Purification of Tetrahymena telomerase and cloning of genes encoding the two protein components of the enzyme

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Purification of Tetrahymena telomerase and cloning of genes encoding the two protein components of the enzyme is …
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scholarly articleQ13442814

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P356DOI10.1016/0092-8674(95)90529-4
P698PubMed publication ID7774009
P5875ResearchGate publication ID15425204

P50authorCarol GreiderQ26322
P2093author name stringKobayashi R
Collins K
P2860cites workThe DNA of ciliated protozoaQ24634620
Basic local alignment search toolQ25938991
Specific Association of Human Telomerase Activity with Immortal Cells and CancerQ27860801
A mutant with a defect in telomere elongation leads to senescence in yeastQ27930429
Structure and functional properties of human general transcription factor IIEQ28115411
Telomerase RNAs of different ciliates have a common secondary structure and a permuted templateQ28240524
Transcription factors IIE and IIH and ATP hydrolysis direct promoter clearance by RNA polymerase IIQ28646845
A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesisQ29614741
Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activityQ29615388
Telomere length predicts replicative capacity of human fibroblastsQ29615745
Identification of four conserved motifs among the RNA-dependent polymerase encoding elementsQ29617964
TLC1: template RNA component of Saccharomyces cerevisiae telomeraseQ29618197
Photocrosslinking of 5-Iodouracil-Substituted RNA and DNA to ProteinsQ30464738
Functional Evidence for an RNA Template in TelomeraseQ33998306
Structural motifs and potential sigma homologies in the large subunit of human general transcription factor TFIIE.Q34989722
Telomerase activity in human ovarian carcinomaQ35142569
Oligonucleotides complementary to the Oxytricha nova telomerase RNA delineate the template domain and uncover a novel mode of primer utilizationQ36671065
Chromosome first aid.Q37430022
Architecture of telomerase RNA.Q37639292
Gel shift and UV cross-linking analysis of Tetrahymena telomeraseQ38296586
Sequence-specific DNA primer effects on telomerase polymerization activityQ40656443
Telomeres: no end in sightQ40763655
An attempt to unify the structure of polymerasesQ41204694
A conserved secondary structure for telomerase RNAQ44060757
RNA Polymerase Marching BackwardQ46558907
In vivo alteration of telomere sequences and senescence caused by mutated Tetrahymena telomerase RNAsQ46821624
The internally located telomeric sequences in the germ-line chromosomes of Tetrahymena are at the ends of transposon-like elementsQ48372823
Codon usage in Tetrahymena and other ciliatesQ52867193
Functional reconstitution of wild-type and mutant Tetrahymena telomeraseQ71650294
Tetrahymena telomerase catalyzes nucleolytic cleavage and nonprocessive elongationQ72829853
P433issue5
P407language of work or nameEnglishQ1860
P304page(s)677-686
P577publication date1995-06-01
P1433published inCellQ655814
P1476titlePurification of Tetrahymena telomerase and cloning of genes encoding the two protein components of the enzyme
P478volume81

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cites work (P2860)
Q37062421A Conserved Telomerase Motif within the Catalytic Domain of Telomerase Reverse Transcriptase Is Specifically Required for Repeat Addition Processivity
Q36071305A functional telomerase RNA swap in vivo reveals the importance of nontemplate RNA domains
Q28595030A mammalian telomerase-associated protein
Q41138846A rapid, useful and quantitative method to measure telomerase activity by hybridization protection assay connected with a telomeric repeat amplification protocol
Q51762251A review of telomere length in sarcopenia and frailty.
Q40018656A single telomerase RNA is sufficient for the synthesis of variable telomeric DNA repeats in ciliates of the genus Paramecium
Q43636619A stem-loop of Tetrahymena telomerase RNA distant from the template potentiates RNA folding and telomerase activity
Q72036720ATP-dependent Processivity of a Telomerase Activity from Saccharomyces cerevisiae
Q57250868Accelerated loss of telomeric repeats may not explain accelerated replicative decline of Werner syndrome cells
Q34428321Affinity purification of DNA-binding proteins
Q67213534Amplification, increased dosage and in situ expression of the telomerase RNA gene in human cancer
Q38294059An in vitro assay for saccharomyces telomerase requires EST1
Q34420492Analysis of telomerase in Candida albicans: potential role in telomere end protection
Q42679147Aquarius, a novel gene isolated by gene trapping with an RNA-dependent RNA polymerase motif
Q37677320Association of the Est1 protein with telomerase activity in yeast
Q35131769Biochemical aspects of telomerase function
Q40157259Biochemical properties of Trypanosoma cruzi telomerase
Q46980175Biological and clinical implications of telomere dysfunction in myeloid malignancies
Q71738168Boundary elements of the Tetrahymena telomerase RNA template and alignment domains
Q24814974CTCF binds the proximal exonic region of hTERT and inhibits its transcription
Q33925593Cell proliferation, carcinogenesis and diverse mechanisms of telomerase regulation
Q53221552Characterization of Trypanosoma cruzi telomerase
Q36581467Characterization of human telomerase complex.
