Structures of the RNA-guided surveillance complex from a bacterial immune system

scientific article

Structures of the RNA-guided surveillance complex from a bacterial immune system is …
instance of (P31):
scholarly articleQ13442814

External links are
P6179Dimensions Publication ID1003817588
P356DOI10.1038/NATURE10402
P932PMC publication ID4165517
P698PubMed publication ID21938068

P50authorJennifer DoudnaQ56068
Eva NogalesQ4795157
John van der OostQ42130140
Gabriel C. LanderQ50129859
Blake WiedenheftQ60169493
Stan J. J. BrounsQ90943178
Kaihong ZhouQ100151428
P2093author name stringMatthijs M Jore
P2860cites workA guild of 45 CRISPR-associated (Cas) protein families and multiple CRISPR/Cas subtypes exist in prokaryotic genomesQ21090166
A putative RNA-interference-based immune system in prokaryotes: computational analysis of the predicted enzymatic machinery, functional analogies with eukaryotic RNAi, and hypothetical mechanisms of actionQ21203780
CRISPR Provides Acquired Resistance Against Viruses in ProkaryotesQ21508827
CRISPR RNA maturation by trans-encoded small RNA and host factor RNase IIIQ24628207
Cas6 is an endoribonuclease that generates guide RNAs for invader defense in prokaryotesQ27653417
Enhancement of the Seed-Target Recognition Step in RNA Silencing by a PIWI/MID Domain ProteinQ27653626
Sequence- and Structure-Specific RNA Processing by a CRISPR EndonucleaseQ27664403
Interaction of the Cas6 Riboendonuclease with CRISPR RNAs: Recognition and CleavageQ27666877
An RNA-induced conformational change required for CRISPR RNA cleavage by the endoribonuclease Cse3Q27667849
Recognition and maturation of effector RNAs in a CRISPR interference pathwayQ27667851
SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related fieldsQ27860560
EMAN: semiautomated software for high-resolution single-particle reconstructionsQ27860772
Accurate determination of local defocus and specimen tilt in electron microscopyQ27861008
EMAN2: an extensible image processing suite for electron microscopyQ27861052
A new generation of the IMAGIC image processing systemQ28131751
Small CRISPR RNAs guide antiviral defense in prokaryotesQ28290898
The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNAQ28297640
Appion: an integrated, database-driven pipeline to facilitate EM image processingQ29614288
Automated molecular microscopy: the new Leginon systemQ29614290
Intervening sequences of regularly spaced prokaryotic repeats derive from foreign genetic elementsQ29615788
Identification of genes that are associated with DNA repeats in prokaryotesQ29615790
Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal originQ29617073
Visualizing density maps with UCSF ChimeraQ29619793
Single-particle reconstruction from EM images of helical filamentsQ30441072
SPARX, a new environment for Cryo-EM image processingQ33255164
Structural basis for CRISPR RNA-guided DNA recognition by CascadeQ34175461
Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequenceQ34190309
Virus population dynamics and acquired virus resistance in natural microbial communitiesQ34780702
RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactionsQ35064344
DoG Picker and TiltPicker: software tools to facilitate particle selection in single particle electron microscopyQ37401395
FindEM--a fast, efficient program for automatic selection of particles from electron micrographsQ47815374
P433issue7365
P407language of work or nameEnglishQ1860
P304page(s)486-489
P577publication date2011-09-21
P1433published inNatureQ180445
P1476titleStructures of the RNA-guided surveillance complex from a bacterial immune system
P478volume477

Reverse relations

cites work (P2860)
Q27000786A CRISPR method for genome engineering
Q35735544A Conserved Structural Chassis for Mounting Versatile CRISPR RNA-Guided Immune Responses
Q24669850A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
Q42005996A complex of Cas proteins 5, 6, and 7 is required for the biogenesis and stability of clustered regularly interspaced short palindromic repeats (crispr)-derived rnas (crrnas) in Haloferax volcanii
Q38860993A new group of phage anti-CRISPR genes inhibits the type I-E CRISPR-Cas system of Pseudomonas aeruginosa
Q37201214A ruler protein in a complex for antiviral defense determines the length of small interfering CRISPR RNAs
Q38593835Active site plasticity enables metal-dependent tuning of Cas5d nuclease activity in CRISPR-Cas type I-C system
Q37507652Altered stoichiometry Escherichia coli Cascade complexes with shortened CRISPR RNA spacers are capable of interference and primed adaptation
Q40176112Alternate binding modes of anti-CRISPR viral suppressors AcrF1/2 to Csy surveillance complex revealed by cryo-EM structures
Q33660852An updated evolutionary classification of CRISPR-Cas systems
Q30374731Annotation and Classification of CRISPR-Cas Systems.
