Structural basis of transcription initiation: RNA polymerase holoenzyme at 4 A resolution

scientific article

Structural basis of transcription initiation: RNA polymerase holoenzyme at 4 A resolution is …
instance of (P31):
scholarly articleQ13442814

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P819ADS bibcode2002Sci...296.1280M
P356DOI10.1126/SCIENCE.1069594
P3181OpenCitations bibliographic resource ID715716
P698PubMed publication ID12016306
P5875ResearchGate publication ID11355986

P2093author name stringKatsuhiko S Murakami
Seth A Darst
Shoko Masuda
P433issue5571
P407language of work or nameEnglishQ1860
P304page(s)1280-4
P577publication date2002-05-17
P1433published inScienceQ192864
P1476titleStructural basis of transcription initiation: RNA polymerase holoenzyme at 4 A resolution
P478volume296

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cites work (P2860)
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Q38320906Escherichia coli RNA polymerase contacts outside the -10 promoter element are not essential for promoter melting
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Q41823965In vivo assembly and single-molecule characterization of the transcription machinery from Shewanella oneidensis MR-1.
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Q41555438Initial transcribed region sequences influence the composition and functional properties of the bacterial elongation complex
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Q37272203Insights into the mechanism of initial transcription in Escherichia coli RNA polymerase
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Q54286054Kinetics of promoter escape by bacterial RNA polymerase: effects of promoter contacts and transcription bubble collapse.
Q42068625Lineage-specific amino acid substitutions in region 2 of the RNA polymerase sigma subunit affect the temperature of promoter opening
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Q36228825Mechanism of bacterial transcription initiation: RNA polymerase - promoter binding, isomerization to initiation-competent open complexes, and initiation of RNA synthesis
Q45282565Mechanistic differences in promoter DNA melting by Thermus aquaticus and Escherichia coli RNA polymerases
Q37375642Minimal promoter systems reveal the importance of conserved residues in the B-finger of human transcription factor IIB.
Q37106646Modus operandi of the bacterial RNA polymerase containing the sigma54 promoter-specificity factor
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Q35222379Mutational analysis of sigma70 region 4 needed for appropriation by the bacteriophage T4 transcription factors AsiA and MotA.
Q44259001Mutational and functional analysis of a segment of the sigma family bacteriophage T4 late promoter recognition protein gp55.
Q34406989Mutations enabling displacement of tryptophan by 4-fluorotryptophan as a canonical amino acid of the genetic code
Q35921461Mutations in rpoBC suppress the defects of a Sinorhizobium meliloti relA mutant
Q44195387Mutations of bacterial RNA polymerase leading to resistance to microcin j25.
Q36144874Mycobacterium tuberculosis RbpA protein is a new type of transcriptional activator that stabilizes the σ A-containing RNA polymerase holoenzyme
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Q37021534Nascent RNA length dictates opposing effects of NusA on antitermination
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Q36347844New insights into the mechanism of initial transcription: the T7 RNA polymerase mutant P266L transitions to elongation at longer RNA lengths than wild type
Q38534594Nickel-responsive transcriptional regulators
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Q33934871One-step DNA melting in the RNA polymerase cleft opens the initiation bubble to form an unstable open complex
Q24633938Opening and closing of the bacterial RNA polymerase clamp
Q34875804Organization of an activator-bound RNA polymerase holoenzyme
Q30368988Overview of protein structural and functional folds.
Q37124879Phage-encoded inhibitor of Staphylococcus aureus transcription exerts context-dependent effects on promoter function in a modified Escherichia coli-based transcription system
Q40468992Photo-cross-linking of a purified preinitiation complex reveals central roles for the RNA polymerase II mobile clamp and TFIIE in initiation mechanisms.
Q36759096Plant sigma factors and their role in plastid transcription.
Q88795755Possible roles of σ-dependent RNA polymerase pausing in transcription regulation
Q54512741Preparation and characterization of recombinant Thermus aquaticus RNA polymerase.
