The structural biology of enzymes involved in natural product glycosylation

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The structural biology of enzymes involved in natural product glycosylation is …
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scholarly articleQ13442814

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P356DOI10.1039/C2NP20039B
P932PMC publication ID3627186
P698PubMed publication ID22688446

P50authorGeorge N PhillipsQ95337388
Shanteri SinghQ41112372
Jon S ThorsonQ41112392
P2860cites workA genomic overview of pyridoxal-phosphate-dependent enzymesQ24536053
Structure and function of both domains of ArnA, a dual function decarboxylase and a formyltransferase, involved in 4-amino-4-deoxy-L-arabinose biosynthesisQ24596697
Biochemical and structural characterization of WlbA from Bordetella pertussis and Chromobacterium violaceum: enzymes required for the biosynthesis of 2,3-diacetamido-2,3-dideoxy-D-mannuronic acidQ24606876
Structure and mechanism of ArnA: conformational change implies ordered dehydrogenase mechanism in key enzyme for polymyxin resistanceQ24630632
A structural basis for the allosteric regulation of non-hydrolysing UDP-GlcNAc 2-epimerasesQ24646011
Crystal structure of Escherichia coli ArnA (PmrI) decarboxylase domain. A key enzyme for lipid A modification with 4-amino-4-deoxy-L-arabinose and polymyxin resistanceQ24646087
Structure and mechanism of the rebeccamycin sugar 4'-O-methyltransferase RebMQ24646712
Crystal structure of the bifunctional N-acetylglucosamine 1-phosphate uridyltransferase from Escherichia coli: a paradigm for the related pyrophosphorylase superfamilyQ27619240
Structural and kinetic analysis of Escherichia coli GDP-mannose 4,6 dehydratase provides insights into the enzyme's catalytic mechanism and regulation by GDP-fucoseQ27621354
Redesign of polyene macrolide glycosylation: engineered biosynthesis of 19-(O)-perosaminyl-amphoteronolide B.Q43147546
Molecular mechanisms of fosfomycin resistance in clinical isolates of Escherichia coliQ43195414
Biological mechanisms involved in the formation of deoxy sugars. IV. Enzymatic conversion of thymidine diphosphoglucose-4T to thymidine diphospho-4-keto-6-deoxyglucose-6T.Q43522503
Asparagine 23 and aspartate 305 are essential residues in the active site of UDP-N-acetylglucosamine enolpyruvyl transferase from Enterobacter cloacaeQ43591572
Mechanistic investigation of UDP-galactopyranose mutase from Escherichia coli using 2- and 3-fluorinated UDP-galactofuranose as probesQ43670133
Bacterial modification of LPS and resistance to antimicrobial peptidesQ43718689
Regioselectivity of catechol O-methyltransferase. The effect of pH on the site of O-methylation of fluorinated norepinephrinesQ43843613
Genetic engineering of aminodeoxyhexose biosynthesis in Streptomyces fradiaeQ44044947
On the origin of deoxypentoses: evidence to support a glucose progenitor in the biosynthesis of calicheamicinQ44197156
Role of the loop containing residue 115 in the induced-fit mechanism of the bacterial cell wall biosynthetic enzyme MurAQ27621570
RmlC, the third enzyme of dTDP-L-rhamnose pathway, is a new class of epimeraseQ27622479
The crystal structure of ADP-L-glycero-D-mannoheptose 6-epimerase: catalysis with a twistQ27625372
Probing the catalytic mechanism of GDP-4-keto-6-deoxy-d-mannose Epimerase/Reductase by kinetic and crystallographic characterization of site-specific mutantsQ27627359
The structure of UDP-N-acetylglucosamine 2-epimerase reveals homology to phosphoglycosyl transferasesQ27628806
The structural basis of the catalytic mechanism and regulation of glucose-1-phosphate thymidylyltransferase (RmlA)Q27628928
Crystal structure of Streptococcus pneumoniae N-acetylglucosamine-1-phosphate uridyltransferase bound to acetyl-coenzyme A reveals a novel active site architectureQ27628944
Crystal structures of Streptococcus pneumoniae N-acetylglucosamine-1-phosphate uridyltransferase, GlmU, in apo form at 2.33 A resolution and in complex with UDP-N-acetylglucosamine and Mg(2+) at 1.96 A resolutionQ27629008
