An in vivo RNAi assay identifies major genetic and cellular requirements for primary piRNA biogenesis in Drosophila

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An in vivo RNAi assay identifies major genetic and cellular requirements for primary piRNA biogenesis in Drosophila is …
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scholarly articleQ13442814

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P356DOI10.1038/EMBOJ.2010.212
P932PMC publication ID2957214
P698PubMed publication ID20818334
P5875ResearchGate publication ID46149697

P50authorKarl MechtlerQ28320558
Julius BrenneckeQ38154184
P2093author name stringRavi Sachidanandam
Daniel Olivieri
Martina M Sykora
P2860cites workRecurrent insertion and duplication generate networks of transposable element sequences in the Drosophila melanogaster genomeQ21184150
A novel class of evolutionarily conserved genes defined by piwi are essential for stem cell self-renewalQ24595550
Unique germ-line organelle, nuage, functions to repress selfish genetic elements in Drosophila melanogasterQ24683818
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodQ25938999
piwi encodes a nucleoplasmic factor whose activity modulates the number and division rate of germline stem cellsQ28142394
The small RNA profile during Drosophila melanogaster developmentQ28201894
Notch-Delta signaling induces a transition from mitotic cell cycle to endocycle in Drosophila follicle cellsQ28208451
A genome-wide transgenic RNAi library for conditional gene inactivation in DrosophilaQ28236209
Biogenesis and germline functions of piRNAsQ28258430
P bodies: at the crossroads of post-transcriptional pathwaysQ28280058
A slicer-mediated mechanism for repeat-associated siRNA 5' end formation in DrosophilaQ28290099
Discrete small RNA-generating loci as master regulators of transposon activity in DrosophilaQ28292093
MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germlineQ28507423
Cytoplasmic compartmentalization of the fetal piRNA pathway in miceQ28507656
A piRNA pathway primed by individual transposons is linked to de novo DNA methylation in miceQ28511242
DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testesQ28513286
MOV10L1 is necessary for protection of spermatocytes against retrotransposons by Piwi-interacting RNAsQ28584864
Mouse MOV10L1 associates with Piwi proteins and is an essential component of the Piwi-interacting RNA (piRNA) pathwayQ28586946
Collapse of Germline piRNAs in the Absence of Argonaute3 Reveals Somatic piRNAs in FliesQ29028136
A distinct small RNA pathway silences selfish genetic elements in the germlineQ29614717
Small RNAs as guardians of the genomeQ29617221
Transposable elements and the epigenetic regulation of the genomeQ29617224
Functional involvement of Tudor and dPRMT5 in the piRNA processing pathway in Drosophila germlinesQ33553009
A broadly conserved pathway generates 3'UTR-directed primary piRNAsQ33614944
The Tetrahymena argonaute-binding protein Giw1p directs a mature argonaute-siRNA complex to the nucleus.Q33753063
Life cycle of an endogenous retrovirus, ZAM, in Drosophila melanogasterQ33787322
Proviral amplification of the Gypsy endogenous retrovirus of Drosophila melanogaster involves env-independent invasion of the female germlineQ33890892
Specialized piRNA pathways act in germline and somatic tissues of the Drosophila ovaryQ33901565
Double-stranded RNA-mediated silencing of genomic tandem repeats and transposable elements in the D. melanogaster germlineQ33953709
Female sterile mutations on the second chromosome of Drosophila melanogaster. II. Mutations blocking oogenesis or altering egg morphologyQ33958832
Flamenco, a gene controlling the gypsy retrovirus of Drosophila melanogasterQ33964490
Specific association of Piwi with rasiRNAs derived from retrotransposon and heterochromatic regions in the Drosophila genomeQ33998243
Conserved themes in small-RNA-mediated transposon controlQ34370338
A novel group of pumilio mutations affects the asymmetric division of germline stem cells in the Drosophila ovaryQ34430342
Regulation of I-transposon activity in Drosophila: evidence for cosuppression of nonhomologous transgenes and possible role of ancestral I-related pericentromeric elements.Q34616453
COM, a heterochromatic locus governing the control of independent endogenous retroviruses from Drosophila melanogaster.Q34617830
zucchini and squash encode two putative nucleases required for rasiRNA production in the Drosophila germlineQ34633591
Evidence for a piwi-dependent RNA silencing of the gypsy endogenous retrovirus by the Drosophila melanogaster flamenco geneQ34643946
