Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis

scientific article published on November 1997

Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis is …
instance of (P31):
scholarly articleQ13442814
review articleQ7318358

External links are
P356DOI10.1016/S0168-6445(97)00057-0
P698PubMed publication ID9451814

P50authorErko StackebrandtQ1355152
P2093author name stringGöbel UB
von Wintzingerode F
P433issue3
P921main subjectbiodiversityQ47041
microbial diversityQ124150969
P304page(s)213-229
P577publication date1997-11-01
P1433published inFEMS Microbiology ReviewsQ15762226
P1476titleDetermination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis
P478volume21

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Q33248703Estimation of microbial cover distributions at Mammoth Hot Springs using a multiple clone library resampling method
Q33716393Estimation of the relative abundance of different Bacteroides and Prevotella ribotypes in gut samples by restriction enzyme profiling of PCR-amplified 16S rRNA gene sequences.
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Q61799098Evaluation of Oral Cavity DNA Extraction Methods on Bacterial and Fungal Microbiota
Q39743064Evaluation of PCR amplification bias by terminal restriction fragment length polymorphism analysis of small-subunit rRNA and mcrA genes by using defined template mixtures of methanogenic pure cultures and soil DNA extracts
Q33988942Evaluation of PCR-generated chimeras, mutations, and heteroduplexes with 16S rRNA gene-based cloning
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Q34212021Evaluation of methods for the extraction and purification of DNA from the human microbiome
Q37191158Evaluation of single-nucleotide primer extension for detection and typing of phylogenetic markers used for investigation of microbial communities
Q33354792Evaluation of the bacterial diversity in the feces of cattle using 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP).
Q46857683Evaluation of the spoilage lactic acid bacteria in modified-atmosphere-packaged artisan-type cooked ham using culture-dependent and culture-independent approaches
Q42561281Evaluation of universal probes and primer sets for assessing total bacterial load in clinical samples: general implications and practical use in endodontic antimicrobial therapy
Q31063427Evolution of Bacterial Diversity during Enrichment of PCP-Degrading Activated Soils
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Q33242162Fecal bacterial diversity in a wild gorilla
Q33384651Flavobacterial community structure in a hardwater rivulet and adjacent forest soil, Harz Mountain, Germany
Q30924732Formation of pseudo-terminal restriction fragments, a PCR-related bias affecting terminal restriction fragment length polymorphism analysis of microbial community structure
Q42090155Frequent in vitro recombination in internal transcribed spacers 1 and 2 during genotyping of Pneumocystis jirovecii
Q50103995Functional Transcripts Indicate Phylogenetically Diverse Active Ammonia-Scavenging Microbiota in Sympatric Sponges.
Q30835828Fungal rDNA signatures in coronary atherosclerotic plaques
Q33325849Genetic diversity of eukaryotic microorganisms in Lake Taihu, a large shallow subtropical lake in china
Q33224564Genetic diversity of small eukaryotes in lakes differing by their trophic status
Q33277339Genotypic microbial community profiling: a critical technical review
Q34098084Geobacteraceae community composition is related to hydrochemistry and biodegradation in an iron-reducing aquifer polluted by a neighboring landfill
Q40897177Geographic and environmental sources of variation in lake bacterial community composition
Q34244003Gut microbiome of the critically endangered New Zealand parrot, the kakapo (Strigops habroptilus).
Q30833779Heteroduplexes in mixed-template amplifications: formation, consequence and elimination by 'reconditioning PCR'
Q37550442High diversity of airborne fungi in the hospital environment as revealed by meta-sequencing-based microbiome analysis
Q37293115High diversity of fungi in air particulate matter
Q33283355High local and global diversity of Flavobacteria in marine plankton
Q40311269High-sensitivity stable-isotope probing by a quantitative terminal restriction fragment length polymorphism protocol
Q35272069Highly sensitive direct detection and quantification of Burkholderia pseudomallei bacteria in environmental soil samples by using real-time PCR.
Q46922082Hormone therapy alters the composition of the vaginal microflora in ovariectomized rats
Q39101245Host specificity in the Richelia-diatom symbiosis revealed by hetR gene sequence analysis
Q33336207Human methanogen diversity and incidence in healthy and diseased colonic groups using mcrA gene analysis.