The crystal structure of dTDP-D-Glucose 4,6-dehydratase (RmlB) from Salmonella enterica serovar Typhimurium, the second enzyme in the dTDP-l-rhamnose pathwayQ27630554
Structure, mechanism and engineering of a nucleotidylyltransferase as a first step toward glycorandomizationQ27632060
UDP-galactopyranose mutase has a novel structure and mechanismQ27635026
Kinetic and crystallographic analyses support a sequential-ordered bi bi catalytic mechanism for Escherichia coli glucose-1-phosphate thymidylyltransferaseQ27636040
Toward a structural understanding of the dehydratase mechanismQ27637363
Variation on a theme of SDR. dTDP-6-deoxy-L- lyxo-4-hexulose reductase (RmlD) shows a new Mg2+-dependent dimerization modeQ27639149
Purification, crystallization and preliminary structural studies of dTDP-4-keto-6-deoxy-glucose-5-epimerase (EvaD) from Amycolatopsis orientalis, the fourth enzyme in the dTDP-L-epivancosamine biosynthetic pathwayQ27639207
Crystal structure of Escherichia coli glucose-1-phosphate thymidylyltransferase (RffH) complexed with dTTP and Mg2+Q27639485
Expanding pyrimidine diphosphosugar libraries via structure-based nucleotidylyltransferase engineeringQ27639760
Structural studies of Salmonella typhimurium ArnB (PmrH) aminotransferase: a 4-amino-4-deoxy-L-arabinose lipopolysaccharide-modifying enzymeQ27639956
High resolution x-ray structure of tyvelose epimerase from Salmonella typhiQ27640717
Structure and Dynamics of UDP–Glucose Pyrophosphorylase from Arabidopsis thaliana with Bound UDP–Glucose and UTPQ27640844
High-resolution structures of RmlC from Streptococcus suis in complex with substrate analogs locate the active site of this class of enzymeQ27641399
A new view of the mechanisms of UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) and 5-enolpyruvylshikimate-3-phosphate synthase (AroA) derived from X-ray structures of their tetrahedral reaction intermediate statesQ27642065
The structure of NADH in the enzyme dTDP-d-glucose dehydratase (RmlB)Q27642216
High resolution X-ray structure of dTDP-glucose 4,6-dehydratase from Streptomyces venezuelaeQ27642390
Crystal structure of a tetrameric GDP-D-mannose 4,6-dehydratase from a bacterial GDP-D-rhamnose biosynthetic pathwayQ27643014
The molecular architecture of glucose-1-phosphate uridylyltransferaseQ27643901
The Complex of Sphingomonas elodea ATCC 31461 Glucose-1-Phosphate Uridylyltransferase with Glucose-1-Phosphate Reveals a Novel Quaternary Structure, Unique among Nucleoside Diphosphate-Sugar Pyrophosphorylase MembersQ27644458
Molecular architecture of DesV fromStreptomyces venezuelae: A PLP-dependent transaminase involved in the biosynthesis of the unusual sugar desosamineQ27644595
The x-ray structure of dTDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomeraseQ27644605
Structure of theE. colibifunctional GlmU acetyltransferase active site with substrates and productsQ27644659
Active site geometry of glucose-1-phosphate uridylyltransferaseQ27645792
Molecular Architecture of DesI:  A Key Enzyme in the Biosynthesis of Desosamine † , ‡Q27646687
A Structural Study of GDP-4-Keto-6-Deoxy- d -Mannose-3-Dehydratase:  Caught in the Act of Geminal Diamine Formation † , ‡Q27649009
Characterization of substrate binding and catalysis in the potential antibacterial targetN-acetylglucosamine-1-phosphate uridyltransferase (GlmU)Q27649118
Enzymatic methods for glyco(diversification/randomization) of drugs and small moleculesQ28247479
Evolutionary trace analysis of the alpha-D-phosphohexomutase superfamilyQ28270568
Structure and functions of the GNAT superfamily of acetyltransferasesQ28296840
Mycobacterium tuberculosis RmlC epimerase (Rv3465): a promising drug-target structure in the rhamnose pathwayQ28486876
RmlC, a C3' and C5' carbohydrate epimerase, appears to operate via an intermediate with an unusual twist boat conformationQ28490087
Many paths to methyltransfer: a chronicle of convergenceQ29616667
