Akt2 phosphorylates ezrin to trigger NHE3 translocation and activation

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Akt2 phosphorylates ezrin to trigger NHE3 translocation and activation is …
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scholarly articleQ13442814

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P356DOI10.1074/JBC.M409471200
P932PMC publication ID1237052
P698PubMed publication ID15531580

P50authorEugene B ChangQ88434885
P2093author name stringMark W Musch
Jerrold R Turner
Yingmin Wang
Harn Shiue
P2860cites workInsulin-stimulated GLUT4 translocation requires the CAP-dependent activation of TC10Q24291135
Mechanism of activation of protein kinase B by insulin and IGF-1Q24309528
NHE3 kinase A regulatory protein E3KARP binds the epithelial brush border Na+/H+ exchanger NHE3 and the cytoskeletal protein ezrinQ24315976
The EBP50-moesin interaction involves a binding site regulated by direct masking on the FERM domainQ24316456
Ezrin self-association involves binding of an N-terminal domain to a normally masked C-terminal domain that includes the F-actin binding siteQ24617964
Ezrin, a plasma membrane-microfilament linker, signals cell survival through the phosphatidylinositol 3-kinase/Akt pathwayQ24648522
Lipid Raft targeting of the TC10 amino terminal domain is responsible for disruption of adipocyte cortical actinQ24669996
Scansite 2.0: Proteome-wide prediction of cell signaling interactions using short sequence motifsQ24671990
Phosphoinositide binding and phosphorylation act sequentially in the activation mechanism of ezrinQ24676944
Rho-kinase phosphorylates COOH-terminal threonines of ezrin/radixin/moesin (ERM) proteins and regulates their head-to-tail associationQ24678197
Morphogenic effects of ezrin require a phosphorylation-induced transition from oligomers to monomers at the plasma membraneQ24683879
Structure of the ERM protein moesin reveals the FERM domain fold masked by an extended actin binding tail domainQ27622801
The exocyst complex is required for targeting of Glut4 to the plasma membrane by insulinQ28190100
Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeletonQ28202663
Cyclic AMP-dependent protein kinase phosphorylates merlin at serine 518 independently of p21-activated kinase and promotes merlin-ezrin heterodimerizationQ28246519
Na+-H+ exchanger 3 (NHE3) is present in lipid rafts in the rabbit ileal brush border: a role for rafts in trafficking and rapid stimulation of NHE3Q28365699
Ezrin is a downstream effector of trafficking PKC-integrin complexes involved in the control of cell motilityQ28366134
Physiological role of Akt in insulin-stimulated translocation of GLUT4 in transfected rat adipose cellsQ28586458
NHERF-1 uniquely transduces the cAMP signals that inhibit sodium-hydrogen exchange in mouse renal apical membranesQ28586939
A specific inhibitor of phosphatidylinositol 3-kinase, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY294002)Q28640016
ERM proteins and merlin: integrators at the cell cortexQ29619893
Carboxy-terminal vesicular stomatitis virus G protein-tagged intestinal Na+-dependent glucose cotransporter (SGLT1): maintenance of surface expression and global transport function with selective perturbation of transport kinetics and polarized exprQ30423828
Lipid raft microdomain compartmentalization of TC10 is required for insulin signaling and GLUT4 translocationQ30441963
Toward a PKB inhibitor: modification of a selective PKA inhibitor by rational designQ30840946
Use of RNA interference-mediated gene silencing and adenoviral overexpression to elucidate the roles of AKT/protein kinase B isoforms in insulin actionsQ30925087
The lateral mobility of NHE3 on the apical membrane of renal epithelial OK cells is limited by the PDZ domain proteins NHERF1/2, but is dependent on an intact actin cytoskeleton as determined by FRAP.Q33204357
Activation of the pro-survival phosphatidylinositol 3-kinase/AKT pathway by transforming growth factor-beta1 in mesenchymal cells is mediated by p38 MAPK-dependent induction of an autocrine growth factor.Q34333813
ERM proteins and NF2 tumor suppressor: the Yin and Yang of cortical actin organization and cell growth signalingQ34495193
Ezrin regulates NHE3 translocation and activation after Na+-glucose cotransport.Q35322212
Association of the myosin-binding subunit of myosin phosphatase and moesin: dual regulation of moesin phosphorylation by Rho-associated kinase and myosin phosphataseQ36289185
