Systematic mutagenesis of the Saccharomyces cerevisiae MLH1 gene reveals distinct roles for Mlh1p in meiotic crossing over and in vegetative and meiotic mismatch repair

scientific article

Systematic mutagenesis of the Saccharomyces cerevisiae MLH1 gene reveals distinct roles for Mlh1p in meiotic crossing over and in vegetative and meiotic mismatch repair is …
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scholarly articleQ13442814

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P356DOI10.1128/MCB.23.3.873-886.2003
P8608Fatcat IDrelease_56qc6vmttfbopjqsywwpobvbly
P932PMC publication ID140715
P698PubMed publication ID12529393

P50authorJuan Lucas ArguesoQ55457091
P2093author name stringEric Alani
Sumeet Sarin
Amanda Wraith Kijas
Marc Waase
Julie Heck
P2860cites workFunctional analysis of human MLH1 and MSH2 missense variants and hybrid human-yeast MLH1 proteins in Saccharomyces cerevisiaeQ24291685
Identification and characterization of Saccharomyces cerevisiae EXO1, a gene encoding an exonuclease that interacts with MSH2Q24314329
Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiaeQ24548535
Direct association of Bloom's syndrome gene product with the human mismatch repair protein MLH1Q24555123
Transformation of MutL by ATP binding and hydrolysis: a switch in DNA mismatch repairQ27617873
Crystal structure and ATPase activity of MutL: implications for DNA repair and mutagenesisQ27766073
Protein secondary structure prediction based on position-specific scoring matricesQ27860483
Mammalian Ras interacts directly with the serine/threonine kinase RafQ27860833
Functional studies on the candidate ATPase domains of Saccharomyces cerevisiae MutLalpha.Q27929951
Interaction between mismatch repair and genetic recombination in Saccharomyces cerevisiaeQ27930781
The Saccharomyces cerevisiae Msh2 and Msh6 proteins form a complex that specifically binds to duplex oligonucleotides containing mismatched DNA base pairsQ27930815
The budding yeast Msh4 protein functions in chromosome synapsis and the regulation of crossover distributionQ27931846
Characterization of insertion mutations in the Saccharomyces cerevisiae MSH1 and MSH2 genes: evidence for separate mitochondrial and nuclear functionsQ27932283
Discrete in vivo roles for the MutL homologs Mlh2p and Mlh3p in the removal of frameshift intermediates in budding yeastQ27934236
The Saccharomyces cerevisiae MLH3 gene functions in MSH3-dependent suppression of frameshift mutationsQ27935158
MSH-MLH complexes formed at a DNA mismatch are disrupted by the PCNA sliding clampQ27935260
exo1-Dependent mutator mutations: model system for studying functional interactions in mismatch repairQ27935389
High affinity cooperative DNA binding by the yeast Mlh1-Pms1 heterodimerQ27936605
Differential ATP binding and intrinsic ATP hydrolysis by amino-terminal domains of the yeast Mlh1 and Pms1 proteinsQ27937399
Eukaryotic DNA mismatch repairQ27939116
ATP-dependent assembly of a ternary complex consisting of a DNA mismatch and the yeast MSH2-MSH6 and MLH1-PMS1 protein complexesQ27939412
Functional specificity of MutL homologs in yeast: evidence for three Mlh1-based heterocomplexes with distinct roles during meiosis in recombination and mismatch correctionQ27939611
New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiaeQ28131599
Multifunctional yeast high-copy-number shuttle vectorsQ28131605
Exo1 roles for repair of DNA double-strand breaks and meiotic crossing over in Saccharomyces cerevisiaeQ28140402
MSH4 acts in conjunction with MLH1 during mammalian meiosisQ28142773
The Bloom's syndrome protein (BLM) interacts with MLH1 but is not required for DNA mismatch repairQ28186022
Differential timing and control of noncrossover and crossover recombination during meiosisQ28207440
DNA mismatch repair and mutation avoidance pathwaysQ28211143
Chromosomal influence on meiotic spindle assembly: abnormal meiosis I in female Mlh1 mutant miceQ28506066
The single-end invasion: an asymmetric intermediate at the double-strand break to double-holliday junction transition of meiotic recombinationQ29618523
The distribution of the numbers of mutants in bacterial populationsQ29620123
The Analysis of Tetrad DataQ31095632
Evidence for a physical interaction between the Escherichia coli methyl-directed mismatch repair proteins MutL and UvrD.Q32108861
Mammalian DNA mismatch repairQ33847720
Interactions of Exo1p with components of MutLalpha in Saccharomyces cerevisiaeQ33933718
Analysis of a gene conversion gradient at the HIS4 locus in Saccharomyces cerevisiaeQ33959781
Inactivation of DNA mismatch repair by increased expression of yeast MLH1.Q33967139
Biochemical Mutants in the Smut Fungus Ustilago Maydis.Q33975198
The many faces of mismatch repair in meiosisQ33984289
Isolation and characterization of point mutations in mismatch repair genes that destabilize microsatellites in yeastQ34012877
Mutations within the hMLH1 and hPMS2 subunits of the human MutLalpha mismatch repair factor affect its ATPase activity, but not its ability to interact with hMutSalphaQ34123282
CYS3, a hotspot of meiotic recombination in Saccharomyces cerevisiae. Effects of heterozygosity and mismatch repair functions on gene conversion and recombination intermediates.Q34606685
The conversion gradient at HIS4 of Saccharomyces cerevisiae. I. Heteroduplex rejection and restoration of Mendelian segregationQ34607928
EXO1 and MSH6 are high-copy suppressors of conditional mutations in the MSH2 mismatch repair gene of Saccharomyces cerevisiaeQ34609730
Decreased meiotic intergenic recombination and increased meiosis I nondisjunction in exo1 mutants of Saccharomyces cerevisiaeQ34611088
Analysis of conditional mutations in the Saccharomyces cerevisiae MLH1 gene in mismatch repair and in meiotic crossing over.Q34614513
MLH1 mutations differentially affect meiotic functions in Saccharomyces cerevisiaeQ34617068
Functional domains of the Saccharomyces cerevisiae Mlh1p and Pms1p DNA mismatch repair proteins and their relevance to human hereditary nonpolyposis colorectal cancer-associated mutationsQ36570003
The Saccharomyces cerevisiae ARG4 initiator of meiotic gene conversion and its associated double-strand DNA breaks can be inhibited by transcriptional interferenceQ37349528
Analysis of yeast MSH2-MSH6 suggests that the initiation of mismatch repair can be separated into discrete stepsQ38308734
Mlh1 is unique among mismatch repair proteins in its ability to promote crossing-over during meiosisQ38344945
Dominant negative mutator mutations in the mutL gene of Escherichia coliQ39716760
Meiotic Gene Conversion: A Signal of the Basic Recombination Event in YeastQ40613842
Instability of simple sequence DNA in Saccharomyces cerevisiaeQ40655124
Contribution of human mlh1 and pms2 ATPase activities to DNA mismatch repairQ40744193
Purification of eukaryotic MutL homologs from Saccharomyces cerevisiae using self-affinity technology.Q42427963
Repair of specific base pair mismatches formed during meiotic recombination in the yeast Saccharomyces cerevisiaeQ43182601
Mutation of a meiosis-specific MutS homolog decreases crossing over but not mismatch correctionQ48076989
Intermediates of Yeast Meiotic Recombination Contain Heteroduplex DNAQ58486283
Double-strand breaks at an initiation site for meiotic gene conversionQ59068287
An initiation site for meiotic gene conversion in the yeast Saccharomyces cerevisiaeQ59090272
Mismatch-specific post-meiotic segregation frequency in yeast suggests a heteroduplex recombination intermediateQ69893763
Origins of gene conversion and reciprocal exchange in AscobolusQ70974126
MSH5, a novel MutS homolog, facilitates meiotic reciprocal recombination between homologs in Saccharomyces cerevisiae but not mismatch repairQ71919357
The MutL ATPase is required for mismatch repairQ73586223
EXO1 and MSH4 differentially affect crossing-over and segregationQ73899517
Functional analysis of MLH1 mutations linked to hereditary nonpolyposis colon cancerQ77495184
The Escherichia coli MutL protein physically interacts with MutH and stimulates the MutH-associated endonuclease activityQ77765360
P4510describes a project that usesImageQuantQ112270642
P433issue3
P407language of work or nameEnglishQ1860
P921main subjectDNA mismatch repairQ2984243
Saccharomyces cerevisiaeQ719725
P304page(s)873-886
P577publication date2003-02-01
P1433published inMolecular and Cellular BiologyQ3319478
P1476titleSystematic mutagenesis of the Saccharomyces cerevisiae MLH1 gene reveals distinct roles for Mlh1p in meiotic crossing over and in vegetative and meiotic mismatch repair
P478volume23

