905 | Whole-body imaging of bacterial infection and antibiotic response |
| Q46062699 | Whole-body imaging with fluorescent proteins |
| Q37029369 | Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping |
| Q92955689 | Whole-brain block-face serial microscopy tomography at subcellular resolution using FAST |
| Q34632731 | Whole-genome chromatin profiling from limited numbers of cells using nano-ChIP-seq |
| Q34626413 | Whole-genome multiple displacement amplification from single cells |
| Q46671700 | Whole-mount immunolocalization to study female meiosis in Arabidopsis. |
| Q50468909 | Whole-mount immunostaining of Drosophila skeletal muscle. |
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