Q33965566Characterization of the interaction between the nuclease and reverse transcriptase activity of the yeast telomerase complex
Q41125620Chromosomal instability and alteration of telomere repeat sequences.
Q41270223Chromosome healing: spontaneous and programmed de novo telomere formation by telomerase
Q33953553Ciliate telomerase biochemistry
Q34065693Comparative Gene Mapping of the Human and Mouse TEP1 Genes, Which Encode One Protein Component of Telomerases
Q38360360Conserved N-terminal motifs of telomerase reverse transcriptase required for ribonucleoprotein assembly in vivo
Q33973729Correlation between expression of human telomerase subunits and telomerase activity in esophageal squamous cell carcinoma
Q41720377DNA dynamics: Different means to a common end?
Q33886150De novo telomere addition by Tetrahymena telomerase in vitro
Q48599863Developmental regulation of telomerase activity in human fetal tissues during gestation
Q35209661Developmentally programmed assembly of higher order telomerase complexes with distinct biochemical and structural properties
Q33886575Developmentally regulated initiation of DNA synthesis by telomerase: evidence for factor-assisted de novo telomere formation
Q56508142Differential regulation of telomerase activity and telomerase RNA during multi-stage tumorigenesis
Q24301904Differential regulation of telomerase activity by six telomerase subunits
Q33890968Disease states associated with telomerase deficiency appear earlier in mice with short telomeres
Q42715561Do telomerase antagonists represent a novel anti-cancer strategy?
Q74457481Effect of DNA Secondary Structure on Human Telomerase Activity
Q54322792Eimeria tenella: 14-3-3 protein interacts with telomerase
Q73149412Enzymatic activity of endogenous telomerase associated with intact nuclei from human leukemia CEM cells
Q27931251Est1 has the properties of a single-stranded telomere end-binding protein
Q30471962Euplotes Telomerase: Evidence for Limited Base-Pairing during Primer Elongation and dGTP as an Effector of Translocation
Q30306373Euplotes telomerase contains an La motif protein produced by apparent translational frameshifting
Q35021955Ever shorter telomere 1 ( EST1 )-dependent reverse transcription by Candida telomerase in vitro : Evidence in support of an activating function
Q58866811Evolutionary rates of mammalian telomere-stability genes correlate with karyotype features and female germline expression
Q55346573Expression of human telomerase RNA is an early event of stomach carcinogenesis.
Q74601071Expression of human telomerase subunits in ovarian malignant, borderline and benign tumors
Q37470406Expression of mouse telomerase catalytic subunit in embryos and adult tissues.
Q33957757Expression of mutated Paramecium telomerase RNAs in vivo leads to templating errors that resemble those made by retroviral reverse transcriptase
Q30779078Expression of telomerase subunits in normal and neoplastic prostate epithelial cells isolated by laser capture microdissection
Q33772647Flexible Positioning of the Telomerase-Associated Nuclease Leads to Preferential Elimination of Nontelomeric DNA
Q91742198Functional Proteomics of Nuclear Proteins in Tetrahymena thermophila: A Review
Q38296299Functional analysis of conserved residues in the putative "finger" domain of telomerase reverse transcriptase
Q74549782Functional analysis of the C-terminal extension of telomerase reverse transcriptase. A putative "thumb" domain
Q33969441Functional multimerization of the human telomerase reverse transcriptase
Q35194966Functionally interacting telomerase RNAs in the yeast telomerase complex
Q77935987Genes involved in the control of cellular proliferative potential
Q40953506Genomic organization and promoter characterization of the gene encoding the human telomerase reverse transcriptase (hTERT).
