A Fyn biosensor reveals pulsatile, spatially localized kinase activity and signaling crosstalk in live mammalian cells

scientific article published on 04 February 2020

A Fyn biosensor reveals pulsatile, spatially localized kinase activity and signaling crosstalk in live mammalian cells is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.7554/ELIFE.50571
P932PMC publication ID7000222
P698PubMed publication ID32017701

P50authorAkash GulyaniQ73298751
Balaji M RaoQ88768826
Ranabir DasQ89293560
Ananya MukherjeeQ89526866
Randhir SinghQ89526868
Sreeram UdayanQ89526870
Sayan BiswasQ89526872
P2093author name stringGeen George
Pothula Purushotham Reddy
Saumya Manmadhan
Shilpa Kumar
P2860cites workProfound block in thymocyte development in mice lacking p56lckQ24298882
A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasisQ24307454
SU6656, a selective src family kinase inhibitor, used to probe growth factor signalingQ24551043
Determination of solution structures of proteins up to 40 kDa using CS-Rosetta with sparse NMR data from deuterated samplesQ24633467
Latent bone metastasis in breast cancer tied to Src-dependent survival signalsQ24648828
Neuroblastoma tyrosine kinase signaling networks involve FYN and LYN in endosomes and lipid raftsQ27319837
The role of the Src homology 3-Src homology 2 interface in the regulation of Src kinasesQ27630908
Active site profiling reveals coupling between domains in SRC-family kinasesQ27675080
Crystal structure of the SH3 domain in human Fyn; comparison of the three-dimensional structures of SH3 domains in tyrosine kinases and spectrinQ27729913
Rapid and efficient site-specific mutagenesis without phenotypic selectionQ27860628
Cell migration: integrating signals from front to backQ27860670
NMRPipe: a multidimensional spectral processing system based on UNIX pipesQ27860859
Fyn is induced by Ras/PI3K/Akt signaling and is required for enhanced invasion/migrationQ28235184
Cortactin-Src kinase signaling pathway is involved in N-syndecan-dependent neurite outgrowthQ28268436
Absence of Fyn and Src causes a reeler-like phenotypeQ28272263
A requirement for caveolin-1 and associated kinase Fyn in integrin signaling and anchorage-dependent cell growthQ28282803
FRET and colocalization analyzer--a method to validate measurements of sensitized emission FRET acquired by confocal microscopy and available as an ImageJ Plug-inQ28364759
Visualization of Src and FAK Activity during the Differentiation Process from HMSCs to OsteoblastsQ28482181
Integrins direct Src family kinases to regulate distinct phases of oligodendrocyte developmentQ28582937
Live Cell Imaging of Src/FAK Signaling by FRET.Q42870258
CNS integrins switch growth factor signalling to promote target-dependent survivalQ44179357
Avidity‐mediated virus separation using a hyperthermophilic affinity ligandQ44866390
Genetically encoded Förster resonance energy transfer sensors for the conformation of the Src family kinase Lck.Q44881978
Specification of the direction of adhesive signaling by the integrin beta cytoplasmic domainQ46171254
Optimization of codon pair use within the (GGGGS)3 linker sequence results in enhanced protein expressionQ46192483
Fyn kinase induces synaptic and cognitive impairments in a transgenic mouse model of Alzheimer's disease.Q46463850
Disease-related modifications in tau affect the interaction between Fyn and Tau.Q46663739
Expression of Fyn kinase modulates EMT in oral cancer cellsQ46678609
Stimulation of the protein tyrosine kinase c-Yes but not c-Src by neurotrophins in human brain-metastatic melanoma cellsQ47775802
Excitable Signal Transduction Networks in Directed Cell Migration.Q47813317
Direct measurement of oscillatory RhoA activity in embryonic cortical neurons stimulated with the axon guidance cue netrin-1 using fluorescence resonance energy transferQ48962837
A biosensor for MAPK-dependent Lin28 signalingQ54121343
T cell activation results in conformational changes in the Src family kinase Lck to induce its activationQ54448667
MOBP levels are regulated by Fyn kinase and affect the morphological differentiation of oligodendrocytes.Q55031751
Cytoplasmic sequestration of the RhoA effector mDiaphanous1 by Prohibitin2 promotes muscle differentiation.Q64934091
Characterization of pp60c-src Tyrosine Kinase Activities Using a Continuous Assay: Autoactivation of the Enzyme Is an Intermolecular Autophosphorylation ProcessQ71786430
A method for assessment and processing of biomedical signals containing trend and periodic componentsQ74657926
