Mutational analysis of pre-mRNA splicing in Saccharomyces cerevisiae using a sensitive new reporter gene, CUP1

scientific article

Mutational analysis of pre-mRNA splicing in Saccharomyces cerevisiae using a sensitive new reporter gene, CUP1 is …
instance of (P31):
scholarly articleQ13442814

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P932PMC publication ID1205405
P698PubMed publication ID8462846

P2093author name stringC Guthrie
C F Lesser
P2860cites workDNA sequencing with chain-terminating inhibitorsQ22066207
Early commitment of yeast pre-mRNA to the spliceosome pathwayQ24627964
High efficiency transformation of E. coli by high voltage electroporationQ29615278
Messenger RNA Splicing in Yeast: Clues to Why the Spliceosome Is a RibonucleoproteinQ29618253
A compensatory base change in U1 snRNA suppresses a 5' splice site mutationQ29618292
Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicingQ29620837
Mechanism for cryptic splice site activation during pre-mRNA splicingQ33746053
A putative ATP binding protein influences the fidelity of branchpoint recognition in yeast splicingQ33838525
Interference of nonsense mutations with eukaryotic messenger RNA stabilityQ34037213
Primary structure and transcription of an amplified genetic locus: the CUP1 locus of yeastQ36246632
Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiaeQ36387630
Isolation and sequence of the gene for actin in Saccharomyces cerevisiaeQ36396688
Industrial yeasts display tandem gene iteration at the CUP1 regionQ36977303
Biochemical Mechanisms of Constitutive and Regulated Pre-mRNA SplicingQ37132263
Nuclear pre-mRNA splicing in yeastQ38762332
Homologous copper(1)-(thiolate)2-chromophores in yeast copper thioneinQ40796107
A U1 snRNA:pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5' cleavage site.Q41107145
5' splice site selection in yeast: genetic alterations in base-pairing with U1 reveal additional requirementsQ44055786
A trans-acting suppressor restores splicing of a yeast intron with a branch point mutationQ44378546
An early hierarchic role of U1 small nuclear ribonucleoprotein in spliceosome assembly.Q45985841
A U-rich tract enhances usage of an alternative 3′ splice site in yeastQ46196387
Cloning of random-sequence oligodeoxynucleotidesQ46346837
Preparation and analysis of low molecular weight RNAs and small ribonucleoproteinsQ46552136
Meiosis-specific RNA splicing in yeastQ48209478
Recovery of plasmids from yeast into Escherichia coli: shuttle vectors.Q54702408
A quantitative analysis of the effects of 5′ junction and TACTAAC box mutants and mutant combinations on yeast mRNA splicingQ57909448
Hierarchy for 5′ splice site preference determined in vivoQ58830815
U1 snRNP can influence 3'-splice site selection as well as 5'-splice site selectionQ67901761
Mutations in a yeast intron demonstrate the importance of specific conserved nucleotides for the two stages of nuclear mRNA splicingQ68864416
A role for exon sequences and splice-site proximity in splice-site selectionQ68970669
5' cleavage site in eukaryotic pre-mRNA splicing is determined by the overall 5' splice region, not by the conserved 5' GUQ69336145
Mutational analysis of the interactions between U1 small nuclear RNA and pre-mRNA of yeastQ69554872
Splice site selection and ribonucleoprotein complex assembly during in vitro pre-mRNA splicingQ69829038
Function and autoregulation of yeast copperthioneinQ69890822
Molecular consequences of specific intron mutations on yeast mRNA splicing in vivo and in vitroQ69899506
A point mutation in the conserved hexanucleotide at a yeast 5' splice junction uncouples recognition, cleavage, and ligationQ70085295
High-efficiency transformation of yeast by electroporationQ70120808
Vectors for constitutive and inducible gene expression in yeastQ70120830
Mutations in yeast U5 snRNA alter the specificity of 5' splice-site cleavageQ70135657
Saccharomyces cerevisiae actin--Escherichia coli lacZ gene fusions: synthetic-oligonucleotide-mediated deletion of the 309 base pair intervening sequence in the actin geneQ70437309
P433issue4
P407language of work or nameEnglishQ1860
P921main subjectSaccharomyces cerevisiaeQ719725
mutational analysisQ1955810
P304page(s)851-863
P577publication date1993-04-01
P1433published inGeneticsQ3100575
P1476titleMutational analysis of pre-mRNA splicing in Saccharomyces cerevisiae using a sensitive new reporter gene, CUP1
P478volume133

