Conserved Arginines at the P-Protein Stalk Binding Site and the Active Site Are Critical for Ribosome Interactions of Shiga Toxins but Do Not Contribute to Differences in the Affinity of the A1 Subunits for the Ribosome

scientific article published on 06 September 2016

Conserved Arginines at the P-Protein Stalk Binding Site and the Active Site Are Critical for Ribosome Interactions of Shiga Toxins but Do Not Contribute to Differences in the Affinity of the A1 Subunits for the Ribosome is …
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scholarly articleQ13442814

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P356DOI10.1128/IAI.00630-16
P8608Fatcat IDrelease_e2ad4gz2xjchbjprebd454wij4
P932PMC publication ID5116708
P698PubMed publication ID27600507

P2093author name stringDebaleena Basu
Nilgun E Tumer
Jennifer N Kahn
Xiao-Ping Li
P2860cites workThe C-terminal fragment of the ribosomal P protein complexed to trichosanthin reveals the interaction between the ribosome-inactivating protein and the ribosomeQ24642956
Toxin gene expression by shiga toxin-producing Escherichia coli: the role of antibiotics and the bacterial SOS responseQ24647531
Evidence that glutamic acid 167 is an active-site residue of Shiga-like toxin IQ24651553
Transition state analogues in structures of ricin and saporin ribosome-inactivating proteinsQ27658223
Structure of EF-G-ribosome complex in a pretranslocation stateQ27685333
A new system for naming ribosomal proteinsQ28307640
Shiga-toxin-producing Escherichia coli and haemolytic uraemic syndromeQ33365488
Hemolytic uremic syndrome; pathogenesis, treatment, and outcomeQ33365631
The A1 Subunit of Shiga Toxin 2 Has Higher Affinity for Ribosomes and Higher Catalytic Activity than the A1 Subunit of Shiga Toxin 1.Q33426867
Associations between virulence factors of Shiga toxin-producing Escherichia coli and disease in humansQ33505189
Development of a quantitative RT-PCR assay to examine the kinetics of ribosome depurination by ribosome inactivating proteins using Saccharomyces cerevisiae as a modelQ33753680
Functional divergence between the two P1-P2 stalk dimers on the ribosome in their interaction with ricin A chainQ33909090
Site of action of a Vero toxin (VT2) from Escherichia coli O157:H7 and of Shiga toxin on eukaryotic ribosomes. RNA N-glycosidase activity of the toxins.Q34049372
Different classes of antibiotics differentially influence shiga toxin productionQ34108726
Charged and hydrophobic surfaces on the a chain of shiga-like toxin 1 recognize the C-terminal domain of ribosomal stalk proteinsQ34166278
Shiga toxinsQ34298388
Pentameric organization of the ribosomal stalk accelerates recruitment of ricin a chain to the ribosome for depurinationQ34438778
Identification of amino acids critical for the cytotoxicity of Shiga toxin 1 and 2 in Saccharomyces cerevisiaeQ34663367
The puzzling lateral flexible stalk of the ribosomeQ35179486
Shiga toxin 1 is more dependent on the P proteins of the ribosomal stalk for depurination activity than Shiga toxin 2Q35501790
Functional role of the sarcin-ricin loop of the 23S rRNA in the elongation cycle of protein synthesisQ35946837
Delivery into cells: lessons learned from plant and bacterial toxinsQ36091291
N-glycosylation does not affect the catalytic activity of ricin a chain but stimulates cytotoxicity by promoting its transport out of the endoplasmic reticulumQ36301063
The ribosomal stalk is required for ribosome binding, depurination of the rRNA and cytotoxicity of ricin A chain in Saccharomyces cerevisiaeQ37088333
A two-step binding model proposed for the electrostatic interactions of ricin a chain with ribosomes.Q37180842
Arginine residues on the opposite side of the active site stimulate the catalysis of ribosome depurination by ricin A chain by interacting with the P-protein stalkQ37234010
Structures of eukaryotic ribosomal stalk proteins and its complex with trichosanthin, and their implications in recruiting ribosome-inactivating proteins to the ribosomesQ38365820
Role of arginine 180 and glutamic acid 177 of ricin toxin A chain in enzymatic inactivation of ribosomesQ40647546
Role of glutamic acid 177 of the ricin toxin A chain in enzymatic inactivation of ribosomesQ40651036
Structural basis for the function of the ribosomal L7/12 stalk in factor binding and GTPase activationQ41627064
Interaction between trichosanthin, a ribosome-inactivating protein, and the ribosomal stalk protein P2 by chemical shift perturbation and mutagenesis analysesQ41934174
The catalytic subunit of shiga-like toxin 1 interacts with ribosomal stalk proteins and is inhibited by their conserved C-terminal domainQ42648942
Atomic mutagenesis reveals A2660 of 23S ribosomal RNA as key to EF-G GTPase activationQ43116255
Mutations affecting the activity of the Shiga-like toxin I A-chainQ44645523
Cryo-EM visualization of a viral internal ribosome entry site bound to human ribosomes: the IRES functions as an RNA-based translation factor.Q54501268
Construction of mutant genes for a non-toxic verotoxin 2 variant (VT2vp1) of Escherichia coli and characterization of purified mutant toxins.Q54645159
Analysis of several key active site residues of ricin A chain by mutagenesis and X-ray crystallographyQ67471387
Site-directed mutagenesis of ricin A-chain and implications for the mechanism of actionQ67959668
Yeast ribosomal P0 protein has two separate binding sites for P1/P2 proteinsQ83013729
An improved method for whole protein extraction from yeast Saccharomyces cerevisiaeQ85042457
P433issue12
P407language of work or nameEnglishQ1860
P304page(s)3290-3301
P577publication date2016-09-06
P1433published inInfection and ImmunityQ6029193
P1476titleConserved Arginines at the P-Protein Stalk Binding Site and the Active Site Are Critical for Ribosome Interactions of Shiga Toxins but Do Not Contribute to Differences in the Affinity of the A1 Subunits for the Ribosome
P478volume84

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cites work (P2860)
Q33612042Differences in Ribosome Binding and Sarcin/Ricin Loop Depurination by Shiga and Ricin Holotoxins
Q55515121Functional Assays for Measuring the Catalytic Activity of Ribosome Inactivating Proteins.

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