Identification of amino acids critical for the cytotoxicity of Shiga toxin 1 and 2 in Saccharomyces cerevisiae

scientific article

Identification of amino acids critical for the cytotoxicity of Shiga toxin 1 and 2 in Saccharomyces cerevisiae is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1016/J.TOXICON.2010.12.006
P932PMC publication ID3055938
P698PubMed publication ID21184769
P5875ResearchGate publication ID49708372

P2093author name stringRong Di
Nilgun E Tumer
Peter C Kahn
Varsha Shete
Hemalatha Saidasan
Eric Kyu
P2860cites workDiarrheagenic Escherichia coliQ24533466
Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infectionsQ24550740
Evidence that glutamic acid 167 is an active-site residue of Shiga-like toxin IQ24651553
Buried charged surface in proteinsQ27628399
Crystal structure of the holotoxino from Shigella dysenteriae at 2.5 Å resolutionQ27729840
The interpretation of protein structures: Estimation of static accessibilityQ27860750
Response to Shiga toxin 1 and 2 in a baboon model of hemolytic uremic syndromeQ33346480
Shiga toxin: biochemistry, genetics, mode of action, and role in pathogenesisQ33350981
Hemolytic uremic syndrome; pathogenesis, treatment, and outcomeQ33365631
Hemolytic-uremic syndrome and enterohemorrhagic Escherichia coliQ33492019
Comparison of the relative toxicities of Shiga-like toxins type I and type II for miceQ33605233
Disruption of an internal membrane-spanning region in Shiga toxin 1 reduces cytotoxicityQ33767533
Isolation and characterization of pokeweed antiviral protein mutations in Saccharomyces cerevisiae: identification of residues important for toxicityQ33981471
Site of action of a Vero toxin (VT2) from Escherichia coli O157:H7 and of Shiga toxin on eukaryotic ribosomes. RNA N-glycosidase activity of the toxins.Q34049372
Ricin inhibits activation of the unfolded protein response by preventing splicing of the HAC1 mRNA.Q34510212
Pathways followed by ricin and Shiga toxin into cellsQ34589230
Ribosome depurination is not sufficient for ricin-mediated cell death in Saccharomyces cerevisiaeQ35689052
The electrostatic character of the ribosomal surface enables extraordinarily rapid target location by ribotoxinsQ35741993
Delivery into cells: lessons learned from plant and bacterial toxinsQ36091291
Binding of adenine to Stx2, the protein toxin from Escherichia coli O157:H7.Q36459565
The ribosomal stalk is required for ribosome binding, depurination of the rRNA and cytotoxicity of ricin A chain in Saccharomyces cerevisiaeQ37088333
A two-step binding model proposed for the electrostatic interactions of ricin a chain with ribosomes.Q37180842
Rapid and specific detection of verotoxin genes in Escherichia coli by the polymerase chain reactionQ37228063
Monoclonal antibody 11E10, which neutralizes shiga toxin type 2 (Stx2), recognizes three regions on the Stx2 A subunit, blocks the enzymatic action of the toxin in vitro, and alters the overall cellular distribution of the toxin.Q37256478
Generation of pokeweed antiviral protein mutations in Saccharomyces cerevisiae: evidence that ribosome depurination is not sufficient for cytotoxicity.Q37483544
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A role for the protease-sensitive loop region of Shiga-like toxin 1 in the retrotranslocation of its A1 domain from the endoplasmic reticulum lumenQ38328315
Pathogenesis of shigella diarrhea. XI. Isolation of a shigella toxin-binding glycolipid from rabbit jejunum and HeLa cells and its identification as globotriaosylceramideQ38350915
Shiga toxin 1 induces apoptosis through the endoplasmic reticulum stress response in human monocytic cellsQ40051377
Mechanism of ricin-induced apoptosis in human cervical cancer cells.Q40458522
Role of glutamic acid 177 of the ricin toxin A chain in enzymatic inactivation of ribosomesQ40651036
Evidence for the importance of electrostatics in the function of two distinct families of ribosome inactivating toxinsQ42413458
The catalytic subunit of shiga-like toxin 1 interacts with ribosomal stalk proteins and is inhibited by their conserved C-terminal domainQ42648942
Regulated expression of the Shiga toxin B gene induces apoptosis in mammalian fibroblastic cellsQ42810250
Kinetic analysis of binding between Shiga toxin and receptor glycolipid Gb3Cer by surface plasmon resonanceQ43738152
Pokeweed antiviral protein regulates the stability of its own mRNA by a mechanism that requires depurination but can be separated from depurination of the alpha-sarcin/ricin loop of rRNA.Q44097420
Don't eat the spinach--controlling foodborne infectious diseaseQ44290276
Mutations affecting the activity of the Shiga-like toxin I A-chainQ44645523
Structure of shiga toxin type 2 (Stx2) from Escherichia coli O157:H7.Q44838252
Evidence for retro-translocation of pokeweed antiviral protein from endoplasmic reticulum into cytosol and separation of its activity on ribosomes from its activity on capped RNA.Q45264128
Furin-induced cleavage and activation of Shiga toxinQ49165095
Calculation of molecular volumes and areas for structures of known geometry.Q52678993
Globotriosyl ceramide is specifically recognized by the Escherichia coli verocytotoxin 2.Q54385143
Construction of mutant genes for a non-toxic verotoxin 2 variant (VT2vp1) of Escherichia coli and characterization of purified mutant toxins.Q54645159
The role of tyrosine-114 in the enzymatic activity of the Shiga-like toxin I A-chain.Q54648744
Characterization of non-toxic mutant toxins of Vero toxin 1 that were constructed by replacing amino acids in the A subunit.Q54651945
Glycolipid binding of purified and recombinant Escherichia coli produced verotoxin in vitro.Q54764104
Preparation of VT1 and VT2 hybrid toxins from their purified dissociated subunits. Evidence for B subunit modulation of a subunit functionQ67913265
Site-directed mutagenesis of ricin A-chain and implications for the mechanism of actionQ67959668
Ricin A chain utilises the endoplasmic reticulum-associated protein degradation pathway to enter the cytosol of yeastQ73047414
Modeling and alanine scanning mutagenesis studies of recombinant pokeweed antiviral proteinQ73396552
The RNA-N-glycosidase activity of Shiga-like toxin I: kinetic parameters of the native and activated toxinQ73952496
The C-terminus of pokeweed antiviral protein has distinct roles in transport to the cytosol, ribosome depurination and cytotoxicityQ79740954
P433issue4
P921main subjectcytotoxicityQ246181
Saccharomyces cerevisiaeQ719725
P304page(s)525-539
P577publication date2010-12-22
P1433published inToxiconQ7830412
P1476titleIdentification of amino acids critical for the cytotoxicity of Shiga toxin 1 and 2 in Saccharomyces cerevisiae
P478volume57