Q33850506Hydrogen and bioenergetics in the Yellowstone geothermal ecosystem
Q34579414Identification and quantification of methanogenic Archaea in adult chicken ceca
Q33965894Identification by 16S rRNA gene analyses of a potential novel mycobacterial species as an etiological agent of canine leproid granuloma syndrome
Q33986557Identification of and spatio-temporal differences between microbial assemblages from two neighboring sulfurous lakes: comparison by microscopy and denaturing gradient gel electrophoresis.
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Q42727445Investigation of the microbial ecology of intertidal hot springs by using diversity analysis of 16S rRNA and chitinase genes
Q27500368Iron-Based Microbial Ecosystem on and Below the Seafloor: A Case Study of Hydrothermal Fields of the Southern Mariana Trough
Q37718495Isotopic signatures of N2O produced by ammonia-oxidizing archaea from soils
Q33900718Lack of phylogeographic structure in the freshwater cyanobacterium Microcystis aeruginosa suggests global dispersal
Q51242950Lactic acid bacteria associated with wine grapes from several Australian vineyards
Q28395398Lessons learned and unlearned in periodontal microbiology
Q46244285Linking bacterial community structure to advection and environmental impact along a coast-fjord gradient of the Sognefjord, western Norway
Q30392846Linking phylogeny to abundant ribotypes of community fingerprints: an exercise on the phylotypic responses to plant species, fertilisation and Lolium perenne ingression
Q28661752Long-range dispersal and high-latitude environments influence the population structure of a "stress-tolerant" dinoflagellate endosymbiont
Q33221273Low intraspecific diversity in a polynucleobacter subcluster population numerically dominating bacterioplankton of a freshwater pond
Q48082659Low nitrification rates in acid Scots pine forest soils are due to pH-related factors
Q43023823Lysis efficiency of standard DNA extraction methods for Halococcus spp. in an organic rich environment.
Q33233889Measurement of Prochlorococcus ecotypes using real-time polymerase chain reaction reveals different abundances of genotypes with similar light physiologies
Q42845694Measuring species richness based on microbial community fingerprints: the emperor has no clothes.
Q33186494Mechanisms underlying the impact of humic acids on DNA quantification by SYBR Green I and consequences for the analysis of soils and aquatic sediments
Q34734523Mercury resistance determinants related to Tn21, Tn1696, and Tn5053 in enterobacteria from the preantibiotic era.
Q33302198Metabolically active microbial communities of yellow and grey colonizations on the walls of Altamira Cave, Spain.
Q28710421Metagenomic analysis of a southern maritime antarctic soil
Q34179603Metagenomic analysis of a stable trichloroethene-degrading microbial community.
Q47096204Metagenomic investigation of gastrointestinal microbiome in cattle.
Q31151981Metagenomic profiling: microarray analysis of an environmental genomic library
Q33849162Method for preparing DNA from feces in guanidine thiocyanate solution affects 16S rRNA-based profiling of human microbiota diversity.
Q35135101Methodologies for the characterization of microbes in industrial environments: a review
Q34609643Methods for estimating gene frequencies and detecting selection in bacterial populations.
Q28660449Methyl coenzyme M reductase (mcrA) gene abundance correlates with activity measurements of methanogenic H₂ /CO₂ -enriched anaerobic biomass
Q33253371Microarray applications in microbial ecology research
Q49460345Microbial Communities and Diversities in Mudflat Sediments Analyzed Using a Modified Metatranscriptomic Method
Q38346746Microbial DNA extraction from intestinal biopsies is improved by avoiding mechanical cell disruption.
Q44016567Microbial and mineralogical characterizations of soils collected from the deep biosphere of the former Homestake gold mine, South Dakota
Q80176984Microbial communities and immigration in volcanic environments of Canary Islands (Spain)
Q33985423Microbial communities associated with anaerobic benzene degradation in a petroleum-contaminated aquifer
Q34998839Microbial communities associated with geological horizons in coastal subseafloor sediments from the sea of okhotsk.