SAM (dependent) I AM: the S-adenosylmethionine-dependent methyltransferase foldQ29618153
GCN5-related N-acetyltransferases: a structural overviewQ29619219
Pyridoxal phosphate enzymes: mechanistic, structural, and evolutionary considerationsQ29620045
Short-chain dehydrogenases/reductases (SDR)Q29620095
Structure-based phylogeny as a diagnostic for functional characterization of proteins with a cupin foldQ30157239
Evolution of functional diversity in the cupin superfamily.Q30167749
Structural and mechanistic basis of bacterial sugar nucleotide-modifying enzymes.Q30333015
Natural-product sugar biosynthesis and enzymatic glycodiversificationQ30492476
Characterization of enzymatic processes by rapid mix-quench mass spectrometry: the case of dTDP-glucose 4,6-dehydratase.Q30961494
Molecular diversity through sugar scaffoldsQ30977448
Dehydration is catalyzed by glutamate-136 and aspartic acid-135 active site residues in Escherichia coli dTDP-glucose 4,6-dehydrataseQ31016682
Sweet antibiotics - the role of glycosidic residues in antibiotic and antitumor activity and their randomizationQ33353798
Carbohydrate scaffolds in chemical genetic studiesQ33470834
Combinatorial biosynthesis for new drug discoveryQ33538629
Carbohydrate mimetics and scaffolds: sweet spots in medicinal chemistryQ33852360
Crystal structure of potato tuber ADP-glucose pyrophosphorylaseQ33868406
Structure and function of enzymes of the Leloir pathway for galactose metabolismQ33967795
Galactokinase: structure, function and role in type II galactosemiaQ33982846
Molecular structure of human galactokinase: implications for type II galactosemiaQ33983696
Genetic and functional analysis of a PmrA-PmrB-regulated locus necessary for lipopolysaccharide modification, antimicrobial peptide resistance, and oral virulence of Salmonella enterica serovar typhimuriumQ34004929
The rhamnose pathwayQ34103415
Pathways and mechanisms in the biogenesis of novel deoxysugars by bacteriaQ34316342
A unique catalytic mechanism for UDP-galactopyranose mutaseQ34319532
The mechanism of the reaction catalyzed by ADP-beta-L-glycero-D-manno-heptose 6-epimeraseQ34334649
Chemical insights from structural studies of enzymesQ34360265
Glycosides in medicine: "The role of glycosidic residue in biological activity".Q34372899
Reaction specificity in pyridoxal phosphate enzymesQ34373891
A formyltransferase required for polymyxin resistance in Escherichia coli and the modification of lipid A with 4-Amino-4-deoxy-L-arabinose. Identification and function oF UDP-4-deoxy-4-formamido-L-arabinoseQ34391736
Crystal structure and mechanism of the Escherichia coli ArnA (PmrI) transformylase domain. An enzyme for lipid A modification with 4-amino-4-deoxy-L-arabinose and polymyxin resistanceQ34408515
GDP-4-keto-6-deoxy-D-mannose 3-dehydratase, accommodating a sugar substrate in the active siteQ27649164
Structure and active site residues of PglD, an N-acetyltransferase from the bacillosamine synthetic pathway required for N-glycan synthesis in Campylobacter jejuniQ27649543
Crystal structure of UDP-N-acetylglucosamine enolpyruvyl transferase from Haemophilus influenzae in complex with UDP-N-acetylglucosamine and fosfomycinQ27649770
GDP-perosamine synthase: structural analysis and production of a novel trideoxysugarQ27649775
Three-dimensional structure of DesVI from Streptomyces venezuelae: a sugar N,N-dimethyltransferase required for dTDP-desosamine biosynthesisQ27650032
Structural analysis of ADP-glucose pyrophosphorylase from the bacterium Agrobacterium tumefaciensQ27650121
Crystal Structure and Catalytic Mechanism of PglD from Campylobacter jejuniQ27651382
Accommodation of GDP-linked sugars in the active site of GDP-perosamine synthaseQ27652172
Structural Analysis of QdtB, an Aminotransferase Required for the Biosynthesis of dTDP-3-acetamido-3,6-dideoxy-α- d -glucose † ‡Q27653599
Structural and Functional Studies of QdtC: An N -Acetyltransferase Required for the Biosynthesis of dTDP-3-Acetamido-3,6-dideoxy-α- d -glucose † ‡Q27653643
