A set of lacZ mutations in Escherichia coli that allow rapid detection of each of the six base substitutions

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A set of lacZ mutations in Escherichia coli that allow rapid detection of each of the six base substitutions is …
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scholarly articleQ13442814

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P819ADS bibcode1989PNAS...86.5345C
P356DOI10.1073/PNAS.86.14.5345
P932PMC publication ID297618
P698PubMed publication ID2501784
P5875ResearchGate publication ID20653926

P2093author name stringJ H Miller
C G Cupples
P2860cites workCarcinogens are mutagens: a simple test system combining liver homogenates for activation and bacteria for detectionQ24564216
Detection of carcinogens as mutagens: bacterial tester strains with R factor plasmidsQ24564599
ON THE TOPOGRAPHY OF THE GENETIC FINE STRUCTUREQ24644905
Genetic regulatory mechanisms in the synthesis of proteinsQ28186173
The mutY gene: a mutator locus in Escherichia coli that generates G.C----T.A transversionsQ33567384
Effects of amino acid substitutions at the active site in Escherichia coli beta-galactosidaseQ33954766
Molecular basis of base substitution hotspots in Escherichia coliQ34183770
Spectra of spontaneous mutations in Escherichia coli strains defective in mismatch correction: the nature of in vivo DNA replication errors.Q34343418
Colony probing as an alternative to standard sequencing as a means of direct analysis of chromosomal DNA to determine the spectrum of single-base changes in regions of known sequenceQ35590677
mutM, a second mutator locus in Escherichia coli that generates G.C----T.A transversionsQ36223680
Creation of a test plasmid for detecting G-C-to-T-A transversions by changing serine to arginine in the active site of beta-lactamaseQ36237266
Analysis of spontaneous base substitutions generated in mismatch-repair-deficient strains of Escherichia coliQ36259051
The unusual mutagenic specificity of an E. Coli mutator geneQ36456280
Mutational specificity in bacteriaQ40169820
The biochemistry of mutagenesisQ40596841
Genetic studies of the lac repressor. VII. On the molecular nature of spontaneous hotspots in the lacI gene of Escherichia coliQ41008292
Spontaneous mutagenesis in Escherichia coli strains lacking 6-methyladenine residues in their DNA an altered mutational spectrum in dam− mutantsQ41021768
Genetic studies of the lac repressor. IV. Mutagenic specificity in the lacI gene of Escherichia coliQ41379292
Genetic studies of the lac repressor. III. Additional correlation of mutational sites with specific amino acid residuesQ42028460
Specificity of UV mutagenesis in the lac promoter of M13lac hybrid phage DNA.Q48404264
Classifying mutagens as to their specificity in causing the six possible transitions and transversions: a simple analysis using the Salmonella mutagenicity assayQ50204623
Mutagenic specificity: reversion of iso-1-cytochrome c mutants of yeastQ68669502
Specific induction of transitions and transversions of G-C base pairs by 4-nitroquinoline-1-oxide in iso-1-cytochrome c mutants of yeastQ68687863
Amino acid replacements and the genetic codeQ69834535
The specificity of base-pair substitution induced by the mutL and mutS mutators in E. coliQ69884370
Mutagenic specificity of ultraviolet lightQ69946985
Further correlations of the lacI genetic map with the DNA sequenceQ70226481
Identification of an essential carboxylate group at the active site of lacZ beta-galactosidase from Escherichia coliQ70462482
A determination of mutagen specificity in bacteria using nonsense mutants of bacteriophage T4Q72231657
Changes in DNA base sequence induced by targeted mutagenesis of lambda phage by ultraviolet lightQ72808155
P433issue14
P407language of work or nameEnglishQ1860
P921main subjectEscherichia coliQ25419
P304page(s)5345-5349
P577publication date1989-07-01
P1433published inProceedings of the National Academy of Sciences of the United States of AmericaQ1146531
P1476titleA set of lacZ mutations in Escherichia coli that allow rapid detection of each of the six base substitutions
P478volume86

Reverse relations

cites work (P2860)
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Q54068048Endonuclease V protects Escherichia coli against specific mutations caused by nitrous acid.