Q34057632Microbial communities from methane hydrate-bearing deep marine sediments in a forearc basin
Q89931322Microbial community analysis using high-throughput sequencing technology: a beginner's guide for microbiologists
Q24550501Microbial community dynamics during production of the Mexican fermented maize dough pozol
Q33746279Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis
Q24651959Microbial community gene expression in ocean surface waters
Q34017154Microbial community profiling for human microbiome projects: Tools, techniques, and challenges
Q57231421Microbial community related to volatile organic compound (VOC) emission in household biowaste
Q33194643Microbial community structure in midgut and hindgut of the humus-feeding larva of Pachnoda ephippiata (Coleoptera: Scarabaeidae).
Q31158398Microbial diversity of benthic mats along a tidal desiccation gradient
Q48123637Microbial diversity of wild bird feathers revealed through culture-based and culture-independent techniques
Q57031055Microbial enrichment and gene functional categories revealed on the walls of a spent fuel pool of a nuclear power plant
Q38578772Microbial phylogenetic profiling with the Pacific Biosciences sequencing platform
Q33363136Microbial prevalence, diversity and abundance in amniotic fluid during preterm labor: a molecular and culture-based investigation
Q43026355Microbiology and geochemistry of great boiling and mud hot springs in the United States Great Basin
Q34855203Microbiology of diabetic foot infections: from Louis Pasteur to 'crime scene investigation'.
Q37466193Microbiota of the seminal fluid from healthy and infertile men.
Q43750279Microcosm enrichment of 1,3-dichloropropene-degrading soil microbial communities in a compost-amended soil.
Q53144155Microenvironments and microbial community structure in sediments.
Q35639427Microfluidic approaches to malaria detection
Q34052518Microheterogeneity in 16S ribosomal DNA-defined bacterial populations from a stratified planktonic environment is related to temporal changes and to ecological adaptations.
Q30988204Microorganisms with a taste for vanilla: microbial ecology of traditional Indonesian vanilla curing
Q33353282Microscopic and molecular studies of the diversity of free-living protozoa in meat-cutting plants
Q31029138Microsite-dependent changes in methanogenic populations in a boreal oligotrophic fen.
Q42706625Modified RNA-seq method for microbial community and diversity analysis using rRNA in different types of environmental samples
Q60238637Molecular Analysis of Microbiota Along the Digestive Tract of Juvenile Atlantic Salmon (Salmo salar L.)
Q35956116Molecular Detection of 10 of the Most Unwanted Alien Forest Pathogens in Canada Using Real-Time PCR.
Q48368147Molecular analyses of methyl-coenzyme M reductase alpha-subunit (mcrA) genes in rice field soil and enrichment cultures reveal the methanogenic phenotype of a novel archaeal lineage
Q30658788Molecular analysis of bacterial community structure and diversity in unimproved and improved upland grass pastures
Q37572032Molecular analysis of geographic patterns of eukaryotic diversity in Antarctic soils
Q33301872Molecular analysis of the diversity of sulfate-reducing and sulfur-oxidizing prokaryotes in the environment, using aprA as functional marker gene
Q33849401Molecular characterization of activated sludge from a seawater-processing wastewater treatment plant
Q39488244Molecular characterization of bacterial populations in petroleum-contaminated groundwater discharged from underground crude oil storage cavities
Q31423320Molecular characterization of functional and phylogenetic genes from natural populations of methanotrophs in lake sediments.
Q33719863Molecular characterization of resistance-nodulation-division transporters from solvent- and drug-resistant bacteria in petroleum-contaminated soil
Q48099708Molecular characterization of the bacteria adherent to human colorectal mucosa
Q33986835Molecular diversity within Lactobacillus helveticus as revealed by genotypic characterization
Q31105614Molecular evidence for a uniform microbial community in sponges from different oceans
Q34316194Molecular evidence for association of chlamydiales bacteria with epitheliocystis in leafy seadragon (Phycodurus eques), silver perch (Bidyanus bidyanus), and barramundi (Lates calcarifer).