Q36580540Preparation and preliminary X-ray diffraction analysis of crystals of bacterial flagellar sigma factor sigma 28 in complex with the sigma 28-binding region of its antisigma factor, FlgM
Q41902743Prevalence of RNA polymerase stalling at Escherichia coli promoters after open complex formation
Q44720075Principles and methods of affinity cleavage in studying transcription
Q41514407Probing DNA binding, DNA opening, and assembly of a downstream clamp/jaw in Escherichia coli RNA polymerase-lambdaP(R) promoter complexes using salt and the physiological anion glutamate
Q38346162Probing the role of region 2 of Escherichia coli sigma 70 in nucleation and maintenance of the single-stranded DNA bubble in RNA polymerase-promoter open complexes
Q36594585Promoter activation by repositioning of RNA polymerase
Q45995106Promoter architecture and response to a positive regulator of archaeal transcription.
Q34816592Promoter clearance and escape in prokaryotes
Q34004968Promoter melting triggered by bacterial RNA polymerase occurs in three steps
Q40935619Promoter spacer DNA plays an active role in integrating the functional consequences of RNA polymerase contacts with -10 and -35 promoter elements
Q27675215Promoter-Specific Transcription Inhibition in Staphylococcus aureus by a Phage Protein
Q34001711Properties of Bacillus subtilis sigma A factors with region 1.1 and the conserved Arg-103 at the N terminus of region 1.2 deleted
Q54442568Properties of RNA polymerase bypass mutants: implications for the role of ppGpp and its co-factor DksA in controlling transcription dependent on sigma54.
Q78488186Protein-protein and protein-DNA interactions of sigma70 region 4 involved in transcription activation by lambdacI
Q28484727Proteome organization in a genome-reduced bacterium
Q37273159RNA Polymerase Pausing during Initial Transcription
Q92871511RNA Polymerase: Step-by-Step Kinetics and Mechanism of Transcription Initiation
Q90008722RNA extension drives a stepwise displacement of an initiation-factor structural module in initial transcription
Q35162629RNA polymerase II at initiation
Q38045685RNA polymerase II transcription: structure and mechanism
Q27657756RNA polymerase II-TFIIB structure and mechanism of transcription initiation
Q41628900RNA polymerase II/TFIIF structure and conserved organization of the initiation complex
Q35121887RNA polymerase holoenzyme: structure, function and biological implications
Q40197974RNA polymerase motions during promoter melting.
Q24802311RNA polymerase mutants defective in the initiation of transcription-coupled DNA repair
Q39895370RNA polymerase mutations that impair conversion to a termination-resistant complex by Q antiterminator proteins
Q38121625RNA polymerase: in search of promoters
Q42957000RNA-mediated destabilization of the sigma(70) region 4/beta flap interaction facilitates engagement of RNA polymerase by the Q antiterminator
Q27934170RPAP1, a novel human RNA polymerase II-associated protein affinity purified with recombinant wild-type and mutated polymerase subunits
Q35808746Real-time footprinting of DNA in the first kinetically significant intermediate in open complex formation by Escherichia coli RNA polymerase
Q91975437Recent Advances in Understanding σ70-Dependent Transcription Initiation Mechanisms
Q42002605Region 1.2 of the RNA polymerase sigma subunit controls recognition of the -10 promoter element
Q57469398Region 3.2 of the σ factor controls the stability of rRNA promoter complexes and potentiates their repression by DksA
Q36662639Regulation of bacterial RNA polymerase sigma factor activity: a structural perspective.
Q36208841Regulation of mammalian transcription by Gdown1 through a novel steric crosstalk revealed by cryo-EM
Q34659757Regulation of promoter-proximal transcription elongation: enhanced DNA scrunching drives λQ antiterminator-dependent escape from a σ70-dependent pause.
Q34055944Response of RNA polymerase to ppGpp: requirement for the omega subunit and relief of this requirement by DksA.