Q40882874Holoenzyme proteins required for the physiological assembly and activity of telomerase
Q24533247Human telomerase and its regulation
Q24604009Human telomerase contains evolutionarily conserved catalytic and structural subunits
Q77959517Human telomerase reverse transcriptase as a critical determinant of telomerase activity in normal and malignant endometrial tissues
Q36786230ISWI, a member of the SWI2/SNF2 ATPase family, encodes the 140 kDa subunit of the nucleosome remodeling factor
Q39721592Identification and characterization of a telomerase activity from Schizosaccharomyces pombe
Q38342698Identification of Determinants for Inhibitor Binding within the RNA Active Site of Human Telomerase Using PNA Scanning
Q63384208Identification of Functional Domains and Dominant Negative Mutations in Vertebrate Telomerase RNA Using anin VivoReconstitution System
Q39575704Identification of Kluyveromyces lactis Telomerase: Discontinuous Synthesis along the 30-Nucleotide-Long Templating Domain
Q33964637Identification of functionally important domains in the N-terminal region of telomerase reverse transcriptase
Q24548517Identification of two RNA-binding proteins associated with human telomerase RNA
Q78654076Implication of the exon region in the regulation of the human telomerase reverse transcriptase gene promoter
Q40422066In vivo synthesis of nano-selenium by Tetrahymena thermophila SB210.
Q28366118Increased epidermal tumors and increased skin wound healing in transgenic mice overexpressing the catalytic subunit of telomerase, mTERT, in basal keratinocytes.
Q47960865Inhibition of human telomerase activity by peptide nucleic acids
Q60589823Inhibition of telomerase activity by a cell-penetrating peptide nucleic acid construct in human melanoma cells
Q35193095Interaction of recombinant Tetrahymena telomerase proteins p80 and p95 with telomerase RNA and telomeric DNA substrates
Q38502576Interaction of the Nuclear Matrix-associated Region (MAR)-Binding Proteins, SATB1 and CDP/Cux, with a MAR Element (L2a) in an Upstream Regulatory Region of the Mouse CD8a Gene
Q38311992Interference footprinting analysis of telomerase elongation complexes
Q41610038Mammalian Telomerase: Catalytic Subunit and Knockout Mice
Q48028040Mapping of the Gene for Rat Telomerase Protein Component 1(Tlp1) to Chromosome 15
Q41441652Mechanisms of cellular senescence
Q34081958Microenvironmental regulation of telomerase isoforms in human embryonic stem cells
Q24545497Mini- and microsatellites
Q24537660Minimum length requirement of the alignment domain of human telomerase RNA to sustain catalytic activity in vitro
Q42609119Molecular cloning and characterization of AtTERT, a telomerase reverse transcriptase homolog in Arabidopsis thaliana
Q28118564Molecular interactions between telomerase and the tumor suppressor protein p53 in vitro
Q41730873Mouse models for the study of telomerase.
Q34655881Mutational analysis of the Tetrahymena telomerase RNA: identification of residues affecting telomerase activity in vitro
Q38338378Negative regulation of yeast telomerase activity through an interaction with an upstream region of the DNA primer
Q39724301One-step affinity purification protocol for human telomerase
Q42576145Organization of chromosome ends in Ustilago maydis. RecQ-like helicase motifs at telomeric regions.
Q36835046Permanent embryo arrest: molecular and cellular concepts
Q34595148Physical Activity and Telomere Biology: Exploring the Link with Aging-Related Disease Prevention
Q24550780Polymerization defects within human telomerase are distinct from telomerase RNA and TEP1 binding
Q73876649Possible mechanisms for the regulation of telomere length
Q40791502Preparation of monoclonal antibodies against human telomerase
Q36560631Processing of nontelomeric 3' ends by telomerase: default template alignment and endonucleolytic cleavage
Q34790751Prospects for anti-neoplastic therapies based on telomere biology
Q73456230Protein phosphatase 2A inhibits nuclear telomerase activity in human breast cancer cells
Q30469368Purification of telomerase from Euplotes aediculatus: requirement of a primer 3' overhang
Q47848808Putative telomerase catalytic subunits from Giardia lamblia and Caenorhabditis elegans
Q35912728Reconstitution of human telomerase activity and identification of a minimal functional region of the human telomerase RNA.