The Drosophila RNA methyltransferase, DmHen1, modifies germline piRNAs and single-stranded siRNAs in RISC.Q34644431
Viral particles of the endogenous retrovirus ZAM from Drosophila melanogaster use a pre-existing endosome/exosome pathway for transfer to the oocyte.Q34881282
piRNAs mediate posttranscriptional retroelement silencing and localization to pi-bodies in the Drosophila germlineQ34995447
Identification of new X-chromosomal genes required for Drosophila oogenesis and novel roles for fs(1)Yb, brainiac and dunceQ35031425
Telomeric transgenes and trans-silencing in Drosophila.Q35118144
Establishment of stable cell lines of Drosophila germ-line stem cellsQ35133801
A novel repeat-associated small interfering RNA-mediated silencing pathway downregulates complementary sense gypsy transcripts in somatic cells of the Drosophila ovaryQ35635307
Pimet, the Drosophila homolog of HEN1, mediates 2'-O-methylation of Piwi- interacting RNAs at their 3' endsQ35855652
Cutoff and aubergine mutations result in retrotransposon upregulation and checkpoint activation in DrosophilaQ35867462
Repeat-associated siRNAs cause chromatin silencing of retrotransposons in the Drosophila melanogaster germlineQ36059543
Expression, characterization, and crystallization of a member of the novel phospholipase D family of phosphodiesterasesQ36280229
Integrin-dependent anchoring of a stem-cell nicheQ36487005
Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenesQ36580731
The Yb protein defines a novel organelle and regulates male germline stem cell self-renewal in Drosophila melanogasterQ37263977
Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family membersQ37287579
Arginine methylation of Piwi proteins catalysed by dPRMT5 is required for Ago3 and Aub stabilityQ37352427
Abundant primary piRNAs, endo-siRNAs, and microRNAs in a Drosophila ovary cell lineQ37395217
The Drosophila HP1 homolog Rhino is required for transposon silencing and piRNA production by dual-strand clusters.Q37406170
Gypsy transposition correlates with the production of a retroviral envelope-like protein under the tissue-specific control of the Drosophila flamenco gene.Q37636388
The large Maf factor Traffic Jam controls gonad morphogenesis in DrosophilaQ38348751
A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila.Q39790098
P-body formation is a consequence, not the cause, of RNA-mediated gene silencingQ42738042
Yb modulates the divisions of both germline and somatic stem cells through piwi- and hh-mediated mechanisms in the Drosophila ovaryQ47070008
Repression of retroelements in Drosophila germline via piRNA pathway by the Tudor domain protein TejasQ47070350
Somatic signaling mediated by fs(1)Yb is essential for germline stem cell maintenance during Drosophila oogenesisQ47070573
The Drosophila SDE3 homolog armitage is required for oskar mRNA silencing and embryonic axis specificationQ47071009
Arginine methyltransferase Capsuleen is essential for methylation of spliceosomal Sm proteins and germ cell formation in Drosophila.Q47071167
maelstrom is required for an early step in the establishment of Drosophila oocyte polarity: posterior localization of grk mRNA.Q47071323
Aubergine encodes a Drosophila polar granule component required for pole cell formation and related to eIF2C.Q47071373
Drosophila decapping protein 1, dDcp1, is a component of the oskar mRNP complex and directs its posterior localization in the oocyte.Q47072066
Homeless is required for RNA localization in Drosophila oogenesis and encodes a new member of the DE-H family of RNA-dependent ATPasesQ48069855
The RNA interference proteins and vasa locus are involved in the silencing of retrotransposons in the female germline of Drosophila melanogaster.Q50640910
RNAi-mediated inhibition of gene function in the follicle cell layer of the Drosophila ovary.Q52087242
tudor, a gene required for assembly of the germ plasm in Drosophila melanogaster.Q52264942
The molecular basis of P-M hybrid dysgenesis: the nature of induced mutations.Q52518771
The molecular basis of I-R hybrid dysgenesis in Drosophila melanogaster: identification, cloning, and properties of the I factorQ72388917
P433issue19
P407language of work or nameEnglishQ1860
P921main subjectDrosophilaQ312154
Piwi-interacting RNAQ2367421
Armitage Dmel_CG11513Q29809568
Female sterile (1) Yb Dmel_CG2706Q29809749
P-element induced wimpy testis Dmel_CG6122Q29814611
P304page(s)3301-3317
P577publication date2010-09-03
P1433published inThe EMBO JournalQ1278554
P1476titleAn in vivo RNAi assay identifies major genetic and cellular requirements for primary piRNA biogenesis in Drosophila
P478volume29

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