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cites work (P2860)
Q34166278Charged and hydrophobic surfaces on the a chain of shiga-like toxin 1 recognize the C-terminal domain of ribosomal stalk proteins
Q37424949Conserved Arginines at the P-Protein Stalk Binding Site and the Active Site Are Critical for Ribosome Interactions of Shiga Toxins but Do Not Contribute to Differences in the Affinity of the A1 Subunits for the Ribosome
Q26858938Do the A subunits contribute to the differences in the toxicity of Shiga toxin 1 and Shiga toxin 2?
Q37977331Induction of apoptosis by ribosome inactivating proteins: importance of N-glycosidase activity
Q33921062Interaction of ricin and Shiga toxins with ribosomes
Q28081661Pokeweed antiviral protein: its cytotoxicity mechanism and applications in plant disease resistance
Q28488900Purification and characterization of Shiga toxin 2f, an immunologically unrelated subtype of Shiga toxin 2
Q27021676Shiga Toxin (Stx) Classification, Structure, and Function
Q35501790Shiga toxin 1 is more dependent on the P proteins of the ribosomal stalk for depurination activity than Shiga toxin 2
Q92153341Structural and Functional Characterization of Stx2k, a New Subtype of Shiga Toxin 2
Q33426867The A1 Subunit of Shiga Toxin 2 Has Higher Affinity for Ribosomes and Higher Catalytic Activity than the A1 Subunit of Shiga Toxin 1.

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