Q28116966Reconstitution of human telomerase activity in vitro
Q24313396Reconstitution of human telomerase with the template RNA component hTR and the catalytic protein subunit hTRT
Q77293489Regulation of catalytic activity and processivity of human telomerase
Q34675873Reiterative dG addition by Euplotes crassus telomerase during extension of non-telomeric DNA
Q34451265Relics from the RNA World
Q29618392Reverse transcriptase motifs in the catalytic subunit of telomerase
Q37380017Reversible immortalization of mammalian cells mediated by retroviral transfer and site-specific recombination
Q30471858Reversing Time: Origin of Telomerase
Q41701216Review Chromosome healing, telomere capture and mechanisms of radiation-induced chromosome breakage
Q34130110Ribonucleoprotein multimers and their functions
Q35956258Role of the casein kinase I isoform, Hrr25, and the cell cycle-regulatory transcription factor, SBF, in the transcriptional response to DNA damage in Saccharomyces cerevisiae
Q27931258Senescence mutants of Saccharomyces cerevisiae with a defect in telomere replication identify three additional EST genes.
Q38324157Sequence-specific and conformation-dependent binding of yeast telomerase RNA to single-stranded telomeric DNA
Q34659594Species-specific and sequence-specific recognition of the dG-rich strand of telomeres by yeast telomerase
Q36556655Specific RNA residue interactions required for enzymatic functions of Tetrahymena telomerase
Q35210631Specific telomerase RNA residues distant from the template are essential for telomerase function
Q41066960Structure and function of telomerase
Q40955340Structure, function, and replication of Saccharomyces cerevisiae telomeres
Q37472153Structure-function relationships in telomerase genes.
Q33792301Studies of the molecular mechanisms in the regulation of telomerase activity
Q28564535TLP1: a gene encoding a protein component of mammalian telomerase is a novel member of WD repeats family
Q41071112Telomerase Activity in Human Leukemic Cell Lines Is Inhibited by Antisense Pentadecadeoxynucleotides Targeted against c-myc mRNA
Q29615387Telomerase Catalytic Subunit Homologs from Fission Yeast and Human
Q35203518Telomerase RNA function in recombinant Tetrahymena telomerase
Q40915193Telomerase activation and cancer.
Q35763644Telomerase activation in cervical cancer.
Q48905543Telomerase activity and telomere detection during early bovine development
Q40977667Telomerase activity does not always imply telomere maintenance
Q73016390Telomerase activity in bladder carcinoma and its implication for noninvasive diagnosis by detection of exfoliated cancer cells in urine
Q77578762Telomerase activity in gestational trophoblastic disease and placental tissue from early and late human pregnancies
Q77317980Telomerase activity in ‘immortal’ fish1
Q77729120Telomerase activity, MIB-1, PCNA, HPV 16 and p53 as diagnostic markers for cervical intraepithelial neoplasia
Q57129041Telomerase and cancer
Q30472155Telomerase and chromosome end maintenance.
Q41560700Telomerase and telomere-binding proteins: controlling the endgame.
Q30471909Telomerase and the Chromosome end Replication Problem
Q36068547Telomerase catalysis: A phylogenetically conserved reverse transcriptase
Q33533965Telomerase enzyme activation and human cell immortalization
Q77408401Telomerase from Saccharomyces cerevisiae contains several protein subunits and may have different activities depending on the protein content
Q46450385Telomerase in cancer: clinical applications
Q32182322Telomerase in gynecologic cancers
Q34068018Telomerase is controlled by protein kinase Calpha in human breast cancer cells
Q38328772Telomerase protein rather than its RNA is the target of phosphorothioate-modified oligonucleotides
Q30472352Telomerase reverse transcriptase genes identified in Tetrahymena thermophila and Oxytricha trifallax
Q77892451Telomerase, Ku, and telomeric silencing in Saccharomyces cerevisiae
Q34148457Telomerase, immortality and cancer
Q28140790Telomerase-associated protein TEP1 is not essential for telomerase activity or telomere length maintenance in vivo
Q80600068Telomerase: a dimeric ménage à trois
Q41239266Telomerase: anti-cancer target or just a fascinating enzyme?