Structure and function of GlmU fromMycobacterium tuberculosisQ27653924
Two Site-Directed Mutations Are Required for the Conversion of a Sugar Dehydratase into an Aminotransferase §Q27655325
Structure ofN-acetylglucosamine-1-phosphate uridyltransferase (GlmU) fromMycobacterium tuberculosisin a cubic space groupQ27655339
Ligand Binding and Substrate Discrimination by UDP-Galactopyranose MutaseQ27655753
X-ray crystallography reveals a reduced substrate complex of UDP-galactopyranose mutase poised for covalent catalysis by flavinQ27657238
Structural basis of substrate binding to UDP-galactopyranose mutase: crystal structures in the reduced and oxidized state complexed with UDP-galactopyranose and UDPQ27657821
The Crystal Structure of the Novobiocin Biosynthetic Enzyme NovP: The First Representative Structure for the TylF O-Methyltransferase SuperfamilyQ27657980
Evidence of kinetic control of ligand binding and staged product release in MurA (enolpyruvyl UDP-GlcNAc synthase)-catalyzed reactionsQ27658111
Structural basis for the reaction mechanism of UDP-glucose pyrophosphorylaseQ27660270
X-ray structure of kijd3, a key enzyme involved in the biosynthesis of D-kijanoseQ27660314
Structural and mechanistic basis for a new mode of glycosyltransferase inhibitionQ27660415
Molecular Structure of WlbB, a Bacterial N -Acetyltransferase Involved in the Biosynthesis of 2,3-Diacetamido-2,3-dideoxy- d -mannuronic Acid,Q27661069
The crystal structure of the Y140F mutant of ADP-L-glycero-D-manno-heptose 6-epimerase bound to ADP-β-D-mannose suggests a one base mechanismQ27661977
Molecular architecture of a C-3'-methyltransferase involved in the biosynthesis of D-tetronitroseQ27662124
Structural Insights into the Catalytic Mechanism of Bacterial Guanosine-diphospho-D-mannose Pyrophosphorylase and Its Regulation by Divalent IonsQ27662663
Structural Analysis of WbpE from Pseudomonas aeruginosa PAO1: A Nucleotide Sugar Aminotransferase Involved in O-Antigen Assembly,Q27663161
Chemoenzymatic synthesis, inhibition studies, and X-ray crystallographic analysis of the phosphono analog of UDP-Galp as an inhibitor and mechanistic probe for UDP-galactopyranose mutaseQ27664551
Structure and mechanism of ORF36, an amino sugar oxidizing enzyme in everninomicin biosynthesis .Q27664658
Molecular Architecture of TylM1 from Streptomyces fradiae : An N , N -Dimethyltransferase Involved in the Production of dTDP- d -mycaminose,Q27666229
Expanding the Nucleotide and Sugar 1-Phosphate Promiscuity of Nucleotidyltransferase RmlA via Directed EvolutionQ27666946
Altered architecture of substrate binding region defines the unique specificity of UDP-GalNAc 4-epimerasesQ27667219
Combined structural and functional investigation of a C-3''-ketoreductase involved in the biosynthesis of dTDP-L-digitoxoseQ27667911
A New Structural Form in the SAM/Metal-Dependent O‑Methyltransferase Family: MycE from the Mycinamicin Biosynthetic PathwayQ27672783
The crystal structure of ADP-L-glycero-D-manno-heptose-6-epimerase (HP0859) from Helicobacter pyloriQ27674767
Structural studies of AntD: an N-Acyltransferase involved in the biosynthesis of D-AnthroseQ27676557
A left-handed parallel beta helix in the structure of UDP-N-acetylglucosamine acyltransferaseQ27729303
Towards structure-based drug design: crystal structure of a multisubstrate adduct complex of glycinamide ribonucleotide transformylase at 1.96 A resolutionQ27730263
The structure of glucose-fructose oxidoreductase from Zymomonas mobilis: an osmoprotective periplasmic enzyme containing non-dissociable NADPQ27734347
Structure of UDP-N-acetylglucosamine enolpyruvyl transferase, an enzyme essential for the synthesis of bacterial peptidoglycan, complexed with substrate UDP-N-acetylglucosamine and the drug fosfomycinQ27734363
Structural analysis of the H166G site-directed mutant of galactose-1-phosphate uridylyltransferase complexed with either UDP-glucose or UDP-galactose: detailed description of the nucleotide sugar binding siteQ27734963