Reverse relations

cites work (P2860)
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Q37419197Exon sequences at the splice junctions affect splicing fidelity and alternative splicing
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Q88645751Functional analysis of Hsh155/SF3b1 interactions with the U2 snRNA/branch site duplex
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Q24537373Prp8 protein: at the heart of the spliceosome
Q27937907Prp8 retinitis pigmentosa mutants cause defects in the transition between the catalytic steps of splicing
Q36712610Pseudouridines in U2 snRNA stimulate the ATPase activity of Prp5 during spliceosome assembly
Q34157560Pseudouridylation goes regulatory
Q27000261RNA helicases in splicing
Q36717834RNA pseudouridylation: new insights into an old modification
Q37705801Rapid identification of mRNA processing defects with a novel single-cell yeast reporter
Q27939473Rearrangement of competing U2 RNA helices within the spliceosome promotes multiple steps in splicing
Q42118082Remodeling of U2-U6 snRNA helix I during pre-mRNA splicing by Prp16 and the NineTeen Complex protein Cwc2
Q36564321Repression of gene expression by an exogenous sequence element acting in concert with a heterogeneous nuclear ribonucleoprotein-like protein, Nrd1, and the putative helicase Sen1
Q39085957Role of Cwc24 in the First Catalytic Step of Splicing and Fidelity of 5' Splice Site Selection
Q27668022Role of the ubiquitin-like protein Hub1 in splice-site usage and alternative splicing
Q37587994SF3B1/Hsh155 HEAT motif mutations affect interaction with the spliceosomal ATPase Prp5, resulting in altered branch site selectivity in pre-mRNA splicing
Q33635951SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast
Q35447518SUS1 introns are required for efficient mRNA nuclear export in yeast
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Q39826068Secondary structure is required for 3' splice site recognition in yeast
Q36321221Septin-dependent compartmentalization of the endoplasmic reticulum during yeast polarized growth
Q35863719Sequencing of lariat termini in S. cerevisiae reveals 5' splice sites, branch points, and novel splicing events
Q36540298Snu56p is required for Mer1p-activated meiotic splicing
Q88571202Spliceosome Profiling Visualizes Operations of a Dynamic RNP at Nucleotide Resolution
Q33933327Spliceosome discards intermediates via the DEAH box ATPase Prp43p
Q34362457Splicing enhancement in the yeast rp51b intron
Q27936858Splicing proofreading at 5' splice sites by ATPase Prp28p
Q47593055Stress-induced Pseudouridylation Alters the Structural Equilibrium of Yeast U2 snRNA Stem II.
Q38833557Structural toggle in the RNaseH domain of Prp8 helps balance splicing fidelity and catalytic efficiency
Q27934955The Isy1p component of the NineTeen complex interacts with the ATPase Prp16p to regulate the fidelity of pre-mRNA splicing
Q35783047The Prp18 protein stabilizes the interaction of both exons with the U5 snRNA during the second step of pre-mRNA splicing
Q33771893The Saccharomyces cerevisiae RAD9 checkpoint reduces the DNA damage-associated stimulation of directed translocations
Q27934683The Saccharomyces cerevisiae gene CDC40/PRP17 controls cell cycle progression through splicing of the ANC1 gene
Q38289890The amino-terminal domain of yeast U1-70K is necessary and sufficient for function
Q39934103The biflavonoid isoginkgetin is a general inhibitor of Pre-mRNA splicing
Q40523065The interaction between the first and last intron nucleotides in the second step of pre-mRNA splicing is independent of other conserved intron nucleotides
Q36429942The spliceosome: a flexible, reversible macromolecular machine
Q39687383Thermoconditional modulation of the pleiotropic sensitivity phenotype by the Saccharomyces cerevisiae PRP19 mutant allele pso4-1.
Q34485772U2 snRNA is inducibly pseudouridylated at novel sites by Pus7p and snR81 RNP.
Q35720890U2 toggles iteratively between the stem IIa and stem IIc conformations to promote pre-mRNA splicing
Q27930796cis- and trans-Acting determinants of transcription termination by yeast RNA polymerase II
Q27935241prp8 mutations that cause human retinitis pigmentosa lead to a U5 snRNP maturation defect in yeast
Q42381501trans-splicing to spliceosomal U2 snRNA suggests disruption of branch site-U2 pairing during pre-mRNA splicing

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