Transposase makes critical contacts with, and is stimulated by, single-stranded DNA at the P element termini in vitro

scientific article

Transposase makes critical contacts with, and is stimulated by, single-stranded DNA at the P element termini in vitro is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1093/EMBOJ/17.7.2122
P932PMC publication ID1170556
P698PubMed publication ID9524133
P5875ResearchGate publication ID51317702

P2093author name stringD C Rio
E L Beall
P2860cites workHuman DNA helicase II: a novel DNA unwinding enzyme identified as the Ku autoantigenQ24336453
A general two-metal-ion mechanism for catalytic RNAQ24562157
Crystal structure at 1.7 A of the bovine papillomavirus-1 E2 DNA-binding domain bound to its DNA targetQ27642089
High-resolution structure of the catalytic domain of avian sarcoma virus integraseQ27729647
Structure of the bacteriophage Mu transposase core: a common structural motif for DNA transposition and retroviral integrationQ27729788
Crystal structure of the catalytic domain of HIV-1 integrase: similarity to other polynucleotidyl transferasesQ27730298
The catalytic domain of avian sarcoma virus integrase: conformation of the active-site residues in the presence of divalent cationsQ27733364
Ku80: product of the XRCC5 gene and its role in DNA repair and V(D)J recombinationQ28115740
Ku70-deficient embryonic stem cells have increased ionizing radiosensitivity, defective DNA end-binding activity, and inability to support V(D)J recombinationQ28243620
DNA-dependent ATPase from HeLa cells is related to human Ku autoantigenQ28244916
DNA-dependent kinase (p350) as a candidate gene for the murine SCID defectQ28307262
Ribozymes: A Distinct Class of MetalloenzymesQ28315324
The chemistry of self-splicing RNA and RNA enzymesQ30457285
Identification and purification of a Drosophila protein that binds to the terminal 31-base-pair inverted repeats of the P transposable elementQ33677309
Reprogramming the purine nucleotide cofactor requirement of Drosophila P element transposase in vivoQ33887134
Identification of discrete functional domains of HIV-1 integrase and their organization within an active multimeric complexQ34057841
Unity in transposition reactionsQ34296425
Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation.Q34320792
The mechanism of transposition of Tc3 in C. elegans.Q34325150
Missing contact probing of DNA-protein interactionsQ34348475
Retroviral integrases and their cousinsQ34387887
Complementation of the ionizing radiation sensitivity, DNA end binding, and V(D)J recombination defects of double-strand break repair mutants by the p86 Ku autoantigenQ34746959
Drosophila P-element transposase is a novel site-specific endonucleaseQ35188701
Transpositional recombination: mechanistic insights from studies of mu and other elementsQ35231355
Polynucleotidyl transfer reactions in transpositional DNA recombinationQ35339797
Identification of residues in the Mu transposase essential for catalysisQ35589106
A stable complex between integrase and viral DNA ends mediates human immunodeficiency virus integration in vitroQ35628704
Analysis of the mechanism of interaction of simian Ku protein with DNAQ35788212
Distinct DNA sequence and structure requirements for the two steps of V(D)J recombination signal cleavageQ35852678
Zinc folds the N-terminal domain of HIV-1 integrase, promotes multimerization, and enhances catalytic activityQ35922636
A Drosophila protein homologous to the human p70 Ku autoimmune antigen interacts with the P transposable element inverted repeatsQ35997819
Contacts between Escherichia coli RNA polymerase and an early promoter of phage T7.Q36350474
Ku86 defines the genetic defect and restores X-ray resistance and V(D)J recombination to complementation group 5 hamster cell mutantsQ36558047
The Drosophila P-element KP repressor protein dimerizes and interacts with multiple sites on P-element DNA.Q36562808
DNA sequence and structure requirements for cleavage of V(D)J recombination signal sequencesQ36562940
Juxtaposition of two viral DNA ends in a bimolecular disintegration reaction mediated by multimers of human immunodeficiency virus type 1 or murine leukemia virus integrase.Q36637758
Residues critical for retroviral integrative recombination in a region that is highly conserved among retroviral/retrotransposon integrases and bacterial insertion sequence transposasesQ36815395
Integration of human immunodeficiency virus DNA: adduct interference analysis of required DNA sitesQ36955625
Excision of Tn10 from the donor site during transposition occurs by flush double-strand cleavages at the transposon terminiQ37021318
DNase protection analysis of the stable synaptic complexes involved in Mu transpositionQ37605009
The phage Mu transpososome core: DNA requirements for assembly and functionQ37623561
Substrate features important for recognition and catalysis by human immunodeficiency virus type 1 integrase identified by using novel DNA substratesQ38307527
Positional information within the Mu transposase tetramer: catalytic contributions of individual monomersQ38358053
Transposase is the only nematode protein required for in vitro transposition of Tc1.Q38360508
What to do at an end: DNA double-strand-break repairQ40372175
The retroviral enzymesQ40394916
Tc1 transposase of Caenorhabditis elegans is an endonuclease with a bipartite DNA binding domainQ40794335
Complementation between HIV integrase proteins mutated in different domainsQ40873688
The Tn7 transposase is a heteromeric complex in which DNA breakage and joining activities are distributed between different gene products.Q41078625
Flexibility of the DNA enhances promoter affinity of Escherichia coli RNA polymeraseQ41082156
The recognition of DNA damageQ41099842
Target site selection in transpositionQ41550231
Impairment of V(D)J recombination in double-strand break repair mutantsQ41560292
P element transposition in vitro proceeds by a cut-and-paste mechanism and uses GTP as a cofactor.Q41629918
Tn7 transposition in vitro proceeds through an excised transposon intermediate generated by staggered breaks in DNA.Q41765684
Assembly of recombinant TFIID reveals differential coactivator requirements for distinct transcriptional activatorsQ42491732
Protected P-element termini suggest a role for inverted-repeat-binding protein in transposase-induced gap repair in Drosophila melanogaster.Q42965450
Cooperative binding of lambda repressors to sites separated by integral turns of the DNA helixQ43484354
Extra sequences found at P element excision sites in Drosophila melanogasterQ43960260
Inversion of the phosphate chirality at the target site of Mu DNA strand transfer: Evidence for a one-step transesterification mechanismQ45829846
Reversal of Integration and DNA Splicing Mediated by Integrase of Human Immunodeficiency VirusQ45857942
Specific recognition nucleotides and their DNA context determine the affinity of E2 protein for 17 binding sites in the BPV-1 genomeQ46082446
Drosophila IRBP/Ku p70 corresponds to the mutagen-sensitive mus309 gene and is involved in P-element excision in vivoQ46097276
cis-acting DNA sequence requirements for P-element transpositionQ46461617
18 DNA contacts probed by modification protection and interference studiesQ46516796
Disruption of the terminal base pairs of retroviral DNA during integrationQ46848322
Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genomeQ48397128
High-frequency P element loss in Drosophila is homolog dependent.Q52449709
Drosophila P element transposase recognizes internal P element DNA sequences.Q52453499
DNA-protein cooperativity in the assembly and stabilization of mu strand transfer complex. Relevance of DNA phasing and att site cleavage.Q54633478
Transposition of Mu DNA: joining of Mu to target DNA can be uncoupled from cleavage at the ends of Mu.Q54758987
Multiple DNA processing reactions underlie Tn7 transpositionQ64389413
Ku polypeptides synthesized in vitro assemble into complexes which recognize ends of double-stranded DNAQ67728079
Efficient Mu transposition requires interaction of transposase with a DNA sequence at the Mu operator: implications for regulationQ69353019
Mechanism of interaction between Ku protein and DNAQ70144316
The three chemical steps of Tn10/IS10 transposition involve repeated utilization of a single active siteQ70908577
The interwoven architecture of the Mu transposase couples DNA synapsis to catalysisQ71032186
An essential interaction between distinct domains of HIV-1 integrase mediates assembly of the active multimerQ72536442
P433issue7
P407language of work or nameEnglishQ1860
P304page(s)2122-2136
P577publication date1998-04-01
P1433published inThe EMBO JournalQ1278554
P1476titleTransposase makes critical contacts with, and is stimulated by, single-stranded DNA at the P element termini in vitro
P478volume17