Q92040652Approaches to study CRISPR RNA biogenesis and the key players involved
Q59794071Avoidance of Trinucleotide Corresponding to Consensus Protospacer Adjacent Motif Controls the Efficiency of Prespacer Selection during Primed Adaptation
Q34034778Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system.
Q96304850Bio-Layer Interferometry Analysis of the Target Binding Activity of CRISPR-Cas Effector Complexes
Q26853377Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity
Q34509196Biology and Applications of CRISPR Systems: Harnessing Nature's Toolbox for Genome Engineering
Q36603869Bipartite recognition of target RNAs activates DNA cleavage by the Type III-B CRISPR-Cas system
Q87985442CRISPR Editing in Biological and Biomedical Investigation
Q34883272CRISPR RNA binding and DNA target recognition by purified Cascade complexes from Escherichia coli
Q57036385CRISPR RNA-guided DNA cleavage by reconstituted Type I-A immune effector complexes
Q38597506CRISPR adaptive immune systems of Archaea
Q60907484CRISPR analysis suggests that small circular single-stranded DNA smacoviruses infect Archaea instead of humans
Q42128481CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled invader DNA by Cascade and Cas3
Q34378409CRISPR interference (CRISPRi) for sequence-specific control of gene expression
Q26824296CRISPR interference: a structural perspective
Q35334222CRISPR-Cas Adaptive Immune Systems of the Sulfolobales: Unravelling Their Complexity and Diversity
Q42334840CRISPR-Cas adaptive immunity and the three Rs.
Q92269691CRISPR-Cas in Streptococcus pyogenes
Q38196778CRISPR-Cas system: a powerful tool for genome engineering
Q27015486CRISPR-Cas systems and RNA-guided interference
Q61799721CRISPR-Cas systems are present predominantly on mobile genetic elements in Vibrio species
Q91767685CRISPR-Cas3 induces broad and unidirectional genome editing in human cells
Q38822073CRISPR-Cas9 technology and its application in haematological disorders
Q37079803CRISPR-Cas: evolution of an RNA-based adaptive immunity system in prokaryotes
Q34405661CRISPR-based technologies: prokaryotic defense weapons repurposed
Q34330105CRISPR-spacer integration reporter plasmids reveal distinct genuine acquisition specificities among CRISPR-Cas I-E variants of Escherichia coli
Q92567252CRISPR/Cas9 gene editing in a chicken model: current approaches and applications
Q38225327CRISPR/Cas9-Directed Genome Editing of Cultured Cells
Q37079810CRISPRTarget: bioinformatic prediction and analysis of crRNA targets.
Q42225525CRP represses the CRISPR/Cas system in Escherichia coli: evidence that endogenous CRISPR spacers impede phage P1 replication.
Q27683628Cas1–Cas2 complex formation mediates spacer acquisition during CRISPR–Cas adaptive immunity
Q89834538Cas3 Protein-A Review of a Multi-Tasking Machine
Q35950298Cas3 is a limiting factor for CRISPR-Cas immunity in Escherichia coli cells lacking H-NS.
Q92933245Cas3/I-C mediated target DNA recognition and cleavage during CRISPR interference are independent of the composition and architecture of Cascade surveillance complex
Q27670930Cas5d Protein Processes Pre-crRNA and Assembles into a Cascade-like Interference Complex in Subtype I-C/Dvulg CRISPR-Cas System
Q36342243Cas5d processes pre-crRNA and is a member of a larger family of CRISPR RNA endonucleases
Q33607132CasA mediates Cas3-catalyzed target degradation during CRISPR RNA-guided interference
Q38041299Cascade-mediated binding and bending of negatively supercoiled DNA.
Q36581178Characterization of CRISPR RNA biogenesis and Cas6 cleavage-mediated inhibition of a provirus in the haloarchaeon Haloferax mediterranei
Q35943228Characterization of the CRISPR/Cas subtype I-A system of the hyperthermophilic crenarchaeon Thermoproteus tenax
Q35620133Clustered Regularly Interspaced Short Palindromic Repeat-Dependent, Biofilm-Specific Death of Pseudomonas aeruginosa Mediated by Increased Expression of Phage-Related Genes
Q35098034Cmr4 is the slicer in the RNA-targeting Cmr CRISPR complex
Q36692751Codon Bias as a Means to Fine-Tune Gene Expression
Q42319553Commentary: Type I CRISPR-Cas targets endogenous genes and regulates virulence to evade mammalian host immunity
Q36093637Comparative genomic and transcriptional analyses of CRISPR systems across the genus Pyrobaculum.