Q36081351Rifampin Resistance rpoB Alleles or Multicopy Thioredoxin/Thioredoxin Reductase Suppresses the Lethality of Disruption of the Global Stress Regulator spx in Staphylococcus aureus
Q35010831Role of the RNA polymerase sigma subunit in transcription initiation
Q46899395Role of the spacer between the -35 and -10 regions in sigmas promoter selectivity in Escherichia coli
Q41194509RpoN Modulates Carbapenem Tolerance in Pseudomonas aeruginosa through Pseudomonas Quinolone Signal and PqsE.
Q43244277Rsd family proteins make simultaneous interactions with regions 2 and 4 of the primary sigma factor
Q42574260Salerno's model of DNA re-analysed: could breather solitons have biological significance?
Q27655553Schizosacharomyces pombe RNA polymerase II at 3.6-A resolution
Q35889596Seeing is believing: the impact of structural genomics on antimicrobial drug discovery
Q39832015Sequential recognition of two distinct sites in sigma(S) by the proteolytic targeting factor RssB and ClpX.
Q28765495Sigma factors for cyanobacterial transcription
Q54512648Single DNA molecule analysis of transcription complexes.
Q42693159Single-strand promoter traps for bacterial RNA polymerase.
Q42137902Solute probes of conformational changes in open complex (RPo) formation by Escherichia coli RNA polymerase at the lambdaPR promoter: evidence for unmasking of the active site in the isomerization step and for large-scale coupled folding in the subse
Q38301292Specific recognition of the -10 promoter element by the free RNA polymerase sigma subunit
Q35012148Stationary phase gene regulation: what makes an Escherichia coli promoter sigmaS-selective?
Q36565042Still looking for the magic spot: the crystallographically defined binding site for ppGpp on RNA polymerase is unlikely to be responsible for rRNA transcription regulation
Q27653075Structural Basis for DNA-Hairpin Promoter Recognition by the Bacteriophage N4 Virion RNA Polymerase
Q27660054Structural and Biochemical Bases for the Redox Sensitivity of Mycobacterium tuberculosis RslA
Q27670850Structural and Mechanistic Basis for the Inhibition of Escherichia coli RNA Polymerase by T7 Gp2
Q27681958Structural basis for promoter specificity switching of RNA polymerase by a phage factor
Q27678856Structural basis for sigma factor mimicry in the general stress response of Alphaproteobacteria
Q27664035Structural basis for the bacterial transcription-repair coupling factor/RNA polymerase interaction
Q27689853Structural basis for the redox sensitivity of the Mycobacterium tuberculosis SigK-RskA σ-anti-σ complex
Q91809414Structural basis for transcription antitermination at bacterial intrinsic terminator
Q64108730Structural basis for transcription initiation by bacterial ECF σ factors
Q35129152Structural basis for transcription reactivation by RapA
Q61796620Structural basis of ECF-σ-factor-dependent transcription initiation
Q27678860Structural basis of a protein partner switch that regulates the general stress response of α-proteobacteria
Q24608460Structural basis of transcription initiation
Q27684481Structural basis of transcription initiation by bacterial RNA polymerase holoenzyme
Q27643138Structural basis of transcription: an RNA polymerase II-TFIIB cocrystal at 4.5 Angstroms
Q27676112Structural basis of transcriptional pausing in bacteria
Q34669788Structural biology of bacterial RNA polymerase
Q54708656Structural biology: New beginnings for transcription.
Q28757373Structural confirmation of a bent and open model for the initiation complex of T7 RNA polymerase
Q41627479Structural insights into the activity of enhancer-binding proteins.
Q51149172Structural insights into the unique mechanism of transcription activation by Caulobacter crescentus GcrA.
Q36416676Structural perspective on mutations affecting the function of multisubunit RNA polymerases
Q30332062Structural proteomics: the potential of high-throughput structure determination.