RhoA-dependent phosphorylation and relocalization of ERM proteins into apical membrane/actin protrusions in fibroblastsQ38610718
Ezrin function is required for ROCK-mediated fibroblast transformation by the Net and Dbl oncogenesQ40370592
Akt-dependent phosphorylation negatively regulates the transcriptional activity of dHAND by inhibiting the DNA binding activityQ40529059
Runx2 induces osteoblast and chondrocyte differentiation and enhances their migration by coupling with PI3K-Akt signalingQ40540364
The NHE1 Na+/H+ exchanger recruits ezrin/radixin/moesin proteins to regulate Akt-dependent cell survival.Q40564766
Inhibition and redistribution of NHE3, the apical Na+/H+ exchanger, by Clostridium difficile toxin B.Q40567906
Akt kinase activation blocks apoptosis in intestinal epithelial cells by inhibiting caspase-3 after polyamine depletionQ40578104
Ca2+-dependent inhibition of NHE3 requires PKC alpha which binds to E3KARP to decrease surface NHE3 containing plasma membrane complexesQ40637935
Lysophosphatidic acid stimulates brush border Na+/H+ exchanger 3 (NHE3) activity by increasing its exocytosis by an NHE3 kinase A regulatory protein-dependent mechanismQ40669337
Moesin functions antagonistically to the Rho pathway to maintain epithelial integrityQ40679590
Differential effects of phosphatidylinositol 3-kinase inhibition on intracellular signals regulating GLUT4 translocation and glucose transportQ40775117
Ezrin binding domain-deficient NHERF attenuates cAMP-mediated inhibition of Na(+)/H(+) exchange in OK cellsQ40791959
Phosphorylation of myosin-binding subunit (MBS) of myosin phosphatase by Rho-kinase in vivoQ40915136
The role of NHERF and E3KARP in the cAMP-mediated inhibition of NHE3.Q40999069
Phosphoinositide 3-kinase is required for insulin-induced but not for growth hormone- or hyperosmolarity-induced glucose uptake in 3T3-L1 adipocytesQ42443687
Akt2, phosphatidylinositol 3-kinase, and PTEN are in lipid rafts of intestinal cells: role in absorption and differentiationQ42454922
An inhibitor of p38 mitogen-activated protein kinase prevents insulin-stimulated glucose transport but not glucose transporter translocation in 3T3-L1 adipocytes and L6 myotubesQ42470059
Constitutively active phosphatidylinositol 3-kinase and AKT are sufficient to stimulate the epithelial Na+/H+ exchanger 3.Q42504933
Ezrin has a COOH-terminal actin-binding site that is conserved in the ezrin protein familyQ42720142
Activation of ERM proteins in vivo by Rho involves phosphatidyl-inositol 4-phosphate 5-kinase and not ROCK kinasesQ42809055
Rho-dependent and -independent activation mechanisms of ezrin/radixin/moesin proteins: an essential role for polyphosphoinositides in vivo.Q42814440
Protein kinase B/AKT 1 plays a pivotal role in insulin-like growth factor-1 receptor signaling induced 3T3-L1 adipocyte differentiationQ42828148
NHE3-dependent cytoplasmic alkalinization is triggered by Na(+)-glucose cotransport in intestinal epitheliaQ43766328
A dileucine motif targets the sulfate anion transporter sat-1 to the basolateral membrane in renal cell linesQ44834860
Requirement of the epidermal growth factor receptor in renal epithelial cell proliferation and migrationQ47303356
Role of p38 MAPK and MAPKAPK-2 in angiotensin II-induced Akt activation in vascular smooth muscle cellsQ47764569
Renal and intestinal absorptive defects in mice lacking the NHE3 Na+/H+ exchangerQ47852356
Soluble ezrin purified from placenta exists as stable monomers and elongated dimers with masked C-terminal ezrin-radixin-moesin association domainsQ70801487
The apical Na(+)/H(+) exchanger isoform NHE3 is regulated by the actin cytoskeletonQ73066614
RhoA and rho kinase regulate the epithelial Na+/H+ exchanger NHE3. Role of myosin light chain phosphorylationQ73992917
Protein kinase C-theta phosphorylation of moesin in the actin-binding sequenceQ74358920
Cutting edge: negative regulation of immune synapse formation by anchoring lipid raft to cytoskeleton through Cbp-EBP50-ERM assemblyQ77452189
P4510describes a project that usesImageJQ1659584
P433issue2
P407language of work or nameEnglishQ1860
P921main subjectphosphorylationQ242736
P304page(s)1688-1695
P577publication date2004-11-04
P1433published inJournal of Biological ChemistryQ867727
P1476titleAkt2 phosphorylates ezrin to trigger NHE3 translocation and activation
P478volume280