Q34345435Molecular evidence that plastids in the toxin-producing dinoflagellate genus Dinophysis originate from the free-living cryptophyte Teleaulax amphioxeia
Q24685677Molecular identification of ectomycorrhizal mycelium in soil horizons
Q33966566Molecular phylogenetic evidence for noninvasive zoonotic transmission of Staphylococcus intermedius from a canine pet to a human
Q28393306Molecular profiling of fungal communities in moisture damaged buildings before and after remediation--a comparison of culture-dependent and culture-independent methods
Q24680349Molecular profiling of the Clostridium leptum subgroup in human fecal microflora by PCR-denaturing gradient gel electrophoresis and clone library analysis
Q33319808Monitoring exogenous and indigenous bacteria by PCR-DGGE technology during the process of microbial enhanced oil recovery
Q37433707Multi-template polymerase chain reaction
Q33844534Multiple DNA extractions coupled with stable-isotope probing of anthracene-degrading bacteria in contaminated soil
Q35946772Multivariate analysis of complex DNA sequence electropherograms for high-throughput quantitative analysis of mixed microbial populations
Q34100156Natural communities of novel archaea and bacteria with a string-of-pearls-like morphology: molecular analysis of the bacterial partners
Q36483454Natural gas and temperature structured a microbial community response to the Deepwater Horizon oil spill.
Q33507276New insights into the diversity of marine picoeukaryotes
Q41031523New real-time quantitative PCR procedure for quantification of bifidobacteria in human fecal samples
Q33806208Next-generation sequencing in the analysis of human microbiota: essential considerations for clinical application.
Q33895057Nitrogenase gene amplicons from global marine surface waters are dominated by genes of non-cyanobacteria
Q39805317Non-culture-based analysis of bacterial populations from patients with chronic rhinosinusitis
Q33282396Novel and unexpected prokaryotic diversity in water and sediments of the alkaline, hypersaline lakes of the Wadi An Natrun, Egypt
Q33310394Novel archaea and bacteria dominate stable microbial communities in North America's Largest Hot Spring
Q33988811Novel bacterial lineages at the (sub)division level as detected by signature nucleotide-targeted recovery of 16S rRNA genes from bulk soil and rice roots of flooded rice microcosms
Q33218777Novel predominant archaeal and bacterial groups revealed by molecular analysis of an anaerobic sludge digester
Q30804939Numerical dominance and phylotype diversity of marine Rhodobacter species during early colonization of submerged surfaces in coastal marine waters as determined by 16S ribosomal DNA sequence analysis and fluorescence in situ hybridization
Q89440872Nutrient Enrichment Alters Salt Marsh Fungal Communities and Promotes Putative Fungal Denitrifiers
Q39648709Oligonucleotide microarray for 16S rRNA gene-based detection of all recognized lineages of sulfate-reducing prokaryotes in the environment
Q79370772On the use of denaturing gradient gel electrophoresis approach for bacterial identification in endodontic infections
Q67237609Ontogenetic Development of the Gastrointestinal Microbiota in the Marine Herbivorous Fish Kyphosus sydneyanus
Q83973907Optimization of the DGGE band identification method
Q39884765PCR amplification of repetitive DNA: a limitation to genome editing technologies and many other applications.
Q33963322PCR amplification-independent methods for detection of microbial communities by the high-density microarray PhyloChip
Q39488359PCR bias in ecological analysis: a case study for quantitative Taq nuclease assays in analyses of microbial communities
Q55038449PCR detection and 16S rRNA sequence-based phylogeny of a novel Propionibacterium acidipropionici applicable for enhanced fermentation of high moisture corn.
Q51643701PCR-denaturing gradient gel electrophoresis of complex microbial communities: a two-step approach to address the effect of gel-to-gel variation and allow valid comparisons across a large dataset.
Q33228737PCR-induced sequence artifacts and bias: insights from comparison of two 16S rRNA clone libraries constructed from the same sample.
Q30835560Peptide nucleic acid-mediated PCR clamping as a useful supplement in the determination of microbial diversity
Q34738337Persistence of free-living protozoan communities across rearing cycles in commercial poultry houses
Q61811203Phenotyping and outcomes of hospitalized COPD patients using rapid molecular diagnostics on sputum samples
Q55842369Phylogenetic analyses of Caulerpa taxifolia (Chlorophyta) and of its associated bacterial microflora provide clues to the origin of the Mediterranean introduction
Q33543844Phylogenetic analysis of 16S rRNA gene sequences reveals distal gut bacterial diversity in wild wolves (Canis lupus).
Q33503765Phylogenetic analysis of 16S rRNA gene sequences reveals rumen bacterial diversity in Yaks (Bos grunniens).