Structural analysis of UDP-sugar binding to UDP-galactose 4-epimerase from Escherichia coliQ27738620
Mechanistic roles of tyrosine 149 and serine 124 in UDP-galactose 4-epimerase from Escherichia coliQ27742332
Dramatic differences in the binding of UDP-galactose and UDP-glucose to UDP-galactose 4-epimerase from Escherichia coliQ27765151
GDP-4-keto-6-deoxy-D-mannose epimerase/reductase from Escherichia coli, a key enzyme in the biosynthesis of GDP-L-fucose, displays the structural characteristics of the RED protein homology superfamilyQ27766039
GDP-fucose synthetase from Escherichia coli: structure of a unique member of the short-chain dehydrogenase/reductase family that catalyzes two distinct reactions at the same active siteQ27766387
Formation of unusual sugars: mechanistic studies and biosynthetic applicationsQ28202326
Mechanistic roles of Thr134, Tyr160, and Lys 164 in the reaction catalyzed by dTDP-glucose 4,6-dehydrataseQ28209829
The calicheamicin gene cluster and its iterative type I enediyne PKSQ28218405
Glycosyltransferase structural biology and its role in the design of catalysts for glycosylationQ28238053
The mechanism of 6-deoxyhexose synthesis. I. Intramolecular hydrogen transfer catalyzed by deoxythymidine diphosphate D-glucose oxidoreductaseQ28246656
Reconstitution of UDP-galactopyranose mutase with 1-deaza-FAD and 5-deaza-FAD: analysis and mechanistic implicationsQ44653012
The position of a key tyrosine in dTDP-4-Keto-6-deoxy-D-glucose-5-epimerase (EvaD) alters the substrate profile for this RmlC-like enzyme.Q44907831
Active site mutants of the "non-hydrolyzing" UDP-N-acetylglucosamine 2-epimerase from Escherichia coliQ44946645
Molecular structure of alpha-D-glucose-1-phosphate cytidylyltransferase from Salmonella typhiQ45005968
Sch 37137, a novel antifungal compound produced by a Micromonospora sp. Taxonomy, fermentation, isolation, structure and biological properties.Q45167698
Unusually broad substrate tolerance of a heat-stable archaeal sugar nucleotidyltransferase for the synthesis of sugar nucleotidesQ45177620
Characterization of CalS9 in the biosynthesis of UDP-xylose and the production of xylosyl-attached hybrid compoundQ45375180
The molecular architecture of galactose mutarotase/UDP-galactose 4-epimerase from Saccharomyces cerevisiaeQ46408194
Dismutase activity of ADP-L-glycero-D-manno-heptose 6-epimerase: evidence for a direct oxidation/reduction mechanismQ46431933
Structural analysis of a set of proteins resulting from a bacterial genomics project.Q46445101
Genetically engineered biosynthesis of macrolide derivatives including 4-amino-4,6-dideoxy-L-glucose from Streptomyces venezuelae YJ003-OTBP3.Q46779416
Differential role of NADP+ and NADPH in the activity and structure of GDP-D-mannose 4,6-dehydratase from two chlorella viruses.Q46921021
Insights into the Branched-Chain Formation of Mycarose: Methylation Catalyzed by an (S)-Adenosylmethionine-Dependent Methyltransferase We are grateful to Dr. Eugene Seno and the Lilly Research Laboratories for their generous gift of the plasmid pHJLQ48370425
Mechanistic implications of Escherichia coli galactokinase structure-based engineering.Q51651257
Reaction of aspartate aminotransferase with L-erythro-3-hydroxyaspartate: involvement of Tyr70 in stabilization of the catalytic intermediates.Q52335636
A rapid screen of active site mutants in glycinamide ribonucleotide transformylase.Q54585483
Characterization of a Cys115 to Asp substitution in the Escherichia coli cell wall biosynthetic enzyme UDP-GlcNAc enolpyruvyl transferase (MurA) that confers resistance to inactivation by the antibiotic fosfomycin.Q54590227
Variation on a Theme of SDRQ57278413
Characterisation of Streptomyces spheroides NovW and revision of its functional assignment to a dTDP-6-deoxy-d-xylo-4-hexulose 3-epimeraseQ57572222
The 1.6-Å resolution crystal structure of NovW: A 4-keto-6-deoxy sugar epimerase from the novobiocin biosynthetic gene cluster of Streptomyces spheroidesQ57572230