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Q28205159Enzymes associated with reductive activation and action of nitazoxanide, nitrofurans, and metronidazole in Helicobacter pylori
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Q35966713Escherichia coli DNA polymerase II catalyzes chromosomal and episomal DNA synthesis in vivo
Q39725401Escherichia coli MutY protein has a guanine-DNA glycosylase that acts on 7,8-dihydro-8-oxoguanine:guanine mispair to prevent spontaneous G:C-->C:G transversions
Q24594473Escherichia coli Nth and human hNTH1 DNA glycosylases are involved in removal of 8-oxoguanine from 8-oxoguanine/guanine mispairs in DNA
Q39793949Escherichia coli cells bearing a ribosomal ambiguity mutation in rpsD have a mutator phenotype that correlates with increased mistranslation
Q34615970Escherichia coli strains (ndk) lacking nucleoside diphosphate kinase are powerful mutators for base substitutions and frameshifts in mismatch-repair-deficient strains
Q44615570Evaluation of mutagenic activity in an extract of pepper tree stem bark (Schinus terebinthifolius Raddi).
Q42089149Evidence for mutagenesis by nitric oxide during nitrate metabolism in Escherichia coli
Q37132610Evidence that MutY and MutM combine to prevent mutations by an oxidatively damaged form of guanine in DNA
Q38193327Evolutionary engineering by genome shuffling
Q33739839Examination of the role of DNA polymerase proofreading in the mutator effect of miscoding tRNAs.
Q28610597Excision of hypoxanthine from DNA containing dIMP residues by the Escherichia coli, yeast, rat, and human alkylpurine DNA glycosylases
Q43967548Expression of mutant alanine tRNAs increases spontaneous mutagenesis in Escherichia coli
Q36578776Extreme dNTP pool changes and hypermutability in dcd ndk strains.
Q38711657Fluorescence-Based Reporters for Detection of Mutagenesis in E. coli
Q53022035Formulation of a nasogastric liquid feed and shelf-life extension using gamma radiation.
Q46717360Frameshift mutations induced by four isomeric nitroacridines and their des-nitro counterpart in the lacZ reversion assay in Escherichia coli
Q44556480Frameshift mutations induced by three classes of acridines in the lacZ reversion assay in Escherichia coli: potency of responses and relationship to slipped mispairing models
Q24816194Function of the ribosomal E-site: a mutagenesis study
Q35870401Functional characterization of human MutY homolog (hMYH) missense mutation (R231L) that is linked with hMYH-associated polyposis
Q33636195Functional expression of hMYH, a human homolog of the Escherichia coli MutY protein
Q39842494Generation of an endogenous DNA-methylating agent by nitrosation in Escherichia coli
Q35633393Genetic requirements and mutational specificity of the Escherichia coli SOS mutator activity
Q34719336Genomewide screen for modulators of evolvability under toxic antibiotic exposure.
Q42592813Genotoxicity and mutagenicity of Echinodorus macrophyllus (chapéu-de-couro) extracts.
Q39110732Helicobacter pylori genes involved in avoidance of mutations induced by 8-oxoguanine
Q39373211Hierarchy of lesion processing governs the repair, double-strand break formation and mutability of three-lesion clustered DNA damage
Q52334064High-accuracy lagging-strand DNA replication mediated by DNA polymerase dissociation.
Q38154090Highlights of the DNA cutters: a short history of the restriction enzymes
Q37319784Hypermutability and error catastrophe due to defects in ribonucleotide reductase
Q56288889Hypermutagenesis in mutA cells is mediated by mistranslational corruption of polymerase, and is accompanied by replication fork collapse
Q33601912Hypersensitivity of Escherichia coli Delta(uvrB-bio) mutants to 6-hydroxylaminopurine and other base analogs is due to a defect in molybdenum cofactor biosynthesis.
Q38503664Hypoxanthine incorporation is nonmutagenic in Escherichia coli
Q43720586Identification of antimutagenic properties of anthocyanins and other polyphenols from rose (Rosa centifolia) petals and tea.
Q33617930Identification of proteins of Escherichia coli and Saccharomyces cerevisiae that specifically bind to C/C mismatches in DNA.
