Identification of a conserved Rac-binding site on NADPH oxidases supports a direct GTPase regulatory mechanism

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Identification of a conserved Rac-binding site on NADPH oxidases supports a direct GTPase regulatory mechanism is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1074/JBC.M801010200
P932PMC publication ID2442356
P698PubMed publication ID18347018

P2093author name stringDavide Gianni
Yu-Ya Kao
Gary M Bokoch
Ross M Taylor
Benjamin Bohl
P2860cites workCLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceQ24286950
A Ca(2+)-activated NADPH oxidase in testis, spleen, and lymph nodesQ24291514
Nox1-dependent reactive oxygen generation is regulated by Rac1Q24320021
Involvement of Rac1 in activation of multicomponent Nox1- and Nox3-based NADPH oxidasesQ24548021
Structure of the TPR domain of p67phox in complex with Rac.GTPQ27628646
The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiologyQ27860991
Cytochrome b558, a component of the phagocyte NADPH oxidase, is a flavoproteinQ67502906
Deficiency of cytochrome b558 in chronic granulomatous disease demonstrated by monoclonal antibody 7D5Q68086046
Monoclonal antibody 7D5 raised to cytochrome b558 of human neutrophils: immunocytochemical detection of the antigen in peripheral phagocytes of normal subjects, patients with chronic granulomatous disease, and their carrier mothersQ68949088
Activation of neutrophil NADPH oxidase in a cell-free system. Partial purification of components and characterization of the activation processQ68993049
Translocation of Rac correlates with NADPH oxidase activation. Evidence for equimolar translocation of oxidase componentsQ70500542
The cytosolic component p47(phox) is not a sine qua non participant in the activation of NADPH oxidase but is required for optimal superoxide productionQ71825234
X-CGDbase: a database of X-CGD-causing mutationsQ71877873
Shapes of MHC restrictionQ74451838
Missense mutations in the gp91-phox gene encoding cytochrome b558 in patients with cytochrome b positive and negative X-linked chronic granulomatous diseaseQ74588021
Principles of Fluorescence SpectroscopyQ106517819
Homologs of gp91phox: cloning and tissue expression of Nox3, Nox4, and Nox5Q28190953
Functional analysis of Nox4 reveals unique characteristics compared to other NADPH oxidasesQ28253777
Activation and assembly of the NADPH oxidase: a structural perspectiveQ28297425
Deficiency of the hematopoietic cell-specific Rho family GTPase Rac2 is characterized by abnormalities in neutrophil function and host defenseQ28609612
Tetratricopeptide repeat (TPR) motifs of p67(phox) participate in interaction with the small GTPase Rac and activation of the phagocyte NADPH oxidaseQ28609613
Rac2 is an essential regulator of neutrophil nicotinamide adenine dinucleotide phosphate oxidase activation in response to specific signaling pathwaysQ28609618
Regulation of phagocyte oxygen radical production by the GTP-binding protein Rac 2Q28609631
NOX enzymes and the biology of reactive oxygenQ29547517
Characterization of rac and cdc42 activation in chemoattractant-stimulated human neutrophils using a novel assay for active GTPasesQ29618793
NOXO1, Regulation of Lipid Binding, Localization, and Activation of Nox1 by the Phox Homology (PX) DomainQ30164408
NADPH oxidase activity is independent of p47phox in vitroQ30176826
Hematologically important mutations: X-linked chronic granulomatous disease (second update).Q31853269
Molecular basis for Rac2 regulation of phagocyte NADPH oxidaseQ32063730
The molecular basis for adhesion-mediated suppression of reactive oxygen species generation by human neutrophilsQ33630157
Assembly of the phagocyte NADPH oxidase: molecular interaction of oxidase proteinsQ34411802
Requirement for posttranslational processing of Rac GTP-binding proteins for activation of human neutrophil NADPH oxidaseQ34435975
Expression and activity of NOX5 in the circulating malignant B cells of hairy cell leukemia.Q34474580
The superoxide-generating oxidase of phagocytic cells. Physiological, molecular and pathological aspectsQ34507357
Current molecular models for NADPH oxidase regulation by Rac GTPaseQ34915029
NADPH oxidases: not just for leukocytes anymore!Q35296159
The NADPH oxidase of professional phagocytes--prototype of the NOX electron transport chain systemsQ35828194
Structure and regulation of the neutrophil respiratory burst oxidase: comparison with nonphagocyte oxidasesQ35829421
How to study proteins by circular dichroismQ36199360
A structural model for the nucleotide binding domains of the flavocytochrome b-245 beta-chainQ36277673
Role of the small GTPase Rac in p22phox-dependent NADPH oxidasesQ36855140
Molecular basis of chronic granulomatous diseaseQ37636984
Regulation of the neutrophil respiratory burst oxidase. Identification of an activation domain in p67(phox).Q38336129
Antagonistic cross-talk between Rac and Cdc42 GTPases regulates generation of reactive oxygen speciesQ40559852
Cytochrome b-245 is a flavocytochrome containing FAD and the NADPH-binding site of the microbicidal oxidase of phagocytesQ41619617
Cytochrome b558: the flavin-binding component of the phagocyte NADPH oxidase.Q41620128
p21-activated kinase (Pak) regulates NADPH oxidase activation in human neutrophilsQ42742659
Protein transduction: delivery of Tat-GTPase fusion proteins into mammalian cellsQ43576972
Creation of a genetic system for analysis of the phagocyte respiratory burst: high-level reconstitution of the NADPH oxidase in a nonhematopoietic systemQ43945169
Functional epitope on human neutrophil flavocytochrome b558.Q44470204
Human monocytes use Rac1, not Rac2, in the NADPH oxidase complexQ44545624
Direct involvement of the small GTPase Rac in activation of the superoxide-producing NADPH oxidase Nox1.Q45345920
Rac translocates independently of the neutrophil NADPH oxidase components p47phox and p67phox. Evidence for its interaction with flavocytochrome b558.Q46196991
Regulation of H2O2 generation in thyroid cells does not involve Rac1 activationQ46372081
Purification and characterization of Rac 2. A cytosolic GTP-binding protein that regulates human neutrophil NADPH oxidaseQ46500865
P433issue19
P407language of work or nameEnglishQ1860
P921main subjectcell biologyQ7141
superoxide-generating NAD(P)H oxidase activityQ14875829
NADPH oxidase 1Q21149712
P304page(s)12736-46
P577publication date2008-05-09
P1433published inJournal of Biological ChemistryQ867727
P1476titleIdentification of a conserved Rac-binding site on NADPH oxidases supports a direct GTPase regulatory mechanism
P478volume283