Lysine 22 in UDP-N-Acetylglucosamine Enolpyruvyl Transferase fromEnterobacter cloacaeIs Crucial for Enzymatic Activity and the Formation of Covalent Adducts with the Substrate Phosphoenolpyruvate and the Antibiotic Fosfomycin†Q57978316
Chemical modification of everninomicinsQ70429388
Uridine diphosphate N-acetyl-D-glucosamine-2-epimerase from rat liver. II. Studies on the mechanism of actionQ70457894
Biosynthesis of the anti-parasitic agent megalomicin: transformation of erythromycin to megalomicin in Saccharopolyspora erythraeaQ74266942
On the mechanism of N-acetylmannosamine formationQ79252452
Crystallization and preliminary X-ray crystallographic study of UDP-glucose pyrophosphorylase (UGPase) from Helicobacter pyloriQ80375808
Insights into the Branched-Chain Formation of Mycarose: Methylation Catalyzed by an (S)-Adenosylmethionine-Dependent MethyltransferaseQ88522050
Diversifying vancomycin via chemoenzymatic strategies.Q34409603
Structures and mechanisms of glycosyltransferasesQ34436062
Epimerases: structure, function and mechanismQ34437812
Functional characterization of dehydratase/aminotransferase pairs from Helicobacter and Campylobacter: enzymes distinguishing the pseudaminic acid and bacillosamine biosynthetic pathways.Q34467309
Structural and functional characterization of PseC, an aminotransferase involved in the biosynthesis of pseudaminic acid, an essential flagellar modification in Helicobacter pyloriQ34485821
Structure, evolution and action of vitamin B6-dependent enzymesQ34488807
Structural studies of FlaA1 from Helicobacter pylori reveal the mechanism for inverting 4,6-dehydratase activityQ34519878
Crystal structure of TDP-fucosamine acetyltransferase (WecD) from Escherichia coli, an enzyme required for enterobacterial common antigen synthesisQ34549307
Deciphering indolocarbazole and enediyne aminodideoxypentose biosynthesis through comparative genomics: insights from the AT2433 biosynthetic locus.Q34551703
The molecular structure of UDP-galactose 4-epimerase from Escherichia coli determined at 2.5 A resolutionQ34556266
Stereospecificity for the hydrogen transfer of pyridoxal enzyme reactionsQ34587643
Unusual sugar biosynthesis and natural product glycodiversificationQ34623021
Biosynthetic enzymes of unusual microbial sugarsQ34626027
Expanding the promiscuity of a natural-product glycosyltransferase by directed evolutionQ34683354
Mechanistic aspects of enzymatic carbohydrate epimerizationQ34757304
The impact of enzyme engineering upon natural product glycodiversificationQ34803952
Mechanisms and structures of vitamin B(6)-dependent enzymes involved in deoxy sugar biosynthesisQ35046495
A structural perspective on the enzymes that convert dTDP-d-glucose into dTDP-l-rhamnoseQ35139330
Natural product glycorandomization.Q35758002
Nucleophilic participation of reduced flavin coenzyme in mechanism of UDP-galactopyranose mutaseQ35762957
Bioactive carbohydrates and recently discovered analogues as chemotherapeuticsQ35768540
The role of natural product chemistry in drug discoveryQ35994110
CDP-6-deoxy-delta 3,4-glucoseen reductase from Yersinia pseudotuberculosis: enzyme purification and characterization of the cloned geneQ36104792
Glycosyltransferases: managers of small moleculesQ36110231
Enzymatic tools for engineering natural product glycosylation.Q36468505
Inferring the chemical mechanism from structures of enzymesQ36579441
Engineering the glycosylation of natural products in actinomycetesQ36782462
Elucidation of the kijanimicin gene cluster: insights into the biosynthesis of spirotetronate antibiotics and nitrosugarsQ36835457
Increasing carbohydrate diversity via amine oxidation: aminosugar, hydroxyaminosugar, nitrososugar, and nitrosugar biosynthesis in bacteriaQ36839243
Carbohydrates in therapeuticsQ36881558
Discovery and molecular engineering of sugar-containing natural product biosynthetic pathways in actinomycetes.Q37048281
Recent knowledge and innovations related to hexofuranosides: structure, synthesis and applications.Q37148970
Glycosyltransferases, important tools for drug designQ37161024