Q33636589Identification of the miaB gene, involved in methylthiolation of isopentenylated A37 derivatives in the tRNA of Salmonella typhimurium and Escherichia coli
Q90711383Identifying Small Molecules That Promote Quasipalindrome-Associated Template-Switch Mutations in Escherichia coli
Q33993117Imbalanced base excision repair increases spontaneous mutation and alkylation sensitivity in Escherichia coli
Q44271868In vivo deamination of cytosine-containing cyclobutane pyrimidine dimers in E. coli: a feasible part of UV-mutagenesis
Q34363761In vivo mutagenesis
Q43958133Increased base change mutations at G:C pairs in Escherichia coli deficient in endonuclease III and VIII.
Q28268090Increased spontaneous mutation and alkylation sensitivity of Escherichia coli strains lacking the ogt O6-methylguanine DNA repair methyltransferase
Q43715473Induction of -2 frameshift mutations by 2-nitrofluorene, N-hydroxyacetylaminofluorene, and N-2-acetylaminofluorene in reversion assays in Escherichia coli strains differing in permeability and acetyltransferase activity
Q28750576Inferring clocks when lacking rocks: the variable rates of molecular evolution in bacteria
Q33993112Influence of S-adenosylmethionine pool size on spontaneous mutation, dam methylation, and cell growth of Escherichia coli
Q35065478Insights into mutagenesis using Escherichia coli chromosomal lacZ strains that enable detection of a wide spectrum of mutational events.
Q33467089Insights into the role of Val45 and Gln182 of Escherichia coli MutY in DNA substrate binding and specificity
Q44280600Interactions among the Escherichia coli mutT, mutM, and mutY damage prevention pathways
Q34697729Involvement of Y-family DNA polymerases in mutagenesis caused by oxidized nucleotides in Escherichia coli
Q79773161Isolation and analyses of MutY homologs (MYH)
Q42125751Isolation and characterization of Escherichia coli mutants with altered rates of deletion formation
Q39929800Isolation and characterization of an Escherichia coli strain with a high frequency of C-to-T mutations at 5-methylcytosines
Q45158692Isolation and characterization of bifunctional Escherichia coli TatA mutant proteins that allow efficient tat-dependent protein translocation in the absence of TatB.
Q34317246Lack of strand bias in UV-induced mutagenesis in Escherichia coli
Q50496578Lethality of visible light for Escherichia coli hemH1 mutants influence of defects in DNA repair.
Q35578593Liquid-based iterative recombineering method tolerant to counter-selection escapes
Q39502264Lowering S-adenosylmethionine levels in Escherichia coli modulates C-to-T transition mutations
Q45061765Mapping protein-protein interactions between MutL and MutH by cross-linking
Q35623813Maximization of transcription of the serC (pdxF)-aroA multifunctional operon by antagonistic effects of the cyclic AMP (cAMP) receptor protein-cAMP complex and Lrp global regulators of Escherichia coli K-12.
Q41110210Mechanisms of directed mutation
Q33847662Mechanisms of stationary phase mutation: a decade of adaptive mutation
Q39500646Metronidazole activation is mutagenic and causes DNA fragmentation in Helicobacter pylori and in Escherichia coli containing a cloned H. pylori RdxA(+) (Nitroreductase) gene
Q24605441Mismatch repair proteins collaborate with methyltransferases in the repair of O(6)-methylguanine
Q34135168Missense suppressor mutations in 16S rRNA reveal the importance of helices h8 and h14 in aminoacyl-tRNA selection
Q35115409Molecular devices for high fidelity of DNA replication and gene expression
Q24628966Multiple pathways for SOS-induced mutagenesis in Escherichia coli: an overexpression of dinB/dinP results in strongly enhancing mutagenesis in the absence of any exogenous treatment to damage DNA
Q35770316MutM, a protein that prevents G C→T A transversions, is formamidopyrimidine-DNA glycosylase
Q35848055MutY, an adenine glycosylase active on G-A mispairs, has homology to endonuclease III.
Q41192955Mutagen Synergy: Hypermutability Generated by Specific Pairs of Base Analogs
Q46007896Mutagenic potency of MMS-induced 1meA/3meC lesions in E. coli.
Q54350633Mutagenicity induced by UVC in Escherichia coli cells: reactive oxygen species involvement.