Q33984115Phylogenetic analysis of an anaerobic, trichlorobenzene-transforming microbial consortium
Q30913832Phylogenetic analysis of bacterial communities in mesophilic and thermophilic bioreactors treating pharmaceutical wastewater
Q42629690Phylogenetic analysis of intestinal bacteria in the Chinese mitten crab (Eriocheir sinensis).
Q33985477Phylogenetic analysis of particle-attached and free-living bacterial communities in the Columbia river, its estuary, and the adjacent coastal ocean.
Q33204518Phylogenetic analysis of protozoa in the rumen contents of cow based on the 18S rDNA sequences
Q30746512Phylogenetic analysis of the microbial populations in the wild herbivore gastrointestinal tract: insights into an unexplored niche
Q30804954Phylogenetic composition of bacterioplankton assemblages from the Arctic Ocean
Q37371877Phylogenetic diversity and spatial distribution of the microbial community associated with the Caribbean deep-water sponge Polymastia cf. corticata by 16S rRNA, aprA, and amoA gene analysis
Q41829281Phylogenetic diversity of archaea and bacteria in the anoxic zone of a meromictic lake (Lake Pavin, France).
Q33201411Phylogenetic diversity of drinking water bacteria in a distribution system simulator
Q33280754Phylogenetic diversity of gram-positive bacteria cultured from marine sediments
Q33293695Phylogenetic diversity of the archaeal community in a continental high-temperature, water-flooded petroleum reservoir.
Q33193744Phylogenetic diversity, abundance, and axial distribution of bacteria in the intestinal tract of two soil-feeding termites (Cubitermes spp.).
Q33988306Phylogeny of all recognized species of ammonia oxidizers based on comparative 16S rRNA and amoA sequence analysis: implications for molecular diversity surveys
Q24616767Plankton microorganisms coinciding with two consecutive mass fish kills in a newly reconstructed lake
Q39484235Polyphasic study of the spatial distribution of microorganisms in Mexican pozol, a fermented maize dough, demonstrates the need for cultivation-independent methods to investigate traditional fermentations
Q30855884Polyphosphate kinase from activated sludge performing enhanced biological phosphorus removal
Q43368566Population dynamics of a single-stage sulfidogenic bioreactor treating synthetic zinc-containing waste streams
Q33984765Populations of select cultured and uncultured bacteria in the rumen of sheep and the effect of diets and ruminal fractions
Q36945411Potential applications of next generation DNA sequencing of 16S rRNA gene amplicons in microbial water quality monitoring.
Q34011041Prevalence and diversity of microbes in the amniotic fluid, the fetal inflammatory response, and pregnancy outcome in women with preterm pre-labor rupture of membranes
Q33960902Prevalence of corynebacterial 16S rRNA sequences in patients with bacterial and "nonbacterial" prostatitis.
Q33708503Prevalence of the Chloroflexi-related SAR202 bacterioplankton cluster throughout the mesopelagic zone and deep ocean
Q46465335Profile of Citrobacter freundii ST2, a Multi-acyl-homoserine Lactone Producer Associated with Marine Dinoflagellates
Q24675394Prokaryote diversity and taxonomy: current status and future challenges
Q31081616Prokaryotic genetic diversity throughout the salinity gradient of a coastal solar saltern.
Q33406826Prokaryotic genomes and diversity in surface ocean waters: interrogating the global ocean sampling metagenome
Q34986471Prokaryotic metabolic activity and community structure in Antarctic continental shelf sediments
Q42013636Protist genetic diversity in the acidic hydrothermal environments of Lassen Volcanic National Park, USA.
Q99552872Putative degraders of low-density polyethylene-derived compounds are ubiquitous members of plastic-associated bacterial communities in the marine environment
Q34780290Quantification of intestinal bacterial populations by real-time PCR with a universal primer set and minor groove binder probes: a global approach to the enteric flora
Q43064023Quantification of the detrimental effect of a single primer-template mismatch by real-time PCR using the 16S rRNA gene as an example.
