Transient AID expression for in situ mutagenesis with improved cellular fitness.

scientific article published on 20 June 2018

Transient AID expression for in situ mutagenesis with improved cellular fitness. is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1038/S41598-018-27717-2
P932PMC publication ID6010430
P698PubMed publication ID29925928

P2093author name stringYu-Cheng Su
Steve R Roffler
Talal Salem Al-Qaisi
P2860cites workHistone chaperone Spt6 is required for class switch recombination but not somatic hypermutationQ24300395
Somatic hypermutation of the AID transgene in B and non-B cellsQ24535846
Activation-induced cytidine deaminase deaminates deoxycytidine on single-stranded DNA but requires the action of RNaseQ24683335
Expression of activation-induced cytidine deaminase in human B-cell non-Hodgkin lymphomasQ28201588
AID mutates E. coli suggesting a DNA deamination mechanism for antibody diversificationQ28208979
Hypermutation in human cancer genomes: footprints and mechanismsQ28255066
Hormone-sensitive lipase deficiency in mice causes diglyceride accumulation in adipose tissue, muscle, and testisQ28506864
Genome-wide mutation avalanches induced in diploid yeast cells by a base analog or an APOBEC deaminaseQ28536837
Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzymeQ29547201
Integrity of the AID serine-38 phosphorylation site is critical for class switch recombination and somatic hypermutation in miceQ30857687
Estimating Hypermutation Rates from Clonal Tree DataQ30882543
Why high-error-rate random mutagenesis libraries are enriched in functional and improved proteinsQ33216570
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Whole IgG surface display on mammalian cells: Application to isolation of neutralizing chicken monoclonal anti-IL-12 antibodiesQ33295087
Display and selection of scFv antibodies on HEK-293T cellsQ33474388
Genome-wide somatic hypermutationQ33905110
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Clustered and genome-wide transient mutagenesis in human cancers: Hypermutation without permanent mutators or loss of fitnessQ34090613
Coupling mammalian cell surface display with somatic hypermutation for the discovery and maturation of human antibodiesQ34096649
The B cell mutator AID promotes B lymphoid blast crisis and drug resistance in chronic myeloid leukemiaQ34099472
AID and somatic hypermutation.Q34118000
A source of the single-stranded DNA substrate for activation-induced deaminase during somatic hypermutationQ34133261
Affinity maturation of anti-TNF-alpha scFv with somatic hypermutation in non-B cellsQ34216459
Mimicking the germinal center reaction in hybridoma cells to isolate temperature-selective anti-PEG antibodies.Q34223906
High affinity humanized antibodies without making hybridomas; immunization paired with mammalian cell display and in vitro somatic hypermutationQ34482369
Coupling recombinase-mediated cassette exchange with somatic hypermutation for antibody affinity maturation in CHO cells.Q51741733
Somatic hypermutation in B cells: an optimal control treatment.Q52390653
Somatic hypermutation of immunoglobulin genes is linked to transcription initiationQ70934062
Affinity maturation and hypermutation in a simulation of the humoral immune responseQ71118314
Stages of germinal center transit are defined by B cell transcription factor coexpression and relative abundanceQ79326749
Efficient production of Cre-mediated site-directed recombinants through the utilization of the puromycin resistance gene, pac: a transient gene-integration marker for ES cellsQ34651786
Mammalian cell display and somatic hypermutation in vitro for human antibody discoveryQ34974395
Vectors bicistronically linking a gene of interest to the SV40 large T antigen in combination with the SV40 origin of replication enhance transient protein expression and luciferase reporter activityQ35184622
AIDing antibody diversity by error-prone mismatch repairQ36173942
Inter- and intraclonal diversity in the antibody response to influenza hemagglutininQ36355399
Clusters of Multiple Mutations: Incidence and Molecular MechanismsQ36455998
Germinal-center organization and cellular dynamicsQ36459345
Protein evolution by hypermutation and selection in the B cell line DT40Q36471434
Regulation of activation-induced cytidine deaminase DNA deamination activity in B-cells by Ser38 phosphorylationQ36519034
Antibody diversification caused by disrupted mismatch repair and promiscuous DNA polymerasesQ36540495
The biochemistry of somatic hypermutationQ37096080
Evolution of new nonantibody proteins via iterative somatic hypermutation.Q37627375
Activation induced deaminase C-terminal domain links DNA breaks to end protection and repair during class switch recombinationQ37659903
Protein engineering: a new frontier for biological therapeuticsQ38286271
APOBEC3 genes: retroviral restriction factors to cancer driversQ38395120
Protein engineering approaches to chemical biotechnologyQ38927933
A prevalent cancer susceptibility APOBEC3A hybrid allele bearing APOBEC3B 3'UTR enhances chromosomal DNA damageQ38949554
Directed evolution of green fluorescent protein by a new versatile PCR strategy for site-directed and semi-random mutagenesisQ39572601
Mutation drift and repertoire shift in the maturation of the immune responseQ39658890
Evolutionary and somatic selection of the antibody repertoire in the mouse.Q39682090
Mobilization of giant piggyBac transposons in the mouse genomeQ39826562
An ex vivo method for rapid generation of monoclonal antibodies (ADLib system).Q40150041
AID mutates a non-immunoglobulin transgene independent of chromosomal position.Q40303704
Induction of somatic hypermutation is associated with modifications in immunoglobulin variable region chromatinQ40625542
Generation and iterative affinity maturation of antibodies in vitro using hypermutating B-cell linesQ40697370
Increased transcription levels induce higher mutation rates in a hypermutating cell lineQ40814374
AID upmutants isolated using a high-throughput screen highlight the immunity/cancer balance limiting DNA deaminase activityQ40818553
High-throughput mutagenesis using a two-fragment PCR approachQ42339240
AID enzyme-induced hypermutation in an actively transcribed gene in fibroblastsQ42813803
Simultaneous surface display and secretion of proteins from mammalian cells facilitate efficient in vitro selection and maturation of antibodiesQ43097910
Processive AID-catalysed cytosine deamination on single-stranded DNA simulates somatic hypermutationQ44486460
Modern methods for laboratory diversification of biomoleculesQ47387337
Somatic hypermutation in the heavy chain locus correlates with transcriptionQ47713194
Clustered Mutation Signatures Reveal that Error-Prone DNA Repair Targets Mutations to Active GenesQ48016992
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P433issue1
P407language of work or nameEnglishQ1860
P304page(s)9413
P577publication date2018-06-20
P1433published inScientific ReportsQ2261792
P1476titleTransient AID expression for in situ mutagenesis with improved cellular fitness.
P478volume8

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cites work (P2860)
Q64250352Ex vivo evolution of human antibodies by CRISPR-X: from a naive B cell repertoire to affinity matured antibodies
Q94948553High efficiency CHO cell display-based antibody maturation
Q99712152Roadmap to Building a Cell: An Evolutionary Approach

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