Q26829917Comparative genomics of defense systems in archaea and bacteria
Q47648610Conformational Dynamics of DNA Binding and Cas3 Recruitment by the CRISPR RNA-Guided Cascade Complex
Q37277585Control of gene expression by CRISPR-Cas systems
Q57344149Cross-cleavage activity of Cas6b in crRNA processing of two different CRISPR-Cas systems in Methanosarcina mazei Gö1
Q41922787Cryo-EM Structures Reveal Mechanism and Inhibition of DNA Targeting by a CRISPR-Cas Surveillance Complex
Q27681727Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA
Q27679265Crystal Structure of the Largest Subunit of a Bacterial RNA-guided Immune Complex and Its Role in DNA Target Binding
Q27678215Crystal structure of Cmr2 suggests a nucleotide cyclase-related enzyme in type III CRISPR-Cas systems
Q35044142Crystal structure of Thermobifida fusca Cse1 reveals target DNA binding site
Q27694776Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli
Q36865378Crystallization and preliminary X-ray diffraction analysis of the Cmr2-Cmr3 subcomplex in the CRISPR-Cas RNA-silencing effector complex
Q27678649Csy4 relies on an unusual catalytic dyad to position and cleave CRISPR RNA
Q26783837Current and future prospects for CRISPR-based tools in bacteria
Q37418237DNA Targeting by a Minimal CRISPR RNA-Guided Cascade
Q38454128DNA and RNA interference mechanisms by CRISPR-Cas surveillance complexes
Q57753376DNA replication roadblocks caused by Cascade interference complexes are alleviated by RecG DNA repair helicase
Q36337685DNA targeting by the type I-G and type I-A CRISPR-Cas systems of Pyrococcus furiosus.
Q36502340DOLORS: versatile strategy for internal labeling and domain localization in electron microscopy
Q34038379Dealing with the evolutionary downside of CRISPR immunity: bacteria and beneficial plasmids
Q35142271Degenerate target sites mediate rapid primed CRISPR adaptation.
Q56610090Delivering CRISPR: a review of the challenges and approaches
Q47194252Design rules of synthetic non-coding RNAs in bacteria
Q90640002Detection of spacer precursors formed in vivo during primed CRISPR adaptation
Q40310719Different genome stability proteins underpin primed and naïve adaptation in E. coli CRISPR-Cas immunity.
Q33919648Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes.
Q34536517Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems
Q90484288Endogenous Type I CRISPR-Cas: From Foreign DNA Defense to Prokaryotic Engineering
Q34724892Essential structural and functional roles of the Cmr4 subunit in RNA cleavage by the Cmr CRISPR-Cas complex
Q27680426Evolution of CRISPR RNA recognition and processing by Cas6 endonucleases
Q38121774Exploiting CRISPR/Cas: interference mechanisms and applications.
Q37699568Fitting CRISPR-associated Cas3 into the helicase family tree
Q28072295Friendly Fire: Biological Functions and Consequences of Chromosomal Targeting by CRISPR-Cas Systems
Q33925900Fulfilling the dream of a perfect genome editing tool
Q34314875Function and regulation of clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR associated (Cas) systems.
Q30381756Functional Analysis of Bacteriophage Immunity through a Type I-E CRISPR-Cas System in Vibrio cholerae and Its Application in Bacteriophage Genome Engineering
Q56809227Functions and Applications of RNA-Guided CRISPR-Cas Immune Systems
Q41968713Genetic determinants of PAM-dependent DNA targeting and pre-crRNA processing in Sulfolobus islandicus.
Q59355982Genetics of CRISPR arrays in Salmonella Typhimurium 14028 associated with foreign DNA decay
Q36339269Go hybrid: EM, crystallography, and beyond
Q33791270Haloarcula hispanica CRISPR authenticates PAM of a target sequence to prime discriminative adaptation
Q34655645Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus
Q91330227Harnessing type I CRISPR-Cas systems for genome engineering in human cells
Q41995190High-throughput analysis of type I-E CRISPR/Cas spacer acquisition in E. coli
Q27681687Identification, structural, and biochemical characterization of a group of large Csn2 proteins involved in CRISPR-mediated bacterial immunity
Q34580667In defense of phage: viral suppressors of CRISPR-mediated adaptive immunity in bacteria
Q39094838In vitro assembly and activity of an archaeal CRISPR-Cas type I-A Cascade interference complex.