Q33984188Quantifying microbial diversity: morphotypes, 16S rRNA genes, and carotenoids of oxygenic phototrophs in microbial mats
Q92659034Quantitative PCR provides a simple and accessible method for quantitative microbiota profiling
Q35720077Quantitative analysis of diverse Lactobacillus species present in advanced dental caries
Q33987932Quantitative analysis of small-subunit rRNA genes in mixed microbial populations via 5'-nuclease assays
Q33406834Quantitative community fingerprinting methods for estimating the abundance of operational taxonomic units in natural microbial communities
Q31004958Quantitative comparisons of 16S rRNA gene sequence libraries from environmental samples
Q40515113Quantitative detection of periodontal pathogens using real-time polymerase chain reaction with TaqMan probes
Q42085577Quantitative molecular assay for fingerprinting microbial communities of wastewater and estrogen-degrading consortia
Q33372289Quantitative rRNA-targeted solution-based hybridization assay using peptide nucleic acid molecular beacons
Q34998687Quantitative real-time PCR for determination of microcystin synthetase e copy numbers for microcystis and anabaena in lakes.
Q39640742Quantitative tracing, by Taq nuclease assays, of a synechococcus ecotype in a highly diversified natural population
Q33233893Quantitative, longitudinal profiling of the primate fecal microbiota reveals idiosyncratic, dynamic communities
Q64234315Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION™ nanopore sequencer
Q25257215Rapid quantitative profiling of complex microbial populations
Q21146690Reagent and laboratory contamination can critically impact sequence-based microbiome analyses
Q34055663Real-time PCR quantification of rbcL (ribulose-1,5-bisphosphate carboxylase/oxygenase) mRNA in diatoms and pelagophytes
Q90396533Recent Advancements in Intestinal Microbiota Analyses: A Review for Non-Microbiologists
Q33766262Reducing Viability Bias in Analysis of Gut Microbiota in Preterm Infants at Risk of NEC and Sepsis
Q37702240Reevaluation and reduction of a PCR bias caused by reannealing of templates.
Q33214666Relationship between bacterioplankton richness, respiration, and production in the Southern North Sea.
Q31981307Relationships between microbial community structure and hydrochemistry in a landfill leachate-polluted aquifer.
Q46392957Relationships between sediment microbial communities and pollutants in two California salt marshes
Q42001432Relative and Absolute Quantitative Real-Time PCR-Based Quantifications of hcnC and phlD Gene Transcripts in Natural Soil Spiked with Pseudomonas sp. Strain LBUM300
Q43314056Removal of carbonaceous and nitrogenous pollutants from a synthetic wastewater using a membrane-coupled bioreactor
Q51715170Residual polymerase activity-induced bias in terminal restriction fragment length polymorphism analysis.
Q45719002Resolving Species Level Changes in a Representative Soil Bacterial Community Using Microfluidic Quantitative PCR.
Q40832943Responses of active bacterial and fungal communities in soils under winter wheat to different fertilizer and pesticide regimens
Q43712374Retrieval of nearly complete 16S rRNA gene sequences from environmental DNA following 16S rRNA-based community fingerprinting
Q33185022Robust hydrocarbon degradation and dynamics of bacterial communities during nutrient-enhanced oil spill bioremediation
Q34165899SAR11 clade dominates ocean surface bacterioplankton communities.
Q34141458Sample richness and genetic diversity as drivers of chimera formation in nSSU metagenetic analyses
Q45934630Screening and identification of new isolate: thermostable Escherichia coli with novel thermoalkalotolerant cellulases.
Q30399744Search and discovery strategies for biotechnology: the paradigm shift
Q31062173Seasonal dynamics of particle-associated and free-living marine Proteobacteria in a salt marsh tidal creek as determined using fluorescence in situ hybridization
Q33730166Second-generation environmental sequencing unmasks marine metazoan biodiversity
Q34993355Secretory antibodies do not affect the composition of the bacterial microbiota in the terminal ileum of 10-week-old mice
Q33633022Sequence diversity and novelty of natural assemblages of picoeukaryotes from the Indian Ocean
Q54682890Serial analysis of V6 ribosomal sequence tags (SARST-V6): a method for efficient, high-throughput analysis of microbial community composition.
Q30851650Shifts in rhizoplane communities of aquatic plants after cadmium exposure
Q39508528Short chain N-acyl homoserine lactone production in tropical marine Vibrio sinaloensis strain T47.