Reverse relations

cites work (P2860)
Q24540071A highly conserved domain of the maize activator transposase is involved in dimerization
Q39728295An efficient and accurate integration of mini-Mu transposons in vitro: a general methodology for functional genetic analysis and molecular biology applications.
Q46506838Analysis of P element transposase protein-DNA interactions during the early stages of transposition
Q36665807Coincidence of P-insertion sites and breakpoints of deletions induced by activating P elements in Drosophila
Q45085376Construction of gene-targeting vectors: a rapid Mu in vitro DNA transposition-based strategy generating null, potentially hypomorphic, and conditional alleles.
Q33889160DNA binding by the KP repressor protein inhibits P-element transposase activity in vitro
Q34610158Distinct P-element excision products in somatic and germline cells of Drosophila melanogaster.
Q37469272Drosophila IRBP bZIP heterodimer binds P-element DNA and affects hybrid dysgenesis
Q34631159Germline transformants spreading out to many insect species
Q33855382Guanosine triphosphate acts as a cofactor to promote assembly of initial P-element transposase-DNA synaptic complexes
Q34570772Lig4 and rad54 are required for repair of DNA double-strand breaks induced by P-element excision in Drosophila
Q38536147Mechanisms of DNA Transposition
Q35446814P transposable elements in Drosophila and other eukaryotic organisms
Q90975856Structure of a P element transposase-DNA complex reveals unusual DNA structures and GTP-DNA contacts
Q42063887Testing the palindromic target site model for DNA transposon insertion using the Drosophila melanogaster P-element
Q38350168The C-terminus of the Hermes transposase contains a protein multimerization domain
Q41995829The dynamic Mu transpososome: MuB activation prevents disintegration
Q35801161Transposable Phage Mu
Q37485954True reversal of Mu integration
Q34162471Whole genome resequencing reveals natural target site preferences of transposable elements in Drosophila melanogaster

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