Characterization of CalE10, the N-oxidase involved in calicheamicin hydroxyaminosugar formationQ37247384
???Q27748883
Galactose metabolism in yeast-structure and regulation of the leloir pathway enzymes and the genes encoding themQ37263636
Structure and mutagenic conversion of E1 dehydrase: at the crossroads of dehydration, amino transfer, and epimerizationQ37436403
Investigation of binding of UDP-Galf and UDP-[3-F]Galf to UDP-galactopyranose mutase by STD-NMR spectroscopy, molecular dynamics, and CORCEMA-ST calculationsQ37457660
A bacterial glycosyltransferase gene toolbox: generation and applicationsQ37533001
Chemistry and biology of galactofuranose-containing polysaccharidesQ37547089
Structure, mechanism and engineering of plant natural product glycosyltransferasesQ37606691
Glycosyltransferases and their AssaysQ37776670
Dynamics of glucosamine-6-phosphate synthase catalysisQ37779930
Glycosyltransferases as biocatalystsQ37844959
Evidence that the fosfomycin target Cys115 in UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) is essential for product releaseQ37866109
UDP-N-acetylglucosamine enolpyruvyl transferase as a potential target for antibacterial chemotherapy: recent developmentsQ37913126
Discovery and design of carbohydrate-based therapeuticsQ38029137
Site-directed mutagenesis of UDP-galactopyranose mutase reveals a critical role for the active-site, conserved arginine residuesQ38301618
Enhancing the latent nucleotide triphosphate flexibility of the glucose-1-phosphate thymidylyltransferase RmlA.Q38302744
Cell wall core galactofuran synthesis is essential for growth of mycobacteriaQ39503992
Structure of CDP-D-glucose 4,6-dehydratase from Salmonella typhi complexed with CDP-D-xyloseQ41458900
Identification of an unusual [2Fe-2S]-binding motif in the CDP-6-deoxy-D-glycero-l-threo-4-hexulose-3-dehydrase from Yersinia pseudotuberculosis: implication for C-3 deoxygenation in the biosynthesis of 3,6-dideoxyhexosesQ41460846
Crystal structure at 1.8 A resolution of CDP-D-glucose 4,6-dehydratase from Yersinia pseudotuberculosisQ41463453
The biosynthesis of GDP-L-colitose: C-3 deoxygenation is catalyzed by a unique coenzyme B6-dependent enzymeQ41467641
The role of carbohydrates in biologically active natural productsQ41468708
Kinetic characterization of an organic radical in the ascarylose biosynthetic pathwayQ41489921
Cofactor characterization and mechanistic studies of CDP-6-deoxy-delta 3,4-glucoseen reductase: exploration into a novel enzymatic C-O bond cleavage eventQ41501451
The structure of GDP-4-keto-6-deoxy-D-mannose-3-dehydratase: a unique coenzyme B6-dependent enzymeQ41842380
Crystal structures of Mycobacteria tuberculosis and Klebsiella pneumoniae UDP-galactopyranose mutase in the oxidised state and Klebsiella pneumoniae UDP-galactopyranose mutase in the (active) reduced stateQ41878559
A unifying nitrososynthase involved in nitrosugar biosynthesisQ41960421
Synthesis and analysis of substrate analogues for UDP-galactopyranose mutase: implication for an oxocarbenium ion intermediate in the catalytic mechanismQ42390505
Evolutionarily conserved regions and hydrophobic contacts at the superfamily level: The case of the fold-type I, pyridoxal-5'-phosphate-dependent enzymesQ42638678
Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061.Q42639702
Kinetic and structural analysis of alpha-D-Glucose-1-phosphate cytidylyltransferase from Salmonella typhiQ42644312
P433issue10
P407language of work or nameEnglishQ1860
P921main subjectglycosylationQ898365
natural productQ901227
structural biologyQ908902
drug discoveryQ1418791
organic chemistryQ11351
P304page(s)1201-1237
P577publication date2012-06-12
P1433published inNatural Product ReportsQ2376904
P1476titleThe structural biology of enzymes involved in natural product glycosylation
P478volume29

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Q36137644Characterization of Early Enzymes Involved in TDP-Aminodideoxypentose Biosynthesis en Route to Indolocarbazole AT2433.