Q36018114Simple absolute quantification method correcting for quantitative PCR efficiency variations for microbial community samples
Q42071042Simple and safe method for simultaneous isolation of microbial RNA and DNA from problematic populations
Q36232698Simultaneous Extraction of Viral and Bacterial Nucleic Acids for Molecular Diagnostic Applications
Q33986867Simultaneous detection and differentiation of Escherichia coli populations from environmental freshwaters by means of sequence variations in a fragment of the beta-D-glucuronidase gene
Q39638988Simultaneous extraction from bacterioplankton of total RNA and DNA suitable for quantitative structure and function analyses.
Q33990411Simultaneous recovery of RNA and DNA from soils and sediments
Q39789044Single-cell protein profiling of wastewater enterobacterial communities predicts disinfection efficiency
Q81575521Soil bacterial and fungal community structure across a range of unimproved and semi-improved upland grasslands
Q34342238Soil eukaryotic microorganism succession as affected by continuous cropping of peanut--pathogenic and beneficial fungi were selected
Q31077093Soil fungal communities underneath willow canopies on a primary successional glacier forefront: rDNA sequence results can be affected by primer selection and chimeric data
Q33884297Spatial Heterogeneity of Bacteria: Evidence from Hot Composts by Culture-independent Analysis
Q30790282Spatial and resource factors influencing high microbial diversity in soil
Q51697102Spatial and temporal changes in microbial community structure associated with recharge-influenced chemical gradients in a contaminated aquifer.
Q39527602Spatial heterogeneity of bacterial populations along an environmental gradient at a shallow submarine hydrothermal vent near Milos Island (Greece).
Q33310417Spatial heterogeneity of bacterial populations in monomictic Lake Estanya (Huesca, Spain).
Q33330731Species divergence and the measurement of microbial diversity
Q34986750Specific Detection of Dehalococcoides Species by Fluorescence In Situ Hybridization with 16S rRNA-Targeted Oligonucleotide Probes
Q30477192Specific bacterial, archaeal, and eukaryotic communities in tidal-flat sediments along a vertical profile of several meters
Q35663420Specific response of a novel and abundant Lactobacillus amylovorus-like phylotype to dietary prebiotics in the guts of weaning piglets
Q90268864Spiking a Silty-Sand Reference Soil with Bacterial DNA: Limits and Pitfalls in the Discrimination of Live and Dead Cells When Applying Ethidium Monoazide (EMA) Treatment
Q31081627Stratification and seasonal stability of diverse bacterial communities in a Pinus merkusii (pine) forest soil in central Java, Indonesia.
Q37318874Stratification of activity and bacterial community structure in biofilms grown on membranes transferring oxygen
Q33221274Structure and topology of microbial communities in the major gut compartments of Melolontha melolontha larvae (Coleoptera: Scarabaeidae).
Q30993845Study of genetic diversity of eukaryotic picoplankton in different oceanic regions by small-subunit rRNA gene cloning and sequencing
Q33296990Studying seabird diet through genetic analysis of faeces: a case study on macaroni penguins (Eudyptes chrysolophus)
Q52544172Succession of microbial communities during a biostimulation process as evaluated by DGGE and clone library analyses.
Q54563039Taxon-specific PCR primers to detect two inconspicuous arbuscular mycorrhizal fungi from temperate agricultural grassland.
Q37997281Techniques used to characterize the gut microbiota: a guide for the clinician.
Q33718086Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria.
Q41986854Template Preparation Affects 16S rRNA High-Throughput Sequencing Analysis of Phyllosphere Microbial Communities
Q37111227Temporal Shifts in Microbial Communities in Nonpregnant African-American Women with and without Bacterial Vaginosis
Q89882397Tendentious effects of automated and manual metagenomic DNA purification protocols on broiler gut microbiome taxonomic profiling
Q39486765Terminal restriction fragment length polymorphism analysis program, a web-based research tool for microbial community analysis
Q34333032The Microbial Ferrous Wheel in a Neutral pH Groundwater Seep
Q26765740The New Era of Treatment for Obesity and Metabolic Disorders: Evidence and Expectations for Gut Microbiome Transplantation
Q26774698The Role of Ciliate Protozoa in the Rumen
Q33748729The bias associated with amplicon sequencing does not affect the quantitative assessment of bacterial community dynamics
Q43971505The diversity and function of soil microbial communities exposed to different disturbances.