Detection of Protein-Protein Interactions Using the GST Fusion Protein Pulldown TechniqueQ83833702
Novel FRET-Based Src Biosensor Reveals Mechanisms of Src Activation and Its Dynamics in Focal AdhesionsQ90567652
Sensitive FRET Biosensor Reveals Fyn Kinase Regulation by Submembrane LocalizationQ90770128
Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell SpreadingQ92142838
Structure of human Fyn kinase domain complexed with staurosporineQ40266222
A role for the tyrosine kinase Pyk2 in depolarization-induced contraction of vascular smooth muscleQ40402700
The HADDOCK2.2 Web Server: User-Friendly Integrative Modeling of Biomolecular Complexes.Q40496139
Knockouts of Src-family kinases: stiff bones, wimpy T cells, and bad memories.Q41075059
Functional and physical interaction of protein-tyrosine kinases Fyn and Csk in the T-cell signaling systemQ41508087
Identification and characterization of a novel Sso7d scaffold-based binder against Notch1.Q41696862
Inhibition of integrin-mediated crosstalk with epidermal growth factor receptor/Erk or Src signaling pathways in autophagic prostate epithelial cells induces caspase-independent deathQ41998434
Oscillatory control of factors determining multipotency and fate in mouse neural progenitorsQ42258504
High-sensitivity measurements of multiple kinase activities in live single cellsQ42735283
Integrin-mediated activation of Cdc42 controls cell polarity in migrating astrocytes through PKCzetaQ28583531
A computational approach to edge detectionQ29029687
An efficient one-step site-directed and site-saturation mutagenesis protocolQ29614689
Cellular functions regulated by Src family kinasesQ29616038
The HADDOCK web server for data-driven biomolecular dockingQ29617755
Specificity and versatility of SH3 and other proline-recognition domains: structural basis and implications for cellular signal transductionQ30014849
Structure and regulation of Src family kinasesQ30160827
Coordination of cell polarization and migration by the Rho family GTPases requires Src tyrosine kinase activityQ30167760
Dynamic coupling between the SH2 and SH3 domains of c-Src and Hck underlies their inactivation by C-terminal tyrosine phosphorylationQ30168286
Structures of Src-family tyrosine kinasesQ30176322
Inhibition of focal adhesion kinase by PF-562,271 inhibits the growth and metastasis of pancreatic cancer concomitant with altering the tumor microenvironmentQ30424344
Src family kinases, key regulators of signal transductionQ30434839
Src family protein tyrosine kinases: cooperating with growth factor and adhesion signaling pathwaysQ30470153
Structure and Position of the N-Terminal Membrane-Binding Domain of pp60src at the Membrane InterfaceQ30471897
Caveolin-1 regulates cell polarization and directional migration through Src kinase and Rho GTPasesQ30480443
Determination of hierarchical relationship of Src and Rac at subcellular locations with FRET biosensors.Q30484062
Rapid and sustained nuclear-cytoplasmic ERK oscillations induced by epidermal growth factorQ30493239
A biosensor generated via high-throughput screening quantifies cell edge Src dynamicsQ30502428
Mechanical feedback through E-cadherin promotes direction sensing during collective cell migrationQ30585627
Frequency-Modulated Pulses of ERK Activity Transmit Quantitative Proliferation SignalsQ30586789
Open source software for quantification of cell migration, protrusions, and fluorescence intensitiesQ30638871
Improving 3D structure prediction from chemical shift dataQ30659119
FRET binding antenna reports spatiotemporal dynamics of GDI-Cdc42 GTPase interactionsQ30813417
Fluorescence resonance energy transfer from cyan to yellow fluorescent protein detected by acceptor photobleaching using confocal microscopy and a single laser.Q30881966
Fyn tyrosine kinase regulates oligodendroglial cell development but is not required for morphological differentiation of oligodendrocytesQ31913120
Quantitative Fluorescence Resonance Energy Transfer Measurements Using Fluorescence MicroscopyQ32063501
Visualizing the mechanical activation of Src.Q33214304
Detecting protein-protein interactions with CFP-YFP FRET by acceptor photobleachingQ33366762
Saracatinib as a metastasis inhibitor in metastatic castration-resistant prostate cancer: A University of Chicago Phase 2 Consortium and DOD/PCF Prostate Cancer Clinical Trials Consortium StudyQ33426973
Potential positive and negative autoregulation of p60c-src by intermolecular autophosphorylationQ33580628