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cites work (P2860)
Q34181387A Conserved Region between the TPR and Activation Domains of p67 Participates in Activation of the Phagocyte NADPH Oxidase
Q33660799A novel and specific NADPH oxidase-1 (Nox1) small-molecule inhibitor blocks the formation of functional invadopodia in human colon cancer cells
Q34055834A prenylated p47phox-p67phox-Rac1 chimera is a Quintessential NADPH oxidase activator: membrane association and functional capacity
Q26991619Biochemistry, physiology, and pathophysiology of NADPH oxidases in the cardiovascular system
Q52671777Dock2 in the development of inflammation and cancer.
Q33649841Emerging evidence for the importance of phosphorylation in the regulation of NADPH oxidases
Q26861119Evolution of NADPH Oxidase Inhibitors: Selectivity and Mechanisms for Target Engagement
Q64097775Evolutionary and functional analysis of the plant-specific NADPH oxidase gene family in L
Q54316655Exendin-4 alleviates angiotensin II-induced senescence in vascular smooth muscle cells by inhibiting Rac1 activation via a cAMP/PKA-dependent pathway.
Q37412182Genetic silencing of Nox2 and Nox4 reveals differential roles of these NADPH oxidase homologues in the vasopressor and dipsogenic effects of brain angiotensin II.
Q42629466Inhibition of NADPH oxidase activation by peptides mapping within the dehydrogenase region of Nox2-A "peptide walking" study
Q48050484Innate immunity and the evolution of resistance to an emerging infectious disease in a wild bird
Q42698788NADPH oxidase expression and production of superoxide by human corneal stromal cells.
Q37589114NADPH oxidase in brain injury and neurodegenerative disorders
Q35977261NOX Modifiers-Just a Step Away from Application in the Therapy of Airway Inflammation?
Q50299247NOX3 complex:RAC1:GTP generates superoxide from oxygen
Q39596144Neuregulin induces HaCaT keratinocyte migration via Rac1-mediated NADPH-oxidase activation
Q35059397Nicotinamide adenine dinucleotide phosphate reduced oxidase 5 (Nox5) regulation by angiotensin II and endothelin-1 is mediated via calcium/calmodulin-dependent, rac-1-independent pathways in human endothelial cells
Q24316184Novel p47(phox)-related organizers regulate localized NADPH oxidase 1 (Nox1) activity
Q26861569Nox family NADPH oxidases in mechano-transduction: mechanisms and consequences
Q50299243Production of phagocyte oxygen radicals by NOX2 complex bound to RAC2:GTP
Q106518963RAC1 binds effectors at the plasma membrane
Q50299244RAC1:GTP binds NOX1 complex
Q50299246RAC1:GTP binds NOX3 complex
Q50299242RAC2:GTP binds NOX2 complex
Q49366921RCC2 over-expression in tumor cells alters apoptosis and drug sensitivity by regulating Rac1 activation.
Q42609911Rational design of small molecule inhibitors targeting the Rac GTPase-p67(phox) signaling axis in inflammation.
Q37139780Regulation of the phagocyte NADPH oxidase activity: phosphorylation of gp91phox/NOX2 by protein kinase C enhances its diaphorase activity and binding to Rac2, p67phox, and p47phox
Q37241059Role of Rac1 GTPase in JNK signaling and delayed neuronal cell death following global cerebral ischemia
Q41084118Role of Transient Receptor Potential Vanilloid 4 in Neutrophil Activation and Acute Lung Injury
Q38193605Role of the Rho GTPase Rac in the activation of the phagocyte NADPH oxidase: outsourcing a key task
Q38007895Strategies for identifying synthetic peptides to act as inhibitors of NADPH oxidases, or "all that you did and did not want to know about Nox inhibitory peptides".
Q28267075Structural insights into Nox4 and Nox2: motifs involved in function and cellular localization
Q37138949Suppressed hindlimb perfusion in Rac2-/- and Nox2-/- mice does not result from impaired collateral growth
Q30491877Targeting and regulation of reactive oxygen species generation by Nox family NADPH oxidases
Q36739856The involvement of the tyrosine kinase c-Src in the regulation of reactive oxygen species generation mediated by NADPH oxidase-1.
Q38007896Towards specific NADPH oxidase inhibition by small synthetic peptides
Q34361024c-Src-mediated phosphorylation of NoxA1 and Tks4 induces the reactive oxygen species (ROS)-dependent formation of functional invadopodia in human colon cancer cells
Q100153172ppDVL:RAC1:GTP binds NOX1 complex

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