Reverse relations

cites work (P2860)
Q37608417AKT and GSK-3 are necessary for direct ezrin binding to NHE3 as part of a C-terminal stimulatory complex: role of a novel Ser-rich NHE3 C-terminal motif in NHE3 activity and trafficking
Q35104869Akt inhibitor MK-2206 promotes anti-tumor activity and cell death by modulation of AIF and Ezrin in colorectal cancer
Q54638508Anti-apoptotic PI3K/Akt signaling by sodium/glucose transporter 1 reduces epithelial barrier damage and bacterial translocation in intestinal ischemia.
Q36540696Atypical protein kinase C (iota) activates ezrin in the apical domain of intestinal epithelial cells
Q36698786Beyond Ussing's chambers: contemporary thoughts on integration of transepithelial transport
Q57107234Bile acids inhibit Na⁺/H⁺ exchanger and Cl⁻/HCO₃⁻ exchanger activities via cellular energy breakdown and Ca²⁺ overload in human colonic crypts
Q36805316Comparative study of ezrin phosphorylation among different tissues: more is good; too much is bad.
Q89578153Contributions of Myosin Light Chain Kinase to Regulation of Epithelial Paracellular Permeability and Mucosal Homeostasis
Q36704497Coupling between apical and paracellular transport processes
Q42586814D-Glucose Acts via Sodium/Glucose Cotransporter 1 to Increase NHE3 in Mouse Jejunal Brush Border by a Na+/H+ Exchange Regulatory Factor 2–Dependent Process
Q40738109Diet induced obesity in rats reduces NHE3 and Na(+) /K(+) -ATPase expression in the kidney.
Q24322564EGF receptor transactivation and PI3-kinase mediate stimulation of ERK by alpha(2A)-adrenoreceptor in intestinal epithelial cells: a role in wound healing
Q47938284ERM protein moesin is phosphorylated by advanced glycation end products and modulates endothelial permeability
Q33698044Epigenetic drugs can stimulate metastasis through enhanced expression of the pro-metastatic Ezrin gene
Q33782512Epithelial myosin light chain kinase activation induces mucosal interleukin-13 expression to alter tight junction ion selectivity
Q37682332Ezrin expression and cell survival regulation in colorectal cancer
Q28251491Ezrin induces long-range interdomain allostery in the scaffolding protein NHERF1
Q36369093Ezrin interacts with S100A4 via both its N- and C-terminal domains.
Q50697750Ezrin/moesin in motile Walker 256 carcinosarcoma cells: signal-dependent relocalization and role in migration.
Q36320446Fluid shear triggers microvilli formation via mechanosensitive activation of TRPV6
Q42848424Functional role of glucose metabolism, osmotic stress, and sodium-glucose cotransporter isoform-mediated transport on Na+/H+ exchanger isoform 3 activity in the renal proximal tubule
Q30497357Glucose-induced ERM protein activation and translocation regulates insulin secretion
Q46102053High turnover of ezrin T567 phosphorylation: conformation, activity, and cellular function
Q40131520Hsp27 regulates Akt activation and polymorphonuclear leukocyte apoptosis by scaffolding MK2 to Akt signal complex
Q34985526Insulin resistance, obesity, hypertension, and renal sodium transport
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Q37510693Involvement of Akt in neurite outgrowth
Q39717269Knockdown of moesin expression accelerates cellular senescence of human dermal microvascular endothelial cells.
Q46601631Lumenal adenosine and AMP rapidly increase glucose transport by intact small intestine
Q40263729MAPKAPK-2 is a critical signaling intermediate in NHE3 activation following Na+-glucose cotransport.
Q57364667Mechanisms of Ion Transport Regulation by Microfilaments
Q35569905Molecular basis of epithelial barrier regulation: from basic mechanisms to clinical application
Q85707925Molecular transport machinery involved in orchestrating luminal acid-induced duodenal bicarbonate secretion in vivo
Q37485479NHE3 regulatory complexes
Q64076170Neutrophil Cell Shape Change: Mechanism and Signalling during Cell Spreading and Phagocytosis
Q38958544Phosphorylation of AKT induced by phosphorylated Hsp27 confers the apoptosis-resistance in t-AUCB-treated glioblastoma cells in vitro
Q38720389Plasticity of the brush border - the yin and yang of intestinal homeostasis
Q36848529Rapamycin Inhibition of mTOR Reduces Levels of the Na+/H+ Exchanger 3 in Intestines of Mice and Humans, Leading to Diarrhea
Q37428401Regulation of expression of microvillus membrane proteins by estrogen in baboon fetal ovarian oocytes
Q38141920Regulation of paracellular permeability: factors and mechanisms.
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Q52337636TGFβ and IGF1R signaling activate protein kinase A through differential regulation of ezrin phosphorylation in colon cancer cells.
Q34720146Telencephalin protects PAJU cells from amyloid beta protein-induced apoptosis by activating the ezrin/radixin/moesin protein family/phosphatidylinositol-3-kinase/protein kinase B pathway
Q35555968The ERM protein, ezrin, regulates neutrophil transmigration by modulating the apical localization of MRP2 in response to the SipA effector protein during Salmonella Typhimurium infection
Q37297700The calcineurin homologous protein-1 increases Na(+)/H(+) -exchanger 3 trafficking via ezrin phosphorylation
Q30572906WNK1-OSR1 kinase-mediated phospho-activation of Na+-K+-2Cl- cotransporter facilitates glioma migration

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