Q37111236The human vaginal bacterial biota and bacterial vaginosis.
Q53280745The impact of storage buffer, DNA extraction method, and polymerase on microbial analysis.
Q51159195The importance of sample archiving in microbial ecology
Q37055203The isotope array, a new tool that employs substrate-mediated labeling of rRNA for determination of microbial community structure and function
Q35012395The microbiome of the upper airways: focus on chronic rhinosinusitis
Q29615054The pervasive effects of an antibiotic on the human gut microbiota, as revealed by deep 16S rRNA sequencing
Q33231939The reductive dechlorination of 2,3,4,5-tetrachlorobiphenyl in three different sediment cultures: evidence for the involvement of phylogenetically similar Dehalococcoides-like bacterial populations
Q39267205The relationship between microbial community structure and functional stability, tested experimentally in an upland pasture soil.
Q24811602The role of syntrophic associations in sustaining anaerobic mineralization of chlorinated organic compounds
Q33283875The use of molecular techniques based on ribosomal RNA and DNA for rumen microbial ecosystem studies: a review
Q42685611The use of unilateral PCR to identify prominent heteroduplexes formed during PCR of the mouse microsatellite locus D17Mit23.
Q33984043Thermal gradient gel electrophoresis analysis of bioprotection from pollutant shocks in the activated sludge microbial community.
Q34031643Tidying up international nucleotide sequence databases: ecological, geographical and sequence quality annotation of its sequences of mycorrhizal fungi
Q33232442Toxic Microcystis is widespread in Lake Erie: PCR detection of toxin genes and molecular characterization of associated cyanobacterial communities
Q36131283Uncovering Trophic Interactions in Arthropod Predators through DNA Shotgun-Sequencing of Gut Contents.
Q34098048Unexpectedly high bacterial diversity in arctic tundra relative to boreal forest soils, revealed by serial analysis of ribosomal sequence tags
Q29547435UniFrac: a new phylogenetic method for comparing microbial communities
Q33396551Universal ligation-detection-reaction microarray applied for compost microbes
Q31105626Unveiling the organisms behind novel eukaryotic ribosomal DNA sequences from the ocean
Q34364363Unveiling trophic functions of uncultured protist taxa by incubation experiments in the brackish Baltic Sea
Q24535027Use of 16S rRNA gene terminal restriction fragment analysis to assess the impact of solids retention time on the bacterial diversity of activated sludge
Q34101072Use of 16S ribosomal DNA for delineation of marine bacterioplankton species
Q34565267Use of molecular techniques to study microbial diversity in the skin: chronic wounds reevaluated.
Q33776333Vaginal and Uterine Bacterial Communities in Postpartum Lactating Cows.
Q31029577Variation in growth rate in a natural assemblage of unicellular green soil algae
Q39493665Variation of microbial communities in soil, rhizosphere, and rhizoplane in response to crop species, soil type, and crop development
Q43024487Vertical distribution of bacterial and archaeal communities along discrete layers of a deep-sea cold sediment sample at the East Pacific Rise (approximately 13 degrees N).
Q33283354Vertical distribution of picoeukaryotic diversity in the Sargasso Sea.
Q35095064Vertical physico-chemical gradients with distinct microbial communities in the hypersaline and heliothermal Lake Ursu (Sovata, Romania).
Q33330100Wide genetic diversity of picoplanktonic green algae (Chloroplastida) in the Mediterranean Sea uncovered by a phylum-biased PCR approach
Q40467883Within- and between-lake variability in the composition of bacterioplankton communities: investigations using multiple spatial scales.
Q33296434Yeast community structures and dynamics in healthy and Botrytis-affected grape must fermentations
Q37597340characterization of bacterial community diversity in cystic fibrosis lung infections by use of 16s ribosomal DNA terminal restriction fragment length polymorphism profiling
Q93381110pahE, a Functional Marker Gene for Polycyclic Aromatic Hydrocarbon-Degrading Bacteria
Q34009966rRNASelector: a computer program for selecting ribosomal RNA encoding sequences from metagenomic and metatranscriptomic shotgun libraries
Q33987399rpoB-based microbial community analysis avoids limitations inherent in 16S rRNA gene intraspecies heterogeneity.
Q34314931rrndb: the Ribosomal RNA Operon Copy Number Database

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