Q92503263Structural snapshots of actively transcribing influenza polymerase
Q35740383Structural, functional, and genetic analyses of the actinobacterial transcription factor RbpA
Q51107154Structure and Function of RNA Polymerases and the Transcription Machineries.
Q46802691Structure and carboxyl-terminal domain (CTD) binding of the Set2 SRI domain that couples histone H3 Lys36 methylation to transcription
Q27683438Structure and function of the initially transcribing RNA polymerase II-TFIIB complex
Q41629112Structure and function of the transcription elongation factor GreB bound to bacterial RNA polymerase
Q35758805Structure and mechanism of the RNA polymerase II transcription machinery
Q42637839Structure of RNA polymerase bound to ribosomal 30S subunit
Q27639929Structure of a T7 RNA polymerase elongation complex at 2.9 A resolution
Q36120805Structure of a bacterial RNA polymerase holoenzyme open promoter complex
Q27658448Structure of an RNA Polymerase II-TFIIB Complex and the Transcription Initiation Mechanism
Q39750259Structure-Function Studies of Escherichia coli RpoH (σ 32 ) by In Vitro Linker Insertion Mutagenesis
Q92309893Structures and mechanism of transcription initiation by bacterial ECF factors
Q24594359Structures of RNA polymerase-antibiotic complexes
Q38174426Studying DNA-protein interactions with single-molecule Förster resonance energy transfer
Q44462825Substitutions in the Escherichia coli RNA polymerase sigma70 factor that affect recognition of extended -10 elements at promoters
Q33758495Substrate requirements for regulated intramembrane proteolysis of Bacillus subtilis pro-sigmaK
Q27659599T7 phage protein Gp2 inhibits the Escherichia coli RNA polymerase by antagonizing stable DNA strand separation near the transcription start site
Q35758150TFB1 or TFB2 is sufficient for Thermococcus kodakaraensis viability and for basal transcription in vitro
Q28544090TFIIB is only ∼9 Å away from the 5'-end of a trimeric RNA primer in a functional RNA polymerase II preinitiation complex
Q34479824Temporal regulation of gene expression of the Thermus thermophilus bacteriophage P23-45.
Q42134644The -11A of promoter DNA and two conserved amino acids in the melting region of sigma70 both directly affect the rate limiting step in formation of the stable RNA polymerase-promoter complex, but they do not necessarily interact
Q45993100The C-terminal RpoN domain of sigma54 forms an unpredicted helix-turn-helix motif similar to domains of sigma70.
Q92933287The C-terminal tail of the yeast mitochondrial transcription factor Mtf1 coordinates template strand alignment, DNA scrunching and timely transition into elongation
Q39734953The FecI extracytoplasmic-function sigma factor of Escherichia coli interacts with the beta' subunit of RNA polymerase
Q27677778The Mechanism of E. coli RNA Polymerase Regulation by ppGpp Is Suggested by the Structure of their Complex
Q24643039The RNA polymerase "switch region" is a target for inhibitors
Q24656659The RNA polymerase II trigger loop functions in substrate selection and is directly targeted by alpha-amanitin
Q25257778The Rhizobium etli sigma70 (SigA) factor recognizes a lax consensus promoter
Q34400749The TFIIB Tip Domain Couples Transcription Initiation to Events Involved in RNA Processing
Q27649741The X-ray crystal structure of RNA polymerase from Archaea
Q40578873The Xp10 Bacteriophage Protein P7 Inhibits Transcription by the Major and Major Variant Forms of the Host RNA Polymerase via a Common Mechanism.
Q27677480The actinobacterial transcription factor RbpA binds to the principal sigma subunit of RNA polymerase
Q28077256The bacterial enhancer-dependent RNA polymerase
Q34353980The bacteriophage T4 inhibitor and coactivator AsiA inhibits Escherichia coli RNA Polymerase more rapidly in the absence of sigma70 region 1.1: evidence that region 1.1 stabilizes the interaction between sigma70 and core
Q36926930The bacteriophage lambda Q antiterminator protein contacts the beta-flap domain of RNA polymerase.