Q36145017Mutagenicity of 5-aza-2'-deoxycytidine is mediated by the mammalian DNA methyltransferase
Q37678547Mutants in the Exo I motif of Escherichia coli dnaQ: defective proofreading and inviability due to error catastrophe
Q40110327Mutation Frequencies at Defined Single Codon Sites in Vesicular Stomatitis Virus and Poliovirus Can Be Increased Only Slightly by Chemical Mutagenesis
Q72078193Mutation enhancing effect of o-vanillin in the lacZ gene of Escherichia coli: characterization of mutational spectrum
Q74645632Mutation spectra of chemical mutagens determined by Lac+ reversion assay with Escherichia coli WP3101P-WP3106P tester strains
Q41194789Mutational Consequences of Ciprofloxacin in Escherichia coli
Q102330204Mutational Spectra Induced by Flavonoid Extracts from Pepper Tree (Schinus terebinthifolius, Raddi) Stem Bark
Q34674682Mutational analysis of the 3'-->5' proofreading exonuclease of Escherichia coli DNA polymerase III
Q36501486Mutational consequences of dNTP pool imbalances in E. coli.
Q34075936Mutations at the accommodation gate of the ribosome impair RF2-dependent translation termination
Q35537125Mutations in the MutSalpha interaction interface of MLH1 can abolish DNA mismatch repair
Q70759379Mutations in the mutY gene of Escherichia coli enhance the frequency of targeted G:C-->T:a transversions induced by a single 8-oxoguanine residue in single-stranded DNA
Q43597497Mutations induced by 5-formyl-2'-deoxyuridine in Escherichia coli include base substitutions that can arise from mispairs of 5-formyluracil with guanine, cytosine and thymine
Q34976798Mutator and antimutator effects of the bacteriophage P1 hot gene product.
Q34077193Mutator phenotype resulting from DNA polymerase IV overproduction in Escherichia coli: preferential mutagenesis on the lagging strand
Q37637992Mutator tRNAs are encoded by the Escherichia coli mutator genes mutA and mutC: a novel pathway for mutagenesis
Q39958783Mycobacterium tuberculosis MutT1 (Rv2985) and ADPRase (Rv1700) proteins constitute a two-stage mechanism of 8-oxo-dGTP and 8-oxo-GTP detoxification and adenosine to cytidine mutation avoidance.
Q54570071Near ultraviolet radiation (UVA and UVB) causes a formamidopyrimidine glycosylase-dependent increase in G to T transversions.
Q35032008Near-ultraviolet mutagenesis in superoxide dismutase-deficient strains of Escherichia coli
Q40535793Negative regulation of DNA repair gene (ung) expression by the CpxR/CpxA two-component system in Escherichia coli K-12 and induction of mutations by increased expression of CpxR.
Q33923226New family of deamination repair enzymes in uracil-DNA glycosylase superfamily.
Q35922997Novel mutator mutants of E. coli nrdAB ribonucleotide reductase: insight into allosteric regulation and control of mutation rates
Q90574072Nucleoside Diphosphate Kinase Escalates A-to-C Mutations in MutT-Deficient Strains of Escherichia coli
Q35609925Overexpression of vsr in Escherichia coli is mutagenic
Q33931330Oversynthesis of a new Escherichia coli small RNA suppresses export toxicity of DsbA'-PhoA unfoldable periplasmic proteins
Q46078759Papillation in Bacillus anthracis colonies: a tool for finding new mutators
Q44327902Photomutagenicity of thiabendazole, a postharvest fungicide, in bacterial assays
Q35634443Physical and functional interactions between Escherichia coli MutY glycosylase and mismatch repair protein MutS
Q41910583Polynucleotide phosphorylase plays an important role in the generation of spontaneous mutations in Escherichia coli
Q38804830Potential Prophylactic Properties of Apple and Characterization of Potent Bioactive from cv. "Granny Smith" Displaying Strong Antimutagenicity in Models Including Human Lymphoblast TK6(+/-) Cell Line
Q77359720Potential of Escherichia coli GTP cyclohydrolase II for hydrolyzing 8-oxo-dGTP, a mutagenic substrate for DNA synthesis
Q27936494Primary sequence and biological functions of a Saccharomyces cerevisiae O6-methylguanine/O4-methylthymine DNA repair methyltransferase gene
Q41825528Processing of thymine glycol in a clustered DNA damage site: mutagenic or cytotoxic
Q43071817Production of 3-nitrosoindole derivatives by Escherichia coli during anaerobic growth
Q36752663Properties of plasmids responsible for production of extended-spectrum beta-lactamases
Q39846834Protection of DNA during oxidative stress by the nonspecific DNA-binding protein Dps.