Q37311829In vitro reconstitution of an Escherichia coli RNA-guided immune system reveals unidirectional, ATP-dependent degradation of DNA target
Q28485452In vivo protein interactions and complex formation in the Pectobacterium atrosepticum subtype I-F CRISPR/Cas System
Q46495756Inactivation of CRISPR-Cas systems by anti-CRISPR proteins in diverse bacterial species.
Q90149917Jump around: transposons in and out of the laboratory
Q48014045Labeling of Membrane Complexes for Electron Microscopy.
Q34476617Live virus-free or die: coupling of antivirus immunity and programmed suicide or dormancy in prokaryotes
Q27324547Mechanism of CRISPR-RNA guided recognition of DNA targets in Escherichia coli
Q27678641Mechanism of Foreign DNA Selection in a Bacterial Adaptive Immune System
Q35131183Mechanism of foreign DNA recognition by a CRISPR RNA-guided surveillance complex from Pseudomonas aeruginosa
Q35853424Mechanism of substrate selection by a highly specific CRISPR endoribonuclease
Q38057517Memory of viral infections by CRISPR-Cas adaptive immune systems: acquisition of new information
Q47687116Mobile Genetic Elements and Evolution of CRISPR-Cas Systems: All the Way There and Back
Q34581342Molecular insights into DNA interference by CRISPR-associated nuclease-helicase Cas3
Q27002658Molecular mechanisms of CRISPR-mediated microbial immunity
Q34287113Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system
Q38667891Multiple nucleic acid cleavage modes in divergent type III CRISPR systems
Q36386966Native tandem and ion mobility mass spectrometry highlight structural and modular similarities in clustered-regularly-interspaced shot-palindromic-repeats (CRISPR)-associated protein complexes from Escherichia coli and Pseudomonas aeruginosa
Q34104619Nature and intensity of selection pressure on CRISPR-associated genes
Q24631042Newer gene editing technologies toward HIV gene therapy
Q27674624Nucleic acid binding surface and dimer interface revealed by CRISPR-associated CasB protein structures
Q64056373Origins and evolution of CRISPR-Cas systems
Q40416750Overview of CRISPR-Cas9 Biology
Q33635540Pervasive generation of oppositely oriented spacers during CRISPR adaptation
Q35851888Phylogenetic Distribution of CRISPR-Cas Systems in Antibiotic-Resistant Pseudomonas aeruginosa
Q52337923Primed CRISPR adaptation in Escherichia coli cells does not depend on conformational changes in the Cascade effector complex detected in Vitro.
Q92921089Primed adaptation tolerates extensive structural and size variations of the CRISPR RNA guide in Haloarcula hispanica
Q37079815Programmable plasmid interference by the CRISPR-Cas system in Thermococcus kodakarensis
Q37038083Protein domain mapping by internal labeling and single particle electron microscopy
Q41628220Purification, crystallization, crystallographic analysis and phasing of the CRISPR-associated protein Csm2 from Thermotoga maritima
Q28649354RNA targeting by the type III-A CRISPR-Cas Csm complex of Thermus thermophilus
Q89659346RNA-Targeting CRISPR-Cas Systems and Their Applications
Q39135511RNA-based recognition and targeting: sowing the seeds of specificity
Q29614421RNA-guided genetic silencing systems in bacteria and archaea
Q34464381Rational design of a split-Cas9 enzyme complex
Q41773510RcsB-BglJ-mediated activation of Cascade operon does not induce the maturation of CRISPR RNAs in E. coli K12
Q37680429RecA-dependent programmable endonuclease Ref cleaves DNA in two distinct steps
Q27676664Recognition and Cleavage of a Nonstructured CRISPR RNA by Its Processing Endoribonuclease Cas6
Q29615784Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression
Q96609271Repurposing type I-F CRISPR-Cas system as a transcriptional activation tool in human cells
Q93065108Reversible Gene Expression Control in Yersinia pestis by Using an Optimized CRISPR Interference System
Q58787995Role of nucleotide identity in effective CRISPR target escape mutations
Q26998827Small RNAs for defence and regulation in archaea
Q33557501Spacer-length DNA intermediates are associated with Cas1 in cells undergoing primed CRISPR adaptation
Q37518596Staphylococcus epidermidis Csm1 is a 3'-5' exonuclease
Q36675308Streptococcus thermophilus CRISPR-Cas9 Systems Enable Specific Editing of the Human Genome
Q34860823Structural Principles of CRISPR RNA Processing
Q42595828Structural analyses of the CRISPR protein Csc2 reveal the RNA-binding interface of the type I-D Cas7 family
Q38753545Structural and dynamic views of the CRISPR-Cas system at the single-molecule level
Q88007385Structural and functional analyses reveal the contributions of the C- and N-lobes of Argonaute protein to selectivity of RNA target cleavage
Q27703134Structural basis for dimer formation of the CRISPR-associated protein Csm2 of Thermotoga maritima
Q27703998Structural basis for promiscuous PAM recognition in type I-E Cascade from E. coli
Q52423161Structural biology. Cascading into focus.