Q33885734Telomerase: therapeutic potential in cancer
Q77331385Telomere Structure, Replication and Length Maintenance
Q40893289Telomere crisis in leukemia
Q38127519Telomere length and telomerase activity during oocyte maturation and early embryo development in mammalian species
Q48589695Telomere length and telomerase activity in bovine pre-implantation embryos in vitro
Q36255827Telomere length dynamics and chromosomal instability in cells derived from telomerase null mice
Q28290143Telomere length regulation
Q33540084Telomere maintenance mechanisms and cellular immortalization.
Q34293590Telomere maintenance mechanisms as a target for drug development
Q24598974Telomere shortening and apoptosis in telomerase-inhibited human tumor cells
Q33890481Telomere shortening in mTR-/- embryos is associated with failure to close the neural tube
Q59287960Telomeres
Q33766334Telomeres and cell division potential
Q71870874Telomeres and disease
Q41149926Telomeres and telomerase in human hematologic neoplasia
Q41363085Telomeres and telomerase in normal and malignant haematologic cells
Q71738792Telomeres and telomerase: a simple picture becomes complex
Q33669749Telomeres and telomerase: more than the end of the line
Q34750440Telomeres and telomerases as drug targets
Q34090772Telomeres and their control
Q38101746Telomeres in lung disease
Q54609545Telomeres, Telomerase and Leukaemia.
Q33825177Telomeres, telomerase, and myc. An update
Q41744090Telomeres, telomerase, and the cell cycle
Q42566913Tetrahymena Mutants With Short Telomeres
Q33741625Tetrahymena genetics: two nuclei are better than one.
Q27937778Tetrahymena histone acetyltransferase A: a homolog to yeast Gcn5p linking histone acetylation to gene activation
Q30453860Tetrahymena proteins p80 and p95 are not core telomerase components
Q39728063Tetrahymena telomerase ribonucleoprotein RNA-protein interactions
Q33786914The Est1 subunit of yeast telomerase binds the Tlc1 telomerase RNA
Q27938022The SAP, a new family of proteins, associate and function positively with the SIT4 phosphatase
Q24793277The TROVE module: a common element in Telomerase, Ro and Vault ribonucleoproteins
Q46015316The Tetrahymena p80/p95 complex is required for proper telomere length maintenance and micronuclear genome stability.
Q28776783The anchor site of telomerase from Euplotes aediculatus revealed by photo-cross-linking to single- and double-stranded DNA primers
Q36558592The catalytic subunit of yeast telomerase
Q41042767The chromosome ends of Saccharomyces cerevisiae.
Q80818316The identification of telomerase subunits: catalysing telomere research
Q41583649The implications of telomerase biochemistry for human disease
Q24294488The p80 homology region of TEP1 is sufficient for its association with the telomerase and vault RNAs, and the vault particle
Q34451259The path from the RNA world.
Q36199868The reverse transcriptase component of the Tetrahymena telomerase ribonucleoprotein complex
Q41216084The roles of telomeres and telomerase in cell life span
Q36377506The telomerase RNA pseudoknot is critical for the stable assembly of a catalytically active ribonucleoprotein
Q22065785The telomerase reverse transcriptase: components and regulation
Q61959832The use of synthetic genes for the expression of ciliate proteins in heterologous systems
Q30471100Three Ever Shorter Telomere (EST) genes are dispensable for in vitro yeast telomerase activity
Q33959420Two inactive fragments of the integral RNA cooperate to assemble active telomerase with the human protein catalytic subunit (hTERT) in vitro
Q40413921Unusual reverse transcriptases
Q73170467Up-regulation of telomerase in human B lymphocytes occurs independently of cellular proliferation and with expression of the telomerase catalytic subunit
Q37624549Utilization of ribonucleotides and RNA primers by Tetrahymena telomerase.
Q42077815Variable telomeric repeat synthesis in Paramecium tetraurelia is consistent with misincorporation by telomerase
Q24294689Vault poly(ADP-ribose) polymerase is associated with mammalian telomerase and is dispensable for telomerase function and vault structure in vivo
Q35015678Vault ribonucleoprotein particles: sarcophagi, gondolas, or safety deposit boxes?
Q37067670Yeast telomerase is capable of limited repeat addition processivity
Q30470926dGTP-dependent processivity and possible template switching of Euplotes telomerase
Q24310670hEST2, the Putative Human Telomerase Catalytic Subunit Gene, Is Up-Regulated in Tumor Cells and during Immortalization
Q71387702rTP: a candidate telomere protein that is associated with DNA replication

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