Q38011269The conflict between DNA replication and transcription
Q34559717The core-independent promoter-specific interaction of primary sigma factor
Q89113526The crystal structure of the RsbN-σBldN complex from Streptomyces venezuelae defines a new structural class of anti-σ factor
Q43020356The cyanobacterial principal sigma factor region 1.1 is involved in DNA-binding in the free form and in transcription activity as holoenzyme.
Q33722783The effects of upstream DNA on open complex formation by Escherichia coli RNA polymerase
Q39100789The essential activities of the bacterial sigma factor
Q33934477The interaction between sigma70 and the beta-flap of Escherichia coli RNA polymerase inhibits extension of nascent RNA during early elongation
Q34611578The interaction of Bacillus subtilis sigmaA with RNA polymerase
Q24681447The molecular basis of eukaryotic transcription
Q48328874The molecular basis of eukaryotic transcription (Nobel Lecture).
Q37070631The promoter spacer influences transcription initiation via sigma70 region 1.1 of Escherichia coli RNA polymerase
Q47960626The reduction in σ-promoter recognition flexibility as induced by core RNAP is required for σ to discern the optimal promoter spacing
Q35701605The regulation of bacterial transcription initiation
Q37357569The role of RNA polymerase sigma subunit in promoter-independent initiation of transcription.
Q38039049The role of bacterial enhancer binding proteins as specialized activators of σ54-dependent transcription
Q44209223The role of the alarmone (p)ppGpp in sigma N competition for core RNA polymerase.
Q38400726The sabotage of the bacterial transcription machinery by a small bacteriophage protein.
Q36226866The second paradigm for activation of transcription.
Q47661886The sigma 70 subunit of RNA polymerase induces lacUV5 promoter-proximal pausing of transcription
Q47661898The sigma 70 subunit of RNA polymerase mediates a promoter-proximal pause at the lac promoter.
Q43445695The sigma(70) subunit of RNA polymerase is contacted by the (lambda)Q antiterminator during early elongation
Q21999783The sigma70 family of sigma factors
Q27652631The structure of a transcribing T7 RNA polymerase in transition from initiation to elongation
Q27671645The structure of a transcription activation subcomplex reveals how σ70is recruited to PhoB promoters
Q35018019The third dimension for protein interactions and complexes.
Q42118811The transcription inhibitor lipiarmycin blocks DNA fitting into the RNA polymerase catalytic site
Q39986911The σ70 region 1.2 regulates promoter escape by unwinding DNA downstream of the transcription start site
Q42599486Thermoirreversible and Thermoreversible Promoter Opening by Two Escherichia coli RNA Polymerase Holoenzymes
Q46946945Topography of the euryarchaeal transcription initiation complex
Q53115968Towards quantitative analysis of gene regulation by enhancers.
Q37034702Transcription initiation by mix and match elements: flexibility for polymerase binding to bacterial promoters
Q34332129Transcription of the T4 late genes
Q34332135Transcriptional control in the prereplicative phase of T4 development
Q39680309Uncovering ancient transcription systems with a novel evolutionary indicator.
Q34870092Unusual interaction of RNA polymerase with the bacteriophage Mu middle promoter Pm in the absence of its activator protein Mor.
Q54512624Using a lac repressor roadblock to analyze the E. coli transcription elongation complex.
Q40916082Utilization of variably spaced promoter-like elements by the bacterial RNA polymerase holoenzyme during early elongation
Q38357484Visualizing RNA extrusion and DNA wrapping in transcription elongation complexes of bacterial and eukaryotic RNA polymerases
Q27322936Visualizing the phage T4 activated transcription complex of DNA and E. coli RNA polymerase
Q27676227X-ray Crystal Structure of Escherichia coli RNA Polymerase  70 Holoenzyme
Q39121534σ38-dependent promoter-proximal pausing by bacterial RNA polymerase
Q38024620σ70 and PhoB activator: getting a better grip.

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