Q35592632Structural biology. Crystal structure of a CRISPR RNA-guided surveillance complex bound to a ssDNA target
Q34300894Structural biology. Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli
Q36092029Structural biology. Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning
Q64230158Structural organization of a Type III-A CRISPR effector subcomplex determined by X-ray crystallography and cryo-EM
Q28074726Structural studies of RNA-protein complexes: A hybrid approach involving hydrodynamics, scattering, and computational methods
Q50914568Structure Basis for Directional R-loop Formation and Substrate Handover Mechanisms in Type I CRISPR-Cas System.
Q28818412Structure Principles of CRISPR-Cas Surveillance and Effector Complexes
Q27680458Structure and RNA-binding properties of the Type III-A CRISPR-associated protein Csm3
Q33131259Structure and activity of the RNA-targeting Type III-B CRISPR-Cas complex of Thermus thermophilus
Q57753715Structure basis for RNA-guided DNA degradation by Cascade and Cas3
Q37394423Structure of an RNA silencing complex of the CRISPR-Cas immune system.
Q41980729Structure of the CRISPR interference complex CSM reveals key similarities with cascade
Q27677966Structure of the Cmr2 Subunit of the CRISPR-Cas RNA Silencing Complex
Q27676268Structure of the Cmr2-Cmr3 Subcomplex of the Cmr RNA Silencing Complex
Q27679055Structure of the archaeal Cascade subunit Csa5
Q34433997Structures of CRISPR Cas3 offer mechanistic insights into Cascade-activated DNA unwinding and degradation
Q27681624Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation
Q41461432Substrate generation for endonucleases of CRISPR/cas systems
Q36912488Substrate-specific structural rearrangements of human Dicer
Q28079111Survival and Evolution of CRISPR-Cas System in Prokaryotes and Its Applications
Q92387375Systematic analysis of Type I-E Escherichia coli CRISPR-Cas PAM sequences ability to promote interference and primed adaptation
Q33601462Target DNA recognition and cleavage by a reconstituted Type I-G CRISPR-Cas immune effector complex.
Q34430749Target RNA capture and cleavage by the Cmr type III-B CRISPR-Cas effector complex
Q36838370Targeted Transcriptional Repression in Bacteria Using CRISPR Interference (CRISPRi)
Q90243560Targeted transcriptional modulation with type I CRISPR-Cas systems in human cells
Q51148515The Biology of CRISPR-Cas: Backward and Forward.
Q37228823The CRISPR RNA-guided surveillance complex in Escherichia coli accommodates extended RNA spacers
Q36706289The CRISPR-associated gene cas2 of Legionella pneumophila is required for intracellular infection of amoebae
Q38164362The basic building blocks and evolution of CRISPR-CAS systems
Q36114064The box C/D sRNP dimeric architecture is conserved across domain Archaea
Q28080243The diverse and expanding role of mass spectrometry in structural and molecular biology
Q37983037The mysterious RAMP proteins and their roles in small RNA-based immunity
Q52323301The nuts and bolts of the Haloferax CRISPR-Cas system I-B.
Q34044491The role of Cas8 in type I CRISPR interference.
Q35561686The structural biology of CRISPR-Cas systems
Q34337685The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems
Q47692395The unsung heroes of CRISPR.
Q35621447Three CRISPR-Cas immune effector complexes coexist in Pyrococcus furiosus.
Q92686415Transposon-encoded CRISPR-Cas systems direct RNA-guided DNA integration
Q41265458Two distinct DNA binding modes guide dual roles of a CRISPR-Cas protein complex.
Q34988030Type I-E CRISPR-cas systems discriminate target from non-target DNA through base pairing-independent PAM recognition
Q34041195Unravelling the structural and mechanistic basis of CRISPR-Cas systems
Q38332906Unzippers, resolvers and sensors: a structural and functional biochemistry tale of RNA helicases
Q36454957Viral diversity threshold for adaptive immunity in prokaryotes
Q36206656X-ray structure determination using low-resolution electron microscopy maps for molecular replacement
Q42544865crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilus

Search more.