Cryo-EM structure of a human spliceosome activated for step 2 of splicing

scientific article published on 11 January 2017

Cryo-EM structure of a human spliceosome activated for step 2 of splicing is …
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scholarly articleQ13442814

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P6179Dimensions Publication ID1023854792
P356DOI10.1038/NATURE21079
P698PubMed publication ID28076346

P50authorReinhard Georg LührmannQ1317944
Cindy L WillQ63134178
Berthold KastnerQ63134443
Henning UrlaubQ28037184
Karl BertramQ41599411
P2093author name stringHolger Stark
Dmitry E Agafonov
Klaus Hartmuth
Olexandr Dybkov
Wen-Ti Liu
P2860cites workHuman step II splicing factor hSlu7 functions in restructuring the spliceosome between the catalytic steps of splicingQ22009171
Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centreQ24301865
The crystal structure of PPIL1 bound to cyclosporine A suggests a binding mode for a linear epitope of the SKIP proteinQ24305898
Solution structure of human peptidyl prolyl isomerase-like protein 1 and insights into its interaction with SKIPQ24314428
A human protein required for the second step of pre-mRNA splicing is functionally related to a yeast splicing factorQ24314559
Genetic and physical interactions between factors involved in both cell cycle progression and pre-mRNA splicing in Saccharomyces cerevisiaeQ24548138
The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctionsQ24595096
PHENIX: a comprehensive Python-based system for macromolecular structure solutionQ24654617
MolProbity: all-atom contacts and structure validation for proteins and nucleic acidsQ24684673
The architecture of the spliceosomal U4/U6.U5 tri-snRNP.Q27316447
Crystal structure of Prp8 reveals active site cavity of the spliceosomeQ27676016
Structure of a yeast spliceosome at 3.6-angstrom resolutionQ27701867
The 3.8 Å structure of the U4/U6.U5 tri-snRNP: Insights into spliceosome assembly and catalysisQ27703519
Molecular architecture of the human U4/U6.U5 tri-snRNPQ27704181
Structure of a yeast activated spliceosome at 3.5 Å resolutionQ27721209
Structure of a yeast catalytic step I spliceosome at 3.4 Å resolutionQ27721261
Cryo-EM structure of the spliceosome immediately after branchingQ27722247
Molecular architecture of the Saccharomyces cerevisiae activated spliceosomeQ27727737
SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modelingQ27860614
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nucleiQ27860728
Features and development of CootQ27861079
A U5 small nuclear ribonucleoprotein particle protein involved only in the second step of pre-mRNA splicing in Saccharomyces cerevisiaeQ27930011
Spliceosome activation by PRP2 ATPase prior to the first transesterification reaction of pre-mRNA splicingQ27931041
Prp45 affects Prp22 partition in spliceosomal complexes and splicing efficiency of non-consensus substrates.Q27932020
3D cryo-EM structure of an active step I spliceosome and localization of its catalytic coreQ41616762
GraFix: sample preparation for single-particle electron cryomicroscopyQ41623539
Structural bioinformatics of the human spliceosomal proteomeQ42286114
Lysine-specific chemical cross-linking of protein complexes and identification of cross-linking sites using LC-MS/MS and the xQuest/xProphet software pipelineQ42634194
Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicingQ42914749
Requirement of the RNA helicase-like protein PRP22 for release of messenger RNA from spliceosomesQ44165085
Suppression of multiple substrate mutations by spliceosomal prp8 alleles suggests functional correlations with ribosomal ambiguity mutantsQ47654036
Isolation of an active step I spliceosome and composition of its RNP coreQ50336090
Three-dimensional structure of C complex spliceosomes by electron microscopy.Q51021603
The HAT helix, a repetitive motif implicated in RNA processing.Q52561891
Structural basis of pre-mRNA splicing.Q53378420
The U5 and U6 small nuclear RNAs as active site components of the spliceosomeQ64383669
U5 snRNA interacts with exon sequences at 5′ and 3′ splice sitesQ67753779
Functional domains of the yeast splicing factor Prp22pQ73702880
Prp22, a DExH-box RNA helicase, plays two distinct roles in yeast pre-mRNA splicingQ27934705
Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed by dcFCCS.Q27935431
PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosomeQ27937219
Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal componentsQ27937542
Rearrangement of competing U2 RNA helices within the spliceosome promotes multiple steps in splicingQ27939473
Identification and characterization of Prp45p and Prp46p, essential pre-mRNA splicing factorsQ27939548
Molecular dissection of step 2 catalysis of yeast pre-mRNA splicing investigated in a purified systemQ27939740
The spliceosome: design principles of a dynamic RNP machineQ28131809
A large intrinsically disordered region in SKIP and its disorder-order transition induced by PPIL1 binding revealed by NMRQ28910448
RELION: implementation of a Bayesian approach to cryo-EM structure determinationQ29547673
Visualizing density maps with UCSF ChimeraQ29619793
Identification of cross-linked peptides from complex samples.Q30418939
RNA structure analysis of human spliceosomes reveals a compact 3D arrangement of snRNAs at the catalytic coreQ30556912
Characterization and functional ordering of Slu7p and Prp17p during the second step of pre-mRNA splicing in yeastQ33863083
Semiquantitative proteomic analysis of the human spliceosome via a novel two-dimensional gel electrophoresis methodQ34181884
Crystal structure of a eukaryotic group II intron lariatQ34339669
RNA catalyses nuclear pre-mRNA splicingQ34382976
DEAH-box ATPase Prp16 has dual roles in remodeling of the spliceosome in catalytic stepsQ34415414
Organization of core spliceosomal components U5 snRNA loop I and U4/U6 Di-snRNP within U4/U6.U5 Tri-snRNP as revealed by electron cryomicroscopyQ34575318
Evidence for a group II intron-like catalytic triplex in the spliceosomeQ34641230
A conformational rearrangement in the spliceosome sets the stage for Prp22-dependent mRNA releaseQ34788134
ModeRNA: a tool for comparative modeling of RNA 3D structureQ35017843
Allele-specific genetic interactions between Prp8 and RNA active site residues suggest a function for Prp8 at the catalytic core of the spliceosome.Q35202911
Functional interactions of Prp8 with both splice sites at the spliceosomal catalytic centerQ35203137
Insights into the mechanisms of splicing: more lessons from the ribosomeQ36276514
Movies of ice-embedded particles enhance resolution in electron cryo-microscopyQ36433748
Gel electrophoretic isolation of splicing complexes containing U1 small nuclear ribonucleoprotein particlesQ36783515
Spliceosomal DEAH-Box ATPases Remodel Pre-mRNA to Activate Alternative Splice SitesQ37165431
The mechanism of the second step of pre-mRNA splicingQ37947529
An essential splicing factor, SLU7, mediates 3' splice site choice in yeastQ38324793
Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 Å resolutionQ38584680
The mammalian homologue of Prp16p is overexpressed in a cell line tolerant to Leflunomide, a new immunoregulatory drug effective against rheumatoid arthritisQ41017180
Dissection of the factor requirements for spliceosome disassembly and the elucidation of its dissociation products using a purified splicing systemQ41553559
P433issue7641
P407language of work or nameEnglishQ1860
P921main subjectspliceosomeQ915868
cryogenic electron microscopyQ5190506
P304page(s)318-323
P577publication date2017-01-11
P1433published inNatureQ180445
P1476titleCryo-EM structure of a human spliceosome activated for step 2 of splicing
P478volume542

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