Reverse relations

cites work (P2860)
Q36724317A mutation in the putative MLH3 endonuclease domain confers a defect in both mismatch repair and meiosis in Saccharomyces cerevisiae
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Q44780934Analysis of crossover breakpoints yields new insights into the nature of the gene conversion events associated with large NF1 deletions mediated by nonallelic homologous recombination
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Q34218228Combined fluorescent and electron microscopic imaging unveils the specific properties of two classes of meiotic crossovers.
Q34569381Competing crossover pathways act during meiosis in Saccharomyces cerevisiae
Q37533767Crossover patterning by the beam-film model: analysis and implications
Q27931634Csm4, in collaboration with Ndj1, mediates telomere-led chromosome dynamics and recombination during yeast meiosis
Q36371989Distinct DNA-binding surfaces in the ATPase and linker domains of MutLγ determine its substrate specificities and exert separable functions in meiotic recombination and mismatch repair
Q27659620Functional residues on the surface of the N-terminal domain of yeast Pms1
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Q33486516The pch2Delta mutation in baker's yeast alters meiotic crossover levels and confers a defect in crossover interference
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Q28253087Variation in crossover frequencies perturb crossover assurance without affecting meiotic chromosome segregation in Saccharomyces cerevisiae
Q42352313mlh3 mutations in baker's yeast alter meiotic recombination outcomes by increasing noncrossover events genome-wide.

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