Q73404201REV3 is required for spontaneous but not methylation damage-induced mutagenesis of Saccharomyces cerevisiae cells lacking O6-methylguanine DNA methyltransferase
Q24599371RNA virus error catastrophe: direct molecular test by using ribavirin
Q36339832Rate and molecular spectrum of spontaneous mutations in the bacterium Escherichia coli as determined by whole-genome sequencing.
Q33963800Rates of spontaneous mutation in bacteriophage T4 are independent of host fidelity determinants
Q28817569Redox Signaling through DNA
Q37340789Redox signaling between DNA repair proteins for efficient lesion detection
Q34510154Removal of 8-oxo-GTP by MutT hydrolase is not a major contributor to transcriptional fidelity
Q41943926Removal of deoxyinosine from the Escherichia coli chromosome as studied by oligonucleotide transformation
Q39749064Repair System for Noncanonical Purines in Escherichia coli
Q36560558Repair of 8-hydroxyguanine in DNA by mammalian N-methylpurine-DNA glycosylase
Q38583344Repair of DNA damage induced by bile salts in Salmonella enterica
Q33707101Repair of O4-alkylthymine by O6-alkylguanine-DNA alkyltransferases
Q33786744Requirement for Phe36 for DNA binding and mismatch repair by Escherichia coli MutS protein
Q27470003Response of foot-and-mouth disease virus to increased mutagenesis: influence of viral load and fitness in loss of infectivity
Q33639091Role of Escherichia coli DNA polymerase I in chromosomal DNA replication fidelity
Q35271644Role of Escherichia coli DNA polymerase IV in in vivo replication fidelity
Q29346764Role of Pseudomonas aeruginosa dinB-encoded DNA polymerase IV in mutagenesis
Q90205216Role of RNase H enzymes in maintaining genome stability in Escherichia coli expressing a steric-gate mutant of pol VICE391
Q36483171Role of accessory DNA polymerases in DNA replication in Escherichia coli: analysis of the dnaX36 mutator mutant
Q34626118Role of proofreading and mismatch repair in maintaining the stability of nucleotide repeats in DNA.
Q38313827Role of the N-terminal proline residue in the catalytic activities of the Escherichia coli Fpg protein
Q35822001SOS mutator activity: unequal mutagenesis on leading and lagging strands.
Q34615754Saturation of DNA mismatch repair and error catastrophe by a base analogue in Escherichia coli.
Q33256081Screening and characterization of variant Theta-class glutathione transferases catalyzing the activation of ethylene dibromide to a mutagen
Q82204338Screening and characterizing human NAT2 variants
Q41900983Search for proteins required for accurate gene expression under oxidative stress: roles of guanylate kinase and RNA polymerase
Q33586060Short-term signatures of evolutionary change in the Salmonella enterica serovar typhimurium 14028 genome
Q38347742Significance of the conserved amino acid sequence for human MTH1 protein with antimutator activity
Q30974317Simple sequence repeats and mucoid conversion: biased mucA mutagenesis in mismatch repair-deficient Pseudomonas aeruginosa.
Q41777210Single-strand DNA-specific exonucleases in Escherichia coli. Roles in repair and mutation avoidance.
Q39731504Site-specific protein modification to identify the MutL interface of MutH.
Q40733625Some features of base pair mismatch repair and its role in the formation of genetic recombinants.
Q34608581Some features of the mutability of bacteria during nonlethal selection
Q50134974Sources of spontaneous mutagenesis in bacteria.
Q36158946Specific A/G-to-C.G mismatch repair in Salmonella typhimurium LT2 requires the mutB gene product
Q35686019Specificity of the mutator caused by deletion of the yeast structural gene (APN1) for the major apurinic endonuclease
Q54704156Spectrum of mutations that occur under selective and non-selective conditions in E. coli
Q33963613Spontaneous mutation in Escherichia coli containing the dnaE911 DNA polymerase antimutator allele
Q61812524Stimulation of Replication Template-Switching by DNA-Protein Crosslinks
Q37355812Stress-induced beta-lactam antibiotic resistance mutation and sequences of stationary-phase mutations in the Escherichia coli chromosome.