Q91643308Cryo-EM structure of L-fucokinase/GDP-fucose pyrophosphorylase (FKP) in Bacteroides fragilis
Q31811695Crystal Structure of the N-Acetylmuramic Acid α-1-Phosphate (MurNAc-α1-P) Uridylyltransferase MurU, a Minimal Sugar Nucleotidyltransferase and Potential Drug Target Enzyme in Gram-negative Pathogens
Q48203787Crystal structure of a UDP-GlcNAc epimerase for surface polysaccharide biosynthesis in Acinetobacter baumannii
Q64953997Discovery of an RmlC/D fusion protein in the microalga Prymnesium parvum and its implications for NDP-β-l-rhamnose biosynthesis in microalgae.
Q44927649Discovery of tanshinone derivatives with anti-MRSA activity via targeted bio-transformation.
Q38429805Enhanced production of nargenicin A(1) and generation of novel glycosylated derivatives.
Q58772177Enzymatic Synthesis of the C-Glycosidic Moiety of Nogalamycin R
Q34702005Enzymatic methylation and structure-activity-relationship studies on polycarcin V, a gilvocarcin-type antitumor agent
Q33737744Enzymatic synthesis of epothilone A glycosides
Q30993753Enzymatic synthesis of novel isobavachalcone glucosides via a UDP-glycosyltransferase
Q37340715Glycogenomics as a mass spectrometry-guided genome-mining method for microbial glycosylated molecules
Q34548172Identification of Neuroprotective Spoxazomicin and Oxachelin Glycosides via Chemoenzymatic Glycosyl-Scanning
Q54309024Kinetic evaluation of glucose 1-phosphate analogues with a thymidylyltransferase using a continuous coupled enzyme assay.
Q36599498Loop dynamics of thymidine diphosphate-rhamnose 3'-O-methyltransferase (CalS11), an enzyme in calicheamicin biosynthesis
Q50676392Metabolomic Analysis of Mice Exposed to Gamma Radiation Reveals a Systemic Understanding of Total-Body Exposure.
Q33951389Neoglycosylation and neoglycorandomization: Enabling tools for the discovery of novel glycosylated bioactive probes and early stage leads
Q54292404Polyphosphate-containing bisubstrate analogues as inhibitors of a bacterial cell wall thymidylyltransferase.
Q38272215Potential applications of glucosyltransferases in terpene glucoside production: impacts on the use of aroma and fragrance
Q36074427Structural Basis for the Stereochemical Control of Amine Installation in Nucleotide Sugar Aminotransferases
Q36283579Structural Characterization of CalS8, a TDP-α-D-Glucose Dehydrogenase Involved in Calicheamicin Aminodideoxypentose Biosynthesis
Q37419993Structural and functional characterization of CalS11, a TDP-rhamnose 3'-O-methyltransferase involved in calicheamicin biosynthesis
Q35612402Structural basis of substrate specificity and regiochemistry in the MycF/TylF family of sugar O-methyltransferases.
Q36060114Structural characterization of AtmS13, a putative sugar aminotransferase involved in indolocarbazole AT2433 aminopentose biosynthesis
Q41865226Structural characterization of O- and C-glycosylating variants of the landomycin glycosyltransferase LanGT2
Q38428716Structural modification of herboxidiene by substrate-flexible cytochrome P450 and glycosyltransferase.
Q37270510Synthesis and antibacterial activity of doxycycline neoglycosides.
Q34428173The evolution of enzyme function in the isomerases

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