An integrin-contactin complex regulates CNS myelination by differential Fyn phosphorylationQ33597124
Src family tyrosine kinases and growth factor signalingQ33810017
Highly stable binding proteins derived from the hyperthermophilic Sso7d scaffold.Q33880446
Protein selection using yeast surface displayQ34215858
Ultradian oscillations and pulses: coordinating cellular responses and cell fate decisionsQ34341432
Design of pH Sensitive Binding Proteins from the Hyperthermophilic Sso7d ScaffoldQ34473740
Src-family tyrosine kinase activities are essential for differentiation of human embryonic stem cells.Q34616649
Translation of myelin basic protein mRNA in oligodendrocytes is regulated by integrin activation and hnRNP-K.Q34637839
Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmidsQ35062969
Development of an optimized backbone of FRET biosensors for kinases and GTPasesQ35579824
Src kinases: a hub for NMDA receptor regulationQ35701114
Involvement of Fyn kinase in Kit and integrin-mediated Rac activation, cytoskeletal reorganization, and chemotaxis of mast cells.Q35804427
Dynamics of TGF-β signaling reveal adaptive and pulsatile behaviors reflected in the nuclear localization of transcription factor Smad4Q36094431
A Src inhibitor regulates the cell cycle of human pluripotent stem cells and improves directed differentiation.Q36102768
Src family kinases differentially influence glioma growth and motilityQ36218089
Dual fatty acylation of p59(Fyn) is required for association with the T cell receptor zeta chain through phosphotyrosine-Src homology domain-2 interactionsQ36256449
Focal adhesion kinase mediates the integrin signaling requirement for growth factor activation of MAP kinaseQ36294784
Osteopetrosis in Src-deficient mice is due to an autonomous defect of osteoclasts.Q36301037
Phosphorylation of DCC by Fyn mediates Netrin-1 signaling in growth cone guidanceQ36322746
Substrate rigidity and force define form through tyrosine phosphatase and kinase pathwaysQ36418286
Visualization of Compartmentalized Kinase Activity Dynamics Using Adaptable BimKARsQ36909070
SRC family kinases as novel therapeutic targets to treat breast cancer brain metastases.Q37193756
Adhesion molecules in the regulation of CNS myelination.Q37218958
PINE-SPARKY: graphical interface for evaluating automated probabilistic peak assignments in protein NMR spectroscopyQ37295089
Src as a therapeutic target in men with prostate cancer and bone metastasesQ37372787
Adhesion signaling - crosstalk between integrins, Src and Rho.Q37429375
Grb2 promotes integrin-induced focal adhesion kinase (FAK) autophosphorylation and directs the phosphorylation of protein tyrosine phosphatase α by the Src-FAK kinase complexQ37546973
Examining Crosstalk among Transforming Growth Factor β, Bone Morphogenetic Protein, and Wnt PathwaysQ37567224
Src kinases as therapeutic targets for cancerQ37604891
Fyn kinase inhibition as a novel therapy for Alzheimer's diseaseQ37689640
Feedback regulation of EGFR signalling: decision making by early and delayed loops.Q37831109
Fyn Kinase in Brain Diseases and Cancer: The Search for InhibitorsQ37886227
Finding the weakest link: exploring integrin-mediated mechanical molecular pathwaysQ38026498
c-Src, Insulin-Like Growth Factor I Receptor, G-Protein-Coupled Receptor Kinases and Focal Adhesion Kinase are Enriched Into Prostate Cancer Cell ExosomesQ38768131
KLF5 promotes cell migration by up-regulating FYN in bladder cancer cells.Q38801627
Receptor Level Mechanisms Are Required for Epidermal Growth Factor (EGF)-stimulated Extracellular Signal-regulated Kinase (ERK) Activity PulsesQ38841110
Basic fibroblast growth factor regulates persistent ERK oscillations in premalignant but not malignant JB6 cells.Q39762244
Protein Tyrosine Kinase Fyn Regulates TLR4-Elicited Responses on Mast Cells Controlling the Function of a PP2A-PKCα/β Signaling Node Leading to TNF Secretion.Q39763193
NCAM-induced focal adhesion assembly: a functional switch upon loss of E-cadherinQ39943000
P4510describes a project that usesImageJQ1659584
P577publication date2020-02-04
P1433published ineLifeQ2000008
P1476titleA Fyn biosensor reveals pulsatile, spatially localized kinase activity and signaling crosstalk in live mammalian cells
P478volume9

Reverse relations

cites work (P2860)
Q97438432Focal adhesion displacement magnitude is a unifying feature of tensional homeostasis
Q108591280Two Motors and One Spring: Hypothetic Roles of Non-Muscle Myosin II and Submembrane Actin-Based Cytoskeleton in Cell Volume Sensing

Search more.