Q39115482Structural and functional analysis of the MutS C-terminal tetramerization domain
Q34370938Structural/functional analysis of the human OXR1 protein: identification of exon 8 as the anti-oxidant encoding function.
Q36131550Structure of Escherichia coli K-12 miaA and characterization of the mutator phenotype caused by miaA insertion mutations
Q35451250Structure of Escherichia coli dGTP triphosphohydrolase: a hexameric enzyme with DNA effector molecules
Q44577322Suppression of SOS repair in E. coli: possible mechanism of antimutagenicity and protective effects of common vegetables
Q35562059Suppression of the E. coli SOS response by dNTP pool changes
Q46825076Tandem repeat mutation, global DNA methylation, and regulation of DNA methyltransferases in cultured mouse embryonic fibroblast cells chronically exposed to chemicals with different modes of action
Q90223851The Effect of 2-Thiocyanatopyridine Derivative 11026103 on Burkholderia Cenocepacia: Resistance Mechanisms and Systemic Impact
Q34657088The Escherichia coli MutL protein stimulates binding of Vsr and MutS to heteroduplex DNA.
Q90218788The Escherichia coli alkA Gene Is Activated to Alleviate Mutagenesis by an Oxidized Deoxynucleoside
Q34102902The Escherichia coli lacZ reversion mutagenicity assay
Q34687712The Escherichia coli methyl-directed mismatch repair system repairs base pairs containing oxidative lesions.
Q35630875The Vsr endonuclease of Escherichia coli: an efficient DNA repair enzyme and a potent mutagen
Q47999082The YggX protein of Salmonella enterica is involved in Fe(II) trafficking and minimizes the DNA damage caused by hydroxyl radicals: residue CYS-7 is essential for YggX function
Q34627664The mechanism of mutation induction by a hydrogen bond ambivalent, bicyclic N4-oxy-2'-deoxycytidine in Escherichia coli
Q39502867The miaA mutator phenotype of Escherichia coli K-12 requires recombination functions
Q42637285The mutT defect does not elevate chromosomal fragmentation in Escherichia coli because of the surprisingly low levels of MutM/MutY-recognized DNA modifications
Q34380582The observed human sperm mutation frequency cannot explain the achondroplasia paternal age effect
Q50158330The pleiotropic effects of his overexpression in Salmonella typhimurium do not involve AICAR-induced mutagenesis.
Q74808472The role of DNA damage in stationary phase ('adaptive') mutation
Q54333760The role of Fpg protein in UVC-induced DNA lesions.
Q41862287The roles of specific glycosylases in determining the mutagenic consequences of clustered DNA base damage
Q36731755The single-strand DNA binding activity of human PC4 prevents mutagenesis and killing by oxidative DNA damage
Q50143302The spectra of base substitutions induced by the impCAB, mucAB and umuDC error-prone DNA repair operons differ following exposure to methyl methanesulfonate
Q37425766The theta subunit of Escherichia coli DNA polymerase III: a role in stabilizing the epsilon proofreading subunit.
Q34218124Theoretical analysis of mutation hotspots and their DNA sequence context specificity
Q39808836Transcription increases multiple spontaneous point mutations in Salmonella enterica
Q44253207Tyr212: a key residue involved in strand discrimination by the DNA mismatch repair endonuclease MutH.
Q36283328Unequal fidelity of leading strand and lagging strand DNA replication on the Escherichia coli chromosome
Q33741202Uracil DNA glycosylase (UDG) activities in Bradyrhizobium diazoefficiens: characterization of a new class of UDG with broad substrate specificity
Q37469631Use of high throughput sequencing to observe genome dynamics at a single cell level
Q43790628Using base-specific Salmonella tester strains to characterize the types of mutation induced by benzidine and benzidine congeners after reductive metabolism
Q54318185Variety-based variation in the antimutagenic potential of various vegetables and lack of its correlation with their antioxidant capacity.
Q39503738Very-short-patch repair in Escherichia coli requires the dam adenine methylase
Q27013917Viral quasispecies evolution
Q34484982dnaX36 Mutator of Escherichia coli: effects of the {tau} subunit of the DNA polymerase III holoenzyme on chromosomal DNA replication fidelity.
Q33910770mutA and mutC: two mutator loci in Escherichia coli that stimulate transversions

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